CN104490979A - Application of rhodiola rosea extract in preparation of medicine resistant to adverse effects during radiotherapy and chemotherapy - Google Patents
Application of rhodiola rosea extract in preparation of medicine resistant to adverse effects during radiotherapy and chemotherapy Download PDFInfo
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Abstract
The invention relates to application of a rhodiola rosea extract in preparation of a medicine resistant to adverse effects during radiotherapy and chemotherapy. Rhodiola rosea is extracted by adopting alcohol and then is refined by virtue of macroporous adsorption resin, so that the rhodiola rosea extract is obtained; the rhodiola rosea extract mainly comprises glycoside and flavonoid ingredients, namely the following five ingredients: salidroside, p-tyrosol, rosavin, rosarin and total flavonoids; and a large number of tests show that the rhodiola rosea extract has an obvious therapeutic effect on a bone marrow suppression model and an immune inhibition model which are caused by radiotherapy and chemotherapy.
Description
Technical field
The present invention relates to the purposes of a kind of Radix Rhodiolae extract in the medicine of preparation opposing chemicotherapy untoward reaction, especially resist the purposes of bone marrow depression that chemicotherapy causes or immunological function repression untoward reaction.
Background technology
Malignant tumor is one of major disease of current serious harm people ' s health.The a data announced in July, 2008 according to World Health Organization (WHO) is pointed out: the number dying from cancer for 2007 reaches 7,900,000 people, accounts for 13% of total death toll.Dead major cancers kind is wherein caused to be: pulmonary carcinoma (1,400,000 people); Gastric cancer (86.6 ten thousand people); Hepatocarcinoma (65.3 ten thousand people); Colon cancer (67.7 ten thousand people) and breast carcinoma (54.8 ten thousand people).The death that the cancer of about 80% causes occurs in low income and middle income country.Estimate that continuation increases by the death toll that whole world cancer causes, the year two thousand thirty estimation will have 1,200 ten thousand people to die from cancer.At present, China's cancer year new cases are 2,000,000, and because number of cancer deaths is 1,400,000, China resident, often in dead 5 people, namely has 1 people to die from cancer.Cancer has become the No.1 cause of the death of China urbanite and the second largest cause of death of urban residents.
Radiotherapy chemotherapy utilizes anti-cancer chemical medicine or lonizing radiation to be treatment means, is the Main Means for the treatment of tumor at present, has good curative effect to kinds cancer.But when Radiotherapy chemotherapy is while killing and wounding cancerous cell, also normal cell non-selectivity is killed and wounded, be commonly referred to toxic and side effects of chemoradiotherapy or untoward reaction.Modal Radiotherapy chemotherapy untoward reaction has bone marrow depression, immunological function repression, gastrointestinal side effect, organ functional lesion, alopecia, skin lesion etc. clinically.In the untoward reaction that above-mentioned chemicotherapy causes, the most common with bone marrow depression, immunological function repression, have a strong impact on the quality of life of tumor patient, and treatment of cancer failure may have been caused, finally made patient's short survival.
Bone marrow depression refers to the activity decrease of the blood cell precursors in bone marrow.Erythrocyte in blood flow and leukocyte all come from the stem cell in bone marrow.The hemocyte life-span in blood flow is short, usually needs constantly to supplement.In order to reach the object of supplementing in time, the stem cell as blood cell precursors must division fast.Chemotherapy and radiotherapy and other tumor therapeuticing methods many, be all the cell for division fast, thus usually cause normal marrow cell suppressed.Bone marrow depression is the Common Toxicity reaction of most chemotherapeutic, and bone marrow depression not only delays the carrying out of chemotherapy and affects therapeutic effect, and may cause complication and jeopardize patient vitals.Some malignant tumor patients perhaps can not die from disease itself very soon, but may be fatal due to bone marrow depression.Most of chemotherapeutics all can cause bone marrow depression in various degree, and peripheral blood cells quantity is reduced.After bone marrow depression usually occurs in chemotherapy.Because the granulocyte mean survival time is the shortest, be about 6-8 hour, therefore bone marrow depression often shows as leukopenia at first; The platelet mean survival time is about 5-7 days, and its appearance that declines is more late lighter; And the erythrocyte mean survival time is 120 days, affect by chemotherapy less, decline usually not obvious.The bone marrow depression of most caused by chemotherapeutic medicines, after usually seeing chemotherapy, 1-3 is all, about continues to recover gradually in 2-4 week, and based on leukopenia, companion's platelet can be had to decline, and minority medicine such as gemcitabine, carboplatin, mitomycin etc. then drop to master with platelet.So leukocyte can be detected and hematoblastic quantity judges whether to there occurs bone marrow depression after chemotherapy.
Immunological function repression refers to the inhibit feature that foreign compound or other envirment factors produce body immune system, its main manifestations is: (1) suppresses nonspecific immunity, respiratory mucosa cilium Scavenging activity and macrophages phagocytic capacity can be made to decline, the antibiotic substance such as complement, lysozyme in body fluid is reduced, reduces Abwehrkraft des Koepers; (2) suppress specific immunity, cause lymphatic organ atrophy, peripheral lymphocyte reduce and function inhibitio, antibody formed suppress; (3) Immunosuppression function for monitoring.Cancer patient body's immunity is in obvious disturbance state, loses the monitoring capacity to cancerous cell, and part cancerous cell being regarded as self of mistake, and allow cancerous cell continued propagation and fast breeder in vivo.Meanwhile, the immunologic function of Radiotherapy chemotherapy further destroyed tumor patient again, causes its immunologic function to decline further, and causes serious infection thus, impel state of an illness Progressive symmetric erythrokeratodermia increase the weight of or worsen.
The clinical medicine for bone marrow depression caused by Radiotherapy chemotherapy, immunological function repression symptomatic treatment has: recombined human granulocyte stimulating factors (G-CSF) injection, fragrant Fu oral liquid for benefiting blood, ZHENQI FUZHENG KELI, matrine injection etc.
Radix Rhodiolae is the dry root welding technology of crassulaceae plants Radix Rhodiolae Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba, belong to natural precious medicinal plants, be described as " plateau Radix Ginseng ", " snow mountain Herba mesonae chinensis ", widely use among the people as strong good medicine since ancient times.All detailed introduction is had to Radix Rhodiolae to the pharmacopeia in modern times from " legendary god of farming book on Chinese herbal medicine " of Ancient Times in China, Compendium of Material Medica.The traditional Chinese medical science thinks that its property is sweet, bitter, flat, return lung, heart channel, there is benefiting QI for activating blood circulation, promote blood circulation relieving asthma, the effect such as strengthening the body resistance, for blood stasis due to qi deficiency, obstruction of qi in the chest and cardialgia, apoplectic hemiplegia, asthenia asthma.Modern 20 century 70s are started to its research, the former Russian scholar is decided to be " adaptogen " material, and report that there is multiple pharmacological effect.China and Russian scholar conduct in-depth research it in recent years, and under Radix Rhodiolae is the mal-condition of growth between alpine meadow and cheuch, the tundra band wild plant that natural climate is changeable, its vitality is strong, has the biological activity of many uniquenesses.
