CN104176945A - Cationic membranization modifier and preparation method of anti-falling slide using modifier - Google Patents
Cationic membranization modifier and preparation method of anti-falling slide using modifier Download PDFInfo
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- CN104176945A CN104176945A CN201410443831.6A CN201410443831A CN104176945A CN 104176945 A CN104176945 A CN 104176945A CN 201410443831 A CN201410443831 A CN 201410443831A CN 104176945 A CN104176945 A CN 104176945A
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Abstract
The invention discloses a cationic membranization modifier and a preparation method of an anti-falling slide using the modifier. The cationic membranization modifier comprises the following components in parts by weight: 1.5-2.5 parts of polydopamine, 0.5-1.5 parts of L-lysine, 1.5-2.5 parts of polyvinylpyrrolidone, 1.5-2.5 parts of polybrene, 0.5-1.5 parts of bovine blood albumin and 89.5-94.5 parts of ethanol. The preparation method comprises the following steps: treating a slide by a coupler at first and then treating the slide by the cationic membranization modifier to obtain the anti-falling slide. Compared with the prior art, the anti-falling slide has good cell-capture and anti-falling effects and provides optimal specimen amount for later-period clinical pathology analysis.
Description
Technical field
The present invention relates to be the check of a kind of clinical medicine time the Cell sheet glass of cell supporting body, the preparation method of a kind of positively charged ion membranization modifier and anticreep slide thereof in particular.
Background technology
Anticreep slide is again ion modification slide and cell capture slide etc., is with chemistry or physical method, the surface of slide glass or cover glass to be modified, to prevent that cell or tissue in operating process from falling the generation of sheet phenomenon.The application of anticreep slide is very extensive: as pathology tissue slice, cast-off cells film-making, and the liquid basal cell thin layer film-making in cell cultures, particularly cervical smear process etc.The use kind of anticreep slide is also various: as silex glass surface treated products such as positively charged ion anticreep slide, negatively charged ion anticreep slide, hydroformylation anticreep slide and silication anticreep slide etc.The treatment process of anticreep surface of glass slide also has multiple: as the ultrasonic wave of physical method, and the cleaning agent of chemical process or chemical modifier, or physical method and chemical process coupling.Wherein, the chemical modifier of use (tackiness agent) also has difference, as: poly-l-lysine (Poly-L-lysine), silane finish (3-aminopropyl-(ethoxymethyl) silane).But the two-dirnentional structure keyed jointing efficiency of above-mentioned this plane is lower, all can not solve sample flake problem completely.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, the preparation method of a kind of positively charged ion membranization modifier and anticreep slide thereof is provided, to solve the inefficient technical problem of anticreep slide anticreep under the effect of existing planar structural bond.
The present invention is achieved by the following technical solutions:
The invention provides a kind of positively charged ion membranization modifier, by weight, described positively charged ion membranization modifier comprises following component:
Take the component raw material of above-mentioned parts by weight, mix and ultrasonic dissolution assisting, until completely dissolved, the membrane filtration of 0.3 μ m, obtains described positively charged ion membranization modifier.
As a kind of preferred version, described positively charged ion membranization modifier by weight, comprises following component:
The present invention also provides a kind of anticreep slide that utilizes above-mentioned positively charged ion membranization modifier to prepare, and the preparation method of described anticreep slide, comprises the following steps:
(1) clean alkalization slide is immersed in coupling agent and processed 2 minutes, after vertical taking-up, in 37 ℃ of oven dry, obtain the slide of surface active;
(2) slide of the surface active of step (1) is vertically put into above-mentioned positively charged ion membranization modifier and soaked 10 minutes, after vertical taking-up, in 37 ℃ of oven dry, obtain described anticreep slide; Described anticreep slide aluminium film phonograph seal, preserves at 4～8 ℃.
The coupling agent of described step (1) is selected 3-aminopropyl-(ethoxymethyl) silane.
