CN104434622A - Novel function and application of seville orange flower extract - Google Patents
Novel function and application of seville orange flower extract Download PDFInfo
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- CN104434622A CN104434622A CN201410613299.8A CN201410613299A CN104434622A CN 104434622 A CN104434622 A CN 104434622A CN 201410613299 A CN201410613299 A CN 201410613299A CN 104434622 A CN104434622 A CN 104434622A
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Abstract
The invention relates to a novel function and application of seville orange flower extract. Particularly, hesperidin, neohesperidin and other flavonoid substances in the seville orange flower extract are capable of promoting cell proliferation and expression of mRNA of fibrocyte IV-type collagen, inhibiting activities of elastase and matrix metalloproteinase, reducing damage of ultraviolet ray to cells and reducing the level of reactive oxygen species and secretion of inflammatory factors, so that the seville orange flower extract has a function of resisting radiation and aging for skins. Therefore, the invention provides a new direction of seville orange flower extract application, namely application of the seville orange flower extract in skin protecting products.
Description
Technical field
The present invention relates to a kind of New function of CitrusaurantiumL.Var.amara Engl. extract, specifically the radioprotective of CitrusaurantiumL.Var.amara Engl. extract, antidotal New function and application thereof.
Background technology
Belong to the mutation of rue family citrus plant Citrus aurantium Linn. from generation to generation, be distributed in south China various places, there is cultivation on the ground such as Zhejiang, Jiangsu, Guangdong, Guizhou.CitrusaurantiumL.Var.amara Engl. sweet in the mouth, bitter in the mouth, property is put down, energy depressed liver-energy dispersing and QI regulating, stomach function regulating preventing or arresting vomiting.Containing Flavonoid substances such as neohesperidin, naringin, Hesperidins in its immaturity peel, in volatile oil, main component is limonene, linalool, dehydrolinalool, acetaminol etc.
At present spice is produced to the volatile oil that the research of CitrusaurantiumL.Var.amara Engl. mainly uses it to contain.CitrusaurantiumL.Var.amara Engl. extract as fragrance applications in the fields such as Nicotiana tabacum L..Meanwhile, research report in recent years, CitrusaurantiumL.Var.amara Engl. extract also has effect of Weight-reducing and lipid-lowering.CN201110317409 discloses the water extract of CitrusaurantiumL.Var.amara Engl. or alcohol extract preparing Weight-lossing hypolipemic medicine or preparation and has application in the medicine of lipase activity inhibition.And to the antioxidation of the flavonoid substances such as its Hesperidin contained, neohesperidin and the research of UV defencive function less.
The process of skin aging is a complicated biological phenomenon, is divided into natural aging and extrinsic aging (photoaging).Photoaging is mainly contacted or infringement accumulation [1-4] that the reason of life style produces with other environmental factorss by ultraviolet radiation.Skin aging shows as epidermis and hypodermal cell metabolic rate and reduces, atrophoderma [5], and it is flat that the fold at epidermal-dermal junction place becomes.In addition, the skin elasticity attenuating that collagen protein and elastin laminin synthesis reduce and cause also is an aspect of skin aging.
It is the key factor causing skin injury that smoking, ethanol, environmental pollution, malnutrition and ultraviolet (UV) expose.UV acts on the various photoactive substances in skin, can induce the oxygen-derived free radicals (ROS) produced based on OH-and lipid peroxide, destroy the antioxidant system of skin self, activities of antioxidant enzymes level is declined and causes lipid peroxide to pile up.The oxidative stress that UV causes also can cause a series of Cellular Signaling Transduction Mediated, activates the isogenic expression of downstream MMP by Nuclear Factor kappa B or transcription factor activator protein 1 (AP-1).After the uv irradiation, content obviously increases AP-I, and result in the content of MMP mRNA and express increase, ultraviolet aging skin lesion, appears in selective degradation extracellular matrix.Meanwhile, ultraviolet significantly can increase the expression of epidermis cell IL-1 α, thus causes more inflammatory factors as the expression of IL-6, IL-8, TNF-α, accelerates skin aging.
