CN104508114A - 用于在细胞中表达基因的启动子 - Google Patents

用于在细胞中表达基因的启动子 Download PDF

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Publication number
CN104508114A
CN104508114A CN201380032068.1A CN201380032068A CN104508114A CN 104508114 A CN104508114 A CN 104508114A CN 201380032068 A CN201380032068 A CN 201380032068A CN 104508114 A CN104508114 A CN 104508114A
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seq
promoter
sequence
dna
dna sequence
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Chinese (zh)
Inventor
埃里克·皮特·洛斯
威尔伯特·赫尔曼·马里·海涅
赫尔曼·扬·佩尔
罗波图斯·安东尼厄斯·戴维尔德
布伦达·沃恩克
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DSM IP Assets BV
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DSM IP Assets BV
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201380032068.1A 2012-06-19 2013-06-17 用于在细胞中表达基因的启动子 Pending CN104508114A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12172605 2012-06-19
EP12172605.3 2012-06-19
PCT/EP2013/062490 WO2013189878A1 (en) 2012-06-19 2013-06-17 Promoters for expressing a gene in a cell

Publications (1)

Publication Number Publication Date
CN104508114A true CN104508114A (zh) 2015-04-08

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CN201380032068.1A Pending CN104508114A (zh) 2012-06-19 2013-06-17 用于在细胞中表达基因的启动子

Country Status (6)

Country Link
US (1) US20150197760A1 (enExample)
EP (1) EP2861714A1 (enExample)
CN (1) CN104508114A (enExample)
BR (1) BR112014031526A2 (enExample)
IN (1) IN2014DN10111A (enExample)
WO (1) WO2013189878A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112795567A (zh) * 2020-12-24 2021-05-14 广东省微生物研究所(广东省微生物分析检测中心) 一种深海真菌FS140 O-甲基转移酶基因GliM启动子及其应用

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013191651A1 (en) * 2012-06-19 2013-12-27 Nanyang Technological University An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids
US20190330612A1 (en) 2016-06-30 2019-10-31 Danisco Us Inc Aspartic proteases
US10035997B2 (en) * 2016-07-21 2018-07-31 Fornia Biosolutions, Inc. G24 glucoamylase compositions and methods
US11634673B2 (en) * 2017-09-29 2023-04-25 Dupont Nutrition Biosciences Aps Production of brewer's wort having increase fermentable sugars for fermentation
WO2019173424A1 (en) 2018-03-09 2019-09-12 Danisco Us Inc Glucoamylases and methods of use thereof
CN110331144B (zh) * 2019-08-01 2023-01-20 天津科技大学 一种真菌启动子及其应用
CN113061538A (zh) * 2021-03-23 2021-07-02 江南大学 一种构巢曲霉自诱导型表达系统及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011054899A1 (en) * 2009-11-04 2011-05-12 Dsm Ip Assets B.V. Talaromyces transformants

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198345A (en) 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA1341226C (en) 1988-08-16 2001-05-01 Wim Van Hartingsveldt Gene replacement as a tool for the construction of aspergillus strains
WO1994004673A1 (en) 1992-08-19 1994-03-03 Alko Group Ltd. Fungal promoters active in the presence of glucose
EP0673429B1 (en) 1992-12-10 2002-06-12 Dsm N.V. Production of heterologous proteins in filamentous fungi
EP1321523A3 (en) 1993-07-23 2004-03-03 DSM IP Assets B.V. Selection marker gene free recombinant strains; a method for obtaining them and the use of these strains
CN1192108C (zh) 1994-06-03 2005-03-09 诺沃奇梅兹生物技术有限公司 纯化的毁丝霉属漆酶及编码该酶的核酸
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
KR100576576B1 (ko) 1997-02-20 2006-05-04 디에스엠 아이피 어셋츠 비.브이. 화학적 합성 배지를 이용하는 가치있는 화합물들의 산업적 규모의 발효적 생산
EP2298913A1 (en) 1997-04-11 2011-03-23 DSM IP Assets B.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
ATE367439T1 (de) 1997-12-22 2007-08-15 Dsm Ip Assets Bv Expressionsklonierung in filamentösen pilzen
WO2001021779A2 (en) 1999-09-17 2001-03-29 Dsm N.V. Penicillium chrysogenum transcriptional activator blar, a pathway specific regulator of beta-lactam biosynthesis, and uses therof
AU2005229437A1 (en) 2004-04-02 2005-10-13 Dsm Ip Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
CN102286483B (zh) 2004-04-16 2014-06-04 中化帝斯曼制药有限公司荷兰公司 用于在真菌细胞中表达基因的真菌启动子
US20080227148A1 (en) 2005-03-01 2008-09-18 Thibaut Jose Wenzel Aspergillus Promotors for Expressing a Gene in a Fungal Cell
JP2010517587A (ja) 2007-02-15 2010-05-27 ディーエスエム アイピー アセッツ ビー.ブイ. 関心のある化合物を生産するための組み換え宿主細胞
US20100120154A1 (en) 2007-03-21 2010-05-13 Dsm Ip Assets B.V. Method for homologous recombination
BRPI0911966B1 (pt) * 2008-05-11 2018-11-13 Univ Stellenbosch polinucleotídeo codificando uma celobioidrolase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011054899A1 (en) * 2009-11-04 2011-05-12 Dsm Ip Assets B.V. Talaromyces transformants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATRICK MURRAYA等: "Expression in Trichoderma reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus Talaromyces emersonii", 《PROTEIN EXPRESSION AND PURIFICATION》, vol. 38, no. 2, 31 December 2004 (2004-12-31), XP004649879, DOI: 10.1016/j.pep.2004.08.006 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112795567A (zh) * 2020-12-24 2021-05-14 广东省微生物研究所(广东省微生物分析检测中心) 一种深海真菌FS140 O-甲基转移酶基因GliM启动子及其应用
CN112795567B (zh) * 2020-12-24 2022-04-15 广东省微生物研究所(广东省微生物分析检测中心) 一种深海真菌FS140 O-甲基转移酶基因GliM启动子及其应用

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IN2014DN10111A (enExample) 2015-08-21
BR112014031526A2 (pt) 2017-08-01
EP2861714A1 (en) 2015-04-22
US20150197760A1 (en) 2015-07-16
WO2013189878A1 (en) 2013-12-27

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Application publication date: 20150408