CN104547712A - Traditional Chinese medicine preparation for treating climacteric syndrome and preparation method of traditional Chinese medicine preparation - Google Patents

Traditional Chinese medicine preparation for treating climacteric syndrome and preparation method of traditional Chinese medicine preparation Download PDF

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CN104547712A
CN104547712A CN201510034669.7A CN201510034669A CN104547712A CN 104547712 A CN104547712 A CN 104547712A CN 201510034669 A CN201510034669 A CN 201510034669A CN 104547712 A CN104547712 A CN 104547712A
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parts
radix
chinese medicine
medicine preparation
rhizoma
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田洪荣
杨斐
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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Abstract

The invention discloses a traditional Chinese medicine preparation for treating climacteric syndrome, relating to the technical field of traditional Chinese medicines. The traditional Chinese medicine preparation is prepared from the following raw materials: fructus ligustri lucidi, radix paeoniae alba, rhizoma dioscoreae, herba epimedii, rhizoma curculigins, semen cuscutae, caulis polygoni multiflori, fructus rubi, herba leonuri, radix lithospermi, radix rehmanniae recens, radix codonopsis, radix astragali seu hedysari, ramulus uncariae cum uncis, Wheat from Huang-huai Area, semen platycladi, rhizoma polygonati odorati, radix stephaniae tetrandrae and radix glycyrrhizae. The traditional Chinese medicine preparation has the therapeutic advantages of good treatment effect, fast effect taking, treatment of the symptoms and the causes, small therapeutic difference of individuals and the like, and the preparation technology also has the technical advantages of simple and convenient operation, stable medical effect, easy popularization and the like.

Description

a kind of Chinese medicine preparation for the treatment of climacteric syndrome and preparation method thereof
Technical field
The present invention relates to a kind of Chinese medicine preparation for the treatment of climacteric syndrome, belong to technical field of Chinese medicines.
Technical background
Perimenopausal syndrome, also known as climacteric syndrome (MPS), refers to occur before and after postmenopausal women gonadal hormone fluctuation or a series of based on function disorder of autonomic nervous system caused by reducing, with one group of syndrome of neuropsychological symptom.Menopause can be divided into natural menopause and artificial menopause two kinds.Natural menopause refers to that in ovary, follicle is used up, or remaining follicle loses reaction to promoting sexual gland hormone, and follicle is no longer grown and secreted estrogen, can not stimulate endometrial growth, cause menopause.Artificial menopause refers to excision bilateral ovaries or stops ovarian function with additive method, as radiotherapy and chemotherapy etc.At present, the multiplex Hormone Replacement Therapy of doctor trained in Western medicine, this therapy for treating weak effect, side effect are large.
The traditional Chinese medical science thinks that climacteric syndrome is caused by suffering from a deficiency of the kidney.Annual income climacteric, function degradation, deficiency of kidney-QI, rushes conception vessel void, and menses will exhaust, asthenia of essence and blood,, there is deficiency of kidney yin, sun lose hiding in imbalance between YIN and YANG, or kidney yang deficiency, passages through which vital energy circulates lose and partially win partially negative phenomenon in kidney yin kidney yangs such as replenishing vital QI with drugs of warm nature, thus cause function not normal, therefore suffer from a deficiency of the kidney be pathogenic this.Due to the difference of physical factors, the clinical different manifestations having again deficiency in both YIN and YANG in insufficiency of kidney-YANG, deficiency of the kidney yin or kidney, and the most common with deficiency of the kidney yin.But according to internal organs correlation theory and clinical practice situation, people recognize that alone suffering from a deficiency of the kidney explains that this disease is far from being enough, it also and QI and blood dirty with the heart, liver, spleen three rush appoint relevant.The liver and the kidney have a common source, deficiency of kidney-YIN, the kidney failing to nourish the liver, causes deficiecny of liver-YIN, excessive rising of liver-YANG; Deficiency of kidney-YIN, discordance between water(the kidney)and fire(the heart), disarmony between the heart and kidney then visible heart-fire is solely high, wasting QI-blood; Insufficiency of kidney-YANG, decline of the fire from the gate of life, spleen sun loses warm, can cause hypoactivity of spleen-YANG, deficiency of spleen-YANG and kidneyYANG; Kidney yang virtual loss, unit god loses and supports, then can cause manner and dissipate.Meanwhile, asthenia of essence and blood, punching appoint obstructed be morbidity essential condition.In recent years, along with the further investigation to " blood stasis due to renal deficiency " pathogenesis, find blood stasis as Secondary cases pathological product this in kidney so that disorder of QI and blood, venation astringent or styptic treatment for spontaneous sweating, stagnant and smooth, function is not normal.Concrete differentiation of symptoms and signs for classification of syndrome with treat as follows:
1, kidney-yin deficiency type
Primary symptom: dizziness and tinnitus, soreness of the waist and knees, hectic fever, sweating, dysphoria with feverish sensation in the chest palms and soles, the flushing flushing in zygomatic region, dry mouth and constipation, oliguria with reddish urine, premenstrua or front and back are irregularly, scarlet through color, and amount more or less.The few tongue of red tongue, thready and rapid pulse.The rule for the treatment of: nourish kidney yin, YANG hyperactivity suppressing of slightly holding concurrently.
2, kidney-yang deficiency type
Primary symptom: lethargy, coldness of the body with chills, the acid of waist knee joint is cold, indigestion and loss of appetite abdominal distention, and large loose stool is thin, or light through row amount polychrome, puffiness of the face and limbs, nocturia or urinary incontinence.Light red tongue or corpulent tongue is tender, there is indentation on limit, white and thin fur, deep,thready and forceless pulse.The rule for the treatment of: warming and recuperating the kidney-YANG.
3, kidney yin and yang deficiency type
Primary symptom: soreness of waist and knee, dizzy vexed, or feel depressed, fatigue and weak, fear of cold is afraid of cold, time and warm sweating.Thin white fur of tongue, thready pulse.
The rule for the treatment of: nourishing kidney invigorating YANG, replenishing CHONG and REN meridians by supplementing the essence and blood.
The present invention, on the basis of above-mentioned dialectical cognition, takes into account each typing, forms core of the present invention research and development intention.
