CN104430638A - Plant epidermal tissue repairing agent and application thereof - Google Patents
Plant epidermal tissue repairing agent and application thereof Download PDFInfo
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- CN104430638A CN104430638A CN201410799082.0A CN201410799082A CN104430638A CN 104430638 A CN104430638 A CN 104430638A CN 201410799082 A CN201410799082 A CN 201410799082A CN 104430638 A CN104430638 A CN 104430638A
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- tissue repair
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Abstract
The invention relates to a plant epidermal tissue repairing agent and application thereof, belongs to the field of bio-medicines, and aims to solve the technical problem of providing a plant epidermal tissue repairing agent containing kelp and aloe extracts and a preparation method of the repairing agent. The active ingredient of the plant epidermal tissue repairing agent is prepared by extracting the raw materials kelp and aloe in a weight ratio of (5-15):1, and preferably 10:1. The plant epidermal tissue repairing agent provided by the invention has excellent repairing effect and low preparation cost, does not contain other harmful substances, and has the characteristic of environmental friendliness and safety.
Description
Technical field
The present invention relates to the agent of plant epidermis tissue repair and application thereof, belong to biomedicine field.
Background technology
Kelp is the herbal medicine that " Chinese Pharmacopoeia " includes, and medicinal source is the dry thallus of Laminariaceae plant sea-tangle or Alariaceae plant kelp (goose palm dish).Kelp frond olive-drab is crineous after dry.After ripe, keratin is in banded, general long 2-6m, wide 20-50cm, two parallel shallow ridges indulged away are had at central vane, in the middle of two ditches, thicker part is " middle band portion ", and thickness is about 2-5mm, and both sides of the edge are gradually thin, and have wavy gauffer, blade base wedge shape, the thick one-tenth stage is then oblate, under have a cylindrical or oblate short handle, long 5-15cm, handle and blade interior form by marrow, cortex and epidermal area.In exoderm, have mucilage cavity, in chamber, have secretory cell, can secreting mucus to leaf surface, form gelatinous layer, make frond stick-slip and shield.Marrow is made up of many algal filaments, and algal filament cell one end is expanded in loudspeaker tubulose.Frond juvenile stage blade face is smooth, and concavo-convex phenomenon appears in little sea-tangle phase blade.Annual frond blade bottom, can see sporangiorus growth, usually in subcircular patch shape; Biennial frond almost all grows sporangiorus on whole blade.Holdfast is formed by dichotomous rhizoid.Autumn in spore maturing stage.Kelp gas raw meat, taste is salty.Return liver, stomach, kidney channel.There is softening and resolving hard mass, dissolving phlegm, the function of sharp water.Kelp is the marine alga that a kind of amount of iodine is very high, also be the vegetables that a kind of nutritive value is very high, its chemical composition is containing phycocolloid (algin), mannitol (mannitol), galactan (galactan), laminine (laminine), sea-tangle glycan (laminarin), glutamic acid, aspartic acid, proline, vitamin B1, vitamin C, citrin and iodine, potassium etc.
Aloe (formal name used at school: Aloe vera) Aloe is Liliaceae perennial evergreen herbaceous plant, and leafage is raw, large and plump, in seat shape or be born in stem top, the normal lanceolar of leaf or leaf short wide, edge has sharp tooth to sting.Inflorescence is umbrella shape, total shape, spike, taper shape etc., and look is red, yellow or tool redness of the skin or complexion spot, six, petal, gynoecium six pieces.The many Colaesces of perianth base portion become tubular.Aloe integrates edible, medicinal, improves looks, views and admires plant nova.It is secreted effluent (principle active component is the green onion quinones substances such as aloin) and has been widely applied in medicine and daily use chemicals.Aloe folks of china just by as beauty treatment, hair care and treatment disease of skin natural drug.Aloe Vera Gel stings certain itching-relieving action to mosquito.Also there is sterilization antiinflammatory action, under stomach invigorating, let out effect, the cardiac stimulant effect of invigorating blood circulation, immunity and palingenesis, immunity and antitumor action, detoxication, anti-aging effects, analgesia, sedation, anti-sunlight function etc.The main extract aloin of aloe, its chemical name 10-glucopyranosyl-1,8-dihydroxy-3-(methylol)-9 (10H)-anthrone.The mixture of active component based on barbaloin is dicotyledon aloe extract.Aloin A, wound hormone and glycan peptide sweet dew (Ke-2) etc. have viral infection resisting, promote the effect that wound healing is restored, have the function of anti-inflammation and sterilization, heat absorption detumescence, softening skin, maintenance cell viability, curdlan is combined with traumatic acid and is also had healing wounds activity.
Summary of the invention
Technical problem to be solved by this invention is to provide the agent of a kind of plant epidermis tissue repair and application thereof.
