CN104472674B - A kind of antistaling agent for litchi and preparation method thereof - Google Patents
A kind of antistaling agent for litchi and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of antistaling agent for litchi and preparation method thereof.The antistaling agent, by weight percentage, its formula composition is as follows:Flos Lonicerae extract 4 ~ 8%, Radix Glycyrrhizae extract 2 ~ 3%, Fructus Litchi peel extract 1 ~ 3%, calcium chloride 0.8 ~ 1.2%, glycerol 1.5 ~ 2%, shitosan 0.2 ~ 0.6%, gibberellins 0.015 ~ 0.02%, dehydroactic acid sodium 0.8 ~ 1.2%, remaining are water.The invention has the beneficial effects as follows:1st, main component is Chinese medicine extraction composition formula, safer to lichee fresh-keeping;2nd, Flos Lonicerae is through high hydrostatic pressure extraction, secondary separation, and the extract for preparing can greatly preserve the effective ingredient of Flos Lonicerae, improves fresh-keeping efficiency;3rd, Radix Glycyrrhizae extract is prepared using supercritical extraction method, effectively the effective ingredient in extraction Radix Glycyrrhizae;4th, litchi rind adopts homogenate extraction method, and extraction time is short, and extract has preferable bacteriostasis;5th, of good preservation effect, fresh keeping time is long.
Description
Technical field
The invention belongs to field of fruit freshness keeping, is related to a kind of antistaling agent for litchi and preparation method thereof.
Background technology
The ripe harvesting of high temperature season in full summer more than Fructus Litchi, post-harvest fruits physiological metabolism are vigorous, and fresh fruit easily brown stain is rotted, sternly
Ghost image rings its commodity value.China's Fructus Litchi commercialized treatment after adopting is relative with storage technique equipment to fall behind, and main storage is protected
Fresh method includes room temperature storage, cryopreservation, low temperature controlled atmosphere, freezing Storage, chemical treatment, heat treatment and radiation etc., this
The shortcoming of a little methods is can not to remove the black color of peel, fruit color to shade, sell that difference, shelf life and cold fresh phase be short, sulfur dioxide is residual
Stay, limit fresh fruit sale.
Content of the invention
Instant invention overcomes above-mentioned deficiency, there is provided a kind of with the antistaling agent for litchi being prepared from based on Chinese medicine ingredients
And preparation method thereof.
A kind of antistaling agent for litchi, by weight percentage, its formula composition is as follows:
Flos Lonicerae extract 4 ~ 8%, Radix Glycyrrhizae extract 2 ~ 3%, Fructus Litchi peel extract 1 ~ 3%, calcium chloride 0.8 ~ 1.2%, the third three
Alcohol 1.5 ~ 2%, shitosan 0.2 ~ 0.6%, gibberellins 0.015 ~ 0.02%, dehydroactic acid sodium 0.8 ~ 1.2%, remaining are water.
The method of above-mentioned antistaling agent for litchi, including following step:
1)The preparation of Flos Lonicerae extract:
Flos Lonicerae is put into hyperpressure extraction tank, ethanol is added, pressurizes and keep pressure, pressure release to filter, filtrate cold preservation,
Filtrate first time centrifugation after cold preservation, collects supernatant, and cold preservation again, second centrifugation are collected supernatant and is obtained
Flos Lonicerae extract;
2)The preparation of Radix Glycyrrhizae extract:
Weigh powder Radix Glycyrrhizae to be put in extraction kettle, be passed through CO2As fluid is extracted, surpassed using ethanol as entrainer
Critical extraction is obtained Radix Glycyrrhizae extract;
3)The preparation of Fructus Litchi peel extract:
Weigh powder litchi rind to be put in flash extracter, Fructus Litchi peel extract is obtained as extractant with 95% ethanol;
4)The preparation of antistaling agent:
Flos Lonicerae extract, Radix Glycyrrhizae extract, calcium chloride, glycerol, gibberellins and dehydroactic acid sodium are weighed by formula ratio,
Boiling water dissolves, shitosan needed for adding formula ratio while hot, stirs to being completely dissolved, is cooled to room temperature.
