CN104480902A - Concrete dam joint leaking stoppage method - Google Patents

Concrete dam joint leaking stoppage method Download PDF

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Publication number
CN104480902A
CN104480902A CN201410671522.4A CN201410671522A CN104480902A CN 104480902 A CN104480902 A CN 104480902A CN 201410671522 A CN201410671522 A CN 201410671522A CN 104480902 A CN104480902 A CN 104480902A
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China
Prior art keywords
leakage
stop liquid
leaking
liquid
urea
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CN201410671522.4A
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Chinese (zh)
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CN104480902B (en
Inventor
李明东
李琳
田安国
李驰
张志峰
朱丽萍
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Huaihai Institute of Techology
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Huaihai Institute of Techology
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Priority to CN201410671522.4A priority Critical patent/CN104480902B/en
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    • EFIXED CONSTRUCTIONS
    • E02HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02BHYDRAULIC ENGINEERING
    • E02B3/00Engineering works in connection with control or use of streams, rivers, coasts, or other marine sites; Sealings or joints for engineering works in general
    • E02B3/16Sealings or joints
    • EFIXED CONSTRUCTIONS
    • E02HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02DFOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D15/00Handling building or like materials for hydraulic engineering or foundations

Abstract

The invention relates to a concrete dam joint leaking stoppage method. According to the method, a leaking stoppage device is used, when leaking is stopped, a leaking stoppage liquid storage container is placed at the highest position of a dam crest or a leaking joint, a leaking stoppage liquid collection pool is placed at the lowest position of the leaking joint, a liquid outlet of a drain pipe is formed at the highest position of the leaking joint, leaking stoppage liquid is filled in the leaking stoppage liquid storage container and aerated by turning on an aerator, a drain valve is opened after 5-30 minutes, the leaking stoppage liquid flows in from the highest point of the leaking joint, the size of the valve is adjusted, so that the flow of the leaking stoppage liquid is slightly lower than that of the leaking joint, a recovery pump is turned on after the leaking stoppage liquid flows out from the leaking stoppage liquid collection pool, and the cycle continues until the joint is plugged. Microorganism leaking stoppage liquid is mineralized to stop leaking, is fine in flowability and easily penetrates into a dam, the flow path of the leaking stoppage liquid is a leaking passage, and fine leaking joints and leaking joints with thin upstream and thick downstream can be stopped from leaking.

