CN104496724B - Organic fungi-manure for manganese ore contaminated soil remediation and preparation method thereof - Google Patents
Organic fungi-manure for manganese ore contaminated soil remediation and preparation method thereof Download PDFInfo
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- CN104496724B CN104496724B CN201410809874.1A CN201410809874A CN104496724B CN 104496724 B CN104496724 B CN 104496724B CN 201410809874 A CN201410809874 A CN 201410809874A CN 104496724 B CN104496724 B CN 104496724B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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Abstract
The invention discloses a kind of organic fungi-manure for manganese ore contaminated soil remediation and preparation method thereof, wherein organic fungi-manure include the anti-manganese complex micro organism fungicide of the palliative compound Organic Ingredientss, the water conservation medium of 70～75 mass parts and 3～5 mass parts of the basic nutrient solution of 3～5 mass parts, 20～25 mass parts.Its preparation method includes mixing and fermentation step.The organic fungi-manure for manganese ore contaminated soil remediation of the present invention, the effects such as having improved soil structure, water holding constant temperature, reduce Heavy Metals In The Rhizosphere toxicity, improve plant survival rate, promote plant growing；Furthermore it is also possible to effectively alleviate plant rhizosphere soil manganese element toxicity, improve fertilizer effect, fortification of plants repair ability.
Description
Technical field
The present invention relates to technical field of fertilizers, more particularly, to a kind of organic fungi-manure for manganese ore contaminated soil remediation, also
It is related to the preparation method of this organic fungi-manure.
Background technology
China's manganese ore type is many, distribution is wide, is usually polynary heavy metal by the soil pollution that mining smelting activity produces multiple
Close pollution, main pollution-producing is Mn, Pb, Zn, Cd etc..
Aheap soil vegetative cover destroys serious, moisture, nutrient substance deficiency to manganese mud, and heavy metal toxicity is too high, so that big
Most microorganisms and floristics are difficult to survive in the environment, therefore become the main limit of heavy-metal contaminated soil vegetation repair
The factor processed.Due in the soil type of different mining areas heavy metal element species and pollution level different it is therefore desirable to for not
With contaminated soil characteristic, invention one kind can effectively reduce plant rhizosphere soil toxicity, improve the special of plant root growth environment
Fertile.Additionally, in south China mining-area plant is repaired, moisture condition is typically plant survival and growth limiting factor, and manganese ore
Slag not only fertilizer deficiency, water retention property also extreme difference, it is therefore desirable to special fertilizer has fertilizer, water conservation and alleviates heavy metal toxicity simultaneously
Complex function.
But it is not a kind of at present true according to manganese ore Heavy Metals in Contaminated Soils pollutant composition and its pollution level
Determine the special organic fungi-manure of formula, therefore a kind of to can be applicable to different manganese ore contaminated areas phytoremediation special in the urgent need to inventing
Organic fungi-manure, special organic fungi-manure requires to have to improve contaminated area plant root growth environmental functional, can provide for plant growing
Necessary nutrient, strengthens the water holding constant temperature ability of rhizosphere soil and effective toxicity reducing Heavy Metal Speciation in Rhizosphere.
Content of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, provides one kind to have water holding water conservation, improvement
The effects such as soil, culture fertility；Plant rhizosphere soil manganese element toxicity can also effectively be alleviated, improve fertilizer effect, strengthening
The organic fungi-manure for manganese ore contaminated soil remediation of plant remediation ability, additionally provides the preparation method of organic fungi-manure.
For solving above-mentioned technical problem, there is provided a kind of organic fungi-manure for manganese ore contaminated soil remediation, including 3～5
The basic nutrient solution of mass parts, the palliative of 20～25 mass parts are combined Organic Ingredientss, the water conservation medium and 3 of 70～75 mass parts
The anti-manganese complex micro organism fungicide of～5 mass parts.