Preferably, the step 1)In, 5 ~ 8 times for Flos Lonicerae weight of amount of alcohol added.
Preferably, the step 1)In, moulding pressure is 100 ~ 400Mpa, and pressure hold time is 10 ~ 15min.
Preferably, the step 1)In, first time refrigerated storage temperature is 1 ~ 4 DEG C, and cold preservation time is 2 ~ 5h;Refrigerated storage temperature again
For 1-4 DEG C, cold preservation time is 1 ~ 3h.
Preferably, the step 1)In, the rotating speed of first time centrifugation is 3000 ~ 3500r/min, and disengaging time is 10
~15min;The rotating speed of second centrifugation is 5000 ~ 6000r/min, and disengaging time is 5 ~ 8min.
Preferably, the step 2)In, CO2Flow velocity is:15 ~ 18m/s, extracting pressure are 30 ~ 35Mpa, and extraction temperature is
35 ~ 45 DEG C, extraction time is 4 ~ 6h
Preferably, the step 3)In, solid-liquid ratio is 1: 10 ~ 1: 15, Extracting temperature be 70 ~ 80 DEG C, extraction time be 5 ~
10min.
The invention has the beneficial effects as follows:
1st, main component is Flos Lonicerae and Chinese medicine of licorice root formula, and integration of edible and medicinal herbs is safer to lichee fresh-keeping;
2nd, Flos Lonicerae through high hydrostatic pressure extraction, secondary separation, the extract for preparing can greatly preserve having for Flos Lonicerae
Effect composition, improves fresh-keeping efficiency;
3rd, Radix Glycyrrhizae extract is prepared using supercritical extraction method, and glycyrrhizic acid and flavone effectively in extraction Radix Glycyrrhizae etc. is effectively
Composition;
4th, Fructus Litchi peel extract has preferable bacteriostasis, can effectively improve the fresh-keeping effect of antistaling agent;
5th, of good preservation effect, fresh keeping time is long.
Specific embodiment
Below the preferably embodiment of the present invention is described in further detail:
Embodiment 1
Antistaling agent for litchi component, by weight percentage, its formula is consisted of:Flos Lonicerae extract 4%, Radix Glycyrrhizae extract 2%,
Fructus Litchi peel extract 1%, calcium chloride 0.8%, glycerol 1.5%, shitosan 0.2%, gibberellins 0.015%, dehydroactic acid sodium 0.8%,
Remaining is water.Preparation method:
1)The preparation of Flos Lonicerae extract:
Flos Lonicerae is put into hyperpressure extraction tank, adds 5 times of 95% ethanol of weight, moulding pressure to be 100Mpa and keep pressing
Power 12min, pressure release are filtered, filtrate first time cold preservation, and refrigerated storage temperature is 4 DEG C, and cold preservation time is 5h, the filtrate first after cold preservation
Secondary centrifugation, rotating speed are 3000r/min, and disengaging time is 15min, collect supernatant, again cold preservation, and refrigerated storage temperature is 1 DEG C,
Cold preservation time is 1h, and second centrifugation, rotating speed are 5000r/min, and disengaging time is 5min, collects supernatant and gold silver is obtained
Flower extract.
2)The preparation of Radix Glycyrrhizae extract:
Weigh powder Radix Glycyrrhizae to be put in extraction kettle, be passed through CO2As fluid is extracted, using ethanol as entrainer, CO2Stream
Speed is:18m/s, extracting pressure are 30Mpa, and extraction temperature is 40 DEG C, and extraction time is 4h, carry out supercritical extraction and Radix Glycyrrhizae is obtained
Extract.
3)The preparation of Fructus Litchi peel extract:
Weigh powder litchi rind to be put in flash extracter, with 95% ethanol as extractant, solid-liquid ratio is 1: 10, extract
Temperature is 80 DEG C, and extraction time is 5min, and Fructus Litchi peel extract is obtained.
