CN104491289A - Traditional Chinese medicine preparation for treating amenorrhoea, as well as preparation method thereof - Google Patents
Traditional Chinese medicine preparation for treating amenorrhoea, as well as preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine preparation for treating amenorrhoea, belonging to the technical field of traditional Chinese medicines. The traditional Chinese medicine preparation is prepared from the following bulk pharmaceutical chemicals: radix codonopsis, rhizoma atractylodis macrocephalae, ramulus cinnamomi, poria, semen persicae, radix rehmanniae praeparata, radix paeoniae alba, spindle tree wings, radix angelicae sinensis, colla corii asini, cortex moutan radicis, radix salviae miltiorrhizae, eupolyphaga seu steleophaga, hirudo, fructus liquidambaris, rhizoma cyperi, whole scorpio, mallow seeds, radix glycyrrhizae and the like. The preparation prepared from the traditional Chinese medicine materials has the therapeutic advantages of being fast to effect, obvious in efficacy, treating both manifestation and root cause of disease, conditioning and nourishing, and being free from toxic and side effects and the like when being used for treating amenorrhoea, and for the preparation, the preparation technology is simple and convenient as well as easy, the efficacy is stable, individual difference is small, and the preparation is easy to popularize and apply.
Description
Technical field
The present invention relates to technical field of Chinese medicines, particularly relate to a kind of Chinese medicine preparation for the treatment of amenorrhea.
Technical background
Amenorrhea is symptom common in gynaecopathia, can be caused by various different reason.Usually amenorrhea is divided into constitutional and Secondary cases two kinds.All persons that do not pass through yet over 18 years old are called primary amenorrhea; After menarche, in any time before normal menopause (gestation or age of sucking except), menstruation apolipsis is called secondary amenorrhea more than 6 months persons.Such differentiation is artificial to a great extent, because the Fundamentals causing former and secondary amenorrhea may be identical sometimes.But when providing the clue of the cause of disease and prognosis, this division is valuable, such as most birth defect, comprise the abnormal development of ovary or Seedling Le Shi tissue, the amenorrhea caused is put into primary amenorrhea, and secondary amenorrhea majority is caused by acquired disease, and more easily treats.
Mostly the treatment means of doctor trained in Western medicine is the mode adopting hormone therapy, this mode weak effect, take effect slow, side effect is large.
Amenorrhea is the commonly encountered diseases of gynecological, and the discussion of the traditional Chinese medical science to amenorrhea is a lot of, and Chinese traditional treatment amenorrhea mainly adopts the method for Coryza Treated by Syndrome Differentiation to carry out, and qi stagnation and blood stasis type, weak type of qi and blood, kidney qi deficiency, type of stagnation of phlegm-damp, type of YIN-deficiency and blood-dryness can be divided into treat.The Syndrome Differentiation of Traditional Chinese Medicine of amenorrhea specifically has following several:
1, qi stagnation and blood stasis type
Main symptom: the menstruation several months is not all right, spirit depressing, or fidgety irritability, fullness and distention in the chest and hypochondrium, breast, lower abdominal distention pain, dimly red tongue, has petechia ecchymosis, stringy and hesitant pulse.Method for the treatment of: vital energy regualting and blood circulation-promoting, blood stasis dispelling stimulates the menstrual flow.
2, weak type of qi and blood
Main symptom: menstruation is delayed gradually, amount is few, and the light matter of color is rare, then closes down, shallow complexion, and hair is not damp or come off, and feels dizzy, shortness of breath and palpitation, fatigue in both spirit and physical strength, inappetence, pale tongue, white and thin fur, deficient and thready pulse.Method for the treatment of: benefiting qi and nourishing blood regulating menstruation.
3, kidney qi deficiency
Cure mainly: woman is not yet risen over the menstruation of 18 one full year of life, or delayed menstrual cycle oligomenorrhea gradually turning into amenorrhea, dim complexion, alopecia, hyposexuality, dizziness and tinnitus, soreness of the waist and knees, clear urine in large amounts, frequent urination at night, large loose stool, pale tongue, tongue is white, deep and weak pulse.Method for the treatment of: the kidney invigorating and essence nourishing regulating menstruation.
4, type of stagnation of phlegm-damp
Main symptom: amenorrhea, obesity, fullness in the chest and hypochondrium is vexed, vomiting and nausea abundant expectoration, and profuse leukorrhea color is white, and body of the tongue is light red, and tongue is white thick greasy, rolling pulse.Method for the treatment of: drying dampness to eliminate phlegm, promoting blood circulation to restore menstrual flow.
