CN103253873B - Glass slide adhesive - Google Patents
Glass slide adhesive Download PDFInfo
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- CN103253873B CN103253873B CN201210036368.4A CN201210036368A CN103253873B CN 103253873 B CN103253873 B CN 103253873B CN 201210036368 A CN201210036368 A CN 201210036368A CN 103253873 B CN103253873 B CN 103253873B
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- glass slide
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- dyeing
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Abstract
The invention discloses a glass slide adhesive. The glass slide adhesive includes 0.01-0.2% by volume of 3-aminopropyltriethoxysilane (APTES), 0.01-3% by volume of a hydrophilic reagent, 0.005-0.05% by volume of a stabilizer, 0.5-5% by volume of alcohol, and the balance ultrapure water. The glass slide adhesive makes cells and tissue slices be firmly adhered on a glass slide, prevents the shedding of the cells and the tissue slices from the glass slide because of peracidity, high temperature, high pressure and the like in a dyeing process, overcomes the disadvantages of bad adhesiveness and weak wettability of general adhesives when the general adhesives are used for the glass slide processing in practical work, is suitable for the tissue slices, routine cell smears, fluid-based cytologic preparation, and manual drops, and has the advantages of good glass slide adherence effect, long preservation time, low cost, and high popularization values.
Description
Technical field
The present invention relates to one makes cell, tissue slice be affixed on securely on slide glass, prevents in dyeing course because the factors such as peracid, high pressure causes falling the slide glass anticreep reagent treatment of sheet.
Background technology
Pathology technique makes rapid progress, especially development in recent years immunohistochemical methods, liquid based cytology rapidly.In dyeing course, because cell, tissue slice will experience the dyeing of various different methods, when especially running into the dyeing of the complexity such as specific stain, Feulgen dyeing, immunohistochemical methods, often affect by factors such as high temperature, high pressure, radiation, acid and alkali corrosions, histocyte very easily comes off from slide glass.Through bad adhesive agent process, also may there is the problems such as slide background coloration, pollution in slide.The not only routine work of influence technique personnel, is also unfavorable for the diagnosis of pathologist.
Summary of the invention
The object of the invention is to, there is provided one that cell, tissue slice are adhered on slide glass securely, to prevent from dyeing course, because the factors such as peracid, high temperature, high pressure cause falling the Novel glass slide adhesive agent of sheet, overcoming in real work and often running into the shortcoming that general adhesive agent is poor for adhesivity during slide glass process, affinity is not strong.
In order to reach above-mentioned purpose, technical scheme of the present invention is:
A kind of slide glass adhesive agent, is characterized in that by volume per-cent comprises:
3-aminopropyl triethoxysilane (APTES) 0.01%-0.2%,
Hydrophilic reagent 0.01%-3%,
Stablizer 0.005%-0.05%,
Alcohol 0.5%-5%,
Ultrapure water supplies 100%.
Above-mentioned hydrophilic reagent is the one in cocoanut fatty acid diethanolamide, polyvinylpyrrolidone (PVP) and triton x-100.
Aforementioned stable agent is at least the one in Macrogol 200 and Macrogol 4000.
Above-mentioned alcohol is at least the one in ethanol, n-propyl alcohol, glycerol or Virahol.
After adopting such scheme, the present invention has the following advantages:
One, this silylating reagent is by playing chemically modified effect to glass surface, changes the chemico-physical properties of glass substrate surface, forms stable associative key, make cell, section and slide glass combination more firm;
Two, unique introducing hydrophilization technology, can improve slide glass affinity, make liquid and surface of glass slide contact angle θ be tending towards 0 °, increase liquid velocity of diffusion, so that what cell energy was even, single is distributed in the visual field, is not only applicable to tissue slice, be also applicable to liquid based cytology film-making;
Three, adding applicable stablizer, is the important guarantee that slide keeps lasting affinity;
Four, silanization treatment technology has the feature of environmental protection (without toxic heavy metal ion), less energy-consumption (normal temperature use), low use cost.