4)The preparation of antistaling agent:
Flos Lonicerae extract, Radix Glycyrrhizae extract, calcium chloride, glycerol, gibberellins and dehydroactic acid sodium are weighed by formula ratio,
Boiling water dissolves, shitosan needed for adding formula ratio while hot, stirs to being completely dissolved, is cooled to room temperature, and antistaling agent for litchi is obtained.
Six ~ medium well, epidermis not damaged, the litchi fruits of no disease and pests harm of fine day harvesting are selected, the present embodiment is soaked into
In obtained antistaling agent after 1 ~ 2min, natural air drying is placed in 4 ~ 6 DEG C of temperature and preserves, and rots by Fructus Litchi or browned area exceedes
It is set to rotten fruit during whole fruit area 1/4, after preservation 15 days, determines denaturation index, healthy fruit, rotting rate, solubility solid
The indexs such as thing, measurement result are as shown in table 1:
1 fresh-keeping effect synopsis of table(Cryopreservation 15d)
After litchi fruits after blank group and the process of antistaling agent treatment group preserve 5d in 20 ~ 25 DEG C of room temperature, Fructus Litchi is determined
The indexs such as browning index, healthy fruit, rotting rate, soluble solid, measurement result are as shown in table 2:
2 fresh-keeping effect synopsis of table(Room temperature storage 5d)
As a result, after showing Fructus Litchi sample cryopreservation 15d processed using the antistaling agent, the sense organ excellent flavor of Fructus Litchi is brown
Varying index is 0.94, and rotting rate is 4.11%, and healthy fruit reaches 95.89%, and sarcocarp soluble solid is 17.4 Brix, fresh-keeping
Effect is substantially better than control fruit.After room temperature storage 5d, blank group is rotted substantially, and the Fructus Litchi quality that antistaling agent is processed is substantially better than
Blank group.
Embodiment 2
Antistaling agent for litchi component, by weight percentage, its formula is consisted of:Flos Lonicerae extract 6%, Radix Glycyrrhizae extract
2.5%, Fructus Litchi peel extract 2%, calcium chloride 1%, glycerol 1.8%, shitosan 0.4%, gibberellins 0.018%, dehydroactic acid sodium 1%,
Remaining is water.
Preparation method:
1)The preparation of Flos Lonicerae extract:
Flos Lonicerae is put into hyperpressure extraction tank, adds 6 times of 95% ethanol of weight, moulding pressure to be 400Mpa and keep pressing
Power 10min, pressure release are filtered, filtrate first time cold preservation, and refrigerated storage temperature is 1 DEG C, and cold preservation time is 2h, the filtrate first after cold preservation
Secondary centrifugation, rotating speed are 3500r/min, and disengaging time is 12min, collect supernatant, again cold preservation, and refrigerated storage temperature is 2 DEG C,
Cold preservation time is 3h, and second centrifugation, rotating speed are 5000r/min, and disengaging time is 8min, collects supernatant and gold silver is obtained
Flower extract.
2)The preparation of Radix Glycyrrhizae extract:
Weigh powder Radix Glycyrrhizae to be put in extraction kettle, be passed through CO2As fluid is extracted, using ethanol as entrainer, CO2Stream
Speed is:15m/s, extracting pressure are 35Mpa, and extraction temperature is 45 DEG C, and extraction time is 5h, carry out supercritical extraction and Radix Glycyrrhizae is obtained
Extract.
3)The preparation of Fructus Litchi peel extract:
Weigh powder litchi rind to be put in flash extracter, with 95% ethanol as extractant, solid-liquid ratio is 1: 15, extract
Temperature is 70 DEG C, and extraction time is 10min, and Fructus Litchi peel extract is obtained.
4)The preparation of antistaling agent:
Flos Lonicerae extract, Radix Glycyrrhizae extract, calcium chloride, glycerol, gibberellins and dehydroactic acid sodium are weighed by formula ratio,
Boiling water dissolves, shitosan needed for adding formula ratio while hot, stirs to being completely dissolved, is cooled to room temperature, and antistaling agent for litchi is obtained.
