CN104490957B - A kind of attenuation synergistic composition to cancer chemotherapy containing pattern wood with gingerol - Google Patents
A kind of attenuation synergistic composition to cancer chemotherapy containing pattern wood with gingerol Download PDFInfo
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Abstract
The invention discloses a kind of containing pattern wood and the attenuation synergistic composition to cancer chemotherapy of gingerol, belong to technical field of pharmaceuticals, including pattern wood extract and gingerol component, the weight ratio of the pattern wood extract and gingerol component is 10 25：13, traditional Chinese medicinal components Poria cocos, fructus amomi, evodia rutaecarpa, Semen Cuscutae, saline cistanche, the tuber of pinellia, purple perilla, dried orange peel, excrementum pteropi, blackberry lily can also be added.Said composition is using pattern wood extract and gingerol component as core material, it is equipped with other auxiliary elements and is prepared into natural drug composition, residue in gingerol extraction process, which is retained, substitutes part auxiliary element, both auxiliary element can be used as, active ingredient is also used as, can effectively suppress cancer chemotherapy induced Vomiting and the treatment to cancer plays Synergy and attenuation.After adding traditional Chinese medicinal components, there is synergy to pattern wood extract, gingerol, there is preventive and therapeutic effect to the vomiting after cancer chemotherapy, also there is a certain degree of therapeutic action to cancer, tumour can be suppressed.
Description
Technical field
The present invention relates to a kind of natural drug composition, and in particular to a kind of wood containing pattern subtracts with gingerol to cancer chemotherapy
Malicious synergistic composition, the preparation of the natural drug composition and vomits side effect and right for preparing to reduce caused by cancer chemotherapy
Treating cancer plays synergistic effect.
Background technology
Cancer has turned into the major disease of the mankind, though chemotherapy has a certain curative effect, most of patient occurs sternly after chemotherapy
The reactions such as weight Nausea and vomiting, can cause de- water and rock-soil coupling, malnutrition and metabolic acidosis etc., easily cause to suffer from
There is the nutrient imbalance of body and the collapse of patient body occurs in person, has a strong impact on quality of life of patients, or even sometimes have to
Interrupt treatment.The conventional antiemetic such as histamine, acetylcholine, dopamine receptor antagonistic, hormone and cannabis, to vomit duo to chemotherapy
Weak curative effect, toxic side effect is strong, using being above very limited.The new antiemetic such as NK1 receptor antagonists and 5-HT3 receptor antagonist classes
Medicine, antiemetic effect is good, but expensive, and use range is narrow, therefore the urgently development of natural less toxic anticancer synergia medicine.
Pattern wood (Pistacia chinensis Bunge) is also known as Pistacia chinensis, has the functions such as clearing heat and promoting fluid, anti-inflammatory,
Available for treating gastrointestinal disease.Ginger is described as " vomiting panacea " with its distinctive " gingerol ", can resist copper sulphate and suitable
Vomit caused by platinum, and the release cause of enteron aisle chromaffin cell can be suppressed and tell factor 5-HT3 and Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, reach treatment abdominal distension, diarrhoea and
The effect of vomiting.The present inventor is tested by substantial amounts of formula rate and network pharmacology research, obtains pattern wood and gingerol
The optimal drug comparative compositions of component, it was demonstrated that it has the effect of fine, and energy to the side effect such as vomiting caused by cancer chemotherapy
Play a part of auxiliary and suppress tumour.
The content of the invention
The shortcomings that it is an object of the invention to overcome prior art to exist, disclose containing pattern wood with gingerol to cancer chemotherapy
Attenuation synergistic composition, said composition is equipped with other auxiliary elements using pattern wood extract and gingerol component as core material
It is prepared into natural drug composition, the residue in gingerol extraction process, which is retained, substitutes part auxiliary element, can both be used as auxiliary
Co-ingredients, active ingredient is also used as, and traditional Chinese medicinal components can also be added, can effectively suppress cancer chemotherapy induced Vomiting
And the treatment to cancer plays Synergy and attenuation.