5, type of YIN-deficiency and blood-dryness
Main symptom: menses are by few gradually to closing down, and hectically flushed cheeks, dysphoria with feverish sensation in the chest palms and soles, feverish sensation in the palms and soles, hectic fever in the afternoon, body is very thin, and body of the tongue is light red, few tongue, thready and rapid pulse.Method for the treatment of: replenishing YIN and removing heat regulating menstruation.
Summary of the invention
The technical problem to be solved in the present invention is a kind of Chinese medicine for the treatment of amenorrhea of exploitation, and the clinical practice feature adapting to modern is made and facilitated finished product preparation that is easy-to-use, stable curative effect.
In order to solve the problem, the technical scheme of inventor is, the feature of preparation process and Traditional Chinese Medicine is extracted to the dialectical cognition of amenorrhea in conjunction with modern Chinese medicine, again prescription, and Clinical Practice adjustment checking, the Chinese prescription that final formation one is brand-new and brand-new preparation technology corresponding with it, the Chinese medicine preparation made by this prescription and preparation technology has very significant therapeutic effect to amenorrhea, this Chinese prescription is made up of the Chinese crude drug of following weight portion: Radix Codonopsis 16-30 part, Rhizoma Atractylodis Macrocephalae 15-29 part, Ramulus Cinnamomi 15-25 part, Poria 8-22 part, Semen Persicae 10-24 part, Radix Rehmanniae Preparata 7-21 part, Radix Paeoniae Alba 17-31 part, Ramulus Euonymi 6-14 part, Radix Angelicae Sinensis 8-22 part, Colla Corii Asini 9-19 part, Cortex Moutan 7-17 part, Radix Salviae Miltiorrhizae 6-16 part, Eupolyphaga Seu Steleophaga 3-7 part, Hirudo 3-7 part, Fructus Liquidambaris 8-18 part, Rhizoma Cyperi 6-14 part, Scorpio 2-6 part, Semen Abutili 11-21 part, Radix Glycyrrhizae 6-16 part.
This Chinese prescription is preferably the proportioning of 3 best effects:
Proportioning 1: Radix Codonopsis 23 parts, the Rhizoma Atractylodis Macrocephalae 22 parts, Ramulus Cinnamomi 20 parts, 15 parts, Poria, 17 parts, Semen Persicae, 14 parts, Radix Rehmanniae Preparata, the Radix Paeoniae Alba 24 parts, Ramulus Euonymi 10 parts, Radix Angelicae Sinensis 15 parts, 14 parts, Colla Corii Asini, Cortex Moutan 12 parts, Radix Salviae Miltiorrhizae 11 parts, Eupolyphaga Seu Steleophaga 5 parts, Hirudo 5 parts, Fructus Liquidambaris 13 parts, Rhizoma Cyperi 10 parts, Scorpio 4 parts, Semen Abutili 16 parts, 11 parts, Radix Glycyrrhizae.
Proportioning 2: Radix Codonopsis 25 parts, the Rhizoma Atractylodis Macrocephalae 24 parts, Ramulus Cinnamomi 22 parts, 17 parts, Poria, 15 parts, Semen Persicae, 12 parts, Radix Rehmanniae Preparata, the Radix Paeoniae Alba 22 parts, Ramulus Euonymi 8 parts, Radix Angelicae Sinensis 14 parts, 15 parts, Colla Corii Asini, Cortex Moutan 13 parts, Radix Salviae Miltiorrhizae 10 parts, Eupolyphaga Seu Steleophaga 6 parts, Hirudo 6 parts, Fructus Liquidambaris 14 parts, Rhizoma Cyperi 11 parts, Scorpio 5 parts, Semen Abutili 15 parts, 13 parts, Radix Glycyrrhizae.
Proportioning 3: Radix Codonopsis 22 parts, the Rhizoma Atractylodis Macrocephalae 21 parts, Ramulus Cinnamomi 21 parts, 14 parts, Poria, 18 parts, Semen Persicae, 16 parts, Radix Rehmanniae Preparata, the Radix Paeoniae Alba 23 parts, Ramulus Euonymi 11 parts, Radix Angelicae Sinensis 16 parts, 13 parts, Colla Corii Asini, Cortex Moutan 13 parts, Radix Salviae Miltiorrhizae 13 parts, Eupolyphaga Seu Steleophaga 4 parts, Hirudo 4 parts, Fructus Liquidambaris 16 parts, Rhizoma Cyperi 9 parts, Scorpio 4 parts, Semen Abutili 14 parts, 12 parts, Radix Glycyrrhizae.
The preparation technology this Chinese medicine being made Chinese medicine preparation is following steps:
(1) various raw medicinal material is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 8-12 times of weight, backflow decoction 3 times, each 1-3 hour, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of the 65-75% of 4-8 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 1-3 hour, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make clinical required dosage form, as dosage forms such as capsule, tablet, granule, powder, pills with the Chinese medicine preparation technology that the modern times are general.