Six ~ medium well, epidermis not damaged, the litchi fruits of no disease and pests harm of fine day harvesting are selected, the present embodiment is soaked into
In obtained antistaling agent after 1 ~ 2min, natural air drying is placed in 4 ~ 6 DEG C of temperature and preserves, and rots by Fructus Litchi or browned area exceedes
It is set to rotten fruit during whole fruit area 1/4, after preservation 20 days, determines denaturation index, healthy fruit, rotting rate, solubility solid
The indexs such as thing, measurement result are as shown in table 3:
3 fresh-keeping effect synopsis of table(Cryopreservation 20d)
As a result, after showing Fructus Litchi sample cryopreservation 20d processed using the antistaling agent, the sense organ excellent flavor of Fructus Litchi is brown
Varying index is 1.39, and rotting rate is 9.88%, and healthy fruit reaches 90.12%, and sarcocarp soluble solid is 16.8 Brix, fresh-keeping
Effect is substantially better than control fruit.
Embodiment 3
Flos Lonicerae extract 8%, Radix Glycyrrhizae extract 3%, Fructus Litchi peel extract 3%, calcium chloride 1.2%, glycerol 2%, shitosan
0.6%, gibberellins 0.02%, dehydroactic acid sodium 1.2%, remaining are water.
Preparation method:
1)The preparation of Flos Lonicerae extract:
Flos Lonicerae is put into hyperpressure extraction tank, adds 8 times of 95% ethanol of weight, moulding pressure to be 300Mpa and keep pressing
Power 15min, pressure release are filtered, filtrate first time cold preservation, and refrigerated storage temperature is 2 DEG C, and cold preservation time is 4h, the filtrate first after cold preservation
Secondary centrifugation, rotating speed are 3500r/min, and disengaging time is 15min, collect supernatant, again cold preservation, and refrigerated storage temperature is 4 DEG C,
Cold preservation time is 3h, and second centrifugation, rotating speed are 6000r/min, and disengaging time is 8min, collects supernatant and gold silver is obtained
Flower extract.
2)The preparation of Radix Glycyrrhizae extract:
Weigh powder Radix Glycyrrhizae to be put in extraction kettle, be passed through CO2As fluid is extracted, using ethanol as entrainer, CO2Stream
Speed is:15m/s, extracting pressure are 35Mpa, and extraction temperature is 35 DEG C, and extraction time is 6h, carry out supercritical extraction and Radix Glycyrrhizae is obtained
Extract.
3)The preparation of Fructus Litchi peel extract:
Weigh powder litchi rind to be put in flash extracter, with 95% ethanol as extractant, solid-liquid ratio is 1: 10, extract
Temperature is 75 DEG C, and extraction time is 5 ~ 10min, and Fructus Litchi peel extract is obtained.
4)The preparation of antistaling agent:
Flos Lonicerae extract, Radix Glycyrrhizae extract, calcium chloride, glycerol, gibberellins and dehydroactic acid sodium are weighed by formula ratio,
Boiling water dissolves, shitosan needed for adding formula ratio while hot, stirs to being completely dissolved, is cooled to room temperature, and antistaling agent for litchi is obtained.
Six ~ medium well, epidermis not damaged, the litchi fruits of no disease and pests harm of fine day harvesting are selected, the present embodiment is soaked into
In obtained antistaling agent after 1 ~ 2min, natural air drying is placed in 4 ~ 6 DEG C of temperature and preserves, and rots by Fructus Litchi or browned area exceedes
It is set to rotten fruit during whole fruit area 1/4, after preservation 30 days, determines denaturation index, healthy fruit, rotting rate, solubility solid
The indexs such as thing, measurement result are as shown in table 4:
4 fresh-keeping effect synopsis of table(Cryopreservation 30d)
As a result, after showing Fructus Litchi sample cryopreservation 30d processed using the antistaling agent, the sense organ excellent flavor of Fructus Litchi is brown
Varying index is 1.66, and rotting rate is 13.3%, and healthy fruit reaches 86.7%, and sarcocarp soluble solid is 16.2 Brix, fresh-keeping effect
Fruit is substantially better than control fruit.