The technical scheme is that：
A kind of attenuation synergistic composition to cancer chemotherapy containing pattern wood with gingerol, it is characterized in that, including pattern wood extract
With gingerol component,
The pattern wood extract and the weight ratio of gingerol component are 10-25：1-3.
Preferably, in addition to traditional Chinese medicinal components,
The traditional Chinese medicinal components are Poria cocos, fructus amomi, evodia rutaecarpa, Semen Cuscutae, saline cistanche, the tuber of pinellia, purple perilla, dried orange peel, excrementum pteropi, are penetrated
It is dry.
Preferably, the Poria cocos, fructus amomi, evodia rutaecarpa, Semen Cuscutae, saline cistanche, the tuber of pinellia, purple perilla, dried orange peel, excrementum pteropi, blackberry lily
Weight ratio be 1:1:1:1:1:1:1:1:1:1.
Present invention also offers a kind of preparation method containing pattern wood with the attenuation synergistic composition to cancer chemotherapy of gingerol,
It is characterized in that comprise the following steps：
(1) pattern wood method for preparing extractive is：
The fresh branch of pattern wood and leaf are taken, dry, pulverize, sieve, 12h is soaked with 3-10 times of ethanol, filters to take clarified solution, centrifuge
15-40 minutes, supernatant is separated, distillation removes ethanol, then is dried under reduced pressure into dry extract, crushes, sieving.The dregs of a decoction add again after extraction
6 times of distilled water, immersion, decoct, filtering, centrifuge 15-35 minutes, separate supernatant, be dried under reduced pressure into dry extract, crush, sieving,
Powder merging twice is obtained into pattern wood extract；
The preparation method of gingerol is：
Ginger is thinly sliced, dried, powder is broken into high speed Universal pulverizer, crosses 30 mesh sieves, then sieving fine powder is put
Enter in the big port grinding bottles of 5000mL, to soak 65-80h under 6 times of amount acetone normal temperature；It is ultrasonic under 40 DEG C, 40KHz ultrasonic frequency
15-25min is extracted, after centrifugation obtains supernatant, it is standby that ginger powder is made in residue drying；Supernatant is depressurized again and filtered, filtrate
It is placed in Rotary Evaporators and is concentrated in vacuo to obtain ginger extraction mixture, then the mixture be repeatedly concentrated and dried and obtains gingerol
Component is standby；
(3) if the attenuation synergistic composition only includes pattern wood extract and gingerol component, then this step is：
It is 10-25 to weigh weight ratio:1-3 pattern wood extract and gingerol component, then it is 20- to be equipped with percentage by weight
The magnesium stearate of 27% ginger powder, 55-65% starch and 1-3%, it is filled in Capsules, is made each after well mixed
Pure heavy 200mg or 500mg capsule；Or it is made 200mg's containing natural drug composition or 500mg by wet granulation technology
Tablet；The ginger powder is the ginger powder of gained in step (2)；
If the attenuation synergistic composition also includes the traditional Chinese medicinal components described in claim 2, then this step is：
Parts by weight as described in Claims 2 or 3 weigh raw material, first soak the starting material with water weighed 2 hours, boiling 30
Minute, pour out decoction；Add water and boil 30 minutes, pour out decoction；Twice of decoction amalgamation rises, and is evaporated under reduced pressure, and is dried to powder, is
Herb powder；Then it is 10-25 to weigh weight ratio:1-3 pattern wood extract and gingerol component, is mixed with above-mentioned herb powder
Uniformly, then ginger powder, 55-65% starch and 1-3% magnesium stearate that percentage by weight is 20-27% are equipped with, be well mixed
After be filled in Capsules, each pure heavy 200mg or 500mg capsule is made；Or it is made by wet granulation technology containing day
The 200mg or 500mg of right pharmaceutical composition tablet；The ginger powder is the ginger powder of gained in step (2).
Further, the weight ratio of the fresh branch of pattern wood and leaf is 1 in the step (1):1.
Further, centrifugal rotational speed is 3000rpm in step (1).
