CN104430849A - Multi-bacteria fermented skim camel milk for resisting diabetes and production method of multi-bacteria fermented skim camel milk - Google Patents

Multi-bacteria fermented skim camel milk for resisting diabetes and production method of multi-bacteria fermented skim camel milk Download PDF

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CN104430849A
CN104430849A CN201410751903.3A CN201410751903A CN104430849A CN 104430849 A CN104430849 A CN 104430849A CN 201410751903 A CN201410751903 A CN 201410751903A CN 104430849 A CN104430849 A CN 104430849A
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leavening
lactobacillus
freeze
dried powder
degreasing
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CN104430849B (en
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新华·那比
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Xinjiang Wangyuan Camel Milk Industrial Co ltd
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Xinjiang Medical University
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Abstract

The invention relates to the technical field of fermented dairy products, and discloses multi-bacteria fermented skim camel milk for resisting diabetes and a production method of the multi-bacteria fermented skim camel milk. The multi-bacteria fermented skim camel milk is prepared from a lactobacillus helveticus leavening agent, a lactobacillus plantarum leavening agent, a lactobacillus paracasei leavening agent, a lactobacillus paracasei hard subspecies leavening agent, a lactobacillus mucosae leavening agent, a Harbin lactobacillus leavening agent, a lactobacillus hilgardii leavening agent, a lactobacillus pentosus leavening agent, a lactobacillus rhamnosus leavening agent, a lactococcus lactis leavening agent, an issatchenkia orientalis leavening agent, a kluyveromyces marxianus leavening agent, a pichia membranefaciens leavening agent and an ethanol candida leavening agent, which are added to the skim camel milk and are fermented. The multi-bacteria fermented skim camel milk is good in taste, the production method is easy to operate and simple in operation method; the obtained multi-bacteria fermented skim camel milk is abundant in nutrient, contains the components such as probiotics, immunomdodulating peptide, anti-oxidative peptide and the like, and has antidiabetic, anti-inflammation and antioxidant effects; and the physique and the overall vitality of people can be enhanced.

Description

For antidiabetic mixed culture fermentation degreasing camel breast and production method thereof
Technical field
the present invention relates to fermented milk products technical field, is a kind of for antidiabetic mixed culture fermentation degreasing camel breast and production method thereof.
Background technology
in the U.S., there are more than 2,600 ten thousand people by the puzzlement of diabetes, wherein 90% to 95% is diabetes B, and two large abnormal conditions can cause diabetes B below: (1) insulin resistance, and this can cause insulin to suppress liver glucose to generate and stimulate peripheral tissues to glucose uptake capability deteriorates; (2) insulin secretion Progressive symmetric erythrokeratodermia is impaired.The World Health Organization and IDF give a warning to the whole world, the current whole world has 1.71 hundred million people's having diabetes at least, may double to the year two thousand thirty number, reach 3.66 hundred million people, become the country that diabetic's number is maximum in the world, China is faced with diabetes and is very popular the huge challenge brought.In recent years under this situation, receive the extensive concern of people with the fermented milk products that " functional food " is famous.
the OHA applied clinically mainly contains 4 large classes, be respectively, Drugs Promoting Insulin Secretion, biguanides, alpha-glucosidase restrainer, thiazolidinediones, these medicines can alleviate insulin resistance or promote insulin secretion, use effectively in early days in the course of disease, insulin is then all effective to all stages of disease, and often finally need with insulin to reach the object controlling blood sugar, there is dosage according to patience, inconvenience, expensive, and, insulin injection easily causes glucose too low, suffers a shock time serious.
Summary of the invention
the invention provides a kind of for antidiabetic mixed culture fermentation degreasing camel breast and production method thereof, overcome the deficiency of above-mentioned prior art, because it is nutritious, containing compositions such as profitable probliotics, immunomodulatory peptides, anti-oxidation peptides, there is antidiabetic effect.
one of technical scheme of the present invention is realized by following measures: a kind of for antidiabetic mixed culture fermentation degreasing camel breast, Lactobacillus helveticus (Lactobacillus helveticus) leavening is added to degreasing camel Ruzhong, Lactobacillus plantarum (Lactobacillus plantarum) leavening, lactobacillus paraceasi (Lactobacillus paracasei) leavening, the tough and tensile subspecies of lactobacillus paraceasi class (Lactobacillus paracasei subsp.tolerans) leavening, Lactobacillus mucosae (Lactobacillus mucosae) leavening, Harbin lactobacillus (Lactobacillus harbinensis) leavening, Lactobacillus hilgardii (Lactobacillus hilgardii) leavening, Lactobacillus pentosus (Lactobacillus pentosus) leavening, Lactobacillus rhamnosus (Lactobacillus rhamnosus) leavening, Lactococcus lactis (Lactococcus lactis) leavening, Issatchenkia orientalis (Issatchenkia orientalis) leavening, kluyveromyces marxianus (Kluyveromyces marxianus) leavening, Pichia membranaefaciens (Pichia membranifaciens) leavening and ethanol Candida (Candida ethanolica) ferment-fermented after obtain,
wherein, the weight ratio between the weight sum of Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening and degreasing camel breast is 1:15 to 25;
wherein, Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, weight ratio between Pichia membranaefaciens leavening and ethanol Candida leavening is that 0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 is to 1.0.
here is the further optimization and/or improvements to one of foregoing invention technical scheme:
above-mentioned mixed culture fermentation degreasing camel breast obtains as follows: the first step, degreasing camel breast through pasteurize be cooled to 30 DEG C for subsequent use to about 40 DEG C, second step, the Lactobacillus helveticus leavening of requirement is added to the degreasing camel Ruzhong after pasteurize, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening, at 35 DEG C to 40 DEG C, mixed culture fermentation 8 is little obtained mixed culture fermentation degreasing camel breast up to 12 hours.
the condition that above-mentioned degreasing camel breast carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 5min to 10min carries out sterilization, or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous, or/and, by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening add the camel of the degreasing after pasteurize Ruzhong respectively to, or by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening admixed together after add to the degreasing after pasteurize camel Ruzhong.
