CN104483473A - Detection reagent for epithelial tissue cancer cells and preparing and detecting method - Google Patents
Detection reagent for epithelial tissue cancer cells and preparing and detecting method Download PDFInfo
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- CN104483473A CN104483473A CN201410854634.3A CN201410854634A CN104483473A CN 104483473 A CN104483473 A CN 104483473A CN 201410854634 A CN201410854634 A CN 201410854634A CN 104483473 A CN104483473 A CN 104483473A
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 67
- 238000001514 detection method Methods 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 12
- 210000000981 epithelium Anatomy 0.000 title abstract description 8
- 206010028980 Neoplasm Diseases 0.000 title abstract description 5
- 201000011510 cancer Diseases 0.000 title abstract 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000000243 solution Substances 0.000 claims abstract description 38
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims abstract description 25
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 20
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229960000907 methylthioninium chloride Drugs 0.000 claims abstract description 14
- 235000019152 folic acid Nutrition 0.000 claims abstract description 13
- 239000011724 folic acid Substances 0.000 claims abstract description 13
- 239000007974 sodium acetate buffer Substances 0.000 claims abstract description 13
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims abstract description 13
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229960000304 folic acid Drugs 0.000 claims abstract description 12
- 229930091371 Fructose Natural products 0.000 claims abstract description 10
- 239000005715 Fructose Substances 0.000 claims abstract description 10
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 10
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 10
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 10
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 10
- 239000004094 surface-active agent Substances 0.000 claims abstract description 9
- 239000008213 purified water Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 229920000742 Cotton Polymers 0.000 claims description 34
- 208000034179 Neoplasms, Glandular and Epithelial Diseases 0.000 claims description 27
- 238000003780 insertion Methods 0.000 claims description 19
- 230000037431 insertion Effects 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 18
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 6
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000005070 sampling Methods 0.000 claims description 6
- 241001411320 Eriogonum inflatum Species 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000001125 extrusion Methods 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 230000003647 oxidation Effects 0.000 abstract description 3
- 238000007254 oxidation reaction Methods 0.000 abstract description 3
- 230000000638 stimulation Effects 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 30
- 210000004027 cell Anatomy 0.000 description 19
- 238000004043 dyeing Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 5
- 210000004881 tumor cell Anatomy 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 238000010827 pathological analysis Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 229920002527 Glycogen Polymers 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 229940064302 folacin Drugs 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
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Abstract
The invention provides a detection reagent for epithelial tissue cancer cells and a preparing and detecting method. The detection reagent for the epithelial tissue cancer cells is high in detecting sensitivity and reliable in detecting result. According to the detecting method, a whole-process sealing pushing-injecting mode is used, stimulation on a patient from special stink from a solution is avoided, meanwhile, time of solution and air contacting is shortened, a false positive result caused by quick oxidation is avoided, and detecting result accuracy is fully guaranteed. The detection reagent for the epithelial tissue cancer cells comprises, by weight, the components of 0.03%-2% of methylene blue, 5%-10% of fructose, 0.1%-1% of ascorbic acid, 1%-5% of folic acid, 40% of dimethyl sulfoxide, 3%-5% of acetic acid-sodium acetate buffer solution, 0.1%-1% of surface active agent and the balance of purified water.
Description
Technical field
The present invention relates to a kind of detection reagent, specifically, be a kind of detection reagent for epithelial neoplasms cell, the invention still further relates to preparation method and the detection method of this detection reagent.
Background technology
At present, the method for carrying out cell specific stain for epithelial tissue mainly contains acetic acid albefaction reaction, iodine test and methylene blue staining etc.Diagnose the illness outside specific stain liquid very long history, is a kind of external Staining for Diagnosis method of intrusive mood, is widely used in the primary dcreening operation of the conventional pathologies such as epithelial tissue.
Acetic acid albefaction reaction is by 3%-5% acetic acid solution, spreads upon on epithelial tissue, and observe the epithelial tissue smeared after waiting for a period of time and react with or without albefaction, if having, under a cloud have tumour cell, the method poor specificity, easily causes false sun.Iodine test is based on the glycogen PAS, and specificity is lower equally.
Patent CN103808551A utilizes siphonage by solution storage in cotton swab bar, and in transportation, solution easily oozes out and is oxidized, and during use, dyeing liquor flows out slowly, operation inconvenience.
