CN104459138A - Detection kit for insulin-like growth factor binding protein-1 - Google Patents
Detection kit for insulin-like growth factor binding protein-1 Download PDFInfo
- Publication number
- CN104459138A CN104459138A CN201410735829.6A CN201410735829A CN104459138A CN 104459138 A CN104459138 A CN 104459138A CN 201410735829 A CN201410735829 A CN 201410735829A CN 104459138 A CN104459138 A CN 104459138A
- Authority
- CN
- China
- Prior art keywords
- igfbp
- detection kit
- detection
- antibody
- damping fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
The invention relates to the field of bio-detection and particularly relates to a detection kit for quantitatively detecting an insulin-like growth factor binding protein-1, a preparation method and use thereof. The detection kit for the insulin-like growth factor binding protein-1 provided by the invention comprises a test paper card which comprises a bottom plate as well as a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are sequentially arranged from a sampling end on the surface of the bottom plate; the gold label pad comprises an insulin-like growth factor binding protein-1 antibody, a detection line and a quality control line are coated on the nitrocellulose membrane, and the insulin-like growth factor binding protein-1 antibody on the gold label pad is labeled by using a fluorescent microsphere. The kit provided by the invention can be used for detecting the insulin-like growth factor binding protein-1 by virtue of a fluorescent microsphere immunochromatography technology for the first time, has sensitivity and specificity and has the advantages of fast, simple and convenient operation, accurate result, economical benefit and practicality and the like.
Description
Technical field
The present invention relates to field of biological detection, particularly relate to a kind of IGFBP-1 detection kit and its production and use.
Background technology
IGFBP-1 (IGFBP-1) is the best approach for detecting rupture of membranes, is also the goldstandard through U.S. FDA approval.Countries in the world have the different appellations such as premature rupture of fetal membranes test strips, high phosphorylation, low phosphorylation and non-phosphorylating clinically, all refer to IGFBP-1 detection method.This method is better than other similar detection methods through clinical proof, dark blue IGFBP-1 detector bar accuracy high (being greater than 99%), is simple and easy to use.No matter in gynemetrics clinic, examination in hospital room, outpatient service or family, result equally accurately of all can attaining the Way.
Unphosphorylated IGFBP-1 mainly exists in amniotic fluid and is synthesized by decidual cell, and time just before giving birth, decidua is separated with chorion, and decidual cell fragment drains in Ovulation prediction.High 100 times in Volume fraction mother blood of IGFBP-1 in amniotic fluid ~ 1000 times, other body fluid are less, and Ovulation prediction, seminal fluid and urine are on testing all without impact.
IGFBP-1 in cervicovaginal secretions can be used as the objective indicator of Diagnosis of Premature Rupture.
Also there is more defect in IGFBP-1 detection method known at present, as comparatively loaded down with trivial details, consuming time longer in operation, is not suitable for using clinically.IGFBP-1 detection kit accuracy in front China market, sensitivity, repeatability are still poor.
Summary of the invention
The shortcoming of prior art in view of the above, the object of the present invention is to provide a kind of kit of Fast Measurement IGFBP-1 content, for solving the problems of the prior art.
For achieving the above object and other relevant objects, the invention provides a kind of kit of Fast Measurement IGFBP-1 content, comprise test card, described test card comprises base plate, and be positioned at the sample pad be arranged in order from application of sample end of backplate surface, gold mark pad, nitrocellulose filter and adsorptive pads, described gold mark pad comprises IGFBP-1 monoclonal antibody, described nitrocellulose filter is coated with detection line and nature controlling line, IGFBP-1 monoclonal antibody on described gold mark pad adopts fluorescent microsphere mark.
Preferably, IGFBP-1 monoclonal antibody on described gold mark pad adopts 180nm fluorescent microsphere mark, EX (nm)=650/Em (nm)=670, has signal little by background interference, detection sensitivity is high, the advantage that result is reproducible.