Principle of the present invention is: the present invention first by cationic polymers by surface-crosslinked technology, by the overlapping membranization of multiple cationic polymers group in surface of glass slide, make the surface of slide have a large amount of three-dimensional space cationic functional groups, positive charge and the corresponding function group acceptor of anticreep surface of glass slide have greatly been increased, in non-ionic damping fluid, the electronegative target cell of Clinical Laboratory can be adsorbed mutually with the positively charged ion membranization layer of this anticreep surface of glass slide, make the equally distributed cell thin of one deck, prevent cell detachment.
The present invention has the following advantages compared to existing technology: the preparation method who the invention provides a kind of positively charged ion membranization modifier and anticreep slide thereof, this anticreep slide is to utilize a large amount of three-dimensional space cationic functional groups that exist in its surface, adsorb mutually with electronegative target cell, its keyed jointing efficiency has significant difference than the common anticreep slide of existing market, its cell capture effect is good, for later stage Clinical and Pathological Analysis provides optimum specimen amount.
Embodiment
Below embodiments of the invention are elaborated, the present embodiment is implemented take technical solution of the present invention under prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
1, the preparation of positively charged ion membranization modifier:
By weight, take the component raw material of following umber:
By the above-mentioned raw material mixing taking ultrasonic, after solute dissolves completely, the membrane filtration of 0.3 μ m, obtains described positively charged ion membranization modifier.
2, the preparation of anticreep slide:
(1) clean alkalization slide is immersed in coupling agent 3-aminopropyl-(ethoxymethyl) silane and process 2 minutes, after vertical taking-up, in 37 ℃ of oven dry, obtain the slide of surface active;
(2) slide of the surface active of step (1) is vertically put into above-mentioned positively charged ion membranization modifier and soaked 10 minutes, after vertical taking-up, in 37 ℃ of oven dry, obtain described anticreep slide, by this anticreep slide aluminium film phonograph seal, save backup at 4～8 ℃.
Embodiment 2
The preparation method of the anticreep slide that the positively charged ion membranization modifier of the present embodiment is processed, wherein, described positively charged ion membranization modifier by weight, comprises following component:
Other steps are with embodiment 1.
Embodiment 3
The preparation method of the anticreep slide that the positively charged ion membranization modifier of the present embodiment is processed, wherein, described positively charged ion membranization modifier by weight, comprises following component:
Other steps are with embodiment 1
The cell capture experiment of anticreep slide: the beaker of getting 15 50ml, be divided into 3 groups, every group of 5 beakers, add respectively anticoagulated whole blood 100 μ l, fill it up with again non-ionic isotonic solution, mix, wherein, in first group of every beaker, insert the anticreep slide that a slice silane finish (3-aminopropyl-(ethoxymethyl) silane) is processed; In second group of every beaker, insert the anticreep slide that a slice poly-l-lysine (Poly-L-lysine) treatment agent is processed; In the 3rd group of every beaker, insert the anticreep slide that positively charged ion membranization modifier that a slice embodiment of the present invention 1 prepares is processed.
On 20 ℃ of constant-temperature tables, with the speed jogs of 40 beats/min 30 minutes, vertically take out anticreep slide, under microscope high power lens, look for cell distribution homogeneity range, the cell count of 10 high power fields of counting, computation of mean values, its result is as shown in table 1 below:
Table 1: three groups of anticreep slide cell capture experimental result contrast tables
As seen from Table 1, the anticreep slide that positively charged ion membranization modifier processing of the present invention obtains and common in the market anticreep slide, its keyed jointing efficiency has significant difference, and cell capture best results, for later stage Clinical and Pathological Analysis provides optimum specimen amount.
