CN104411370B - The decomposition accelerating agent of volatility organohalogen compound - Google Patents
The decomposition accelerating agent of volatility organohalogen compound Download PDFInfo
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- CN104411370B CN104411370B CN201380032349.7A CN201380032349A CN104411370B CN 104411370 B CN104411370 B CN 104411370B CN 201380032349 A CN201380032349 A CN 201380032349A CN 104411370 B CN104411370 B CN 104411370B
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- accelerating agent
- decomposition
- volatility
- decomposition accelerating
- organohalogen compound
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
Decomposition accelerating agent is provided, it is to utilize the decomposition accelerating agent used in the purification (biodegradation) of microorganism by volatility organohalogen compound contaminated land, by improving the degrading activity of microorganism, being particularly the microbial activity of administration starting stage, volatility organohalogen compound can be made promptly innoxious.A kind of decomposition accelerating agent of volatility organohalogen compound, it is characterised in that it contains a kind or two or more in following (A)~(C).(A) pericarp of extract (B) citrus that the fruit of citrus is either obtained by the fruit or extract (C) blend (1) glycerine (2) lactoprotein and/or yeast extract (3) vitamin B12 containing following (1)~(3) whole obtained by the pericarp.
Description
Technical field
The present invention relates to the decomposition accelerating agent of volatility organohalogen compound and promotion method is decomposed, specifically,
Relate to promote the decomposition accelerating agent of volatility organohalogen compound decomposition using microorganism and promoted using its decomposition
Enter method.
Background technology
The soil in soil, underground water are sometimes by natural or artificial various chemical contaminations.Such soil is dirty
Dye is either using the soil as the arable land for grain-production in use, being also attempt to all become in inhabitation, commercial exploitation
Into significant problem.
But diet product with immediate access, the difference such as clothes/cosmetics/ornament that contacts skin, the dirt in soil
Dye, particularly soil pollution less attract attention, and also there is the example that landfill chemical substance is handled of digging a hole in the past.
The purification of the pollution in such soil recently becomes significant problem, is directed to this, proposes and has carried out various purifications
Processing method.
If the purification method in the rough classification soil, following four can be categorized as：By being heated at high temperature chemical decomposition
Material makes it be adsorbed in the physical treatment of activated carbon etc.；The innoxious chemical treatment of chemical substance is made by chemical reaction；Profit
With the microbial cleaning method of the microorganism with chemical decomposition physical capacity；And using with absorbing or adsorption of chemical species
The plant purification method of the plant of ability.
Wherein, got most of the attention recently in terms of it can suppress the worry to environment, cost using the method for microorganism, plant,
" biodegradation " is also referred to as using the purification method of microorganism, is also referred to as " plant degradation " using the purification method of plant,
Various research and development are carried out, from the bulky aspect of treatable soil, actively carry out biodegradable grind
Study carefully exploitation.
Biodegradation has two kinds of technologies.A kind of is to have predefined point for object pollution material in polluted place application
Solution plays the technology of the microorganism of effect, is referred to as biological reinforced.Another kind be by the indigenous microorganism to polluted place to
Oxygen, nutrient source are given, makes the activity activation of microorganism, promotes the technology of catharsis, be referred to as biostimulation.
In addition, biodegradation has two methods.One kind is to remove contaminated soil, underground water, is carried out in other places
The method of processing, it is referred to as " processing of facility type ".Another kind is the method for purifying contaminated soil, underground water in the place,
It is referred to as " original position purification ".
As the polluter for turning into significant problem in recent years, have with tetrachloro-ethylene, trichloro ethylene, dichloroethylene, bioxin
The organochlorine compounds such as class, polychlorinated biphenyl are the organohalogen compound of representative.Wherein, on tetrachloro-ethylene, trichloro ethylene,
The volatility organohalogen compound such as dichloroethylene, worry by influence of the breathing to human body, therefore common in need locate as early as possible
The situation of reason.
The volatility organohalogen compound is easily impregnated with to soil, reach Di Xia Shui Veins, easily expand pollution to larger
Scope.Although there is also in general soil more connects the edaphon of volatility organohalogen compound as decomposition
Near surface organic matter is more, and edaphon is also largely present, and with from top layer to deep organic matter and edaphon all
Reduce, the activity of microorganism is reduced to less than the 1/100 of top layer if more than 1m is deep into.Accordingly, once being waved
Hair property organohalogen compound contaminated land has long-time low concentration and contaminated to a wide range.
So low concentration and to a wide range the purification in contaminated soil with biodegradation for effective means, so
Propose the various schemes using the pollution of purifying volatile organohalogen compound as purpose.
Such as propose following scheme：Using more comprising PLA and glycerine, xylitol, D-sorbite, pentaerythrite etc.
The biostimulation of the composition of the ester of functional alcohol (for example, see patent document 1)；Using including yeast, aliphatic acid, carbon hydrate
The biostimulation of the composition of thing etc. (for example, see patent document 2)；Utilize amino acid and the condensation reaction products of hydroxycarboxylic acid
Biostimulation (for example, see patent document 3).It should be noted that having been recorded in non-patent literature 1 promotees various decompose
Enter the method that agent contacts with soil and/or underground water existing for microorganism.
On the other hand, have as known to the microorganism used in the purification of volatility organohalogen compound contaminated land
Anaerobic Bacteria, particularly dehalogenation intend Coccus bacterium.The state being not present if the microorganism, then volatility organic halogen
Untill compound will not decompose to final ethene, but decompose the dichloroethylene that may be parked in as intermediate material, it is thus possible to
Can cause completely to be purified.But, it is also known that have：Even such as dehalogenation intends Coccus bacterium also generally according to it
Species determines decomposable compound, needed from tetrachloro-ethylene into the decomposition of ethene a variety of dehalogenations intend coccuses participate in (referring to
Non-patent literature 2).Therefore, the various volatility organohalogen compound decomposition rates of dehalogenation plan Coccus bacterium might not
It hurry up, in addition, intending the also not necessarily decomposition of participation volatility organohalogen compound of Coccus bacterium even if dehalogenation be present.
It is therefore proposed that there is following scheme：Using multiple dehalogenations intend Coccus bacterium hybrid bacterial strain it is biological reinforced (such as
Referring to non-patent literature 3)；Intend life of the Coccus bacterium with streptococcus as the flora (consortia) of main thalline using dehalogenation
Thing is strengthened (for example, see patent document 4).However, using the hybrid bacterial strain method there is also by the pollution condition of soil, pH,
And then the content of organic matter and the problem of cause purification speed to postpone.