Preferably, described base plate is PVC base plate.
Preferably, on described nitrocellulose filter, detection line is positioned at side close to application of sample end, and nature controlling line is positioned at side away from application of sample end.
Preferably, described detection line is coated with IGFBP-1 monoclonal antibody.IGFBP-1 monoclonal antibody on described gold mark pad can be identical antibody with the IGFBP-1 monoclonal antibody on detection line, also can be different antibody.
Preferably, nature controlling line wraps by sheep anti-mouse antibody.
Preferably, described sample pad adopts damping fluid process, described damping fluid is selected from one or more the combination in PBS damping fluid, Tris-HCl damping fluid, glycine buffer, borate buffer solution and citrate-phosphate salt buffer, and the concentration of damping fluid is 20-200mM.
Preferably, gold mark of the present invention is paid somebody's debt and expected repayment later through pre-service, and the pre-treatment buffer used during pre-service is selected from one or more combination of glycine buffer, Tris-HCl damping fluid, borate buffer solution, and the concentration of damping fluid is 5-50mM.
Preferably, described damping fluid also comprises increased response agent, described increased response agent be selected from PEG4000, PEG6000, PEG8000 and PEG20000 any one, the concentration of described increased response agent is 10 ~ 50g/L.
Preferably, described buffer solution also comprises surfactant, described surfactant is selected from any one or multiple combination in S-19TWEEN 20, S-20TWEEN 80, S-13TRITON X-45, S-14TRITON X-100, S-15TRITON X305, and the concentration of described surfactant is 10 ~ 50g/L.
Preferably, in order to make kit, there is better sensitivity and color developing effect, gold mark of the present invention pads the pre-treatment buffer used when pre-service and comprises following component: stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate and glycocoll, and the total concentration of stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate, glycocoll is 3.5-7.5g/L, the pH value of damping fluid is 7.2-7.6.
Preferably, the concentration of each component in damping fluid is:
The solvent of described pre-treatment buffer is water.
Described pretreated concrete steps are: gold mark pad is soaked 1.5 ~ 2h in pretreatment fluid, take out and are put in 36 ~ 38 DEG C of oven dry.
Described pre-treatment buffer can use the various conventional pH adjusting agent in this area to carry out the adjustment of pH value.
Preferably, described kit also comprises and getting stuck, described getting stuck comprises back of the body card and upper cover, the described back of the body is arranged with test card draw-in groove, described test card is embedded in described test card draw-in groove, be covered with testing window and well on described, matching with the position of described detection line and nature controlling line in the position of described testing window, matches with the position of described sample pad in the position of described well.
Preferred, described in get stuck for plastics get stuck.
Preferably, described detection kit is used for the content quantitatively detecting IGFBP-1.
The detection kit of quantitative detection IGFBP-1 provided by the present invention, can supporting immune quantitative analytical instrument use.Immunoassay instrument, by acquisition testing line (T) and nature controlling line (C) band fluorescence signal, calculates T/C signal value.First various criterion product are added drop-wise on test card before using, analyzing and processing sets up calibration curve (relation of T/C signal value and standard items actual value), again the T/C value obtained when detecting sample is compared with typical curve, the IGFBP-1 content detected in sample can be obtained.
Second aspect present invention provides the preparation method of the detection kit of described quantitative detection lipoprotein IGFBP-1, comprises the steps:
1) with the IGFBP-1 antibody-solutions spraying gold mark pad of fluorescent microsphere mark, the obtained gold mark pad comprising IGFBP-1 antibody;
2) on the detection line and nature controlling line of nitrocellulose filter, spray IGFBP-1 antibody and sheep anti-mouse antibody respectively, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) gold mark pad, the step 2 prepared) nitrocellulose filter, the adsorptive pads prepared be pasted onto on base plate successively, and cutting obtains Test paper card; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
Preferably, gold mark of the present invention is paid somebody's debt and expected repayment later through pre-service, and the pre-treatment buffer used during pre-service is selected from one or more combination of glycine buffer, Tris-HCl damping fluid, borate buffer solution, and the concentration of damping fluid is 5-50mM.