Radix Rhodiolae is characteristic resources and the superior resources in Xinjiang, be distributed in the high and cold free of contamination Mountain Ranges of Tian Shan Mountains, rich reserves, medication among the people is with a long history, has enhancing immunity, defying age, resisting fatigue, anti-hypoxia, anti-pessimal stimulation, antiviral, radioprotective, improves many-sided pharmacological actions such as cardio-cerebrovascular function, antitumor.Have wide range of applications, side effect is little.But about Radix Rhodiolae extract as the untoward reaction that causes of preparation opposing chemicotherapy medicinal usage there is not been reported.
The extracting method of Radix Rhodiolae is a lot, adopts the method for water extract-alcohol precipitation can obtain Radix Rhodiolae polysaccharide constituents; After alcohol extraction, macroporous resin is refining can obtain the composition such as flavonoid, glucosides class, then through silica gel column chromatography, chloroform-methanol eluting is separable obtains the monomer components such as rhodioside, butyl alcohol, cheese thiophene dimension, cheese thiophene beautiful jade; Also means such as adopting ultrasound wave, microwave, enzymolysis, ultrafiltration are had to extract Radix Rhodiolae.
Summary of the invention
The object of the invention is, a kind of Radix Rhodiolae extract is provided to resist the purposes in the medicine of the untoward reaction that chemicotherapy causes in preparation, after Radix Rhodiolae being adopted alcohol extraction, the method for Macroporous Adsorption Resin can obtain Radix Rhodiolae extract, this extract mainly comprises glycoside, flavones ingredient, it mainly comprises rhodioside (salidroside), butyl alcohol (p-tyrosol), cheese thiophene dimension (rosavin), cheese thiophene beautiful jade (rosarin) and total flavones (total flavonoids) 5 kinds of compositions, through a large number of experiments show that: this extract is to bone marrow depression model, immunosuppression model has therapeutical effect significantly, there is opposing put, bone marrow depression caused by chemotherapy, the drug action of immunological function repression untoward reaction.
The purposes of a kind of Radix Rhodiolae extract of the present invention, the purposes of this Radix Rhodiolae extract in the medicine of the untoward reaction caused by preparation opposing Radiotherapy chemotherapy.
Untoward reaction caused by described opposing Radiotherapy chemotherapy is the untoward reaction of bone marrow depression untoward reaction or immunological function repression.
Radix Rhodiolae extract of the present invention resists the purposes in the medicine of the untoward reaction that chemicotherapy causes in preparation, to the anti-bone marrow inhibition of Radix Rhodiolae extract, comparatively systematic research has been carried out in anti-immunological function repression effect: (1) is research Radix Rhodiolae extract causes mouse bone marrow cells inhibition effect to radiotherapy, mouse bone marrow cells inhibition is set up through the disposable irradiation of 60Co gamma-rays, prove after modeling success through index checkings such as routine blood tests, give Radix Rhodiolae extract various dose to show: Radix Rhodiolae extract reduces not obviously short restitution to the thymus coefficient of radiotherapy mice and Spleen coefficient, but leukocyte and platelet reduction in Mouse Blood are caused to radiotherapy there is obviously short restitution, (2) for research Radix Rhodiolae extract causes the effect of tumor-bearing mice bone marrow depression model to radiotherapy, to healthy Kunming mouse inoculation S180 sarcoma cell, set up S180 sarcoma tumor-bearing mice, tumor-bearing mice radiotherapy bone marrow depression model is set up through the disposable irradiation of 60Co gamma-rays, prove after modeling success through index checkings such as routine blood tests, give Radix Rhodiolae extract to carry out treatment and show: the tumor control rate of Radix Rhodiolae extract to radiotherapy tumor-bearing mice does not have obvious effect, but causing leukocyte and platelet in tumor-bearing mice blood to radiotherapy reduces and has obviously short restitution, (3) for research Radix Rhodiolae extract causes the effect of tumor-bearing mice immunosuppression model to chemotherapy, CD8 is utilized
+detection kit, IgG detection kit and lysozyme detection kit detect Radix Rhodiolae extract causes tumor-bearing mice immunosuppression model effect to chemotherapy, and result shows: the CD8 of tumor-bearing mice
+, IgG and LZM significantly reduce (P<0.05).Radix Rhodiolae extract has promotion CD8
+the effect expressed, can return to normal level.Radix Rhodiolae extract has the effect of IgG content in increasing serum, and can promote the growth of lysozyme content in animal serum.(4) for the main component of research Radix Rhodiolae is to the potentiation of mouse immune system, 3 main component rhodiosides in Radix Rhodiolae are carried out, butyl alcohol, cheese thiophene dimension is to mice T, the Effect study of bone-marrow-derived lymphocyte, the cytophagic influence research of dialogue and the Effect study to mouse monokaryon macrophage, result shows: rhodioside is when high concentration (250 μ g/ml), the lymphocytic conversion ratio of T/B and the rate of increase can not only be promoted, also the phagocytic activity of Number of Peripheral Blood Leucocyte and peritoneal macrophage has significant castering action, show that rhodioside is very extensive to the immunization of mouse immune system, panimmunity responsing reaction can be participated in, another main component butyl alcohol of Radix Rhodiolae (62.5 μ g/ml-250 μ g/ml) then can the propagation of specific stimulation bone-marrow-derived lymphocyte and conversion (P≤0.05), and action effect is higher than positive drug group, this shows that butyl alcohol specificly can act on humoral immunoresponse(HI) system, cheese plug dimension then has significant facilitation (p≤0.05) to silent oscillation T cell to blast transformation.Illustrate that Radix Rhodiolae three kinds of main component rhodiosides, butyl alcohol, cheese thiophene dimension has and promote cell and humoral immune function, and heterogeneity acts on different immune target cells.(5) with Radix Rhodiolae main component rhodioside, butyl alcohol, cheese thiophene dimension, cheese thiophene beautiful jade and total flavones for inspection target, screening test is carried out to extraction process, result shows: through Macroporous Adsorption Resin after Radix Rhodiolae alcohol extraction, dry extract yield is greater than 15%, 5 kinds of component contents are greater than 50%, illustrate after adopting alcohol extraction and macroporous resin to refine, can the rhodioside of enrichment preferably, butyl alcohol, cheese thiophene dimension, cheese thiophene beautiful jade and these 5 kinds of main components of total flavones.