Prior art literature
Patent document
Patent document 1：Japanese Unexamined Patent Application Publication 2000-511969 publications
Patent document 2：Japanese Unexamined Patent Publication 2005-185870 publications
Patent document 3：Japanese Unexamined Patent Publication 2010-104962 publications
Patent document 4：Japanese Unexamined Patent Publication 2011-244769 publications
Non-patent literature
Non-patent literature 1：Co., Ltd.'s census of manufacturing meeting " chemical devices " in July, 2007 number, the rugged abundant " soil/underground in mountain
(Co., Ltd.'s work industry Tone Check meetings " chemical devices " in July, 2007 number, mountain is rugged for water treatment technology-VOC decomposing, purifying technology-"
Abundant " native Reel ground water cleanings Ji Intraoperative-VOC decomposing, purifying Ji Intraoperative-")
Non-patent literature 2：Rugged original contains him, and " vinyl chloride class intends coccus as the dehalogenation in the original position biodegradation of object
The behavior for belonging to bacterium parses " on underground water/soil pollution and its research of Prevent countermeasures rally speech collection (2008) (rugged former Sheng
His " Network ロ ロ エ テ Application Class The object と original position バ イ オ エ メ デ ィ エ ー シ ョ Application To お け Ru
Dehalococcoides category Fine bacterium Behavior Move parsings " underground water Tu Reel pollution dye と そ Prevent countermeasures に Seki The Ru research collection
Hui Talk drill collection (2008))
Non-patent literature 3：Arrow wood, which is cultivated one's moral character " present situation of land remediation technology with prospect " from now on, " seeks food and environment
(arrow wood is cultivated one's moral character " repaiies Complex Ji Intraoperative in soil to harmful chemical-main idea collection page 10 in safety-agricultural aquatic ecosystem system " (2007)
Now shape と the presents Hou is looked forward to " " the safe The in Shi と Ring borders seeks め て-Farming woods aquatic ecosystems system To お け Ru harmful chemicals things
The ペ ー ジ " (2007) of Quality-main idea collection 10)
The content of the invention
Problems to be solved by the invention
However, the method described on patent document 1, patent document 3, the activity of the microorganism of administration starting stage
It is low, therefore, for the contaminated soil particularly largely containing volatility organohalogen compound, particularly underground water, exist net
Change the problem of processing speed is low, innoxious required time is long.In addition, the method described on patent document 2, although there is rush
Enter the effect of the decomposition reaction of volatility organohalogen compound, but the activation of microorganism is still insufficient, still exists innoxious
The problem of required time is long.On the other hand, the method that hybrid bacterial strain is used as disclosed in non-patent literature 3, patent document 4,
There is also because of the pollution condition of soil, pH and then the content of organic matter and caused by purification speed postpone the problem of.
Therefore, it is an object of the invention to provide decomposition accelerating agent, it is polluted by volatility organohalogen compound
The decomposition accelerating agent used in the purification (biodegradation) of land use microorganism, especially by the raising administration starting stage
The activity of microorganism, volatility organohalogen compound can be made innoxious rapidly.
It is further an object that provide the side for promoting volatility organohalogen compound to decompose using microorganism
Method, it is when being purified (biodegradation) using microorganism by volatility organohalogen compound contaminated land, particularly
By the activity for the microorganism for improving the administration starting stage, the rapid innoxious side of volatility organohalogen compound can be made
Method.
The solution used to solve the problem
The present inventor etc. have carried out various researchs in order to reach above-mentioned purpose, as a result find, use following (A)~(C)
In a kind or during two or more decomposition accelerating agent as volatility organohalogen compound, can be effectively facilitated volatility has
Machine halogen compounds utilizes the decomposition of microorganism, so as to complete the present invention.
That is, the decomposition accelerating agent of volatility organohalogen compound of the invention is characterised by, its contain following (A)~
(C) a kind in or two or more.
(A) fruit of citrus or the extract obtained by the fruit
(B) pericarp of citrus or the extract obtained by the pericarp
(C) containing the whole blend in following (1)~(3)
(1) glycerine
(2) lactoprotein and/or yeast extract
(3) vitamin B12
Moreover it is preferred that when the decomposition accelerating agent of the present invention contains at least one kind of in (A) and (B), foregoing extraction
Thing is the extract obtained using water as solvent.
Preferably, when decomposition accelerating agent of the invention contains (C), relative to the mass parts of glycerine 1 of foregoing (1), contain
The lactoprotein and/or yeast extract of foregoing (2) of 0.1~3 mass parts are calculated as with solid constituent.
Moreover it is preferred that the decomposition accelerating agent of the present invention is when containing (C), relative to the mass parts of glycerine 1 of foregoing (1),
The vitamin B12 of foregoing (3) containing 0.00001~0.001 mass parts.
The decomposition accelerating agent composition of the present invention is characterised by that it contains decomposition accelerating agent as active ingredient.
The method for promoting volatility organohalogen compound to decompose using microorganism of the present invention is characterised by, is made above-mentioned
Arbitrary decomposition accelerating agent or above-mentioned decomposition accelerating agent composition and the soil comprising volatility organohalogen compound and/or
Underground water contacts.
In the method for the invention, preferably foregoing volatility organohalogen compound is organochlorine based compound.
In addition, in the method for the invention, it is preferred that foregoing organochlorine compound be selected from by carbon tetrachloride, chloroform,
Dichloromethane, monochloro methane, 1,2- dichloroethanes, 1,1- dichloroethylene, cis -1,2- dichloroethylene, anti-form-1, the chloroethenes of 2- bis-
Alkene, 1,3- dichloropropylenes, tetrachloro-ethylene, 1,1,1- trichloroethanes, 1,1,2- trichloroethanes, trichloro ethylene and vinyl chloride composition
Group in it is at least one kind of.
In addition, in the method for the invention, it is preferred that aforementioned micro organism is selected from by fusobacterium (Clostridium)
Bacterium, dehalogenate Bacillus (Dehalobacter) bacterium, dehalogenation intend Coccus (Dehalococcoides) bacterium, dehalogenation spiral shell
Pseudomonas (Dehalospirilum) bacterium, Desulfobacter (Desulfobacterium) bacterium, Desulfomonas
(Desulfomonas) it is at least one kind of in the group of bacterium and desulfurization Mycotoruloides (Desulfomonile) bacterium composition.
The effect of invention
The decomposition accelerating agent of the present invention is easily processed, by being utilized by volatility organohalogen compound contaminated land
Used in the purification (biodegradation) of microorganism, contaminated soil, underground water can be made innoxious rapidly, and at a low price and to environment without
Burden.
In addition, the method for the present invention is inexpensive and free of a burden to environment, can promote by volatility organohalogen compound
Contaminated soil, the purification of groundwater use microorganism.