Preferably, in order to make kit, there is better sensitivity and color developing effect, gold mark of the present invention pads the pre-treatment buffer used when pre-service and comprises following component: stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate and glycocoll, and the total concentration of stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate, glycocoll is 3.5-7.5g/L, the pH value of damping fluid is 7.2-7.6.
Preferably, the concentration of each component in damping fluid is:
The solvent of described pre-treatment buffer is water.
Described pretreated concrete steps are: gold mark pad is soaked 1.5 ~ 2h in pretreatment fluid, take out and are put in 36 ~ 38 DEG C of oven dry.
Described pre-treatment buffer can use the various conventional pH adjusting agent in this area to carry out the adjustment of pH value.
Third aspect present invention provides the purposes of detection kit at IGFBP-1 detection field of described quantitative detection lipoprotein IGFBP-1.
Beneficial effect of the present invention is:
IGFBP-1 is detected by fluorescent micro-ball immune chromatography technology by the detection kit of quantitative detection IGFBP-1 provided by the present invention first, have high sensitivity and high specific concurrently, the content of IGFBP-1 can be detected fast.In addition, described detection kit has the advantages such as operation is fast and convenient, result is accurate, economic and practical, disturb little by the serious blood fat of serum (or blood plasma), haemolysis, as serum (or blood plasma) haemoglobin≤500mg/L, triglyceride≤30mg/dL, variation < 10% is affected on accuracy.
Embodiment
Below by way of specific instantiation, embodiments of the present invention are described, those skilled in the art the content disclosed by this instructions can understand other advantages of the present invention and effect easily.The present invention can also be implemented or be applied by embodiments different in addition, and the every details in this instructions also can based on different viewpoints and application, carries out various modification or change not deviating under spirit of the present invention.
Before further describing the specific embodiment of the invention, should be understood that protection scope of the present invention is not limited to following specific specific embodiments; It is also understood that the term used in the embodiment of the present invention is to describe specific specific embodiments, instead of in order to limit the scope of the invention; In instructions of the present invention and claims, unless explicitly pointed out in addition in literary composition, singulative " ", " one " and " this " comprise plural form.
When embodiment provides numerical range, should be understood that except non-invention is otherwise noted, between two end points of each numerical range and two end points, any one numerical value all can be selected.Unless otherwise defined, all technology used in the present invention are identical with the meaning that those skilled in the art of the present technique understand usually with scientific terminology.Except the concrete grammar used in embodiment, equipment, material, according to those skilled in the art to the grasp of prior art and record of the present invention, any method of prior art that is similar with the method described in the embodiment of the present invention, equipment, material or that be equal to, equipment and material can also be used to realize the present invention.
Unless otherwise indicated, disclosed in the present invention experimental technique, detection method, preparation method all adopt the routine techniques of the molecular biology of the art routine, biological chemistry, chromatin Structure and analysis, analytical chemistry, cell chulture, recombinant DNA technology and association area.These technology are existing in existing document improves explanation, specifically can see the MOLECULAR CLONING:A LABORATORY MANUAL such as Sambrook, Second edition, Cold Spring HarborLaboratory Press, 1989and Third edition, 2001; Ausubel etc., CURRENT PROTOCOLS INMOLECULAR BIOLOGY, John Wiley & Sons, New York, 1987and periodic updates; The seriesMETHODS IN ENZYMOLOGY, Academic Press, San Diego; Wolffe, CHROMATINSTRUCTURE AND FUNCTION, Third edition, Academic Press, San Diego, 1998; METHODSIN ENZYMOLOGY, Vol.304, Chromatin (P.M.Wassarman and A.P.Wolffe, eds.), AcademicPress, San Diego, 1999; With METHODS IN MOLECULAR BIOLOGY, Vol.119, ChromatinProtocols (P.B.Becker, ed.) Humana Press, Totowa, 1999 etc.