Chinese patent: what mention adjustment dyeing liquor pH employing in 2014100628449 is acetic acid, we study discovery, when the amount of acetic acid reaches 3%-5%, easily very easily be oxidized, and pH value also drops to about 3, do not meet the requirement of this dyeing liquor pH5.0-6.0, if acetic acid content does not reach 3%-5%, just cannot realize acetic acid albefaction reaction, cotton swab dyeing can not be realized and locate with tumor tissues acetic acid albefaction reaction dual.
Summary of the invention
For the problems referred to above, the object of the present invention is to provide a kind of detection sensitivity high, testing result is reliably for the detection reagent of epithelial neoplasms cell, and a kind of omnidistance closed injection-type is provided, the bad smell avoiding solution itself special is to the stimulation of operator, shorten the time of solution ingress of air simultaneously, avoid Quick Oxidation to cause false positive results, fully ensure the detection method of testing result accuracy.
For solving the problems of the technologies described above, the first technical scheme provided by the invention is such:
This is used for the detection reagent of epithelial neoplasms cell, described reagent is made up of the component of following weight percentage: the methylene blue of 0.03%-2%, 5%-10% fructose, 0.1%-1% ascorbic acid, 1%-5% folic acid, 40% dimethyl sulfoxide (DMSO), 3%-5% acetic acid-sodium acetate buffer solution, 0.1%-1% surfactant, and surplus is purified water.
Further, the above-mentioned detection reagent for epithelial neoplasms cell, the pH value of described 3%-5% acetic acid-sodium acetate buffer solution is 5.5 ± 0.5.
Further, the above-mentioned detection reagent for epithelial neoplasms cell, described surfactant is Tween-20.
For preparing this detection reagent, another one technical scheme of the present invention is such:
The preparation method of the above-mentioned detection reagent for epithelial neoplasms cell, comprises the steps: successively
1) each component is taken by percent by weight according to claim 1;
2) by step 1) folic acid that takes dissolves in dimethyl sulfoxide (DMSO), stirring and dissolving;
3) methylene blue is added to step 2) in solution, continue after stirring and dissolving to stir 40-80min;
4) surfactant is added step 3) in solution, stir and evenly mix;
5) fructose and ascorbic acid and alkaline aqueous solution are added to step 4) stirring and dissolving again in solution;
6) by acetic acid-sodium acetate buffer solution by being added to step 5) stir in solution and get final product.
In order to better use the present invention, the present invention also has a technical scheme to be such:
The detection method of the above-mentioned detection reagent for epithelial neoplasms cell, the method adopts following detection components to sample when detecting after, cotton swab insertion rod end is plugged on the access tube end of the reagent bottle that detection reagent according to claim 1 is housed, be inverted extrusion bottle, the cotton swab head after making detection reagent flow to sampling;
Described detection components comprises a reagent bottle and cotton swab matching used with reagent bottle, described reagent bottle comprises the soft bottle of shape of threads, bottle is provided with cylindrical hollow access tube, described bottle and access tube one-body molded, described cotton swab comprises a hollow insertion rod, the end, one end of insertion rod is provided with cucurbit head cotton head, and the external diameter of described insertion rod is identical with the internal diameter of access tube.
As a further improvement on the present invention, the detection method of the above-mentioned detection reagent for epithelial neoplasms cell, described access tube is also provided with the bottle stopper of sealed interface end.
As a further improvement on the present invention, the detection method of the above-mentioned detection reagent for epithelial neoplasms cell, described cotton swab outside is also provided with the packaging bag of sealing cotton swab.
As a further improvement on the present invention, the detection method of the above-mentioned detection reagent for epithelial neoplasms cell, the length 160-220mm of described insertion rod, diameter 3-6mm.
As a further improvement on the present invention, the detection method of the above-mentioned detection reagent for epithelial neoplasms cell, described cotton head tip diameter is 2.5-3.5mm, and the latter half diameter is 7-12mm, and the latter half is connected with insertion rod.
Compared with prior art, reductibility methylene blue and folic acid combine by the present invention, enter cancerous tumor cell by folacin receptor mediated.Change and quick position and detection epithelial tumor cell by dyeing and color occurring.The tip Pear-Shaped cotton swab that the present invention adopts, according to the design of woman uterus anatomical structure; Tip can stretch into the sampling of uterine neck mouth, and Sampling Area is larger, can improve and detect positive rate.Use sealed plastic bottle to store dyeing liquor, convenient transportation, can combine dexterously with cotton swab, and omnidistance closing is injected, and relative to directly dipping solution, the bad smell that solution itself can be avoided special is to the stimulation of operator; Shorten the time of solution ingress of air simultaneously, avoid Quick Oxidation to cause false positive results, fully ensure the accuracy of testing result.Folic acid is easily separated out in acid condition, and when reduce in the basic conditions methylene blue time, methylene blue indissoluble solution, the present invention adds proper amount of surfactant can promote each component dissolves, makes reaction more abundant; And acetic acid is changed into the acetic acid-sodium acetate buffer solution of pH5.0, can effectively address these problems.We are by 602 routine clinical sample checkings, and take pathological diagnosis as goldstandard, dyeing liquor of the present invention and TCT check contrast difference's not statistically significant.