Brief description of the drawings
Fig. 1 is the curve map for the passage for representing c-DCE, VC, ethylene volume in embodiment 1.
Fig. 2 is the curve map for the passage for representing c-DCE, VC, ethylene volume in embodiment 2.
Fig. 3 is the curve map for the passage for representing c-DCE, VC, ethylene volume in comparative example 1.
Fig. 4 is the curve map for the passage for representing c-DCE, VC, ethylene volume in comparative example 2.
Fig. 5 is the trichloro ethylene in expression embodiment 7, the curve map of the passage of c-DCE, VC, ethylene volume.
Fig. 6 is the trichloro ethylene in expression comparative example 3, the curve map of the passage of c-DCE, VC, ethylene volume.
Fig. 7 is the trichloro ethylene in expression embodiment 15, the curve map of the passage of the amount of dichloroethylene, vinyl chloride, ethene.
Embodiment
Hereinafter, the decomposition accelerating agent of the volatility organohalogen compound of the present invention is described in detail.Need to illustrate
, in this manual, the decomposition of volatility organohalogen compound refers to the dehalogenate of volatility organohalogen compound
Change.
The decomposition accelerating agent of the volatility organohalogen compound of the present invention is characterised by that it contains following (A)~(C)
In a kind or two or more.
(A) fruit of citrus or the extract obtained by the fruit
(B) pericarp of citrus or the extract obtained by the pericarp
(C) containing the whole blend in following (1)~(3)
(1) glycerine
(2) lactoprotein and/or yeast extract
(3) vitamin B12
First, above-mentioned (A) composition and (B) composition are illustrated.
For the species of above-mentioned (A) composition and the citrus of (B) composition, it is not particularly limited, as long as to belong to
Contained plant, excellent in the plant of Rutaceae Aurantioideae, particularly Rutaceae Aurantioideae Citrus or Fortunella
Choosing is by using plant caused by the Citrus, Fortunella hybridization etc..As the concrete example of citrus, such as can enumerate：
Late logical sequence West Asia orange, navel orange, blood orange, grape fruit, lemon, shaddock, bitter orange, citrus unshiu Marcovitch, eight the first day of the lunar month, Gan Xia, pomelo, golden mandarin orange, tangerine and then
She gives mandarin orange, sees clearly, do not know tangelos such as the citruses such as fire, Seminole, bright Buddhist nun tangelo etc. caused by these hybridization etc..Wherein, calmly
The aspect that easily obtains and largely can obtain at a low price is set out, preferably using in late logical sequence West Asia orange, grape fruit, lemon, citrus unshiu Marcovitch
More than one.
Can be the whole fruit or fruit of above-mentioned citrus for above-mentioned (A) composition and (B) composition
A part, but from the high aspect of decomposition facilitation effect, preferably pericarp.
For the form of above-mentioned (A) composition and (B) composition, have no particular limits, such as can enumerate：Fruit, fruit
The form that skin, pulp directly use；Fruit, pericarp, pulp are through dry form；Fruit, pericarp, pulp are scattered in through crushing
The form of water；The form of fruit, pericarp, pulp through powdered；Fruit juice etc..In addition it is also possible to it is from fruit with water such as warm water, hot water
Real, particularly pericarp extraction extract；With non-polar solvent extracts such as ethanol, acetone, ethyl acetate polar solvent, hexanes
Extract.
Wherein, there is prompt effect when being applied to soil from the extract that pericarp extracts with water such as warm water, hot water, in addition from obtaining
Be preferable from the viewpoint of high effect.It should be noted that the temperature of water is preferably 30~100 DEG C, more preferably during extraction
For 60~95 DEG C, more preferably 60~80 DEG C.
As separator is extracted used in said extracted, as long as forming volatility of the invention effectively to obtain
The device of the extract of the decomposition accelerating agent of organohalogen compound, such as can enumerate：Continuous centrifugal device, UF membrane
Device, means of supercritical extraction device etc..
It should be noted that the pericarp of citrus is when manufacturing time processing product (fruit juice, can etc.) of citrus fruits
A large amount of by-products, most of currently without useful purposes to be dropped, the decomposition of volatility organohalogen compound of the invention
For accelerator as raw material, the decomposition accelerating agent that can not only provide volatility organohalogen compound than being currently known is cheap
Product, it is and also meaningful from effective use aspects of resource.
And then can be carried from fruit for above-mentioned (A) composition and the said extracted thing of a form of (B) composition
The extract extracted in the residue after the compositions such as pectin, fragrance component, pigment, hesperidin is taken, can also be contained beyond these
Composition.
It should be noted that the fruit of citrus is eaten since ancient times, as the volatility of the invention of raw material
The decomposition accelerating agent of organohalogen compound is also easily processed from aspect that is safe and also relatively stablizing to heat.
As above-mentioned (A) composition and (B) composition, in the fruit of citrus, particularly its pericarp plays high de-agglomeration and promoted
Effect, in addition, the effect that the extract obtained is extracted by the water of warm water, hot water etc. is high.Therefore, though detailed reason is unclear,
But as a possibility, it is believed that be the effect for playing the decomposition accelerating agent of the present invention citrus fruits in contained composition be
Water-soluble saccharides, salt, the mixture of organic acid.
Then, above-mentioned (C) composition is illustrated.
Above-mentioned (C) composition is containing the whole blend in following (1)~(3).
(1) glycerine
(2) lactoprotein and/or yeast extract
(3) vitamin B12
The glycerine used in the blend of above-mentioned (C) composition is the carbon source of microorganism, and as substituting organochlorine
The supply source of the hydrogen of the chlorine atom of based compound, i.e. hydrogen donor, glycerine can be used in itself, or be combined with 1~3 fat
The form of the glyceride of fat acid, preferably using glycerine in itself.During using commercially available glycerine, be not limited to purity for 100%,
More than 99% glycerine (for example, reagent is superfine), glycerine (purity 80~90%), the purification of glycerol of Japanese Pharmacopoeia can also be used
D, food additives glycerine, concentrated glycerin used for cosmetic (being Lion Corporation. manufactures) etc..
In the blend of above-mentioned (C) composition, the nitrogen source of microorganism is used as using lactoprotein and/or yeast extract.
As above-mentioned lactoprotein, it can be only lactalbumin, be only casein, casein and lactalbumin is applied in combination
Any of, but lactalbumin and casein is more preferably applied in combination.
