CN104489162A - Composition, tea paste and application of composition - Google Patents
Composition, tea paste and application of composition Download PDFInfo
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- CN104489162A CN104489162A CN201410796725.6A CN201410796725A CN104489162A CN 104489162 A CN104489162 A CN 104489162A CN 201410796725 A CN201410796725 A CN 201410796725A CN 104489162 A CN104489162 A CN 104489162A
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Abstract
The invention relates to a composition which comprises a pu'er tea extract and an acanthopanax sessiliflorum seem extract. The composition disclosed by the invention has the advantages of safety, stability and no toxic side effect; in addition, the prepared composition has multiple effects including good taste, capability of enhancing immunity, fatigue resistance and stress resistance.
Description
Technical field
The present invention relates to the composition of an extract, more specifically for a kind of, there is antifatigue, the composition and method of making the same improving immunity and anti allergic reaction and purposes.
Background technology
In modern society, work, life stress raise, and people easily produce fatigue, hypoimmunity and stress reaction, and oral, non-toxic and tea product with health role are fatigue-relieving, the best approach improving immunity and anti allergic reaction.
Pu'er tea original producton location is mainly in Simao Diqu and the Xishuangbanna in Yunnan.The many historical book of ancient Chinese has much about the record of Pu'er tea effect: Zhao Xuemin supplementary Amplifications of the Compendium of Materia Medica is recorded, and Pu Hei is as paint, and sober up the first, eliminate indigestion and phlegm, clearing stomach is promoted the production of body fluid, and power is outstanding greatly also; Cloud again in " woody part ", Pu'er tea paste can control all kinds of diseases and ailments, as tripe catches cold, disperses with ginger decoction, and perspiration can be healed, and it is dry that mouth breaks larynx, pain of being heated, and chews namely heal night of making a slip of the tongue with five points; Chen Zonghai writes " interview of the Room, Simao " records " Pu'er is helped digest and dispersed cold, has detoxication "; Clear scholar's Song hero " occupying diet spectrum with breath " cloud " Pu'er product person, highly seasoned kind wind phlegm of telling disappears meat, the dirty string-shaped mass in the abdomen stomachache of all heat, and the diseases such as cholera dysentery are from the beginning of, often healing of drink ".By forefathers to Pu'er tea health-care experience, we are known: Pu'er tea has and helps digestion except poison, and regulate the flow of vital energy swollen, clearing heat and eliminating phlegm, wind dispelling is sobered up, and controls the effects such as dysentery is antibacterial.But the raising of Pu'er tea to immunity, antifatigue is on the weak side.
Summary of the invention
In order to solve problems of the prior art, the invention provides the preparation method of a kind of composition and said composition.Said composition has antifatigue, improves the effect of immunity and anti allergic reaction.
Main purpose of the present invention is to provide a kind of composition, and described composition contains Pu'er tea and Sessileflower Acanthopanax Bark extract composition.
Preferably, described composition is made up of 3-9 weight portion Pu'er tea and 1-4 weight portion Sessileflower Acanthopanax Bark extract.
Preferably, described composition is made up of 3 weight portion Pu'er teas and 1 weight portion Sessileflower Acanthopanax Bark extract.
Preferably, described composition is made up of 4 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts.
Preferably, described composition is made up of 9 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts.
As one embodiment of the present invention, above-mentioned composition can also comprise licorice.
Preferably, described composition is made up of 3 weight portion Pu'er teas, 1 weight portion Sessileflower Acanthopanax Bark extract and 0.1 weight portion licorice.
Term " Pu'er tea " is for raw material with solar dried green tea in the Yunnan large-leaf seed in Yunnan Province's certain area, the loose tea processed through after fermentation and compressed tea, now make a general reference the tea that Pu'er tea district produces, with solar dried green tea in the Yunnan large-leaf seed in generally acknowledged Pu'er tea district for raw material, the loose tea of being processed into through after fermentation and compressed tea; Appearance color is brown red, and endoplasm soup look red is dense bright, the unique Chen Xiang of fragrance, and mellow time of flavour is sweet, brown red at the bottom of leaf; Have raw Pu'er tea and ripe Pu'er tea point, the former is formed by spontaneous fermentation, and the latter is formed by artificial repening.