The character of Chinese Herbs Medicines Chinese crude drug of the present invention, feature, effect and source:
Radix Codonopsis: the dry root that this product is Campanulaceae Radix Codonopsis, element spends Radix Codonopsis (RADIX CODONOPSIS PILOSULAE from Northwest of China) or radix codonpsis tangshen.Sweet in the mouth, property is put down; Return spleen, lung meridian; Invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.
The Rhizoma Atractylodis Macrocephalae: this product is the dry rhizome of the feverfew Rhizoma Atractylodis Macrocephalae.Bitter in the mouth, sweet, warm in nature; Return spleen, stomach warp; Invigorating the spleen and benefiting QI, dampness diuretic, hidroschesis, antiabortive.
Ramulus Cinnamomi: this product is the dry twig of canella Cortex Cinnamomi.Acrid in the mouth, sweet, warm in nature; GUIXIN, lung, urinary bladder channel; Diaphoresis expelling pathogenic factors from muscles, promoting the flow of QI-blood by warming the meridian, supporing yang activating QI, the flat gas that spins.
Poria: this product is the dry sclerotia of On Polyporaceae Poria.Sweet in the mouth, light, property is put down; GUIXIN, spleen, kidney channel; Eliminating dampness and diuresis, invigorating the spleen and regulating the stomach, mind tranquilizing and the heart calming.
Semen Persicae: this product is the dry mature seed of rosaceous plant Fructus Persicae or Prunus davidiana.Bitter in the mouth, sweet, property is put down; GUIXIN, liver, large intestine channel; Blood circulation promoting and blood stasis dispelling, loosening bowel to relieve constipation.
Radix Rehmanniae Preparata: this product is the Preparation process product of Radix Rehmanniae.Sweet in the mouth, slightly warm in nature; Return liver, kidney channel; Nourishing YIN and supplementing blood, beneficial essence fills out marrow.
The Radix Paeoniae Alba: this product is the dry root of ranunculaceae plant Radix Paeoniae.Bitter in the mouth, acid, cold nature; Return liver, spleen channel; Suppressing the hyperactive liver pain relieving, nourishing blood for regulating menstruation, astringing YIN to stop sweating.
Ramulus Euonymi: be branch or the aliform appurtenance of the tool fin of Celastraceae plant winged euonymus.Bitter in the mouth, cold in nature; Return Liver Channel; Removing blood stasis stimulates the menstrual flow; Removing toxic substances and promoting subsidence of swelling; Parasite killing.
Radix Angelicae Sinensis: this product is the dry root of umbelliferae angelica.Sweet in the mouth, pungent, warm in nature; Return liver, the heart, spleen channel; Enrich blood and invigorate blood circulation, menstruction regulating and pain relieving, loosening bowel to relieve constipation.
Colla Corii Asini: this product is that the skin of equine species donkey is through decocting, concentrating the solid gum made.Sweet in the mouth, property is put down; Return lung, liver, kidney channel; To enrich blood YIN nourishing, moisturize, hemostasis.
Cortex Moutan: this product is the dry root bark of ranunculaceae peony.Bitter in the mouth, pungent, cold nature; GUIXIN, liver, kidney channel; Clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling.
Radix Salviae Miltiorrhizae: this product is the dry root and rhizome of labiate Radix Salviae Miltiorrhizae.Bitter in the mouth, cold nature; GUIXIN, Liver Channel; Stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, clear away heart-fire relieving restlessness.
Eupolyphaga Seu Steleophaga: this product is the female worm dry body of Corydiidae insecticide eupolyphoge sinensis or Ji eupolyphoge sinensis.After seizure, put in boiling water and scald extremely, dry or dry.Salty in the mouth, cold in nature; Return Liver Channel; Removing blood stasis with potent drugs blood, reuniting the fractured tendons and bones.
Hirudo: this product is the dry body of Hirudinidae animal whitmania, Hirudo or Folium Salicis Babylonicae Hirudo.Salty in the mouth, hardship, property is put down; Return Liver Channel; Removing blood stasis, removing blood stasis, stimulates the menstrual flow.
Fructus Liquidambaris: this product is the drying and ripening infructescence of Hamamelidaceae Plants Chinese sweet gum.Bitter in the mouth, property is put down; Return liver, kidney channel; Dispelling, collateral-activating, diuretic stimulates the menstrual flow.
Rhizoma Cyperi: this product is the dry rhizome of sedge dried tuber.Acrid in the mouth, micro-hardship, micro-sweet, property is put down; Return liver, spleen, tri-jiao channel; Promoting QI circulation for relieving depression, menstruction regulating and pain relieving.