< TranNum b = "69" > the Swiss lactobacillus starter or plant lactobacillus starter or vice lactobacillus casei culture or vice lactobacillus casei tenacity subspecies provides or mucosal lactobacillus starter or Harbin lactobacillus starter or bush's lactobacillus starter or starter or rat lee lactobacillus pentose sugar lactobacillus starter or lactic acid milk coccus culture yeast or east issa provides Max crewe yeast starter or film pellicle of candida yeast pichia yeast or ethanol according to the following weight ratio to get raw materials, the Swiss lactobacillus producing or plant lactobacillus producing or producing or deputy vice lactobacillus casei lactobacillus casei tenacity subspecies producing or mucosal lactobacillus producing or Harbin lactobacillus producing or bush's lactobacillus producing or producing or rat lee lactobacillus pentose sugar lactobacillus producing lactic acid milk aureus producing or Oriental issa producing yeast or Max crewe producing or film pellicle pichia yeast candida producing and producing or ethanol degreasing than the weight of the camel milk is 1:100 to 200;< / b >
wherein, the concrete preparation method of Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is: first degreasing camel breast is carried out pasteurize, then degreasing camel breast is cooled to 30 DEG C to 40 DEG C, then the Lactobacillus helveticus freeze-dried powder of aequum or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder are joined the degreasing camel Ruzhong of aequum, Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or the tough and tensile subspecies leavening of lactobacillus paraceasi class or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is obtained 35 DEG C to 40 DEG C condition bottom fermentations 18 are little up to 24 hours.
the condition that above-mentioned degreasing camel breast carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 30min to 60min carries out sterilization; Or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous.
two of technical scheme of the present invention is realized by following measures: the production method of a kind of mixed culture fermentation degreasing camel breast, carries out in the steps below: the first step, degreasing camel breast through pasteurize be cooled to 30 DEG C for subsequent use to about 40 DEG C, second step, the Lactobacillus helveticus leavening of requirement is added to the degreasing camel Ruzhong after pasteurize, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening, at 35 DEG C to 40 DEG C, mixed culture fermentation 8 is little obtained mixed culture fermentation degreasing camel breast up to 12 hours.
here is the further optimization and/or improvements to foregoing invention technical scheme two:
the condition that above-mentioned degreasing camel breast carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 5min to 10min carries out sterilization, or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous, or/and, by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening add the camel of the degreasing after pasteurize Ruzhong respectively to, or by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening admixed together after add to the degreasing after pasteurize camel Ruzhong.
< TranNum b = "81" > the Swiss lactobacillus starter or plant lactobacillus starter or vice lactobacillus casei culture or vice lactobacillus casei tenacity subspecies provides or mucosal lactobacillus starter or Harbin lactobacillus starter or bush's lactobacillus starter or starter or rat lee lactobacillus pentose sugar lactobacillus starter or lactic acid milk coccus culture yeast or east issa provides Max crewe yeast starter or film pellicle of candida yeast pichia yeast or ethanol according to the following weight ratio to get raw materials, the Swiss lactobacillus producing or plant lactobacillus producing or producing or deputy vice lactobacillus casei lactobacillus casei tenacity subspecies producing or mucosal lactobacillus producing or Harbin lactobacillus producing or bush's lactobacillus producing or producing or rat lee lactobacillus pentose sugar lactobacillus producing lactic acid milk aureus producing or Oriental issa producing yeast or Max crewe producing or film pellicle pichia yeast candida producing and producing or ethanol degreasing than the weight of the camel milk is 1:100 to 200;< / b >
wherein, the concrete preparation method of Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is: first degreasing camel breast is carried out pasteurize, then degreasing camel breast is cooled to 30 DEG C to 40 DEG C, then the Lactobacillus helveticus freeze-dried powder of aequum or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder are joined the degreasing camel Ruzhong of aequum, Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or the tough and tensile subspecies leavening of lactobacillus paraceasi class or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is obtained 35 DEG C to 40 DEG C condition bottom fermentations 18 are little up to 24 hours.
the condition that above-mentioned degreasing camel breast carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 30min to 60min carries out sterilization; Or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous.
the present invention is easy to operation, production method is simple, mouthfeel is good, and gained mixed culture fermentation degreasing camel breast is nutritious, containing compositions such as profitable probliotics, immunomodulatory peptides, anti-oxidation peptides, there is anti-diabetic, anti-inflammatory, the effect such as anti-oxidant, physique and the overall activity of people can be strengthened.
Accompanying drawing explanation
accompanying drawing 1 is the enlarged drawing of Normal group pancreas × 100 times in the present invention.
accompanying drawing 2 is the enlarged drawing of diabetic model group × 100 times in the present invention.
accompanying drawing 3 is the enlarged drawing of Ge Lieting group × 100, Chinese and Western of the present invention times.
accompanying drawing 4 is the enlarged drawing of fermented camel milk low dose group × 100 times in the present invention.
accompanying drawing 5 is the enlarged drawing of dosage group × 100 times in fermented camel milk in the present invention.
accompanying drawing 6 is the enlarged drawing of heavy dose of group × 100 of fermented camel milk times in the present invention.
Detailed description of the invention
the present invention by the restriction of following embodiment, can not determine concrete embodiment according to technical scheme of the present invention and actual conditions.Unless stated otherwise, the % in the present invention is mass percent; Unless stated otherwise, preparation process is carried out under normal temperature, atmospheric pressure state; Unless stated otherwise, the reagent adopted in the present invention, method and apparatus are the art conventional reagent, method and apparatus; Unless stated otherwise, the culture medium adopted in the present invention and experimental condition are the art conventional medium and experimental condition; Unless stated otherwise, in the present invention, agents useful for same is commercial; Unless stated otherwise, the water in the present invention is deionized water; Unless stated otherwise, the solution in the present invention is the aqueous solution that solvent is water, and such as, if illustrate, hydrochloric acid solution is aqueous hydrochloric acid solution.
