CN104404101A - Preparation method of paste welan gum - Google Patents
Preparation method of paste welan gum Download PDFInfo
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- CN104404101A CN104404101A CN201410631253.9A CN201410631253A CN104404101A CN 104404101 A CN104404101 A CN 104404101A CN 201410631253 A CN201410631253 A CN 201410631253A CN 104404101 A CN104404101 A CN 104404101A
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Abstract
The invention belongs to the field of preparation of microbiological polysaccharide solution, and especially relates to a preparation method of a paste welan gum. The preparation method comprises the following steps: inoculating sphingomonas paucimobilis (CGMCC No.1561) to a sterile fermentation culture medium, which contains a carbon source, a nitrogen source, inorganic salts, and water; then carrying out extraction and solid-liquid separation, and finally manufacturing the paste welan gum. In the prior art, the welan gum powder cannot be directly applied to oil-based slurry in petroleum drilling wells and the operation safety of welan gum powder is bad. The provided paste welan gum solves the problems mentioned above. The preparation method is simple. The paste welan gum is suitable for industrial production, transportation and preservation. The stability of the paste welan gum is good. The paste welan gum can be rapidly and evenly mixed with oil-based slurry. Moreover, no dust is generated during the using process, the environment is protected, and the material consumption is reduced.
Description
Technical field
The invention belongs to the preparation of microbial polysaccharide, particularly a kind of preparation method of lotion welan gum.
Background technology
Welan gum (WELAN GUM) is a kind of technical grade microbial polysaccharide, and it contains the acetyl ester group of neutral sugar, D-Glucose, D=glucuronic acid, L-rhamnosyl and L=seminose and glycosidic linkage conjunction.JanssonPE, LindbergB and Widmalm G (1985) is at " carbohydrate compound research " (139, the structure of this polysaccharide is described 217-223), the patent No. is the preparation method disclosing a kind of welan gum in the patent of invention of 200610012511.0, current welan gum is pressed powder and has good heat resistance, its aqueous solution has good viscosity and rheological property, the calcium ion of energy enduring high-concentration, cement additive is can be used as cement industry, increase denseness and the rheological characteristics of ore in sand form, improve the stability of concrete self-compacting, increase the resistance to compression bleeding of slurry concrete.Can be used for petroleum drilling and mining industry, for oil mud provides thickening, suspension and rheological characteristics, also can be used for the industry such as fine chemistry industry, daily use chemicals.When welan gum in the wild or offshore operation uses, powder easily flies upward, and loss amount is large and descend slowly and lightly and after work top meets water, form solution make worktable become sliding, poor stability when operator operate; And Powdered welan gum dissolves when directly adding oil-base mud in petroleum drilling in use and not exclusively, easily lumps, affect service efficiency; Powdered welan gum also also exists the problems such as use procedure is comparatively loaded down with trivial details, not easy to operate in addition.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of lotion welan gum, prepared welan gum is surely fast without dust, dissolution rate.
Overall technology design of the present invention is:
The preparation method of lotion welan gum, comprises and Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is inoculated in fermenting without in bacteria fermentation culture medium containing carbon source, nitrogenous source, inorganic salt and water; Further comprising the steps of:
A, extraction
Extract in the fermented liquid after fermentation ends, the one in ethanol or ethanolic soln selected by extraction agent, makes the alcohol concn in mixed solution be 70%-75%;
B, solid-liquid separation
Solid-liquid separation is carried out to mixed material and obtains solid materials;
C, produce lotion welan gum
Solid materials adds water and is mixed with lotion welan gum through colloidal mill.
Extraction agent selects ethanol or ethanolic soln, and Main Function plays anticorrosion and environmental-protection function, and the material after simultaneously ensureing extraction does not stick together phenomenon, be convenient to add water produce paste welan gum time easy handling.
Preferred technical scheme is, the extraction time in described steps A is for being not less than 30 minutes.
More preferred technical scheme is, the extraction time in described steps A is 30-40 minute.
For reaching the preservative effect of the rear material of extraction further, preferred technical scheme is, maintains 5-10 minute, extract after being cooled to 50-60 DEG C after the fermented liquid after fermentation ends being warmed up to 80-90 DEG C in described steps A.