Scorpio: this product is the dry body of Buthidae animal Scorpio.Acrid in the mouth, property is put down; Return Liver Channel; Endogenous wind stopping spasmolytic, dispersing pathogen accumulation, removing obstruction in the collateral to relieve pain.
Semen Abutili: be the seed of Malvaceae plant Semen Abutili.Sweet in the mouth; Cold in nature; Return large intestine; Small intestinal; Liver; Lung; Stomach; Urinary bladder channel; Relieving stranguria by diuresis; Laxation relieving constipation; Stimulating milk secretion.
Radix Glycyrrhizae: this product is the dry root of glycyrrhizic legume, Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L..Sweet in the mouth, property is put down; GUIXIN, lung, spleen, stomach warp; Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.
The prescription analysis of Chinese prescription of the present invention:
Chinese prescription of the present invention is theoretical according to drug matching, and amenorrhea is being carried out to the feature in conjunction with modern pharmaceutical technique on fully dialectical basis, compatibility medical material forms again.This prescription is with Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Ramulus Cinnamomi, Semen Persicae, Semen Abutili, the Radix Paeoniae Alba for monarch drug, invigorating the spleen and replenishing QI, warming the meridian and promoting blood circulation, blood circulation promoting and blood stasis dispelling, nourishing blood for regulating menstruation, relieving stranguria by diuresis; With Ramulus Euonymi, Eupolyphaga Seu Steleophaga, Fructus Liquidambaris, Hirudo, Scorpio for ministerial drug, removing blood stasis stimulate the menstrual flow, dispelling, collateral-activating, endogenous wind stopping spasmolytic, removing obstruction in the collateral to relieve pain; With Poria, Radix Rehmanniae Preparata, Colla Corii Asini, when being classified as adjuvant drug, eliminating dampness and diuresis, nourishing YIN and supplementing blood, promoting blood flow to regulate menstruation; With Cortex Moutan, Radix Salviae Miltiorrhizae, Rhizoma Cyperi for making medicine, blood circulation promoting and blood stasis dispelling, stasis-dispelling and pain-killing, promoting QI circulation for relieving depression; With Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription, all medicines are cooperatively interacted, complements each other, synergism, thus reach the effects such as removing blood stasis stimulates the menstrual flow, nourishing blood for regulating menstruation, nourishing YIN and supplementing blood, promoting QI circulation for relieving depression, have very significant therapeutic effect to amenorrhea.
Compared with prior art, the treatment advantage such as Chinese medicine preparation of the present invention has instant effect, Be very effective, treating both the principal and secondary aspects of a disease, conditioning nourish, have no side effect, and said preparation preparation technology is simple and easy to do, efficacy stability, individual variation are little, be easy to apply.
Detailed description of the invention
Embodiment 1
Produce by the proportional quantity of Chinese prescription minimum zone of the present invention:
(1) Radix Codonopsis 16kg, Rhizoma Atractylodis Macrocephalae 15kg, Ramulus Cinnamomi 15kg, Poria 8kg, Semen Persicae 10kg, Radix Rehmanniae Preparata 7kg, Radix Paeoniae Alba 17kg, Ramulus Euonymi 6kg, Radix Angelicae Sinensis 8kg, Colla Corii Asini 9kg, Cortex Moutan 7kg, Radix Salviae Miltiorrhizae 6kg, Eupolyphaga Seu Steleophaga 3kg, Hirudo 3kg, Fructus Liquidambaris 8kg, Rhizoma Cyperi 6kg, Scorpio 2kg, Semen Abutili 11kg, Radix Glycyrrhizae 6kg is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 10 times of weight, backflow decoction 3 times, each 2 hours, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of 70% of 7 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 2 hours, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make capsule with the Chinese medicine preparation technology that the modern times are general, amount to crude drug 0.96g crude drug/capsules.
Embodiment 2
Produce by the proportional quantity of Chinese prescription maximum magnitude of the present invention:
(1) Radix Codonopsis 30kg, Rhizoma Atractylodis Macrocephalae 29kg, Ramulus Cinnamomi 25kg, Poria 22kg, Semen Persicae 24kg, Radix Rehmanniae Preparata 21kg, Radix Paeoniae Alba 31kg, Ramulus Euonymi 14kg, Radix Angelicae Sinensis 22kg, Colla Corii Asini 19kg, Cortex Moutan 17kg, Radix Salviae Miltiorrhizae 16kg, Eupolyphaga Seu Steleophaga 7kg, Hirudo 7kg, Fructus Liquidambaris 18kg, Rhizoma Cyperi 14kg, Scorpio 6kg, Semen Abutili 21kg, Radix Glycyrrhizae 16kg is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 10 times of weight, backflow decoction 3 times, each 2 hours, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of 70% of 7 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 2 hours, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make capsule with the Chinese medicine preparation technology that the modern times are general, amount to crude drug 0.96g crude drug/capsules.