Embodiment 1
The preparation of test card of the present invention:
1) pre-treatment buffer is used to carry out pre-service to gold mark pad, pre-treatment buffer is: stachyose 2g/L, alum 0.5g/L, Fructose Diphosphate 1.5g/L, sodium hexametaphosphate 0.3g/L, the aqueous solution of glycocoll 1.88g/L, pH=7.4, pretreated concrete steps are: gold mark pad is soaked 2h in pretreatment fluid, take out and are put in 37 DEG C of oven dry; Then with the IGFBP-1 antibody-solutions spraying pretreated gold mark pad of fluorescent microsphere mark, obtained bag is by the gold mark pad of IGFBP-1 antibody, in solution, the mass ratio of fluorescent microsphere and antibody is 5:1, the concentration of solution is 10mg/ml, and quantity for spray is 4ul/cm;
2) on the detection line and nature controlling line of nitrocellulose filter, spray IGFBP-1 antibody-solutions and the sheep anti-mouse antibody solution of 1mg/ml respectively, quantity for spray is 1ul/cm, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter, the adsorptive pads prepared be pasted onto on PVC base plate successively, cutting obtains the Test paper card of wide 3-5mm; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
Standard lines curve:
Be 0 by concentration respectively, 5, 20, 50, 100, 200, 400, 600, 800, the IGFBP-1 buffer solution of 1000pg/ml drips in sample pad, each concentration establishes 5 repetitions (testing result gets 5 mean values repeated), after rete is analysed 10 minutes, use immunoassay instrument by acquisition testing line (T) and nature controlling line (C) band fluorescence signal, the sensing range of analyser to fluorescence signal is AD value 0-10000, calculate T/C signal value, set up calibration curve, wherein Y-axis is T/C signal value, X-axis is standard items actual value.
IGFBP-1 anti-interference and specific detection:
Detection sample drop is added in sample pad, 5 repetitions (testing result gets 5 mean values repeated) established by each sample, after rete is analysed 10 minutes, the T/C value obtained when detecting sample is compared with typical curve, obtain the detection data of the IGFBP-1 content detected in sample, the IGFBP-1 content data and true IGFBP-1 content data that detect acquisition are contrasted, obtaining accuracy affects deviate again.
Sample 1:10pg/ml IGFBP-1,50mg/L haemoglobin, 50mg/dL triglyceride;
Sample 2:60pg/ml IGFBP-1,500mg/L haemoglobin, 10mg/dL triglyceride;
Sample 3:120pg/ml IGFBP-1,100mg/L haemoglobin, 20mg/dL triglyceride;
Sample 4:250pg/ml IGFBP-1,150mg/L haemoglobin, 30mg/dL triglyceride;
Sample 5:1000pg/ml IGFBP-1,200mg/L haemoglobin, 40mg/dL triglyceride;
Blank: 50mg/L haemoglobin, 50mg/dL triglyceride;
The IGFBP-1 content data of the detection that sample 1-5 obtains is respectively 10.01pg/ml, 59.99pg/ml, 120.02pg/ml, 249.5pg/ml, 1000.01pg/ml, accuracy affect variation < 10%, do not find when detecting in blank that obvious fluorescence signal changes.
Embodiment 2
The preparation of comparative example test card:
Adopt 25mM glycine buffer pre-service gold mark pad, other reagent and experimental technique are all with embodiment 1.
