CN104435142A - Extraction method of buckwheat flavonoid - Google Patents
Extraction method of buckwheat flavonoid Download PDFInfo
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- CN104435142A CN104435142A CN201410811107.4A CN201410811107A CN104435142A CN 104435142 A CN104435142 A CN 104435142A CN 201410811107 A CN201410811107 A CN 201410811107A CN 104435142 A CN104435142 A CN 104435142A
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- Prior art keywords
- radix
- rhizoma fagopyri
- fagopyri tatarici
- buckwheat
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The invention relates to an extraction method of buckwheat flavonoid. The extracted buckwheat flavonoid is high in purity and high in extraction efficiency. The method comprises the following steps: (1) adding one or more of buckwheat peel, buckwheat stems or buckwheat seeds to an extractor, extracting with a propyl alcohol solution at 0.3-0.4MPa and 50-70 DEG C, and filtering extract liquid; (2) evaporating and concentrating the filtrate at 0.2-0.3MPa and 60-70 DEG C; and (3) mixing the concentrated liquid with water at the weight ratio of 1 to (3-5), stirring, adding an organic solvent 2-methyl-butane, extracting, taking lower suspension, centrifugally separating, and drying, so as to obtain the purified buckwheat flavonoid. The extraction method is high in yield; and the product is high in purity.
Description
Technical field
The present invention relates to a kind of extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone, its Radix Et Rhizoma Fagopyri Tatarici flavone purity extracted is high, and extraction efficiency is high.
Background technology
Radix Et Rhizoma Fagopyri Tatarici is the medicine-food two-purpose crop that occurring in nature is very few, records according to Compendium of Material Medica: Radix Et Rhizoma Fagopyri Tatarici bitter in the mouth, and property is flat cold, the real the intestines and stomach of energy, physical strength profiting, continuous spirit, sharp knowledge, and refining the five internal organs slag is dirty; All on the books to Radix Et Rhizoma Fagopyri Tatarici in Thousand Golden Prescriptions, " Chinese medicine voluminous dictionary " and pertinent literature: can calm the nerves, wind pain is swollen in the wide intestinal of lively atmosphere blood, sending down the abnormal ascending QI, heat clearing away, long-pendingization of dispelling is stagnant, bowel relieving, intestine moistening, relieving constipation, cough-relieving, relieving asthma, antiinflammatory, antiallergic, heart tonifying, fat-reducing, the effect such as beauty treatment
Radix Et Rhizoma Fagopyri Tatarici and Radix Et Rhizoma Fagopyri Tatarici, formal name used at school hull buckwheat (Tartar, pronunciation d á d á is the general designation to Chinese ancient northern minority name race), another name buckwheat leaf seven, Radix Rhapontici buckwheat, Wan Nianqiao, spinach wheat, Wu Mai, Hua Qiao.Exceed much than the nutritive value of sweet buckwheat and Semen Fagopyri Esculenti.Particularly the content of bioflavonoids is 13.5 times of Semen Fagopyri Esculenti.
The unique activity composition of Radix Et Rhizoma Fagopyri Tatarici---Radix Et Rhizoma Fagopyri Tatarici flavone a kind ofly has the more complicated plyability material of diverse biological activities, Main Ingredients and Appearance has Quercetin, rutin, bank luxuriant and rich with fragrance alcohol, morin, wherein rutin accounts for 80%, and the ratio of this natural composition more has cooperative effect, is more easily absorbed by the body.It has heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, embolism resistance, improves microcirculation, can effectively reduce blood capillary fragility and permeability, has prevention cerebral microvascular hemorrhage and safeguard the effect that eye circulates, four large physiologically actives:
1. blood circulation promoting and blood stasis dispelling, reduction cholesterol, Radix Et Rhizoma Fagopyri Tatarici flavone, to coronary heart disease, cardiovascular and cerebrovascular vessel and peripheral angiopathy, all has good therapeutical effect.Its blood circulation promoting and blood stasis dispelling, improve microcirculatory effective percentage and reach 88%.