The research of the anti-aging and UV defencive function of the flavonoid substances such as Hesperidin, neohesperidin in CitrusaurantiumL.Var.amara Engl. extract of the present invention, provides the new method of UV resistance, anti-skin ageing.
Summary of the invention
The technical problem to be solved in the present invention is to provide New function and the radioprotective of CitrusaurantiumL.Var.amara Engl. extract, defying age and the application in skin care field thereof.
The present invention relates to the New function of CitrusaurantiumL.Var.amara Engl. extract.Find through experiment, CitrusaurantiumL.Var.amara Engl. extract significantly can reduce the injury of ultraviolet to cell, promotes the propagation of cell, promotes that fibroblast IV collagen mRNA ground is expressed simultaneously, suppresses elastase activity.
Therefore, CitrusaurantiumL.Var.amara Engl. extract can be applied to radioprotective, the skin care field of anti-skin aging.Described skin care item CitrusaurantiumL.Var.amara Engl. extract as sole active agent, also can contain other adjuvants and known skin care ingredient.Be nonacid after other adjuvants described and known skin care ingredient are in harmonious proportion.
Meanwhile, CitrusaurantiumL.Var.amara Engl. extract can be used as additive or adjuvant preparation have senile-resistant efficacy food, beverage and health product etc.
Used kit raw material of the present invention all commercially.
Beneficial effect of the present invention is: the invention provides the radioprotective of CitrusaurantiumL.Var.amara Engl. extract, antidotal New function, also provides its application in skin care field, has widened its function and application scope, for skin radioprotective and defying age provide new way.
Accompanying drawing explanation
By reading hereafter detailed description of the preferred embodiment, various other advantage and benefit will become cheer and bright for those of ordinary skill in the art.Accompanying drawing only for illustrating the object of preferred implementation, and does not think limitation of the present invention.In the accompanying drawings:
Fig. 1 CitrusaurantiumL.Var.amara Engl. extract promotes cell proliferation schematic diagram;
Fig. 2 CitrusaurantiumL.Var.amara Engl. extract promotes cell elasticity protein expression schematic diagram;
Fig. 3 CitrusaurantiumL.Var.amara Engl. extract suppresses elastoser and the active schematic diagram of matrix metal proteinase activity;
Fig. 4 CitrusaurantiumL.Var.amara Engl. extract reduces inflammatory factor secretion schematic diagram;
Fig. 5 CitrusaurantiumL.Var.amara Engl. extract reduces cyclobutane pyrimidine dimer (CPDs) and forms schematic diagram;
Fig. 6 CitrusaurantiumL.Var.amara Engl. extract reduces active oxygen and produces schematic diagram.
Detailed description of the invention
Experiment material:
Cell culture: cell category is that human embryonic fibroblast (ESF), immortalization epithelial cell (Hacat cell) are all purchased from consonance cellular resources center, Beijing.Condition of culture is DMEM culture medium (Gibco, the U.S.), and FBS containing 10% (Hyclone, Australia) and the antibacterium of 1% and the antibiotic (Invitrogen, the U.S.) of fungus, at 37 DEG C, CO
2concentration is the interior cultivation of incubator of 5%.
Prepared by CitrusaurantiumL.Var.amara Engl. extract: CitrusaurantiumL.Var.amara Engl. medical material is suitably pulverized, and takes 20g and is placed in hermetic container, and add 60% ethanol 200mL in 1: 10 ratio, supersound extraction 1h, is placed into room temperature, and filter, filtrate 50 DEG C of oven for drying, obtain CitrusaurantiumL.Var.amara Engl. extract dry powder.Extract dry powder takes appropriate extract dry powder, uses deionized water dissolving, filters for subsequent use.