In addition, above-mentioned lactoprotein is preferably water solubility.During using commercially available lactoprotein, as long as to contain newborn egg with high concentration
White product and food (or will not significantly hinder what microorganism grew with, the harmless lactoprotein such as used for cosmetic
Lactoprotein), such as can enumerate：Casein-sodium, potassium caseinate, whey powder, WPC (whey protein concentrate), WPI (breasts
Albumin isolate), total milk protein (TMP), protein compression whey powder, full milk powder, skimmed milk power, Reduced lactose whey, demulsification
Sugared whey powder, buttermilk powder, sweetened powdered milk, modified milk powder, milk protein concentrate (MPC) etc..In the present invention, from lipid content it is low,
And the high aspect of storage stability is set out, preferably total milk protein (TMP) and/or skimmed milk power, more preferably skimmed milk power.
Above-mentioned yeast extract refer to by the culture of yeast is carried out self-dissolving, enzyme, hot water, physics is broken, acid decomposes,
The processing such as caustic digestion, freezing fusion method are so as to the extract of extraction.To the species of the yeast used in the manufacture of yeast extract
It is not particularly limited, Saccharomyces cerevisiae, brewer's yeast, wine yeast, torula etc. can be used without particular limitation.Its
In, preferably using the yeast for belonging to saccharomyces (Saccharomyces).Yeast extract can be pasty state, powdered, graininess
Any of.
In the above-mentioned lactoprotein and yeast extract of the present invention, above-mentioned lactoprotein can be used only, in addition can also be only
Using yeast extract, but lactoprotein is preferably used only, lactoprotein and yeast extract further preferably is applied in combination.
Mixing ratio when wherein, for being applied in combination, relative to the mass parts of lactoprotein 1, make yeast extract with solid into
It is preferably 0.1~2 mass parts, more preferably 0.3~1 mass parts to divide meter.
In the blend of above-mentioned (C) composition, the lactoprotein of glycerine, (2) for above-mentioned (1) and/or yeast extract
Content ratio, relative to the mass parts of glycerine 1 of above-mentioned (1), make above-mentioned (2) lactoprotein and/or yeast extract with solid constituent
Meter is preferably 0.1~3 mass parts, more preferably 0.1~1 mass parts.
In the blend of above-mentioned (C) composition, vitamin B12 is used.
Vitamin B12 is the general name of the vitamin containing cobalt, is one kind of water soluble vitamin, there is hydroxocobalamine, adenosine
Cobalamin, methyl cobalamin, cyanocobalamin, sulphitocobalamine etc., it can use its any one in the present invention.
It can be used to purify product in the present invention, in addition it is also possible to use the largely food containing vitamin B12.For example,
Vitamin B12 mostly contain sea sedge, shellfish, animal food liver in.
In the blend of above-mentioned (C) composition, the content ratio of the vitamin B12 of glycerine, (3) for above-mentioned (1), relative to
The mass parts of glycerine 1 of above-mentioned (1), the vitamin B12 for making above-mentioned (3) are preferably 0.00001~0.001 mass parts, are more preferably
0.00002~0.0001 mass parts.
The present invention volatility organohalogen compound decomposition accelerating agent can contain except above-mentioned (A) composition, (B) into
Divide, the other compositions beyond (C) composition.
As above-mentioned other compositions, for example, can be with glucose, fructose, ammonium sulfate, the urine of the nutrient source as microorganism
The magnesium compound such as the potassium compounds such as element, ammonium salt, sulphur compound, phosphorus compound, potassium chloride, magnesium chloride, magnesium sulfate, yeast extraction
Thing or peptone etc. are used together.In addition, relative to the present invention decomposition accelerating agent, can also be formed with the addition of it is proper amount of
The decomposition accelerating agent composition of above-mentioned additive.When forming decomposition accelerating agent composition, do not have to the compounding amount of each additive
Special limitation, for example, during using powder yeast extract, fructose, relative to the mass of solid constituent 100 of the extract of fruit
Part, it is preferably each 1~200 mass parts, more preferably 10~100 mass parts in terms of solid constituent.
Wherein, when using (C) composition as the present invention, as above-mentioned other compositions, microorganism can be turned into by preferably not containing
Nutritional ingredient composition, such as：Glucose, fructose, ammonium sulfate, urea, ammonium salt, sulphur compound, phosphorus compound, potassium chloride etc.
The magnesium compounds such as potassium compound, magnesium chloride, magnesium sulfate or peptone etc..Particularly sulfate reduction be present in soil, underground water
During bacterium, the lower and sulfatereducting bacteria coexists in sulfate ion and competes, therefore becomes that volatility organohalogen compound can not be carried out
Decomposition, therefore preferably do not include the sulfate such as ammonium sulfate, magnesium sulfate.
The form of decomposition accelerating agent of the volatility organohalogen compound of the present invention is not particularly limited, can be with
Using various forms such as solid (including powdered, graininess), liquid (including pasty state).In addition it is also possible to molten using water etc.
Agent uses in the state of being diluted.
The present invention decomposition accelerating agent by with by volatility organohalogen compound contaminated soil, underground water, other
Sample contacts, the volatility organohalogen compound is promoted to utilize the decomposition of microorganism.As the volatility of the object of the present invention
Organohalogen compound is preferably organochlorine based compound, such as can be enumerated：Carbon tetrachloride, chloroform, dichloromethane, a chloromethane
Alkane, 1,2- dichloroethanes, 1,1- dichloroethylene, cis -1,2- dichloroethylene, anti-form-1,2- dichloroethylene, 1,3- dichloros third
Alkene, tetrachloro-ethylene, 1,1,1- trichloroethanes, 1,1,2- trichloroethanes, trichloro ethylene, vinyl chloride etc..
Wherein, decomposition accelerating agent of the invention can suitably promote tetrachloro-ethylene, trichloro ethylene, dichloroethylene class, chlorine
The decomposition of the vinyl chloride class such as ethene.
For example, tetrachloro-ethylene is broken down into trichloro ethylene, dichloroethylene, a vinyl chloride (chloroethene by microorganism successively
Alkene), ethene.
The decomposition accelerating agent of the present invention can utilize to promote volatility organohalogen compound to utilize the decomposition of microorganism
As microorganism existing for script in the soil of purification object, underground water, can also use simultaneously to volatility organic halogen
Compound decomposes useful microorganism.In addition it is also possible to the composition comprising mentioned microorganism is used simultaneously.That is, as net
It can make the present invention's when changing the microorganism for fully including decomposing volatile organohalogen compound in the soil of object, underground water
Decomposition accelerating agent, decomposition accelerating agent composition directly apply to object soil.On the other hand, the microbial biomass in soil is less
When, or during hope quickening decomposition, can be used together the present invention's with pre-prepd microorganism, the composition comprising microorganism
Decomposition accelerating agent, decomposition accelerating agent composition.
The microorganism useful as the decomposition to volatility organohalogen compound, preferably anerobe, such as
It can enumerate：Fusobacterium, dehalogenate Bacillus, dehalogenation intend Coccus, dehalogenation Spirillum, Desulfobacter, Desulfomonas,
The microorganisms such as desulfurization Mycotoruloides.
