CN102183614A - Method for detecting glycosylated hemoglobin by using thin layer chromatography or paper chromatography - Google Patents
Method for detecting glycosylated hemoglobin by using thin layer chromatography or paper chromatography Download PDFInfo
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- CN102183614A CN102183614A CN2011100329123A CN201110032912A CN102183614A CN 102183614 A CN102183614 A CN 102183614A CN 2011100329123 A CN2011100329123 A CN 2011100329123A CN 201110032912 A CN201110032912 A CN 201110032912A CN 102183614 A CN102183614 A CN 102183614A
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Abstract
The invention discloses a method for detecting glycosylated hemoglobin by using thin layer chromatography or paper chromatography. Mainly instruments are used for detection in the conventional methods for detecting the glycosylated hemoglobin; an electrophoresis method, a micro column chromatography method and an immune turbidimetry method are widely applied in early detection; and at present, a high performance liquid chromatography method, an enzyme method and a chemiluminescence method (IVIA method) for detecting are applied in clinic. The method is a new method for detecting the glycosylated hemoglobin, namely the glycosylated hemoglobin and non-glycosylated hemoglobin are separated according to different charges carried by the glycosylated hemoglobin and the non-glycosylated hemoglobin by using the thin layer chromatography or the paper chromatography, and meanwhile, the glycosylated hemoglobin is detected by using visual color comparison and manual measurement methods according to the principle that the color (or graph or color spot, the same below) depth and area of the glycosylated hemoglobin on the surface of a thin layer chromatography or paper chromatography medium are in proportion to the content of the glycosylated hemoglobin. The method is cheap and convenient for popularization. The method is simple, and does not need to separate hemoglobin because whole blood is used. Instruments and power supplies are not used in the method, and the detection performance of the method is equivalent to that of other methods.
Description
Technical field
The present invention is a kind of method of new detection glycosylated hemoglobin, uses thin-layer chromatography or ply of paper to analyse under the situation that does not need power supply and separates saccharification and non-glycosylated hemoglobin, uses the method for visual colorimetry and hand dipping to detect glycosylated hemoglobin then.
Background technology
The method that detects glycosylated hemoglobin at present mainly is to use instrument detecting, early detection is used more extensive with electrophoresis, microtrabeculae chromatography, immunoturbidimetry, high performance liquid chromatography, enzyme process and chemoluminescence method (IVIA method) detect and begin in clinical practice at present.
Ultimate principle of the present invention and method are to use thin-layer chromatography or ply of paper to analyse and estimate detection, and the advantage one of the method is cheap, is convenient to popularize.The 2nd, simple, use whole blood, need not separate haemoglobin.The 3rd, without instrument and power supply, it is suitable with other method that it detects performance.
Summary of the invention
The present invention is charged different with non-glycosylated hemoglobin institute according to glycosylated hemoglobin, the method of utilizing thin-layer chromatography or ply of paper to analyse is separated it, simultaneously according to its thin-layer chromatography or ply of paper analyse that dielectric surface forms color (or collection of illustrative plates or color spot, the down with) depth and the principle that is directly proportional with its content of area size it is carried out detection by quantitative.The method that detects has two kinds: the one, and colourimetry is carried out color and the glycosylated hemoglobin after the chromatography that forms in chromatographic medium surface before the detected whole blood sample chromatography visual colorimetry and is calculated the ratio of glycosylated hemoglobin in total hemoglobin that detect in the color of chromatographic medium surface formation and the Standard Colors of standard haemoglobin.The 2nd, saccharification by measuring calculation sample and non-glycosylated hemoglobin are analysed the area that dielectric surface forms at thin-layer chromatography or ply of paper and are detected the ratio of glycosylated hemoglobin in total hemoglobin.
Embodiment
1. at first the standard blood of known different hemoglobin concentration is got equal volume and is added the surface of chromatography media, treat its immerse fully the back accurately its formation of survey record the blood cake color and with it as Standard Colors.
2. the color spot color that sample to be detected is formed on the surface of chromatography media before chromatography not and the total hemoglobin concentration of Standard Colors colorimetric detection sample.
3. add moving phase afterwards and carry out chromatography, contain hemolytic agent in the moving phase, blood with haemolysis promptly to take place after moving phase contacts discharge haemoglobin, glycosylated hemoglobin moves forward with moving phase.