below in conjunction with embodiment, the invention will be further described:
embodiment 1, this is used for antidiabetic mixed culture fermentation degreasing camel breast, Lactobacillus helveticus (Lactobacillus helveticus) leavening is added to degreasing camel Ruzhong, Lactobacillus plantarum (Lactobacillus plantarum) leavening, lactobacillus paraceasi (Lactobacillus paracasei) leavening, the tough and tensile subspecies of lactobacillus paraceasi class (Lactobacillus paracasei subsp.tolerans) leavening, Lactobacillus mucosae (Lactobacillus mucosae) leavening, Harbin lactobacillus (Lactobacillus harbinensis) leavening, Lactobacillus hilgardii (Lactobacillus hilgardii) leavening, Lactobacillus pentosus (Lactobacillus pentosus) leavening, Lactobacillus rhamnosus (Lactobacillus rhamnosus) leavening, Lactococcus lactis (Lactococcus lactis) leavening, Issatchenkia orientalis (Issatchenkia orientalis) leavening, kluyveromyces marxianus (Kluyveromyces marxianus) leavening, Pichia membranaefaciens (Pichia membranifaciens) leavening and ethanol Candida (Candida ethanolica) is ferment-fermented obtains, each leavening needs to carry out vitality test to each leavening before being inoculated in degreasing camel breast, adopt resazurin reduction experimental method: add 1ml leavening and 0.005% resazurin solution 1ml in 9ml degreasing camel Ruzhong, in the constant incubator of 37 DEG C, cultivate 35min, observe solution and whether fade completely.(resazurin adds normal Ruzhong, and breast in cyanic colours, if there is starter culture in Ruzhong, can make resazurin reduction, by cyanic colours → purple → redness → colourless.The vigor of leavening is judged according to resazurin reduction time speed.Get 1ml leavening to add in 9ml sterile milk test tube, then add resazurin standard liquid 1ml.Simultaneously not with the control tube of leavening.Start after test tube being placed in 37 DEG C of water bath heat preservation 35min to observe.Thereafter every 5min observes once result.Rose pink is deoxidization terminal, using the time that terminal occurs as the index evaluating the activity of starter.Note: the vigor of leavening of resazurin of reducing in 35min is very strong; The activity of starter of resazurin of reducing in 50min is poor, but can use; The activity of starter of 50 ~ 60min reduction resazurin is very weak, should not use).
wherein, the weight ratio between the weight sum of Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening and degreasing camel breast is 1:15 to 25;
wherein, Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, weight ratio between Pichia membranaefaciens leavening and ethanol Candida leavening is that 0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 is to 1.0.
embodiment 2, this is used for antidiabetic mixed culture fermentation degreasing camel breast, Lactobacillus helveticus (Lactobacillus helveticus) leavening is added to degreasing camel Ruzhong, Lactobacillus plantarum (Lactobacillus plantarum) leavening, lactobacillus paraceasi (Lactobacillus paracasei) leavening, the tough and tensile subspecies of lactobacillus paraceasi class (Lactobacillus paracasei subsp.tolerans) leavening, Lactobacillus mucosae (Lactobacillus mucosae) leavening, Harbin lactobacillus (Lactobacillus harbinensis) leavening, Lactobacillus hilgardii (Lactobacillus hilgardii) leavening, Lactobacillus pentosus (Lactobacillus pentosus) leavening, Lactobacillus rhamnosus (Lactobacillus rhamnosus) leavening, Lactococcus lactis (Lactococcus lactis) leavening, Issatchenkia orientalis (Issatchenkia orientalis) leavening, kluyveromyces marxianus (Kluyveromyces marxianus) leavening, Pichia membranaefaciens (Pichia membranifaciens) leavening and ethanol Candida (Candida ethanolica) is ferment-fermented obtains,
wherein, the weight ratio between the weight sum of Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening and degreasing camel breast is 1:15 or 25;
wherein, Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, weight ratio between Pichia membranaefaciens leavening and ethanol Candida leavening is 0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0:0.8 or 1.0.
embodiment 3, preferred as embodiment 1 and embodiment 2, this mixed culture fermentation degreasing camel breast obtains as follows: the first step, degreasing camel breast through pasteurize be cooled to 30 DEG C for subsequent use to about 40 DEG C, second step, the Lactobacillus helveticus leavening of requirement is added to the degreasing camel Ruzhong after pasteurize, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening, at 35 DEG C to 40 DEG C, mixed culture fermentation 8 is little obtained mixed culture fermentation degreasing camel breast up to 12 hours.
embodiment 4, preferred as embodiment 3, the condition that degreasing camel breast carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 5min to 10min carries out sterilization, or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous, or/and, by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening add the camel of the degreasing after pasteurize Ruzhong respectively to, or by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening admixed together after add to the degreasing after pasteurize camel Ruzhong.
embodiment 5, preferred as above-described embodiment, Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening obtain by following raw material weight ratio, the weight ratio of Lactobacillus helveticus freeze-dried powder or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder and degreasing camel breast is 1:100 to 200,
wherein, the concrete preparation method of Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is: first degreasing camel breast is carried out pasteurize, then degreasing camel breast is cooled to 30 DEG C to 40 DEG C, then the Lactobacillus helveticus freeze-dried powder of aequum or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder are joined the degreasing camel Ruzhong of aequum, Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or the tough and tensile subspecies leavening of lactobacillus paraceasi class or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is obtained 35 DEG C to 40 DEG C condition bottom fermentations 18 are little up to 24 hours.
embodiment 6, preferred as embodiment 5, the condition that degreasing camel breast carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 30min to 60min carries out sterilization; Or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous.
according to the analysis of health care composition of the above embodiment of the present invention gained mixed culture fermentation of the present invention degreasing camel breast and pharmacodynamics test as follows:
following test data is the average data according to the milk test of the above embodiment of the present invention gained mixed culture fermentation degreasing camel of the present invention.
1, the analysis of the health care composition of mixed culture fermentation degreasing camel breast of the present invention.