Accompanying drawing explanation
Fig. 1 is GIEMSA dyeing after the induction T/B lymphocyte transformation 24h such as rhodioside of the present invention, (100 ×) figure, wherein arrows: A: no transformed cells: the minimum about 6-8 μm of volume, cell is without expanding, and chromatin is intensive, without kernel, invisible mitotic event is painted the most shallow; B: transitional-cell: the medium 12-16 μm of volume, cell expands, and chromatin loosens, and has 1 or do not have the painted more shallow C of kernel: blast cell: the most about 12-20 μm of volume, and chromatin loosens, and has 1-4 core, painted the darkest.
Detailed description of the invention
Embodiment
Radix Rhodiolae extract of the present invention resists the purposes in the medicine of the untoward reaction that chemicotherapy causes in preparation, after adopting alcohol extraction, the method for Macroporous Adsorption Resin can obtain Radix Rhodiolae extract, this extract mainly comprises glycoside, flavones ingredient, it is mainly rhodioside (salidroside), butyl alcohol (p-tyrosol), cheese thiophene dimension (rosavin), cheese thiophene beautiful jade (rosarin) and total flavones (total flavonoids) 5 kinds of compositions, and the Hydrolysis kinetics method of this Radix Rhodiolae extract follows these steps to carry out:
The Extractive technology of Radix Rhodiolae extract: get dry Radix Rhodiolae pulverizing medicinal materials, crosses No. 2 sieves; Take 500g medicinal powder, adding concentration is 5%-95% alcohol reflux 1-3 time, and the solvent amount added is 8-20 times, extracts 1-3h, and it is 0.8-1.2 that extracting liquid filtering, merging are concentrated into relative density;
Resin model is adopted to be the macroporous adsorbent resin of LS-300B, D101, AB-8, HPD700, loading relative density of medicine liquid is 0.8-1.2, applied sample amount is 0.3BV-0.8BV, loading flow velocity 1BV/h-3BV/h, adsorption time 60min, first uses purified water eluting, and consumption is 1-4BV, elution flow rate is 1BV/h-3BV/h, and eluent discards;
Be 20%-80% ethanol elution by concentration again, consumption is 1-4BV, and eluent retains, concentrated, and dry, baking temperature 40-90 DEG C, obtains Radix Rhodiolae extract, and extract yield is 15-35%;
Employing UV method, HPLC method carry out assay to Radix Rhodiolae extract, and the content summation recording the rhodioside in Radix Rhodiolae extract, butyl alcohol, cheese thiophene dimension, cheese thiophene beautiful jade and total flavones is greater than 50%.
The purposes of Radix Rhodiolae extract in the medicine of preparation opposing chemicotherapy untoward reaction:
Effect experiment observation is carried out to Radix Rhodiolae extract:
Radix Rhodiolae extract causes the research of mouse bone marrow cells inhibitory action to radiotherapy
Warp
60mouse bone marrow cells inhibition is set up in the disposable irradiation of Co gamma-rays, give Radix Rhodiolae extract various dose to show, Radix Rhodiolae extract causes leukocyte and platelet in Mouse Blood to radiotherapy to be reduced and has obviously short restitution, reduces obviously short restitution to the thymus coefficient of radiotherapy mice and Spleen coefficient;
Instrument and material:
Instrument:
Microscope (Chongqing photoelectric instrument, XDS-1B), electronic balance (sky inspires confidence in board electronic counting balance), syringe, gastric perfusion needle, gets blood vessel;
Sample and reagent and preparation:
Radix Rhodiolae extract (Hua Shidan institute of materia medica provides by Xinjiang), levamisole (Shandong Kernel and Hall Pharmaceutical Industry Co., Ltd., lot number: 130505).
Animal Kunming mouse, male, Animal Experimental Study center, Xinjiang provides, SCXK (newly) 2011-0001, body weight 18-22g, age 6-8 week.
Experimental technique:
Model is set up:
Mice adopts
60the disposable irradiation of Co gamma-rays, exposure dose 4.0Gy (close rate 0.63Rad/s, irradiation time 640s, irradiation distance 200cm), bone marrow depression model is set up in disposable irradiation, after irradiation 24h, to mice random packet according to administration.
Experiment grouping and dosage regimen:
Get 10 as Normal group, 60 model mouses are divided into 6 groups, be respectively dosage combination radiotherapy group and Radix Rhodiolae extract High dose radiotherapy group in model control group, model combination radiotherapy group, positive control medicine combination radiotherapy group, Radix Rhodiolae extract low-dose radiotherapy group, Radix Rhodiolae extract, often organize 10, normal group and model group give normal saline, 0.2ml/10g, 1 time/d, continuous 7d; Positive drug group gavage levamisole, 0.2ml/10g, 1 time/d, continuous 7d; Radix Rhodiolae extract gavage, 0.2ml/10g, 1 time/d, continuous 7d.Concrete grouping and process are in table 1;
Table 1 tests grouping and process
Fasting after last administration, can't help water 12h after, put to death all mouse weights simultaneously and detect corresponding index, record and take statistics learn with broken line graph analysis.
Testing index:
The detection of WBC, RBC, HGB, PLT in blood:
Orbital venous plexus gets blood, detects numeration of leukocyte (WBC), red blood cell count(RBC) (RBC), hemoglobin (HGB) and platelet count (PLT).
Organ index:
Mice takes thymus and spleen, and filter paper sucks surperficial residual blood, weighs on electronic balance, calculates organ coefficient (organ coefficient of every 100g body weight);
Organ coefficient=organ weights (g)/body weight (g) * 100.
Frangments smear:
Cervical dislocation puts to death each mice, take out femur, reject muscle and connective tissue, cut off femur two ends, No. 6 syringe needle normal saline lml/ prop up and go out medullary cell, No. 4 syringe needles filter makes single bone marrow cell suspension, extracting marrow cell suspension, and full-automatic blood counting instrument measures bone marrow nucleated cell (BMC) value.