Term " Sessileflower Acanthopanax Bark " (Acanthopanax sessiliforus) has another name called crow, cortex acanthopanacis wood, Panax sessiliflorus, Araliaceae Acanthopanax machaka or dungarunga plant, high 2-5 rice.Bark lead.Stingless or scattered sturdy straight thorn.Its stem, leaf, fruit are proved edible.Described Sessileflower Acanthopanax Bark extract of the present invention includes Sessileflower Acanthopanax Bark leaf extract, Sessileflower Acanthopanax Bark rhizome extract or Sessileflower Acanthopanax Bark berry extract.
Term " licorice " refers to the solid extract obtained through super-dry with liquid extract of liquorice.
Term " Pu'er tea " and " Sessileflower Acanthopanax Bark extract " refer to and from described Pu'er tea and/or Sessileflower Acanthopanax Bark, extract the material obtained according to ordinary skill in the art means.The described extract extraction step that may comprise because of necessity is mixed into, and the material that cannot or hardly remove economically, but other Chinese medicines do not comprised except Pu'er tea and Sessileflower Acanthopanax Bark and extract thereof.
Another object of the present invention is the preparation method providing described composition, and described preparation method comprises:
1) get Pu'er tea by water extraction, reduced pressure concentration becomes condensed cream, and drying is prepared into Pu'er tea;
2) extract Sessileflower Acanthopanax Bark with polar solvent, reduced pressure concentration becomes condensed cream, is dried to Sessileflower Acanthopanax Bark extract;
3) described Pu'er tea and described Sessileflower Acanthopanax Bark extract is proportionally mixed.
Preferably, described step 1) in the preparation method of Pu'er tea comprise:
Pu'er tea boiling is extracted 2-4 time, each 0.5-2 hour, add the water of 6-12 times of volume, and obtain extract and filter, filtrate is evaporated to tealeaves weight under≤70 DEG C of conditions: concentrate volume=1: 2-1: 3; Concentrate centrifuge, it is 1.1-1.25 that centrifugate is evaporated to 45-65 DEG C of proportion, condensed cream spraying dry or microwave drying.
Preferably, described step 2) in the preparation method of Sessileflower Acanthopanax Bark extract be:
Sessileflower Acanthopanax Bark, pulverizes, adds the water of 10 times of volumes, at soaking at room temperature 30-60min, boil 30-60min afterwards, filters, and collects filtrate; Add the water boil 20-40min of 10 times of volumes again, collect filtrate, merge the filtrate of twice, reduced pressure concentration becomes paste final vacuum or spraying dry.
In some of the preferred embodiment of the invention, described composition is 3-9 weight portion Pu'er tea and 1-4 weight portion Sessileflower Acanthopanax Bark extract, and such as described Pu'er tea is from 3 to 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5 or 9 weight portions, or from 3.5 to 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5 or 9 weight portions, or from 4 to 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5 or 9 weight portions, or from 4.5 to 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5 or 9 weight portions, or from 5 to 5.5, 6, 6.5, 7, 7.5, 8, 8.5 or 9 weight portions, or from 5.5 to 6, 6.5, 7, 7.5, 8, 8.5 or 9 weight portions, or from 6 to 6.5, 7, 7.5, 8, 8.5 or 9 weight portions, or from 6.5 to 7, 7.5, 8, 8.5 or 9 weight portions, or from 7 to 7.5, 8, 8.5 or 9 weight portions, or from 7.5 to 8, 8.5 or 9 weight portions, or from 8 to 8.5 or 9 weight portions, or from 8.5 to 9 weight portions, described Sessileflower Acanthopanax Bark extract is from 1 to 1.5,2,2.5,3,3.5,4,4.5 or 5 weight portions, from 1.5 to 2,2.5,3,3.5,4,4.5 or 5 weight portions, or from 2 to 2.5,3,3.5,4,4.5 or 5 weight portions, or from 2.5 to 3,3.5,4,4.5 or 5 weight portions, or from 3 to 3.5,4,4.5 or 5 weight portions, or from 3.5 to 4,4.5 or 5 weight portions, or from 4 to 4.5,5 weight portions, or from 4.5 to 5 weight portions.