Accompanying drawing explanation
Fig. 1 is detection components structural representation provided by the invention;
Fig. 2 is detection components assembled state schematic diagram of the present invention;
Fig. 3 detection components using state of the present invention schematic diagram.
Embodiment
Be described in further detail claim of the present invention below in conjunction with embodiment, but the present invention is not limited to this, the amendment of anyone limited number of time made within the scope of the claims in the present invention is still within the claims in the present invention protection domain.
Embodiment 1
A kind of detection reagent for epithelial neoplasms cell of the present invention, described reagent is made up of the component of following weight percentage: the methylene blue of 2g, 5g fructose, 1g ascorbic acid, 1g folic acid, 40g dimethyl sulfoxide (DMSO), 5g pH value are 5.5 acetic acid-sodium acetate buffer solution, 0.1g Tween-20, and surplus is purified water.
Embodiment 2
A kind of detection reagent for epithelial neoplasms cell of the present invention, described reagent is made up of the component of following weight percentage: the methylene blue of 0.03g, 10g fructose, 0.1g ascorbic acid, 5g folic acid, 40g dimethyl sulfoxide (DMSO), 3g pH value are 5.5 acetic acid-sodium acetate buffer solution, 1g Tween-20, and surplus is purified water.
Embodiment 3
A kind of detection reagent for epithelial neoplasms cell of the present invention, described reagent is made up of the component of following weight percentage: the methylene blue of 0.06g, 7g fructose, the ascorbic acid of 0.5g, 3g folic acid, 40g dimethyl sulfoxide (DMSO), 4g pH value are 5.5 acetic acid-sodium acetate buffer solution, 0.5g Tween-20, and surplus is purified water.
Embodiment 4
A kind of detection reagent for epithelial neoplasms cell of the present invention, described reagent is made up of the component of following weight percentage: the methylene blue of 1g, 8g fructose, the ascorbic acid of 0.3g, 2g folic acid, 40g dimethyl sulfoxide (DMSO), 3.5g pH value are 5.5 acetic acid-sodium acetate buffer solution, 0.3g Tween-20, and surplus is purified water.
In embodiment 1 to 4, any one is for the preparation method of the detection reagent of epithelial neoplasms cell, comprises the steps: successively
1) each component is taken by any one number of components in embodiment 1 to 4;
2) by step 1) folic acid that takes dissolves in dimethyl sulfoxide (DMSO), stirring and dissolving;
3) methylene blue is added to step 2) in solution, continue after stirring and dissolving to stir 40-80min;
4) surfactant is added step 3) in solution, stir and evenly mix;
5) fructose and ascorbic acid and alkaline aqueous solution are added to step 4) stirring and dissolving again in solution;
6) by acetic acid-sodium acetate buffer solution by being added to step 5) stir in solution and get final product.
In embodiment 1 to 4, any one is for the preparation method of the detection reagent of epithelial neoplasms cell, the method adopts following detection components to sample when detecting after, the insertion rod end of cotton swab is plugged on the access tube end of reagent bottle, built with in embodiment 1 to 4, any one detects reagent to described reagent bottle, then extrusion bottle is inverted, the cotton swab head after making detection reagent flow to sampling;
Described detection components comprises the reagent bottle 1 of a plastic material and cotton swab 2 matching used with reagent bottle 1, described reagent bottle 1 comprises the soft bottle 11 of shape of threads, bottle 11 is provided with cylindrical hollow access tube 12, access tube 12 is also provided with the bottle stopper 13 of sealed interface end.
Described bottle 11 is one-body molded with access tube 12, and described cotton swab 2 comprises a hollow insertion rod 21, and the end, one end of insertion rod 21 is provided with cucurbit head cotton 22, and the external diameter of described insertion rod 21 is identical with the internal diameter of access tube 12.
In particular, the length 160-220mm of described insertion rod 21, diameter 3-6mm, described cotton 22 tip diameter are 2.5-3.5mm, and the latter half diameter is 712mm, and lower part is connected with insertion rod 21.
Cotton swab outside is also provided with the packaging bag 3 of sealing cotton swab.