1.1, nutritional labeling
1.1.1, detection method and foundation
protein kjeldahl apparatus measures, and detects foundation: GB/T5413.1.3-1997;
lactose is pressed GB/T5413.5-1997 and is measured;
mineral matter Avanta-PM type atomic absorption spectrophotometer, the content of zinc is measured by GB/ T5009.14-2003, the content of iron and magnesium is measured by GB/T5009.90-2003, by GB/T5009.91,93-2003 measures the content of calcium and selenium, measure the content of potassium and sodium by GB/ T5009.91-2003, measure the content of phosphorus by GB/T5413.22-1997;
amino acids is pressed GB/T5009-2003 with automatic amino acid analyzer and is measured;
vitamins 1525 pump series of high efficiency liquid chromatographs, measure ascorbic content by GB/T6195-1986; The content of vitamin ae is measured by GB/T5009.82-2003.
saccharomycete viable count counts, and measures by GB 4789.15-2010;
viable lactic acid bacteria counting number, measures by GB 4789.35-2010;
the mensuration of acidity, measures by GB 5413.34-2010;
pH value measures, and adopts OAKTON PH instrument to measure.
1.1.2, result
1.1.2.1, total protein and 17 seed amino acid assays
measurement result shows: multiple nutritional components is rich in mixed culture fermentation degreasing camel Ruzhong of the present invention, total protein and amino acid content enrich, total protein content is 4.11% ± 0.33%, total amino acid content is 3.92 ± 0.45%, amino acid (containing the histidine that infant is required) wherein containing 8 kinds of needed by human, content is 1.75 ± 0.02%, close with human milk (43.0%).In each amino acid, be the highest with the content of glutamic acid, content is 0.81 ± 0.07%, and be secondly proline, content is 0.42 ± 0.06%.In addition, leucine (0.38% ± 0.05), lysine (0.32 ± 0.04%), aspartic acid (0.26% ± 0.02%), valine (0.24 ± 0.02%), isoleucine (0.21 ± 0.03%) content are also higher, and measurement result is in table 1.Leucine and isoleucine and valine are all branched-chain amino acids, they cooperation can repair muscle together, control blood sugar, and provide energy to bodily tissue, and the content of the mixed culture fermentation degreasing newborn leucine of camel obtained according to the above embodiment of the present invention and isoleucine and valine is all higher, therefore, also can find out thus, blood sugar can be controlled to a certain extent according to the mixed culture fermentation degreasing camel breast that the above embodiment of the present invention obtains, useful to diabetes patient.
1.1.2.2, mixed culture fermentation degreasing camel Ruzhong mineral matter composition of the present invention and content
mixed culture fermentation degreasing camel Ruzhong of the present invention content of mineral substances enriches, wherein the highest to the Determination of Potassium that human body is useful, be 147.96 ± 0.68mg/100g, secondly be calcium, content is 133.21 ± 6.46mg/100g, magnesium 9.37 ± 0.26mg/100g, zinc is 6.4 ± 3.24mg/100g, and to also have selenium to detect in mixed culture fermentation degreasing of the present invention camel Ruzhong be 0.07 ± 0.03 μ g.The results are shown in Table 2.
1.1.2.3, mixed culture fermentation degreasing camel Ruzhong vitamin composition of the present invention and content
in the main vitamins content of mixed culture fermentation degreasing of the present invention camel Ruzhong, vitamin C and content of vitamin E higher, the results are shown in Table 3.
2, probio
growth clump count: lactic acid bacteria adopts dilution overlapping plates cultivation, saccharomycete adopts dilution plate cultivation counting bacterium colony, the results are shown in Table 4.
mixed culture fermentation degreasing camel breast of the present invention, be rich in 10 kinds of lactic acid bacterias to be respectively: Lactobacillus helveticus (Lactobacillus helveticus) Lactobacillus plantarum (Lactobacillus plantarum), lactobacillus paraceasi (Lactobacillus paracasei), the tough and tensile subspecies of lactobacillus paraceasi class (Lactobacillus paracasei subsp.tolerans), Lactobacillus mucosae (Lactobacillus mucosae), Harbin lactobacillus (Lactobacillus harbinensis), Lactobacillus hilgardii (Lactobacillus hilgardii), Lactobacillus pentosus Lactobacillus pentosus, Lactobacillus rhamnosus (Lactobacillus rhamnosus), Lactococcus lactis (Lactococcus lactis), 4 Yeasts are respectively: kluyveromyces marxianus (Kluyveromyces marxianus), Pichia membranaefaciens (Pichia membranifaciens), ethanol Candida (Candida ethanolica), Issatchenkia orientalis (Issatchenkia orientalis).
result: mixed culture fermentation degreasing of the present invention camel breast is refrigerated in 4 DEG C of refrigerators preservation is after two weeks, recording viable count of lactobacillus is 8.63 × 10 8 cFU/ml, saccharomycete viable count is 1.87 × 10 6 cFU/ml.Find the viable count of lactic acid bacteria, significantly increase (p<0.05), saccharomycete viable count reduces to some extent, but difference is not remarkable.The results are shown in Table 4, comprehensive explanation mixed culture fermentation degreasing camel of the present invention breast is in During Cold Storage, and there is synbiosis between lactic acid bacteria saccharomycete, this synbiosis plays a role for the viable count maintaining fermented camel milk.
3, to the acidity of mixed culture fermentation degreasing of the present invention camel breast ( . t) and pH value measure, the results are shown in Table 5.