Preferably, described composition is made up of 3-9 weight portion Pu'er tea and 1-4 weight portion Sessileflower Acanthopanax Bark extract.
Preferably, described composition is made up of 3 weight portion Pu'er teas and 1 weight portion Sessileflower Acanthopanax Bark extract.
Preferably, described composition is made up of 4 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts.
Preferably, described composition is made up of 2 weight portion Pu'er teas and 1 weight portion Sessileflower Acanthopanax Bark extract.
In some of the preferred embodiment of the invention, described aqueous solvent is selected from the mixed solvent system that water, the mixable solvent of water and water form.In a preferred embodiment of the present invention, described aqueous solvent is water.In some of the preferred embodiment of the invention, the mixable solvent of described water includes but are not limited to ethanol.In some of the preferred embodiment of the invention, in described mixed solvent system, the volume of water is 30-99.9%, such as, from 30% to 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 35% to 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 40% to 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 45% to 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 50% to 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 55% to 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 60% to 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 65% to 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 70% to 75%, 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 75% to 80%, 85%, 90%, 95%, 98%, 99% or 99.9%, or from 80% to 85%, 90%, 95%, 98%, 99% or 99.9%, or from 85% to 90%, 95%, 98%, 99% or 99.9%, or from 90% to 95%, 98%, 99% or 99.9%, or from 95% to 98%, 99% or 99.9%, or from 98% to 99% or 99.9%, or from 99% to 99.9%, in described mixed solvent system, the volume of water is preferably 50-95%, is more preferably 75-90%.
Another object of the present invention is to provide a kind of tea cream, and the active ingredient of described tea cream is made up of Pu'er tea and Sessileflower Acanthopanax Bark extract.
Preferably, described tea cream is made up of 3-9 weight portion Pu'er tea and 1-4 weight portion Sessileflower Acanthopanax Bark extract.
Preferably, described composition is made up of 3 weight portion Pu'er teas and 1 weight portion Sessileflower Acanthopanax Bark extract.
Preferably, described composition is made up of 4 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts.
Preferably, described composition is made up of 9 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts.
Another object of the present invention is to provide a kind of tea beverage, and described tea beverage contains described composition.
As one embodiment of the present invention, the described water-soluble solution of tea beverage 0.25g above-mentioned composition 250ml adds carrier well known to those skilled in the art composition again.
Another object of the present invention is to provide a kind of pharmaceutical composition, and described pharmaceutical composition contains described composition.
Another object of the present invention is to provide described composition or tea cream and promotes application in the health food of immunity or medicine as preparation.
Another object of the present invention is to provide described composition or tea cream as the application in the health food of preparation antifatigue or medicine.
Another object of the present invention is to provide described composition or tea cream as the application prepared in the health food of resisting stress or medicine.
Preferably, described stress, comprise oxygen deprivation stress, heat stress, cold stress and fatigue stress.
Beneficial effect of the present invention is:
(1) described composition safely, stablize, have no side effect, tea product mouthfeel prepared therefrom is good.
(2) described composition has the multiple action of resisting stress, antifatigue and raising immunity.
(3) when Pu'er tea and Sessileflower Acanthopanax Bark extract resisting stress, there is synergy.
Detailed description of the invention
Term " contains " in the present invention, " comprising ", " comprising ", " primarily of ... composition " can alternatively use, and representing in composition of the present invention or mixture can also containing some composition do not pointed out, namely this term is open; And term " by ... composition " then represents and only represented by pointed composition, but can with impurity, and this impurity only allows to exist with common content, and namely this term is enclosed.
