CN104429946A - Composite culture medium liquid for tissue culture of dendrobium officinale reproduced by utilizing stem tip as explant - Google Patents
Composite culture medium liquid for tissue culture of dendrobium officinale reproduced by utilizing stem tip as explant Download PDFInfo
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- CN104429946A CN104429946A CN201410634111.8A CN201410634111A CN104429946A CN 104429946 A CN104429946 A CN 104429946A CN 201410634111 A CN201410634111 A CN 201410634111A CN 104429946 A CN104429946 A CN 104429946A
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Abstract
The invention discloses composite culture medium liquid for tissue culture of dendrobium officinale reproduced by utilizing a stem tip as explant. The composite culture medium liquid comprises a basic culture medium which is a combination of an LS culture medium and a WPM culture medium according to a concentration ratio of (3-3.5):1; and the composite culture medium liquid also comprise the following substances of various types and concentration: 5-10g/L of peptone, 1-3g/L of agar, 0.5-2g/L of cane sugar and 0.01-0.05mg/L of plant hormones IBA. The culture medium liquid provided by the invention is suitable for tissue culture by utilizing the stem tip as explant, globoid or adventitious buds can grow, bottle seedlings can grow in geometric progression by virtue of slitting, bottle replacement and other means, and the survival rate is effectively improved.
Description
Technical field
The present invention relates to a kind of liquid medium of tissue culture of dendrobium candidum.
Background technology
Dendrobium candidum (formal name used at school: Dendrobium officinale Kimura et Migo), has another name called: ribbed hedyotis herb, Yunnan iron sheet.Belong to micro-specific item, the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family.Stem is upright, and cylindrical, long 9-35 centimetre, thick 2-4 millimeter, sepal and petal yellow green, closely similar, oval shape lanceolar, is about 1.8cm, wide 4 ~ 5mm, 3 ~ June of florescence.Mainly be distributed in the ground such as Chinese Anhui, Zhejiang, Fujian.Its stem is used as medicine, and belongs to the yin tonics in tonic: reinforcing stomach reg fluid, nourishing Yin and clearing heat.
Current existing candidum tissue culturing cultural method mainly with stem apex, blade tip for explant, first form unrooted seedling in the medium, then carry out culture of rootage.
Summary of the invention
The technical problem to be solved in the present invention overcomes existing defect, provides a kind of compound criteria base fluid utilizing stem apex to carry out the tissue culture of the dendrobium candidum of breeding as explant.
Object of the present invention carrys out specific implementation by the following technical programs:
Utilize stem apex to carry out a compound criteria base fluid for the tissue culture of the dendrobium candidum of breeding as explant, comprise minimal medium be LS medium, WPM medium by (3-3.5): the mixing of the concentration of 1, and be added with the material of following kind and concentration:
Peptone 5-10g/L, agar 1-3g/L, sucrose 0.5-2g/L, plant hormone IBA 0.01-0.05mg/L.
Preferably, the above-mentioned stem apex that utilizes carries out the compound criteria base fluid of the tissue culture of the dendrobium candidum of breeding as explant, comprise that minimal medium is LS medium, WPM medium by the mixing of the concentration of 3:1, and is added with the material of following kind and concentration:
Peptone 9g/L, agar 2.5g/L, sucrose 1.2g/L, plant hormone IBA 0.03mg/L.
Beneficial effect of the present invention:
Liquid medium provided by the invention, be applicable to utilize stem apex to grow as the group cultivation of explant, can grow globoid or indefinite bud, through cutting and changing the formalities such as bottle, bottle seedling number can become geometric progression to grow up, and effectively raise survival rate.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the present invention, is not intended to limit the present invention.
embodiment 1:
Utilize stem apex to carry out a compound criteria base fluid for the tissue culture of the dendrobium candidum of breeding as explant, comprise that minimal medium is LS medium, WPM medium by the mixing of the concentration of 3:1, and is added with the material of following kind and concentration:
Peptone 9g/L, agar 2.5g/L, sucrose 1.2g/L, plant hormone IBA 0.03mg/L.
embodiment 2:
Utilize stem apex to carry out a compound criteria base fluid for the tissue culture of the dendrobium candidum of breeding as explant, comprise that minimal medium is LS medium, WPM medium by the mixing of the concentration of 3:1, and is added with the material of following kind and concentration:
Peptone 10g/L, agar 1g/L, sucrose 2g/L, plant hormone IBA 0.01mg/L.