Present invention also offers a kind of attenuation synergistic composition to cancer chemotherapy containing pattern wood and gingerol to treating cancer
Disease plays the purposes in the medicine of synergistic effect.
Natural drug composition of the present invention having the beneficial effect that in terms of emesis and suppression tumour：
1. attenuation synergistic composition to cancer chemotherapy of the present invention containing pattern wood and gingerol has good emesis to act on,
Complete the preclinical pharmaceutical research in mink nausea model.Observe the natural drug composition causes mink to vomit mould to cis-platinum
The antagonism of type over a period to come.Research shows：Vomiting reaction of the natural drug composition for mink caused by cis-platinum
There is obvious antagonism, it acts on the effect better than independent gingerol or pattern wood extract.
2. attenuation synergistic composition to cancer chemotherapy of the present invention containing pattern wood and gingerol has the function that to suppress tumour, existing
The preclinical pharmaceutical research on mouse tumor model is completed.Employ mouse tumor model, observation natural drug combination
Inhibitory action of the thing to mouse interior tumor.Research shows：The natural drug composition has significantly for mouse interior tumor
Inhibitory action.
3. the present invention has carried out the acute toxicology research of mouse, research shows：The natural drug composition is to feeding and going
It is activity etc. without influence, internal organs and pathological observation also no abnormality seen.Illustrate the natural drug composition toxicity very little.
4. pattern wood extract, gingerol are originally combined by the present inventor, and determine these groups by many experiments
/ consumption proportion, said composition has been played good synergy.By repeatedly experimental studies have found that the present invention
Ratio between natural drug composition each component is in 10-25:Acted on during 1-3 obvious.
5. the natural drug composition pattern wood extract, gingerol component can be obtained by conventional meanses, such group
It is relatively low that compound obtains convenience, cost in itself.
6. the attenuation synergistic composition to cancer chemotherapy containing pattern wood and gingerol of the present invention can also add traditional Chinese medicinal components,
The traditional Chinese medicinal components of addition are also natural drug, and also have synergy, the traditional Chinese medicinal components of addition to pattern wood extract, gingerol
There is preventive and therapeutic effect to the vomiting after cancer chemotherapy, cancer has a certain degree of therapeutic action, can suppress tumour, so as to increase
The effect of strong composition.
Embodiment
The preparation of natural drug composition of the present invention is expanded on further below by embodiment and test example, and it is resisting
Vomiting and the beneficial effect of antitumor middle application.
Embodiment
The first step：The preparation of various extracts
(1) prepared by pattern wood extract
Take the fresh branch of pattern wood and leaf (1:1) it, dry, pulverize, sieve, soak 12h with 3 times of ethanol, filter to take clarified solution, from
The heart (3000rpm) 15 minutes, supernatant is separated, distillation removes ethanol, then is dried under reduced pressure into dry extract, crushes, sieving.After extraction
The dregs of a decoction add 6 times of distilled water again, immersion, decoct, filtering, 3000rpm centrifugation 15min, separate supernatant, are dried under reduced pressure into dry leaching
Cream, crush, sieving, powder merging twice is obtained into pattern wood extract.
The preparation of gingerol：
Ginger is thinly sliced, dried, powder is broken into high speed Universal pulverizer, crosses 30 mesh sieves, then sieving fine powder is put
Enter in the big port grinding bottles of 5000mL, to soak 65h under 6 times of amount acetone normal temperature；Ultrasound carries under 40 DEG C, 40KHz ultrasonic frequency
After taking 15min, centrifugation to obtain supernatant, it is standby that ginger powder is made in residue drying；Supernatant is depressurized again and filtered, filtrate is placed in rotation
Turn to be concentrated in vacuo to obtain ginger extraction mixture in evaporimeter, then the mixture be repeatedly concentrated and dried to obtain gingerol component standby
With.
The preparation of herb powder：
Chinese medicine material is weighed (Poria cocos, fructus amomi, evodia rutaecarpa, Semen Cuscutae, saline cistanche, the tuber of pinellia, purple perilla, dried orange peel, excrementum pteropi, to penetrate
It is dry, match as equal portions), first the starting material with water weighed is soaked 2 hours, boiling 30 minutes, pours out decoction；Add water and boil 30 points
Clock, pour out decoction；Twice of decoction amalgamation rises, and is evaporated under reduced pressure, and is dried to powder, is herb powder.