When not needing to use, detect reagent and load in reagent bottle 1, cover bottle stopper sealing; Cotton swab 2 loads packaging bag 3 and seals.Convenient storage and transport.
During use, open the packaging bag 3 of cotton swab 2, then open the bottle stopper of reagent bottle 1, the end of insertion rod 2 is plugged in reagent bottle 1 access tube, be inverted reagent bottle and extrude, making the reagent in reagent bottle flow into the cotton head of cotton swab, detecting with a cotton 22 tip sampling for cotton swab 2.
In order to advantage of the present invention is better described, provide conventional reagent testing result comparing result on detection solution testing result provided by the invention and market below.
The results contrast of table 1 modified form acetic acid white solution and TCT screening results and pathological diagnosis
Claims (9)
1. the detection reagent for epithelial neoplasms cell, it is characterized in that, described reagent is made up of the component of following weight percentage: the methylene blue of 0.03%-2%, 5%-10% fructose, 0.1%-1% ascorbic acid, 1%-5% folic acid, 40% dimethyl sulfoxide (DMSO), 3%-5% acetic acid-sodium acetate buffer solution, 0.1%-1% surfactant, and surplus is purified water.
2. the detection reagent for epithelial neoplasms cell according to claim 1, is characterized in that, the pH value of described 3%-5% acetic acid-sodium acetate buffer solution is 5.5 ± 0.5.
3. the detection reagent for epithelial neoplasms cell according to claim 1, is characterized in that, described surfactant is Tween-20.
4. the preparation method of the detection reagent for epithelial neoplasms cell according to claim 1, is characterized in that, comprise the steps: successively
1) each component is taken by percent by weight according to claim 1;
2) by step 1) folic acid that takes dissolves in dimethyl sulfoxide (DMSO), stirring and dissolving;
3) methylene blue is added to step 2) in solution, continue after stirring and dissolving to stir 40-80min;
4) surfactant is added step 3) in solution, stir and evenly mix;
5) fructose and ascorbic acid and alkaline aqueous solution are added to step 4) stirring and dissolving again in solution;
6) by acetic acid-sodium acetate buffer solution by being added to step 5) stir in solution and get final product.
5. the detection method of the detection reagent for epithelial neoplasms cell according to claim 1, it is characterized in that, the method adopts following detection components to sample when detecting after, the insertion rod end of cotton swab is plugged on the access tube end of the reagent bottle that detection reagent according to claim 1 is housed, be inverted extrusion bottle, the cotton swab head after making detection reagent flow to sampling;
Described detection components comprise a reagent bottle (1) and with reagent bottle (1) matching used cotton swab (2), described reagent bottle (1) comprises the soft bottle of shape of threads (11), bottle (11) is provided with cylindrical hollow access tube (12), described bottle (11) is one-body molded with access tube (12), described cotton swab (2) comprises a hollow insertion rod (21), the end, one end of insertion rod (21) is provided with cucurbit head cotton head (22), and the external diameter of described insertion rod (21) is identical with the internal diameter of access tube (12).
6. the detection method of the detection reagent for epithelial neoplasms cell according to claim 5, is characterized in that, described access tube (12) is also provided with the bottle stopper (13) of sealed interface end.
7. the detection method of the detection reagent for epithelial neoplasms cell according to claim 5, is characterized in that, described cotton swab (2) outside is also provided with the packaging bag (3) of sealing cotton swab.
8. the detection method of the detection reagent for epithelial neoplasms cell according to claim 5, is characterized in that, the length 160mm-220mm of described insertion rod (21), diameter 3mm-6mm.
9. the detection method of the detection reagent for epithelial neoplasms cell according to claim 5, it is characterized in that, described cotton head (22) tip diameter is 2.5-3.5mm, and the latter half diameter is 7mm-12mm, and the latter half is connected with insertion rod (21).
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| CN201410854634.3A CN104483473B (en) | 2014-12-31 | 2014-12-31 | For detection reagent and the preparation and determination methods method of epithelial neoplasms cell |
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| CN201410854634.3A CN104483473B (en) | 2014-12-31 | 2014-12-31 | For detection reagent and the preparation and determination methods method of epithelial neoplasms cell |
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| CN104483473B CN104483473B (en) | 2016-03-02 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108318478A (en) * | 2017-01-18 | 2018-07-24 | 四川天莆高科医疗器械有限公司 | A kind of detection reagent and its preparation method and application of human epithelia's cancer cell |
| CN110455602A (en) * | 2019-07-18 | 2019-11-15 | 浙江中法制药有限公司 | Specific stain liquid and its preparation method and application based on cast-off cells dyeing |
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