1) 25mM glycine buffer pre-service gold mark pad is adopted, then with the IGFBP-1 antibody-solutions spraying pretreated gold mark pad of fluorescent microsphere mark, obtained bag is by the gold mark pad of IGFBP-1 antibody, in solution, the mass ratio of fluorescent microsphere and antibody is 5:1, the concentration of solution is 10mg/ml, and quantity for spray is 4ul/cm;
2) on the detection line and nature controlling line of nitrocellulose filter, spray IGFBP-1 antibody-solutions and the sheep anti-mouse antibody solution of 1mg/ml respectively, quantity for spray is 1ul/cm, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter, the adsorptive pads prepared be pasted onto on PVC base plate successively, cutting obtains the Test paper card of wide 3-5mm; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
The sensitivity of IGFBP-1 and detectability contrast experiment:
Judge with fluorescent instrument, analyser be AD value 0-10000 to the sensing range of fluorescence signal, according to the performance of instrument, CUTOFF value is 50, under certain concentration, more than 90% test example AD value >=50, namely think that kit can be used in the detection under this concentration.
Adopt 5%BSA normal saline solution as dummy, dummy should not contain measured object.The IGFBP-1 known sample getting 1 ~ 100pg/mL gradient concentration carries out sensitivity detection, arranges a gradient at interval of 2pg/mL, and each gradient arranges 50 samples, record testing result.The lowest detection that result shows the kit prepared by embodiment 1 is limited to 1.00pg/mL; And the lowest detectable limit of the kit prepared by embodiment 2 is higher than 100pg/mL.
In sum, detection kit provided by the present invention has good anti-interference and specificity, and has good sensitivity, and negative background is lower, effectively overcomes various shortcoming of the prior art and tool high industrial utilization.
Above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art scholar all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as have in art usually know the knowledgeable do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.
Claims (10)
1. one kind is quantitatively detected the detection kit of IGFBP-1, comprise test card, test card comprises base plate and is positioned at the sample pad be arranged in order from application of sample end of backplate surface, gold mark pad, nitrocellulose filter and adsorptive pads, described gold mark pad comprises IGFBP-1 antibody, described nitrocellulose filter is coated with detection line and nature controlling line, the IGFBP-1 antibody on described gold mark pad adopts fluorescent microsphere mark.
2. the detection kit quantitatively detecting IGFBP-1 as claimed in claim 1, it is characterized in that, on described nitrocellulose filter, detection line is positioned at side close to application of sample end, and nature controlling line is positioned at side away from application of sample end.
3. the detection kit quantitatively detecting IGFBP-1 as claimed in claim 1, is characterized in that, described detection line is coated with IGFBP-1 antibody.
4. the detection kit quantitatively detecting IGFBP-1 as claimed in claim 1, is characterized in that, nature controlling line wraps by sheep anti-mouse antibody.
5. the detection kit quantitatively detecting IGFBP-1 as claimed in claim 1, it is characterized in that, described sample pad adopts damping fluid process, described damping fluid is selected from one or more the combination in PBS damping fluid, Tris-HCl damping fluid, glycine buffer, borate buffer solution and citrate-phosphate salt buffer, and the concentration of damping fluid is 20-200mM.
6. the detection kit quantitatively detecting IGFBP-1 as claimed in claim 1, it is characterized in that, described gold mark pad adopts damping fluid process, described damping fluid is selected from one or more combination of glycine buffer, Tris-HCl damping fluid, borate buffer solution, and the concentration of damping fluid is 5-50mM.
7. the detection kit quantitatively detecting IGFBP-1 as claimed in claim 6, it is characterized in that, increased response agent is also comprised in described damping fluid, described increased response agent be selected from PEG4000, PEG6000, PEG8000 or PEG20000 any one, the concentration of described increased response agent is 10 ~ 50g/L.
8. the detection kit quantitatively detecting IGFBP-1 as claimed in claim 1, it is characterized in that, also comprise and getting stuck, described getting stuck comprises back of the body card and upper cover, the described back of the body is arranged with test card draw-in groove, and described test card is embedded in described test card draw-in groove, is covered with testing window and well on described, matching with the position of described detection line and nature controlling line in the position of described testing window, matches with the position of described sample pad in the position of described well.
9. the detection kit quantitatively detecting fat IGFBP-1 as claimed in claim 1, is characterized in that, described detection kit is used for the content quantitatively detecting IGFBP-1.