embodiment 3:
Utilize stem apex to carry out a compound criteria base fluid for the tissue culture of the dendrobium candidum of breeding as explant, comprise that minimal medium is LS medium, WPM medium by the mixing of the concentration of 3.5:1, and is added with the material of following kind and concentration:
Peptone 5g/L, agar 3g/L, sucrose 0.5g/L, plant hormone IBA 0.05mg/L.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. the compound criteria base fluid of the tissue culture of the dendrobium candidum utilizing stem apex to carry out breeding as explant, it is characterized in that: comprise minimal medium be LS medium, WPM medium by (3-3.5): the mixing of the concentration of 1, and be added with the material of following kind and concentration:
Peptone 5-10g/L, agar 1-3g/L, sucrose 0.5-2g/L, plant hormone IBA 0.01-0.05mg/L.
2. the compound criteria base fluid utilizing stem apex to carry out the tissue culture of the dendrobium candidum of breeding as explant according to claim 1, it is characterized in that: comprise that minimal medium is LS medium, WPM medium by the mixing of the concentration of 3:1, and is added with the material of following kind and concentration:
Peptone 9g/L, agar 2.5g/L, sucrose 1.2g/L, plant hormone IBA 0.03mg/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410634111.8A CN104429946A (en) | 2014-11-12 | 2014-11-12 | Composite culture medium liquid for tissue culture of dendrobium officinale reproduced by utilizing stem tip as explant |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410634111.8A CN104429946A (en) | 2014-11-12 | 2014-11-12 | Composite culture medium liquid for tissue culture of dendrobium officinale reproduced by utilizing stem tip as explant |
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| CN104429946A true CN104429946A (en) | 2015-03-25 |
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| CN201410634111.8A Pending CN104429946A (en) | 2014-11-12 | 2014-11-12 | Composite culture medium liquid for tissue culture of dendrobium officinale reproduced by utilizing stem tip as explant |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182791A (en) * | 2017-07-18 | 2017-09-22 | 合肥百绿盛农业科技有限公司 | A kind of bletilla striata tissue culture and rapid propagation method |
Citations (6)
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| CN1401223A (en) * | 2002-09-10 | 2003-03-12 | 上海中医药大学 | Method for production of medicinal material using culture of Dendrobium officinale hormone autotrophic stem tip |
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN101258835A (en) * | 2008-04-23 | 2008-09-10 | 昆明理工大学 | Fast reproducing method for high quality seedling of dendrobium officinale |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
| CN103155871A (en) * | 2013-03-07 | 2013-06-19 | 华中科技大学 | Dendrobium officinale sprout rapid propagation method with high efficiency |
| CN103314859A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Stem tip tissue culture propagation method of dendrobium officinale |
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2014
- 2014-11-12 CN CN201410634111.8A patent/CN104429946A/en active Pending
Patent Citations (6)
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|---|---|---|---|---|
| CN1401223A (en) * | 2002-09-10 | 2003-03-12 | 上海中医药大学 | Method for production of medicinal material using culture of Dendrobium officinale hormone autotrophic stem tip |
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN101258835A (en) * | 2008-04-23 | 2008-09-10 | 昆明理工大学 | Fast reproducing method for high quality seedling of dendrobium officinale |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
| CN103155871A (en) * | 2013-03-07 | 2013-06-19 | 华中科技大学 | Dendrobium officinale sprout rapid propagation method with high efficiency |
| CN103314859A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Stem tip tissue culture propagation method of dendrobium officinale |
Non-Patent Citations (3)
| Title |
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| SAIMA ANJUM,ET AL.: "Investigations of different strategies for high frequency regeneration of Dendrobium malones ‘Victory’", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》, vol. 5, no. 19, 31 October 2006 (2006-10-31), pages 1738 - 1743 * |
| 曾万勇等: "铁皮石斛无菌萌发及小苗快繁培养条件研究", 《武汉工业学院学报》, vol. 31, no. 3, 30 September 2012 (2012-09-30), pages 10 - 14 * |
| 王桥美等: "铁皮石斛愈伤组织诱导条件研究进展", 《农业实用技术》, 31 December 2012 (2012-12-31), pages 19 - 20 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182791A (en) * | 2017-07-18 | 2017-09-22 | 合肥百绿盛农业科技有限公司 | A kind of bletilla striata tissue culture and rapid propagation method |
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Application publication date: 20150325 |
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