Embodiment 3
Produce by proportional quantity arbitrary within the scope of Chinese prescription of the present invention:
(1) Radix Codonopsis 30kg, Rhizoma Atractylodis Macrocephalae 15kg, Ramulus Cinnamomi 25kg, Poria 8kg, Semen Persicae 24kg, Radix Rehmanniae Preparata 21kg, Radix Paeoniae Alba 17kg, Ramulus Euonymi 6kg, Radix Angelicae Sinensis 8kg, Colla Corii Asini 19kg, Cortex Moutan 17kg, Radix Salviae Miltiorrhizae 16kg, Eupolyphaga Seu Steleophaga 3kg, Hirudo 3kg, Fructus Liquidambaris 18kg, Rhizoma Cyperi 6kg, Scorpio 6kg, Semen Abutili 11kg, Radix Glycyrrhizae 16kg is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 10 times of weight, backflow decoction 3 times, each 2 hours, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of 70% of 7 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 2 hours, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make capsule with the Chinese medicine preparation technology that the modern times are general, amount to crude drug 0.96g crude drug/capsules.
Embodiment 4
Produce by the amount of Chinese prescription optimum ratio 1 of the present invention:
(1) Radix Codonopsis 23kg, Rhizoma Atractylodis Macrocephalae 22kg, Ramulus Cinnamomi 20kg, Poria 15kg, Semen Persicae 17kg, Radix Rehmanniae Preparata 14kg, Radix Paeoniae Alba 24kg, Ramulus Euonymi 10kg, Radix Angelicae Sinensis 15kg, Colla Corii Asini 14kg, Cortex Moutan 12kg, Radix Salviae Miltiorrhizae 11kg, Eupolyphaga Seu Steleophaga 5kg, Hirudo 5kg, Fructus Liquidambaris 13kg, Rhizoma Cyperi 10kg, Scorpio 4kg, Semen Abutili 16kg, Radix Glycyrrhizae 11kg is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 10 times of weight, backflow decoction 3 times, each 2 hours, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of 70% of 7 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 2 hours, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make capsule with the Chinese medicine preparation technology that the modern times are general, amount to crude drug 0.96g crude drug/capsules.
Embodiment 5
Produce by the amount of Chinese prescription optimum ratio 2 of the present invention:
(1) Radix Codonopsis 25kg, Rhizoma Atractylodis Macrocephalae 24kg, Ramulus Cinnamomi 22kg, Poria 17kg, Semen Persicae 15kg, Radix Rehmanniae Preparata 12kg, Radix Paeoniae Alba 22kg, Ramulus Euonymi 8kg, Radix Angelicae Sinensis 14kg, Colla Corii Asini 15kg, Cortex Moutan 13kg, Radix Salviae Miltiorrhizae 10kg, Eupolyphaga Seu Steleophaga 6kg, Hirudo 6kg, Fructus Liquidambaris 14kg, Rhizoma Cyperi 11kg, Scorpio 5kg, Semen Abutili 15kg, Radix Glycyrrhizae 13kg is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 10 times of weight, backflow decoction 3 times, each 2 hours, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of 70% of 7 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 2 hours, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make capsule with the Chinese medicine preparation technology that the modern times are general, amount to crude drug 0.96g crude drug/capsules.
Embodiment 6
Produce by the amount of Chinese prescription optimum ratio 3 of the present invention:
(1) Radix Codonopsis 22kg, Rhizoma Atractylodis Macrocephalae 21kg, Ramulus Cinnamomi 21kg, Poria 14kg, Semen Persicae 18kg, Radix Rehmanniae Preparata 16kg, Radix Paeoniae Alba 23kg, Ramulus Euonymi 11kg, Radix Angelicae Sinensis 16kg, Colla Corii Asini 13kg, Cortex Moutan 13kg, Radix Salviae Miltiorrhizae 13kg, Eupolyphaga Seu Steleophaga 4kg, Hirudo 4kg, Fructus Liquidambaris 16kg, Rhizoma Cyperi 9kg, Scorpio 4kg, Semen Abutili 14kg, Radix Glycyrrhizae 12kg is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 10 times of weight, backflow decoction 3 times, each 2 hours, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of 70% of 7 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 2 hours, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make capsule with the Chinese medicine preparation technology that the modern times are general, amount to crude drug 0.96g crude drug/capsules.
Embodiment 7
Animal toxicity test
1, test drug: Chinese medicine capsules obtained by embodiment of the present invention 1-6, abolishes capsule shells, adds purified water and is modulated into magma.