2. sterilize, antibacterial, the Radix Et Rhizoma Fagopyri Tatarici solution of 0.08% can kill escherichia coli, bacillus subtilis, staphylococcus aureus in 8 hours, and killing rate reaches 85%, can kill noxious bacteria in body, can prevent to regulate the intestines and stomach inflammation.
3. antioxidation, anti-cancer, Radix Et Rhizoma Fagopyri Tatarici flavone can prevent oils and fats autoxidation.The activation of cell for cancer can be pressed down in addition, prevent the hypertrophy of tumor, reach the effect of anti-cancer.
4. glucagon resistance factor, Radix Et Rhizoma Fagopyri Tatarici flavone can effectively resist " resistin ", improve cell, organize sensitivity to insulin, strengthen the self-repairing capability of cell tissue, thus the dual regulation reached diabetes, high blood glucose can be made to reduce, and low blood glucose raises, and normal blood glucose is constant.Really reaching green, safely prevention and corntrol diabetes and complication thereof, is natural glucokinin.
Existing Radix Et Rhizoma Fagopyri Tatarici flavone extracting method mostly divide three times, extract all at 4 hours at every turn, extracted rear merge extractive liquid, filter etc., exist on extraction time, efficiency is low, and the purity of the Radix Et Rhizoma Fagopyri Tatarici flavone of extraction is low, the problem that production cost is large.
Summary of the invention
It is high that the technical problem to be solved in the present invention is to provide a kind of yield, the extracting method of the Radix Et Rhizoma Fagopyri Tatarici flavone that product purity is large.
Content of the present invention comprises the steps:
(1) add in extractor by one or more in KUQIAOPI, Radix Et Rhizoma Fagopyri Tatarici bar or Radix Et Rhizoma Fagopyri Tatarici seed, be 0.3-0.4MPa by propanol solution at pressure, temperature is extract under 50-70 DEG C of condition, filters extracting solution;
(2) by filtrate at 0.2-0.3MPa, temperature is evaporation and concentration under 60-70 DEG C of condition;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1: 3-5, stir, add organic solvent 2-methyl-butan and extract, take off a layer suspension, centrifugalize, dry, obtain the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.
Extraction time in described step (1) is 2-3 hour.
The evaporation and concentration time of described step (2) is 2-3 hour.
The number of times that described step (3) carries out extracting is 3-5 time.
The addition of the middle organic solvent of described step (3) is 1-3 times of concentrated solution weight.
The invention has the beneficial effects as follows, raw material of the present invention to carry out source range wide, these are rich in the raw material of Radix Et Rhizoma Fagopyri Tatarici flavone can to utilize the skin of Radix Et Rhizoma Fagopyri Tatarici, bar and seed, by utilizing organic solvent propanol to extract, filter, evaporation and concentration, finally extract, be separated, drying obtains Radix Et Rhizoma Fagopyri Tatarici flavone.The response time of whole process is short, the shortlyest can be 5 hours, and extraction efficiency is high, and the purity of the product of extraction is high, and purity can reach 95%, and the production cost of whole product is reduced greatly.
Detailed description of the invention
Embodiment 1
The present invention includes following steps:
(1) add in extractor by KUQIAOPI, Radix Et Rhizoma Fagopyri Tatarici bar and Radix Et Rhizoma Fagopyri Tatarici seed, be 0.3MPa by propanol solution at pressure, temperature is extract under 65 DEG C of conditions, and extraction time is 2 hours, filters extracting solution;
(2) by filtrate at 0.3MPa, temperature is evaporation and concentration under 70 DEG C of conditions, and concentration time is 3 hours;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1: 4, stir, the organic solvent 2-methyl-butan adding 2 times of concentrated solution weight extracts, take off a layer suspension, again add organic solvent 2-methyl-butan and extract, centrifugalize, drying, obtains the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.Efficient liquid phase chromatographic analysis obtains, and the purity of Radix Et Rhizoma Fagopyri Tatarici flavone is 98.2%.