Plant epidermis tissue repair of the present invention agent, its active component is extracted by the raw material of following weight ratio and is prepared from: kelp: aloe=5 ~ 15:1, preferred kelp: aloe=10:1.
Plant epidermis tissue repair agent described above, its active component is obtained through alcohol extracting by kelp and aloe, and in order to make the agent of plant epidermis tissue repair better be extracted, our preferred alcohol is as the extractant of effective component extracting.
Plant epidermis tissue repair agent described above, kelp and aloe comprise the following steps through the method for alcohol extract:
A, kelp, through removal of impurities, rinse process, and aloe is shone after dry treatment through removal of impurities, and mixing, shatters, and cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve, obtains mixed powder;
B, mixed powder are dissolved in absolute ethyl alcohol, obtain filtrate after stirring, heating, refluxing extraction process;
C, filtrate simmer down to 1/40 ~ 1/60 volume, be preferably 1/50 volume, obtains phycocolloid element and aloin mixture.
In the preparation method of plant epidermis tissue repair agent described above, the volume of absolute ethyl alcohol in described b step: the volume=5 ~ 15:1 of mixed powder, is preferably 10:1; Stirring frequency is 60 ~ 120 rpms, is preferably 80 ~ 100 rpms; Heating-up temperature is 60 ~ 95 DEG C, is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Heat time is 2 ~ 4 hours, is preferably 3.5 hours; Refluxing extraction number of times is 1 ~ 3 time, is preferably 2 times.
Above-mentioned plant epidermis tissue repair agent, its active component is made up of the raw material of following weight proportioning: phycocolloid element: aloin=5 ~ 15:1, preferred phycocolloid element: aloin=10:1.
Wherein, plant epidermis tissue repair described above agent is phycocolloid element and aloin mixture aqua.
Aqua described above is made up of the component of following weight portion: phycocolloid element and 25 ~ 35 parts, aloin mixture, absolute ethyl alcohol 5 ~ 15 parts, 2 ~ 10 parts, surfactant, 50 ~ 60 parts, water composition.
Further, in order to obtain better scheme, preferred phycocolloid element and 29 parts, aloin mixture, absolute ethyl alcohol 10 parts, 6 parts, surfactant, 55 parts, water composition.
Wherein said surfactant is alkyl glucoside surfactant, and it has the features such as high surface, good ecological security and intermiscibility, is internationally recognized first-selection " green " function surface activating agent.
Plant epidermis tissue repair agent described above can be applied to fruit and the damaged plant epidermis tissue repair aspect of blade.
The present inventor, through great many of experiments, confirms the extract phycocolloid element of kelp and the extract aloin of aloe, can play the effect of reparation after treatment to plant epidermis tissue.Have broad application prospects at biomedical sector.
Plant epidermis tissue repair agent prepared by the present invention has good repairing effect, and preparation cost is low, and preparation process is simple and convenient, and composition containing other harmful substance, does not have the feature of environmental protection.
Embodiment
Plant epidermis tissue repair of the present invention agent, its active component is extracted by the raw material of following weight ratio and is prepared from: kelp: aloe=5 ~ 15:1, preferred kelp: aloe=10:1.
Plant epidermis tissue repair agent described above, its active component is obtained through alcohol extracting by kelp and aloe, and in order to make the agent of plant epidermis tissue repair better be extracted, our preferred alcohol is as the extractant of effective component extracting.
Plant epidermis tissue repair agent described above, kelp and aloe comprise the following steps through the method for alcohol extract:
A, kelp, through removal of impurities, rinse process, and aloe is shone after dry treatment through removal of impurities, and mixing, shatters, and cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve, obtains mixed powder;
B, mixed powder are dissolved in absolute ethyl alcohol, obtain filtrate after stirring, heating, refluxing extraction process;
C, filtrate simmer down to 1/40 ~ 1/60 volume, be preferably 1/50 volume, obtains phycocolloid element and aloin mixture.
In the preparation method of plant epidermis tissue repair agent described above, the volume of absolute ethyl alcohol in described b step: the volume=5 ~ 15:1 of mixed powder, is preferably 10:1; Stirring frequency is 60 ~ 120 rpms, is preferably 80 ~ 100 rpms; Heating-up temperature is 60 ~ 95 DEG C, is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Heat time is 2 ~ 4 hours, is preferably 3.5 hours; Refluxing extraction number of times is 1 ~ 3 time, is preferably 2 times.
Above-mentioned plant epidermis tissue repair agent, its active component is made up of the raw material of following weight proportioning: phycocolloid element: aloin=5 ~ 15:1, preferred phycocolloid element: aloin=10:1.