For ease of the preparation of lotion welan gum, preferred technical scheme is, the solid materials water content in described step B, C is 30%-35%.
Solid content in the lotion welan gum of described step C is 3%-3.5%.
For reducing the particulate material in fermention medium, the impurity remained in solid materials after ensureing fermentation, extraction is few, and preferred technical scheme is, the nitrogenous source of described fermention medium selects Secondary ammonium phosphate.
For meeting the few requirement of the rear material impurity of fermentation further, ensure the needs normally carrying out and improve product yield of fermentation simultaneously.Preferred technical scheme is, described fermention medium is made up of the component of mass percentage:
Glucose 6%-6.5%; Secondary ammonium phosphate 0.3%-0.5%; Dipotassium hydrogen phosphate 0.2%-0.3%; Surplus is water; PH=6.5-7.5.
Described fermentation condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour; Putting tank standard is fermentation broth viscosity not regrowth.
For ease of suitability for industrialized production, common mode is the mode adopting enlarged culturing, and the process of spreading cultivation in the present invention can be carried out in the following way:
One, shake-flask seed is cultivated: bacterial classification adopts (Sphingomonas sp) CGMCCNo.1561, and slant strains is inoculated into the seed culture medium of sterilizing through aseptic technique, and (substratum consists of: glucose 1%-1.5%; Peptone 0.8%-1.0%; Yeast extract paste 0.5%-0.8%; Surplus is water; PH value=6.5-7.5) in shaking flask, shaking table shaking culture 20-24 hour, shaking speed 200 revs/min, culture temperature is 34-36 DEG C;
Two, first order seed is cultivated
Primary-seed medium adopts the component of following mass percentage to form:
Glucose 1%-1.5%; Peptone 0.6%-0.8%; Yeast extract paste 0.4%-0.7%; Surplus is water, pH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 20-24 hour.
Three, secondary seed is cultivated
Secondary seed medium adopts the component of following mass percentage to form:
Glucose 1%-1.5%; Peptone 0.3%-0.5%; Yeast extract paste 0.3%-0.5%; Extractum carnis 0.1%-0.15%; Surplus is water; PH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour.
Applicant adopts following method validation to utilize stability and the solid content of the lotion welan gum disclosed in the present invention prepared by method.
The evaluation method of stability, solid content
One, stability
(1) test apparatus
50ml colorimetric cylinder
(2) testing sequence
Sample to be tested is loaded colorimetric cylinder to scale marks, leave standstill 24h, visual observation solution is without layering.
Two, solid content
(1) test apparatus
1, loft drier
2, electronic balance (being accurate to 0.001g)
(2) operation steps
Get clean filter paper to dry to constant weight in 95 DEG C of-105 DEG C of loft drier, then that the alcohol extraction of sample to be tested 10.000g 90%-95% is complete, be placed in 95 DEG C of-105 DEG C of baking ovens with filter paper filtering and dry to constant weight, weigh and calculate.
X=(M
1-M
2)/(M
3-M
2)×100%
In formula: M
1: the quality (g) of sample after filter paper and drying;
M
2: filter paper quality (g);
M
3: the quality (g) of filter paper and sample.
Substantive distinguishing features acquired by the present invention and significantly technical progress are:
1, method of the present invention is simple, and raw material sources are easy, are easy to realize suitability for industrialized production.
2, prepared lotion welan gum good stability, is convenient to industrial transport and use, and application is convenient, be applied to recovery ratio in petroleum drilling and be not less than existing solid welan gum, without dust from flying in use procedure, while guarantee safety in utilization, be conducive to environmental protection and decrease loss.
Embodiment
Below in conjunction with embodiment, the invention will be further described; but it is not as a limitation of the invention; the content that protection scope of the present invention is recorded with claim is as the criterion, and any equivalent technical elements made according to specification sheets is replaced, and does not all depart from protection scope of the present invention.
Embodiment 1
The preparation method of lotion welan gum, comprises and Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is inoculated in fermenting without in bacteria fermentation culture medium containing carbon source, nitrogenous source, inorganic salt and water; Further comprising the steps of:
A, extraction
Extract in the fermented liquid after fermentation ends, the one in ethanol or ethanolic soln selected by extraction agent, makes the alcohol concn in mixed solution be 75%;
B, solid-liquid separation
Solid-liquid separation is carried out to mixed material and obtains solid materials;
C, produce lotion welan gum
Solid materials adds water and is mixed with lotion welan gum through colloidal mill.