Description

A kind of blocking method of concrete dam gap
Technical field
The present invention relates to a kind of method of concrete dam leak stopping, particularly use leakage-stop liquid concrete dam gap to be carried out to the method for leak stopping.
Background technology
Concrete dam can produce seepage seam in the situations such as differential settlement, thermal stresses, constructional deficiency, causes a large amount of water storage to lose.Current blocking method mostly is and pours into the material such as cement paste, pitch, because leakage-stop liquid mobility is limited, is often difficult to the trickle seepage seam going deep into dam body inside.Ultra-fine grain deep enough dam body of energy under flow action is inner, but the seepage seam thin for upstream, downstream is thick then can not play good plugging effect.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, provides a kind of blocking method simple, easy to operate, the blocking method in the concrete dam gap of leakage-stop liquid good fluidity.
Technical problem to be solved by this invention is realized by following technical scheme.The present invention is a kind of blocking method of concrete dam gap, be characterized in, the method uses device for plugging, described device for plugging comprises leakage-stop liquid storage pond, aerator and leakage-stop liquid collecting pit, the loose gas impeller of described aerator is located in leakage-stop liquid storage pond, and the side in leakage-stop liquid storage pond is provided with liquid discharging tube, and opposite side is provided with the recovery tube be communicated with leakage-stop liquid collecting pit, described liquid discharging tube is equipped with bleeder valve, recovery tube is equipped with recovery pump; During leak stopping operation: leakage-stop liquid is stored up the highest point that pond is placed on dam crest or seepage seam, leakage-stop liquid collecting pit is placed on the lowest part of seepage seam, and the liquid outlet of liquid discharging tube is located at the peak place of seepage seam; Leakage-stop liquid storage is equipped with leakage-stop liquid in pond, open aerator and aeration is carried out to leakage-stop liquid, after 5 ~ 30 minutes, opening bleeder valve makes leakage-stop liquid flow into from seepage seam peak, and control valve size, make leakage-stop liquid flow be slightly less than seepage seam flow, after leakage-stop liquid flows out from leakage-stop liquid collecting pit, open recovery pump; Such cycling is until gap is plugged.
In the blocking method in concrete dam gap of the present invention, the leakage-stop liquid volume of described leakage-stop liquid liquid storage tank is 0.5 ~ 1 hour seepage seam flow; The volume of described leakage-stop liquid collecting pit is 5 ~ 10 minutes seepage seam flows.
In the blocking method in concrete dam gap of the present invention, described leakage-stop liquid take water as solvent and contains calcium ion, urea and urea bacterium; Wherein: the mass concentration of urea is 15-90g/L, in calcium ion and urea, the number ratio of carbon atom is 0.7 ~ 1.5:1, and the concentration of urea bacterium is 10 8~ 10 10individual/L; And the pH of leakage-stop liquid is 5.0 ~ 7.0.
In the blocking method in concrete dam gap of the present invention, described calcium ion, from soluble calcium salt, comprises calcium chloride or calcium nitrate.
In the blocking method in concrete dam gap of the present invention, also containing the nutriment for bacterial growth breeding that mass concentration is 5 ~ 75 g/L in described leakage-stop liquid.
In the blocking method in concrete dam gap of the present invention, described nutriment be selected from the yeast extract of 5 ~ 30 g/L, the beef powder of 3 ~ 20g/L, the sweet mellow wine of 10 ~ 50g/L or the soy peptone of 10 ~ 40g/L one or more.
In the blocking method in concrete dam gap of the present invention, the pH value 5.5 ~ 6.5 of described leakage-stop liquid.
In the blocking method in concrete dam gap of the present invention, described urea bacterium for the raw spore sarcine of Pasteur ( sporosarcina pasteurii) CGMCC No. 1.3687; Or ground urea bacillus ( ureibacillus terrenus) CGMCC No. 1.7272.
Bacterial strain Pasteur involved in the present invention is raw, and spore sarcine (Sporosarcina pasteurii) is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, and deposit number is CGMCC No. 1.3687; Depositary institution address: Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, phone: 010-64807355.