Above-mentioned organic fungi-manure is it is preferred that anti-manganese complex micro organism fungicide includes bacillus megaterium microbial inoculum, produces sour Cray
Bai Shi bacillus microbial inoculum and citrobacter freundii microbial inoculum, the sour klebsiella microbial inoculum of bacillus megaterium microbial inoculum, product and Freund
The mass ratio of citric acid bacillus microbial inoculum is 2: 1: 2.
Above-mentioned organic fungi-manure is it is preferred that the preparation method of bacillus megaterium microbial inoculum comprises the following steps：
(1) domestication culture：Bacillus megaterium is tamed shaking table culture 2～3 in fluid medium in bacillus megaterium
My god, then proceed to culture in bacillus megaterium domestication solid medium and obtain bacterium colony；
(2) continuous domestication：Picking colony continues, according to step (1) domestication and culture method continuous domestication 3～5 times, to be followed by most
Plant and obtain bacillus megaterium microbial inoculum in bacillus megaterium enrichment medium.
Above-mentioned organic fungi-manure is it is preferred that the formula that bacillus megaterium tames fluid medium is：Carnis Bovis seu Bubali cream 3g/L,
Peptone 10g/L, NaCl 5g/L, MnSO4400mg/L, pH regulator to 5.
Above-mentioned organic fungi-manure is it is preferred that the preparation method producing sour klebsiella microbial inoculum comprises the following steps：
(3) domestication culture：Sour klebsiella shaking table in producing sour klebsiella domestication fluid medium will be produced
Culture 2～3 days, then proceeds to culture in product sour klebsiella domestication solid medium and obtains bacterium colony；
(4) continuous domestication：Picking colony continues, according to step (3) domestication and culture method continuous domestication 3～5 times, to be followed by most
Plant to produce in sour klebsiella enrichment medium and obtain producing sour klebsiella microbial inoculum.
Above-mentioned organic fungi-manure is it is preferred that product sour klebsiella domestication liquid culture medium prescription is：Carnis Bovis seu Bubali cream 3g/
L, peptone 10g/L, NaCl 5g/L, MnSO4400mg/L, pH regulator to 8.
Above-mentioned organic fungi-manure is it is preferred that the preparation method of citrobacter freundii microbial inoculum comprises the following steps：
(5) domestication culture：Citrobacter freundii is tamed shaking table culture 2 in fluid medium in citrobacter freundii
～3 days, then proceed to culture in citrobacter freundii domestication solid medium and obtain bacterium colony；
(6) continuous domestication：Picking colony continues, according to step (5) domestication and culture method continuous domestication 3～5 times, to be followed by most
Plant and obtain citrobacter freundii microbial inoculum in citrobacter freundii enrichment medium.
Above-mentioned organic fungi-manure is it is preferred that citrobacter freundii domestication liquid culture medium prescription is：Carnis Bovis seu Bubali cream 3g/L,
Peptone 10g/L, NaCl 5g/L, MnSO4400mg/L, pH regulator to 6.5.
Above-mentioned organic fungi-manure is it is preferred that basic nutrient solution includes the calcium superphosphate, sulphuric acid that mass ratio is 4: 3: 7: 5: 1
Potassium, sodium hydroxide, disodium hydrogen phosphate and sodium dihydrogen phosphate；Palliative is combined Organic Ingredientss and is pressed by cattle manure, chicken manure and rice straw
Prepare than for carrying out fermentation after 3: 2: 1 mixing according to weight；Water conservation medium is peat soil and/or plant ash.
As a total technology design, present invention also offers the preparation method of above-mentioned organic fungi-manure, including following
Step：Basic nutrient solution, palliative are combined after Organic Ingredientss and water conservation medium mix homogeneously, add anti-manganese complex microorganism
Microbial inoculum, then in 30 DEG C of bottom fermentations 15 days, completes the preparation of the organic fungi-manure for manganese ore contaminated soil remediation.