Embodiment 1 Antisenility Experiment
1. promote cell proliferation
ESF cell adjustment cell concentration is 4 × 10
4/ ml, is inoculated in 24 orifice plates, 0.5ml/ hole, and namely 2 × 10
4individual/well.Cell culture spends the night after cell attachment, use the culture medium culturing 24h containing 1%FBS instead, cell is divided into 2 groups, i.e. control group (culture medium is added without CitrusaurantiumL.Var.amara Engl. extract), experimental group (culture medium adds the CitrusaurantiumL.Var.amara Engl. extract of variable concentrations).Experimental group CitrusaurantiumL.Var.amara Engl. extract adds concentration and is respectively: 10 μ g/ml, 50 μ g/ml, 100 μ g/ml.After 72h, removing culture medium, washes once with DPBS, adds the new culture fluid that 200ul contains the CCK-8 of 10%, and 37 DEG C of temperature bath about 1h, get supernatant in 96 orifice plates, uses microplate reader 450nm wavelength detecting supernatant OD value.
2. promote that cell elasticity albumen and collagen protein are expressed
Cell culture:
ESF cell is divided into 2 groups, i.e. control group (culture medium is added without CitrusaurantiumL.Var.amara Engl. extract), experimental group (culture medium adds the CitrusaurantiumL.Var.amara Engl. extract of variable concentrations).It is 10 μ g/ml that CitrusaurantiumL.Var.amara Engl. extract adds concentration, 20 μ g/ml, 50 μ g/ml.
RNA extracts and QPCR experiment
Experimental group cell is by after above-mentioned variable concentrations CitrusaurantiumL.Var.amara Engl. extract-treated 24h, control group cell and experimental group cell use RNeasyPlus Mini Kit (Qiagen, Germany) cell total rna is extracted, rna content uses nanodrop 2000 to detect quantitatively, get 1 μ g as template, SUPERSCRIPT III 1ST STRAND 50RT-PCR REACTIONS (Invitrogen, the U.S.) is used to carry out reverse transcription.After reverse transcription, dilution cDNA concentration is 10ng/ul.Q-PCR reaction system is cDNA template 1ul, forward and reverse primer each 0.5ul, Power SYBR Green PCR Master Mix (ABI, the U.S.) 10ul, adds water and mends to 20ul.Elastin laminin primer: GGTATCCCATCAAGGCCCC (SEQ ID NO.1), TTTCCCTGTGGTGTAGGGCA (SEQ ID NO.2); IV Collagen Type VI primer: CGCTTACAGCTTTTGGCTCG (SEQ ID NO.3), GACGGCGTAGGCTTCTTGAA (SEQID NO.4).
3. pair elastoser (HLE) and matrix metalloproteinase (MMP-1) Inhibition test
The inhibition test of CitrusaurantiumL.Var.amara Engl. to elastoser and matrix metalloproteinase uses cell free assay to carry out, CitrusaurantiumL.Var.amara Engl. extract uses elastase activity detection kit to carry out (Invitrogen to the inhibitory action of elastase activity, the U.S.), MMP-1 detection kit is used to carry out (Invitrogen, the U.S.) to the inhibitory action of matrix metalloproteinase MMP-1 enzymatic activity.CitrusaurantiumL.Var.amara Engl. extract concentrations is respectively: 0.1mg/ml, 0.5mg/ml, 1mg/ml.
Embodiment 2 radioprotective is tested
Hacat cell and ESF cell are with 4 × 10
4cells/well is inoculated in 12 orifice plates, or 2x10
5cells/well is inoculated in 6 orifice plates, prepares to carry out UVB radiation treatment when being cultured to cell fusion more than 80%.