Embodiment
Embodiment 1:
According to the form below weighs or measures each component, is mixed with 100ml solution,
3-aminopropyl triethoxysilane (APTES) 0.010ml
Cocoanut fatty acid diethanolamide 0.200ml
Macrogol 200 0.010ml
Macrogol 4000 (density 1.212g/ml) 0.020g
Virahol 1.000ml
Ultrapure water supplies 100ml
Stirring and dissolving after each component mixing, slide glass soaks 5-10 minute, dries.
Process 400 slide glasss, carry out cytology, histology film-making, adopt normal dyeing, Feulgen dyeing etc.Result shows various film-making, dyeing process cell falls sheet rate and is 0%; Liquid based cytology film-making, affinity is poor; After process, surface of glass slide degree of cleaning are poor.
Embodiment 2:
According to the form below weighs or measures each component, is mixed with 100ml solution,
3-aminopropyl triethoxysilane (APTES) 0.100ml
Polyvinylpyrrolidone (PVP) (density 1.23-1.29g/ml) 0.200g
Macrogol 200 0.005ml
Ethanol 0.500ml
Glycerol 1.650ml
Ultrapure water supplies 100ml
Stirring and dissolving after each component mixing, slide glass soaks 5-10 minute, dries.
Process 400 slide glasss, carry out cytology, histology film-making, adopt normal dyeing, Feulgen dyeing etc.Result shows various film-making, dyeing process cell falls sheet rate and is 0%; Liquid based cytology film-making, affinity is poor; After process, surface of glass slide degree of cleaning are good.
Embodiment 3:
According to the form below weighs or measures each component, is mixed with 100ml solution,
3-aminopropyl triethoxysilane (APTES) 0.020ml
Polyvinylpyrrolidone (PVP) (density 1.23-1.29g/ml) 0.300g
Macrogol 4000 (density 1.212g/ml) 0.010g
Virahol 2.000ml
Ultrapure water supplies 100ml
Stirring and dissolving after each component mixing, slide glass soaks 5-10 minute, dries.
Process 400 slide glasss, carry out cytology, histology film-making, adopt normal dyeing, Feulgen dyeing etc.Result shows various film-making, dyeing process cell falls sheet rate and is 0%; Liquid based cytology film-making, affinity is strong; After process, surface of glass slide degree of cleaning are good.
Embodiment 4:
According to the form below weighs or measures each component, is mixed with 100ml solution,
3-aminopropyl triethoxysilane (APTES) 0.200ml
Triton x-100 0.030ml
Macrogol 4000 (density 1.2g/ml) 0.050g
Virahol 5.000ml
Ultrapure water supplies 100ml
Stirring and dissolving after each component mixing, slide glass soaks 5-10 minute, dries.
Process 400 slide glasss, carry out cytology, histology film-making, adopt normal dyeing, Feulgen dyeing etc.Result shows various film-making, dyeing process cell falls sheet rate and is 0%; Liquid based cytology film-making, affinity is strong; After process, surface of glass slide degree of cleaning are good.
Embodiment 5:
According to the form below weighs or measures each component, is mixed with 100ml solution,
3-aminopropyl triethoxysilane (APTES) 0.200ml
Polyvinylpyrrolidone (PVP) (density 1.2g/ml) 3.000g
Macrogol 200 0.020ml
Glycerol 1.000ml
Ultrapure water supplies 100ml
Stirring and dissolving after each component mixing, slide glass soaks 5-10 minute, dries.
Process 400 slide glasss, carry out cytology, histology film-making, adopt normal dyeing, Feulgen dyeing etc.Result shows various film-making, dyeing process cell falls sheet rate and is 0%; Liquid based cytology film-making, affinity is good; After process, surface of glass slide degree of cleaning are poor.