Summary of the invention
The technical problem to be solved in the present invention has two, and one is that a kind of Chinese prescription of invention makes it have good therapeutic effect to climacteric syndrome; Another invents a manufacturing process, Chinese prescription is developed to the Chinese medicine finished product preparation that modern clinic needs.
In order to solve this two technical problems, inventor's foundation Traditional Chinese medical theory is to the dialectical understanding of climacteric syndrome, in conjunction with self tcm clinical practice experience for many years and the ill feature of modern, take into full account the change of modern pharmaceutical technique to the drug effect property of medicine, again compatibility medical material, from the increase and decrease of clinical practice science, the Chinese prescription that final invention one is brand-new, and develop corresponding preparation technology and be made into finished product preparation, to climacteric syndrome, there is therapeutic effect very significantly.This Chinese prescription is (with parts by weight):
Fructus Ligustri Lucidi 8-16 part, Radix Paeoniae Alba 14-26 part, Rhizoma Dioscoreae 10-20 part, Herba Epimedii 9-19 part, Rhizoma Curculiginis 8-18 part, Semen Cuscutae 10-22 part, Caulis Polygoni Multiflori 7-17 part, Fructus Rubi 9-21 part, Herba Leonuri 11-23 part, Radix Arnebiae (Radix Lithospermi) 7-17 part, Radix Rehmanniae 10-22 part, Radix Codonopsis 8-18 part, Radix Astragali 10-21 part, Ramulus Uncariae Cum Uncis 11-22 part, Huai wheat 19-31 part, Semen Platycladi 6-14 part, Rhizoma Polygonati Odorati 6-12 part, Radix Stephaniae Tetrandrae 5-11 part, Radix Glycyrrhizae 9-14 part.
In practice process, 4 that the sum up prescription proportionings with best curative effect are respectively:
Proportioning 1: Fructus Ligustri Lucidi 12 parts, the Radix Paeoniae Alba 20 parts, Rhizoma Dioscoreae 15 parts, Herba Epimedii 14 parts, Rhizoma Curculiginis 13 parts, Semen Cuscutae 16 parts, Caulis Polygoni Multiflori 12 parts, Fructus Rubi 15 parts, Herba Leonuri 17 parts, Radix Arnebiae (Radix Lithospermi) 12 parts, 16 parts, the Radix Rehmanniae, Radix Codonopsis 13 parts, the Radix Astragali 15 parts, Ramulus Uncariae Cum Uncis 16 parts, Huai wheat 25 parts, Semen Platycladi 10 parts, Rhizoma Polygonati Odorati 9 parts, Radix Stephaniae Tetrandrae 8 parts, 11 parts, Radix Glycyrrhizae.
Proportioning 2: Fructus Ligustri Lucidi 14 parts, the Radix Paeoniae Alba 21 parts, Rhizoma Dioscoreae 16 parts, Herba Epimedii 15 parts, Rhizoma Curculiginis 12 parts, Semen Cuscutae 15 parts, Caulis Polygoni Multiflori 13 parts, Fructus Rubi 17 parts, Herba Leonuri 16 parts, Radix Arnebiae (Radix Lithospermi) 13 parts, 15 parts, the Radix Rehmanniae, Radix Codonopsis 14 parts, the Radix Astragali 17 parts, Ramulus Uncariae Cum Uncis 14 parts, Huai wheat 27 parts, Semen Platycladi 11 parts, Rhizoma Polygonati Odorati 10 parts, Radix Stephaniae Tetrandrae 9 parts, 12 parts, Radix Glycyrrhizae.
Proportioning 3: Fructus Ligustri Lucidi 13 parts, the Radix Paeoniae Alba 22 parts, Rhizoma Dioscoreae 14 parts, Herba Epimedii 17 parts, Rhizoma Curculiginis 15 parts, Semen Cuscutae 14 parts, Caulis Polygoni Multiflori 15 parts, Fructus Rubi 16 parts, Herba Leonuri 15 parts, Radix Arnebiae (Radix Lithospermi) 13 parts, 14 parts, the Radix Rehmanniae, Radix Codonopsis 12 parts, the Radix Astragali 14 parts, Ramulus Uncariae Cum Uncis 15 parts, Huai wheat 24 parts, Semen Platycladi 12 parts, Rhizoma Polygonati Odorati 8 parts, Radix Stephaniae Tetrandrae 9 parts, 12 parts, Radix Glycyrrhizae.
Proportioning 4: Fructus Ligustri Lucidi 14 parts, the Radix Paeoniae Alba 23 parts, Rhizoma Dioscoreae 16 parts, Herba Epimedii 16 parts, Rhizoma Curculiginis 14 parts, Semen Cuscutae 15 parts, Caulis Polygoni Multiflori 14 parts, Fructus Rubi 17 parts, Herba Leonuri 18 parts, Radix Arnebiae (Radix Lithospermi) 13 parts, 15 parts, the Radix Rehmanniae, Radix Codonopsis 14 parts, the Radix Astragali 16 parts, Ramulus Uncariae Cum Uncis 17 parts, Huai wheat 26 parts, Semen Platycladi 11 parts, Rhizoma Polygonati Odorati 11 parts, Radix Stephaniae Tetrandrae 9 parts, 11 parts, Radix Glycyrrhizae.
The preparation technology of technical solution problem of the present invention is following steps:
(1) take ingredients by Chinese prescription, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 6-10 times weight, soak 10-24 hour, backflow decocts 2-4 hour, filter, then add the purified water of initial medical material 3-7 times weight, backflow decocts 1-3 hour, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 65-80% of 4-7 times of weight, reflux, extract, 2 times, each 1-4 hour, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make the finished dosage forms of current clinical needs, as dosage forms such as tablet, pill, granule, capsule, powder, oral liquid, syrups.
Source, the nature and flavor of the present invention's Chinese crude drug used, return through and effect:
Fructus Ligustri Lucidi: this product is the dry mature fruit of Oleaceae plants Fructus Ligustri Lucidi.Sweet in the mouth, hardship, cool in nature; Return liver, kidney channel; Nourishing the liver and kidney, improving eyesight black hair.