The stem of the Sessileflower Acanthopanax Bark used in the following example, but select Sessileflower Acanthopanax Bark to become limitation of the invention those skilled in the art to know not, also can reach effect of the present invention with Sessileflower Acanthopanax Bark leaf or Sessileflower Acanthopanax Bark fruit; Pu'er tea is purchased from Kunming He Changxuan Tea Co., Ltd; The routine test that other test material is those skilled in the art is selected.
The preparation of embodiment 1 Pu'er tea
Pu'er tea 1000g adds the water boiling and extraction 3 times of 10 times of volumes, and each 0.5 hour, filtered by extract, filtrate is evaporated to tealeaves weight under≤70 DEG C of conditions: concentrate volume is 1: 2, and then spraying dry, obtains Pu'er tea.
The preparation of embodiment 2 Sessileflower Acanthopanax Bark extract
Sessileflower Acanthopanax Bark 1000g, pulverizes, adds the water of 10 times of volumes, at soaking at room temperature 300min, boil 60min afterwards, filters, and collects filtrate; Add the water boil 20-40min of 10 times of volumes again, collect filtrate; Merge the filtrate of twice, after reduced pressure concentration becomes paste≤70 DEG C of vacuum drying, obtain Sessileflower Acanthopanax Bark extract.
The preparation of embodiment 3 composition
Sessileflower Acanthopanax Bark extract 10g prepared by Pu'er tea 30g embodiment 1 prepared and embodiment 2, mixes.
The preparation of embodiment 4 composition
Sessileflower Acanthopanax Bark extract 40g prepared by Pu'er tea 90g embodiment 1 prepared and embodiment 2, mixes.
The preparation of embodiment 5 composition
Sessileflower Acanthopanax Bark extract 40g prepared by Pu'er tea 40g embodiment 1 prepared and embodiment 2, mixes.
The preparation of embodiment 6 composition
Sessileflower Acanthopanax Bark extract 10g prepared by Pu'er tea 20g embodiment 1 prepared and embodiment 2, mixes.
The preparation of embodiment 7 composition
Sessileflower Acanthopanax Bark extract 10g prepared by Pu'er tea 30g embodiment 1 prepared and embodiment 2, licorice 1g mixes.
The acute toxicity test of embodiment 8 composition
Carry out acute toxicity test in mice by maximal tolerance dose method, select SPF level BI/F1 for healthy mice 20 (female, each 10 of hero), quarantine 3d, weight 18.0-21.2g, animal fasting 16h before test, freely drinks water.Given the test agent is that composition sterilized water prepared by embodiment 5 is prepared, and maximum dose mass concentration is 0.2g/ml, and by 3 gavages in maximum gavage capacity 20ml/kgbw 1d, every minor tick 4h, the dosage that gives of given the test agent acute toxicity is 12g/kgbw.Continuous Observation 14d, record poisoning manifestations and death condition.Result of the test is in table 1.
Table 1 its mouse oral acute toxicity testing data
From table 1: during experimental observation, product is on Mice Body quality without impact, and each treated animal diet and activity normally, have no any poisoning symptom, also the phenomena of mortality do not occur.Female, the male mice acute oral MTD of product are greater than 12g/kgbw, belong to non-toxic type.
The taste appraisal test of embodiment 9 composition
Experimental design: Stochastic choice 250 volunteers, wherein man 125, female 125, volunteer's age 10-80 year between, according to sex and age 250 volunteers are divided into A, B, C, D, E, six groups, often organize 50 people.
A group volunteer, tastes the composition of embodiment 3 gained;
B group volunteer, tastes the composition of embodiment 4 gained;
C group volunteer, tastes the composition of embodiment 5 gained;
D group volunteer, tastes the composition of embodiment 6 gained;
E group volunteer, tastes the composition of embodiment 7 gained
Drinking method: the composition 0.25g of gained is all dissolved in 85 DEG C of hot pure water of 200ml by each group, drinks 3 times for each person every day, drink with 3 days according to the temperature be applicable to.