Experimental result and analysis:
The impact of body weight:
This tests employing
60the disposable irradiation of Co gamma-rays, absorbed dose are 4.0Gy modeling, have certain influence to Mouse Weight, but there is no remarkable effect (P > 0.05); Each group of Mice Body weight average is in rising trend, and the low extract of Radix Rhodiolae declines to the Mouse Weight that radiation is shone certain antagonism.And the body weight of the 7th day with blank group than variant (P < 0.05) table 2;
Table 2 Mouse Weight result of variations (x ± SD)
*p<0.05,
*p<0.01Vs normal group;
#p<0.05,
##p<0.01Vs model group
3.2 impacts on organ coefficient:
From result,
60co gamma-rays, the disposable irradiation mice of 4.0GY exposure dose has a significant effect (p < 0.01) to its organ coefficient, and each dosage group of Radix Rhodiolae extract causes the reduction of mice organs coefficient to radiotherapy and is not significantly improved, table 3;
Table 3 is on the impact of organ coefficient
Note: compare " * " with negative control group and represent P<0.05, " * * " represents P<0.01;
Compare " # " with model control group and represent P<0.05, " ## " represents P<0.01.
Impact on blood WBC, RBC, HGB, PLT:
From result,
60co gamma-rays 4.0Gy absorbed dose disposable irradiation mice has remarkable reducing effect (p < 0.01) to WBC, RBC, HGB, PLT in its peripheral blood.Positive drug levamisole can promote radiotherapy mouse blood WBC and hematoblastic content.Radix Rhodiolae extract has promotion restitution, in table 4 to leukocyte and platelet in radiotherapy mouse blood;
Table 4 is on the impact of blood WBC, RBC, Hb, PLT
Note: compare " * " with negative control group and represent P<0.05, " * * " represents P<0.01;
Compare " # " with model control group and represent P<0.05, " ## " represents P<0.01.
Impact on bone marrow nucleated cell:
From result,
60co gamma-rays disposable irradiation 4.0GY absorbed dose, have no significant effect (p > 0.05) mouse femur bonemarrow nucleated cells number.The each dosage group of Radix Rhodiolae extract causes the reduction of mouse femur bonemarrow nucleated cells number to radiotherapy and does not have a significant effect, in table 5;
Table 5 is on the impact of bone marrow nucleated cell
Note: compare " * " with negative control group and represent P<0.05, " * * " represents P<0.01;
Compare " # " with model control group and represent P<0.05, " ## " represents P<0.01.
Conclusion:
To sum up, this test
60co gamma-rays, 4.0GY absorbed dose disposable irradiation mice can cause the typical bone marrow depression model of mice.Levamisole can promote the recovery of leukocyte and platelet content in radiotherapy mouse blood, Radix Rhodiolae extract causes leukocyte and platelet in Mouse Blood to radiotherapy to be reduced and has obviously short restitution, reduces obviously short restitution to the thymus coefficient of radiotherapy mice and Spleen coefficient.
Radix Rhodiolae extract causes the research of tumor-bearing mice bone marrow inhibition to radiotherapy:
To healthy Kunming mouse inoculation S180 sarcoma cell, set up S180 sarcoma tumor-bearing mice, warp
60tumor-bearing mice radiotherapy bone marrow depression model is set up in the disposable irradiation of Co gamma-rays, after giving Radix Rhodiolae extract various dose, find, the tumor control rate of Radix Rhodiolae extract to radiotherapy tumor-bearing mice does not have obvious effect, cause leukocyte and platelet in tumor-bearing mice blood to radiotherapy to reduce there is obviously short restitution, obviously short restitution is reduced to the thymus coefficient of radiotherapy tumor-bearing mice and Spleen coefficient.
Experiment material:
Laboratory animal: Kunming mouse, male, Animal Experimental Study center, Xinjiang provides, SCXK (newly) 2011-0001, body weight 18-22g, age 6-8 week.
Experiment reagent: Radix Rhodiolae extract (be called for short extract, Hua Shidan institute of materia medica provides by Xinjiang), levamisole.
Test tumor: S180 sarcoma: Xinjiang Huashidan Medicine Research Co., Ltd goes down to posterity maintenance.
Experimental technique:
Model is set up: under aseptic condition, prepare tumor cell suspension, cell concentration is about 10
7individual cell/ml, gets healthy mice, and right front toe axil subcutaneous vaccination tumor cell 0.2ml/ only, sets up bearing mouse model, after 3 days, adopted by tumor-bearing mice
60bone marrow depression model is set up in the disposable irradiation of Co gamma-rays, exposure dose 4.0Gy (close rate 0.63Rad/s, irradiation time 640s, irradiation distance 200cm), after irradiation 24h, to tumor-bearing mice random packet according to administration.
Experiment grouping:
Get 10 as Normal group, 60 lotus tumor model mouses are divided into 6 groups, be respectively dosage combination radiotherapy group, Radix Rhodiolae extract High dose radiotherapy group in model control group, model combination radiotherapy group, positive control medicine combination radiotherapy group, Radix Rhodiolae extract low-dose radiotherapy group, Radix Rhodiolae extract, often organize 10, normal group and model group give normal saline, 0.2ml/10g, 1 time/d, continuous 7d; Positive drug group gavage levamisole, 0.2ml/10g, 1 time/d, continuous 7d; Extract gavage, 0.2ml/10g, 1 time/d, continuous 7d, concrete grouping and process are in table 6:
Table 6 tests grouping and process
Fasting after last administration, can't help water 12h after, put to death all mouse weights simultaneously and detect corresponding index, record and take statistics learn with broken line graph analysis.
Testing index:
Tumour inhibiting rate calculates: take tumor, weigh, and calculates tumour inhibiting rate;
The average tumor of tumour inhibiting rate %=(model control group average tumor weight-combination radiotherapy group average tumor weight)/model control group heavy × 100%.
Detect routine blood test: orbital venous plexus gets blood, detect numeration of leukocyte (WBC), red blood cell count(RBC) (RBC), hemoglobin (Hb) and platelet count (PLT).
Organ index: take thymus and spleen, filter paper sucks surperficial residual blood, weighs on electronic balance, organ weight index=organ weight (mg)/body constitution heavy (g).
Frangments smear: cervical dislocation puts to death each mice, take out femur, reject muscle and connective tissue, cut off femur two ends, No. 6 syringe needle normal saline lml/ prop up and go out medullary cell, No. 4 syringe needles filter makes single bone marrow cell suspension, extracting marrow cell suspension, and full-automatic blood counting instrument measures bone marrow nucleated cell (BMC) value.
Result and analysis:
Impact on mice tumour inhibiting rate:
Result shows, and radiotherapy has certain Developing restraint effect to S180 sarcoma mouse tumor, and Radix Rhodiolae extract is to not demonstrating obvious Tumor suppression growth.
Table 7 Radix Rhodiolae extract is to S180 sarcoma mice irradiation model tumor-inhibiting action
Note: "+" compares p<0.05 with model combination radiotherapy group, " ++ " compares p<0.01 with model combination radiotherapy group.
