CN104404101B - The preparation method of lotion welan gum - Google Patents
The preparation method of lotion welan gum Download PDFInfo
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- CN104404101B CN104404101B CN201410631253.9A CN201410631253A CN104404101B CN 104404101 B CN104404101 B CN 104404101B CN 201410631253 A CN201410631253 A CN 201410631253A CN 104404101 B CN104404101 B CN 104404101B
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Abstract
The invention belongs to the preparation of microbial polysaccharide solution, particularly a kind of preparation method of lotion welan gum.Including Sphingol single-cell CGMCCNo.1561 is inoculated in containing carbon source, nitrogen source, inorganic salts and water without being fermented in bacteria fermentation culture medium, extraction, separation of solid and liquid, produce the processing steps such as lotion welan gum, the present invention solves powdered welan gum existing for prior art and cannot be directly used to oil-base mud in oil drilling when in use, the technical barriers such as processing safety difference, it is simple with method, product is suitable to industrialized production, transport and preservation, stability is good and can be quickly uniform with oil-base mud mixture, non-dusting during use, the advantages that being advantageous to environmental protection and reducing material loss.
Description
Technical field
The invention belongs to the preparation of microbial polysaccharide, particularly a kind of preparation method of lotion welan gum.
Background technology
Welan gum (WELAN GUM) is a kind of technical grade microbial polysaccharide, and it contains neutral sugar, D-Glucose, D=grapes
The acetyl ester group that aldehydic acid, L- rhamnoses and L=mannoses and glycosidic inkage close.Jansson PE, LindbergB and Widmalm
G (1985) describes the structure of the polysaccharide, Patent No. in " carbohydrate compound research " (139,217-223)
Disclose a kind of preparation method of welan gum in 200610012511.0 patent of invention, current welan gum be solid powder and
With good heat resistance, its aqueous solution has good viscosity and rheological property, is resistant to the calcium ion of high concentration, is used for
Cement industry can be used as cement additive, increase the denseness and rheological behavior of ore in sand form, improve the stability of concrete self-compaction, increase
The resistance to compression bleeding of big slurry concrete.Available for petroleum drilling and mining industry, thickening, suspension and rheological behavior are provided for oil mud,
It can be additionally used in the industries such as fine chemistry industry, daily use chemicals.Welan gum in the wild or operation on the sea using when powder easily fly upward, loss amount it is big and
The formation solution after work top meets water that descends slowly and lightly makes workbench become cunning, and security is poor when operating personnel operate；And powdered temperature wheel
Dissolving not exclusively, is easily lumpd when glue is directly added into oil-base mud in oil drilling when in use, influences service efficiency；Other powder
Shape welan gum also there is using process it is relatively complicated, not easy to operate the problems such as.
The content of the invention
It is an object of the invention to provide a kind of preparation method of lotion welan gum, prepared welan gum surely without dust,
Dissolution velocity is fast.
The overall technology of the present invention is conceived：
The preparation method of lotion welan gum, including by Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561
It is inoculated in containing carbon source, nitrogen source, inorganic salts and water without being fermented in bacteria fermentation culture medium；It is further comprising the steps of：
A, extract
To being extracted in the zymotic fluid after fermentation ends, extractant makes to mix from one kind in ethanol or ethanol solution
The concentration of alcohol closed in liquid is 70%-75%；
B, separation of solid and liquid
Separation of solid and liquid is carried out to mixed material and obtains solid material；
C, lotion welan gum is produced
Solid material adds water to be configured to lotion welan gum through colloid mill.
Extractant selects ethanol or ethanol solution, and main function is to play anti-corrosion and environmental-protection function, while after ensureing extraction
Material do not stick together phenomenon, be easy to easily operated when Jia Shui produces paste welan gum.
Preferable technical scheme is that the extraction time in described step A is not less than 30 minutes.
The technical scheme being more highly preferred to is that the extraction time in described step A is 30-40 minutes.
Further to reach the antiseptic effect of material after extraction, preferable technical scheme is, will fermentation in described step A
Zymotic fluid after end maintains 5-10 minutes after being warming up to 80-90 DEG C, is extracted after being cooled to 50-60 DEG C.
For ease of the preparation of lotion welan gum, preferable technical scheme is that the solid material in described step B, C is aqueous
Measure as 30%-35%.
Solid content in described step C lotion welan gum is 3%-3.5%.
To reduce the particulate material in fermentation medium, the impurity in solid material is remained in after ensureing fermentation, extraction
Few, preferable technical scheme is that the nitrogen source of described fermentation medium selects diammonium hydrogen phosphate.
