CN104459008B - A kind of quick detection kit of protein - Google Patents
A kind of quick detection kit of protein Download PDFInfo
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- CN104459008B CN104459008B CN201410742078.0A CN201410742078A CN104459008B CN 104459008 B CN104459008 B CN 104459008B CN 201410742078 A CN201410742078 A CN 201410742078A CN 104459008 B CN104459008 B CN 104459008B
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 107
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 106
- 238000001514 detection method Methods 0.000 title claims abstract description 35
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 87
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000000741 silica gel Substances 0.000 claims abstract description 37
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 37
- 239000002904 solvent Substances 0.000 claims abstract description 31
- 239000000843 powder Substances 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 239000006059 cover glass Substances 0.000 claims abstract description 9
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims abstract description 5
- FSEUPUDHEBLWJY-HWKANZROSA-N diacetylmonoxime Chemical compound CC(=O)C(\C)=N\O FSEUPUDHEBLWJY-HWKANZROSA-N 0.000 claims abstract description 5
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims abstract description 5
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 18
- 229910019142 PO4 Inorganic materials 0.000 claims description 14
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 14
- 239000010452 phosphate Substances 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 claims description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 25
- 238000002331 protein detection Methods 0.000 abstract description 5
- 239000012530 fluid Substances 0.000 abstract description 3
- 235000018102 proteins Nutrition 0.000 description 92
- 239000000376 reactant Substances 0.000 description 20
- 238000013019 agitation Methods 0.000 description 6
- YNQLUTRBYVCPMQ-UHFFFAOYSA-N alpha-methyl toluene Natural products CCC1=CC=CC=C1 YNQLUTRBYVCPMQ-UHFFFAOYSA-N 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 125000000539 amino acid group Chemical group 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 208000002720 Malnutrition Diseases 0.000 description 2
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001071 malnutrition Effects 0.000 description 2
- 235000000824 malnutrition Nutrition 0.000 description 2
- 208000015380 nutritional deficiency disease Diseases 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000009328 Amaranthus caudatus Nutrition 0.000 description 1
- 240000001592 Amaranthus caudatus Species 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 201000002772 Kwashiorkor Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 244000170916 Paeonia officinalis Species 0.000 description 1
- 235000006484 Paeonia officinalis Nutrition 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 235000012735 amaranth Nutrition 0.000 description 1
- 239000004178 amaranth Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
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- 230000007423 decrease Effects 0.000 description 1
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- 238000004090 dissolution Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 208000037921 secondary disease Diseases 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a kind of quick detection kit and using method thereof of protein, be applicable to the fast detecting of protein in protein powder. The quick detection kit of protein comprises following composition: solvent, filter paper, buffer, developer, solvent, standard protein methanol solution, quantitatively 1g protein powder spoon, capillary, 25x15 silica gel plate and have cover glass bottle. Whether the present invention is by containing protein in the reaction filtered fluid of biacetyl monoxime/nickel chloride and the simple and quick detection sample of the chromogenic reaction of protein, the quick detection kit of protein provided by the invention, simple to operation, speed is fast, detect successful, recall rate high, for protein detection provides new standard.
Description
Technical field
The invention belongs to fast detecting field, be specifically related to a kind of quick detection kit and using method thereof of protein.
Background technology
Protein is the material base of life, is organic macromolecule, is the basic organic matter that forms cell, is the main undertaker of vital movement. There is no life without protein. Amino acid is the basic composition unit of protein. It is the material together with being closely connected with life and with various forms of vital movements. Each cell in body and all important component parts have protein to participate in. Protein accounts for 16% ~ 20% of human body weight, in its body of adult that 60kg is heavy, approximately has protein 9.6 ~ 12kg. The kind of human body internal protein is a lot, character, Various Functions, but all combined by different proportion by 20 several amino acids (Aminoacid), and constantly carry out metabolism and renewal in vivo. Protein is a kind of organic compound of complexity, is once called as protein. Amino acid is the base unit of constitutive protein matter, and amino acid is linked to be peptide chain by dehydrating condensation. The large biological molecule that protein is made up of one or more polypeptide chain, each polypeptide chain does not have 20 to hundreds of amino acid residues (R) not etc.; Various amino acid residues are arranged in sequence. The amino acid sequence of protein is by corresponding coded by said gene. Except 20 kinds of coded primary amino acids of genetic code, in protein, some amino acid residue can also be translated rear modification and the variation of chemical constitution occurs, thereby protein is activated or regulated and controled. Multiple protein can be together, and often by being combined together to form stable protein complex, folding or spiral forms certain space structure, thereby brings into play a certain specific function. The organelle of synthetic polypeptide is the ribosomes in exasperate type endoplasmic reticulum in cytoplasm. The difference of protein is its amino acid whose kind, number, put in order and the difference of peptide chain space structure. Potein deficiency has generation in adult and children, but children in growth phase are more responsive. The shortage common sympton of protein is that metabolic rate declines, disease resistance is gone down, easily ill, long-term effect is the infringement of organ, commonly children's growth retardation, malnutrition, weight decline, indifferent, easily enrage, anaemia and dry-sickness or oedema, and because easy infection and secondary disease. The shortage of protein, is often protein-heat energy malnutrition with common existence of the shortage of energy again, is divided into two kinds, a kind ofly refers to that heat energy is taken in basic meeting and the nutritive disease of protein wretched insufficiency claims kwashiorkor. Another kind is " becoming thin ", and finger protein matter and heat energy are taken in the nutritive disease of equal wretched insufficiency. Protein is the indispensable material of human body, deceives people consumer and has obtained juice but many illegal retailers in this year manufacture false albumen.
