CN104360071A - Kit for quickly determining content of glycocholic acid (CG) in serum or blood plasma - Google Patents
Kit for quickly determining content of glycocholic acid (CG) in serum or blood plasma Download PDFInfo
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- CN104360071A CN104360071A CN201410736270.9A CN201410736270A CN104360071A CN 104360071 A CN104360071 A CN 104360071A CN 201410736270 A CN201410736270 A CN 201410736270A CN 104360071 A CN104360071 A CN 104360071A
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- glycocholic acid
- detection kit
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Abstract
The invention relates to the field of biological detection and in particular relates to a detection kit used for quantitatively detecting glycocholic acid (CG) as well as a preparation method and application of the detection kit. The detection kit used for quantitatively detecting CG comprises a test paper card, wherein the test paper card comprises a base plate as well as a sample pad, a gold mark pad, a nitrocellulose membrane and a water absorption pad which are sequentially arranged from a sampling end positioned on the surface of the base plate, the gold mark pad contains a CG antibody, the nitrocellulose membrane is coated with a detection line and a quality control line, and the CG antibody on the gold mark pad is marked by adopting a fluorescent microsphere. The detection kit provided by the invention can be used for detecting CG by adopting a fluorescent microsphere immunochromatographic technology initially, has sensitivity and specificity and has the advantages of quickness and simplicity in operation, accurate result, economic applicability and the like.
Description
Technical field
The present invention relates to field of biological detection, particularly relate to kit of content of glycocholic acid and its production and use in a kind of Fast Measurement serum or blood plasma.
Background technology
Glycocholic acid (Cholyglycine, CG) is the mating type cholic acid that cholic acid and glycocoll are combined into, and in liver cell, the enzymatic reaction of cholesterol process and complexity thereof, is transformed into primary bile acid.Wherein there are cholic acid (CA) and chenodeoxycholic acid (CD-CA).The steroids core of cholic acid has three hydroxyls (C3, C7, C12), the hydroxyl of side chain terminal is combined with glycocoll with peptide bond.
CG is synthesized by liver cell, and eubolism approach is the circulation of intestines liver, enters gall-bladder through bile capillaries, bile duct, enters duodenum in company with bile, helps food digestion.95% cholic acid heavily absorbs at terminal ileum, and trans-portal vein returns liver again, absorbs recycling by liver cell.Mainly exist with protein-bound form in serum, the total amount overflowing into body circulation is less than 1%.Under normal circumstances, in peripheral blood, cholic acid content is very micro-, and adult normal no matter on an empty stomach or after the meal, its change of serum C G concentration stabilize is in low-level.When liver cell is impaired, liver cell picked-up CG ability declines, and causes CG content in blood to increase; During cholestegnosis, hepatic excretion cholic acid generation obstacle, and the sanguimotor CG content that backflows increases, and also makes blood CG content increase.Measuring Serum CG (CG) is one of sensitive indicator evaluating hepatocyte function and liver and gall system material recycle function thereof.
Oxyhepatitis, chronic active hepatitis, primary carcinoma of liver, cirrhosis, chronic persistent hepatitis, obstructive liver disease, intestines-hepatic circulatory disturbance, cholelithiasis companion jaundice, bile duct, gall-bladder excretory function impaired patients blood CG are all apparently higher than normal person clinically.
Glycocholic acid is also topmost bile acid component in serum third trimester of pregnancy simultaneously.During normal pregnancy, Serum CG Levels in Pregnant Women progressively increased with pregnant week, and a large amount of estrogen can cause the change of liver plasma membrane, and reduce the mobility of hole shape film slurry, thus occur that choleresis reduces, backflow into blood, occur cholestasis phenomenon in the gestational period, CG level raises.All be in inside and outside placental villi venous lumen among the stimulation of high concentration bile acid, cause vessel retraction, placental villi vascular surface spasm, oxygenated blood flow reduces, and causes fetal anoxia, can occur various complication, as fetal distress in uterus, premature labor etc.
Glycocholic acid detection method known at present has radioimmunology (RIA), enzyme linked immunosorbent assay (ELISA), chemoluminescence method (CL) etc.But these methods all exist more defect, as radioimmunology isotope reagent has the drawback such as radioactive contamination and the shorter and inconvenient operation of the term of validity, enzyme linked immunosorbent assay operation is comparatively loaded down with trivial details, consuming time longer, is not suitable for using clinically.Although chemoluminescence method sensitivity is higher, need special sensing equipment, be costly unfavorable for promoting.And latex enhancing immune turbidimetry easy and simple to handle, be applicable to automatic clinical chemistry analyzer, detection time was less than 10 minutes applicable clinical expansions.But the glycocholic acid detection kit accuracy at present in China market, sensitivity, repeatability are still poor.