The Radix Paeoniae Alba: this product is the dry root of ranunculaceae plant Radix Paeoniae.Bitter in the mouth, acid, cold nature; Return liver, spleen channel; Suppressing the hyperactive liver pain relieving, nourishing blood for regulating menstruation, astringing YIN to stop sweating.
Rhizoma Dioscoreae: this product is the dry rhizome of Dioscoreaceae plant Rhizoma Dioscoreae.Sweet in the mouth, property is put down; Return spleen, lung, kidney channel; Spleen reinforcing nourishing the stomach, promote the production of body fluid lung benefiting, the kidney invigorating arresting seminal emission.
Herba Epimedii: this product is the dry aerial parts of Berberidaceae plant Herba Epimedii, Epimedium sagittatum, E. Pubescens, Epimedium wushanense or Herba Epimedii.Acrid in the mouth, sweet, warm in nature; Return liver, kidney channel; Kidney-replenishing, bone and muscle strengthening, wind-damp dispelling.
Rhizoma Curculiginis: this product is the dry rhizome of amrallid Rhizoma Curculiginis.Acrid in the mouth, hot in nature; Return kidney,liver,spleen warp; Kidney-replenishing, bone and muscle strengthening, dispelling cold and dampness.
Semen Cuscutae: this product is the dry mature seed of convolvulus cuscuta plant.Sweet in the mouth, warm in nature; Return liver,kidney,spleen warp; Nourishing the liver and kidney, controlling nocturnal emission with astringent drugs reducing urination, antiabortive, improving eyesight, antidiarrheal.
Caulis Polygoni Multiflori: be rattan or the leaf bine stem of polygonum multiflorum thunb.Sweet and slightly bitter taste, property is put down; Enter the heart, Liver Channel; Nourish heart, calm the nerves, dredging collateral, dispel the wind.
Fructus Rubi: this product is the dry fruit of Rosaceae rubus rubus chingii Hu.Sweet in the mouth, acid, warm in nature; Return kidney, urinary bladder channel; Kidney tonifying, controlling nocturnal emission with astringent drugs, reducing urination.
Herba Leonuri: this product is the fresh of labiate Herba Leonuri or dry aerial parts.Bitter in the mouth, pungent, cold nature; Return liver, pericardium channel; Promoting blood flow to regulate menstruation, inducing diuresis to remove edema.
Radix Arnebiae (Radix Lithospermi): this product is the dry root of comfrey lithospermum euchromum Royle, Radix Arnebiae (Radix Lithospermi) or arnebia guttata Bunge.Sweet in the mouth, salty, cold in nature; GUIXIN, Liver Channel; Removing heat from blood, invigorates blood circulation, removing toxic substances rash.
The Radix Rehmanniae: this product is the fresh of scrophulariaceae rehmannia glutinosa plant or dried root.Sweet in the mouth, cold in nature; GUIXIN, liver, kidney channel; Clearing away heat and cooling blood, yin nourishing, promotes the production of body fluid.
Radix Codonopsis: the dry root that this product is Campanulaceae Radix Codonopsis, element spends Radix Codonopsis (RADIX CODONOPSIS PILOSULAE from Northwest of China) or radix codonpsis tangshen.Sweet in the mouth, property is put down; Return spleen, lung meridian; Invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.
The Radix Astragali: this product is the dry root of leguminous plant Radix Astagali or Radix Astragali.Sweet in the mouth, warm in nature; Return lung, spleen channel; Invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, expelling pus and promoting granulation.
Ramulus Uncariae Cum Uncis: this product is the dry stem and branch with belt hook of Maguireothamnus speciosus Ramulus Uncariae Cum Uncis, Ramulus Uncariae macrophyllae, Ramulus Uncariae Cum Uncis, Uncaria sinensis (Oliv.) Havil. or stockless fruit Ramulus Uncariae Cum Uncis.Sweet in the mouth, cool in nature; Return liver, pericardium channel; Heat clearing away suppressing the hyperactive liver, dispelling wind and relieving convulsion.
Huai wheat: this product is the fruit of grass Semen Tritici aestivi.Sweet in the mouth, property is put down; Enter heart channel; Tranquilizing by nourishing the heart.
Semen Platycladi: this product is the dry mature kernal of Cupressaceae plant Cacumen Platycladi.Sweet in the mouth, property is put down; GUIXIN, kidney, large intestine channel; Tranquilizing by nourishing the heart, hidroschesis, intestine moistening.
Rhizoma Polygonati Odorati: this product is the dry rhizome of liliaceous plant Rhizoma Polygonati Odorati.Sweet in the mouth, cold nature; Attach to the lung and stomach meridians; Nourishing YIN to relieve dryness, promoting the production of body fluid to quench thirst.
Radix Stephaniae Tetrandrae: this product is the dry root of menispermaceous plants Radix stephaniae tetrandrae.Bitter in the mouth, cold in nature; Return bladder, lung meridian; Inducing diuresis to remove edema, wind-expelling pain-stopping.
Radix Glycyrrhizae: this product is the dry root of glycyrrhizic legume, Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L..Sweet in the mouth, property is put down; GUIXIN, lung, spleen, stomach warp; Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.
The side of Chinese prescription of the present invention separates:
Chinese prescription of the present invention is with the Radix Paeoniae Alba, Huai wheat, Herba Leonuri, Semen Cuscutae, the Radix Rehmanniae for monarch drug, astringing YIN to stop sweating, tranquilizing by nourishing the heart, promoting blood flow to regulate menstruation, nourishing liver and kidney, clearing away heat and cooling blood; With Rhizoma Dioscoreae, Fructus Rubi, the Radix Astragali, Ramulus Uncariae Cum Uncis, Herba Epimedii for ministerial drug, the lung benefiting that promotes the production of body fluid, supplementing the kidney to control the nocturnal, invigorating QI to consolidate the body surface resistance, dispelling wind and relieving convulsion, the kidney invigorating bone strengthening; With Fructus Ligustri Lucidi, Rhizoma Curculiginis, Caulis Polygoni Multiflori, Semen Platycladi for adjuvant drug, improving eyesight black hair, YANG invigorating damp eliminating, hidroschesis of calming the nerves; With Radix Arnebiae (Radix Lithospermi), Radix Codonopsis, Rhizoma Polygonati Odorati, Radix Stephaniae Tetrandrae for making medicine, removing toxic substances rash, invigorating the spleen and replenishing QI, nourishing YIN to relieve dryness, wind-expelling pain-stopping; With Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription, enable all medicine synergism, complement each other, thus reach the effects such as astringing YIN to stop sweating, tranquilizing by nourishing the heart, nourishing liver and kidney, YANG invigorating damp eliminating, nourishing YIN to relieve dryness, to climacteric syndrome, there is very significant therapeutic effect.