10. the preparation method of the detection kit of the quantitative detection fat IGFBP-1 according to the arbitrary claim of claim 1 ~ 9, specifically comprises the steps:
1) with the IGFBP-1 antibody-solutions spraying gold mark pad of fluorescent microsphere mark, the obtained gold mark pad comprising IGFBP-1 antibody;
2) on the detection line and nature controlling line of nitrocellulose filter, spray IGFBP-1 antibody and sheep anti-mouse antibody respectively, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) gold mark pad, the step 2 prepared) nitrocellulose filter, the adsorptive pads prepared be pasted onto on base plate successively, and cutting obtains Test paper card; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410735829.6A CN104459138B (en) | 2014-12-05 | 2014-12-05 | A kind of IGFBP-1 detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410735829.6A CN104459138B (en) | 2014-12-05 | 2014-12-05 | A kind of IGFBP-1 detection kit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104459138A true CN104459138A (en) | 2015-03-25 |
| CN104459138B CN104459138B (en) | 2016-03-09 |
Family
ID=52905524
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410735829.6A Active CN104459138B (en) | 2014-12-05 | 2014-12-05 | A kind of IGFBP-1 detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104459138B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242051A (en) * | 2015-09-23 | 2016-01-13 | 上海凯璟生物科技有限公司 | Up-conversion luminescence-based human insulin-like growth factor binding protein-1 (IGFBP-1) chip detection system and reagent |
| CN106053444A (en) * | 2016-04-28 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | A kit for measuring a specific growth factor and a preparing method thereof |
| CN109521207A (en) * | 2018-12-28 | 2019-03-26 | 广州菲康生物技术有限公司 | A kind of IGF-1 fluorescence immune chromatography detection kit |
| CN110618266A (en) * | 2019-09-26 | 2019-12-27 | 河北省科学院生物研究所 | Kit for detecting mastitis of dairy cow and use method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004110308A2 (en) * | 2003-06-11 | 2004-12-23 | Osteotech, Inc. | Osteoimplants and methods for their manufacture |
| WO2010119295A1 (en) * | 2009-04-16 | 2010-10-21 | Cambridge Enterprise Limited | Biomarkers |
| CN102053157A (en) * | 2010-11-19 | 2011-05-11 | 广州万孚生物技术有限公司 | Test strip for fast detecting premature rupture of fetal membranes |
| CN103926401A (en) * | 2014-03-31 | 2014-07-16 | 瑞莱生物科技（江苏）有限公司 | Immunofluorescence test paper strip for rapidly and quantitatively measuring IGFBP-7 and TIMP-2 and preparation method thereof |
-
2014
- 2014-12-05 CN CN201410735829.6A patent/CN104459138B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004110308A2 (en) * | 2003-06-11 | 2004-12-23 | Osteotech, Inc. | Osteoimplants and methods for their manufacture |
| WO2010119295A1 (en) * | 2009-04-16 | 2010-10-21 | Cambridge Enterprise Limited | Biomarkers |
| CN102053157A (en) * | 2010-11-19 | 2011-05-11 | 广州万孚生物技术有限公司 | Test strip for fast detecting premature rupture of fetal membranes |
| CN103926401A (en) * | 2014-03-31 | 2014-07-16 | 瑞莱生物科技（江苏）有限公司 | Immunofluorescence test paper strip for rapidly and quantitatively measuring IGFBP-7 and TIMP-2 and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242051A (en) * | 2015-09-23 | 2016-01-13 | 上海凯璟生物科技有限公司 | Up-conversion luminescence-based human insulin-like growth factor binding protein-1 (IGFBP-1) chip detection system and reagent |
| CN106053444A (en) * | 2016-04-28 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | A kit for measuring a specific growth factor and a preparing method thereof |
| CN109521207A (en) * | 2018-12-28 | 2019-03-26 | 广州菲康生物技术有限公司 | A kind of IGF-1 fluorescence immune chromatography detection kit |