Second step：The preparation of preparation
Formula 1：
Pattern wood extract 100mg and gingerol 20mg；
The preparation of above-mentioned formula capsule preparations
Weigh respectively above-mentioned steps preparation pattern wood extract 100mg and gingerol 20mg, while weigh 100mg ginger powders,
Starch 275mg, magnesium stearate 5mg, uniformly mixing, fill capsule, and each pure heavy 500mg capsule is made.
The preparation of above-mentioned formula tablet
Pattern the wood extract 100mg and gingerol 20mg of above-mentioned steps preparation are weighed respectively, are equipped with 75mg ginger powders, 200mg
Starch and 100mg lactose, with wet granulation, drying after being well mixed, after whole grain with 5mg magnesium stearate mixed pressuring plates, system
Into every pure heavy 500mg tablet.
Formula 2：
Pattern wood extract 75mg, gingerol 10mg, herb powder 35mg；
Herb powder is Poria cocos, and fructus amomi, evodia rutaecarpa, Semen Cuscutae, saline cistanche, the tuber of pinellia, purple perilla, dried orange peel, excrementum pteropi, blackberry lily are pressed
The powder prepared according to above-mentioned steps.
The preparation of above-mentioned formula capsule preparations
The pattern wood extract 75mg and gingerol 10mg of above-mentioned steps preparation, herb powder 35mg are weighed respectively；Claim simultaneously
75mg ginger powders, starch 300mg, magnesium stearate 5mg are taken, uniformly mixing, fill capsule, each pure heavy 500mg capsule is made.
The preparation of above-mentioned formula tablet
The pattern wood extract 75mg and gingerol 10mg of above-mentioned steps preparation, herb powder 35mg are weighed respectively；It is equipped with
The lactose of 75mg ginger powders, 200mg starch and 100mg, with wet granulation, drying after being well mixed, after whole grain with 5mg stearic acid
Magnesium mixed pressuring plate, every pure heavy 500mg tablet is made.
Drug effect and pharmacological data：
Composition is in following experiment examples：Pattern wood extract, the usage ratio of gingerol are 10-25:1-3.Invention
People is to above-mentioned each Ingredient Amount ratio in 10-25:When between 1-3 a variety of proportionings combination obtain composition carried out with it is upper identical
Experiment, find that the natural drug composition in this amount ranges can obtain good synergy, to cancer chemotherapy
Side effect can play good effect and the treatment to cancer has synergistic effect.
If in addition, addition traditional Chinese medicinal components, the traditional Chinese medicinal components of addition are also natural drug, and also to pattern wood extract, ginger
Capsaicin has synergy, and to having preventive and therapeutic effect to the vomiting after cancer chemotherapy, also has a certain degree of control to cancer
Treatment acts on, and can suppress tumour.
Test example 1：The pharmacodynamic study of natural drug composition treatment vomiting
(1) experiment material：Cis-platinum (20mg/ branch), ondansetron hydrochloride parenteral solution (2ml：4mg), healthy adult mink is (male
Property, body weight 1.3-1.8kg), every mink is individually placed in 75cm × 50cm × 50cm iron cage, 24 hours natural light irradiations,
Ad lib is drunk water before experiment；
(2) experimental method：Mink is randomly divided into 5 groups, every group 6, A groups are cis-platinum model control group, and B groups are Ang Dansi
Fine jade positive drug group, C groups are gingerol group, and D groups are pattern wood extract, and E groups are natural drug composition of the invention.A groups
Give physiological saline pretreatment；B groups give Ondansetron 7.5mgkg-1 pretreatments；C groups give gingerol 10gkg-1 (phases
When in containing crude drug amount) ig pretreatments；D groups give pattern wood extract 10gkg-1 (equivalent to containing crude drug amount) ig pretreatments；E groups
Give natural drug composition 10gkg-1 (equivalent to containing crude drug amount) ig pretreatments of the present invention；Each group is in administration 30min
After give cis-platinum 7.5mgkg-1ip.To observe the mink vomiting reaction in 6h upon administration, record its and vomit incubation period, do
Vomit and vomiting number.