Compared with prior art, Chinese medicine preparation of the present invention has the treatment advantages such as therapeutic effect is good, instant effect, treating both the principal and secondary aspects of a disease, curative effect individual variation are little, and its preparation technology also has easy and simple to handle, efficacy stability, is easy to technical advantages such as applying.
Detailed description of the invention
In order to verify preparation technology of the present invention and prescription, and provide test drug for follow-up animal toxicity test, clinical trial, embodiment 1-7 is respectively the preparation by different ratio actual production, and embodiment 8 is animal toxicity test, and embodiment 9 is clinical trial.
Embodiment 1
Preparation is produced in the minima ratio of Chinese prescription scope of the present invention:
(1) take by Chinese prescription: Fructus Ligustri Lucidi 800g, Radix Paeoniae Alba 1400g, Rhizoma Dioscoreae 1000g, Herba Epimedii 900g, Rhizoma Curculiginis 800g, Semen Cuscutae 1000g, Caulis Polygoni Multiflori 700g, Fructus Rubi 900g, Herba Leonuri 1100g, Radix Arnebiae (Radix Lithospermi) 700g, Radix Rehmanniae 1000g, Radix Codonopsis 800g, Radix Astragali 1000g, Ramulus Uncariae Cum Uncis 100g, Huai wheat 1900g, Semen Platycladi 600g, Rhizoma Polygonati Odorati 600g, Radix Stephaniae Tetrandrae 500g, Radix Glycyrrhizae 900g, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 8 times of weight, soak 16 hours, backflow decoction 3 hours, filter, then add the purified water of initial medical material 5 times of weight, backflow decoction 2 hours, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 75% of 6 times of weight, reflux, extract, 2 times, each 2 hours, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make granule, specification is: amount to 0.36g crude drug/g granule.
Embodiment 2
Preparation is produced in the maximum ratio of Chinese prescription scope of the present invention:
(1) take by Chinese prescription: Fructus Ligustri Lucidi 1600g, Radix Paeoniae Alba 2600g, Rhizoma Dioscoreae 2000g, Herba Epimedii 1900g, Rhizoma Curculiginis 1800g, Semen Cuscutae 2200g, Caulis Polygoni Multiflori 1700g, Fructus Rubi 2100g, Herba Leonuri 2300g, Radix Arnebiae (Radix Lithospermi) 1700g, Radix Rehmanniae 2200g, Radix Codonopsis 1800g, Radix Astragali 2100g, Ramulus Uncariae Cum Uncis 2200g, Huai wheat 3100g, Semen Platycladi 1400g, Rhizoma Polygonati Odorati 1200g, Radix Stephaniae Tetrandrae 1100g, Radix Glycyrrhizae 1400g, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 8 times of weight, soak 16 hours, backflow decoction 3 hours, filter, then add the purified water of initial medical material 5 times of weight, backflow decoction 2 hours, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 75% of 6 times of weight, reflux, extract, 2 times, each 2 hours, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make granule, specification is: amount to 0.36g crude drug/g granule.
Embodiment 3
Preparation is produced in the arbitrary value ratio within the scope of Chinese prescription of the present invention:
(1) take by Chinese prescription: Fructus Ligustri Lucidi 800g, Radix Paeoniae Alba 2600g, Rhizoma Dioscoreae 1000g, Herba Epimedii 1900g, Rhizoma Curculiginis 800g, Semen Cuscutae 2200g, Caulis Polygoni Multiflori 700g, Fructus Rubi 2100g, Herba Leonuri 1100g, Radix Arnebiae (Radix Lithospermi) 1700g, Radix Rehmanniae 1000g, Radix Codonopsis 800g, Radix Astragali 2100g, Ramulus Uncariae Cum Uncis 1100g, Huai wheat 3100g, Semen Platycladi 600g, Rhizoma Polygonati Odorati 1200g, Radix Stephaniae Tetrandrae 500g, Radix Glycyrrhizae 1400g, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 8 times of weight, soak 16 hours, backflow decoction 3 hours, filter, then add the purified water of initial medical material 5 times of weight, backflow decoction 2 hours, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 75% of 6 times of weight, reflux, extract, 2 times, each 2 hours, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make granule, specification is: amount to 0.36g crude drug/g granule.
Embodiment 4
Preparation is produced in the ratio of Chinese prescription optimum ratio 1 of the present invention:
(1) take by Chinese prescription: Fructus Ligustri Lucidi 1200g, Radix Paeoniae Alba 2000g, Rhizoma Dioscoreae 1500g, Herba Epimedii 1400g, Rhizoma Curculiginis 1300g, Semen Cuscutae 1600g, Caulis Polygoni Multiflori 1200g, Fructus Rubi 1500g, Herba Leonuri 1700g, Radix Arnebiae (Radix Lithospermi) 1200g, Radix Rehmanniae 1600g, Radix Codonopsis 1300g, Radix Astragali 1500g, Ramulus Uncariae Cum Uncis 1600g, Huai wheat 2500g, Semen Platycladi 1000g, Rhizoma Polygonati Odorati 900g, Radix Stephaniae Tetrandrae 800g, Radix Glycyrrhizae 1100g, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 8 times of weight, soak 16 hours, backflow decoction 3 hours, filter, then add the purified water of initial medical material 5 times of weight, backflow decoction 2 hours, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 75% of 6 times of weight, reflux, extract, 2 times, each 2 hours, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make granule, specification is: amount to 0.36g crude drug/g granule.