Compared with prior art, it is an advantage of the current invention that：
(1) present invention, with organic media and water conservation medium as main component, is being combined using basic nutrient solution and palliative
Organic Ingredientss meet on the basis of plant nutrient demand in contaminated soil in mining areas, with the addition of domestication under high concentration heavy metal environment
Afterwards there is toleration and remove the strong anti-manganese complex micro organism fungicide of removing heavy metals ability, in order to reduce Index of Heavy Metal Toxicity in Soil,
The fertilizer efficiency of strengthening organic fungi-manure.
Wherein basic nutrient solution is nutrient substance necessary to plant growing, can supplement rapidly basic needed for plant growing
Nutrition；Palliative is combined Organic Ingredientss one side can increase the organic matter of soil, and the growth for plant provides nutrient,
Ensure that the survival of the sour klebsiella of bacillus megaterium, product and citrobacter freundii, another aspect palliative is combined
Organic Ingredientss can reduce the contents level of heavy metals in soil by the effect such as absorption, oxidoreduction, complexation, thus reducing
The toxicity of root system of plant area heavy metal, promotes plant normal growth；Water conservation medium has absorption and water retention property, and it can pass through
Adsorption reduces the toxicity of Heavy Metal Speciation in Rhizosphere element, the effect having moisturizing simultaneously and adjusting the soil moisture, therefore may be used
Alleviating arid and high temperature affects on the stress of plant；Anti- manganese complex micro organism fungicide heavy metal has absorption, conversion, absorbs and profit
Effect, can effectively reduce the activity of heavy metal in soil.
(2) bacillus megaterium, the sour klebsiella of product and citrobacter freundii are carried out domestication culture by the present invention
After be prepared into microbial inoculum, through domestication after bacterial strain there is very strong anti-manganese performance, have certain absorption Absorption to manganese simultaneously, join
Close the compound Organic Ingredientss of palliative and be applied to the content that can effectively reduce Manganese In Soils in contaminated soil, thus playing Polluted Soil
The effect that earth is repaired.
(4) organic fungi-manure for manganese ore contaminated soil remediation of the present invention can significantly improve manganese ore contaminated area rehabilitation plant
The growing environment of root system, therefore can play an important role in the restoration of the ecosystem of south China manganese ore contaminated area.
(5) the invention provides a kind of preparation method of the organic fungi-manure for manganese ore contaminated soil remediation, there is preparation
Process is simple, the low advantage of production cost, it is suitable for and large-scale industrial production.
Specific embodiment
Below in conjunction with concrete preferred embodiment, the invention will be further described, but not thereby limiting the invention
Protection domain.
Embodiment
Material employed in following examples and instrument are commercially available.Wherein bacillus megaterium, Freund citric acid bar
Bacterium and aspergillus oryzae all screen from Hunan Zixing Manganese Ore District soil, but its colonial morphology, inherited character, effect etc. are huge with commercially available
The sour klebsiella of Bacterium anthracoides, citrobacter freundii, product is consistent.Commercially available bacillus megaterium, Freund citric acid
The sour klebsiella of bacillus, product equally can prepare the organic fungi-manure for manganese ore contaminated soil remediation of the present invention, and reaches
Same or analogous technique effect.
Embodiment 1：
A kind of organic fungi-manure for manganese ore contaminated soil remediation of present invention, through to trial zone heavy metal content in soil
Measure, determine that the formula of special organic fungi-manure is：The basic nutrient solution of 5 mass parts, the palliative of 20 mass parts are combined organic former
Material, the water conservation medium (the water conservation medium that the present embodiment adopts is that peat soil adds plant ash) of 70 mass parts and the anti-manganese of 5 mass parts
Ore deposit complex micro organism fungicide.
It is used for the preparation method of the organic fungi-manure of manganese ore contaminated soil remediation in previous embodiment 1, specifically include following step
Suddenly：
(1) prepare basic nutrient solution：Calcium superphosphate, potassium sulfate, sodium hydroxide, disodium hydrogen phosphate and sodium dihydrogen phosphate are mixed
Conjunction obtains basic nutrient solution；In basic nutrient solution, calcium superphosphate, potassium sulfate, sodium hydroxide, disodium hydrogen phosphate and sodium dihydrogen phosphate
Mass ratio be 4: 3: 7: 5: 1.