Claims (4)
1. a positively charged ion membranization modifier, is characterized in that, by weight, described positively charged ion membranization modifier comprises following component:
。
2. a kind of positively charged ion membranization modifier according to claim 1, is characterized in that, by weight, described positively charged ion membranization modifier comprises following component:
。
3. the positively charged ion membranization modifier of utilization as described in as arbitrary in claim 1 or 2 prepared a method for anticreep slide, it is characterized in that, comprises the following steps:
(1) clean alkalization slide is immersed in coupling agent and processed 2 minutes, after vertical taking-up, in 37 ℃ of oven dry, obtain the slide of surface active;
(2) slide of the surface active of step (1) is vertically put into described positively charged ion membranization modifier and soaked 10 minutes, after vertical taking-up, in 37 ℃ of oven dry, obtain described anticreep slide.
4. a kind of method of utilizing positively charged ion membranization modifier to prepare anticreep slide according to claim 3, is characterized in that, the coupling agent of described step (1) is 3-aminopropyl-(ethoxymethyl) silane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410443831.6A CN104176945B (en) | 2014-09-02 | 2014-09-02 | A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410443831.6A CN104176945B (en) | 2014-09-02 | 2014-09-02 | A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104176945A true CN104176945A (en) | 2014-12-03 |
| CN104176945B CN104176945B (en) | 2016-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410443831.6A Active CN104176945B (en) | 2014-09-02 | 2014-09-02 | A kind of cation membranization dressing agent and the preparation method of anticreep slide thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110426257A (en) * | 2019-08-13 | 2019-11-08 | 南通美韦德生命科学有限公司 | A kind of adherency glass slide and preparation method thereof to fall off with high adhesive capacity preventing tissue |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1114909A (en) * | 1997-06-20 | 1999-01-22 | Bio Quest:Kk | Polymer coating slide glass |
| CN101122601A (en) * | 2007-09-24 | 2008-02-13 | 孙艳萍 | Method for separating and authenticating erythroblast of blood |
| WO2011117201A1 (en) * | 2010-03-22 | 2011-09-29 | Microsens Medtech Limited | Detection of mycobacteria |
| CN103253873A (en) * | 2012-02-17 | 2013-08-21 | 麦克奥迪（厦门）医疗诊断系统有限公司 | Glass slide adhesive |
| CN103411818A (en) * | 2013-08-27 | 2013-11-27 | 苏州大猫单分子仪器研发有限公司 | Modifying glass slide for fixing biomacromolecules and method thereof |
-
2014
- 2014-09-02 CN CN201410443831.6A patent/CN104176945B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1114909A (en) * | 1997-06-20 | 1999-01-22 | Bio Quest:Kk | Polymer coating slide glass |
| CN101122601A (en) * | 2007-09-24 | 2008-02-13 | 孙艳萍 | Method for separating and authenticating erythroblast of blood |
| WO2011117201A1 (en) * | 2010-03-22 | 2011-09-29 | Microsens Medtech Limited | Detection of mycobacteria |
| CN103253873A (en) * | 2012-02-17 | 2013-08-21 | 麦克奥迪（厦门）医疗诊断系统有限公司 | Glass slide adhesive |
| CN103411818A (en) * | 2013-08-27 | 2013-11-27 | 苏州大猫单分子仪器研发有限公司 | Modifying glass slide for fixing biomacromolecules and method thereof |
Non-Patent Citations (1)
| Title |
|---|
| JUNGKI RYU ET AL.: "Mussel-Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization", 《ADVANCED FUNCTIONAL MATERIALS》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110426257A (en) * | 2019-08-13 | 2019-11-08 | 南通美韦德生命科学有限公司 | A kind of adherency glass slide and preparation method thereof to fall off with high adhesive capacity preventing tissue |
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| CN104176945B (en) | 2016-08-17 |
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Address after: 231400 No. 2 Dongyibei Road, Tongcheng Economic Development Zone, Anqing City, Anhui Province Patentee after: Anhui Xinling Laboratory Medicine Technology Co., Ltd. Address before: 231400 Tongcheng Economic Development Zone, Anqing City, Anhui Province Patentee before: Anhui Sinic Laboratory Medicine Technology Co., Ltd. |
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