Above content is further description made for the present invention with reference to specific preferred implementation, it is impossible to assert
The present invention be embodied as be confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of without departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (8)
1. a kind of antistaling agent for litchi, it is characterised in that by weight percentage, its formula composition are as follows:
Flos Lonicerae extract 4 ~ 8%, Radix Glycyrrhizae extract 2 ~ 3%, Fructus Litchi peel extract 1 ~ 3%, calcium chloride 0.8 ~ 1.2%, glycerol 1.5
~ 2%, shitosan 0.2 ~ 0.6%, gibberellins 0.015 ~ 0.02%, dehydroactic acid sodium 0.8 ~ 1.2%, remaining are water.
2. a kind of method for preparing antistaling agent for litchi as claimed in claim 1, it is characterised in that including following step:
1)The preparation of Flos Lonicerae extract:
Flos Lonicerae is put into hyperpressure extraction tank, ethanol is added, pressurizes and keep pressure, pressure release to filter, filtrate is cold for the first time
Hide, the filtrate first time centrifugation after cold preservation, collect supernatant, supernatant system is collected in cold preservation again, second centrifugation
Obtain Flos Lonicerae extract;
2)The preparation of Radix Glycyrrhizae extract:
Weigh powder Radix Glycyrrhizae to be put in extraction kettle, be passed through CO2As fluid is extracted, supercritical is carried out as entrainer using ethanol
Extraction is obtained Radix Glycyrrhizae extract;
3)The preparation of Fructus Litchi peel extract:
Weigh powder litchi rind to be put in flash extracter, Fructus Litchi peel extract is obtained as extractant with 95% ethanol;
4)The preparation of antistaling agent:
Flos Lonicerae extract, Radix Glycyrrhizae extract, Fructus Litchi peel extract, calcium chloride, glycerol, gibberellins are weighed by formula ratio and is taken off
Hydroacetic acid sodium, boiling water dissolve, shitosan needed for adding formula ratio while hot, stir to being completely dissolved, are cooled to room temperature.
3. preparation method as claimed in claim 2, it is characterised in that the step 1)In, amount of alcohol added is Flos Lonicerae weight
5 ~ 8 times of amount.
4. preparation method as claimed in claim 2, it is characterised in that the step 1)In, moulding pressure is 100 ~ 400Mpa,
Pressure hold time is 10 ~ 15min.
5. preparation method as claimed in claim 2, it is characterised in that the step 1)In, first time refrigerated storage temperature is 1 ~ 4
DEG C, cold preservation time is 2 ~ 5h;Refrigerated storage temperature is 1-4 DEG C again, and cold preservation time is 1 ~ 3h.
6. preparation method as claimed in claim 2, it is characterised in that the step 1)In, the rotating speed of first time centrifugation
For 3000 ~ 3500r/min, disengaging time is 10 ~ 15min;The rotating speed of second centrifugation is 5000 ~ 6000r/min, separates
Time is 5 ~ 8min.
7. preparation method as claimed in claim 2, it is characterised in that the step 2)In, CO2Flow velocity is:15 ~ 18m/s, extraction
Pressure power is 30 ~ 35Mpa, and extraction temperature is 35 ~ 45 DEG C, and extraction time is 4 ~ 6h.
8. preparation method as claimed in claim 2, it is characterised in that the step 3)In, solid-liquid ratio is 1: 10 ~ 1: 15, carry
Temperature is taken for 70 ~ 80 DEG C, extraction time is 5 ~ 10min.
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CN1875727A (en) * | 2005-06-10 | 2006-12-13 | 许连科 | A method for preventing fresh lichee pericarp from darkening by citric acid |
CN101223908B (en) * | 2008-01-25 | 2010-11-17 | 中国农业大学 | Method for quick freezing litchi under low temperature |
CN101647488A (en) * | 2009-08-29 | 2010-02-17 | 茂名学院 | Lichi fresh-keeping agent |
CN102388957B (en) * | 2011-11-21 | 2013-06-12 | 广西壮族自治区农业科学院农产品加工研究所 | Lichee natural coating antistaling agent and its preparation method |
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