Detect routine blood test:
From result, healthy mice, after inoculation S180 sarcoma, leukocyte obviously declines (p<0.05), and hemoglobin obviously reduces (p<0.05), mice with tumor warp
60after the disposable irradiation of Co gamma-rays, leukocyte, erythrocyte, hemoglobin and platelet significantly decline (p<0.01), and the basic, normal, high dosage of Radix Rhodiolae extract causes the leukocyte of mice with tumor and platelet to radiotherapy to be reduced and have significant short restitution (p<0.05).
Table 8 Radix Rhodiolae extract is on the impact of S180 sarcoma mice irradiation model Mouse Blood routine
Note: " * " compares p<0.05 with normal group, " * * " compares p<0.01 with normal group, " # " compares p<0.05 with the blank group of model, " ## " compares p<0.01 with the blank group of model, "+" compares p<0.05 with model combination radiotherapy group, and " ++ " compares p<0.01 with model combination radiotherapy group.
Organ index:
Result shows, S180 sarcoma does not affect (P>0.05) mouse thymus and Spleen coefficient, after bearing animals radiotherapy, thymus and Spleen coefficient significantly decline (P<0.05), and Radix Rhodiolae extract declines to the thymus of tumor in radiotherapy mice and Spleen coefficient does not have restitution.
Table 9 Radix Rhodiolae extract is on the impact of S180 sarcoma mice irradiation model mice organs coefficient
Note: " * " compares p<0.05 with normal group, " * * " compares p<0.01 with normal group, " # " compares p<0.05 with the blank group of model, " ## " compares p<0.01 with the blank group of model, "+" compares p<0.05 with model combination radiotherapy group, and " ++ " compares p<0.01 with model combination radiotherapy group.
Frangments smear:
Result shows, S180 sarcoma does not affect (P>0.05) mouse bone marrow cells nucleated cell quantity, after bearing animals radiotherapy, bonemarrow nucleated cells number amount significantly raises (P<0.05), and the bonemarrow nucleated cells number amount of Radix Rhodiolae extract on tumor in radiotherapy mice does not affect.
Table 10 Radix Rhodiolae extract is on the impact of S180 sarcoma mice irradiation model mouse bone marrow cells nucleated cell quantity
Note: " * " compares p<0.05 with normal group, " * * " compares p<0.01 with normal group, " # " compares p<0.05 with the blank group of model, " ## " compares p<0.01 with the blank group of model, "+" compares p<0.05 with model combination radiotherapy group, and " ++ " compares p<0.01 with model combination radiotherapy group.
Conclusion:
To healthy Kunming mouse inoculation S180 sarcoma cell, set up S180 sarcoma tumor-bearing mice, warp
60tumor-bearing mice radiotherapy bone marrow depression model is set up in the disposable irradiation of Co gamma-rays.
Tumor-bearing mice compares with normal mouse, mice with tumor leukocyte obviously increases (P<0.05), erythrocyte obviously reduces (P<0.05), thymus coefficient and Spleen coefficient do not have notable difference, have certain reducing effect to bonemarrow nucleated cells number amount.
Tumor-bearing mice warp
60after the disposable irradiation of Co gamma-rays (4.0Gy), compared with mice with tumor, leukocyte, erythrocyte, hemoglobin and platelet significantly decline (p<0.01), thymus coefficient obviously reduces (P<0.05), Spleen coefficient significantly declines (p<0.01), does not have obvious effect to bonemarrow nucleated cells number amount.
After giving Radix Rhodiolae extract various dose, the tumor control rate of Radix Rhodiolae extract to radiotherapy tumor-bearing mice does not have obvious effect, cause leukocyte and platelet in tumor-bearing mice blood to radiotherapy to reduce there is obviously short restitution, obviously short restitution is reduced to the thymus coefficient of radiotherapy tumor-bearing mice and Spleen coefficient.
Radix Rhodiolae extract causes tumor-bearing mice immunosuppression model Effect study to chemotherapy:
This experiment is main is object of study with Radix Rhodiolae extract, utilizes CD8
+detection kit, IgG detection kit and lysozyme detection kit detect Radix Rhodiolae extract causes tumor-bearing mice immunosuppression model effect to chemotherapy, and result of study shows: the CD8 of tumor-bearing mice
+, IgG and LZM significantly reduce (P<0.05).Radix Rhodiolae extract has promotion CD8
+the effect expressed, can return to normal level.Radix Rhodiolae extract has the effect of IgG content in increasing serum, and promotes the growth of lysozyme content in animal serum, but can not return to normal level.Radix Rhodiolae extract has no significant effect mice with tumor thymus and Spleen coefficient.
Experiment material:
Laboratory animal: Kunming mouse, male, Animal Experimental Study center, Xinjiang provides, SCXK (newly) 2011-0001, body weight 18 ~ 22g, 6 ~ 8 weeks ages.
Experiment reagent: Radix Rhodiolae extract; Tri-distilled water; Syringe; Gastric perfusion needle; IVC system; CD8
+detection kit, immunoglobulin IgG detection kit and lysozyme detection kit; Modeling medicine: cyclophosphamide medicine; Positive drug: levamisole (Shandong Kernel and Hall Pharmaceutical Industry Co., Ltd., lot number: 130505).
Test tumor: S180 sarcoma: Xinjiang Huashidan Medicine Research Co., Ltd goes down to posterity maintenance.
Experimental technique:
Model is set up: under aseptic condition, prepare tumor cell suspension, cell concentration is about 10
7individual cell/ml, gets healthy mice, and only, set up bearing mouse model, tumor-bearing mice intraperitoneal injection of cyclophosphamide, dosage is 80mg/kg to right front toe axil subcutaneous vaccination tumor cell 0.2ml/, and injection causes tumor-bearing mice immunodeficiency models in 3 days continuously.
Grouping: get 10 mices as Normal group, 60 lotus tumor model mouses are divided into 6 groups, be respectively dosage group, Radix Rhodiolae extract high dose group in negative control group, model control group, positive drug levamisole matched group, Radix Rhodiolae extract low dose group, Radix Rhodiolae extract, often organize 10, specifically divide into groups in table 2.Normal group and negative control group are normally raised, and every day gavages purified water, and all the other each group gavaged on the basis of a sample at the 1st day to the 3rd day, intraperitoneal injection of cyclophosphamide; After last administration after fasting 8h, put to death all mouse weights simultaneously and detect corresponding index, recording and take statistics and learn and broken line graph analytical table 11;
Table 11 Radix Rhodiolae extract is to the processing mode of kunming mice and grouping
Testing index:
Tumour inhibiting rate calculates: take tumor, weigh, and calculates tumour inhibiting rate;
The average tumor of tumour inhibiting rate %=(model control group average tumor weight-combination radiotherapy group average tumor weight)/model control group heavy × 100%.