Test animal used: regular grade NIH mice, body weight 23g ± 4g, male and female half and half, female mice is all without pregnant; SD rat, original body mass 215 ± 11g, male and female half and half, female rats is all without pregnant.
2, acute toxicity test
Application mice carries out acute toxicity testing and shows: mouse stomach Chinese medicine oral liquid of the present invention, under 711.4g crude drug/kg dosage, after administration, mice occurs that light activity reduces, 1.5 hours recover normal, Continuous Observation 7 days after administration, none dead mouse, and overall health of patients, diet, take the photograph water, urine and body weight increase all normal.Experimental result shows: the maximum dosage-feeding of mouse stomach Chinese medicine capsules of the present invention is 711.4g crude drug/kg/d(LD
50> 711.4g crude drug/kg).Chinese medicine preparation clinical application every day total amount of the present invention is 0.16g crude drug/kg/d; By weighing machine, the dosis tolerata of mouse stomach Chinese medicine preparation of the present invention is 4446.3 times of clinical patient.Point out this medicine acute toxicity extremely low, clinical drug safety.
3, long term toxicity test
Select SD rat, give the Chinese medicine capsules of the present invention of Three doses: 28.8g crude drug/kg, 9.6g crude drug/kg, 3.2g crude drug/kg(per weight dosage is by 20 times of calculating of clinical application amount, and clinical application amount is according to Pharmacopoeia of the People's Republic of China version in 2010).Every day gavage once, continuous 90 days, after last administration 24 hours respectively group live and kill 1/2 animal (male and female half and half), all the other 1/2 animals continue that observations are alive after 2 weeks kills.Duration of test observes outward appearance, general behavior, food ration, the body weight change of animal; Within after administration 90 days, within 2 weeks, carry out the index checking such as hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crea, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology with drug withdrawal.
Result of the test shows: Chinese medicine preparation of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception; Three dosage groups and matched group hematological examination, blood biochemical analysis, urine biochemical analysis all in normal range, without significant difference between group; Each group of main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.This test dosage is respectively 180,60,20 times of clinical medicine dose, according to result of the test: Chinese medicine preparation of the present invention has no significant effect rat in high, medium and low three dosage (28.8g crude drug/kg, 9.6g crude drug/kg, 3.2g crude drug/kg) administration in continuous 90 days, without clear and definite toxicity target organ and sensitive indicator, convalescent period observation also has no retardance toxic reaction, points out the dose safety of Chinese medicine preparation clinical practice of the present invention very high.
Embodiment 8
The pharmacological testing of the made capsule of embodiment of the present invention 4-6
The impact of animal ovary function is tested
1, experimental animal: New Zealand germline white doe, 3-4 monthly age, body weight 1.9-2.6kg.
2, trial drug:
Test 1 group: the made capsule of the embodiment of the present invention 4, abolish capsule shells, adding distil water is modulated into scattered paste shape, for subsequent use;
Test 2 groups: the made capsule of the embodiment of the present invention 5, abolish capsule shells, adding distil water is modulated into scattered paste shape, for subsequent use;
Test 3 groups: the made capsule of the embodiment of the present invention 6, abolish capsule shells, adding distil water is modulated into scattered paste shape, for subsequent use;
3, test method: animal subject raises 2 weeks in advance, treats body temperature relative constancy, after there is oestrous cycle sample change in external genital, and random packet.Wherein one group is matched group, and all the other 3 groups are test group.Aminoglutethimidium (call ammonia in the following text to lead, it is for subsequent use that distilled water is made into 10mg/ml suspension) 6mg/kg adding distil water; Timing in morning every day is inserted stomach tube throwing ammonia and is led; Fill with after 5h to test medicine, matched group gives equivalent distilled water, the administration of trial drug group coloclysis, and dosage is 160mg/kg, continuous 15 days.To external genital, the change of vagina epithelium exfoliative cyte oestrous cycle of animal subject, by only Continuous Observation analysis; Timing in afternoon every day vaginal smear, methylene blue, cytolgical examination.Test the 16th, ear vein is taken a blood sample, separated plasma at once, presses with radioimmunology the content that medicine box operating instruction measures β-EP; After blood sampling, air tap inserting method puts to death animal, wins body of uterus (comprising fallopian tube and ovary), rejects fat and its hetero-organization, after filter paper is slightly wiped, puts into balance respectively and accurately to weigh the weight in wet base of ovary; Under ovary is placed in anatomical lens; After measuring follicle size and counting, with body of uterus with fixing.Embedding, section, HE dyeing, carry out histological examination.
4, statistical procedures
Measurement data represents with mean ± standard deviation, adopts SPSS10.0 software kit to carry out statistical analysis, and the analysis of many Sets of Measurement Datas adopts One way ANOVA, and multiple comparisons adopts LSD, represents that difference has significant with P < 0.05.