Statistical result showed: A, B, C, D and E group all reflects that the dark brown pool of the composition preparation of each correspondence group is red gorgeous bright, and fragrance Chen Xiang is lasting, mouthfeel alcohol and thick cunning, aftertaste glycol.Wherein E group also has dense sweet taste, and this result shows: the composition obtained by the present invention has good mouthfeel and local flavor.
The anti-fatigue test of embodiment 10 composition
Certain man, 49 years old, Top Management, worked long hours, and the time of having a rest is less, and operating pressure is large, almost not free exercise.Easily occur fatigue and weak in work, decrease of memory, appetite reduces, work efficiency drop.Take composition prepared by embodiment 1, composition 0.25g is dissolved in drink every day in the hot pure water of 250ml 85 DEG C and is no less than 3 times, after one week, under comparable operating conditions, degree of fatigue obviously alleviates, and the dizziness caused because of fatigue disappears substantially, and memory is obviously improved, appetite is obviously recovered, and operating efficiency significantly improves.
Certain man, 47 years old, company middle level, longevity of service, the time of having a rest was less, and the pressure faced in life is large, almost not free exercise.Easily occur fatigue and weak at work, decrease of memory, appetite reduces, and DOMS is dizzy etc.Take composition prepared by embodiment 1, composition 0.25g is dissolved in the hot pure water of 250ml 85 DEG C, drinks every day and be no less than 3 times.After taking two weeks, under comparable operating conditions, degree of fatigue obviously alleviates, and because of the dizzy basic disappearance that fatigue causes, memory is obviously improved, and appetite is obviously recovered, and DOMS obviously improves, and health energy recovers substantially.
The impact test of embodiment 11 present composition immune function of mice
1, testing product: embodiment 3, embodiment 4, embodiment 5, composition prepared by embodiment 6.
2, detect with animal: mouse, rat, male and female half and half.
3, test basis: the Ministry of Public Health " health food inspection and assessment technical specification) " (2003 editions)
4, test item:
4.1 body weight, thymus gland/body weight ratio, spleen/body weight ratio
The mouse spleen lymphocyte transformation experiment (mtt assay) of 4.2 inductions
The mouse Tardive allergy (DTH) of 4.3 dinitrofluorobenzene inductions
4.4 antibody-producting cells detect (Jerne improves slide method)
4.5 serolysin test
4.6 mouse carbonic clearance tests
4.7NK cytoactive detection
5, testing result and judgement
The body weight of 5.1 animals used as test, thymus gland/body weight ratio, and the testing result of spleen/body weight ratio shows, under this experimental condition, institute's full pattern product 500,1000,3000mg/kg (be equivalent to respectively be grown up per kg body weight per day recommended intake 5 times, 10 times, 30 times) dosage time, four groups of samples are to the body weight of animal, and thymus gland/body weight ratio, spleen/body weight ratio is showed no obvious impact.
Mouse spleen lymphocyte transformation experiment (mtt assay) result of 5.2 inductions shows: under this experimental condition, each dosage group optical density difference and solvent control class value, through comparing without statistical significant difference (P > 0.05), show that sample is without enhancing mouse lymphocyte multiplication capacity.
The result of the test of the mouse Tardive allergy (DTH) of 5.3 dinitrofluorobenzene inductions shows, under this experimental condition, each dosage group optical density difference and solvent control class value are through comparing all without statistical significant difference (P > 0.05), and prompting sample has the mouse Tardive allergy effect strengthening dinitrofluorobenzene and cause.Test dose group is positive, and prompting sample can strengthen mouse cell immunologic function.
The result of the test that 5.4 antibody-producting cells detect (Jerne improves slide method) shows, under this experimental condition, each dosage group hemolysis plaque number all to compare through statistics higher than solvent control class value and has significant difference (P < 0.05), and prompting sample has and strengthens the ability that mouse produces antibody producing cells.