Summary of the invention
The shortcoming of prior art in view of the above, the object of the present invention is to provide the kit of glycocholic acid (CG) content in a kind of Fast Measurement serum or blood plasma, for solving the problems of the prior art.
For achieving the above object and other relevant objects, the invention provides the kit of glycocholic acid (CG) content in a kind of Fast Measurement serum or blood plasma, comprise test card, described test card comprises base plate and is positioned at the sample pad be arranged in order from application of sample end of backplate surface, gold mark pad, nitrocellulose filter and adsorptive pads, described gold mark pad comprises CG monoclonal antibody, described nitrocellulose filter is coated with detection line and nature controlling line, the CG monoclonal antibody on described gold mark pad adopts fluorescent microsphere mark.
Preferably, the CG monoclonal antibody on described gold mark pad adopts 180nm fluorescent microsphere mark, EX (nm)=650/Em (nm)=670, and have signal little by background interference, detection sensitivity is high, the advantage that result is reproducible.
Preferably, described base plate is PVC base plate.
Preferably, on described nitrocellulose filter, detection line is positioned at side close to application of sample end, and nature controlling line is positioned at side away from application of sample end.
Preferably, described detection line is coated with CG monoclonal antibody.CG monoclonal antibody on described gold mark pad can be identical antibody with the CG monoclonal antibody on detection line, also can be different antibody.
Preferably, nature controlling line wraps by sheep anti-mouse antibody.
Preferably, described sample pad adopts damping fluid process, described damping fluid is selected from one or more the combination in PBS damping fluid, Tris-HCl damping fluid, glycine buffer, borate buffer solution and citrate-phosphate salt buffer, and the concentration of damping fluid is 20-200mM.
Preferably, gold mark of the present invention is paid somebody's debt and expected repayment later through pre-service, and the pre-treatment buffer used during pre-service is selected from one or more combination of glycine buffer, Tris-HCl damping fluid, borate buffer solution, and the concentration of damping fluid is 5-50mM.
Preferably, described damping fluid also comprises increased response agent, described increased response agent be selected from PEG4000, PEG6000, PEG8000 and PEG20000 any one, the concentration of described increased response agent is 10 ~ 50g/L.
Preferably, described buffer solution also comprises surfactant, described surfactant is selected from any one or multiple combination in S-19 TWEEN 20, S-20TWEEN 80, S-13 TRITON X-45, S-14 TRITON X-100, S-15 TRITON X305, and the concentration of described surfactant is 10 ~ 50g/L.
Preferably, in order to make kit, there is better sensitivity and color developing effect, gold mark of the present invention pads the pre-treatment buffer used when pre-service and comprises following component: stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate and glycocoll, and the total concentration of stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate, glycocoll is 3.5-7.5g/L, the pH value of damping fluid is 7.2-7.6.
Preferably, the concentration of each component in damping fluid is:
The solvent of described pre-treatment buffer is water.
Described pretreated concrete steps are: gold mark pad is soaked 1.5 ~ 2h in pretreatment fluid, take out and are put in 36 ~ 38 DEG C of oven dry.
Described pre-treatment buffer can use the various conventional pH adjusting agent in this area to carry out the adjustment of pH value.
Preferably, described kit also comprises and getting stuck, described getting stuck comprises back of the body card and upper cover, the described back of the body is arranged with test card draw-in groove, described test card is embedded in described test card draw-in groove, be covered with testing window and well on described, matching with the position of described detection line and nature controlling line in the position of described testing window, matches with the position of described sample pad in the position of described well.
Preferred, described in get stuck for plastics get stuck.
Preferably, described detection kit is used for the content quantitatively detecting glycocholic acid (CG) in serum or blood plasma.
The detection kit of quantitative detection glycocholic acid (CG) provided by the present invention adopts Competitive assays immunochromatographic method when detecting glycocholic acid (CG), and supporting immune quantitative analytical instrument uses.Immunoassay instrument, by acquisition testing line (T) and nature controlling line (C) band fluorescence signal, calculates T/C signal value.First various criterion product are added drop-wise on test card before using, analyzing and processing sets up calibration curve (relation of T/C signal value and standard items actual value), again the T/C value obtained when detecting sample is compared with typical curve, glycocholic acid (CG) content detected in sample can be obtained.