result: mixed culture fermentation degreasing of the present invention camel breast is refrigerated in 4 DEG C of refrigerators preservation is after two weeks, its pH value declines to some extent, illustrates that lactic acid bacteria continues fermentation generation lactic acid at During Cold Storage; The newborn pH value of preservation after fermentation in two weeks camel is 4.31, and total acidity is 85.6 . T。
4, mixed culture fermentation degreasing camel newborn adjusting blood lipid, antidiabetic effect of the present invention
4.1, materials and methods
4.1.1, animal used as test and reagent
healthy Wistar male rat 80, body weight 180 ± 20g, purchased from Xinjiang Medicine University's Experimental Animal Center (quality certification number: SCXK (newly) 2011-0004).Cholesterol (analyzing pure), sodium taurocholate is all purchased from Beijing extensive and profound in meaning star biotechnology responsibility Co., Ltd, streptozotocin (STZ, lot number: SLBH0076V, specification: 1g) be purchased from sigma company of the U.S., yellow Jackets (lot number: 1127H037, specification 5g, ) be purchased from Merck KGaA company, phosphoric acid Xi Gelieting sheet (lot number: V2361, specification: 100mg) purchased from Australian Merck Sharp & Dohme company, citric acid, trisodium citrate is all purchased from Tianjin Sheng Ao chemical reagent Co., Ltd, paraformaldehyde (lot number: 20140422, specification: 500ml) be purchased from Beijing Solarbio company, glucagon-like-peptide-1 ELISA kit (lot number: 2473256) be purchased from U.S.A. EMD Millipore Corporation, C peptide ELISA kit (lot number: C0520620164), glycosylated hemoglobin ELISA kit (lot number: A2819697) is all purchased from Wuhan Sino-American Biotechnology Company.
phosphoric acid Xi Gelieting sheet is the DPP-4 inhibitor that first approval is used for the treatment of diabetes B, β Apoptosis can be suppressed, promote β cell neogenesis, increase diabetes B patient β cell quantity, obvious reduction patient blood glucose, and significant blood sugar reducing function is still had to the patient that sulfa drugs lost efficacy.Therefore, the present invention adopts phosphoric acid Xi Gelieting sheet as team leader's medicine.
below in experiment, by newborn referred to as fermented camel milk for the mixed culture fermentation degreasing camel obtained according to the above embodiment of the present invention.
4.1.2, the foundation of male Wistar rat diabetes model
healthy Wistar male rat 80, body weight 180 ± 20g, feeding environment illumination 12h/d, room temperature 21 ± 2 DEG C, humidity 45-55%.After adaptability raises 3 days, randomly draw 10 rats and do Normal group, give common standard rat feed and feed, remain 70 modeling group rats and give high glucose and high fat forage feed, freely intake.Raise 8 weeks, weigh weekly once.At 8th weekend, Rat Fast can't help water 12h, the rat disposable celiac injection 0.1molL of Normal group -1 , PH4.4 trisodium citrate-citrate buffer solution, all the other modeling group rat disposable celiacs injection STZ 30mgkg -1 (STZ is dissolved in 0.1molL -1 , in PH4.4 trisodium citrate-citrate buffer solution).After 3 days, fasting 12h, tail point gets blood, measures fasting blood-glucose (FBG) by accurate blood glucose meter.If FBG is more than or equal to 11.1mmolL -1 fix tentatively as diabetes become mould rat, injection STZ solution 1 week, 2 weeks, fasting 12h respectively, surveys fasting blood-glucose, 3 FBG≤11.1mmolL -1 prove model stability, modeling success.
4.1.3, animal grouping and intervention
in the rat of 70 modelings, 10 do not reach into mould standard and are rejected, and by successful for modeling 60 rats, by the balanced random packet 5 groups of blood sugar, are respectively: diabetic model group, positive drug Xi Gelieting group (30mgkg -1 ), fermented camel milk high dose group (10 8 + 10 6 cFUmL -1 ), dosage group (10 in fermented camel milk 7 + 10 5 cFUmL -1 ), fermented camel milk low dose group (10 6 + 10 4 cFUmL -1 ), get with batch normal rat as Normal group, set time every day (10:00-12:00) gastric infusion, rats in normal control group gives equivalent distilled water, and diabetic model group gives sterilizing camel breast, and gavage volume is 10mLkg -1 .Except Normal group, all the other 5 groups are continued feeding high-sugar-fat-diet, freely drink water, continuous gastric infusion 4 weeks.
4.1.4, ordinary circumstance observe
observe the moisture conditions of rat outward appearance, food-intake, inflow and bedding and padding every day, survey a body weight weekly, tail point gets blood, uses One-Touch blood glucose meter, surveys a fasting blood-glucose (FBG) weekly.
4.1.5, animal processing method
pharmaceutical intervention is after 4 weeks, and each group Rat Fast can't help water 12h, lumbar injection 3% Nembutal sodium solution 30mgkg -1 anesthesia, open belly and get whole blood through abdominal aorta and put common sterile blood sampling pipe, room temperature is centrifugal (3000rmin after leaving standstill 2h -1 , 15min, 4 DEG C), be separated and get serum, packing ,-80 DEG C of preservations; Separately get the heparin tube containing EDTA that blood puts precooling, centrifugal (10000rmin immediately -1 , 10min, 4 DEG C), separated plasma, packing ,-80 DEG C of preservations, collect red blood cell, and the pure water adding precooling is resuspended to original volume, puts-20 DEG C of freezing 60min, takes turns repeatedly cold tactile destruction cell membrane, then by centrifugal for cell suspension (10000rmin through 3 -1 , 10min, 4 DEG C), get supernatant, packing ,-20 DEG C of preservations.Get blood complete, open abdominal cavity immediately and get liver and kidney, after physiological saline rinsing, filter paper blots and weighs, and calculates the liver index (liver weight in wet base/scale of construction × 10 respectively 2 ) and the renal index (kidney weight in wet base/scale of construction × 10 3 ); Get pancreas be placed in 4% paraformaldehyde solution fix, check pathological section to be done.
4.1.6, Testing index and method
full automatic biochemical apparatus is adopted to measure glucose (GLu) in each group of rat blood serum, triglyceride (TG), T-CHOL (TC), low-density lipoprotein (LDL), HDL (HDL), urea nitrogen (BUN), serum creatinine (SCr), glutamic-oxalacetic transaminease (AST), glutamic-pyruvic transaminase (ALT) content.ELISA (ELISA method) measures C peptide (C-peptide) content in rat blood serum.ELISA method measures glycosylated hemoglobin (GHbA1c) content in Rat Erythrocytes lysate.ELISA method measures the content of rat blood serum glucagon-like peptide (GLP-1).