5.5 serolysin test results show, under this experiment condition, each dosage group mice serum HD50 value compares with solvent control class value, and all without statistically-significant difference (P > 0.05), prompting sample produces the ability of serum hemolysin without strengthening mouse.The dosage group of a test is positive, and prompting sample can strengthen mouse humoral immune function.
The result of the test of 5.6 mouse carbonic clearance shows, under this experimental condition, each dosage group mouse engulf tree planting A with solvent control class value through comparing all without statistical significant difference (P > 0.05), prompting sample is without the effect strengthening carbonic clearance ability.
The measurement result of 5.7NK cytoactive shows, under this experimental condition, the NK cytoactive of each dosage group mouse is all higher than solvent control class value, and high dose group has statistical significant difference (P < 0.05), prompting sample has the effect of the NK cytoactive strengthening mouse.
Comprehensive above result of the test, according to health food develop immunitypty functional evaluation standard, the composition prepared by the present invention has the function of develop immunitypty.
The resisting stress test of embodiment 12 composition
1, the resist oxygen lack of mouse is tested
Get 70 mouse (Kunming mouse, body weight 18-22g, male and female half and half) to be divided at random 7 groups (see table 2).Wherein the 1st group is that the Pu'er tea embodiment 1 prepared is in the solution being made into 10% (w/v) concentration before use, 2nd group is that the Sessileflower Acanthopanax Bark extract embodiment 2 prepared is in the solution being made into 10% (w/v) concentration before use, 3-6 group be the composition respectively prepared by embodiment 3-6 in the solution being made into 10% (w/v) concentration before use, the 7th group is blank group.Each group gives relative medicine respectively, all by 0.2ml/10g dosage, every day gastric infusion 2 times, successive administration 7d.After last administration 30min, mouse is put into the ground wide-mouth bottle (soda lime padding filter paper in order to absorb urine) filling 10g soda lime, 1 every bottle, bottleneck is coated with vaseline, airtight container, tightens bottle stopper, to breathe as index for the last time, the record mouse time-to-live under anoxic conditions, the results are shown in Table 2.
The hypoxia endurance test result of table 2 mouse
Result of the test shows: each test group relative to the mouse hypoxia endurance test result difference with physiological saline group extremely significantly (P < 0.001), shows that extract prepared by embodiment 1-6 or composition have the effect of resist oxygen lack; 2nd, difference remarkable (P > 0.05) between 3,4,5,6 groups, but 2,3,4,5,6 groups of significant differences (P < 0.05) relative to the 1st group, prove that Sessileflower Acanthopanax Bark extract has stronger anti-mouse anoxia functions, and although the consumption of the composition Sessileflower Acanthopanax Bark that Sessileflower Acanthopanax Bark extract and Pu'er tea form reduces but the mouse survival time is longer, when proving two kinds of extracts associatings, there is synergy.
2, the high temperature resistant experiment of mouse
Get 70 mouse (Kunming mouse, body weight 18-22g, male and female half and half) to be divided at random 7 groups (see table 3).Wherein, 1st group is that the Pu'er tea embodiment 1 prepared is in the solution being made into 10% (w/v) concentration before use, 2nd group is that the Sessileflower Acanthopanax Bark extract embodiment 2 prepared is in the solution being made into 10% (w/v) concentration before use, 3-6 group be the composition respectively prepared by embodiment 3-6 in the solution being made into 10% (w/v) concentration before use, the 7th group is blank group.Each group gives relative medicine respectively, all by 0.2ml/10g dosage, every day gastric infusion 2 times, mouse is put into 250ml conical flask by successive administration 7d, then conical flask is placed in thermostat water bath that temperature is (42 ± 0.5) DEG C, and with gauze, bottleneck is sealed, record mouse time-to-live with this understanding, result of the test table 3.