The few requirement of material impurity after being fermented for further satisfaction, while ensure that the product that is normally carried out and improves of fermentation is received
The needs of rate.Preferable technical scheme is that described fermentation medium is made up of the component of weight/mass percentage composition：
Glucose 6%-6.5%；Diammonium hydrogen phosphate 0.3%-0.5%；Dipotassium hydrogen phosphate 0.2%-0.3%；Surplus is water；
PH=6.5-7.5.
Described fermentation condition is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours；It is fermentation broth viscosity not regrowth to put tank standard.
For ease of industrialized production, common mode is by the way of culture is expanded, and the process of spreading cultivation in the present invention can
Carry out in the following way：
First, shake-flask seed culture：Strain uses (Sphingomonas sp) CGMCCNo.1561, and slant strains are through sterile
Operation be inoculated into sterilizing seed culture medium (culture medium composition be：Glucose 1%-1.5%；Peptone 0.8%-1.0%；Ferment
Female cream 0.5%-0.8%；Surplus is water；PH value=6.5-7.5) in shaking flask, shaking table shaken cultivation 20-24 hours, shaking speed
200 revs/min, cultivation temperature is 34-36 DEG C；
2nd, first order seed culture
Primary-seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1%-1.5%；Peptone 0.6%-0.8%；Yeast extract 0.4%-0.7%；Surplus is water, pH value=
6.5-7.5。
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 20-24 hours.
3rd, secondary seed culture
Secondary seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1%-1.5%；Peptone 0.3%-0.5%；Yeast extract 0.3%-0.5%；Beef extract 0.1%-
0.15%；Surplus is water；PH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours.
Applicant verifies the stabilization using the lotion welan gum prepared by the method disclosed in the present invention with the following method
Property and solid content.
Stability, the evaluation method of solid content
First, stability
(1) test apparatus
50ml colorimetric cylinders
(2) test procedure
Sample to be tested is loaded into colorimetric cylinder to graduation mark, 24h is stood, visually observes solution without layering.
2nd, solid content
(1) test apparatus
1st, drying box
2nd, electronic balance (being accurate to 0.001g)
(2) operating procedure
The filter paper of cleaning is taken to be dried to constant weight in 95 DEG C of -105 DEG C of drying boxes, then by sample to be tested 10.000g 90%-
95% ethanol extraction is complete, is placed in 95 DEG C of -105 DEG C of baking ovens and dries to constant weight after being filtered with filter paper, weighs and calculate.
X=(M1-M2)/(M3-M2) × 100%
In formula：M1：The quality (g) of sample after filter paper and drying；
M2：Filter paper quality (g)；
M3：The quality (g) of filter paper and sample.
Substantive distinguishing features acquired by the present invention and significantly technological progress are：
1st, method of the invention is simple, and raw material sources are easy, it is easy to accomplish industrialized production.
2nd, prepared lotion welan gum stability is good, is easy to industrial transport and use, using convenience, applied to petroleum drilling
Recovery ratio is not less than existing solid welan gum in well, non-dusting during use, while safety in utilization is ensured, has
Beneficial to environmental protection and reduce loss.
Embodiment
The invention will be further described with reference to embodiments, but not as a limitation of the invention, guarantor of the invention
Shield scope is defined by the content that claim is recorded, and any equivalent technical elements made according to specification are replaced, and are not taken off
From protection scope of the present invention.
Embodiment 1
The preparation method of lotion welan gum, including by Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561
It is inoculated in containing carbon source, nitrogen source, inorganic salts and water without being fermented in bacteria fermentation culture medium；It is further comprising the steps of：
A, extract
To being extracted in the zymotic fluid after fermentation ends, extractant makes to mix from one kind in ethanol or ethanol solution
It is 75% to close the concentration of alcohol in liquid；
B, separation of solid and liquid
Separation of solid and liquid is carried out to mixed material and obtains solid material；
C, lotion welan gum is produced
Solid material adds water to be configured to lotion welan gum through colloid mill.
Extraction time in described step A is 40 minutes.
Maintain 10 minutes after zymotic fluid after fermentation ends is warming up into 90 DEG C, extracted after being cooled to 60 DEG C.
Solid material water content in described step B, C is 35%.
Solid content in described step C lotion welan gum is 3.46%.
The nitrogen source of described fermentation medium selects ammonium dihydrogen phosphate.
Described fermentation medium is made up of the component of weight/mass percentage composition：
Glucose 6.5%；Diammonium hydrogen phosphate 0.5%；Dipotassium hydrogen phosphate 0.3%；Surplus is water；PH=6.5-7.5.