T lymphocyte subset group, immunoglobulin IgG and lysozyme content measure: extract eyeball blood sampling, the blood got is in the centrifugal 10min of 4000r/min, and separation of serum, uses CD8 respectively
+, immunoglobulin IgG test kit and lysozyme test kit description measure change of serum C D8
+, IgG and lysozyme content.
Organ index: cervical dislocation puts to death mice, and take thymus, spleen, filter paper sucks surperficial residual blood, weighs on electronic balance, organ weight's index=organ weight (g)/body weight (g).
Results and analysis:
Impact on tumor: result shows, and chemotherapy has S180 sarcoma mouse tumor and has significant Developing restraint effect (P<0.01), and Radix Rhodiolae extract is to not demonstrating obvious Tumor suppression growth table 12;
Table 12 Radix Rhodiolae extract is to S180 sarcoma mouse chemotherapy model tumor-inhibiting action
Note: " * * " compares P<0.01 with model control group.
T lymphocyte subset group, immunoglobulin IgG and lysozyme content measure:
By to the research of Radix Rhodiolae extract to the effect of chemotherapy tumor-bearing mice immunosuppression model, utilize CD8 respectively
+, CD8 in immunoglobulin IgG test kit and lysozyme kit measurement different experiments mice serum
+, IgG and lysozyme content, to understanding Radix Rhodiolae extract to the effect table 13 causing tumor-bearing mice immunosuppression model;
Table 13 CD8
+, IgG and lysozyme test kit testing result
Note:
*p<0.05,
*p<0.01Vs normal group
#p<0.05,
##p<0.01Vs model group
CD8
+the testing result of test kit: CD8
+be the lymphocytic subgroup of T, killing and wounding the important function of virus infected cell and tumor cell, model group is relative to CD8 in its serum of normal group as shown in Table 3
+content reduce, and reach significant level (P<0.05), and the middle and high dosage group of levamisole group, extract CD8 in its serum compared with model group
+content has remarkable growth (P<0.05).
The testing result of IgG test kit: IgG is the representational antibody of most in immunity system immunoglobulin, and the height of its content can reflect immune state and the function of host to a certain extent.As shown in Table 3, negative group and model group reduce relative to the content of IgG in its serum of normal group, and reach significance level (P<0.05), levamisole group can significantly improve IgG content in serum (P<0.05), and the basic, normal, high dosage group of Radix Rhodiolae extract can significantly improve IgG content in serum (P<0.05).
The testing result of LZM (lysozyme) test kit: lysozyme is the ingredient of normal body immune defence mechanism; this experiment is by the content of lysozyme in determination experiment mice serum, and understanding Radix Rhodiolae extract and Rhodiola root oral liquid are on the impact of immunity of organism defense mechanism.Known by table 3, significance in the serum of the laboratory animal of inoculated tumour cell reduces (negative control group), by feeding, antitumor drug cyclophosphamide (model control group) can reduce lysozyme content in serum further, levamisole can reduce by the lysozyme content that causes of antagonism cyclophosphamide, and the basic, normal, high dosage group of Radix Rhodiolae extract can improve the content of lysozyme in experiment mice serum.
Organ index: result shows, S180 sarcoma does not affect (P>0.05) mouse thymus and Spleen coefficient, after bearing animals chemotherapy, Spleen coefficient significantly raises (P<0.01), and Radix Rhodiolae extract does not have obvious effect table 14 to the thymus of tumor in radiotherapy mice and Spleen coefficient;
Table 14 Radix Rhodiolae extract is on the impact of S180 sarcoma mouse chemotherapy model mice organ coefficient
Note: " * " compares P<0.05 with model control group, " # " compares p<0.05 with the blank group of model, and " ## " compares p<0.01 with normal group.
Conclusion: to healthy Kunming mouse inoculation S180 sarcoma cell, set up S180 sarcoma tumor-bearing mice, through lumbar injection High-Dose Cyclophosphamide, set up tumor-bearing mice chemotherapeutic immunity inhibition.Tumor-bearing mice compares with normal mouse, mice with tumor CD8
+, IgG and LZM significantly reduce (P<0.05).Radix Rhodiolae extract has promotion CD8
+the effect expressed, can return to normal level.Radix Rhodiolae extract has the effect of IgG content in increasing serum, but can not return to normal level.Radix Rhodiolae extract can promote the growth of lysozyme content in animal serum, but can not return to normal level.
Radix Rhodiolae main component is studied the potentiation of mouse immune system:
Experiment material and instrument
Medicine and reagent: rhodioside, tyrosol, cheese thiophene tie up (purity > 90%, Jing Zhu bio tech ltd, Nanjing), RPMI-1640 culture fluid (GIBCO company of the U.S.), hyclone (FBS, Bioengineering Research Institute of the Chinese Academy of Medical Sciences), tetramethyl azo azoles salt (MTT, Sigma company), concanavalin A, Con A (ConA), levamisole hydrochloride, dimethyl sulfoxide (Sigma company).
Animal: SPF level Kunming mouse [credit number: SCXK (new 2010-0001)], 7-8 week age, provided by Xinjiang Experimental Animal Center.
Experimental technique
The preparation of need testing solution: get rhodioside, butyl alcohol, cheese thiophene dimension sterling each 4mg be dissolved in 4ml serum-free 1640 culture medium, be diluted to 1000 μ g/ml by 2 times of constant gradients, 500 μ g/ml, 250 μ g/ml, 125 μ g/ml, 62.5 μ g/ml, 31.25 μ g/ml and 15.625 μ g/ml.
Radix Rhodiolae main component is on the impact of mice T, bone-marrow-derived lymphocyte:
The separation of peripheral blood mixed lymphocytes: asepticly take mouse blood (same desirable in vivo test mouse blood), add in the centrifuge tube containing heparin solution, get mice anticoagulation 1ml, after adding the dilution of equivalent Hank ' s liquid, along tube wall slowly add equivalent lymphocyte separation medium is housed go in Centrifugal Electrostatic pipe, blood is made to be overlapped on layering liquid, with horizontal centrifuge with the centrifugal 20min of 2500r/min, now erythrocyte sedimentation is to orlop, upper strata is blood, middle level is layering liquid, the interface of blood plasma and layering liquid has a white to carry secretly as lymphocyte and mononuclearcell, get lymphocyte and mononuclearcell is placed in centrifuge tube, add the erythrocyte cracked liquid of 5 times of volumes, hatch 10min for 37 DEG C, 2 times are washed with Hank ' s liquid, dilution is 5 × 10
6/ ml.