The high temperature resistance result of the test of table 3 mouse
Result of the test shows: each test group relative to the mouse resistance hot test difference with blank group extremely significantly (P < 0.001), has the effect of high temperature resistance; 2nd, difference remarkable (P > 0.05) between 3,4,5,6 groups, but 2,3,4,5,6 groups relative to the 1st group of significant difference (P < 0.05), prove that Sessileflower Acanthopanax Bark extract has stronger anti-mouse high temperature resistance effect, although and in the composition that Sessileflower Acanthopanax Bark extract and Pu'er tea form the consumption of Sessileflower Acanthopanax Bark reduce but the mouse survival time longer, when proving the associating of two kinds of extracts, there is synergy.
3, the low temperature resistant experiment of mouse
Get 70 mouse (Kunming mouse, body weight 18-22g, male and female half and half), be divided at random 7 groups (see table 4).Wherein, 1st group is that the Pu'er tea embodiment 1 prepared is in the solution being made into 10% (w/v) concentration before use, 2nd group is that the Sessileflower Acanthopanax Bark extract embodiment 2 prepared is in the solution being made into 10% (w/v) concentration before use, 3-6 group be the composition respectively prepared by embodiment 3-6 in the solution being made into 10% (w/v) concentration before use, the 7th group is blank group.Each group gives relative medicine respectively, and all by 0.2ml/10g dosage, every day, gastric infusion 2 times, was placed in the low temperature refrigerator of-25 DEG C by mouse, the record mouse survival time, the results are shown in Table 4.
The low temperature resistant test result of table 4 mouse
Result of the test shows: each test group relative to the mouse result difference of resistance to low temperature resistant test with blank group extremely significantly (P < 0.001), has low temperature resistant effect; 2nd, difference remarkable (P > 0.05) between 3,4,5,6 groups, but 2nd, 3,4,5,6 groups relative to the 1st group of significant difference (P < 0.05), prove that Sessileflower Acanthopanax Bark extract has the cryophylactic effect of stronger anti-mouse, although and in the composition that Sessileflower Acanthopanax Bark extract and Pu'er tea form the consumption of Sessileflower Acanthopanax Bark reduce but the mouse survival time longer, when proving the associating of two kinds of extracts, there is synergy.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included in the meat of protection scope of the present invention.
Claims (10)
1. a composition, is characterized in that, described composition contains Pu'er tea and Sessileflower Acanthopanax Bark extract composition;
Preferably, described composition is made up of Pu'er tea and Sessileflower Acanthopanax Bark extract;
Preferably, described composition is made up of 3-9 weight portion Pu'er tea and 1-4 weight portion Sessileflower Acanthopanax Bark extract;
Preferably, described composition is made up of 3 weight portion Pu'er teas and 1 weight portion Sessileflower Acanthopanax Bark extract;
Preferably, described composition is made up of 4 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts;
Preferably, described composition is made up of 9 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts.
2. prepare a method for composition according to claim 1, it is characterized in that:
1) get Pu'er tea by water extraction, reduced pressure concentration becomes condensed cream, is drying to obtain Pu'er tea;
2) extract Sessileflower Acanthopanax Bark with polar solvent, reduced pressure concentration becomes condensed cream, is drying to obtain Sessileflower Acanthopanax Bark extract;
3) described Pu'er tea and described Sessileflower Acanthopanax Bark extract is proportionally mixed;
Preferably, described step 1) in the preparation method of Pu'er tea comprise:
Pu'er tea boiling is extracted 2-4 time, each 0.5-2 hour, add the water of 6-12 times of volume, obtains extract and filters, filtrate is evaporated to tealeaves weight under≤70 DEG C of conditions: concentrate volume=1: 2-1: 3; By concentrate centrifuge, centrifugate is 1.1-1.25 through being evaporated to 45-65 DEG C of proportion, and condensed cream spraying dry or microwave drying, obtain Pu'er tea.