4.2, statistical procedures
data with ± s represents, between each group, first mean carries out homogeneity test of variance, as result meets homogeneity of variance, then adopts one-way analysis of variance or the t method of inspection, if do not met homogeneity of variance, then adopts order Transformation Tests method.All processes is all undertaken by SPSS17.0 statistical analysis software, has the boundary of statistical significance using P < 0.05 as difference.
4.3, experimental result:
4.3.1, mixed culture fermentation degreasing of the present invention camel breast is on the impact of experimental diabetic rats ordinary circumstance and body weight
before pharmaceutical intervention, diabetic model group, positive drug Xi Gelieting group, fermented camel milk high dose group, dosage group in fermented camel milk, fermented camel milk low dose group rat body weight not statistically significant (p>0.05), diabetic model group, positive drug Xi Gelieting group, fermented camel milk high dose group, dosage group in fermented camel milk, fermented camel milk low dose group rat is fed because of high-sugar-fat-diet, diabetic model group before intervening, positive drug Xi Gelieting group, fermented camel milk high dose group, dosage group in fermented camel milk, fermented camel milk low dose group rat body weight is obviously greater than Normal group (p<0.05), rat dead 7 altogether in whole experimentation, wherein, and dead 1 of diabetic model group, dead 1 of positive drug Xi Gelieting group, dead 1 of fermented camel milk high dose group, dead 1 of dosage group in fermented camel milk, dead 3 of fermented camel milk low dose group is each group of diabetes, the possible cause of death: cutization pyogenic infection, DKA, unknown cause seroperitoneum.Finally, totally 63 rats finally complete experiment.As follows respectively:
(1) control group (n=10): in whole experimentation, without rat cadavers, diet, drinking-water are normal, and urine volume is normal, and fur is smooth, more active movability, and the state of mind is good, and body weight increases obviously.
(2) diabetic model group (n=11): in experimentation, diabetes rats diet increases, drinking-water obviously increases, every rat amount of drinking water every day >100ml, few dynamic quiet, One's spirits are drooping, hair color is dark and gloomy, rotten tail point ratio is significantly higher than control group, dead 1 altogether.
(3) positive drug Xi Gelieting group (n=11): in raising, rat general state comparatively diabetic model group obviously improves.Totally 1 rats death.Rat diet, drinking-water are close to normal, and urine volume is on the high side, psychoreaction can, hair color close to normal, without generations such as rotten tail point, skin infections.Pharmaceutical intervention, after 4 weeks, compares with diabetic model group, and body weight significantly increases (p<0.05 the results are shown in Table 6).
(4) fermented camel milk low dose group (n=9): in experiment feeding process, rat general state is good, rat diet, drinks water on the high side, and urine volume is more, and psychoreaction is slightly better than diabetic model group.In feeding process, dead 3 altogether, one group that rats death number is maximum.
(5) dosage group (n=11) in fermented camel milk: in raising, rat general state comparatively diabetic model group group improves.Totally 1 rats death.Rat diet, drink water more, urine volume is on the high side, psychoreaction can, hair color is dark and gloomy, without generations such as rotten tail point, skin infections.Pharmaceutical intervention is after 4 weeks, and middle dosage group compares with diabetic model group, and body weight increases (p<0.05 the results are shown in Table 6).
(6) fermented camel milk high dose group (n=11): in raising, rat general state comparatively diabetic model group obviously improves.Totally 1 rats death.Rat diet, drinking-water are close to normal, and urine volume is on the high side, psychoreaction can, hair color close to normal, without generations such as rotten tail point, skin infections.Pharmaceutical intervention, after 4 weeks, compares with diabetic model group, and body weight significantly increases (p<0.05 the results are shown in Table 6).
4.3.2, the impact of invention mixed culture fermentation degreasing camel breast on diabetes rat fasting blood-glucose
after injection STZ, each group diabetes rat FBG is at 11.1mmolL -1 above, be significantly higher than normal blank group (P<0.05): before pharmaceutical intervention, dosage group, fermented camel milk low dose group rat fasting blood-glucose not statistically significant (p>0.05) in diabetic model group, positive drug Xi Gelieting group, fermented camel milk high dose group, fermented camel milk.Pharmaceutical intervention is after 4 weeks, fermented camel milk high dose group and positive drug group rat fasting blood-glucose are significantly lower than diabetic model group (P<0.05), in fermented camel milk, dosage group fasting blood-glucose is also remarkable in diabetic model group (P<0.05), the results are shown in Table 7.
below in experiment, in experimentation, in dead 1 of rats in normal control group, fermented camel milk, dead 1 of dosage group, is rejected.
4.3.3, the impact of invention mixed culture fermentation degreasing camel breast on experimental diabetic rats blood fat
rat diabetes model group is compared with Normal group, and serum cholesterol value, Triglyceride values and low-density lipoprotein white level raise.The large and small dosage group of fermented camel milk of the present invention and Xi Gelieting group (20 mgkg -1 ) cholesterol (TC) level reduce, LDL-C level reduce, TG level reduce.Dosage group, fermented camel milk high dose group of the present invention and more all can raise HDL-C with Normal group in diabetic model group, Xi Gelieting group, fermented camel milk of the present invention, difference has statistical significance, the results are shown in Table 8.
4.3.4, the impact of mixed culture fermentation degreasing camel breast of the present invention on experimental diabetic rats renal function
diabetic model group rat kidney index, serum BUN, SCr content is all significantly higher than Normal group (P<0.05).Xi Gelieting group, fermented camel milk high, middle dosage group renal index, serum BUN content is all remarkable in diabetic model group (P<0.05), Xi Gelieting group, fermented camel milk high, medium and low dosage group serum SCr content is all remarkable in diabetic model group (P<0.05), the results are shown in Table 9.
4.3.5, the impact of mixed culture fermentation degreasing camel breast of the present invention on experimental diabetic rats liver function
at the end of experiment, diabetic model group rat liver index, serum AST, ALT content are all significantly higher than Normal group (P<0.05).Xi Gelieting group, fermented camel milk high, medium and low dosage group serum AST, ALT content are all remarkable in diabetic model group (P<0.05), the results are shown in Table 10.