Bacterial strain ground urea bacillus (Ureibacillus terrenus) involved in the present invention is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, and deposit number is CGMCC No. 1.7272; Depositary institution address: Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, phone: 010-64807355.
In the blocking method in concrete dam gap of the present invention, the preparation process of leakage-stop liquid is as follows:
(1) urea bacterium is cultivated according to a conventional method rear for subsequent use;
(2) in often liter of deionized water, add the urea of 0.25-1.5mol calcium chloride or calcium nitrate, 0.25-1.5mol; Add nutriment on demand again, dissolving mixes;
(3) be 10 by the concentration of urea bacterium 8~ 10 10individual/L adds urea bacterium wherein; Obtain leakage-stop liquid.
The conventional method of urea bacterium is for first to prepare fluid nutrient medium, and the preparation method of fluid nutrient medium is, by 20g yeast extract, 10g ammonium sulfate dissolves in 1000ml deionized water, then uses alkaline buffer adjusted to ph to 9.0, then autoclave sterilization.
Then carry out Bacteria Culture, method is (1) inoculation: add 25ml nutrient solution at culture tube, add 250ul bacterial classification with liquid-transfering gun; (2) cultivate: be placed on shaking table and shake up 40h; (3) outwell waste liquid after centrifugal, then add 25ml culture medium, shake up.After cultivating, concentration is about 10 9individual/ml (order of magnitude, i.e. 1-9*), bacterium preserves: 4 degree of Refrigerator stores.
The urea of 1.0mol calcium chloride or calcium nitrate, 0.8mol is added in often liter of deionized water; Add nutriment on demand again, dissolving mixes; Be 10 by the concentration of urea bacterium 8~ 10 10individual/L adds urea bacterium wherein; Obtain leakage-stop liquid.
The operating principle of leakage-stop liquid
Bacterium+Ca 2+→ bacterium-Ca 2+(1)
Bacteria lives produces urease
Urease catalyzes CO (NH 2) 2+ 2H 2o → CO 3 2-+ 2NH 4 +(2)
Bacterium-Ca 2++ CO 3 2-→ bacterium-CaCO 3(3)
The calcium carbonate generated is based on calcite, has cementing properties.
Compared with prior art, the present invention carries out leak stopping, its good fluidity by using microorganism leakage-stop liquid mineralising, and the flow process of leakage-stop liquid is leakage passage, is easy to go deep into dam body inside, energy all kinds of crack of shutoff, the crack that especially the thin downstream of micro-cracks and upstream is thick.This method applicability is strong, can be applicable to the various gaps of concrete dam, be particularly useful for the seepage seam that upstream is thin, downstream is thick, and leakage-stop liquid environmental protection can not to environment.
Accompanying drawing explanation
Fig. 1 is the leak stopping structure schematic diagram of a kind of wire seepage seam of the present invention;
Fig. 2 is that seepage seam is from the through structural representation at the bottom of dam of dam crest.
Detailed description of the invention
Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Embodiment 1, a kind of blocking method of concrete dam gap, as shown in Figure 1, when concrete dam 1 seepage seam 8 is wire, extends through opposite side from the side of concrete dam 1.At this moment blocking method is, the method uses device for plugging, described device for plugging comprises leakage-stop liquid storage pond 4, aerator 3 and leakage-stop liquid collecting pit 7, the loose gas impeller of described aerator 3 is located in leakage-stop liquid storage pond 4, the side in leakage-stop liquid storage pond 4 is provided with liquid discharging tube 2, opposite side is provided with the recovery tube 6 be communicated with leakage-stop liquid collecting pit 7, described liquid discharging tube 2 is equipped with bleeder valve, recovery tube 6 is equipped with recovery pump 5; During leak stopping operation: leakage-stop liquid is stored up pond 4 and be placed on dam crest, leakage-stop liquid collecting pit 7 is placed on the lowest part of seepage seam 8, and the liquid outlet of liquid discharging tube 2 is located at the peak place of seepage seam 8; Leakage-stop liquid storage is equipped with leakage-stop liquid in pond 4, open aerator 3 pairs of leakage-stop liquids and carry out aeration, after 5 ~ 30 minutes, opening bleeder valve makes leakage-stop liquid stitch 8 peaks inflows from seepage, and control valve size, make leakage-stop liquid flow be slightly less than seepage seam flow, after leakage-stop liquid flows out from leakage-stop liquid collecting pit 7, open recovery pump 5; Leakage-stop liquid on stream hydrolysis of urea reaction generate carbonate and calcium ion react the calcium carbonate generating and there is cementing properties, be bonded on the wall in gap, block gap gradually, leakage-stop liquid repeatedly flows through gap until gap is plugged.Halfway, if plugging effect not obvious after, change leakage-stop liquid continue operation.