(2) prepare palliative and be combined Organic Ingredientss：Cattle manure, chicken manure and rice straw are mixed for 3: 2: 1 according to mass ratio
Obtain mixture, mixture is stacked at 20 days (fermentation time all can be implemented for 15～20 days) of fermentation in 35 DEG C of room temperatures, previous
Every turning in 2 days once turning in 5 days afterwards once, obtained palliative and was combined Organic Ingredientss week.
(3) tame bacillus megaterium：
3.1 domestication cultures：Bacillus megaterium is shaken for 28 DEG C～35 DEG C in bacillus megaterium domestication fluid medium
Bed culture obtains bacterium solution (shaking table culture all can be implemented for 2～3 days) for 3 days, and picking bacterium solution is placed in bacillus megaterium domestication solid training
, bacterium colony in bacillus megaterium domestication solid medium in culture 3 days (culture all can be implemented for 3～5 days) in foster base.Huge
Bacterium anthracoides tame fluid medium formula be：By the peptone of the Carnis Bovis seu Bubali cream of 3g, 10g, the NaCl of 5g is dissolved in 950mL and surpasses
In pure water, it is subsequently adding MnSO4Mother solution is simultaneously settled to 1000mL and obtains mixed solution；MnSO in aforementioned mixed solution4Concentration
400mg/L, pH regulator to 5.The formula that bacillus megaterium tames solid medium is in bacillus megaterium domestication liquid training
20g/L agar (adding 15～20g/L agar in formula) is added in the formula of foster base.
3.2 continuous domestication：Bacterium colony in picking solid medium continues to be inoculated in bacillus megaterium domestication liquid culture
In base, repeat 3.1 steps 5 time (repeat tame 3～5 times all can implement).
3.3 preparation bacillus megaterium microbial inoculums：Picking bacillus megaterium tames the colony inoculation of solid medium to huge
Bacterium colony is obtained, then the bacterium colony in scraping bacillus megaterium solids enrichment culture medium in Bacterium anthracoides solids enrichment culture medium
It is configured to bacillus megaterium microbial inoculum.The formula of bacillus megaterium solids enrichment culture medium is solid with bacillus megaterium domestication
The formula of body culture medium is identical.
(4) sour klebsiella is produced in domestication：
4.1 domestication cultures：Sour klebsiella will be produced 28 DEG C in producing sour klebsiella domestication fluid medium
～35 DEG C of shaking table cultures 2 obtain bacterium solution (shaking table culture all can be implemented for 2～3 days), and picking bacterium solution is placed in the sour klebsiella of product
Culture 4 days (culture all can be implemented for 3～5 days) in domestication solid medium, until producing sour klebsiella domestication solid culture
Bacterium colony occurs in base.Produce sour klebsiella and tame the formula of fluid medium and be：By the peptone of the Carnis Bovis seu Bubali cream of 3g, 10g,
The NaCl of 5g is dissolved in 950mL ultra-pure water, is subsequently adding MnSO4Mother solution is simultaneously settled to 1000mL and obtains mixed solution；Aforementioned mixed
Close MnSO in solution4Concentration 400mg/L, pH regulator to 8.Produce sour klebsiella and tame the formula of solid medium for producing acid
The formula that klebsiella tames solid medium is to add in the formula producing sour klebsiella domestication fluid medium
Enter 20g/L agar (adding 15～20g/L agar in formula).
4.2 continuous domestication：The bacterium colony that picking produces in sour klebsiella domestication solid medium continues to be inoculated in product acid
In klebsiella domestication fluid medium, repeat 4.1 steps 4 time (repeat tame 3～5 times all can implement).
Sour klebsiella microbial inoculum is produced in 4.3 preparations：Picking produces the bacterium colony that sour klebsiella tames solid medium
It is inoculated in the sour klebsiella solids enrichment culture medium of product and obtains bacterium colony, then the sour klebsiella solid of scraping product is rich
Bacterium colony in collection culture medium is configured to produce sour klebsiella microbial inoculum.Produce joining of sour klebsiella solids enrichment culture medium
Side is identical with the formula producing sour klebsiella domestication solid medium.