4.3.6, the impact of mixed culture fermentation degreasing camel breast of the present invention on experimental diabetic rats CP, IRI, HOMA-IS
at the end of experiment, diabetic model group rat CP value is lower than Normal group (P<0.05), Xi Gelieting group, and fermented camel milk high dose group CP value is significantly higher than diabetic model group (P<0.05).Replace insulin improvement HOMA (CP) formula to evaluate insulin resistance degree with empty stomach CP, formula is IRI=1.5+FBG × empty stomach CP (ng mL -1 × 333)/2800.Diabetic model group IRI value is significantly higher than Normal group (P<0.05), Xi Gelieting group, and fermented camel milk high dose group IRI value is significantly higher than diabetic model group (P<0.05).Replace insulin improvement HOMA (CP) formula to evaluate islet beta cell function with empty stomach CP strong and weak, formula is HOMA-IS (Normal)=0.27 × empty stomach CP (ngmL -1 × 333)/(FBG-3.5)+50; HOMA-IS (DM)=0.27 × empty stomach CP (ngmL -1 × 333)/FBG-3.5.Diabetic model group HOMA-IS value is significantly higher than Normal group (P<0.05), Xi Gelieting group, fermented camel milk high dose group HOMA-IS value is significantly higher than diabetic model group (P<0.05), the results are shown in 11.
4.3.7, the impact of mixed culture fermentation degreasing camel breast of the present invention on experimental diabetic rats Glu, GHbAIc, GLP-1
at the end of experiment, the equal highly significant of diabetic model group rat Glu, GHbAIc content is higher than Normal group, and GLP-1 content is significantly lower than Normal group (P<0.05).Xi Gelieting group Glu, GHbAIc, fermented camel milk middle and high dosage group Glu content, fermented camel milk high dose group GHbAIc content is significantly lower than diabetic model group, Xi Gelieting group, fermented camel milk high, medium and low dosage group GLP-1 content is all significantly higher than diabetic model group (P<0.05), the results are shown in Table 12.
4.3.8, the impact of mixed culture fermentation degreasing camel breast of the present invention on pancreas in diabetic tissue
rat pancreas carries out HE dyeing, and rats in normal control group pancreas leaflet structure is normal, and pancreas islet is circular, similar round, and number is normal, and island inner structure is complete, distinct.Diabetic model group compares with control group, and pancreas islet form is irregular, and number obviously reduces, island inner cell swelling, and part becomes in cavity sample, blood vessel dilatation in island, hyperemia.Xi Gelieting group, the middle and high dosage group of fermented camel milk compares pancreas islet increased number with diabetic model group, and volume increases, and island inner cell number increases, and positive drug group and high dose medicament intervention group are improved then more obvious, the results are shown in Figure 1 to Fig. 6.
the present invention utilizes separation to obtain 10 strains of lactic acid bacteria and 4 Yeasts preparation fermentation degreasing camel breast, high-sugar-fat-diet is utilized to feed 8 weeks, the method establishment diabetes rat model of the low dose of STZ of lumbar injection, observe fermentation degreasing camel breast to each rat model body weight (BW), fasting blood-glucose (FBG), glucose in serum (GLu), glycosylated hemoglobin (GHbAIc) in erythrocyte cracked liquid, the impact of the indexs such as serum C peptide (CP) and four items of blood lipid tests, and replace insulin improvement HOMA (CP) formula to evaluate insulin resistance index (IRI) and islet beta cell function (HOMA-IS) with empty stomach C peptide, measure the content of each group of rat serum creatinine (SCr), urea nitrogen (BUN), glutamic-oxalacetic transaminease (AST), glutamic-pyruvic transaminase (ALT), calculate liver renal index, analyze the impact of fermentation degreasing camel breast on rat model hepatic and renal function, measure the changes of contents of each group of rat blood serum glucagon-like peptide (GLP-1), analyze the mechanism of action of fermentation degreasing camel breast to diabetes rat, pancreatic tissue pathology detect, and carry out HE dyeing, observe pancreatic tissue structure, islet cells form, quantity etc., analyze the impact of fermentation degreasing camel breast on rat model pancreatic tissue.
result shows:
1. pharmaceutical intervention is after 4 weeks, the newborn high dose of fermentation degreasing camel obviously can improve rat model and to lose weight phenomenon, highly significant reduces FBG, GLu and low-density lipoprotein (LDL-C) content (p<0.01), reduce T-CHOL (TC), triglycerides (TG), GHbAIc and IRI(p<0.05), obvious increase HDL (HDL-C) content (p<0.01), increases CP content, HOMA-IS(p<0.05).
2. the equal highly significant of the newborn basic, normal, high dosage of degreasing camel that ferments reduces rat model SCr, ALT, middle and high dosage can highly significant reduce AST(p<0.01), basic, normal, high dosage all significantly reduces liver index, low dosage significantly reduces AST, and middle and high dosage all significantly reduces renal index and BUN(p<0.05).
3. fermentation degreasing camel Ruzhong, high dose all significantly increase serum GLP-1 content (p<0.01), and low dosage significantly can increase serum GLP-1 content (p<0.05).
4. the visible middle and high dosage group pancreas leaflet structure of pancreatic tissue HE dyeing is complete, and islet cells number comparatively diabetic model group increases.Conclusion: fermentation degreasing camel breast can reduce blood glucose in diabetic rats, improve blood sugar disorders and Diabetic liver renal dysfunction, and increasing serum glucagon-like peptide level and improve pancreatic tissue structure, increase islet cells.
the present invention is easy to operation, production method is simple, mouthfeel is good, and gained mixed culture fermentation degreasing camel breast is nutritious, containing compositions such as profitable probliotics, immunomodulatory peptides, anti-oxidation peptides, there is anti-diabetic, anti-inflammatory, the effect such as anti-oxidant, physique and the overall activity of people can be strengthened.
above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can increase and decrease non-essential technical characteristic according to actual needs, meet the demand of different situations.