1. inflammatory factor IL-1 α, IL-6 and TNF-a content detection
Cell is divided into 3 groups, namely control group is (without UVB process, culture medium without CitrusaurantiumL.Var.amara Engl. extract add), (the UVB process of UVB group, culture medium without CitrusaurantiumL.Var.amara Engl. extract add), UVB+ CitrusaurantiumL.Var.amara Engl. group (UVB process, culture medium interpolation variable concentrations CitrusaurantiumL.Var.amara Engl. extract).UVB+ CitrusaurantiumL.Var.amara Engl. group CitrusaurantiumL.Var.amara Engl. extract concentrations is respectively: 10 μ g/ml, 50 μ g/ml, 100 μ g/ml.
After cell inoculation 24h, cell sucks culture medium, adds PBS and cleans cell once, and removing PBS, puts into ultraviolet irradiation case and carry out UVB process.UVB irradiation bomb wavelength 280-320nm, crest is 312nm.UVB process Hacat cell irradiation dose is 20mJ/cm
2, process ESF cell irradiation dose is 60mJ/cm
2.Add complete medium and CitrusaurantiumL.Var.amara Engl. extract cultivation 24h after radiation treatment completes, cell culture supernatant is used for IL-1 content detection.
IL-1 alpha content end user IL-1 α/IL-1F1DuoSet ELISA kit (the R & D System of Hacat cell after UVB process in culture supernatant, the U.S.) detect, IL-6 content detection uses human IL-6DuoSet ELISA kit (R & D System, the U.S.) carry out, TNF-a content detection uses human TNF-a DuoSet ELISA kit (R & D System, the U.S.) carry out, the same description of method.Detect protein content to correct result, determining the protein quantity uses BCA determination of protein concentration test kit (the green skies, China) to detect simultaneously.
2.CPD detects
The process of Hacat cell is the same, uses CitrusaurantiumL.Var.amara Engl. extract concentrations to be 10 μ g/ml, 20 μ g/ml, 50 μ g/ml.UVB dosage is used to be 5mJ/cm
2.After process, cell uses DNA extraction kit (QIAGEN, Germany) carry out total DNA extraction after, through quantitative, adjustment DNA concentration is 0.2ng/ μ l, add and use Protamine sulfates. (sigma in advance, the U.S.) bed board 96 hole ELISA Plate on, use ELISA method carry out CPD content detection.Described CPD antibody is Japanese Cosmobio Products, and two resist the rabbit anti-mouse IgG for Invitrogen company Biotin labelling.
3. active oxygen (ROS) horizontal detection
Hacat cell and ESF cell are through UVB process, and method detects with inflammatory factor.ROS detection kit (the green skies, China) is used to carry out the detection of intracellular reactive oxygen species generation (ROS) level.Fluorescence signal uses fluorescence analyser (Thermo Scientific, the U.S.) to carry out, and excitation wavelength is 485nm, and emission wavelength is 538nm.Detect protein content to correct result, determining the protein quantity uses BCA determination of protein concentration test kit (the green skies, China) to detect simultaneously.
Experimental result and analysis
1, Antisenility Experiment: as shown in Figure 1, CitrusaurantiumL.Var.amara Engl. can promote ESF cell proliferation, and facilitation and concentration are proportionate (Fig. 1).
The E S F cell total rna extracted after CitrusaurantiumL.Var.amara Engl. process carries out QPCR, and result is shown as the expression that CitrusaurantiumL.Var.amara Engl. can promote elastin laminin, and is dependence (Fig. 2) with concentration.As shown in Figure 3, elastin laminin is the main substrate of elastoser (HLE), suppresses elastase activity better can protect elastin laminin, can detect the inhibitory action of biological active matter confrontation elastoser.Elastin laminin is labeled fluorophor and quenching group, does not now fluoresce.After elastin laminin is by elastase degradation, quenching group dissociates, and substrate sends fluorescence, can judge enzymatic activity according to fluorescence intensity, adds activity inhibitor and can judge the inhibition strength of inhibitor to enzymatic activity.Calculate with unrestraint rate during without inhibitor, when CitrusaurantiumL.Var.amara Engl. extract concentrations is 1mg/ml, can about 70% be reached to the suppression ratio of HLE.The Cleaning Principle that MMP-1 activity suppresses is with the detection of HLE, and CitrusaurantiumL.Var.amara Engl. extract also has stronger suppression ratio to MMP-1 activity, reaches about 40% (Fig. 3) when 1mg/ml concentration
2, radioprotective experiment:
As shown in Figure 4, UVR process can cause the Hacat cell proinflammatory factor IL-1 α, IL-6 and TNF-a to express and increase, thus cause the activation of other inflammatory factors, after using UVB process Hacat cell, IL-1 alpha expression significantly increases, after using CitrusaurantiumL.Var.amara Engl. extract-treated, IL-1 α, IL-6 and TNF-a express and obviously reduce (Fig. 4).Show that CitrusaurantiumL.Var.amara Engl. extract can suppress the inflammatory reaction caused by UVB to shield to epithelial cell.