During using decomposition accelerating agent of the invention, preferably measured in advance is included in the sample of volatility organohalogen compound
Anerobe, for example, dehalogenation intend Coccus bacterium amount.It is real that dehalogenation intends can quantitatively utilizing for Coccus bacterium
When method known to PCR methods etc. (for example, with reference to non-patent literature 1).
For making the decomposition accelerating agent of the present invention be not particularly limited with the method that soil and/or underground water contact,
Can remove contaminated soil, underground water, " processing of facility type " handled in other places or at this
Place purifies " the original position purification " of contaminated soil, underground water, but from micro- life of decomposing volatile organohalogen compound
Thing plays the effect and set out to greatest extent under conditions of anaerobism, and preferably original position purifies.
Facility type purify in the case of, for make the present invention decomposition accelerating agent and exist microorganism soil and/or
The method of underground water contact is not particularly limited, such as can be enumerated：The contaminated soil through excavation is accumulated, thereto directly
The method of injection；The method mixed with contaminated soil；Add water in contaminated soil and entered in the form of flowing shape~liquid
Method of row addition etc..
In the original location purify in the case of, for make the present invention decomposition accelerating agent and exist microorganism soil and/or
The method of underground water contact is not particularly limited, such as can be：The method for being directly embedded in soil；Noted using injection well
Enter to the direct injection in underground water or soil；Use the side of the permeability reaction purification wall of the flowing using underground water
Method, but preferably direct injection.
It should be noted that as long as the quantity delivered of the decomposition accelerating agent of the present invention is can obtain sufficient clean-up effect
Degree, the scope of Polluted area, the degree of pollution, species of polluter etc. are determined by advance pretesting to determine
.
Embodiment
<The decomposition experiment 1 of vinyl chloride class>
(manufacture 1 of decomposition accelerating agent)
<The manufacture of citrus extract>
(Production Example 1)
Fully after washing citrus unshiu Marcovitch, peeling, after crushing pericarp (dry weight 100g) using disc flour mill, with 60 DEG C
Warm water 2000ml stirring extraction 1 hour.Filtered, after filtrate is using rotary evaporator concentration, entered using vacuum drier
Row drying, so as to obtain the pericarp warm water extract (about 10g) of citrus unshiu Marcovitch.By the pericarp warm water extract of acquisition directly as
The decomposition accelerating agent A of the present invention.
(Production Example 2)
Relative to the mass parts of pericarp warm water extract 100 obtained in Production Example 1, the mass of addition powder yeast extract 50
Part and the mass parts of fructose 50 and mix in heterogeneity, as the decomposition accelerating agent B of the present invention.
(Production Example 3)
Using 60 DEG C of warm water used in 98 DEG C of hot water replacement extractions, in addition, this hair is obtained in the same manner as Production Example 1
Bright decomposition accelerating agent C.
(Production Example 4)
Citrus unshiu Marcovitch is replaced using late logical sequence West Asia orange, in addition, the decomposition that the present invention is obtained in the same manner as Production Example 1 promotees
Enter agent D.
(Production Example 5)
Citrus unshiu Marcovitch is replaced using grape fruit, in addition, the decomposition accelerating agent of the present invention is obtained in the same manner as Production Example 1
E。
(Production Example 6)
Citrus unshiu Marcovitch is replaced using lemon, in addition, the decomposition accelerating agent F of the present invention is obtained in the same manner as Production Example 1.
(evaluation method 1 of decomposition accelerating agent)
<The making of bacterium liquid>
(on decomposition accelerating agent A~F)
The culture medium of yeast extract will be added in a manner of 0.1g/L in mineral matter basal medium as follows
50mL is taken to 100mL volumetric glass bottles, after nitrogen is replaced, after carrying out sterilization treatment using autoclave, is added from by ethene
The underground water 25mL that base chlorine class contaminated soil is taken, after nitrogen is replaced, enclose hydrogen 2.5mL and cis-chloroethenes of 1,2- bis-
The μ L (equivalent to 10mg/L) of alkene 0.58, the dark place quiescent culture at 20 DEG C.Vinyl chloride class in results of regular determination headroom,
1mL is taken at the time of not detecting vinyl chloride class, continues to be seeded to the ore deposit that addition yeast extract 0.1g/L autoclaving is crossed
In material base culture medium 75mL.Material that this cultivates 6 times again will be carried out as " bacterium liquid ", for following vinyl chloride classes
Decomposition experiment.
<The manufacture of mineral matter basal medium (pH7.0~7.5)>
By following salt storing solution 10ml, following trace element solution A 1ml, following trace element solution B1ml, resazurin
μ l of sodium solution (0.5%w/v) 50, sodium acetate 0.1g, L-cysteine hydrochloride monohydrate 0.3g, sodium acid carbonate 2.52g, sulphur
Change sodium nonahydrate 0.048g and be filled up to 1000ml, as mineral matter basal medium.
<The manufacture of salt storing solution>
Following compositions water is dissolved and is filled up to 1000ml, as salt storing solution.
100g NaCl、50g MgCl2·6H2O、20g KH2PO4、30g NH4Cl、30g KCl、1.5g CaCl2·2H2O
<Trace element solution A manufacture>
Following compositions water is dissolved and is filled up to 1000ml, as trace element solution A.
10mL HCl (25% solution, w/w), 1.5g FeCl2·4H2O、0.19g CoCl2·6H2O、0.1g MnCl2·
4H2O、70mg ZnCl2、6mg H3BO3、36mg Na2MoO4·2H2O、24mg NiCl2·6H2O、2mg CuCl2·2H2O
<Trace element solution B manufacture>
Following compositions water is dissolved and is filled up to 1000ml, as trace element solution B.
6mg Na2SeO3·5H2O、8mg Na2WO4·2H2O、0.5g NaOH
<The decomposition experiment 1 of vinyl chloride class>
(embodiment 1)
It is assumed that the underground water polluted by vinyl chloride class, decomposition run is carried out by following methods.
Above-mentioned mineral matter basal medium 75ml is taken into the bottle of glass system 100ml capacity, in a manner of 0.1g/L
Above-mentioned decomposition accelerating agent A is added, sterilization treatment is carried out with autoclave after nitrogen is replaced.After cooling, above-mentioned bacterium liquid is added
1.5ml, after nitrogen is replaced, cis -1,2-dichloroethene (c-DCE) is enclosed in a manner of 10 μ g/ml.