At present, it is good several that traditional protein detection method mainly contains Kjeldahl's method, coomassie brilliant blue (Bradford method), Folin-phenol reagent process, biuret method etc., but known protein detection method respectively has again its defect. Kjeldahl's method is time-consuming oversize for the Accurate Determining of standard protein content; Biuret method is used for Fast Measurement but is not too sensitive; Different proteins colour developing is similar; Folin-phenol reagent process length that expends time in, strict timing is wanted in operation, and shade changes with different proteins, and calibration curve is not strict form of straight lines, and selectivity is poor; Coomassie brilliant blue mixed protein is difficulty quantitatively. Therefore, find a kind of protein detection reagents that can be simple and economical and practical fast very urgent.
Summary of the invention
The object of the invention is to provide in order to overcome above the deficiencies in the prior art a kind of economical and practical, detection effect is measured quick detection kit and the using method thereof of protein accurately.
Technical scheme: the invention discloses a kind of quick detection kit of protein, comprise following composition: solvent, filter paper, buffer, developer, solvent, standard protein methanol solution, quantitatively 1g protein powder spoon, capillary, 25x15 silica gel plate and have cover glass bottle.
The quick detection kit of above-described protein, solvent is ethanol.
The quick detection kit of above-described protein, the phosphate solution that buffer is 50 ~ 200mmol/L.
The quick detection kit of above-described protein, the reaction filtered fluid that developer is biacetyl monoxime/nickel chloride.
The quick detection kit of above-described protein, developer is that 1.0g biacetyl monoxime is dissolved in DEG C water of 30mL50 ~ 80, adds 0.8g nickel chloride, adds 2mL concentrated ammonia liquor after cooling, stir the hydroxylamine hydrochloride solution that adds 200mL0.5g/mL after 5 minutes, stir the filtrate of filtering for 12 ~ 24 hours.
The quick detection kit of above-described protein, solvent is that volume ratio is the mixed liquor of methyl alcohol, ethyl acetate and the benzene of 1:10:50.
The quick detection kit of above-described protein, standard protein concentration of methanol solution is 0.2 ~ 1.5mol/L.
The using method of the quick detection kit of above-described protein, comprises the steps:
Step 1, gets 1g testing protein powder sample, adds 5-10mL dissolution with solvents, passes through Filter paper filtering after adding thermal agitation 2-3 minute;
Step 2, adds 1-2 to drip to drip 3-5 after buffer to the filtrate described in step 1 and drips developer, stirs and detects by TLC after 3-5 minute;
Step 3, TLC described in step 3 is to get the reactant liquor described in step 2 with capillary, point sample is on silica gel plate, the methanol solution contrast point sample of standard protein, vertically put into the vial that solvent is housed, treat that solvent extends to from prolonging 3 centimeters on silica gel plate and take out silica gel plates and dry up, dry observe phenomena with fire;
Step 4, contains a large amount of protein in sample if the observe phenomena described in step 3 is the point of reactant liquor on silica gel plate and the aobvious bronzing of point of the point of contrast sample in equal height and reactant liquor; Do not contain on the contrary protein or a small amount of protein.
Beneficial effect: whether the present invention is by containing protein in the reaction filtered fluid of biacetyl monoxime/nickel chloride and the simple and quick detection sample of the chromogenic reaction of protein, the quick detection kit of protein provided by the invention, simple to operation, speed is fast, detect successful, recall rate high, for protein detection provides new standard.
Detailed description of the invention
Embodiment 1
A quick detection kit for protein, comprises following composition: the phosphate solution of ethanol, filter paper, 50mmol/L, developer, solvent (mixed liquor of methyl alcohol, ethyl acetate and benzene that volume ratio is 1:10:50), quantitatively protein methanol solution, capillary, the 25x15 of 1g protein powder spoon, 1.5mol/L silica gel plate and have cover glass bottle.