Described fermentation condition is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours；It is fermentation broth viscosity not regrowth to put tank standard.
The process of spreading cultivation in the present embodiment can be carried out in the following way：
First, shake-flask seed culture：Strain uses (Sphingomonas sp) CGMCCNo.1561, and slant strains are through sterile
Operation be inoculated into sterilizing seed culture medium (culture medium composition be：Glucose 1.5%；Peptone 1.0%；Yeast extract 0.8%；
Surplus is water；PH value=6.5-7.5) in shaking flask, shaking table shaken cultivation 20-24 hours, 200 revs/min of shaking speed, culture temperature
Spend for 34-36 DEG C；
2nd, first order seed culture
Primary-seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.5%；Peptone 0.8%；Yeast extract 0.7%；Surplus is water, pH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 20-24 hours.
3rd, secondary seed culture
Secondary seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.5%；Peptone 0.5%；Yeast extract 0.5%；Beef extract 0.15%；Surplus is water；PH value=6.5-
7.5。
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours.
Embodiment 2
The preparation method of lotion welan gum, including by Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561
It is inoculated in containing carbon source, nitrogen source, inorganic salts and water without being fermented in bacteria fermentation culture medium；It is further comprising the steps of：
A, extract
To being extracted in the zymotic fluid after fermentation ends, extractant makes to mix from one kind in ethanol or ethanol solution
It is 70% to close the concentration of alcohol in liquid；
B, separation of solid and liquid
Separation of solid and liquid is carried out to mixed material and obtains solid material；
C, lotion welan gum is produced
Solid material adds water to be configured to lotion welan gum through colloid mill.
Extraction time in described step A is 30 minutes.
Maintain 5 minutes after zymotic fluid after fermentation ends is warming up into 80 DEG C, extracted after being cooled to 50 DEG C.
Solid material water content in described step B, C is 30%.
Solid content in described step C lotion welan gum is 3.08%.
The nitrogen source of described fermentation medium selects ammonium dihydrogen phosphate.
Described fermentation medium is made up of the component of weight/mass percentage composition：
Glucose 6%；Diammonium hydrogen phosphate 0.3%；Dipotassium hydrogen phosphate 0.2%；Surplus is water；PH=6.5-7.5.
Described fermentation condition is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours；It is fermentation broth viscosity not regrowth to put tank standard.
The process of spreading cultivation in the present embodiment can be carried out in the following way：
First, shake-flask seed culture：Strain uses (Sphingomonas sp) CGMCCNo.1561, and slant strains are through sterile
Operation be inoculated into sterilizing seed culture medium (culture medium composition be：Glucose 1%；Peptone 0.8%；Yeast extract 0.5%；It is remaining
Measure as water；PH value=6.5-7.5) in shaking flask, shaking table shaken cultivation 20-24 hours, 200 revs/min of shaking speed, cultivation temperature
For 34-36 DEG C；
2nd, first order seed culture
Primary-seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1%；Peptone 0.6%；Yeast extract 0.4%；Surplus is water, pH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 20-24 hours.
3rd, secondary seed culture
Secondary seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1%；Peptone 0.3%；Yeast extract 0.3%；Beef extract 0.1%；Surplus is water；PH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours.
Embodiment 3
The preparation method of lotion welan gum, including by Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561
It is inoculated in containing carbon source, nitrogen source, inorganic salts and water without being fermented in bacteria fermentation culture medium；It is further comprising the steps of：
A, extract
To being extracted in the zymotic fluid after fermentation ends, extractant makes to mix from one kind in ethanol or ethanol solution
It is 72% to close the concentration of alcohol in liquid；
B, separation of solid and liquid
Separation of solid and liquid is carried out to mixed material and obtains solid material；
C, lotion welan gum is produced
Solid material adds water to be configured to lotion welan gum through colloid mill.
Extraction time in described step A is 35 minutes.
Maintain 8 minutes after zymotic fluid after fermentation ends is warming up into 85 DEG C, extracted after being cooled to 55 DEG C.
Solid material water content in described step B, C is 32%.
Solid content in described step C lotion welan gum is 3.35%.
The nitrogen source of described fermentation medium selects ammonium dihydrogen phosphate.
Described fermentation medium is made up of the component of weight/mass percentage composition：
Glucose 6.5%；Diammonium hydrogen phosphate 0.4%；Dipotassium hydrogen phosphate 0.25%；Surplus is water；PH=6.5-7.5.
Described fermentation condition is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours；It is fermentation broth viscosity not regrowth to put tank standard.