The bottle is subjected to quiescent culture at 20 DEG C.0 day, 3 days, 10 days, 18 days, 24 days, 36 days, 45 days, 49 days, 59
My god, 66 days, 75 days, 84 days, after 87 days, pass through gas chromatography determine bottle headroom in cis-chloroethenes of 1,2- bis-
Alkene (c-DCE) content, vinyl chloride (VC) content, ethylene contents.
On experimental result, first, figure 1 illustrates the cis -1,2-dichloroethene (c-DCE) of the 0th day~the 87th day
The growth and decline of content, vinyl chloride (VC) content, ethylene contents.
Further, it is contemplated that the activation of microorganism is initial caused, therefore every 1 day of the c-DCE contents of near 18th day
Decrement is shown in table 1 as initial decomposition speed.
(embodiment 2)
Decomposition accelerating agent B is added in a manner of 0.2g/L and replaces decomposition accelerating agent A 0.1g/L, in addition, with embodiment
1 similarly carries out the decomposition experiment of vinyl chloride class, is as a result recorded in Fig. 2 and table 1.
(comparative example 1)
Decomposition accelerating agent A 0.1g/L are not added, in addition, carry out the decomposition of vinyl chloride class similarly to Example 1
Experiment, is as a result recorded in Fig. 3 and table 1.
(comparative example 2)
Decomposition accelerating agent B 0.2g/L are not added, and add yeast extract 0.05g/L and fructose 0.05g/L, except this
Outside, the decomposition experiment of vinyl chloride class is carried out similarly to Example 2, is as a result recorded in Fig. 4 and table 1.
(embodiment 3)
Decomposition accelerating agent C is added in a manner of 0.1g/L and replaces decomposition accelerating agent A 0.1g/L, in addition, with embodiment
1 similarly carries out the decomposition experiment of vinyl chloride class.Wherein, sampling is set to the 0th day, the 3rd day, the 10th day, the 18th day, the 18th day
Untill, the decrement of every 1 day of content to the c-DCE of the 18th day is calculated similarly to Example 1 as initial decomposition speed
It is shown in table 1.
(embodiment 4)
Decomposition accelerating agent D is added in a manner of 0.1g/L and replaces decomposition accelerating agent A0.1g/L, in addition, with embodiment 1
Similarly carry out the decomposition experiment of vinyl chloride class.In addition, during sampling is set to similarly to Example 3, initial point is only calculated
Speed is solved, is as a result recorded in table 1.
(embodiment 5)
Decomposition accelerating agent E is added in a manner of 0.1g/L and replaces decomposition accelerating agent A0.1g/L, in addition, with embodiment 1
Similarly carry out the decomposition experiment of vinyl chloride class.In addition, during sampling is set to similarly to Example 3, initial point is only calculated
Speed is solved, is as a result recorded in table 1.
(embodiment 6)
Decomposition accelerating agent F is added in a manner of 0.1g/L and replaces decomposition accelerating agent A 0.1g/L, in addition, with embodiment
1 similarly carries out the decomposition experiment of vinyl chloride class.In addition, during sampling is set to similarly to Example 3, initial point is only calculated
Speed is solved, is as a result recorded in table 1.
[table 1]
※ units=(μ g/l/ days)
By the result (Fig. 3) of comparative example 1 it will be apparent that, even if still residual by the 87th day in the case of only minimal medium
Stay c-DCE, VC also to have rising trend, do not find to produce the situation of ethene, the decomposition of vinyl chloride class is not almost carried out.
On the other hand, by embodiment 1 (Fig. 1), the result (Fig. 2) of embodiment 2 it will be apparent that, for the addition of the present invention
The sample of decomposition accelerating agent, almost it is decomposed in the 50th day c-DCE, is all almost decomposed completely in the 87th day c-DCE, VC.
It should be noted that comparing embodiment 2 (Fig. 2) and comparative example 2 (Fig. 4) it will be apparent that, relative to using current
Nutritional agents is the sample of yeast extract and fructose, and decomposition accelerating agent of the invention is added by additional in the nutritional ingredient,
So that c-DCE is more quickly decomposed, particularly the decomposition rate at the 18th~50 day significantly rises.Same display VC maximums are dense
The date of degree became the 24th day (embodiment 2, Fig. 2) from the 59th day (comparative example 2, Fig. 4), thus it is confirmed that：By current
Nutritional agents in the additional addition present invention decomposition accelerating agent, initial decomposition rate can be increased substantially.
In addition, by table 1 it will be apparent that, by add the present invention decomposition accelerating agent, initial decomposition rate significantly on
Rise.
It should be noted that, although also there is certain point using the sample that current nutritional agents is yeast extract and fructose
Solve speed improve effect, but comparing embodiment 1,3~6 and comparative example 2 it will be apparent that, using the present invention decomposition accelerating agent when,
It is high to decompose facilitation effect.And then from the result of embodiment 2, by the additional decomposition accelerating agent for adding the present invention, initial
Decomposition rate significantly rises.
(embodiment 7 and comparative example 3)
It is assumed that by vinyl chloride class contaminated land, decomposition run is carried out by following methods.
In 1L capacity screw top jars, it is put into from the soil 700g taken by trichloro ethylene contaminated land, from identical field
The underground water 300g taken, after adding above-mentioned bacterium liquid 5mL, decomposition accelerating agent A0.2g and yeast extract 0.2g is dissolved
Added after 50mL distilled water, after nitrogen is replaced, carry out quiescent culture at room temperature in the dark.Periodically determined by gas chromatography
The concentration of vinyl chloride class (trichloro ethylene, c-DCE, VC) and the concentration of ethene in headroom.With Polluted Soil and ground
The vinyl chloride class headed by trichloro ethylene included in lower water all became environmental benchmark value (trichloro ethylene after the 120th day
=0.03mg/l, dichloroethylene=0.04mg/l, VC=0.002mg/l) below.As a result it is shown in Fig. 5.Wherein, fructose is added
0.2g replace decomposition accelerating agent A in the case of (comparative example 3), even across 150 days VC still with groundwater environment a reference value more than
Amount residual, it is impossible to purify soil.As a result it is shown in Fig. 6.
<The decomposition experiment 2 of vinyl chloride class>
(manufacture 2 of decomposition accelerating agent)
(Production Example 7~16)
Extracted according to the record mixing of table 2 as the glycerine, the skimmed milk power as (2) composition and/or yeast of (1) composition
Thing powder, the Vitamin B12 preparation as (3) composition, obtain decomposition accelerating agent G~P.It should be noted that the decomposition obtained
In accelerator G~N and P be pasty state, O be powdered.
It should be noted that on (1):(2) content ratio and (1):(3) content ratio is also recorded in table 2.
On decomposition accelerating agent G~P of acquisition, evaluated according to the evaluation method of following decomposition accelerating agents, as a result remembered
It is loaded in table 2.