Claims (9)

1. one kind for antidiabetic mixed culture fermentation degreasing camel breast, it is characterized in that adding Lactobacillus helveticus leavening to degreasing camel Ruzhong, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida ferment-fermented after obtain,
Wherein, the weight ratio between the weight sum of Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening and degreasing camel breast is 1:15 to 25;
Wherein, Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, weight ratio between Pichia membranaefaciens leavening and ethanol Candida leavening is that 0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 to 1.0:0.8 is to 1.0.
2. mixed culture fermentation degreasing according to claim 1 camel breast, is characterized in that obtaining as follows: the first step, degreasing camel breast through pasteurize be cooled to 30 DEG C for subsequent use to about 40 DEG C, second step, the Lactobacillus helveticus leavening of requirement is added to the degreasing camel Ruzhong after pasteurize, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening, at 35 DEG C to 40 DEG C, mixed culture fermentation 8 is little obtained mixed culture fermentation degreasing camel breast up to 12 hours.
3. mixed culture fermentation degreasing camel breast according to claim 2, is characterized in that the condition that the hunchbacked breast of degreasing carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 5min to 10min carries out sterilization, or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous, or/and, by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening add the camel of the degreasing after pasteurize Ruzhong respectively to, or by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening admixed together after add to the degreasing after pasteurize camel Ruzhong.
4. the mixed culture fermentation degreasing camel breast according to claim 1 or 2 or 3, it is characterized in that Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening obtain by following raw material weight ratio, the weight ratio of Lactobacillus helveticus freeze-dried powder or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder and degreasing camel breast is 1:100 to 200,
Wherein, the concrete preparation method of Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is: first degreasing camel breast is carried out pasteurize, then degreasing camel breast is cooled to 30 DEG C to 40 DEG C, then the Lactobacillus helveticus freeze-dried powder of aequum or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder are joined the degreasing camel Ruzhong of aequum, Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or the tough and tensile subspecies leavening of lactobacillus paraceasi class or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is obtained 35 DEG C to 40 DEG C condition bottom fermentations 18 are little up to 24 hours.
5. mixed culture fermentation degreasing camel breast according to claim 4, is characterized in that the condition that the hunchbacked breast of degreasing carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 30min to 60min carries out sterilization; Or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous.
6. a production method for mixed culture fermentation degreasing according to claim 1 camel breast, is characterized in that carrying out in the steps below: the first step, degreasing camel breast through pasteurize be cooled to 30 DEG C for subsequent use to about 40 DEG C, second step, the Lactobacillus helveticus leavening of requirement is added to the degreasing camel Ruzhong after pasteurize, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening, at 35 DEG C to 40 DEG C, mixed culture fermentation 8 is little obtained mixed culture fermentation degreasing camel breast up to 12 hours.
7. the production method of mixed culture fermentation degreasing camel breast according to claim 6, is characterized in that the condition that the hunchbacked breast of degreasing carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 5min to 10min carries out sterilization, or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous, or/and, by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening add the camel of the degreasing after pasteurize Ruzhong respectively to, or by Lactobacillus helveticus leavening, lactobacillus plantarum ferment, lactobacillus paraceasi leavening, the tough and tensile subspecies leavening of lactobacillus paraceasi class, Lactobacillus mucosae leavening, Harbin lactobacillus leavening agent, Lactobacillus hilgardii leavening, Lactobacillus pentosus leavening, Lactobacillus rhamnosus leavening, Lactococcus lactis bacterium leavening agent, Issatchenkia orientalis leavening, kluyveromyces marxianus leavening, Pichia membranaefaciens leavening and ethanol Candida leavening admixed together after add to the degreasing after pasteurize camel Ruzhong.
8. the production method of the mixed culture fermentation degreasing camel breast according to claim 6 or 7, it is characterized in that Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening obtain by following raw material weight ratio, the weight ratio of Lactobacillus helveticus freeze-dried powder or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder and degreasing camel breast is 1:100 to 200,
Wherein, the concrete preparation method of Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or lactobacillus paraceasi class tough and tensile subspecies leavening or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is: first degreasing camel breast is carried out pasteurize, then degreasing camel breast is cooled to 30 DEG C to 40 DEG C, then the Lactobacillus helveticus freeze-dried powder of aequum or Lactobacillus plantarum freeze-dried powder or lactobacillus paraceasi freeze-dried powder or the tough and tensile subspecies freeze-dried powder of lactobacillus paraceasi class or Lactobacillus mucosae freeze-dried powder or Harbin lactobacillus freeze-dried powder or Lactobacillus hilgardii freeze-dried powder or Lactobacillus pentosus freeze-dried powder or Lactobacillus rhamnosus freeze-dried powder or Lactococcus lactis freeze-dried powder or Issatchenkia orientalis freeze-dried powder or kluyveromyces marxianus freeze-dried powder or Pichia membranaefaciens freeze-dried powder or ethanol Candida freeze-dried powder are joined the degreasing camel Ruzhong of aequum, Lactobacillus helveticus leavening or lactobacillus plantarum ferment or lactobacillus paraceasi leavening or the tough and tensile subspecies leavening of lactobacillus paraceasi class or Lactobacillus mucosae leavening or Harbin lactobacillus leavening agent or Lactobacillus hilgardii leavening or Lactobacillus pentosus leavening or Lactobacillus rhamnosus leavening or Lactococcus lactis bacterium leavening agent or Issatchenkia orientalis leavening or kluyveromyces marxianus leavening or Pichia membranaefaciens leavening or ethanol Candida leavening is obtained 35 DEG C to 40 DEG C condition bottom fermentations 18 are little up to 24 hours.
9. the production method of mixed culture fermentation degreasing camel breast according to claim 8, is characterized in that the condition that the hunchbacked breast of degreasing carries out pasteurize is: under temperature is 90 DEG C to 95 DEG C conditions, is incubated 30min to 60min carries out sterilization; Or/and, degreasing camel breast obtains as follows: by the fresh camel breast of requirement, first centrifugal 15min to 20min under rotating speed is 2000r/min to 3000r/min condition, after then discarding upper-layer fat, finally obtains degreasing camel breast under pressure is 1.5Mpa to 1.7Mpa condition after homogeneous.
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