The process of spreading cultivation in the present embodiment can be carried out in the following way：
First, shake-flask seed culture：Strain uses (Sphingomonas sp) CGMCCNo.1561, and slant strains are through sterile
Operation be inoculated into sterilizing seed culture medium (culture medium composition be：Glucose 1.2%；Peptone 0.9%；Yeast extract 0.6%；
Surplus is water；PH value=6.5-7.5) in shaking flask, shaking table shaken cultivation 20-24 hours, 200 revs/min of shaking speed, culture temperature
Spend for 34-36 DEG C；
2nd, first order seed culture
Primary-seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.2%；Peptone 0.7%；Yeast extract 0.5%；Surplus is water, pH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 20-24 hours.
3rd, secondary seed culture
Secondary seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.2%；Peptone 0.4%；Yeast extract 0.4%；Beef extract 0.12%；Surplus is water；PH value=6.5-
7.5。
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours.
Embodiment 4
The preparation method of lotion welan gum, including by Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561
It is inoculated in containing carbon source, nitrogen source, inorganic salts and water without being fermented in bacteria fermentation culture medium；It is further comprising the steps of：
A, extract
To being extracted in the zymotic fluid after fermentation ends, extractant makes to mix from one kind in ethanol or ethanol solution
It is 72% to close the concentration of alcohol in liquid；
B, separation of solid and liquid
Separation of solid and liquid is carried out to mixed material and obtains solid material；
C, lotion welan gum is produced
Solid material adds water to be configured to lotion welan gum through colloid mill.
Extraction time in described step A is 38 minutes.
Maintain 9 minutes after zymotic fluid after fermentation ends is warming up into 88 DEG C, extracted after being cooled to 58 DEG C.
Solid material water content in described step B, C is 32%.
Solid content in described step C lotion welan gum is 3.43%.
The nitrogen source of described fermentation medium selects ammonium dihydrogen phosphate.
Described fermentation medium is made up of the component of weight/mass percentage composition：
Glucose 6.5%；Diammonium hydrogen phosphate 0.4%；Dipotassium hydrogen phosphate 0.25%；Surplus is water；PH=6.5-7.5.
Described fermentation condition is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours；It is fermentation broth viscosity not regrowth to put tank standard.
The process of spreading cultivation in the present embodiment can be carried out in the following way：
First, shake-flask seed culture：Strain uses (Sphingomonas sp) CGMCCNo.1561, and slant strains are through sterile
Operation be inoculated into sterilizing seed culture medium (culture medium composition be：Glucose 1%-1.5%；Peptone 0.8%-1.0%；Ferment
Female cream 0.5%-0.8%；Surplus is water；PH value=6.5-7.5) in shaking flask, shaking table shaken cultivation 20-24 hours, shaking speed
200 revs/min, cultivation temperature is 34-36 DEG C；
2nd, first order seed culture
Primary-seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.5%；Peptone 0.65%；Yeast extract 0.5%；Surplus is water, pH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 20-24 hours.
3rd, secondary seed culture
Secondary seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.5%；Peptone 0.3%；Yeast extract 0.5%；Beef extract 0.15%；Surplus is water；PH value=6.5-
7.5。
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours.
Embodiment 5
The preparation method of lotion welan gum, including by Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561
It is inoculated in containing carbon source, nitrogen source, inorganic salts and water without being fermented in bacteria fermentation culture medium；It is further comprising the steps of：
A, extract
To being extracted in the zymotic fluid after fermentation ends, extractant makes to mix from one kind in ethanol or ethanol solution
The concentration of alcohol closed in liquid is 70%-75%；
B, separation of solid and liquid
Separation of solid and liquid is carried out to mixed material and obtains solid material；
C, lotion welan gum is produced
Solid material adds water to be configured to lotion welan gum through colloid mill.
Extraction time in described step A is 40 minutes.
Maintain 10 minutes after zymotic fluid after fermentation ends is warming up into 80 DEG C, extracted after being cooled to 60 DEG C.
Solid material water content in described step B, C is 30%.
Solid content in described step C lotion welan gum is 3.12%.
The nitrogen source of described fermentation medium selects ammonium dihydrogen phosphate.
Described fermentation medium is made up of the component of weight/mass percentage composition：
Glucose 6%；Diammonium hydrogen phosphate 0.5%；Dipotassium hydrogen phosphate 0.28%；Surplus is water；PH=6.5-7.5.
Described fermentation condition is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours；It is fermentation broth viscosity not regrowth to put tank standard.