Embodiment 5
Preparation is produced in the ratio of Chinese prescription optimum ratio 2 of the present invention:
(1) take by Chinese prescription: Fructus Ligustri Lucidi 1400g, Radix Paeoniae Alba 2100g, Rhizoma Dioscoreae 1600g, Herba Epimedii 1500g, Rhizoma Curculiginis 1200g, Semen Cuscutae 1500g, Caulis Polygoni Multiflori 1300g, Fructus Rubi 1700g, Herba Leonuri 1600g, Radix Arnebiae (Radix Lithospermi) 1300g, Radix Rehmanniae 1500g, Radix Codonopsis 1400g, Radix Astragali 1700g, Ramulus Uncariae Cum Uncis 1400g, Huai wheat 2700g, Semen Platycladi 1100g, Rhizoma Polygonati Odorati 1000g, Radix Stephaniae Tetrandrae 900g, Radix Glycyrrhizae 1200g, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 8 times of weight, soak 16 hours, backflow decoction 3 hours, filter, then add the purified water of initial medical material 5 times of weight, backflow decoction 2 hours, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 75% of 6 times of weight, reflux, extract, 2 times, each 2 hours, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make granule, specification is: amount to 0.36g crude drug/g granule.
Embodiment 6
Preparation is produced in the ratio of Chinese prescription optimum ratio 3 of the present invention:
(1) take by Chinese prescription: Fructus Ligustri Lucidi 1300g, Radix Paeoniae Alba 2200g, Rhizoma Dioscoreae 1400g, Herba Epimedii 1700g, Rhizoma Curculiginis 1500g, Semen Cuscutae 1400g, Caulis Polygoni Multiflori 1500g, Fructus Rubi 1600g, Herba Leonuri 1500g, Radix Arnebiae (Radix Lithospermi) 1300g, Radix Rehmanniae 1400g, Radix Codonopsis 1200g, Radix Astragali 1400g, Ramulus Uncariae Cum Uncis 1500g, Huai wheat 2400g, Semen Platycladi 1200g, Rhizoma Polygonati Odorati 800g, Radix Stephaniae Tetrandrae 900g, Radix Glycyrrhizae 1200g, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 8 times of weight, soak 16 hours, backflow decoction 3 hours, filter, then add the purified water of initial medical material 5 times of weight, backflow decoction 2 hours, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 75% of 6 times of weight, reflux, extract, 2 times, each 2 hours, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make granule, specification is: amount to 0.36g crude drug/g granule.
Embodiment 7
Preparation is produced in the ratio of Chinese prescription optimum ratio 4 of the present invention:
(1) take by Chinese prescription: Fructus Ligustri Lucidi 1400g, Radix Paeoniae Alba 2300g, Rhizoma Dioscoreae 1600g, Herba Epimedii 1600g, Rhizoma Curculiginis 1400g, Semen Cuscutae 1500g, Caulis Polygoni Multiflori 1400g, Fructus Rubi 1700g, Herba Leonuri 1800g, Radix Arnebiae (Radix Lithospermi) 1300g, Radix Rehmanniae 1500g, Radix Codonopsis 1400g, Radix Astragali 1600g, Ramulus Uncariae Cum Uncis 1700g, Huai wheat 2600g, Semen Platycladi 1100g, Rhizoma Polygonati Odorati 1100g, Radix Stephaniae Tetrandrae 900g, Radix Glycyrrhizae 1100g, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 8 times of weight, soak 16 hours, backflow decoction 3 hours, filter, then add the purified water of initial medical material 5 times of weight, backflow decoction 2 hours, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 75% of 6 times of weight, reflux, extract, 2 times, each 2 hours, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make granule, specification is: amount to 0.36g crude drug/g granule.
Embodiment 8
Animal toxicity test
(1) acute toxicity test
1, tested medicine: embodiment of the present invention 1-7 gained granule, adds purified water, make magma.
2, experimental animal: Kunming mouse, body weight 20g ± 4g, male and female half and half, female mice is all without pregnant.
3, the magma of mouse stomach Chinese medicine granules modulation, when given low reaches 749.6g crude drug/kg dosage, after administration, mice occurs that light activity reduces, 1.5 hours recover normal, Continuous Observation 7 days after administration, none animal dead, its overall health of patients, diet, take the photograph water, urine and body weight increase all normal.
4, result of the test shows: the maximum dosage-feeding of mouse stomach embodiment 1-7 gained granule is 749.6g crude drug/kg/d(LD 50> 749.6g crude drug/kg).Chinese medicine clinical application every day total amount of the present invention is 0.24g crude drug/kg/d; By weighing machine, the dosis tolerata of mouse stomach embodiment 1-7 gained granule is 3123.3 times of clinical patient.Point out this medicine acute toxicity extremely low, clinical drug safety.
(2) long term toxicity test
1, test objective: the toxic reaction of viewing test animal after edible Chinese medicine preparation of the present invention, assesses the basic security of Chinese medicine preparation of the present invention.
2, test material:
(1) animal: select adult healthy SD rat 240, original body mass 218 ± 11g, male and female half and half, female rats is all without pregnant.
(2) medicine: the granule produced by embodiment of the present invention 1-7, amounting to crude drug content is 0.36g crude drug/g granule, adds purified water, makes magma.Per weight dosage is by 20 times of calculating (clinical application amount foundation Pharmacopoeia of the People's Republic of China version in 2010) of clinical application amount.
3, test method
(1) 240 SD rats are divided into 8 groups, often organize 30, male and female half and half.Experimental animal male and female sub-cage rearing, 5, every cage, quantitative feeding (every cage 1300-1400g/ week), normal feedstuff is experimental mouse full-valence pellet feed, freely drinks water.Experimental temperature (21 ± 2) DEG C, humidity 60%-80%.The natural lighting light and shade cycle.After SD rat is bought, under this laboratory rearing condition, adapt to 2 weeks, observe general behavior performance.Test pre-test animal ingestion amount, selects qualified rat.
(2) medication
With the made magma of Chinese medicine granules of the present invention, to wherein 7 groups of SD rat oral gavages administration, every day 2 times, each volume is 0.2ml/10g body weight, successive administration 26 weeks.Remaining one group is matched group, by the normal saline of same method gavage equivalent.Experimental animal is weighed weekly 1 time, according to body weight change adjustment dosage.24h after administration in the 13rd week, often organize execution 10, male and female half and half, observe and detect indices; Remaining continuation administration was observed to 26 weeks, and 24h after last 1 administration, often organizes and put to death 10 again, male and female half and half, observe and detect indices; Remaining 10 are carried out 8 week convalescent period and observe.