(3) experimental result：Compared with control group, each treatment group can substantially mitigate retch and vomiting number and extend vomiting
Incubation period (P<0.01).Pattern wood extract group, gingerol group, natural drug composition of the present invention can significantly reduce cause and vomit rate.
The natural drug composition group of the present invention causes rate of vomitting to be less than Ondansetron group (table 1).
Table 1 is formulated 1 pair of cis-platinum (7.5mgkg-1, ip) and caused to vomit the influence of effect
Note：A is equivalent to containing crude drug amount, the bP compared with control group<0.05, cP<0.01
Test example 2：The pharmacodynamic study of pharmaceutical composition antitumor action of the present invention
Experiment material：Kunming mouse, 20 ± 2g of body weight, provided by medicine inspecting institute of Qingdao City Experimental Animal Center.Before experiment
Ad lib is drunk water, 24h natural light irradiations.Endoxan, the permanent auspicious medical Pharmacy stock Co., Ltd's product in Jiangsu.
Experimental method：
Suppression experiment to S 180 Growth on Mice：Using passage 5-7 days, well-grown milky ascites, with suitable
Amount sterile saline is diluted to tumor cell suspension, cell number 107/mL, every group 12, per the right armpit subcutaneous vaccination of mouse
0.2mL (contains oncocyte 2 × 106).Inoculation next day be grouped at random, experimental group by the present invention pharmaceutical composition be divided into (200,
100,50mgmL-1) three dosage groups, per mouse gavage 0.5ml；Negative control group gives 0.5mL distilled water；Positive drug group
Give endoxan 20gkg-1d-1, successive administration 10 days.Conventinal breeding, drinking-water, food are unlimited.It is discontinued next day, mouse
All de- cervical vertebra is put to death, and is stripped subcutaneous solid tumors tumor mass, is weighed, and tumor control rate (%) is calculated by following equation：Tumor suppression
Rate=(the average knurl weight (T) of average knurl weight (the C)-experimental group of control group) average knurl weight (C) of/control group.
Suppression to rat liver cancer H22 growths is tested：Packet, dosage, administering mode, experimental period and S180 sarcoma strain phases
Together.
Suppression experiment to mouse ehrlich ascites tumor (solid tumor EAC)：Packet, dosage, administering mode, experimental period with
S180 sarcoma strains are identical.
(3) experimental result：Three dosage groups of pharmaceutical composition of the present invention to S180, H22, EAC compared with negative control group,
There are different degrees of inhibitory action, the inhibiting rate highest (table 2,3,4) especially with high dose group to tumour.
Table 2 is formulated 1 capsule to S180The tumor inhibition effect of mouse
Note：Compared with control group,bP<0.05,cP<0.01
Table 3 is formulated 1 capsule to H22The tumor inhibition effect of mouse
Note：Compared with control group,bP<0.05,cP<0.01
Table 4 is formulated tumor inhibition effect of 1 capsule to EAC mouse
Note：Compared with control group,bP<0.05,cP<0.01
Test example 3：The acute toxicity test of natural drug composition of the present invention
(1) experimental animal：Kunming mouse, 20 ± 2g of body weight, provided by medicine inspecting institute of Qingdao City Experimental Animal Center
(2) medicine：Natural drug composition, the tragacanth of the present invention
(3) test method：According to promulgate in National Drug Administration one on November 12, in 999 revision " in
Medicine new drug research technical requirements ", acute toxicity testing is carried out to the natural drug of the present invention.Because being limited by drug concentration and volume
System, LD50 can not be determined, therefore carry out maximum dosage-feeding experiment.Kunming mouse 30 is taken, male and female half and half, ig is given in capsule
Medicine 1gmL-1, every 0.8mL, i.e. mouse maximum gavage volume, with observation post administration mouse active state, diet, excrement,
Breathing, body weight and death condition, Continuous Observation 14d.