Second aspect present invention provides the preparation method of the detection kit of described quantitative detection lipoprotein glycocholic acid (CG), comprises the steps:
1) with glycocholic acid (CG) the antibody-solutions spraying gold mark pad of fluorescent microsphere mark, the obtained gold mark pad comprising glycocholic acid (CG) antibody;
2) on the detection line and nature controlling line of nitrocellulose filter, spray glycocholic acid (CG) antibody and sheep anti-mouse antibody respectively, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter, the adsorptive pads prepared be pasted onto on base plate successively, cutting obtains Test paper card; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
Preferably, gold mark of the present invention is paid somebody's debt and expected repayment later through pre-service, and the pre-treatment buffer used during pre-service is selected from one or more combination of glycine buffer, Tris-HCl damping fluid, borate buffer solution, and the concentration of damping fluid is 5-50mM.
Preferably, in order to make kit, there is better sensitivity and color developing effect, gold mark of the present invention pads the pre-treatment buffer used when pre-service and comprises following component: stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate and glycocoll, and the total concentration of stachyose, alum, Fructose Diphosphate, sodium hexametaphosphate, glycocoll is 3.5-7.5g/L, the pH value of damping fluid is 7.2-7.6.
Preferably, the concentration of each component in damping fluid is:
The solvent of described pre-treatment buffer is water.
Described pretreated concrete steps are: gold mark pad is soaked 1.5 ~ 2h in pretreatment fluid, take out and are put in 36 ~ 38 DEG C of oven dry.
Described pre-treatment buffer can use the various conventional pH adjusting agent in this area to carry out the adjustment of pH value.
Third aspect present invention provides the purposes of detection kit at glycocholic acid (CG) detection field of described quantitative detection lipoprotein glycocholic acid (CG).
Beneficial effect of the present invention is:
Glycocholic acid (CG) is detected by fluorescent micro-ball immune chromatography technology by the detection kit of quantitative detection glycocholic acid (CG) provided by the present invention first, have high sensitivity and high specific concurrently, the content of glycocholic acid (CG) can be detected fast.In addition, described detection kit has the advantages such as operation is fast and convenient, result is accurate, economic and practical, disturb little by the serious blood fat of serum (or blood plasma), haemolysis, as serum (or blood plasma) haemoglobin≤500mg/L, triglyceride≤30mg/dL, variation < 10% is affected on accuracy.
Embodiment
Below by way of specific instantiation, embodiments of the present invention are described, those skilled in the art the content disclosed by this instructions can understand other advantages of the present invention and effect easily.The present invention can also be implemented or be applied by embodiments different in addition, and the every details in this instructions also can based on different viewpoints and application, carries out various modification or change not deviating under spirit of the present invention.
Before further describing the specific embodiment of the invention, should be understood that protection scope of the present invention is not limited to following specific specific embodiments; It is also understood that the term used in the embodiment of the present invention is to describe specific specific embodiments, instead of in order to limit the scope of the invention; In instructions of the present invention and claims, unless explicitly pointed out in addition in literary composition, singulative " ", " one " and " this " comprise plural form.
When embodiment provides numerical range, should be understood that except non-invention is otherwise noted, between two end points of each numerical range and two end points, any one numerical value all can be selected.Unless otherwise defined, all technology used in the present invention are identical with the meaning that those skilled in the art of the present technique understand usually with scientific terminology.Except the concrete grammar used in embodiment, equipment, material, according to those skilled in the art to the grasp of prior art and record of the present invention, any method of prior art that is similar with the method described in the embodiment of the present invention, equipment, material or that be equal to, equipment and material can also be used to realize the present invention.