As shown in Figure 5, the photoproduct that ultraviolet causes DNA Damage to be formed mainly contains two types, i.e. cyclobutane pyrimidine dimer (CPDs) and 6-4 photoproduct (6-4) PPs, and wherein CPDs accounts for 80%.CPD (Cyclobutane Pyrimidine Dimer) is that the pyrimidine bases that in DNA molecular, same chain is adjacent form the dimer connecting into Tetramethylene. structure with covalent bond, affects the double-spiral structure of DNA, makes to copy and be obstructed with functional transcription.Whether affect the generation of CPD for detecting CitrusaurantiumL.Var.amara Engl., this experiment uses 5mJ/cm
2uVB process Hacat cell, result display UVB process significantly can increase Hacat cell CPD content, and add CitrusaurantiumL.Var.amara Engl. extract, CPD content significantly reduces (Fig. 5).
As shown in Figure 6, the cell injury major part reason that UV causes is the generation due to active oxygen.For the impact that research CitrusaurantiumL.Var.amara Engl. produces active oxygen, UVB is used to carry out treatment with irradiation to Hacat and ESF cell, result display UVB can significantly improve the ROS level of Hacat and ESF cell, uses CitrusaurantiumL.Var.amara Engl. process cell, can reduce the ROS level (Fig. 6) that UVB causes.
Because CitrusaurantiumL.Var.amara Engl. extract can effectively prevent ROS from producing, therefore it can also to be applied as additive or adjuvant in preparation effect is antidotal food, beverage and health product etc.
To sum up, CitrusaurantiumL.Var.amara Engl. extract significantly can promote cell proliferation, promote that fibroblast IV collagen mRNA ground is expressed, suppress elastoser and matrix metal proteinase activity, reduce ultraviolet to the injury of cell simultaneously, reduce reactive oxygen species, reduce inflammatory factor secretion, thus radioprotective, aging-resistant function are played to skin.
Because CitrusaurantiumL.Var.amara Engl. extract has above-mentioned New function, therefore can as the effective ingredient of sun-proof and anti-skin aging skin-protection product, namely it can be used as effective ingredient unique in skin-protection product, be allocated as aqueous solution, also can merge with existing adjuvant and other nonacid skin care ingredient and allocate, being not limited to single product form, can be facial cream, surfactant, sun screen, emulsion, facial film etc.
In the above-described embodiments, preferred forms of the present invention is described, obviously, under inventive concept of the present invention, still can make a lot of change.At this, should illustrate, any change made under inventive concept of the present invention all will fall within the scope of protection of the present invention.
Claims (5)
1. a CitrusaurantiumL.Var.amara Engl. extract is preparing the application in skin-protection product.
2. the application of CitrusaurantiumL.Var.amara Engl. extract in preparation radioprotective, antisenility skin care product.
3. CitrusaurantiumL.Var.amara Engl. extract is application in antidotal food, beverage and health product as additive or adjuvant in preparation effect.
