CN104176945B - A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof - Google Patents
A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof Download PDFInfo
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- CN104176945B CN104176945B CN201410443831.6A CN201410443831A CN104176945B CN 104176945 B CN104176945 B CN 104176945B CN 201410443831 A CN201410443831 A CN 201410443831A CN 104176945 B CN104176945 B CN 104176945B
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Abstract
The invention discloses a kind of cation membranization dressing agent and the preparation method of anticreep slide thereof, the composition by weight of described cation membranization dressing agent, including poly-dopamine 1.5～2.5 parts, L lysine 0.5～1.5 parts, polyvinylpyrrolidone 1.5～2.5 parts, cohesion amine 1.5～2.5 parts, Sanguis Bovis seu Bubali albumin 0.5～1.5 parts and ethanol 89.5～94.5 parts, described preparation method includes slide first by coupling agent treatment, process with described cation membranization dressing agent again, it is thus achieved that anticreep slide；Compared with prior art, the anticreep slide cell capture effect of the present invention is good, good anti-drop effect, provides optimum specimen amount for later phase clinical pathological analysis.
Description
Technical field
The present invention relates to the Cell sheet glass of cell supporting body during the inspection of a kind of clinical medicine, in particular a kind of sun from
Sub-membranization dressing agent and the preparation method of anticreep slide thereof.
Background technology
Anticreep slide is again ion modification slide and cell capture slide etc., is to microscope slide or coverslip by chemically or physically method
Surface modify, to prevent cell or tissue in operating process from falling the generation of sheet phenomenon.Anticreep slide is applied widely:
If pathological tissue section, exfoliative cyte film-making are, and cell is cultivated, particularly liquid basal cell thin layer during cervical scraping smear
Film-making etc..The use kind of anticreep slide is the most various: such as cation anticreep slide, anion anticreep slide, hydroformylation anticreep slide
And the silica glass surface treated product etc. such as silication anticreep slide.The processing method of anticreep surface of glass slide also has multiple: such as physics side
The ultrasound wave of method, the abluent of chemical method or chemical modifier, or physical method and chemical method combination.Wherein, use
Chemical modifier (binding agent) also have difference, such as: poly-l-lysine (Poly-L-lysine), silane finish (3-
Aminopropyl-(ethoxymethyl) silane).But under the two-dimensional structure keyed jointing of above-mentioned this plane is inefficient, all can not be fully solved mark
This flake problem.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of cation membranization dressing agent and anticreep slide thereof
Preparation method, to solve the anticreep inefficient technical problem of slide anticreep under existing planar structural bond effect.
The present invention is achieved by the following technical solutions:
The invention provides a kind of cation membranization dressing agent, by weight, described cation membranization dressing agent includes following components:
Weigh the component raw material of above-mentioned parts by weight, mixing also ultrasonic dissolution assisting, until completely dissolved, the membrane filtration of 0.3 μm,
Obtain described cation membranization dressing agent.
As a kind of preferred version, described cation membranization dressing agent by weight, including following components:
Present invention also offers a kind of anticreep slide utilizing above-mentioned cation membranization dressing agent to prepare, the preparation of described anticreep slide
Method, comprises the following steps:
(1) clean alkalization slide is immersed process 2 minutes in coupling agent, in 37 DEG C of drying after vertical taking-up, it is thus achieved that surface
The slide of activation；
(2) slide of the surface active of step (1) is vertically put in above-mentioned cation membranization dressing agent and soak 10 minutes, hang down
In 37 DEG C of drying after straight taking-up, it is thus achieved that described anticreep slide；Described anticreep slide aluminum film phonograph seal, preserves at 4～8 DEG C.
3-aminopropyl-(ethoxymethyl) silane selected by the coupling agent of described step (1).
The principle of the present invention is: the present invention first by cationic polymer by surface-crosslinked technology, by multiple cationic polymer
Group's overlap membranization is in surface of glass slide so that the surface of slide exists the space cationic functional group of substantial amounts of three-dimensional, increases
Add positive charge and the corresponding function group receptor of anticreep surface of glass slide, in non-ionic buffer, made Clinical Laboratory electronegative
The target cell of lotus can adsorb mutually with the cation membranization layer of this anticreep surface of glass slide, makes one layer of equally distributed cell thin,
Prevent cell detachment.
The present invention has the advantage that compared to existing technology and the invention provides a kind of cation membranization dressing agent and anticreep slide thereof
Preparation method, this anticreep slide is the three dimensions cationic functional group utilizing its surface to exist in a large number, with electronegative
Target cell adsorbs mutually, and its keyed jointing efficiency anticreep slide common compared to existing market has significant difference, its cell capture
Effect is good, provides optimum specimen amount for later phase clinical pathological analysis.
Detailed description of the invention
Elaborating embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention,
Give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
1, the preparation of cation membranization dressing agent:
By weight, the component raw material of following number is weighed:
By the above-mentioned raw material weighed mixing and ultrasonic, after solute is completely dissolved, the membrane filtration of 0.3 μm, it is thus achieved that described sun from
Sub-membranization dressing agent.
2, the preparation of anticreep slide:
(1) clean alkalization slide is immersed process 2 minutes in coupling agent 3-aminopropyl-(ethoxymethyl) silane, after vertical taking-up
In 37 DEG C of drying, it is thus achieved that the slide of surface active；
(2) slide of the surface active of step (1) is vertically put in above-mentioned cation membranization dressing agent and soak 10 minutes, hang down
In 37 DEG C of drying after straight taking-up, it is thus achieved that described anticreep slide, by this anticreep slide aluminum film phonograph seal, save backup at 4～8 DEG C.