Unless otherwise indicated, disclosed in the present invention experimental technique, detection method, preparation method all adopt the routine techniques of the molecular biology of the art routine, biological chemistry, chromatin Structure and analysis, analytical chemistry, cell chulture, recombinant DNA technology and association area.These technology are existing in existing document improves explanation, specifically can see the MOLECULAR CLONING:A LABORATORY MANUAL such as Sambrook, Second edition, Cold Spring HarborLaboratory Press, 1989and Third edition, 2001; Ausubel etc., CURRENT PROTOCOLS INMOLECULAR BIOLOGY, John Wiley & Sons, New York, 1987and periodic updates; The seriesMETHODS IN ENZYMOLOGY, Academic Press, San Diego; Wolffe, CHROMATINSTRUCTURE AND FUNCTION, Third edition, Academic Press, San Diego, 1998; METHODSIN ENZYMOLOGY, Vol.304, Chromatin (P.M.Wassarman and A.P.Wolffe, eds.), AcademicPress, San Diego, 1999; With METHODS IN MOLECULAR BIOLOGY, Vol.119, ChromatinProtocols (P.B.Becker, ed.) Humana Press, Totowa, 1999 etc.
Embodiment 1
The preparation of test card of the present invention:
1) pre-treatment buffer is used to carry out pre-service to gold mark pad, pre-treatment buffer is: stachyose 2g/L, alum 0.5g/L, Fructose Diphosphate 1.5g/L, sodium hexametaphosphate 0.3g/L, the aqueous solution of glycocoll 1.88g/L, pH=7.4, pretreated concrete steps are: gold mark pad is soaked 2h in pretreatment fluid, take out and are put in 37 DEG C of oven dry; Then with glycocholic acid (CG) the antibody-solutions spraying pretreated gold mark pad of fluorescent microsphere mark, obtained bag is by the gold mark pad of glycocholic acid (CG) antibody, in solution, the mass ratio of fluorescent microsphere and antibody is 5:1, and the concentration of solution is 10mg/ml, and quantity for spray is 4ul/cm;
2) on the detection line and nature controlling line of nitrocellulose filter, spray glycocholic acid (CG) antibody-solutions and the sheep anti-mouse antibody solution of 1mg/ml respectively, quantity for spray is 1ul/cm, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter, the adsorptive pads prepared be pasted onto on PVC base plate successively, cutting obtains the Test paper card of wide 3-5mm; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
Standard lines curve:
Be 0 respectively by concentration, 20,60,100,150,200,250,300,400, glycocholic acid (CG) buffer solution of 500mg/L drips in sample pad, each concentration establishes 5 repetitions (testing result gets 5 mean values repeated), after rete is analysed 10 minutes, use immunoassay instrument by acquisition testing line (T) and nature controlling line (C) band fluorescence signal, the sensing range of analyser to fluorescence signal is AD value 0-10000, calculate T/C signal value, set up calibration curve, wherein Y-axis is T/C signal value, and X-axis is standard items actual value.
Glycocholic acid (CG) anti-interference and specific detection:
Detection sample drop is added in sample pad, 5 repetitions (testing result gets 5 mean values repeated) established by each sample, after rete is analysed 10 minutes, the T/C value obtained when detecting sample is compared with typical curve, obtain the detection data of glycocholic acid (CG) content detected in sample, glycocholic acid (CG) content data and true glycocholic acid (CG) content data that detect acquisition are contrasted, obtaining accuracy affects deviate again.
Blood serum sample 1:25mg/L glycocholic acid (CG), 50mg/L haemoglobin, 50mg/dL triglyceride;
Blood serum sample 2:50mg/L glycocholic acid (CG), 500mg/L haemoglobin, 10mg/dL triglyceride;
Blood serum sample 3:75mg/L glycocholic acid (CG), 100mg/L haemoglobin, 20mg/dL triglyceride;
Blood serum sample 4:100mg/L glycocholic acid (CG), 150mg/L haemoglobin, 30mg/dL triglyceride;
Blood serum sample 5:150mg/L glycocholic acid (CG), 200mg/L haemoglobin, 40mg/dL triglyceride;
Blank: 50mg/L haemoglobin, 50mg/dL triglyceride;
Glycocholic acid (CG) content data of the detection that sample 1-5 obtains is respectively 24.95mg/L, 50.05mg/L, 74.99mg/L, 99.95mg/L, 150.01mg/L, accuracy affect variation < 10%, do not find when detecting in blank that obvious fluorescence signal changes.
Embodiment 2
The preparation of comparative example test card:
Adopt 25mM glycine buffer pre-service gold mark pad, other reagent and experimental technique are all with embodiment 1.