The quick detection kit of above-described protein and the using method of using method thereof, comprise the steps:
Step 1, gets 1g testing protein powder sample, adds 5mL ethanol to dissolve, and adds thermal agitation and passes through Filter paper filtering after 3 minutes;
Step 2 drips 5 developers after the filtrate described in step 1 adds 2 phosphate solutions, stirs and detects by TLC after 3 minutes;
Step 3, TLC described in step 3 is to get the reactant liquor described in step 2 with capillary, point sample is on silica gel plate, the methanol solution contrast point sample of standard protein, vertically put into the vial that solvent is housed, treat that solvent extends to from prolonging 3 centimeters on silica gel plate and take out silica gel plates and dry up, dry observe phenomena with fire;
Step 4, the phenomenon described in step 3 is that the point of reactant liquor on silica gel plate contains a large amount of protein in sample with the point of contrast sample in the some displaing amaranth of equal height and reactant liquor.
Embodiment 2
A kind of quick detection kit of protein and, comprise following composition: the phosphate solution of ethanol, filter paper, 200mmol/L, developer, solvent (mixed liquor of methyl alcohol, ethyl acetate and benzene that volume ratio is 1:10:50), quantitatively protein methanol solution, capillary, the 25x15 of 1g protein powder spoon, 0.2mol/L silica gel plate and have cover glass bottle.
The quick detection kit of above-described protein and the using method of using method thereof, comprise the steps:
Step 1, gets 1g testing protein powder sample, adds 10mL ethanol to dissolve, and adds thermal agitation and passes through Filter paper filtering after 3 minutes;
Step 2 drips 5 developers after the filtrate described in step 1 adds 1 phosphate solution, stirs and detects by TLC after 5 minutes;
Step 3, TLC described in step 3 is to get the reactant liquor described in step 2 with capillary, point sample is on silica gel plate, the methanol solution contrast point sample of standard protein, vertically put into the vial that solvent is housed, treat that solvent extends to from prolonging 3 centimeters on silica gel plate and take out silica gel plates and dry up, dry observe phenomena with fire;
Step 4, the phenomenon described in step 3 is that the point of reactant liquor on silica gel plate does not contain or contain a small amount of protein in equal height with the point of contrast sample.
Embodiment 3
A quick detection kit for protein, comprises following composition: the phosphate solution of methyl alcohol, filter paper, 150mmol/L, developer, solvent (mixed liquor of methyl alcohol, ethyl acetate and benzene that volume ratio is 1:10:50), quantitatively protein methanol solution, capillary, the 25x15 of 1g protein powder spoon, 1.0mol/L silica gel plate and have cover glass bottle.
The quick detection kit of above-described protein and the using method of using method thereof, comprise the steps:
Step 1, gets 1g testing protein powder sample, adds 8mL ethanol to dissolve, and adds thermal agitation and passes through Filter paper filtering after 3 minutes;
Step 2 drips 4 developers after the filtrate described in step 1 adds 2 phosphate solutions, stirs and detects by TLC after 4 minutes;
Step 3, TLC described in step 3 is to get the reactant liquor described in step 2 with capillary, point sample is on silica gel plate, the methanol solution contrast point sample of standard protein, vertically put into the vial that solvent is housed, treat that solvent extends to from prolonging 3 centimeters on silica gel plate and take out silica gel plates and dry up, dry observe phenomena with fire;
Step 4, the phenomenon described in step 3 is that the point of reactant liquor on silica gel plate and the point aobvious pale red of the point of contrast sample in equal height and reactant liquor contain a small amount of protein in sample.
Embodiment 4
A quick detection kit for protein, comprises following composition: the phosphate solution of methyl alcohol, filter paper, 160mmol/L, developer, solvent (mixed liquor of methyl alcohol, ethyl acetate and benzene that volume ratio is 1:10:50), quantitatively protein methanol solution, capillary, the 25x15 of 1g protein powder spoon, 0.6mol/L silica gel plate and have cover glass bottle.
The quick detection kit of above-described protein and the using method of using method thereof, comprise the steps:
Step 1, gets 1g testing protein powder sample, adds 9mL ethanol to dissolve, and heating is stirred after 3 minutes and passed through Filter paper filtering;
Step 2 drips 5 developers after the filtrate described in step 1 adds 1 phosphate solution, stirs and detects by TLC after 5 minutes;
Step 3, TLC described in step 3 is to get the reactant liquor described in step 2 with capillary, point sample is on silica gel plate, the methanol solution contrast point sample of standard protein, vertically put into the vial that solvent is housed, treat that solvent extends to from prolonging 3 centimeters on silica gel plate and take out silica gel plates and dry up, dry observe phenomena with fire;
Step 4, the phenomenon described in step 3 is that the point of reactant liquor on silica gel plate and the point aobvious peony of the point of contrast sample in equal height and reactant liquor contain a large amount of protein in sample.