(3) test item and the time
General inspection: the general status observing animal every day, as the mental status, dynamic response, behavioral activity, the change of dietary amount, defecation situation, eyes and duct secretions, hair color, cleannes etc.Body weight change situation and food ration measure weekly 1 time.
Hematological examination: after administration, the 13rd, 26 week and drug withdrawal recover the end of term (the 34th week) and detect following index respectively: packed cell volume (HCT), red blood cell count(RBC) (RBC), content of hemoglobin (HGB), numeration of leukocyte (WBC), lymphocyte comparative counting (LY), platelet count (PLT), neutrophilic granulocyte comparative counting (NE), mononuclear cell comparative counting (Mon) and clotting time (CT).
Blood parameters check: after administration the 13rd, 26 week and drug withdrawal recovery the end of term (the 34th week) detect following index respectively: alanine aminotransferase (ALT), alkali phosphatase (AST), Lomefloxacin aspartate injection (AST), total bilirubin (TB), albumin (ALB), gross protein (TP), blood glucose (GLU), blood urea nitrogen (BUN), T-CHOL (TC), creatinine (Crea), uric acid (UA), blood sodium (Na +), blood potassium (K +), blood calcium (Ca +) and β 2-microglobulin.
Urinate biochemical and urine routine: after administration, the 13rd, 26 week and drug withdrawal recover the end of term (the 34th week) each survey 1 time.Animal metabolism cage list cage is raised and is collected 24h urine, overnight fasting before test, detects urine bilirubin (BIL), glucose (GLU), ketoboidies (KET), protein, pH value, urobilinogen (URO), urine is occulted blood (BLO), nitrite (NIT), proportion and leukocyte (WBC); Quantitative determination of radioimmunoassay urine β 2-MG(renal tubular function obstacle sensitive indicator).
System postmortem and histopathologic examination: macroscopy is carried out to each internal organs, the organs such as heart, liver, lungs, kidney, spleen, brain, adrenal gland, thymus, thyroid, pancreas, uterus, ovary, testis, prostate are taken out and weighs, calculate organ coefficient (organ weights/body weight).With light microscopic, histological examination is carried out to above-mentioned internal organs and spinal cord, thymus, bladder, bone marrow (breastbone), duodenum, stomach, ileum, pancreas, hypophysis cerebri, optic nerve, lymph node, coton and rectal etc.
4, result of the test
The all rats of overview all do not show any abnormal conditions; Hematological examination, blood parameters inspection, urinate biochemical, urine routine, system postmortem and histopathologic examination, indices, compared with control animals, does not all have obvious difference.Therefore, Chinese medicine capsules of the present invention to SD rat without any toxic action, in view of 20 times of ratios of its behaviour dose, so can infer that to take be safe to Chinese medicine preparation of the present invention for patient.
Embodiment 9
The animal model test of pesticide effectiveness of Chinese medicine preparation of the present invention
1, test material
(1) experimental animal
SD rat, female; KM mice, female; There is provided by Shanghai Laboratory Animal Research Institute.
(2) test drug
Contrast medicine: female precious ball, the accurate word Z11020185 of traditional Chinese medicines, Beijing Tongrentang Technology Development Co.ltd. Pharmaceutical Factory.
The Chinese medicine granules produced in the arbitrary example of test medicine: embodiment of the present invention 4-7.
2, content of the test
(1) Chinese medicine preparation of the present invention is on the impact of castrated rats simulation female climacteric syndrome
(2) animal grouping, administration
Get 50 female rats to anaesthetize with pentobarbital sodium, abdominal part carries out abdominal part upward to be fixed, midaxillary line and apart from 2cm infall cropping outside spinal column from rat back most end rib, incision skin and dorsal muscles are about 1.5-2cm, ovary is extractd after the fallopian tube of ligation ovary lower end, postoperative by uterus angular convolution abdominal cavity, suture muscles and skin.Same method extracts opposite side ovary, and injection 3 days penicillins are in case infect continuously, within postoperative 5th day, starts vaginal smear and observes Rats During The Estrous Cycle, continuous 5 days, no longer occurs that the oestrous cycle (being all in diestrus) is for castration success with rat.Random packet is model group, matched group and the large, medium and small dosage group by reagent, 10/group, separately gets 10 female rats (not doing castration operation) as Normal group.Postoperative 9th day starts by the gastric infusion of dosage shown in table 1, wherein female precious ball is equivalent to 5 times of people's clinical application amount, the large, medium and small dosage of Chinese medicine composition of the present invention is equivalent to 10 times, 5 times, 2.5 times of people's clinical application amount respectively, matched group and model group gavage normal saline, every day 1 time, continuous 30 days.Each group of Rat Fast be can't help water 12h and is tested to next day.
(3) hormone serum level and hypothalamus detect neurotransmitter detection
Rat extracting blood separation of serum, detects E2, FSH, LH, β-EP content in serum to put method of exempting from, and detects GSH-PX, SOD, MDA activity and content; Put to death rat and take off thalamus rapidly, detect E, NA, DA, 5-HT content in neurotransmitter hypothalamus with efficient liquid phase-electrochemical detection method.
3, result of the test
(1) Chinese medicine preparation of the present invention is on the impact (see table 1) of hormone in Ovariectomized Rat Serum
Table 1 Chinese medicine preparation of the present invention on the impact (n=10) of hormone in Ovariectomized Rat Serum ( ± SD)
Note: compare with Normal group, * P < 0.05, * * P < 0.01, * * * P < 0.001; Compare with model group, △p < 0.05, △ △p < 0.01, △ △ △p < 0.001.