1) 25mM glycine buffer pre-service gold mark pad is adopted, then with glycocholic acid (CG) the antibody-solutions spraying pretreated gold mark pad of fluorescent microsphere mark, obtained bag is by the gold mark pad of glycocholic acid (CG) antibody, in solution, the mass ratio of fluorescent microsphere and antibody is 5:1, the concentration of solution is 10mg/ml, and quantity for spray is 4ul/cm;
2) on the detection line and nature controlling line of nitrocellulose filter, spray glycocholic acid (CG) antibody-solutions and the sheep anti-mouse antibody solution of 1mg/ml respectively, quantity for spray is 1ul/cm, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter, the adsorptive pads prepared be pasted onto on PVC base plate successively, cutting obtains the Test paper card of wide 3-5mm; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
The sensitivity of glycocholic acid (CG) and detectability contrast experiment:
Judge with fluorescent instrument, analyser be AD value 0-10000 to the sensing range of fluorescence signal, according to the performance of instrument, CUTOFF value is 50, under certain concentration, more than 80% test example AD value >=50, namely think that kit can be used in the detection under this concentration.
Adopt 5%BSA normal saline solution as dummy, dummy should not contain measured object.Glycocholic acid (CG) the known blood sample getting 3 ~ 60pg/mL gradient concentration carries out sensitivity detection, arranges a gradient at interval of 0.2pg/mL, and each gradient arranges 300 samples, record testing result.The lowest detection of the kit of result display embodiment 1 is limited to 3.5pg/mL, and the minimal detectable concentration of the kit of embodiment 2 is higher than 60pg/mL.
In sum, detection kit provided by the present invention has good anti-interference and specificity, and has good sensitivity, and negative background is lower, effectively overcomes various shortcoming of the prior art and tool high industrial utilization.
Above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art scholar all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as have in art usually know the knowledgeable do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.
Claims (10)
1. one kind is quantitatively detected the detection kit of glycocholic acid CG, comprise test card, test card comprises base plate and is positioned at the sample pad be arranged in order from application of sample end of backplate surface, gold mark pad, nitrocellulose filter and adsorptive pads, described gold mark pad comprises CG antibody, described nitrocellulose filter is coated with detection line and nature controlling line, the CG antibody on described gold mark pad adopts fluorescent microsphere mark.
2. the detection kit quantitatively detecting glycocholic acid CG as claimed in claim 1, it is characterized in that, on described nitrocellulose filter, detection line is positioned at side close to application of sample end, and nature controlling line is positioned at side away from application of sample end.
3. the detection kit quantitatively detecting glycocholic acid CG as claimed in claim 1, is characterized in that, described detection line is coated with CG antibody.
4. the detection kit quantitatively detecting glycocholic acid CG as claimed in claim 1, is characterized in that, nature controlling line wraps by sheep anti-mouse antibody.
5. the detection kit quantitatively detecting glycocholic acid CG as claimed in claim 1, it is characterized in that, described sample pad adopts damping fluid process, described damping fluid is selected from one or more the combination in PBS damping fluid, Tris-HCl damping fluid, glycine buffer, borate buffer solution and citrate-phosphate salt buffer, and the concentration of damping fluid is 20-200mM.
6. the detection kit quantitatively detecting glycocholic acid CG as claimed in claim 1, it is characterized in that, described gold mark pad adopts damping fluid process, described damping fluid is selected from one or more combination of glycine buffer, Tris-HCl damping fluid, borate buffer solution, and the concentration of damping fluid is 5-50mM.
7. the detection kit of quantitative detection glycocholic acid CG according to claim 6, it is characterized in that, increased response agent is also comprised in described damping fluid, described increased response agent be selected from PEG4000, PEG6000, PEG8000 and PEG20000 any one, the concentration of described increased response agent is 10 ~ 50g/L.
8. the detection kit quantitatively detecting glycocholic acid CG as claimed in claim 1, it is characterized in that, also comprise and getting stuck, described getting stuck comprises back of the body card and upper cover, the described back of the body is arranged with test card draw-in groove, and described test card is embedded in described test card draw-in groove, is covered with testing window and well on described, matching with the position of described detection line and nature controlling line in the position of described testing window, matches with the position of described sample pad in the position of described well.
9. the detection kit quantitatively detecting fat glycocholic acid CG as claimed in claim 1, is characterized in that, described detection kit is used for the content quantitatively detecting glycocholic acid CG in serum or blood plasma.