Embodiment 2, a kind of blocking method of concrete dam gap, as shown in Figure 2, when concrete dam 1 seepage seam 8 is wire, the through bottom to dam from the top of concrete dam 1.At this moment blocking method is, the method uses device for plugging, described device for plugging comprises leakage-stop liquid storage pond 4, aerator 3 and leakage-stop liquid collecting pit 7, the loose gas impeller of described aerator 3 is located in leakage-stop liquid storage pond 4, the side in leakage-stop liquid storage pond 4 is provided with liquid discharging tube 2, opposite side is provided with the recovery tube 6 be communicated with leakage-stop liquid collecting pit 7, described liquid discharging tube 2 is equipped with bleeder valve, recovery tube 6 is equipped with recovery pump 5; During leak stopping operation: highest point aerator 3 and leakage-stop liquid storage pond 4 being placed on seepage seam 8, leakage-stop liquid collecting pit 7 is placed on the lowest part of seepage seam 8, and the liquid outlet of liquid discharging tube 2 is located at the peak place of seepage seam 8; Leakage-stop liquid storage is equipped with leakage-stop liquid in pond 4, open aerator 3 pairs of leakage-stop liquids and carry out aeration, after 5 ~ 30 minutes, opening bleeder valve makes leakage-stop liquid stitch 8 peaks inflows from seepage, and control valve size, make leakage-stop liquid flow be slightly less than seepage seam flow, after leakage-stop liquid flows out from leakage-stop liquid collecting pit 7, open recovery pump 5; Such circulation is until gap is plugged.Halfway, if plugging effect not obvious after, change leakage-stop liquid continue operation.
Embodiment 3, the method as described in embodiment 1 and 2, the leakage-stop liquid volume of described leakage-stop liquid liquid storage tank is 0.5 ~ 1 hour seepage seam flow; The volume of described leakage-stop liquid collecting pit 7 is 5 ~ 10 minutes seepage seam flows.
Embodiment 4, the method as described in embodiment 1 and 2, described leakage-stop liquid take water as solvent and contains calcium ion, urea and urea bacterium; Wherein: the mass concentration of urea is 50g/L, in calcium ion and urea, the number ratio of carbon atom is 1:1, and the concentration of urea bacterium is 10 8~ 10 10individual/L; And the pH of leakage-stop liquid is 6.0.
Embodiment 5, method as described in Example 4, described calcium ion, from soluble calcium salt, comprises calcium chloride or calcium nitrate.
Embodiment 6, method as described in Example 4: also containing the nutriment for bacterial growth breeding that mass concentration is 60 g/L in described leakage-stop liquid.
Embodiment 7, method as described in Example 4, described nutriment be selected from the yeast extract of 20 g/L, the beef powder of 12g/L, the sweet mellow wine of 30g/L or the soy peptone of 25g/L one or more.
Embodiment 8, method as described in Example 4, the pH value of described leakage-stop liquid is 5.5 ~ 6.5.
Embodiment 9, the method as described in embodiment 1-8, described urea bacterium for the raw spore sarcine of Pasteur ( sporosarcina pasteurii) CGMCC No. 1.3687; Or ground urea bacillus ( ureibacillus terrenus) CGMCC No. 1.7272.
Embodiment 10, the purposes of the leakage-stop liquid according to embodiment 1 in building or structure leak stopping.
Embodiment 11, the method as described in embodiment 1-9, the preparation process of described leakage-stop liquid is as follows:
(1) urea bacterium is cultivated according to a conventional method rear for subsequent use;
(2) in often liter of deionized water, add the urea of 0.8mol calcium chloride or calcium nitrate, 0.8mol; Add nutriment on demand again, dissolving mixes;
(3) be 10 by the concentration of urea bacterium 8~ 10 10individual/L adds urea bacterium wherein; Obtain leakage-stop liquid.