Embodiment 6:
According to the form below weighs or measures each component, is mixed with 100ml solution,
3-aminopropyl triethoxysilane (APTES) 0.010ml
Cocoanut fatty acid diethanolamide 0.200ml
Macrogol 4000 (density 1.2g/ml) 0.020g
N-propyl alcohol 2.000ml
Ultrapure water supplies 100ml
Stirring and dissolving after each component mixing, slide glass soaks 5-10 minute, dries.
Process 400 slide glasss, carry out cytology, histology film-making, adopt normal dyeing, Feulgen dyeing etc.Result shows various film-making, dyeing process cell falls sheet rate and is 0%; Liquid based cytology film-making, affinity is poor; After process, surface of glass slide degree of cleaning are poor.
Utilize the slide glass of process of the present invention, in histology, cytology film-making process, can not organize, situation that cell falls sheet, show that the slide glass adhesion property after this invention process is splendid.In cytology film-making, embodiment 3,4 has fabulous parent and moistens effect, and after process, surface of glass slide is clean.
Claims (2)
1. a slide glass adhesive agent, is characterized in that by volume per-cent comprises:
3-aminopropyl triethoxysilane 0.01%-0.2%,
Hydrophilic reagent 0.01%-3%,
Stablizer 0.005%-0.05%,
Alcohol 0.5%-5%,
Ultrapure water supplies 100%;
Described hydrophilic reagent is the one in coco-nut oil fatty acid, diglycollic amide, polyvinylpyrrolidone and triton x-100; Described stablizer is at least the one in Macrogol 200 and Macrogol 4000.
2. according to a kind of slide glass adhesive agent described in claim 1, it is characterized in that: alcohol is at least the one in ethanol, n-propyl alcohol, glycerol or Virahol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210036368.4A CN103253873B (en) | 2012-02-17 | 2012-02-17 | Glass slide adhesive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210036368.4A CN103253873B (en) | 2012-02-17 | 2012-02-17 | Glass slide adhesive |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103253873A CN103253873A (en) | 2013-08-21 |
| CN103253873B true CN103253873B (en) | 2015-03-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210036368.4A Active CN103253873B (en) | 2012-02-17 | 2012-02-17 | Glass slide adhesive |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104176945B (en) * | 2014-09-02 | 2016-08-17 | 安徽信灵检验医学科技有限公司 | A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof |
| CN104502619B (en) * | 2014-12-31 | 2016-09-21 | 沈洪武 | A kind of hydrophilic adheres to preparation method and the using method thereof of microscope slide |
| CN107935403A (en) * | 2017-12-10 | 2018-04-20 | 盐城市耀华玻璃仪器厂 | A kind of anticreep microslide and preparation method thereof |
| CN108033690A (en) * | 2017-12-10 | 2018-05-15 | 盐城市耀华玻璃仪器厂 | One kind adhesion glass slide and its production technology |
| CN109212738A (en) * | 2018-08-17 | 2019-01-15 | 盐城市耀华玻璃仪器厂 | A kind of anti-skidding glass slide |
| CN110426259A (en) * | 2019-08-14 | 2019-11-08 | 武汉赛维尔生物科技有限公司 | Polyethylene glycol is used for the application of animal tissue sections grease dyeing |
| CN112945671A (en) * | 2021-02-07 | 2021-06-11 | 南昌大学附属口腔医院(江西省口腔医院) | Adhesive glass slide and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6548263B1 (en) * | 1997-05-29 | 2003-04-15 | Cellomics, Inc. | Miniaturized cell array methods and apparatus for cell-based screening |
| CN101048661A (en) * | 2004-08-17 | 2007-10-03 | 生物概念股份有限公司 | Protein microarrays |
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2012
- 2012-02-17 CN CN201210036368.4A patent/CN103253873B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6548263B1 (en) * | 1997-05-29 | 2003-04-15 | Cellomics, Inc. | Miniaturized cell array methods and apparatus for cell-based screening |
| CN101048661A (en) * | 2004-08-17 | 2007-10-03 | 生物概念股份有限公司 | Protein microarrays |
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| CN103253873A (en) | 2013-08-21 |
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