T/B lymphocyte proliferation assay: every hole 5 × 10
6/ ml inoculates, and every hole 100 μ l, is connected to 96 orifice plates.Medicine is added after cultivating 5h.Medicine grouping same " 2.2.3 ", drug-induced group, every hole inducer LPS or ConA 10 μ l, and every hole supplemented medium 40ul; Variable concentrations medicine group, adds medicine 50 μ l; Matched group, supplies by culture medium; After cultivating 48h, every hole adds 20 μ l MTT, and after 4h, absorbance is measured at 570nm place.
The lymphocytic transformation experiment of T/B: every hole 400 μ l is inoculated in 24 orifice plates.Cell is divided into derivant ConA final concentration 5 μ g/ml (for T lymphocyte) or LPS final concentration 25 μ g/ml (for bone-marrow-derived lymphocyte) group, normal group (solvent control) group, variable concentrations positive drug levamisole hydrochloride group (same to medicine), variable concentrations Radix Rhodiolae composition medicine group to be measured; 72h is hatched altogether for each group with mixed lymphocytes.Operate in the following order: PBS rinsing 3 times; Methanol: glacial acetic acid=3:1, fixes 30min in fixative, and Giemsa liquid dyes 10 minutes, tap water.Cover plate dries rear left-hand thread on microscope slide, microscopy, selects 100 cytometers to calculate di.Testing index: di=somatoblast number/total cell number × 100%, di higher than 50% be defined as significantly urge transformation.
The cytophagic impact of Radix Rhodiolae main component dialogue:
The leukocytic preparation of mouse peripheral blood: asepticly take mouse blood (same desirable in vivo test mouse blood), add in the centrifuge tube containing heparin solution, get mice anticoagulation 1ml, after adding the dilution of equivalent Hank ' s liquid, slowly add along tube wall and lymphocyte separation medium is housed in vitro, blood is made to be overlapped on layering liquid, with horizontal centrifuge with the centrifugal 30min of 2000r/min, now erythrocyte sedimentation is to orlop, upper strata is blood, middle level is layering liquid, the interface of blood plasma and layering liquid has a white to carry secretly as lymphocyte and mononuclearcell, get lymphocyte and mononuclearcell is placed in centrifuge tube, add the erythrocyte cracked liquid of 5 times of volumes, hatch 10min for 37 DEG C, 2 times are washed with Hank ' s liquid, dilution is 2 × 10
6/ ml.
Leukocyte is divided into normally (solvent control) group, variable concentrations positive drug (levamisole hydrochloride) group, variable concentrations Radix Rhodiolae composition medicine group to be measured; Every hole 1 × 10
6individual/ml, 200 μ l are inoculated in 96 orifice plates; Cell attachment cultivates 2-3h, discards culture medium and adds variable concentrations medicine cultivation 24h, supernatant discarded; Add the dimethyl diaminophenazine chloride 200 μ l of 0.2%, cultivate after 1h, PBS washes plate 2 times, and every hole adds cell pyrolysis liquid (acetic acid: dehydrated alcohol=1:1) 200 μ l, 4 DEG C of left undisturbed overnight or 37 DEG C, 30min, 570nm place survey OD value; With OD value for vertical coordinate, compare for abscissa does broken line graph with the drug level of correspondence.
Radix Rhodiolae main component is on the impact of Turnover of Mouse Peritoneal Macrophages phagocytic activity:
Turnover of Mouse Peritoneal Macrophages preparation: mice break neck put to death, the normal saline of lumbar injection 5ml pre-cooling.Draw peritoneal exudate 5ml (ice bath), 1600rpm is centrifugal, 10min, abandons supernatant, and 4 DEG C of normal saline are settled to 5ml.Macrophage counting under blood counting chamber mirror.With normal saline dilution macrophage concentration to 1 × 10
6individual/ml, gets 4ml, and 1600rpm is centrifugal, and 10min abandons supernatant, adds 5ml RPM 1640+10%FBS culture medium resuspended.Macrophage is added culture bottle purification and cultivate 2h, supernatant discarded, the trypsinization with 0.25% or cell scrape scraping cells, and culture medium is resuspended.
Macrophage phagocytic dimethyl diaminophenazine chloride is tested: cell is divided into feminine gender (solvent control) group, variable concentrations Radix Rhodiolae composition medicine group to be measured; Every hole 1 × 10
6individual/ml, 200 μ l are inoculated in 96 orifice plates; Cell attachment cultivates 2-3h, discards culture medium and adds variable concentrations medicine cultivation 24h, supernatant discarded; Add the dimethyl diaminophenazine chloride 200 μ l of 0.9%, cultivate after 1h, PBS washes plate 2 times, adds cell pyrolysis liquid (acetic acid: dehydrated alcohol=1:1) the 200 every hole of μ l, 4 DEG C of left undisturbed overnight or 37 DEG C, and 30min, 570nm wavelength surveys OD value; With OD value for vertical coordinate, compare for abscissa does broken line graph with the drug level of correspondence.
Macrophage NO activity identification: with to train liquid by cell dilution be 1 × 10 containing 10%FBS PRMI 1640
6individual/ml, gets 200 μ l/ holes and is inoculated in 96 well culture plates, put 37 DEG C, 5%CO
2hatch 2 hours in cell culture incubator, abandon supernatant (removal non-adherent cell), wash 3 times with PBS, add again containing 10%FBS, PRMI 1640 trains liquid 160 μ l, after cultivating 24h, first adds LPS (1 μ g/ hole), 4 μ g/ μ l, 2 μ l inducing culture 2h, are then divided into feminine gender (solvent control) group, variable concentrations Radix Rhodiolae composition medicine group to be measured, add medicine 40 μ l to be checked respectively, jiggle 96 orifice plates, then continue to cultivate; Cultivate 48h; Test kit operation is measured: (see green skies NO detection kit) according to NO.
Adopt one factor analysis of variance, check each group of mean difference whether to have statistical significance; Inspection level is P≤0.05.Application SPSS for 19.0 statistical software carries out data statistics and analysis.
Result:
Radix Rhodiolae main component is on the impact of mice T, bone-marrow-derived lymphocyte:
Proliferation experiment result: positive drug levamisole hydrochloride (62.5 μ g/ml-250 μ g/ml) to the proliferation of bone-marrow-derived lymphocyte significantly (p≤0.05), and has certain dosage effect; By comparison, butyl alcohol (125 μ g/ml-250 μ g/ml), rhodioside (250 μ g/ml) and cheese thiophene dimension (250 μ g/ml) all have significant proliferation to bone-marrow-derived lymphocyte, and the proliferation trend of butyl alcohol is higher than positive drug group and other two groups, and act on compared with single induction group significantly (p≤0.05), refer to table 15.