Extraction time in described steps A is 40 minutes.
Maintain 10 minutes after fermented liquid after fermentation ends is warmed up to 90 DEG C, extract after being cooled to 60 DEG C.
Solid materials water content in described step B, C is 35%.
Solid content in the lotion welan gum of described step C is 3.46%.
The nitrogenous source of described fermention medium selects primary ammonium phosphate.
Described fermention medium is made up of the component of mass percentage:
Glucose 6.5%; Secondary ammonium phosphate 0.5%; Dipotassium hydrogen phosphate 0.3%; Surplus is water; PH=6.5-7.5.
Described fermentation condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour; Putting tank standard is fermentation broth viscosity not regrowth.
The process of spreading cultivation in the present embodiment can be carried out in the following way:
One, shake-flask seed is cultivated: bacterial classification adopts (Sphingomonas sp) CGMCCNo.1561, and slant strains is inoculated into the seed culture medium of sterilizing through aseptic technique, and (substratum consists of: glucose 1.5%; Peptone 1.0%; Yeast extract paste 0.8%; Surplus is water; PH value=6.5-7.5) in shaking flask, shaking table shaking culture 20-24 hour, shaking speed 200 revs/min, culture temperature is 34-36 DEG C;
Two, first order seed is cultivated
Primary-seed medium adopts the component of following mass percentage to form:
Glucose 1.5%; Peptone 0.8%; Yeast extract paste 0.7%; Surplus is water, pH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 20-24 hour.
Three, secondary seed is cultivated
Secondary seed medium adopts the component of following mass percentage to form:
Glucose 1.5%; Peptone 0.5%; Yeast extract paste 0.5%; Extractum carnis 0.15%; Surplus is water; PH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour.
Embodiment 2
The preparation method of lotion welan gum, comprises and Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is inoculated in fermenting without in bacteria fermentation culture medium containing carbon source, nitrogenous source, inorganic salt and water; Further comprising the steps of:
A, extraction
Extract in the fermented liquid after fermentation ends, the one in ethanol or ethanolic soln selected by extraction agent, makes the alcohol concn in mixed solution be 70%;
B, solid-liquid separation
Solid-liquid separation is carried out to mixed material and obtains solid materials;
C, produce lotion welan gum
Solid materials adds water and is mixed with lotion welan gum through colloidal mill.
Extraction time in described steps A is 30 minutes.
Maintain 5 minutes after fermented liquid after fermentation ends is warmed up to 80 DEG C, extract after being cooled to 50 DEG C.
Solid materials water content in described step B, C is 30%.
Solid content in the lotion welan gum of described step C is 3.08%.
The nitrogenous source of described fermention medium selects primary ammonium phosphate.
Described fermention medium is made up of the component of mass percentage:
Glucose 6%; Secondary ammonium phosphate 0.3%; Dipotassium hydrogen phosphate 0.2%; Surplus is water; PH=6.5-7.5.
Described fermentation condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour; Putting tank standard is fermentation broth viscosity not regrowth.
The process of spreading cultivation in the present embodiment can be carried out in the following way:
One, shake-flask seed is cultivated: bacterial classification adopts (Sphingomonas sp) CGMCCNo.1561, and slant strains is inoculated into the seed culture medium of sterilizing through aseptic technique, and (substratum consists of: glucose 1%; Peptone 0.8%; Yeast extract paste 0.5%; Surplus is water; PH value=6.5-7.5) in shaking flask, shaking table shaking culture 20-24 hour, shaking speed 200 revs/min, culture temperature is 34-36 DEG C;
Two, first order seed is cultivated
Primary-seed medium adopts the component of following mass percentage to form:
Glucose 1%; Peptone 0.6%; Yeast extract paste 0.4%; Surplus is water, pH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 20-24 hour.
Three, secondary seed is cultivated
Secondary seed medium adopts the component of following mass percentage to form:
Glucose 1%; Peptone 0.3%; Yeast extract paste 0.3%; Extractum carnis 0.1%; Surplus is water; PH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour.