Embodiment 5
A quick detection kit for protein, comprises following composition: the phosphate solution of methyl alcohol, filter paper, 200mmol/L, developer, solvent (mixed liquor of methyl alcohol, ethyl acetate and benzene that volume ratio is 1:10:50), quantitatively protein methanol solution, capillary, the 25x15 of 1g protein powder spoon, 1.3mol/L silica gel plate and have cover glass bottle.
The quick detection kit of above-described protein and the using method of using method thereof, comprise the steps:
Step 1, gets 1g testing protein powder sample, adds 6mL ethanol to dissolve, and adds thermal agitation and passes through Filter paper filtering after 2 minutes;
Step 2 drips 4 developers after the filtrate described in step 1 adds 1 phosphate solution, stirs and detects by TLC after 3 minutes;
Step 3, TLC described in step 3 is to get the reactant liquor described in step 2 with capillary, point sample is on silica gel plate, the methanol solution contrast point sample of standard protein, vertically put into the vial that solvent is housed, treat that solvent extends to from prolonging 3 centimeters on silica gel plate and take out silica gel plates and dry up, dry observe phenomena with fire;
Step 4, the phenomenon described in step 3 is that the point of reactant liquor on silica gel plate and the point aobvious reddish black of the point of contrast sample in equal height and reactant liquor contain a large amount of protein in sample.
Embodiment 6
A quick detection kit for protein, comprises following composition: the phosphate solution of ethanol, filter paper, 75mmol/L, developer, solvent (mixed liquor of methyl alcohol, ethyl acetate and benzene that volume ratio is 1:10:50), quantitatively protein methanol solution, capillary, the 25x15 of 1g protein powder spoon, 0.3mol/L silica gel plate and have cover glass bottle.
The quick detection kit of above-described protein and the using method of using method thereof, comprise the steps:
Step 1, gets 1g testing protein powder sample, adds 5mL ethanol to dissolve, and adds thermal agitation and passes through Filter paper filtering after 3 minutes;
Step 2 drips 3 developers after the filtrate described in step 1 adds 2 phosphate solutions, stirs and detects by TLC after 5 minutes;
Step 3, TLC described in step 3 is to get the reactant liquor described in step 2 with capillary, point sample is on silica gel plate, the methanol solution contrast point sample of standard protein, vertically put into the vial that solvent is housed, treat that solvent extends to from prolonging 3 centimeters on silica gel plate and take out silica gel plates and dry up, dry observe phenomena with fire;
Step 4, the phenomenon described in step 3 be the point of reactant liquor on silica gel plate and the point of contrast sample not the aobvious pale red of the point in equal height and reactant liquor in sample, do not contain or a small amount of protein.
The protein powder for the random different brands that each large, medium and small supermarket, shop are bought in Suzhou that above embodiment 1-6 detects, delivers the protein powder of detection to regular testing agency to detect, and comparing result is in table 1:
The testing result of table 1 embodiment 1-6 and the testing result comparison of regular testing agency
As known from Table 1, the quick detection kit of protein used in the present invention and using method thereof detect effect and authorize testing agency's testing result identical with country, have cost low simultaneously, make simply, and detection sensitivity is high, simple and quick advantage.
Claims (1)
1. the quick detection kit of a protein, it is characterized in that, comprise following composition: solvent, filter paper, buffer, developer, solvent, standard protein methanol solution, quantitatively 1g protein powder spoon, capillary, 25 × 15 silica gel plate and have cover glass bottle;
Solvent is ethanol;
Buffer is the phosphate solution of 50 ~ 200mmol/L;
Developer is that 1.0g biacetyl monoxime is dissolved in DEG C water of 30mL50 ~ 80, adds 0.8g nickel chloride, adds 2mL concentrated ammonia liquor after cooling, stirs the hydroxylamine hydrochloride solution that adds 200mL0.5g/mL after 5 minutes, stirs the filtrate of filtering for 12 ~ 24 hours;
Solvent is that volume ratio is the mixed liquor of methyl alcohol, ethyl acetate and the benzene of 1:10:50;
Standard protein concentration of methanol solution is 0.2 ~ 1.5mol/L.
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| CN105651765A (en) * | 2015-12-28 | 2016-06-08 | 东北农业大学 | Rapid protein test paper, and production method and detection method thereof |
| CN106525829A (en) * | 2016-10-31 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Test paper for protein rapid detection and preparation method thereof |
| CN113607719B (en) * | 2016-11-29 | 2024-10-22 | 普诺森公司 | Method and apparatus for simultaneous detection of a wide range of protein concentrations |
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