Result shows, castration model group rats compares serum estradiol (E2) content to be reduced with normal rats, follicle stimulating hormone (FSH), lutropin (LH) activity increases, and all has significant (respectively P < 0.05, P < 0.01, P < 0.01); Contrast medicine and Chinese medicine of the present invention large, medium and small dosage group rat E2 and Normal group comparing difference without significant; Chinese medicine preparation of the present invention big or middle dosage group rat FSH compares with matched group, active reduction, and difference has significant (respectively P < 0.01, P < 0.01); Contrast medicine and Chinese medicine preparation of the present invention large, medium and small dosage group rat LH compare with Normal group, activity increases, no significant difference (respectively P < 0.001, P < 0.05, P < 0.01, P < 0.01).Contrast medicine and Chinese medicine preparation of the present invention large, medium and small dosage group rat E2 compare with castration model group rats, and content increases, and no significant difference (equal P < 0.05); Chinese medicine preparation of the present invention large, medium and small dosage group rat FSH compares with model group rats, active reduction, and difference has significant (respectively P < 0.001, P < 0.001, P < 0.01); Chinese medicine preparation of the present invention large, medium and small dosage group rat LH compares with model group rats, and active reduction, difference has significant (equal P < 0.01).
In sum, test shows, Chinese medicine preparation of the present invention significantly can increase castration rat model E2 content, reduces FSH, LH active, has a better role to the imbalance of castration rat model hormonal system.
(2) Chinese medicine preparation of the present invention is on the impact (see table 2) of Ovariectomized Rat Serum free radical
Table 2 Chinese medicine preparation of the present invention on the impact (n=10) of Ovariectomized Rat Serum free radical ( ± SD)
Note: compare with model group, △p < 0.05.
Result shows, and castration rat model reduced glutathione (GSH-PX), superoxide dismutase (SOD) activity reduces, and malonaldehyde (MDA) content increases, but with matched group comparing difference without significant; Contrast medicine and Chinese medicine preparation of the present invention large, medium and small dosage GSH-PX, SOD, MDA, with matched group comparing difference without significant.Chinese medicine composition of the present invention large, medium and small dosage group compares with model group by rat, and GSH-PX activity increases, and difference all has significant (equal P < 0.05); In Chinese medicine composition of the present invention, dosage group rat is compared with model group, and SOD activity increases, and difference has significant (P < 0.05); Chinese medicine Chinese medicine preparation of the present invention large, medium and small dosage group compares with model group by rat, and MDA content reduces, no significant difference.
Test shows, it is active that Chinese medicine composition of the present invention can significantly improve castrated rats GSH-PX, in Chinese medicine composition of the present invention, dosage can significantly improve castrated rats SOD activity, Chinese medicine composition of the present invention can reduce castration rat model MDA content, has a better role to castration rat model Radical Metabolism.
Embodiment 10
The clinical trial of Chinese medicine preparation of the present invention
1, climacteric patient 200 example is collected in this clinical trial altogether, age 46-56 year, all experimenters are divided at random 5 groups, often organize 40 examples, be respectively matched group, test 1, test 2, test 3, test 4 groups, the oral female precious ball of matched group, every day 2 times, each 50, test 1-4 group is the Chinese medicine granules (amounting to 0.36g crude drug/g granule) produced of oral embodiment of the present invention 4-7 respectively, every day 2 times, each 20g.Within 4 weeks, be a course for the treatment of, treat 3 courses for the treatment of continuously.
2, judgment criteria
Cure: the symptomatology of climacteric syndrome disappears, and makes a definite diagnosis fully recover through doctor;
Effective: the partial symptoms of climacteric syndrome disappears, and the state of an illness is significantly improved;
Take a turn for the better: the partial symptoms of climacteric syndrome makes moderate progress;
Invalid: the symptom of climacteric syndrome is without any improvement, even more serious.
3, therapeutic outcome (see table 3)
Table 3 therapeutic outcome statistical analysis table
4, conclusion (of pressure testing)
Result of the test shows, the average cure rate of Chinese medicine preparation of the present invention is 37.5% higher than matched group 2.5 times, average effectiveness level is 94.4% higher than matched group 19.4 percentage points, therefore, relative to contrast medicine, the treatment of Chinese medicine granules of the present invention is with the obvious advantage, and Chinese medicine preparation of the present invention has very significant technical advantage relative to prior art, obtains beat all technological achievement.

Claims (8)

1. treat a Chinese medicine preparation for climacteric syndrome, it is characterized in that being made up of the raw material of following weight portion: Fructus Ligustri Lucidi 8-16 part, Radix Paeoniae Alba 14-26 part, Rhizoma Dioscoreae 10-20 part, Herba Epimedii 9-19 part, Rhizoma Curculiginis 8-18 part, Semen Cuscutae 10-22 part, Caulis Polygoni Multiflori 7-17 part, Fructus Rubi 9-21 part, Herba Leonuri 11-23 part, Radix Arnebiae (Radix Lithospermi) 7-17 part, Radix Rehmanniae 10-22 part, Radix Codonopsis 8-18 part, Radix Astragali 10-21 part, Ramulus Uncariae Cum Uncis 11-22 part, Huai wheat 19-31 part, Semen Platycladi 6-14 part, Rhizoma Polygonati Odorati 6-12 part, Radix Stephaniae Tetrandrae 5-11 part, Radix Glycyrrhizae 9-14 part.
2. the Chinese medicine preparation for the treatment of climacteric syndrome as claimed in claim 1, is characterized in that being made up of the raw material of following weight portion: Fructus Ligustri Lucidi 12 parts, the Radix Paeoniae Alba 20 parts, Rhizoma Dioscoreae 15 parts, Herba Epimedii 14 parts, Rhizoma Curculiginis 13 parts, Semen Cuscutae 16 parts, Caulis Polygoni Multiflori 12 parts, Fructus Rubi 15 parts, Herba Leonuri 17 parts, Radix Arnebiae (Radix Lithospermi) 12 parts, 16 parts, the Radix Rehmanniae, Radix Codonopsis 13 parts, the Radix Astragali 15 parts, Ramulus Uncariae Cum Uncis 16 parts, Huai wheat 25 parts, Semen Platycladi 10 parts, Rhizoma Polygonati Odorati 9 parts, Radix Stephaniae Tetrandrae 8 parts, 11 parts, Radix Glycyrrhizae.