| CN110618266A (en) * | 2019-09-26 | 2019-12-27 | 河北省科学院生物研究所 | Kit for detecting mastitis of dairy cow and use method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104459138B (en) | 2016-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104360085B (en) | A kind of AFP detection kit | |
| Pedersen et al. | Quantification of multiple elements in dried blood spot samples | |
| CN104459138B (en) | A kind of IGFBP-1 detection kit | |
| Chen et al. | Determination of bisphenol‐A levels in human amniotic fluid samples by liquid chromatography coupled with mass spectrometry | |
| KR20150119118A (en) | Means and methods for assessing the quality of a biological sample | |
| CN105548547A (en) | Flow type array immunoassay kit for detecting lung cancer markers based on flow cytometry | |
| Pokhrel et al. | Selection of appropriate protein assay method for a paper microfluidics platform | |
| Hogeling et al. | Quantification of proteins in whole blood, plasma and DBS, with element-labelled antibody detection by ICP-MS | |
| CN104459140A (en) | Detection kit for quantitative detection of rheumatoid factor, antistreptolysin O, anti cyclic citrullinated peptide antibody and C reactive protein | |
| CN104360071A (en) | Kit for quickly determining content of glycocholic acid (CG) in serum or blood plasma | |
| CN104391121B (en) | A kind of fetal fibronectin detection kit | |
| CN104360083B (en) | A kind of detection by quantitative rheumatoid factor, antistreptolysin O (ASO), the detection kit of c reactive protein | |
| CN104374911A (en) | Detection kit for quantitative detection of rheumatoid factors and anti streptolysin O | |
| Albu et al. | Validated HPLC-Fl method for the analysis of S-adenosylmethionine and S-adenosylhomocysteine biomarkers in human blood | |
| CN104360080B (en) | A kind of golgiosome glycoprotein-73 detection kit | |
| CN108120714A (en) | A kind of reagent for detecting urine microdose urine protein and its application | |
| CN104391122B (en) | A kind of AFP, PIVKA-II combined detection kit | |
| CN109613127A (en) | A kind of method and detection kit for identifying urarthritis biomarker | |
| Evin et al. | LX-8000R and UriSed 2 fully automated urine analyzers comparison to manual microscopic examination | |
| CN104407155B (en) | A kind of AFP, GP73, PIVKA-II combined detection kit | |
| CN104374927B (en) | A kind of detection kit of quantitative detection LP(a) | |
| US20140011190A1 (en) | Method for performing a rapid test | |
| CN103616522A (en) | Immune chromatography result identification method based on envelope area and twice compensation | |
| CN104360082B (en) | A kind of AFP, GP73 combined detection kit | |
| CN104360079A (en) | PIVKA-II detection kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171024 Address after: 400000 Taikang Road Dadukou District of Chongqing Bridge Industrial Park C District No. 6 Building 30 layer 1-4 Patentee after: Chongqing Zhongyuan Ji Ji Biological Technology Co., Ltd. Address before: Jiulongpo Branch Park four street 400039 Chongqing city 70-1, 70-2 block J No. six Patentee before: CHONGQING QIANDE BIOTECHNOLOGY CO., LTD. |
|
| TR01 | Transfer of patent right | ||
| CP03 | Change of name, title or address |
Address after: Floor 1-4, building 30, No. 6, Taikang Road, Zone C, Jianqiao Industrial Park, Dadukou District, Chongqing 404100 Patentee after: Zhongyuan Huiji Biotechnology Co.,Ltd. Address before: Floor 1-4, building 30, no.6, Taikang Road, Zone C, Jianqiao Industrial Park, Dadukou District, Chongqing 400000 Patentee before: CHONGQING ZHONGYUAN HUIJI BIOTECHNOLOGY Co.,Ltd. |
|
| CP03 | Change of name, title or address |