Wherein, plant epidermis tissue repair described above agent is phycocolloid element and aloin mixture aqua.
Aqua described above is made up of the component of following weight portion: phycocolloid element and 25 ~ 35 parts, aloin mixture, absolute ethyl alcohol 5 ~ 15 parts, 2 ~ 10 parts, surfactant, 50 ~ 60 parts, water composition.
Further, in order to obtain better scheme, preferred phycocolloid element and 29 parts, aloin mixture, absolute ethyl alcohol 10 parts, 6 parts, surfactant, 55 parts, water composition.
Wherein said surfactant is alkyl glucoside surfactant, and it has the features such as high surface, good ecological security and intermiscibility, is internationally recognized first-selection " green " function surface activating agent.
Plant epidermis tissue repair agent described above can be applied to fruit and the damaged plant epidermis tissue repair aspect of blade.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 the present invention contains the preparation of the composition tissue repair agent of phycocolloid element and aloin extract
A, extraction: take kelp, removing impurity, cleans, slightly moistens, cut sheet, dry, take aloe, removing impurity, clean, run through, cut sheet or section, dry, by kelp and aloe mixing, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filters, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract bq-01 ~ bq-06; Wherein, each parameter is in table 1;
B, preparation: in extract bq-01 ~ bq-06, add auxiliary agent in table 2, stir, must contain the composition pesticide BQ-01 ~ BQ-06 of kelp skin and aloe extract.
Table 1
Wherein, the kelp used in the present invention and aloe are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2
Embodiment 2 the present invention contains the preparation of the composition tissue repair agent of phycocolloid element and aloin extract
A, extraction: take kelp, removing impurity, cleans, slightly moistens, cut sheet, dry, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain kelp extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take aloe, removing impurity, cleans, runs through, cut sheet or section, dry, pulverize, cross 80 mesh sieves, add solvent, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract solution from aloe d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take kelp extract, extract solution from aloe and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide BQ-07 ~ BQ-12 containing kelp and aloe extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Embodiment 3 the present invention contains the preparation of the composition tissue repair agent of phycocolloid element and aloin extract
Take phycocolloid element and aloin and auxiliary agent according to proportioning in table 6, mixing, stirring, stirring and evenly mixing, obtains the composition pesticide BQ-13 ~ BQ-18 containing the root bark of shaggy-fruited dittany and madder extract.
Table 6
Wherein, phycocolloid element and aloin are all purchased from Xi'an Lin He Bioisystech Co., Ltd.
Experimental example 1 tissue repair oranges and tangerines are tested
According to action principle and the mode of the agent of plant epidermis tissue repair, the fruit of oranges and tangerines normal growth and leaf is selected to carry out this time experiment.
1 materials and methods
1.1 reagent agent
Tissue repair agent
1.2 Treatment Design
A. tissue repair agent 200 doubly sprays
B. clear water contrast
1.3 test method
Test arrangement on the large pool of Pujiang, the field gross area 2 mu, random selecting 6 communities, the strain of every community 4 set, utilize sand paper artificial on citrusfruit, blade, cause about 1cm
2minor wound, every strain 30 fruits, 30 leaves, with redness little label mark, carry out the process of tissue repair agent spray medicine, dispenser 3 times, every minor tick 3 days to wherein random 2 two trees, do clear water contrast for another 2.3rd time spray medicine observed drug effect after 15 days.
1.4 investigation method
Observe each fruit, the wound healing of every sheet leaf, reply situation, the contrast of contrast agents group, clear water and intact fruit, interlobate difference.
1.5 safety observations
Medicament is irregularly observed on the impact of fruit, leaf growth after dispenser.
2 results and analysis
According to above-mentioned stage division and data processing formula statistics dispenser before and after dispenser 15 days total leaf number, fruit number and be repaired the number of blade and fruit number, calculate control efficiency, the results are shown in Table 7.
Table 7
Note: D1, D2 are natural extraction phycocolloid element and aloin.
The composition pesticide (BQ-07 ~ BQ-12) be mixed to get after independent extraction is imitated in the treatment of the composition pesticide (BQ-01 ~ BQ-06) adopting common extracting method to make, visible common extraction contributes to improving dissolution rate, improves germicidal efficiency; And the result for the treatment of of aloin single dose D2 that the phycocolloid element single dose D1 be prepared from than single Thallus Laminariae (Thallus Eckloniae) extract and aloe extract are prepared from is good, visible, has synergistic function between product compositions of the present invention.
Meanwhile, adopt the preventive effect of phycocolloid element and the composite composition pesticide (BQ-13 ~ BQ-18) obtained of aloin also apparently higher than phycocolloid element single dose D1 and aloin single dose D2, visible, phycocolloid is plain and aloin is composite has synergistic function.