(5) tame citrobacter freundii：
5.1 domestication cultures：Citrobacter freundii is tamed shaking table culture 3 in fluid medium in citrobacter freundii
It obtains bacterium solution (shaking table culture all can be implemented for 2～3 days), and picking bacterium solution is placed in citrobacter freundii domestication solid medium
, bacterium colony in citrobacter freundii domestication solid medium in culture 3 days (culture all can be implemented for 3～5 days).Freund lemon
Lemon acidfast bacilli tame fluid medium formula be：By the peptone of the Carnis Bovis seu Bubali cream of 3g, 10g, it is ultrapure that the NaCl of 5g is dissolved in 950mL
In water；It is subsequently adding MnSO4Mother solution, and be settled to 1000mL and obtain mixed solution；MnSO in aforementioned mixed solution4Concentration
400mg/L, pH regulator to 6.5.The formula that citrobacter freundii tames solid medium is in citrobacter freundii domestication
20g/L agar (adding 15～20g/L agar in formula) is added in the formula of fluid medium.
5.2 continuous domestication：The bacterium colony that picking citrobacter freundii is tamed in solid medium continues to be inoculated in Freund lemon
In lemon acidfast bacilli domestication fluid medium, repeat 5.1 steps 4 time (repeat tame 3～5 times all can implement).
5.3 prepare citrobacter freundii microbial inoculum：Picking citrobacter freundii tames the colony inoculation of solid medium
Obtain bacterium colony in citrobacter freundii solids enrichment culture medium, then scrape citrobacter freundii solids enrichment culture medium
In bacterium colony be configured to citrobacter freundii microbial inoculum.The formula of citrobacter freundii solids enrichment culture medium is and Freund lemon
The formula that lemon acidfast bacilli tames solid medium is identical.
(6) prepare anti-manganese complex micro organism fungicide：Bacillus megaterium microbial inoculum that step (3) is prepared, step (4)
The citrobacter freundii microbial inoculum that product acid klebsiella microbial inoculum, the step (5) preparing prepares is according to concentration ratio 2
: 1: 2 mixing, obtain anti-manganese complex micro organism fungicide, in microbial inoculum, viable bacteria content reaches 6 × 1010Individual/more than mL, wherein huge bud
The content of spore bacillus microbial inoculum is 2.4 × 1010Individual/mL, the content producing sour klebsiella is 1.2 × 1010Individual/mL, Freund lemon
The content of lemon acidfast bacilli is 2.4 × 1010Individual/mL.
(6) compost：Basic nutrient solution, palliative are combined after Organic Ingredientss and water conservation medium mix homogeneously, add anti-
Manganese complex micro organism fungicide, then in 30 DEG C of bottom fermentations 15 days, completes the system of the organic fungi-manure for manganese ore contaminated soil remediation
Standby.
Embodiment 1 is only the preferred embodiments of the present invention, and in the present invention, the formula of organic fungi-manure is：3～5 mass parts
Basic nutrient solution, the palliative of 20～25 mass parts be combined Organic Ingredientss, the water conservation medium of 70～75 mass parts and 3～5 matter
The anti-manganese complex micro organism fungicide of amount part, all can implement, and reach same as Example 1 or similar technique effect.
Embodiment 2：
A kind of organic fungi-manure for manganese ore contaminated soil remediation of embodiment 1 answering in repairing manganese ore contaminated soil
With pot experiment is carried out as substrate using the slag gathering from Hunan Zixing manganese Tailings Dam.
Experimental group：The organic fungi-manure of embodiment 1 is mixed dress basin with the slag of manganese Tailings Dam according to mass ratio 1: 4 is uniform.
Set 8 basin Duplicate Samples altogether.
Matched group：Add N, P, K chemical fertilizer with the organic fungi-manure moderate of embodiment 1 in the slag of manganese mine tailing, and fill
Basin.Set 8 basin Duplicate Samples altogether.