(evaluation method 2 of decomposition accelerating agent)
<The making of bacterium liquid>
The culture medium 50mL for adding yeast extract in a manner of 0.1g/L in above-mentioned mineral matter basal medium is taken
To 100mL volumetric glass bottles, after nitrogen is replaced, after carrying out sterilization treatment using autoclave, add from by vinyl chloride class
The underground water 25mL that contaminated soil is taken, after nitrogen is replaced, enclose hydrogen 2.5mL and cis-μ L of 1,2-dichloroethene 0.58
(equivalent to 10mg/L), the dark place quiescent culture at 20 DEG C.Vinyl chloride class in results of regular determination headroom, is not detecting second
1mL is taken at the time of alkenyl chlorine class, continues to be seeded to the mineral matter basis that addition yeast extract 0.1g/L autoclaving is crossed
In culture medium 75mL.Material that this cultivates 6 times (wherein only the 6th time is no added yeast extract) again will be carried out as " thin
Bacterium solution ", the decomposition experiment for following vinyl chloride classes.
<The decomposition experiment 2 of vinyl chloride class>
(embodiment 8~14, comparative example 4~6)
It is assumed that the underground water polluted by vinyl chloride class, decomposition run is carried out by following methods.
Above-mentioned mineral matter basal medium 75ml is taken into the bottle of glass system 100ml capacity, with respective 0.3g/L's
Mode adds above-mentioned decomposition accelerating agent G~P, and sterilization treatment is carried out with autoclave after nitrogen is replaced.After cooling, above-mentioned bacterium is added
Liquid 1.5ml, after nitrogen is replaced, cis -1,2-dichloroethene (c-DCE) is enclosed in a manner of 10 μ g/ml.
The bottle is subjected to quiescent culture at 20 DEG C.In the headroom that bottle is periodically determined by gas chromatography
C-DCE contents, VC contents, ethylene contents.On experimental result, by c-DCE contents and VC contents until being changed into underground water ring
Below a reference value of border, i.e. c-DCE is changed into below 0.04mg/L and number of days at the time of VC is changed into below 0.002mg/L is as " extremely
Number of days required for decomposing ", is recorded in table 2.
It should be noted that the situation of no added decomposition accelerating agent is similarly tested as comparative example 7, as a result record
In table 2.
[table 2]
When as shown in Table 2, using the decomposition accelerating agent of the embodiment 8~14 containing (1)~(3) composition, to second
The number of days that alkenyl chlorine class is decomposed completely is less than 31 days, in contrast, the comparative example 4 for not containing (1) composition is 48 days, is not contained
(2) comparative example 5 of composition is 45 days, and the comparative example 6 for not containing (3) composition is 40 days, learns that decomposition rate is extremely low.
It should be noted that without using (comparative example 7) during decomposition accelerating agent even in the 100th day vinyl chloride class decomposition
Also do not complete.
Use the embodiment 9,11,12 of skimmed milk power and/or yeast extract to understand as (2) composition in addition, comparing, make
It is higher than the embodiment 11 using only yeast extract with the decomposition rate of the embodiment 9 of skimmed milk power, skimmed milk power is applied in combination
With the decomposition rate highest of the embodiment 12 of yeast extract.
It should be noted that the embodiment 9,13,14 for comparing the content of various changes vitamin B12 is understood, vitamin
Decomposition rate will not if in the range of 0.00001~0.001 mass parts relative to the compounding amount of the mass parts of glycerine 1 by B12
Produce difference.
(embodiment 15)
It is assumed that by vinyl chloride class contaminated land, decomposition run is carried out by following methods.
In 1L capacity screw top jars, it is put into from the soil 700g taken by trichloro ethylene contaminated land, from identical field
The underground water 300g taken, after adding above-mentioned bacterium liquid 5mL, add after decomposition accelerating agent E 0.5g are dissolved in into 50mL distilled water
Enter, after nitrogen is replaced, carry out quiescent culture at room temperature in the dark.The ethene in headroom is periodically determined by gas chromatography
The concentration of base chlorine class (trichloro ethylene, c-DCE, VC) and the concentration of ethene.With three included in Polluted Soil and underground water
Vinyl chloride class headed by vinyl chloride was all changed into environmental benchmark value (trichloro ethylene=0.03mg/l, two chloroethenes after the 150th day
Alkene=0.04mg/l, VC=0.002mg/l) below.As a result it is shown in Fig. 7.
<The decomposition experiment 3 of vinyl chloride class>
(evaluation method 3 of decomposition accelerating agent)
<The making of bacterium liquid>
The culture medium 50mL for adding yeast extract in a manner of 0.1g/L in above-mentioned mineral matter basal medium is taken
To 100mL volumetric glass bottles, after nitrogen is replaced, after carrying out sterilization treatment using autoclave, add from by tetrachloro-ethylene
(PCE) the underground water 25mL that contaminated soil is taken, after nitrogen is replaced, the μ L of inclosure hydrogen 2.5mL and PCE 0.46 (equivalent to
10mg/L), the dark place quiescent culture at 20 DEG C.Vinyl chloride class in results of regular determination headroom, is not detecting vinyl chloride
1mL is taken at the time of class, continues to be seeded to the mineral matter basal medium that addition yeast extract 0.1g/L autoclaving is crossed
In 75mL.Material that this cultivates 3 times again will be carried out as " bacterium liquid ", the decomposition experiment for following vinyl chloride classes.
(embodiment 16~18)
It is assumed that the underground water polluted by vinyl chloride class, decomposition run is carried out by following methods.
Above-mentioned mineral matter basal medium 75ml is taken into the bottle of glass system 100ml capacity, respectively with 0.2g/L's
Mode add above-mentioned decomposition accelerating agent A (embodiment 16), above-mentioned decomposition accelerating agent G (embodiment 17), above-mentioned decomposition accelerating agent A with
Above-mentioned decomposition accelerating agent G equal amount of mixture (embodiment 18), after nitrogen is replaced, sterilization treatment is carried out using autoclave.Cooling
Afterwards, above-mentioned bacterium liquid 1.5ml is added, after nitrogen is replaced, tetrachloro-ethylene (PCE) is enclosed in a manner of 10 μ g/ml.
The bottle is subjected to quiescent culture at 20 DEG C.0 day, 3 days, 10 days, 18 days, 24 days, 36 days, 45 days, 49 days,
After 59 days, 66 days, 75 days, 84 days, 87 days, the various vinyl chlorides in the headroom of bottle are determined by gas chromatography
Class, i.e. PCE, TCE, c-DCE, t-DCE, 1,1-DCE, VC content and ethylene contents.