(4) result of the test：Well-grown after the administration of each group mouse stomach, increased weight, behavior is normal, be showed no it is dead and
Significant reaction.After animal is put to death, each main organs phenomenon without exception is visually observed.
As a result show, natural drug composition maximum dose oral application of the invention, to animal without obvious infringement, do not send out
Toxic reaction now is produced to body, is a kind of safe and reliable medicine.
For formula 2, test with formula 1, the Contrast on effect data such as table 5 below of formula 1,2.
Table 5 is formulated 1,2,3 effect data
| Group | Tumor control rate (middle dosage) | Retch number | Vomiting number |
| Formula 1 | 49.70%/48.25%/49.06% | 6.83±3.71c | 2.33±2.81 |
| Formula 2 | 61.21%/62.34%/61.08% | 4.52±3.02 | 1.87±2.24 |
Claims (5)
1. a kind of containing pattern wood and the attenuation synergistic composition to cancer chemotherapy of gingerol, it is characterized in that, prepared by following methods and
Into：
（1）Pattern wood method for preparing extractive is：
The fresh branch of pattern wood and leaf are taken, dry, pulverize, sieve, 12h is soaked with 3-10 times of ethanol, filters to take clarified solution, centrifuge 15-
40 minutes, supernatant is separated, distillation removes ethanol, then is dried under reduced pressure into dry extract, crushes, sieving；The dregs of a decoction add 6 times again after extraction
Distilled water, immersion, decoct, filtering, centrifuge 15-35 minutes, separate supernatant, be dried under reduced pressure into dry extract, crush, sieve, will
Powder, which merges, twice obtains pattern wood extract；
（2）The preparation method of gingerol is：
Ginger is thinly sliced, dried, powder is broken into high speed Universal pulverizer, crosses 30 mesh sieves, then sieving fine powder is put into
In the big port grinding bottles of 5000mL, to soak 65-80h under 6 times of amount acetone normal temperature；It is ultrasonic under 40 DEG C, 40KHz ultrasonic frequency
15-25min is extracted, after centrifugation obtains supernatant, it is standby that ginger powder is made in residue drying；Supernatant is depressurized again and filtered, filtrate
It is placed in Rotary Evaporators and is concentrated in vacuo to obtain ginger extraction mixture, then the mixture be repeatedly concentrated and dried and obtains gingerol
Component is standby；
（3）It is 10-25 to weigh weight ratio:1-3 pattern wood extract and gingerol component, then it is 20-27% to be equipped with percentage by weight
Ginger powder, 55-65% starch and 1-3% magnesium stearate, be filled in Capsules, be made each pure heavy after well mixed
200mg or 500mg capsule；Or the piece of 200mg or 500mg containing natural drug composition are made by wet granulation technology
Agent；The ginger powder is step（2）The ginger powder of middle gained.
