CN104502619B - A kind of hydrophilic adheres to preparation method and the using method thereof of microscope slide - Google Patents
A kind of hydrophilic adheres to preparation method and the using method thereof of microscope slide Download PDFInfo
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- CN104502619B CN104502619B CN201410849438.7A CN201410849438A CN104502619B CN 104502619 B CN104502619 B CN 104502619B CN 201410849438 A CN201410849438 A CN 201410849438A CN 104502619 B CN104502619 B CN 104502619B
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Abstract
The invention discloses a kind of hydrophilic and adhere to preparation method and the using method thereof of microscope slide, described microscope slide is after amino silane solution that volumetric concentration is 0.8 8% or Poly-L-Lysine Solution pretreatment that volumetric concentration is 0.1 1%, washing, dry, it is soaked in again in the albumin solution that mass concentration is 0.5 5%, then takes out, washing, dry, obtain hydrophilic and adhere to microscope slide.Described hydrophilic slide surface has hydrophilic radical, good with the biomacromolecule compatibility, adhesion property is strong, and makes water-based reagent be prone to uniformly sprawl in slide surface, overcomes common adhesion and often occurs, when microscope slide uses on Active immunity group instrument, the uneven shortcoming that dyes.
Description
Technical field
The present invention relates to microscope slide field, be specifically related to a kind of hydrophilic and adhere to preparation method and the user thereof of microscope slide
Method.
Background technology
The most domestic common microscope slide that adheres to is that hydrophobicity adheres to microscope slide.This hydrophobicity adheres to microscope slide in Roche
When using on full-automatic SABC instrument, the uneven shortcoming that dyes often occurs.
The liquid capping film of Roche full-automatic SABC instrument is light oil, can adhere to microscope slide adhesion with hydrophobicity, lead
Cause dyeing uneven, make tissue to be measured that undyed white tiles to occur, cause the test crash of tissue.Therefore, hydrophobicity adheres to carry
Slide cannot be used on Roche full-automatic SABC instrument.And Roche full-automatic SABC instrument be commonly use at present complete from
Dynamic SABC instrument, accounts for 1/3rd of domestic full-automatic SABC instrument.Therefore, solve hydrophobicity adhesion microscope slide to make
The defect of used time, significant.
Summary of the invention
It is an object of the invention to overcome common adhesion, when microscope slide uses on Active immunity group instrument, dye often occurs
The shortcoming that color is uneven, it is provided that a kind of hydrophilic adheres to preparation method and the using method thereof of microscope slide.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in amino silane solution that volumetric concentration is 0.8-8% or volumetric concentration is 0.1-
In the Poly-L-Lysine Solution of 1%, soak 1-10min；
2) above-mentioned microscope slide is taken out, washing, dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 0.5-5%, soaks 1-10min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, washing, dry, obtain hydrophilic and adhere to microscope slide.
Further, the volumetric concentration of described amino silane solution is 2%.
The solvent of described amino silane solution is dehydrated alcohol, methanol or water.
Further, the volumetric concentration of described Poly-L-Lysine Solution is 0.1%.
Further, the mass concentration of described albumin solution is 1%.
Hydrophilic of the present invention adheres to the using method of microscope slide, comprises the following steps:
1), after tissue slice to be tested being become 3-5 micron, mount adheres on microscope slide at hydrophilic；
2) the above-mentioned microscope slide posting section is dried 2-24h in 65-70 DEG C；
3) microscope slide after above-mentioned drying is put into immunohistochemistry autostainer to test.
Described full-automatic SABC instrument is Roche full-automatic SABC instrument.
The present invention uses above technical scheme, described microscope slide through amino silane solution that volumetric concentration is 0.8-8% or
After volumetric concentration is the Poly-L-Lysine Solution pretreatment of 0.1-1%, then it is soaked in the albumin solution that mass concentration is 0.5-5%
In, then taking out, dry, the hydrophilic obtaining the present invention adheres to microscope slide.Microscope slide is soaked in albumin solution by the present invention
In, the multiple process for treating surface of this uniqueness makes slide surface have hydrophilic radical, good with the biomacromolecule compatibility,
Adhesion property is more preferably.Due to the existence of hydrophilic group, water-based reagent is made to be prone to uniformly sprawl in slide surface, particularly suitable sieve
Family name's full-automatic SABC instrument, overcomes common adhesion and often occurs when microscope slide uses on Roche full-automatic SABC instrument
Dye uneven shortcoming.