Table 15 rhodiosides etc. are to the proliferation of the bone-marrow-derived lymphocyte that LPS induces
Note: compare with blank group: ##p≤0.01; Compare with LPS group:
*p≤0.05, * * p≤0.01
Cheese thiophene dimension when high concentration (250 μ g/ml) to the lymphocytic proliferation of T higher than positive drug group, and act on compared with single induction group remarkable (p≤0.05), refers to table 16.
The lymphocytic proliferation of T that table 16 rhodioside etc. are induced ConA
Note: compare with blank group: ##p≤0.01; Compare with LPS group:
*p≤0.05, * * p≤0.01
Transformation experiment result: butyl alcohol can significantly improve bone-marrow-derived lymphocyte conversion ratio, after 24h, a large amount of resting B cell transforms to transitional type bone-marrow-derived lymphocyte, butyl alcohol (250 μ g/ml) group reaches conversion ratio to 81%, and is only 57.12% (table 17) with the levamisole of concentration; And rhodioside can significantly improve the conversion ratio of inducer T lymphocyte, when high concentration (250 μ g/ml), be 62.25% to the lymphocytic conversion ratio of T, the levamisole with concentration is 47.34%, cheese thiophene dimension only 39.46%.After its effect 24h, silent oscillation T cell is converted into T lymphoblast (table 18) in a large number.
The bone-marrow-derived lymphocyte conversion ratio that table 17 LPS induces
Note: conversion ratio, more than 50%, has significance
The T lymhocyte transformation rate that table 18 ConA induces
Note: conversion ratio, more than 50%, has significance
The cytophagic impact of Radix Rhodiolae main component dialogue:
Phagocytic activity and the humoral immune function of peripheral blood leucocyte are closely related, and positive drug levamisole hydrochloride significantly can improve the leukocytic phagocytic rate of mouse peripheral blood, and has dosage effect; By comparison, rhodioside group is comparatively close with it to leukocytic short phagocytosis, and cheese thiophene dimension group engulfs effect the most weak (table 19) to leukocytic urging;
Table 19 different pharmaceutical group is to leukocytic phagocytosis
Radix Rhodiolae main component is on the impact of peritoneal macrophage phagocytic activity:
Peritoneal macrophage engulfs dimethyl diaminophenazine chloride experimental result: compared with positive controls, Radix Rhodiolae main component all has obvious facilitation to the phagocytic activity of mouse monokaryon macrophage, wherein the facilitation performance of rhodioside is the most obvious, and it is the most weak that the facilitation that cheese thiophene is tieed up shows; And under 50 μ g/ml concentration, short phagocytosis ability maximum (table 20).
Table 20 different pharmaceutical group is to the effect of macrophage phagocytic dimethyl diaminophenazine chloride
The secretory volume of peritoneal macrophage NO activity identification result: NO is an important indicator of reflection macrophage function, and compared with matched group, LPS urgees the effect of macrophages secrete NO significantly (p≤0.01).Radix Rhodiolae main component to macrophages secrete NO without remarkable effect (table 21);
Table 21 different pharmaceutical group is to the comparison of macrophages secrete NO effect
Note: compare with matched group: * p≤0.05, * * p≤0.01)
Analysis and thinking:
Methods of glycosides has immunomodulating, defying age, neurocyte protection, liver protecting, cardiovascular protection, antioxidation, tumor suppression, two-ways regulation to body and affects the effects such as substance metabolism.Research shows, Radix Notoginseng total arasaponins can promote that ConA and LPS two kinds stimulates the mice T of induction, the multiplication capacity of bone-marrow-derived lymphocyte significantly, illustrates that arasaponin can promote cellular immunization, also can promote humoral immunization to a certain extent; The derivant of Radix Ginseng total saponins obviously can promote the lymphocytic propagation of T, illustrates that two kinds of derivants of Radix Ginseng total saponins can strengthen immunocompetence; Inokopolyose can promote B lymphocyte proliferation, but to the lymphocytic propagation of T without facilitation, shows that Inokopolyose has humoral immune function.Glycoside is also the main chemical compositions of Rhodida plant, and octadecyloxy phenyl compounds wherein such as rhodioside and tyrosol, cheese plug dimension etc. are main active component, and pharmaceutical research proves that it has antioxidation, and the multiple effect such as immunomodulating.But its effect target cell is not appeared in the newspapers.
Immunity be body itself to the identification of non-own material and protection system, be the defensive barrier be jointly made up of humoral immunization and cellular immunization.T/B lymphocyte regulates the cell/humoral immunoresponse(HI) of body respectively, is the main effects cell of adaptive immune system.An other immunocyte mononuclear cell is ingredient indispensable in immunity of organism system equally, and himself and the cytokine profiles secreted thereof participate in multiple immune system effect link.
Conclusion:
Large quantity research shows activity and the secretion capacity of macrophage, and the lymphocytic immunocompetence of T/B is the important inspection target of reaction immune system activity.This research shows, and rhodioside is when high concentration (250 μ g/ml), and can not only promote the lymphocytic conversion ratio of T/B and the rate of increase, the phagocytic activity going back Number of Peripheral Blood Leucocyte and peritoneal macrophage has significant castering action.These results show that rhodioside is very extensive to the immunization of mouse immune system, can participate in panimmunity responsing reaction, but also illustrate that its specificity is poor.Another main component butyl alcohol of Radix Rhodiolae (62.5 μ g/ml-250 μ g/ml) then can the propagation of specific stimulation bone-marrow-derived lymphocyte and conversion (P≤0.05), and action effect is higher than positive drug group, this shows that Radix Rhodiolae butyl alcohol specificly can act on humoral immunoresponse(HI) system.Cheese plug dimension then has significant facilitation (p≤0.05) to silent oscillation T cell to blast transformation.Infer thus, Radix Rhodiolae three kinds of main components have promotion cell and humoral immune function, and different main component acts on different immune target cells.
Claims (2)
1. a purposes for Radix Rhodiolae extract, is characterized in that the purposes of this Radix Rhodiolae extract in the medicine of the untoward reaction caused by preparation opposing Radiotherapy chemotherapy.
2. the purposes of described Radix Rhodiolae extract according to claim 1, the untoward reaction that it is characterized in that caused by described opposing Radiotherapy chemotherapy is the untoward reaction of bone marrow depression untoward reaction or immunological function repression.
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| CN1101847A (en) * | 1993-10-21 | 1995-04-26 | 军事医学科学院放射医学研究所 | Process of preparing medicine for treating anemia and promoting leukoblastosis by rhodiola root extract |
| CN101664478A (en) * | 2008-09-03 | 2010-03-10 | 羊敏 | Natural medicament against bone marrow and bone injuries caused by oncological radiotherapy and chemotherapy and preparation method thereof |
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