2. a kind of attenuation synergistic composition to cancer chemotherapy containing pattern wood with gingerol, it is characterized in that, it is peppery by pattern wood extract, ginger
Plain component, traditional Chinese medicinal components and ginger powder composition；The traditional Chinese medicinal components are Poria cocos, fructus amomi, evodia rutaecarpa, Semen Cuscutae, saline cistanche, the tuber of pinellia,
Purple perilla, dried orange peel, excrementum pteropi, blackberry lily；The Poria cocos, fructus amomi, evodia rutaecarpa, Semen Cuscutae, saline cistanche, the tuber of pinellia, purple perilla, dried orange peel, five spirits
Fat, the weight ratio of blackberry lily are 1:1:1:1:1:1:1:1:1:1；
The attenuation synergistic composition is prepared by following methods：
（1）Pattern wood method for preparing extractive is：
The fresh branch of pattern wood and leaf are taken, dry, pulverize, sieve, 12h is soaked with 3-10 times of ethanol, filters to take clarified solution, centrifuge 15-
40 minutes, supernatant is separated, distillation removes ethanol, then is dried under reduced pressure into dry extract, crushes, sieving；The dregs of a decoction add 6 times again after extraction
Distilled water, immersion, decoct, filtering, centrifuge 15-35 minutes, separate supernatant, be dried under reduced pressure into dry extract, crush, sieve, will
Powder, which merges, twice obtains pattern wood extract；
（2）The preparation method of gingerol is：
Ginger is thinly sliced, dried, powder is broken into high speed Universal pulverizer, crosses 30 mesh sieves, then sieving fine powder is put into
In the big port grinding bottles of 5000mL, to soak 65-80h under 6 times of amount acetone normal temperature；It is ultrasonic under 40 DEG C, 40KHz ultrasonic frequency
15-25min is extracted, after centrifugation obtains supernatant, it is standby that ginger powder is made in residue drying；Supernatant is depressurized again and filtered, filtrate
It is placed in Rotary Evaporators and is concentrated in vacuo to obtain ginger extraction mixture, then the mixture be repeatedly concentrated and dried and obtains gingerol
Component is standby；
（3）Traditional Chinese medicinal components are weighed by above-mentioned weight ratio, first the traditional Chinese medicinal components weighed are soaked in water 2 hours, boiling 30 minutes, are fallen
Go out decoction；Add water and boil 30 minutes, pour out decoction；Twice of decoction amalgamation rises, and is evaporated under reduced pressure, and is dried to powder, is traditional Chinese medicine powder
End；Then it is 10-25 to weigh weight ratio:1-3 pattern wood extract and gingerol component, is well mixed with above-mentioned herb powder,
Ginger powder, 55-65% starch and the 1-3% magnesium stearate that percentage by weight is 20-27% are equipped with again, are filled in after well mixed
In Capsules, each pure heavy 200mg or 500mg capsule is made；Or group containing natural drug is made by wet granulation technology
The 200mg or 500mg of compound tablet；The ginger powder is step（2）The ginger powder of middle gained.
3. the preparation method according to claim 1 containing pattern wood with the attenuation synergistic composition to cancer chemotherapy of gingerol,
It is characterized in that comprise the following steps：
（1）Pattern wood method for preparing extractive is：
The fresh branch of pattern wood and leaf are taken, dry, pulverize, sieve, 12h is soaked with 3-10 times of ethanol, filters to take clarified solution, centrifuge 15-
40 minutes, supernatant is separated, distillation removes ethanol, then is dried under reduced pressure into dry extract, crushes, sieving；The dregs of a decoction add 6 times again after extraction
Distilled water, immersion, decoct, filtering, centrifuge 15-35 minutes, separate supernatant, be dried under reduced pressure into dry extract, crush, sieve, will
Powder, which merges, twice obtains pattern wood extract；
（2）The preparation method of gingerol is：
Ginger is thinly sliced, dried, powder is broken into high speed Universal pulverizer, crosses 30 mesh sieves, then sieving fine powder is put into
In the big port grinding bottles of 5000mL, to soak 65-80h under 6 times of amount acetone normal temperature；It is ultrasonic under 40 DEG C, 40KHz ultrasonic frequency
15-25min is extracted, after centrifugation obtains supernatant, it is standby that ginger powder is made in residue drying；Supernatant is depressurized again and filtered, filtrate
It is placed in Rotary Evaporators and is concentrated in vacuo to obtain ginger extraction mixture, then the mixture be repeatedly concentrated and dried and obtains gingerol
Component is standby；
（3）It is 10-25 to weigh weight ratio:1-3 pattern wood extract and gingerol component, then it is 20-27% to be equipped with percentage by weight
Ginger powder, 55-65% starch and 1-3% magnesium stearate, be filled in Capsules, be made each pure heavy after well mixed
200mg or 500mg capsule；Or the piece of 200mg or 500mg containing natural drug composition are made by wet granulation technology
Agent；The ginger powder is step（2）The ginger powder of middle gained.