Detailed description of the invention
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in amino silane solution that volumetric concentration is 0.8-8% or volumetric concentration is 0.1-
In the Poly-L-Lysine Solution of 1%, soak 1-10min；
2) above-mentioned microscope slide is taken out, washing, dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 0.5-5%, soaks 1-10min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, washing, dry, obtain hydrophilic and adhere to microscope slide.
The solvent of described amino silane solution is dehydrated alcohol, methanol or water.
Described hydrophilic adheres to the using method of microscope slide, comprises the following steps:
1), after tissue slice to be tested being become 3-5 micron, mount adheres on microscope slide at hydrophilic；
2) the above-mentioned microscope slide posting section is dried 2-24h in 65-70 DEG C；
3) microscope slide after above-mentioned drying is put into full-automatic SABC instrument to test.
Embodiment 1
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in (2mL amino silicone in the amino silane ethanol solution that volumetric concentration is 2%
Alkane is dissolved in 98mL dehydrated alcohol), soak 10min；
2) above-mentioned microscope slide is taken out, wash (being soaked in water), dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 1%, soaks 10min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, wash (being soaked in water), dry, obtain hydrophilic
Adhere to microscope slide.
Embodiment 2
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in (0.8mL ammonia in the amino silane ethanol solution that volumetric concentration is 0.8%
Base silane is dissolved in 99.2mL dehydrated alcohol), soak 1min；
2) above-mentioned microscope slide is taken out, wash (being soaked in water), dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 0.5%, soaks 1min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, wash (being soaked in water), dry, obtain hydrophilic
Adhere to microscope slide.
Embodiment 3
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in (8mL amino silicone in the amino silane ethanol solution that volumetric concentration is 8%
Alkane is dissolved in 92mL dehydrated alcohol), soak 5min；
2) above-mentioned microscope slide is taken out, washing, dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 5%, soaks 5min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, washing, dry, obtain hydrophilic and adhere to microscope slide.
Embodiment 4
A kind of hydrophilic adheres to the preparation method of microscope slide, first prepares the amino silane methanol solution that volumetric concentration is 2%
In (2mL amino silane is dissolved in 98mL methanol), other step uses method same as in Example 1.
Embodiment 5
A kind of hydrophilic adheres to the preparation method of microscope slide, first prepares the amino silane methanol that volumetric concentration is 0.8% molten
In liquid (0.8mL amino silane is dissolved in 99.2mL methanol), other step uses method same as in Example 2.
Embodiment 6
A kind of hydrophilic adheres to the preparation method of microscope slide, first prepares the amino silane methanol solution that volumetric concentration is 8%
In (8mL amino silane is dissolved in 92mL methanol), other step uses method same as in Example 3.
Embodiment 7
A kind of hydrophilic adheres to the preparation method of microscope slide, first prepares in the amino silane aqueous solution that volumetric concentration is 2%
(2mL amino silane is dissolved in 98mL water), other step uses method same as in Example 1.
Embodiment 8
A kind of hydrophilic adheres to the preparation method of microscope slide, first prepares the amino silane aqueous solution that volumetric concentration is 0.8%
In (0.8mL amino silane is dissolved in 99.2mL water), other step uses method same as in Example 2.
Embodiment 9
A kind of hydrophilic adheres to the preparation method of microscope slide, first prepares in the amino silane aqueous solution that volumetric concentration is 8%
(8mL amino silane is dissolved in 92mL water), other step uses method same as in Example 3.
Embodiment 10
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in the Poly-L-Lysine Solution that volumetric concentration is 0.5% (0.5mL poly relies ammonia
Acid is dissolved in 99.5mL distilled water), soak 10min；
2) above-mentioned microscope slide is taken out, washing, dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 1%, soaks 10min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, washing, dry, obtain hydrophilic and adhere to microscope slide.
Embodiment 11
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in the Poly-L-Lysine Solution that volumetric concentration is 0.1%, soaks 1min；
2) above-mentioned microscope slide is taken out, washing, dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 0.5%, soaks 1min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, washing, dry, obtain hydrophilic and adhere to microscope slide.
Embodiment 12
A kind of hydrophilic adheres to the preparation method of microscope slide, comprises the following steps:
1) microscope slide of cleaning is soaked in the Poly-L-Lysine Solution that volumetric concentration is 1%, soaks 5min；
2) above-mentioned microscope slide is taken out, washing, dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 5%, soaks 5min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, washing, dry, obtain hydrophilic and adhere to microscope slide.