Embodiment 2
The preparation method of the anticreep slide that the cation membranization dressing agent of the present embodiment processes, wherein, described cation membranization is modified
Agent by weight, including following components:
Other steps are with embodiment 1.
Embodiment 3
The preparation method of the anticreep slide that the cation membranization dressing agent of the present embodiment processes, wherein, described cation membranization is modified
Agent by weight, including following components:
Other steps are with embodiment 1
The cell capture experiment of anticreep slide: taking the beaker of 15 50ml, be divided into 3 groups, often 5 beakers of group, are separately added into
Anticoagulated whole blood 100 μ l, then fill it up with non-ionic isotonic solution, mixing, wherein, first group of every beaker inserts at a piece of silane
The anticreep slide that reason agent (3-aminopropyl-(ethoxymethyl) silane) processes；Second group of every beaker inserts the left-handed bad ammonia of a piece of poly
The anticreep slide that acid (Poly-L-lysine) inorganic agent processes；3rd group of every beaker inserts a piece of embodiment of the present invention 1 make
The anticreep slide that the standby cation membranization dressing agent obtained processes.
On 20 DEG C of constant-temperature tables, with the speed jog 30 minutes of 40 beats/min, vertical take out anticreep slide, at microscope
Cell distribution homogeneity range is looked under high power lens, the cell number of 10 high power fields of counting, calculate average, its result such as table 1 below institute
Show:
1: three group of anticreep slide cell capture experimental result contrast table of table
As seen from Table 1, the cation membranization dressing agent of the present invention processes the anticreep slide obtained and the most common preventing
De-slide, its keyed jointing efficiency has significant difference, cell capture best results, provides optimum for later phase clinical pathological analysis
Specimen amount.
Claims (4)
1. a cation membranization dressing agent, it is characterised in that by weight, described cation membranization is modified
Agent includes following components:
A kind of cation membranization dressing agent the most according to claim 1, it is characterised in that by weight,
Described cation membranization dressing agent includes following components:
3. one kind utilize as arbitrary in claim 1 or 2 as described in cation membranization dressing agent prepare anticreep slide
Method, it is characterised in that comprise the following steps:
(1) clean alkalization slide is immersed process 2 minutes in coupling agent, in 37 DEG C of drying after vertical taking-up,
Obtain the slide of surface active；
(2) slide of the surface active of step (1) is vertically put in described cation membranization dressing agent and soak
10 minutes, in 37 DEG C of drying after vertical taking-up, it is thus achieved that described anticreep slide.
A kind of method utilizing cation membranization dressing agent to prepare anticreep slide the most according to claim 3,
It is characterized in that, the coupling agent of described step (1) is 3-aminopropyl-(ethoxymethyl) silane.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410443831.6A CN104176945B (en) | 2014-09-02 | 2014-09-02 | A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof |
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| CN201410443831.6A CN104176945B (en) | 2014-09-02 | 2014-09-02 | A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof |
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| CN104176945A CN104176945A (en) | 2014-12-03 |
| CN104176945B true CN104176945B (en) | 2016-08-17 |
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| CN110426257A (en) * | 2019-08-13 | 2019-11-08 | 南通美韦德生命科学有限公司 | A kind of adherency glass slide and preparation method thereof to fall off with high adhesive capacity preventing tissue |
Citations (3)
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| CN101122601A (en) * | 2007-09-24 | 2008-02-13 | 孙艳萍 | Method for separating and authenticating erythroblast of blood |
| CN103253873A (en) * | 2012-02-17 | 2013-08-21 | 麦克奥迪（厦门）医疗诊断系统有限公司 | Glass slide adhesive |
| CN103411818A (en) * | 2013-08-27 | 2013-11-27 | 苏州大猫单分子仪器研发有限公司 | Modifying glass slide for fixing biomacromolecules and method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1114909A (en) * | 1997-06-20 | 1999-01-22 | Bio Quest:Kk | Polymer coating slide glass |
| GB201004710D0 (en) * | 2010-03-22 | 2010-05-05 | Microsens Medtech Ltd | Detection of mycobacteria |
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2014
- 2014-09-02 CN CN201410443831.6A patent/CN104176945B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101122601A (en) * | 2007-09-24 | 2008-02-13 | 孙艳萍 | Method for separating and authenticating erythroblast of blood |
| CN103253873A (en) * | 2012-02-17 | 2013-08-21 | 麦克奥迪（厦门）医疗诊断系统有限公司 | Glass slide adhesive |
| CN103411818A (en) * | 2013-08-27 | 2013-11-27 | 苏州大猫单分子仪器研发有限公司 | Modifying glass slide for fixing biomacromolecules and method thereof |
Non-Patent Citations (1)
| Title |
|---|
| Mussel-Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization;Jungki Ryu et al.;《Advanced Functional Materials》;20100525;第20卷;第2132-2139页 * |
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Address after: 231400 No. 2 Dongyibei Road, Tongcheng Economic Development Zone, Anqing City, Anhui Province Patentee after: Anhui Xinling Laboratory Medicine Technology Co., Ltd. Address before: 231400 Tongcheng Economic Development Zone, Anqing City, Anhui Province Patentee before: Anhui Sinic Laboratory Medicine Technology Co., Ltd. |
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