The plant nursery stock of three plants of screenings transplanted by every basin.Test plant plant height and the increment of Biomass are investigated in plantation after 1 year
Change, the results are shown in Table 1 for investigation.
Table 1：Pot experiment plant growing increment
Knowable to the investigation result of table 1：After plantation 1 year, the plant survival rate of experimental group is all higher than 88%, viable plant
Well-grown；Control group plants survival rate is less than 45%, and viable plant leaf is yellow, and growth is almost stagnated.
Embodiment 3：The organic fungi-manure being used for manganese ore contaminated soil remediation of embodiment 1 is used for manganese tailings pollution area ecological
Recovery project.
On Hunan Zixing manganese ore mine tailings ground, establish 4hm2Manganese ore waste residue ground restoration of the ecosystem demonstration project, before reparation
Phase, measure analysis project area slag sample, slag ground nutrient levels, pollution element species and its pollution level, cut open in 0～50cm
In face, the content of soil Mn, Pb, Zn, Cd element is respectively 12251mg/Kg, 37301mg/Kg, 4304mg/Kg, 100mg/Kg,
Far above Hunan Province and Chinese background value；Belong to multi-metal elements compounding type serious pollution.
Test block：Mix plantation Paulownia and Luan Shu in this demonstration area, method of forestation is to dig cave 0.5m × 0.5m × 0.5m, so
Add the organic fungi-manure for repairing anti-soil polluted by manganese of embodiment 1 afterwards in cave, make organic fungi-manure and slag substrate in cave
It is 1: 4 mix homogeneously according to mass ratio, then transplant annual xylophyta nursery stock.
Check plot：Equally afforest in an identical manner, apply the chemical fertilizer of N, P, K equivalent in the organic fungi-manure with embodiment 1.
Investigate the growing state of various plants and existing state in test block and check plot, table 2 is the woody plant of test block
Thing growth conditions.
Table 2：Ecological Restoration Project area vegetation growth state
After the afforestation of test block, the survival rate of First Year Paulownia and Luan Shu is respectively 83.3% and 86.7%, and next year reseeds, and plants
To 100%, upgrowth situation is good for thing survival rate.Check plot is applied with the chemical fertilizer with N, P, K equivalent in special organic fungi-manure, plants
After plant, the survival rate of First Year Paulownia and Luan Shu is respectively 15% and 21%, and next year does not reseed, and survival rate is reduced to 10%
With 11%, the survival rate of the 3rd year two seeds is respectively less than 5%, and viable plant leaf is yellow, and growth is almost stagnated.
The above, be only presently preferred embodiments of the present invention, and not the present invention is made with any pro forma restriction.Though
So the present invention is disclosed as above with preferred embodiment, but is not limited to the present invention.Any it is familiar with those skilled in the art
Member, in the case of the spirit without departing from the present invention and technical scheme, can be utilized in the methods and techniques of the disclosure above
Hold and technical solution of the present invention is made with many possible variations and modification, or the Equivalent embodiments being revised as equivalent variations.Therefore,
Every content without departing from technical solution of the present invention, the technical spirit of the foundation present invention is to made for any of the above embodiments any simple
Modification, equivalent, equivalence changes and modification, all still fall within the range of technical solution of the present invention protection.
Claims (9)
1. be used for manganese ore contaminated soil remediation organic fungi-manure it is characterised in that include 3～5 mass parts basic nutrient solution, 20
The anti-manganese of the palliative of～25 mass parts compound Organic Ingredientss, the water conservation medium of 70～75 mass parts and 3～5 mass parts is combined micro-
Bacteria agent；Described anti-manganese complex micro organism fungicide include bacillus megaterium microbial inoculum, produce sour klebsiella microbial inoculum and
Citrobacter freundii microbial inoculum, the sour klebsiella microbial inoculum of described bacillus megaterium microbial inoculum, product and citrobacter freundii
The mass ratio of microbial inoculum is 2: 1: 2.