4. the preparation method according to claim 2 containing pattern wood with the attenuation synergistic composition to cancer chemotherapy of gingerol,
It is characterized in that comprise the following steps：
（1）Pattern wood method for preparing extractive is：
The fresh branch of pattern wood and leaf are taken, dry, pulverize, sieve, 12h is soaked with 3-10 times of ethanol, filters to take clarified solution, centrifuge 15-
40 minutes, supernatant is separated, distillation removes ethanol, then is dried under reduced pressure into dry extract, crushes, sieving；The dregs of a decoction add 6 times again after extraction
Distilled water, immersion, decoct, filtering, centrifuge 15-35 minutes, separate supernatant, be dried under reduced pressure into dry extract, crush, sieve, will
Powder, which merges, twice obtains pattern wood extract；
（2）The preparation method of gingerol is：
Ginger is thinly sliced, dried, powder is broken into high speed Universal pulverizer, crosses 30 mesh sieves, then sieving fine powder is put into
In the big port grinding bottles of 5000mL, to soak 65-80h under 6 times of amount acetone normal temperature；It is ultrasonic under 40 DEG C, 40KHz ultrasonic frequency
15-25min is extracted, after centrifugation obtains supernatant, it is standby that ginger powder is made in residue drying；Supernatant is depressurized again and filtered, filtrate
It is placed in Rotary Evaporators and is concentrated in vacuo to obtain ginger extraction mixture, then the mixture be repeatedly concentrated and dried and obtains gingerol
Component is standby；
（3）Weight ratio weighs traditional Chinese medicinal components as described in claim 2, and first the traditional Chinese medicinal components weighed are soaked in water 2 hours, boiling
30 minutes, pour out decoction；Add water and boil 30 minutes, pour out decoction；Twice of decoction amalgamation rises, and is evaporated under reduced pressure, and is dried to powder,
For herb powder；Then it is 10-25 to weigh weight ratio:1-3 pattern wood extract and gingerol component, is mixed with above-mentioned herb powder
Close uniformly, then be equipped with ginger powder, 55-65% starch and 1-3% magnesium stearate that percentage by weight is 20-27%, after being well mixed
It is filled in Capsules, each pure heavy 200mg or 500mg capsule is made；Or it is made by wet granulation technology containing natural
The 200mg or 500mg of pharmaceutical composition tablet；The ginger powder is step（2）The ginger powder of middle gained.
5. the attenuation synergistic composition to cancer chemotherapy containing pattern wood and gingerol described in claim 1 or 2 is being prepared to treatment
Cancer plays the purposes in the medicine of synergistic effect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410816922.XA CN104490957B (en) | 2014-12-24 | 2014-12-24 | A kind of attenuation synergistic composition to cancer chemotherapy containing pattern wood with gingerol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410816922.XA CN104490957B (en) | 2014-12-24 | 2014-12-24 | A kind of attenuation synergistic composition to cancer chemotherapy containing pattern wood with gingerol |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104490957A CN104490957A (en) | 2015-04-08 |
| CN104490957B true CN104490957B (en) | 2017-12-08 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091313A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Composition of perilla leaf and ginger, preparation method thereof and application thereof in preparing attenuating synergistic medicament in radiotherapy and chemotherapy of cancer |
| CN103908481A (en) * | 2012-12-30 | 2014-07-09 | 刘明 | Use of pistacia chinensis bunge and lance asiabell root extracts in toxicity reduction and efficacy enhancement of cancer chemotherapy |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091313A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Composition of perilla leaf and ginger, preparation method thereof and application thereof in preparing attenuating synergistic medicament in radiotherapy and chemotherapy of cancer |
| CN103908481A (en) * | 2012-12-30 | 2014-07-09 | 刘明 | Use of pistacia chinensis bunge and lance asiabell root extracts in toxicity reduction and efficacy enhancement of cancer chemotherapy |
Non-Patent Citations (2)
| Title |
|---|
| 中药与化疗结合治疗癌症的进展;贺用和;《第五次全国中西医结合中青年学术研讨会论文汇编》;20040731;第127页第3段 * |
| 姜辣素在2种呕吐动物模型中止呕作用机制的探讨;王耀霞等;《沈阳药科大学学报》;20090228;第26卷(第2期);第134-137、164页 * |
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