Embodiment 2
The present invention includes following steps:
(1) add in extractor by KUQIAOPI and Radix Et Rhizoma Fagopyri Tatarici bar, be 0.4MPa by propanol solution at pressure, temperature is extract under 65 DEG C of conditions, and extraction time is 3 hours, filters extracting solution;
(2) by filtrate at 0.2MPa, temperature is evaporation and concentration under 60 DEG C of conditions, and concentration time is 3 hours;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1: 3, stir, the organic solvent 2-methyl-butan adding 1 times of concentrated solution weight extracts, take off a layer suspension, again add organic solvent 2-methyl-butan and carry out extraction 4 times, centrifugalize, dry, obtain the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.Efficient liquid phase chromatographic analysis obtains, and the purity of Radix Et Rhizoma Fagopyri Tatarici flavone is 97.1%.
Embodiment 3
The present invention includes following steps:
(1) add in extractor by Radix Et Rhizoma Fagopyri Tatarici seed, be 0.35MPa by propanol solution at pressure, temperature is extract under 70 DEG C of conditions, and extraction time is 2.5 hours, filters extracting solution;
(2) by filtrate at 0.3MPa, temperature is evaporation and concentration under 65 DEG C of conditions, and concentration time is 2 hours;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1: 5, stir, the organic solvent 2-methyl-butan adding 3 times of concentrated solution weight extracts, take off a layer suspension, again add organic solvent 2-methyl-butan and carry out extraction 2 times, centrifugalize, dry, obtain the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.Efficient liquid phase chromatographic analysis obtains, and the purity of Radix Et Rhizoma Fagopyri Tatarici flavone is 96.5%.
Claims (5)
1. an extracting method for Radix Et Rhizoma Fagopyri Tatarici flavone, is characterized in that, comprises the steps:
(1) add in extractor by one or more in KUQIAOPI, Radix Et Rhizoma Fagopyri Tatarici bar or Radix Et Rhizoma Fagopyri Tatarici seed, be 0.3-0.4MPa by propanol solution at pressure, temperature is extract under 50-70 DEG C of condition, filters extracting solution;
(2) by filtrate at 0.2-0.3MPa, temperature is evaporation and concentration under 60-70 DEG C of condition;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1: 3-5, stir, add organic solvent 2-methyl-butan and extract, take off a layer suspension, centrifugalize, dry, obtain the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.
2. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1, is characterized in that, the extraction time in described step (1) is 2-3 hour.
3. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1, is characterized in that, the evaporation and concentration time of described step (2) is 2-3 hour.
4. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1, is characterized in that, the number of times that described step (3) carries out extracting is 3-5 time.
5. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1, is characterized in that, the addition of the middle organic solvent of described step (3) is 1-3 times of concentrated solution weight.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410811107.4A CN104435142A (en) | 2014-12-13 | 2014-12-13 | Extraction method of buckwheat flavonoid |
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| CN201410811107.4A CN104435142A (en) | 2014-12-13 | 2014-12-13 | Extraction method of buckwheat flavonoid |
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| CN104435142A true CN104435142A (en) | 2015-03-25 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107227251A (en) * | 2017-07-25 | 2017-10-03 | 山西省农业科学院高粱研究所 | A kind of mature vinegar for being enriched with polyphenol compound and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040202733A1 (en) * | 2001-07-12 | 2004-10-14 | Yatcilla Michael T. | Food products and dietary supplements containing phenolated proteins and process for preparing the same |
| JP2008092922A (en) * | 2006-10-06 | 2008-04-24 | Tokyo Univ Of Agriculture | Method for producing flavone |
| CN103040951A (en) * | 2012-09-07 | 2013-04-17 | 赵立地 | Method for extracting buckwheat flavonoids |
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2014
- 2014-12-13 CN CN201410811107.4A patent/CN104435142A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040202733A1 (en) * | 2001-07-12 | 2004-10-14 | Yatcilla Michael T. | Food products and dietary supplements containing phenolated proteins and process for preparing the same |
| JP2008092922A (en) * | 2006-10-06 | 2008-04-24 | Tokyo Univ Of Agriculture | Method for producing flavone |
| CN103040951A (en) * | 2012-09-07 | 2013-04-17 | 赵立地 | Method for extracting buckwheat flavonoids |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107227251A (en) * | 2017-07-25 | 2017-10-03 | 山西省农业科学院高粱研究所 | A kind of mature vinegar for being enriched with polyphenol compound and preparation method thereof |
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