2. organic fungi-manure according to claim 1 is it is characterised in that the preparation method bag of described bacillus megaterium microbial inoculum
Include following steps：
（1）Domestication culture：Bacillus megaterium is tamed shaking table culture 2～3 days in fluid medium in bacillus megaterium, so
After proceed to bacillus megaterium domestication solid medium in culture obtain bacterium colony；
（2）Continuous domestication：Bacterium colony described in picking continues according to step（1）Described domestication and culture method continuous domestication 3～5 times,
After be inoculated in bacillus megaterium enrichment medium and obtain bacillus megaterium microbial inoculum.
3. organic fungi-manure according to claim 2 is it is characterised in that described bacillus megaterium tames fluid medium
Formula is：Carnis Bovis seu Bubali cream 3 g/L, peptone 10 g/L, NaCl 5 g/L, MnSO4400mg/L, pH regulator to 5.
4. organic fungi-manure according to claim 1 it is characterised in that described product acid klebsiella microbial inoculum preparation side
Method comprises the following steps：
（3）Domestication culture：Sour klebsiella shaking table culture 2 in producing sour klebsiella domestication fluid medium will be produced
～3 days, then proceed to culture in product sour klebsiella domestication solid medium and obtain bacterium colony；
（4）Continuous domestication：Bacterium colony described in picking continues according to step（3）Described domestication and culture method continuous domestication 3～5 times,
After be inoculated in the sour klebsiella enrichment medium of product and obtain producing sour klebsiella microbial inoculum.
5. organic fungi-manure according to claim 4 is it is characterised in that described product acid klebsiella tames liquid culture
Based formulas are：Carnis Bovis seu Bubali cream 3 g/L, peptone 10 g/L, NaCl 5 g/L, MnSO4400mg/L, pH regulator to 8.
6. organic fungi-manure according to claim 1 is it is characterised in that the preparation method of described citrobacter freundii microbial inoculum
Comprise the following steps：
（5）Domestication culture：Citrobacter freundii is tamed shaking table culture 2～3 in fluid medium in citrobacter freundii
My god, then proceed to culture in citrobacter freundii domestication solid medium and obtain bacterium colony；
（6）Continuous domestication：Bacterium colony described in picking continues according to step（5）Described domestication and culture method continuous domestication 3～5 times,
After be inoculated in citrobacter freundii enrichment medium and obtain citrobacter freundii microbial inoculum.
7. organic fungi-manure according to claim 6 is it is characterised in that described citrobacter freundii tames fluid medium
Formula is：Carnis Bovis seu Bubali cream 3 g/L, peptone 10 g/L, NaCl 5 g/L, MnSO4400mg/L, pH regulator to 6.5.
8. organic fungi-manure according to any one of claim 1 to 7 is it is characterised in that described basic nutrient solution includes
Mass ratio is 4: 3: 7: 5: 1 calcium superphosphate, potassium sulfate, sodium hydroxide, disodium hydrogen phosphate and sodium dihydrogen phosphate；Described alleviation
Property compound Organic Ingredientss be to carry out fermentation after 3: 2: 1 mixing to prepare by cattle manure, chicken manure and rice straw according to mass ratio；Institute
The water conservation medium stated is peat soil and/or plant ash.
9. a kind of preparation method of organic fungi-manure any one of claim 1 to 8 is it is characterised in that comprise the following steps：
Basic nutrient solution, palliative are combined after Organic Ingredientss and water conservation medium mix homogeneously, add anti-manganese complex micro organism fungicide,
Then in 30 DEG C of bottom fermentations 15 days, complete the preparation of the organic fungi-manure for manganese ore contaminated soil remediation.
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| CN106554238A (en) * | 2016-11-23 | 2017-04-05 | 广东中联兴环保科技有限公司 | A kind of modifying agent in Rare Earth Mine tailings slash and its multiple method for green of vegetation |
| CN109777839B (en) * | 2017-11-10 | 2021-05-14 | 东北林业大学 | Method for removing cadmium in water and generating nano material by using citrobacter freundii |
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