3. composition according to claim 1, is characterized in that, described Sessileflower Acanthopanax Bark extract is Sessileflower Acanthopanax Bark leaf extract, Sessileflower Acanthopanax Bark rhizome extract or Sessileflower Acanthopanax Bark berry extract.
4. composition according to claim 1, is characterized in that, described Pu'er tea is made a living puerh tea leaves extract or ripe puerh tea leaves extract.
5. composition according to claim 1, is characterized in that, described composition can also comprise licorice;
Preferably, described composition is made up of the licorice of 3 weight portion Pu'er teas, 1 weight portion Sessileflower Acanthopanax Bark extract and 0.1 weight portion.
6. a tea cream, is characterized in that, the active ingredient of described tea cream is made up of Pu'er tea and Sessileflower Acanthopanax Bark extract;
Preferably, described tea cream is made up of 3-9 weight portion Pu'er tea and 1-4 weight portion Sessileflower Acanthopanax Bark extract;
Preferably, described tea cream is made up of 3 weight portion Pu'er teas and 1 weight portion Sessileflower Acanthopanax Bark extract;
Preferably, described tea cream is made up of 4 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts;
Preferably, described tea cream is made up of 9 weight portion Pu'er teas and 4 weight portion Sessileflower Acanthopanax Bark extracts.
7. a tea beverage, is characterized in that, described tea beverage comprises composition according to claim 1.
8. a pharmaceutical composition, is characterized in that, described pharmaceutical composition comprises composition according to claim 1-6 or any one of claim 7 or tea cream.
9. the composition according to claim 1-6 or any one of claim 7 or tea cream are as the application in the health food of preparation antifatigue or medicine.
10. the composition according to claim 1-6 or any one of claim 7 or tea cream are as the application prepared in the health food of resisting stress or medicine.
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| CN201410796725.6A CN104489162A (en) | 2014-12-11 | 2014-12-11 | Composition, tea paste and application of composition |
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| CN201410796725.6A CN104489162A (en) | 2014-12-11 | 2014-12-11 | Composition, tea paste and application of composition |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108124992A (en) * | 2018-01-04 | 2018-06-08 | 中国科学院昆明植物研究所 | A kind of liver protection Pu'er tea and its preparation method and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101107996A (en) * | 2007-08-10 | 2008-01-23 | 游文龙 | Pu'er tea health care beverage and method of manufacturing the same |
| CN101253903A (en) * | 2008-04-07 | 2008-09-03 | 北京惠中铭捷生物科技有限公司 | Health food and its preparation with fat-reducing function |
| CN101536717A (en) * | 2009-04-30 | 2009-09-23 | 陈柏祥 | Health-preserving Pu'er tea |
| CN104187653A (en) * | 2014-08-13 | 2014-12-10 | 张志科 | Composition, tea cream and application |
| CN104186750A (en) * | 2014-07-16 | 2014-12-10 | 吴茹珍 | Eucommia bark herbal health-preserving tea |
-
2014
- 2014-12-11 CN CN201410796725.6A patent/CN104489162A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101107996A (en) * | 2007-08-10 | 2008-01-23 | 游文龙 | Pu'er tea health care beverage and method of manufacturing the same |
| CN101253903A (en) * | 2008-04-07 | 2008-09-03 | 北京惠中铭捷生物科技有限公司 | Health food and its preparation with fat-reducing function |
| CN101536717A (en) * | 2009-04-30 | 2009-09-23 | 陈柏祥 | Health-preserving Pu'er tea |
| CN104186750A (en) * | 2014-07-16 | 2014-12-10 | 吴茹珍 | Eucommia bark herbal health-preserving tea |
| CN104187653A (en) * | 2014-08-13 | 2014-12-10 | 张志科 | Composition, tea cream and application |
Non-Patent Citations (1)
| Title |
|---|
| 冯颖等: "无梗五加果多糖组成及生物活性的研究", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108124992A (en) * | 2018-01-04 | 2018-06-08 | 中国科学院昆明植物研究所 | A kind of liver protection Pu'er tea and its preparation method and application |
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