4. the application as described in claim 1-3, the function of CitrusaurantiumL.Var.amara Engl. extract is radioprotective, defying age, promote cell proliferation, promote that fibroblast IV collagen mRNA ground is expressed, suppress elastoser and matrix metal proteinase activity, reduce ultraviolet to the injury of cell simultaneously, reduce reactive oxygen species, reduce inflammatory factor secretion.
5. apply as claimed in claim 1, its skin-protection product be applied to can be facial cream, surfactant, sun screen, emulsion, facial film.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105662941A (en) * | 2016-01-11 | 2016-06-15 | 无限极（中国）有限公司 | Immature bitter orange extract product and preparation method thereof and application of immature bitter orange extract product for delaying skin aging |
| CN107737059A (en) * | 2017-12-04 | 2018-02-27 | 刘晓冰 | A kind of centella whitening lotion |
| CN111346142A (en) * | 2018-12-22 | 2020-06-30 | 大江生医股份有限公司 | Application of seville orange flower extract in preparation of composition for improving anti-saccharification activity, inhibiting fat accumulation and promoting lipolysis |
| CN112190508A (en) * | 2020-08-18 | 2021-01-08 | 湖南神农国油生态农业发展有限公司 | Compound acne-removing mask and preparation method thereof |
| CN116747262A (en) * | 2023-08-14 | 2023-09-15 | 云南英格生物技术有限公司 | Substituted flower active substance and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000096050A (en) * | 1998-09-25 | 2000-04-04 | Shiseido Co Ltd | Promoter for production of hyaluronic acid |
| FR2825627A1 (en) * | 2001-06-06 | 2002-12-13 | Gattefosse Ets Sa | Citrus blossom bud extract with high citroflavonoid content, useful in cosmetic or pharmaceutical compositions, e.g. for protecting sensitive skin, treating acne, rosaceae or combating wrinkles |
| CN101422555A (en) * | 2008-12-23 | 2009-05-06 | 武汉名实生物医药科技有限责任公司 | Preparation capable of defaecating, adjusting qi and blood, improving internal secretion and preventing senescence |
-
2014
- 2014-11-05 CN CN201410613299.8A patent/CN104434622A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000096050A (en) * | 1998-09-25 | 2000-04-04 | Shiseido Co Ltd | Promoter for production of hyaluronic acid |
| FR2825627A1 (en) * | 2001-06-06 | 2002-12-13 | Gattefosse Ets Sa | Citrus blossom bud extract with high citroflavonoid content, useful in cosmetic or pharmaceutical compositions, e.g. for protecting sensitive skin, treating acne, rosaceae or combating wrinkles |
| CN101422555A (en) * | 2008-12-23 | 2009-05-06 | 武汉名实生物医药科技有限责任公司 | Preparation capable of defaecating, adjusting qi and blood, improving internal secretion and preventing senescence |
Non-Patent Citations (1)
| Title |
|---|
| 陈丹 等: "代代的研究进展", 《福建中医学院学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105662941A (en) * | 2016-01-11 | 2016-06-15 | 无限极（中国）有限公司 | Immature bitter orange extract product and preparation method thereof and application of immature bitter orange extract product for delaying skin aging |
| CN107737059A (en) * | 2017-12-04 | 2018-02-27 | 刘晓冰 | A kind of centella whitening lotion |
| CN111346142A (en) * | 2018-12-22 | 2020-06-30 | 大江生医股份有限公司 | Application of seville orange flower extract in preparation of composition for improving anti-saccharification activity, inhibiting fat accumulation and promoting lipolysis |
| CN112190508A (en) * | 2020-08-18 | 2021-01-08 | 湖南神农国油生态农业发展有限公司 | Compound acne-removing mask and preparation method thereof |
| CN116747262A (en) * | 2023-08-14 | 2023-09-15 | 云南英格生物技术有限公司 | Substituted flower active substance and preparation method and application thereof |
| CN116747262B (en) * | 2023-08-14 | 2023-11-10 | 云南英格生物技术有限公司 | Substituted flower active substance and preparation method and application thereof |
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Application publication date: 20150325 |