5, result of the test
Compared by vaginal exfoliated cell smear, Plasma Beta-EP level, follicle number, ovary compare list and are expressed as follows:
Each test group vaginal exfoliated cell smear comparative statistics analytical table of table 1 (
± SD)
Note: * P < 0.01 compared with matched group.
The horizontal comparative statistics analytical table of each test group Plasma Beta of table 2-EP (
± SD)
Note: * P < 0.01 compared with matched group.
Each test group follicle number of table 3, ovary comparative statistics analytical table (
± SD)
Note: * P < 0.01 compared with matched group.
6, conclusion (of pressure testing)
Above-mentioned test shows, alone ammonia leads group rabbit, performance ovarian hypofunction: ovary is without mature follicle, and endometrium is thin.Test the 1 group-rabbit of testing 3 groups then to extend rutting period, the mature follicle of ovary increases, uterus
The obvious hypertrophy of inner membrance, thickening, the performance that the ovarian functions such as body of gland increased number are vigorous.Show the toxicity that Chinese medicine energy antagonism ammonia of the present invention is led, make β-EP content recover normal, prompting β-EP may participate in the functional activity of gonad axis, especially has promotion, regulating action to ovarian function.In above-mentioned test, test 2 groups and show particularly outstanding in three test group, illustrate that the curative effect of the made Chinese medicine preparation of the formula proportion of embodiment 5 is the most remarkable.
Embodiment 9 clinical effectiveness is tested
1, testing data
Certain gynecological of First People's Hospital of city provides 131 experimenters, age 19-34 year, 29 years old mean age for this test.Wherein, 49 examples are given birth to, and 33 examples have abortion history, and 23 examples have oral contraceptive history, and 26 examples get birth control apparatus history.Physiologic amenorrhea, preadolescence, trimester of pregnancy, age of sucking and postmenopause except all cases is equal.All patients all agree to participate in this test.
2, test method
All patients give the embodiment of the present invention 5 made capsule, oral capsule, every day 2 times, each 5, amount to crude drug amount and are about 9.6g crude drug/sky.Within 4 weeks, be a course for the treatment of, treat 4 courses for the treatment of continuously.
3, criterion
Cure: after treatment, menstruation recovery is normal, various hormonal readiness recovers, and makes a definite diagnosis return to one's perfect health through doctor.
Effective: after treatment, menstruation is risen, but irregular, and hormonal readiness makes moderate progress.
Invalid: without any improvement, even more serious after treatment.
4, therapeutic outcome
131 experimenters have 128 to adhere to whole test, and 3 examples come off; Wherein recovery from illness 62 example, effective 59 examples, invalid 7 examples.Cure rate is 48.4%, and total effective rate is 94.5%.
5, conclusion
Clinical test results shows, and Chinese medicine preparation of the present invention has very significant therapeutic effect in treatment amenorrhea, and therefore, Chinese medicine preparation of the present invention has significant technical advantage, achieves beat all technological achievement.
Claims (6)
1. treat a Chinese medicine preparation for amenorrhea, it is characterized in that being formed by the Raw material processing of following weight portion: Radix Codonopsis 16-30 part, Rhizoma Atractylodis Macrocephalae 15-29 part, Ramulus Cinnamomi 15-25 part, Poria 8-22 part, Semen Persicae 10-24 part, Radix Rehmanniae Preparata 7-21 part, Radix Paeoniae Alba 17-31 part, Ramulus Euonymi 6-14 part, Radix Angelicae Sinensis 8-22 part, Colla Corii Asini 9-19 part, Cortex Moutan 7-17 part, Radix Salviae Miltiorrhizae 6-16 part, Eupolyphaga Seu Steleophaga 3-7 part, Hirudo 3-7 part, Fructus Liquidambaris 8-18 part, Rhizoma Cyperi 6-14 part, Scorpio 2-6 part, Semen Abutili 11-21 part, Radix Glycyrrhizae 6-16 part.
2. the Chinese medicine preparation for the treatment of amenorrhea according to claim 1, is characterized in that being formed by the Raw material processing of following weight portion: Radix Codonopsis 23 parts, the Rhizoma Atractylodis Macrocephalae 22 parts, Ramulus Cinnamomi 20 parts, 15 parts, Poria, 17 parts, Semen Persicae, 14 parts, Radix Rehmanniae Preparata, the Radix Paeoniae Alba 24 parts, Ramulus Euonymi 10 parts, Radix Angelicae Sinensis 15 parts, 14 parts, Colla Corii Asini, Cortex Moutan 12 parts, Radix Salviae Miltiorrhizae 11 parts, Eupolyphaga Seu Steleophaga 5 parts, Hirudo 5 parts, Fructus Liquidambaris 13 parts, Rhizoma Cyperi 10 parts, Scorpio 4 parts, Semen Abutili 16 parts, 11 parts, Radix Glycyrrhizae.