3. the Chinese medicine preparation for the treatment of climacteric syndrome as claimed in claim 1, is characterized in that being made up of the raw material of following weight portion: Fructus Ligustri Lucidi 14 parts, the Radix Paeoniae Alba 21 parts, Rhizoma Dioscoreae 16 parts, Herba Epimedii 15 parts, Rhizoma Curculiginis 12 parts, Semen Cuscutae 15 parts, Caulis Polygoni Multiflori 13 parts, Fructus Rubi 17 parts, Herba Leonuri 16 parts, Radix Arnebiae (Radix Lithospermi) 13 parts, 15 parts, the Radix Rehmanniae, Radix Codonopsis 14 parts, the Radix Astragali 17 parts, Ramulus Uncariae Cum Uncis 14 parts, Huai wheat 27 parts, Semen Platycladi 11 parts, Rhizoma Polygonati Odorati 10 parts, Radix Stephaniae Tetrandrae 9 parts, 12 parts, Radix Glycyrrhizae.
4. the Chinese medicine preparation for the treatment of climacteric syndrome as claimed in claim 1, is characterized in that being made up of the raw material of following weight portion: Fructus Ligustri Lucidi 13 parts, the Radix Paeoniae Alba 22 parts, Rhizoma Dioscoreae 14 parts, Herba Epimedii 17 parts, Rhizoma Curculiginis 15 parts, Semen Cuscutae 14 parts, Caulis Polygoni Multiflori 15 parts, Fructus Rubi 16 parts, Herba Leonuri 15 parts, Radix Arnebiae (Radix Lithospermi) 13 parts, 14 parts, the Radix Rehmanniae, Radix Codonopsis 12 parts, the Radix Astragali 14 parts, Ramulus Uncariae Cum Uncis 15 parts, Huai wheat 24 parts, Semen Platycladi 12 parts, Rhizoma Polygonati Odorati 8 parts, Radix Stephaniae Tetrandrae 9 parts, 12 parts, Radix Glycyrrhizae.
5. the Chinese medicine preparation for the treatment of climacteric syndrome as claimed in claim 1, is characterized in that being made up of the raw material of following weight portion: Fructus Ligustri Lucidi 14 parts, the Radix Paeoniae Alba 23 parts, Rhizoma Dioscoreae 16 parts, Herba Epimedii 16 parts, Rhizoma Curculiginis 14 parts, Semen Cuscutae 15 parts, Caulis Polygoni Multiflori 14 parts, Fructus Rubi 17 parts, Herba Leonuri 18 parts, Radix Arnebiae (Radix Lithospermi) 13 parts, 15 parts, the Radix Rehmanniae, Radix Codonopsis 14 parts, the Radix Astragali 16 parts, Ramulus Uncariae Cum Uncis 17 parts, Huai wheat 26 parts, Semen Platycladi 11 parts, Rhizoma Polygonati Odorati 11 parts, Radix Stephaniae Tetrandrae 9 parts, 11 parts, Radix Glycyrrhizae.
6. prepare as arbitrary in claim 1-5 as described in the method for Chinese medicine preparation for the treatment of climacteric syndrome, it is characterized in that comprising following processing step:
(1) take ingredients by Chinese prescription, pulverize respectively, for subsequent use;
(2) all medical materials are added the purified water of initial medical material 6-10 times weight, soak 10-24 hour, backflow decocts 2-4 hour, filter, then add the purified water of initial medical material 3-7 times weight, backflow decocts 1-3 hour, filter, retain filtering residue, merging filtrate, being concentrated into 65 DEG C of relative densities is that the concentrated solution of 1.22-1.27 is for subsequent use;
(3) filtering residue of step (2) is added the ethanol of the concentration 65-80% of 4-7 times of weight, reflux, extract, 2 times, each 1-4 hour, filter, merging filtrate, distilling under reduced pressure removing ethanol, and to be concentrated into 65 DEG C of relative densities be that the concentrated solution of 1.22-1.27 is for subsequent use;
(4) merged by the concentrated solution of step (2) and (3) gained, continuing to be concentrated into 65 DEG C of relative densities is the Chinese medical concrete of 1.32-1.37, for subsequent use;
(5) Chinese medical concrete is added adjuvant, utilize modern general Chinese medicine preparation technology to make the finished dosage forms of current clinical needs.
7. the Chinese medicine preparation of the treatment climacteric syndrome as described in as arbitrary in claim 1-5, is characterized in that: described Chinese medicine preparation is tablet, pill, granule, capsule, powder, oral liquid or syrup.
8. the Chinese medicine preparation of the treatment climacteric syndrome as described in as arbitrary in claim 1-5, is characterized in that: described Chinese medicine preparation is used for the treatment that clinical manifestation is the disease of climacteric syndrome.
CN201510034669.7A 2015-01-23 2015-01-23 Traditional Chinese medicine preparation for treating climacteric syndrome and preparation method of traditional Chinese medicine preparation Withdrawn CN104547712A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105749232A (en) * 2016-04-07 2016-07-13 广州暨南生物医药研究开发基地有限公司 Folium artemisiae argyi-containing traditional Chinese medicine formula for tonifying kidney yang and nourishing kidney yin
CN106728649A (en) * 2017-01-12 2017-05-31 张超英 A kind of health pill for woman climacteric
CN113384673A (en) * 2021-06-17 2021-09-14 云南琼月企业管理有限公司 Qiyuantai pill Chinese medicine preparation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103191324A (en) * 2013-04-12 2013-07-10 杨邱月 Traditional Chinese medicine particle for treating climacteric syndrome and preparation method of traditional Chinese medicine particle

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103191324A (en) * 2013-04-12 2013-07-10 杨邱月 Traditional Chinese medicine particle for treating climacteric syndrome and preparation method of traditional Chinese medicine particle

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105749232A (en) * 2016-04-07 2016-07-13 广州暨南生物医药研究开发基地有限公司 Folium artemisiae argyi-containing traditional Chinese medicine formula for tonifying kidney yang and nourishing kidney yin
CN106728649A (en) * 2017-01-12 2017-05-31 张超英 A kind of health pill for woman climacteric
CN113384673A (en) * 2021-06-17 2021-09-14 云南琼月企业管理有限公司 Qiyuantai pill Chinese medicine preparation

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Application publication date: 20150429