Embodiment 13
Described hydrophilic adheres to the using method of microscope slide, comprises the following steps:
1), after tissue slice to be tested being become 3-5 micron, mount adheres on microscope slide at hydrophilic；
2) the above-mentioned microscope slide posting section is dried 24h in 65 DEG C；
3) microscope slide after above-mentioned drying is put into Roche full-automatic SABC instrument to test.
Embodiment 14
Described hydrophilic adheres to the using method of microscope slide, comprises the following steps:
1), after tissue slice to be tested being become 3-5 micron, mount adheres on microscope slide at hydrophilic；
2) the above-mentioned microscope slide posting section is dried 24h in 70 DEG C；
3) microscope slide after above-mentioned drying is put into full-automatic SABC instrument to test.
Claims (4)
1. the preparation method of a hydrophilic adhesion microscope slide, it is characterised in that: it comprises the following steps:
1) microscope slide is soaked in the amino silane solution that volumetric concentration is 2% or the poly-D-lysine that volumetric concentration is 0.1% is molten
In liquid, soak 1-10min；
2) above-mentioned microscope slide is taken out, washing, dry, obtain adhering to microscope slide；
3) above-mentioned adhesion microscope slide is soaked in the albumin solution that mass concentration is 1%, soaks 1-10min；
4) microscope slide after above-mentioned immersion albumin solution is taken out, washing, dry, obtain hydrophilic and adhere to microscope slide.
A kind of hydrophilic the most according to claim 1 adheres to the preparation method of microscope slide, it is characterised in that: described amino silicone
The solvent of alkane solution is dehydrated alcohol, methanol or water.
A kind of hydrophilic the most as claimed in claim 1 or 2 adhere to microscope slide using method, it is characterised in that: it include with
Lower step:
1) by after tissue slice to be tested, mount adheres on microscope slide at hydrophilic；
2) the above-mentioned microscope slide posting section is dried 2-24h in 65-70 DEG C；
3) microscope slide after above-mentioned drying is put into full-automatic SABC instrument to test.
A kind of hydrophilic the most according to claim 3 adhere to microscope slide using method, it is characterised in that: described automatically
SABC instrument is Roche full-automatic SABC instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410849438.7A CN104502619B (en) | 2014-12-31 | 2014-12-31 | A kind of hydrophilic adheres to preparation method and the using method thereof of microscope slide |
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| CN201410849438.7A CN104502619B (en) | 2014-12-31 | 2014-12-31 | A kind of hydrophilic adheres to preparation method and the using method thereof of microscope slide |
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| CN104502619A CN104502619A (en) | 2015-04-08 |
| CN104502619B true CN104502619B (en) | 2016-09-21 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106478973A (en) * | 2016-08-31 | 2017-03-08 | 宁波华莱斯医疗器械有限公司 | A kind of hydrophily slide and preparation method thereof |
| CN107063812A (en) * | 2017-03-30 | 2017-08-18 | 南通大学 | A kind of low flake rate of liquid-based culture hydrophily adheres to the preparation method of slide |
| CN108641634B (en) * | 2018-06-22 | 2020-06-02 | 杭州医国仁生物科技有限公司 | Adhesive composition for slide glass coating and single-side coating process |
| CN110132668B (en) * | 2019-04-28 | 2022-02-15 | 西安培华学院 | Conventional glass slide super-hydrophobic treatment method |
| CN110426257A (en) * | 2019-08-13 | 2019-11-08 | 南通美韦德生命科学有限公司 | A kind of adherency glass slide and preparation method thereof to fall off with high adhesive capacity preventing tissue |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5453381A (en) * | 1993-12-10 | 1995-09-26 | Lipton; Stewart | Method for adhering fecal samples to slides |
| MX2007012407A (en) * | 2005-04-15 | 2008-01-22 | Lee H Angros | Analytic substrate coating apparatus and method. |
| CN103253873B (en) * | 2012-02-17 | 2015-03-18 | 麦克奥迪（厦门）医疗诊断系统有限公司 | Glass slide adhesive |
| CN104142262B (en) * | 2014-07-22 | 2017-03-29 | 中国矿业大学 | A kind of method in slide surface fixed dna base |
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