10. the preparation method of the detection kit of the quantitative detection fat glycocholic acid CG according to the arbitrary claim of claim 1 ~ 9, specifically comprises the steps:
1) with glycocholic acid (CG) the antibody-solutions spraying pretreated gold mark pad of fluorescent microsphere mark, the obtained gold mark pad comprising glycocholic acid (CG) antibody;
2) on the detection line and nature controlling line of nitrocellulose filter, spray glycocholic acid (CG) antibody and sheep anti-mouse antibody respectively, obtained bag by after nitrocellulose filter;
3) by sample pad, step 1) preparation gold mark pad, step 2) nitrocellulose filter, the adsorptive pads prepared be pasted onto on base plate successively, cutting obtains Test paper card; Finally Test paper is snapped fits into the obtained detection kit that gets stuck.
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| CN201410736270.9A CN104360071B (en) | 2014-12-05 | 2014-12-05 | The kit of content of glycocholic acid in a kind of Fast Measurement serum or blood plasma |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018787A (en) * | 2016-03-31 | 2016-10-12 | 广东工业大学 | Fluorescent enhanced immune analysis method and immune analysis kit for detecting glycocholic acid |
| CN108896756A (en) * | 2018-07-17 | 2018-11-27 | 广东工业大学 | A kind of colloid gold nano-polyaniline-gold nano complex microsphere and its preparation method and application |
| CN110749738A (en) * | 2019-10-30 | 2020-02-04 | 南京佰抗生物科技有限公司 | Kit for detecting gastrin-17 and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050272109A1 (en) * | 2000-02-29 | 2005-12-08 | Jurgen Schaffler | Method for immobilizing conjugates in diagnostic tests |
| CN102879559A (en) * | 2011-07-12 | 2013-01-16 | 上海执诚生物科技股份有限公司 | Real-time quantitative detection reagent and method of time-resolved fluorescence immune chromatography |
| CN102944673A (en) * | 2012-11-30 | 2013-02-27 | 北京华宇亿康生物工程技术有限公司 | Kit (Latex-enhanced immunoturbidimetry) for detecting content of glycocholic acid in blood serum |
| CN103739703A (en) * | 2014-02-11 | 2014-04-23 | 苏州博源医疗科技有限公司 | Glycocholic acid immunogen, anti-glycocholic acid specific antibody and detection reagent |
| CN103760348A (en) * | 2014-02-11 | 2014-04-30 | 苏州博源医疗科技有限公司 | Glycocholic acid immunodetection reagent and preparing method and detecting method thereof |
-
2014
- 2014-12-05 CN CN201410736270.9A patent/CN104360071B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050272109A1 (en) * | 2000-02-29 | 2005-12-08 | Jurgen Schaffler | Method for immobilizing conjugates in diagnostic tests |
| CN102879559A (en) * | 2011-07-12 | 2013-01-16 | 上海执诚生物科技股份有限公司 | Real-time quantitative detection reagent and method of time-resolved fluorescence immune chromatography |
| CN102944673A (en) * | 2012-11-30 | 2013-02-27 | 北京华宇亿康生物工程技术有限公司 | Kit (Latex-enhanced immunoturbidimetry) for detecting content of glycocholic acid in blood serum |
| CN103739703A (en) * | 2014-02-11 | 2014-04-23 | 苏州博源医疗科技有限公司 | Glycocholic acid immunogen, anti-glycocholic acid specific antibody and detection reagent |
| CN103760348A (en) * | 2014-02-11 | 2014-04-30 | 苏州博源医疗科技有限公司 | Glycocholic acid immunodetection reagent and preparing method and detecting method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018787A (en) * | 2016-03-31 | 2016-10-12 | 广东工业大学 | Fluorescent enhanced immune analysis method and immune analysis kit for detecting glycocholic acid |
| CN106018787B (en) * | 2016-03-31 | 2018-07-24 | 广东工业大学 | A kind of the Fluorescence Increasing immunoassay method and immune reagent kit of detection glycocholic acid |
| CN108896756A (en) * | 2018-07-17 | 2018-11-27 | 广东工业大学 | A kind of colloid gold nano-polyaniline-gold nano complex microsphere and its preparation method and application |
| CN108896756B (en) * | 2018-07-17 | 2021-05-11 | 广东工业大学 | Colloidal gold nano-polyaniline-gold nano composite microsphere and preparation method and application thereof |
| CN110749738A (en) * | 2019-10-30 | 2020-02-04 | 南京佰抗生物科技有限公司 | Kit for detecting gastrin-17 and preparation method thereof |
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| Publication number | Publication date |
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| CN104360071B (en) | 2016-03-02 |
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