4. chromatography end back promptly is a glycosylated hemoglobin at the top color spot of chromatography collection of illustrative plates, with its color and Standard Colors colorimetric detection glycosylated hemoglobin concentration, the concentration of glycosylated hemoglobin promptly is the ratio of glycosylated hemoglobin in total hemoglobin divided by the concentration of total hemoglobin.
5. chromatography end back promptly is a glycosylated hemoglobin at the top color spot of chromatography collection of illustrative plates, the back is non-glycosylated hemoglobin, calculate the color spot area that glycosylated hemoglobin and non-glycosylated hemoglobin form respectively, the glycosylated hemoglobin area promptly is the ratio of glycosylated hemoglobin in total hemoglobin divided by non-glycosylated hemoglobin area and glycosylated hemoglobin area sum.
Claims (5)
1. method of Shi Yonging or principle are that thin-layer chromatography or ply of paper are analysed.
2. method or the principle of using according to claim 1. is visual colorimetry, and color and the glycosylated hemoglobin after the chromatography that forms in chromatographic medium surface before the detected whole blood sample chromatography carried out visual colorimetry and calculate the ratio of glycosylated hemoglobin in total hemoglobin that detect with the color of standard haemoglobin respectively in the color that chromatographic medium surface forms.
3. method of using according to claim 1. or principle are that chromatography finishes saccharification and the non-glycosylated hemoglobin of back by measuring calculation sample and analyses the color spot area that dielectric surface forms at thin-layer chromatography or ply of paper and detect the ratio of glycosylated hemoglobin in total hemoglobin.
4. the moving phase of using according to claim 1. chromatography contains hemolytic agent, and moving phase has 2 kinds of effects of haemolysis and chromatography.
5. the sample of Jian Ceing is all haemoglobins after whole blood or the separation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011100329123A CN102183614A (en) | 2011-01-31 | 2011-01-31 | Method for detecting glycosylated hemoglobin by using thin layer chromatography or paper chromatography |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100329123A CN102183614A (en) | 2011-01-31 | 2011-01-31 | Method for detecting glycosylated hemoglobin by using thin layer chromatography or paper chromatography |
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| CN102183614A true CN102183614A (en) | 2011-09-14 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104459008A (en) * | 2014-12-09 | 2015-03-25 | 苏州东辰林达检测技术有限公司 | Rapid detection kit for protein and using method of kit |
| CN105203528A (en) * | 2015-09-22 | 2015-12-30 | 深圳市希莱恒医用电子有限公司 | Glycosylated hemoglobin detecting device |
| CN107202897A (en) * | 2017-05-22 | 2017-09-26 | 海格德生物科技（深圳）有限公司 | Detection method, chromatograph test strip and its assemble method of glycosylated hemoglobin |
| CN108287210A (en) * | 2018-01-13 | 2018-07-17 | 常州大学 | Mast cell chymotrypsin micro rapid detection method in vitro |
| CN113295830A (en) * | 2021-05-31 | 2021-08-24 | 黄埔海关技术中心 | Simple wine caramel pigment detection robot and detection method |
-
2011
- 2011-01-31 CN CN2011100329123A patent/CN102183614A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104459008A (en) * | 2014-12-09 | 2015-03-25 | 苏州东辰林达检测技术有限公司 | Rapid detection kit for protein and using method of kit |
| CN104459008B (en) * | 2014-12-09 | 2016-05-11 | 山东拜尔检测有限公司 | A kind of quick detection kit of protein |
| CN105203528A (en) * | 2015-09-22 | 2015-12-30 | 深圳市希莱恒医用电子有限公司 | Glycosylated hemoglobin detecting device |
| CN107202897A (en) * | 2017-05-22 | 2017-09-26 | 海格德生物科技（深圳）有限公司 | Detection method, chromatograph test strip and its assemble method of glycosylated hemoglobin |
| CN107202897B (en) * | 2017-05-22 | 2021-01-19 | 海格德生物科技（深圳）有限公司 | Detection method of glycosylated hemoglobin, chromatographic test strip and assembly method thereof |
| CN108287210A (en) * | 2018-01-13 | 2018-07-17 | 常州大学 | Mast cell chymotrypsin micro rapid detection method in vitro |
| CN113295830A (en) * | 2021-05-31 | 2021-08-24 | 黄埔海关技术中心 | Simple wine caramel pigment detection robot and detection method |
| CN113295830B (en) * | 2021-05-31 | 2021-12-07 | 黄埔海关技术中心 | Simple wine caramel pigment detection robot and detection method |
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Application publication date: 20110914 |