For experimental result, by the content of vinyl chloride class until being changed into below groundwater environment a reference value, i.e., PCE is changed into
Below 0.01mg/L, TCE are changed into that below 0.03mg/L, cDCE and t-DCE are changed into below 0.04mg/L, 1,1-DCE is changed into
Number of days at the time of below 0.02mg/L, VC are changed into below 0.002mg/L is shown in table 3 as " number of days to required for decomposing ".
Further, it is contemplated that the activation of microorganism is initially occurring, therefore the reduction of every 1 day of the PCE contents of near 18th day
Amount is shown in table 3 as initial decomposition speed.
[table 3]
Claims (5)
1. a kind of decomposition accelerating agent of volatility organohalogen compound, it is characterised in that it contains 1 in following (B) and (C)
Kind or 2 kinds, and at least contain (C),
(B) extract obtained by the pericarp of citrus, the extract are to be obtained by 30~100 DEG C of water of temperature as solvent
The extract obtained
(C) containing the whole blend in following (1)~(3)
(1) glycerine
(2) lactoprotein of 0.1~3 mass parts is calculated as with solid constituent relative to the mass parts of glycerine 1 of (1)
(3) vitamin B12 relative to the mass parts of glycerine 1 of (1) for 0.00001~0.001 mass parts,
Volatility organohalogen compound is organochlorine based compound.
2. a kind of decomposition accelerating agent composition of volatility organohalogen compound, it is characterised in that it contains claim 1 institute
The decomposition accelerating agent stated is as active ingredient.
A kind of 3. method for promoting volatility organohalogen compound to decompose using microorganism, it is characterised in that make claim 1
Decomposition accelerating agent composition described in described decomposition accelerating agent or claim 2 is with including volatility organohalogen compound
Soil and/or underground water contact.
4. according to the method for claim 3, wherein, organochlorine based compound is selected from by carbon tetrachloride, chloroform, dichloromethane
Alkane, monochloro methane, 1,2- dichloroethanes, 1,1- dichloroethylene, cis -1,2- dichloroethylene, anti-form-1,2- dichloroethylene, 1,
In the group that 3- dichloropropylenes, tetrachloro-ethylene, 1,1,1- trichloroethanes, 1,1,2- trichloroethanes, trichloro ethylene, vinyl chloride form
It is at least one kind of.
5. according to the method for claim 3, wherein, microorganism be selected from by Clostridia bacteria, dehalogenate Bacillus bacteria,
Dehalogenation intends Coccus bacterium, dehalogenation Spirillum bacterium, Desulfobacter bacterium, Desulfomonas bacterium, desulfurization Mycotoruloides
It is at least one kind of in the group of bacterium composition.
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| JP2012-095109 | 2012-04-18 | ||
| PCT/JP2013/061334 WO2013157556A1 (en) | 2012-04-18 | 2013-04-16 | Decomposition accelerator for volatile organic halogen compound |
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| CN106587342A (en) * | 2016-12-29 | 2017-04-26 | 中国科学院城市环境研究所 | Bioremediation reagent and application thereof in removing 1,2-dichloroethane in composite polluted water body |
| JP7049612B2 (en) * | 2017-02-17 | 2022-04-07 | シナプテック株式会社 | Manufacturing method of VOC decomposition accelerator for soil improvement |
| JP2018175529A (en) * | 2017-04-17 | 2018-11-15 | 東急建設株式会社 | Decomposition accelerator, and environmental cleanup method using the decomposition accelerator |
| TWI774052B (en) * | 2020-09-08 | 2022-08-11 | 財團法人工業技術研究院 | Novel dehalococcoides sp., mixed consortium having a dechlorination effect, microbial preparation containing the mixed consortium, dechlorination method for chlorinated hydrocarbons and bioremediation method by the microbial preparation |
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| US5578210A (en) * | 1994-11-15 | 1996-11-26 | The Dow Chemical Company | Method for stimulating anaerobic biotransformation of halogenated hydrocarbons |
| US5968360A (en) * | 1997-05-27 | 1999-10-19 | The Regents Of The University Of California | Composition and method for degradation of polychlorinated biphenyl compounds |
| WO1999024367A1 (en) * | 1997-11-12 | 1999-05-20 | Regenesis Bioremediation Products | Polylactate release compounds and methods of using same |
| EP1238718A4 (en) * | 1999-11-11 | 2004-09-01 | Idemitsu Kosan Co | Method of degrading hardly degradable harmful material |
| JP2002153257A (en) * | 2000-11-20 | 2002-05-28 | Idemitsu Kosan Co Ltd | Microorganism bed mat and method for cleaning soil polluted with persistent injurious substance with the mat |
| JP3538643B1 (en) * | 2003-03-03 | 2004-06-14 | エコサイクル株式会社 | Additives used to remediate contaminated soil, groundwater or sediment |
| US7381337B2 (en) * | 2003-07-17 | 2008-06-03 | Lessard Environmental, Inc. | Gel-based remedial additive for remediation of environmental media and method of use |
| JP2005087980A (en) * | 2003-09-12 | 2005-04-07 | Toru Ueda | Purification method of dioxins-containing soil using fruit juice or fruit juice waste |
| JP2005288276A (en) * | 2004-03-31 | 2005-10-20 | Ecocycle Corp | Additive used in restoring contaminated soil, ground water or sedimentary soil deposit |
| JP5218807B2 (en) * | 2007-05-25 | 2013-06-26 | アイシン精機株式会社 | Culture composition |
| US8105808B2 (en) * | 2007-06-13 | 2012-01-31 | The United States Of America As Represented By The Secretary Of The Interior | Anaerobic microbial composition and methods of using same |
| JP5163235B2 (en) * | 2008-03-31 | 2013-03-13 | 株式会社大林組 | In-situ purification method for contaminated ground or groundwater |
| JP4729758B2 (en) * | 2008-10-31 | 2011-07-20 | Ａｄｅｋａ総合設備株式会社 | Decomposition promoter and method for promoting degradation of volatile organic halogen compounds by microorganisms |
| TWI373523B (en) * | 2008-12-05 | 2012-10-01 | Taiwan Agricultural Chemicals And Toxic Substances Res Inst Council Of Agriculture | Novel strain of bacillus amyloliquefaciens and its use |
| JP5303389B2 (en) * | 2009-07-23 | 2013-10-02 | Ｊｆｅミネラル株式会社 | Soil or groundwater purification agent and purification method |
| WO2011148509A1 (en) * | 2010-05-28 | 2011-12-01 | エコサイクル株式会社 | Agent and method for purifying medium contaminated with organic chlorine compound |
| CN101948171B (en) * | 2010-08-20 | 2012-10-03 | 北京大学 | Permeable reaction wall method capable of repairing halohydrocarbon and nitrate polluted groundwater |
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