Claims (9)

1. the blocking method in a concrete dam gap, it is characterized in that, the method uses device for plugging, described device for plugging comprises leakage-stop liquid storage pond, aerator and leakage-stop liquid collecting pit, the loose gas impeller of described aerator is located in leakage-stop liquid storage pond, and the side in leakage-stop liquid storage pond is provided with liquid discharging tube, and opposite side is provided with the recovery tube be communicated with leakage-stop liquid collecting pit, described liquid discharging tube is equipped with bleeder valve, recovery tube is equipped with recovery pump; During leak stopping operation: leakage-stop liquid is stored up the highest point that pond is placed on dam crest or seepage seam, leakage-stop liquid collecting pit is placed on the lowest part of seepage seam, and the liquid outlet of liquid discharging tube is located at the peak place of seepage seam; Leakage-stop liquid storage is equipped with leakage-stop liquid in pond, open aerator and aeration is carried out to leakage-stop liquid, after 5 ~ 30 minutes, opening bleeder valve makes leakage-stop liquid flow into from seepage seam peak, and control valve size, make leakage-stop liquid flow be slightly less than seepage seam flow, after leakage-stop liquid flows out from leakage-stop liquid collecting pit, open recovery pump; Such circulation is until gap is plugged.
2. the blocking method in concrete dam gap according to claim 1, is characterized in that, the leakage-stop liquid volume of described leakage-stop liquid liquid storage tank is 0.5 ~ 1 hour seepage seam flow; The volume of described leakage-stop liquid collecting pit is 5 ~ 10 minutes seepage seam flows.
3. the blocking method in concrete dam gap according to claim 1, is characterized in that, described leakage-stop liquid take water as solvent and contains calcium ion, urea and urea bacterium; Wherein: the mass concentration of urea is 15-90g/L, in calcium ion and urea, the number ratio of carbon atom is 0.7 ~ 1.5:1, and the concentration of urea bacterium is 10 8~ 10 10individual/L; And the pH of leakage-stop liquid is 5.0 ~ 7.0.
4. the blocking method in concrete dam gap according to claim 3, is characterized in that: described calcium ion, from soluble calcium salt, comprises calcium chloride or calcium nitrate.
5. the blocking method in concrete dam gap according to claim 3, is characterized in that: also containing the nutriment for bacterial growth breeding that mass concentration is 5 ~ 75 g/L in described leakage-stop liquid.
6. the blocking method in concrete dam gap according to claim 3, is characterized in that: described nutriment be selected from the yeast extract of 5 ~ 30 g/L, the beef powder of 3 ~ 20g/L, the sweet mellow wine of 10 ~ 50g/L or the soy peptone of 10 ~ 40g/L one or more.
7. the blocking method in concrete dam gap according to claim 3, is characterized in that: the pH value of described leakage-stop liquid is 5.5 ~ 6.5.
8. the blocking method in the concrete dam gap according to any item of claim 3-7, is characterized in that, described urea bacterium for the raw spore sarcine of Pasteur ( sporosarcina pasteurii) CGMCC No. 1.3687; Or ground urea bacillus ( ureibacillus terrenus) CGMCC No. 1.7272.
9. the blocking method in the concrete dam gap according to any one of claim 1-8, is characterized in that: the preparation process of described leakage-stop liquid is as follows:
(1) urea bacterium is cultivated according to a conventional method rear for subsequent use;
(2) in often liter of deionized water, add the urea of 0.25-1.5mol calcium chloride or calcium nitrate, 0.25-1.5mol; Add nutriment on demand again, dissolving mixes;
(3) be 10 by the concentration of urea bacterium 8~ 10 10individual/L adds urea bacterium wherein; Obtain leakage-stop liquid.
CN201410671522.4A 2014-11-21 2014-11-21 A kind of blocking method in concrete dam gap Expired - Fee Related CN104480902B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116024979A (en) * 2023-03-31 2023-04-28 水利部交通运输部国家能源局南京水利科学研究院 Panel dam leakage repairing equipment and method

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001288731A (en) * 2000-04-04 2001-10-19 So Sekkei:Kk Structure of dam and water quality purifying method for river water
CN1587536A (en) * 2004-07-15 2005-03-02 国家电力公司华东勘测设计研究院 Back ward water draining system of face plate rock-fill dam dam body and its construction method
CN102733355A (en) * 2012-06-25 2012-10-17 中国葛洲坝集团国际工程有限公司 Device for performing dam front underwater leaking stoppage by using ultrafine particles and method for performing leaking stoppage
CN103074869A (en) * 2013-02-05 2013-05-01 张永忠 Dam leakage stopping method
CN103174149A (en) * 2013-03-28 2013-06-26 葛洲坝集团试验检测有限公司 Dam body joint circulation grouting method
CN104131532A (en) * 2014-07-28 2014-11-05 中国电建集团贵阳勘测设计研究院有限公司 Leaking stoppage method and structure for karst pipeline

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001288731A (en) * 2000-04-04 2001-10-19 So Sekkei:Kk Structure of dam and water quality purifying method for river water
CN1587536A (en) * 2004-07-15 2005-03-02 国家电力公司华东勘测设计研究院 Back ward water draining system of face plate rock-fill dam dam body and its construction method
CN102733355A (en) * 2012-06-25 2012-10-17 中国葛洲坝集团国际工程有限公司 Device for performing dam front underwater leaking stoppage by using ultrafine particles and method for performing leaking stoppage
CN103074869A (en) * 2013-02-05 2013-05-01 张永忠 Dam leakage stopping method
CN103174149A (en) * 2013-03-28 2013-06-26 葛洲坝集团试验检测有限公司 Dam body joint circulation grouting method
CN104131532A (en) * 2014-07-28 2014-11-05 中国电建集团贵阳勘测设计研究院有限公司 Leaking stoppage method and structure for karst pipeline

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116024979A (en) * 2023-03-31 2023-04-28 水利部交通运输部国家能源局南京水利科学研究院 Panel dam leakage repairing equipment and method

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