In addition, have no during dispenser and have poisoning to show to plant.
Claims (10)
1. plant epidermis tissue repair agent, is characterized in that: its active component is extracted by the raw material of following weight ratio and is prepared from: kelp: aloe=5 ~ 15:1, preferred kelp: aloe=10:1.
2. plant epidermis tissue repair according to claim 1 agent, is characterized in that: its active component is obtained through alcohol extracting by kelp and aloe.
3. plant epidermis tissue repair according to claim 2 agent, is characterized in that: its active component is obtained through alcohol extract by kelp and aloe.
4. plant epidermis tissue repair according to claim 3 agent, is characterized in that: kelp and aloe comprise the following steps through the method for alcohol extract:
A, kelp, through removal of impurities, rinse process, and aloe is shone after dry treatment through removal of impurities, and mixing, shatters, and cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve, obtains mixed powder;
B, mixed powder are dissolved in absolute ethyl alcohol, obtain filtrate after stirring, heating, refluxing extraction process;
C, filtrate simmer down to 1/40 ~ 1/60 volume, be preferably 1/50 volume, obtains phycocolloid element and aloin mixture.
5. plant epidermis tissue repair according to claim 4 agent, is characterized in that: the volume of absolute ethyl alcohol in described b step: the volume=5 ~ 15:1 of mixed powder, is preferably 10:1; Stirring frequency is 60 ~ 120 rpms, is preferably 80 ~ 100 rpms; Heating-up temperature is 60 ~ 95 DEG C, is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Heat time is 2 ~ 4 hours, is preferably 3.5 hours; Refluxing extraction number of times is 1 ~ 3 time, is preferably 2 times.
6. the plant epidermis tissue repair agent according to any one of Claims 1 to 5, is characterized in that: its active component is made up of the raw material of following weight proportioning: phycocolloid element: aloin=5 ~ 15:1, preferred phycocolloid element: aloin=10:1.
7. plant epidermis tissue repair according to claim 6 agent, is characterized in that: the formulation of described plant epidermis tissue repair agent is aqua.
8. plant epidermis tissue repair according to claim 7 agent, it is characterized in that: described aqua is made up of the component of following weight portion: phycocolloid element and 25 ~ 35 parts, aloin mixture, absolute ethyl alcohol 5 ~ 15 parts, 2 ~ 10 parts, surfactant, 50 ~ 60 parts, water composition; Preferred phycocolloid element and 29 parts, aloin mixture, absolute ethyl alcohol 10 parts, 6 parts, surfactant, 55 parts, water composition.
9. plant epidermis tissue repair according to claim 8 agent, is characterized in that: described surfactant is alkyl glycoside.
10. the application of the plant epidermis tissue repair agent described in any one of claim 1 ~ 9 in fruit and the damaged plant epidermis tissue repair of blade.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410799082.0A CN104430638A (en) | 2014-12-19 | 2014-12-19 | Plant epidermal tissue repairing agent and application thereof |
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| CN201410799082.0A CN104430638A (en) | 2014-12-19 | 2014-12-19 | Plant epidermal tissue repairing agent and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116210730A (en) * | 2021-12-02 | 2023-06-06 | 四川省新兰月生物科技有限公司 | Pesticide composition for preventing sunscald of citrus |
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| CN102124911A (en) * | 2010-11-29 | 2011-07-20 | 天津滨海国际花卉科技园区股份有限公司 | Method for promoting wound healing after soft pinching of jonquils |
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2014
- 2014-12-19 CN CN201410799082.0A patent/CN104430638A/en active Pending
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| CN1269339A (en) * | 1999-04-01 | 2000-10-11 | 美国金秋公司 | Method for producing extracted liquor of seaweed for promoting growth of plant, obtained seaweed extracted liquor and compound fertilizer thereof |
| CN1483451A (en) * | 2003-07-18 | 2004-03-24 | 王舒妍 | Method for extracting 50% aloetin |
| EP2238981A1 (en) * | 2007-09-21 | 2010-10-13 | Pharmabrand S.A. | Composition comprising mucilaginous polysaccharides derived from aloe barbadensis combined with liposomes, method for obtaining same and use thereof as a sun protector, an anti-acne agent, an epidermal regenerator and a moisturiser |
| CN101700042A (en) * | 2009-10-19 | 2010-05-05 | 集美大学 | Method for producing antibacterial plant growth regulator for promoting growth of plants |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116210730A (en) * | 2021-12-02 | 2023-06-06 | 四川省新兰月生物科技有限公司 | Pesticide composition for preventing sunscald of citrus |
| CN116210730B (en) * | 2021-12-02 | 2024-04-02 | 四川省新兰月生物科技有限公司 | Pesticide composition for preventing sunscald of citrus |
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