The process of spreading cultivation in the present embodiment can be carried out in the following way：
First, shake-flask seed culture：Strain uses (Sphingomonas sp) CGMCCNo.1561, and slant strains are through sterile
Operation be inoculated into sterilizing seed culture medium (culture medium composition be：Glucose 1%；Peptone 0.9；Yeast extract 0.6；Surplus is
Water；PH value=6.5-7.5) in shaking flask, shaking table shaken cultivation 20-24 hours, 200 revs/min of shaking speed, cultivation temperature is
34-36℃；
2nd, first order seed culture
Primary-seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.5%；Peptone 0.7%；Yeast extract 0.6%；Surplus is water, pH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 20-24 hours.
3rd, secondary seed culture
Secondary seed medium is formed using the component of following weight/mass percentage composition：
Glucose 1.5%；Peptone 0.4；Yeast extract 0.4；Beef extract 0.1%；Surplus is water；PH value=6.5-7.5.
Condition of culture is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours.
Applicant is carried out using experiment to the stability of lotion welan gum and solid content that are prepared in above-described embodiment 1-5
Measure is as a result as follows：
| Stability | Solid content | |
| Embodiment 1 | Solution is without layering | 3.46% |
| Embodiment 2 | Solution is without layering | 3.08% |
| Embodiment 3 | Solution is without layering | 3.35% |
| Embodiment 4 | Solution is without layering | 3.43% |
| Embodiment 5 | Solution is without layering | 3.12% |
Claims (5)
1. the preparation method of lotion welan gum, including Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is connect
Kind is fermented in containing carbon source, nitrogen source, inorganic salts and water without bacteria fermentation culture medium；Characterized by further comprising following step
Suddenly：
A, extract
To being extracted in the zymotic fluid after fermentation ends, extractant makes mixed liquor from one kind in ethanol or ethanol solution
In concentration of alcohol be 70%-75%；
B, separation of solid and liquid
Separation of solid and liquid is carried out to mixed material and obtains solid material；
C, lotion welan gum is produced
Solid material adds water to be configured to lotion welan gum through colloid mill；
Solid material water content in described step B, C is 30%-35%, and the solid content in step C lotion welan gum contains
Measure as 3%-3.5%；
Described fermentation medium is made up of the component of weight/mass percentage composition：Glucose 6%-6.5%；Diammonium hydrogen phosphate 0.3%-
0.5%；Dipotassium hydrogen phosphate 0.2%-0.3%；Surplus is water；PH=6.5-7.5.
2. the preparation method of lotion welan gum according to claim 1, it is characterised in that during extraction in described step A
Between be not less than 30 minutes.
3. the preparation method of lotion welan gum according to claim 1, it is characterised in that during extraction in described step A
Between be 30-40 minutes.
4. the preparation method of the lotion welan gum according to any one of claim 1-3, it is characterised in that described step A
The middle zymotic fluid by after fermentation ends maintains 5-10 minutes after being warming up to 80-90 DEG C, is extracted after being cooled to 50-60 DEG C.
5. the preparation method of lotion welan gum according to claim 1, it is characterised in that described fermentation condition is：
Inoculum concentration is 10%；Cultivation temperature is 34 DEG C -36 DEG C；Ventilation is 0.3vvm-0.5vvm；Tank pressure is 0.08MPa-
0.10MPa；Kind age is 10-15 hours；It is fermentation broth viscosity not regrowth to put tank standard.
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| CN110079569B (en) * | 2019-04-29 | 2023-02-28 | 河北鑫合生物化工有限公司 | Method for producing microbial polysaccharide-sanzan gum by taking sphingosine monad as strain |
| CN110042135B (en) * | 2019-05-28 | 2022-11-18 | 河北鑫合生物化工有限公司 | Improved method for extracting welan gum from welan gum fermentation liquor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844155A (en) * | 2006-03-24 | 2006-10-11 | 张禹 | Method for preparing a welan gum |
| CN102618468A (en) * | 2012-03-28 | 2012-08-01 | 南京工业大学 | Temperature-resistant alcaligenes and application thereof in welan gum production |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844155A (en) * | 2006-03-24 | 2006-10-11 | 张禹 | Method for preparing a welan gum |
| CN102618468A (en) * | 2012-03-28 | 2012-08-01 | 南京工业大学 | Temperature-resistant alcaligenes and application thereof in welan gum production |
Non-Patent Citations (2)
| Title |
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| Welan gum:microbial production,characterization,and applications;Varinder Kaur et al;《International Journal of Biological Macromolecules》;20140206;454-461 * |
| 细菌胞外多糖提取分离技术研究进展;王桂兰等;《食品与药品》;20101231;217-219 * |
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