3. the Chinese medicine preparation for the treatment of amenorrhea according to claim 1, is characterized in that being formed by the Raw material processing of following weight portion: Radix Codonopsis 25 parts, the Rhizoma Atractylodis Macrocephalae 24 parts, Ramulus Cinnamomi 22 parts, 17 parts, Poria, 15 parts, Semen Persicae, 12 parts, Radix Rehmanniae Preparata, the Radix Paeoniae Alba 22 parts, Ramulus Euonymi 8 parts, Radix Angelicae Sinensis 14 parts, 15 parts, Colla Corii Asini, Cortex Moutan 13 parts, Radix Salviae Miltiorrhizae 10 parts, Eupolyphaga Seu Steleophaga 6 parts, Hirudo 6 parts, Fructus Liquidambaris 14 parts, Rhizoma Cyperi 11 parts, Scorpio 5 parts, Semen Abutili 15 parts, 13 parts, Radix Glycyrrhizae.
4. the Chinese medicine preparation for the treatment of amenorrhea according to claim 1, is characterized in that being formed by the Raw material processing of following weight portion: Radix Codonopsis 22 parts, the Rhizoma Atractylodis Macrocephalae 21 parts, Ramulus Cinnamomi 21 parts, 14 parts, Poria, 18 parts, Semen Persicae, 16 parts, Radix Rehmanniae Preparata, the Radix Paeoniae Alba 23 parts, Ramulus Euonymi 11 parts, Radix Angelicae Sinensis 16 parts, 13 parts, Colla Corii Asini, Cortex Moutan 13 parts, Radix Salviae Miltiorrhizae 13 parts, Eupolyphaga Seu Steleophaga 4 parts, Hirudo 4 parts, Fructus Liquidambaris 16 parts, Rhizoma Cyperi 9 parts, Scorpio 4 parts, Semen Abutili 14 parts, 12 parts, Radix Glycyrrhizae.
5. a preparation method for the Chinese medicine preparation of the treatment amenorrhea as described in as arbitrary in claim 1-4, is characterized in that comprising following processing step:
(1) various raw medicinal material is accurately taken in prescription ratio;
(2) Ramulus Cinnamomi, Poria, Semen Persicae, the Radix Paeoniae Alba, Ramulus Euonymi, Cortex Moutan, Fructus Liquidambaris, Rhizoma Cyperi, Semen Abutili are pulverized respectively, be mixed and add the purified water of 8-12 times of weight, backflow decoction 3 times, each 1-3 hour, filter (reservation filtering residue), merging filtrate, when being condensed into 60 DEG C, relative density is the water extracting liquid of 1.15-1.20, for subsequent use;
(3) Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Eupolyphaga Seu Steleophaga, Hirudo, Scorpio are pulverized respectively, add the ethanol of the 65-75% of 4-8 times of weight together with step (2) filtering residue, reflux, extract, 3 times, each 1-3 hour, merge alcohol extract, distilling under reduced pressure removing ethanol, obtains alcohol extraction concentrated solution;
(4) by Colla Corii Asini molten, mix while hot with water extracting liquid and alcohol extraction concentrated solution, relative density 1.25-1.30 when being concentrated into 60 DEG C, drying under reduced pressure becomes dry extract, pulverizes, and grinding is crossed 120 mesh sieves, obtained fine powder, for subsequent use;
(5) Radix Glycyrrhizae is cut into slices, fry to deep yellow, pulverize, grinding, cross 120 mesh sieves, obtain fine powder, for subsequent use;
(6) by step (4) and the mixing of step (5) gained fine powder, add suitable adjuvant, make clinical required dosage form with the Chinese medicine preparation technology that the modern times are general.
6., according to the arbitrary described a kind of Chinese medicine preparation for the treatment of amenorrhea of claim 1-4, it is characterized in that, described Chinese medicine preparation can make capsule, tablet, granule, powder and pill.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104491594A (en) * | 2014-12-05 | 2015-04-08 | 李秀玲 | Pharmaceutical composition for treating premature ovarian failure |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101926880A (en) * | 2010-08-30 | 2010-12-29 | 黄远吉 | Chinese medicinal composition for treating amenorrhea and endometritis of women |
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| CN101926880A (en) * | 2010-08-30 | 2010-12-29 | 黄远吉 | Chinese medicinal composition for treating amenorrhea and endometritis of women |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104491594A (en) * | 2014-12-05 | 2015-04-08 | 李秀玲 | Pharmaceutical composition for treating premature ovarian failure |
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Application publication date: 20150408 |