Embodiment 3
The preparation method of lotion welan gum, comprises and Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is inoculated in fermenting without in bacteria fermentation culture medium containing carbon source, nitrogenous source, inorganic salt and water; Further comprising the steps of:
A, extraction
Extract in the fermented liquid after fermentation ends, the one in ethanol or ethanolic soln selected by extraction agent, makes the alcohol concn in mixed solution be 72%;
B, solid-liquid separation
Solid-liquid separation is carried out to mixed material and obtains solid materials;
C, produce lotion welan gum
Solid materials adds water and is mixed with lotion welan gum through colloidal mill.
Extraction time in described steps A is 35 minutes.
Maintain 8 minutes after fermented liquid after fermentation ends is warmed up to 85 DEG C, extract after being cooled to 55 DEG C.
Solid materials water content in described step B, C is 32%.
Solid content in the lotion welan gum of described step C is 3.35%.
The nitrogenous source of described fermention medium selects primary ammonium phosphate.
Described fermention medium is made up of the component of mass percentage:
Glucose 6.5%; Secondary ammonium phosphate 0.4%; Dipotassium hydrogen phosphate 0.25%; Surplus is water; PH=6.5-7.5.
Described fermentation condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour; Putting tank standard is fermentation broth viscosity not regrowth.
The process of spreading cultivation in the present embodiment can be carried out in the following way:
One, shake-flask seed is cultivated: bacterial classification adopts (Sphingomonas sp) CGMCCNo.1561, and slant strains is inoculated into the seed culture medium of sterilizing through aseptic technique, and (substratum consists of: glucose 1.2%; Peptone 0.9%; Yeast extract paste 0.6%; Surplus is water; PH value=6.5-7.5) in shaking flask, shaking table shaking culture 20-24 hour, shaking speed 200 revs/min, culture temperature is 34-36 DEG C;
Two, first order seed is cultivated
Primary-seed medium adopts the component of following mass percentage to form:
Glucose 1.2%; Peptone 0.7%; Yeast extract paste 0.5%; Surplus is water, pH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 20-24 hour.
Three, secondary seed is cultivated
Secondary seed medium adopts the component of following mass percentage to form:
Glucose 1.2%; Peptone 0.4%; Yeast extract paste 0.4%; Extractum carnis 0.12%; Surplus is water; PH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour.
Embodiment 4
The preparation method of lotion welan gum, comprises and Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is inoculated in fermenting without in bacteria fermentation culture medium containing carbon source, nitrogenous source, inorganic salt and water; Further comprising the steps of:
A, extraction
Extract in the fermented liquid after fermentation ends, the one in ethanol or ethanolic soln selected by extraction agent, makes the alcohol concn in mixed solution be 72%;
B, solid-liquid separation
Solid-liquid separation is carried out to mixed material and obtains solid materials;
C, produce lotion welan gum
Solid materials adds water and is mixed with lotion welan gum through colloidal mill.
Extraction time in described steps A is 38 minutes.
Maintain 9 minutes after fermented liquid after fermentation ends is warmed up to 88 DEG C, extract after being cooled to 58 DEG C.
Solid materials water content in described step B, C is 32%.
Solid content in the lotion welan gum of described step C is 3.43%.
The nitrogenous source of described fermention medium selects primary ammonium phosphate.
Described fermention medium is made up of the component of mass percentage:
Glucose 6.5%; Secondary ammonium phosphate 0.4%; Dipotassium hydrogen phosphate 0.25%; Surplus is water; PH=6.5-7.5.
Described fermentation condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour; Putting tank standard is fermentation broth viscosity not regrowth.
The process of spreading cultivation in the present embodiment can be carried out in the following way:
One, shake-flask seed is cultivated: bacterial classification adopts (Sphingomonas sp) CGMCCNo.1561, and slant strains is inoculated into the seed culture medium of sterilizing through aseptic technique, and (substratum consists of: glucose 1%-1.5%; Peptone 0.8%-1.0%; Yeast extract paste 0.5%-0.8%; Surplus is water; PH value=6.5-7.5) in shaking flask, shaking table shaking culture 20-24 hour, shaking speed 200 revs/min, culture temperature is 34-36 DEG C;
Two, first order seed is cultivated
Primary-seed medium adopts the component of following mass percentage to form:
Glucose 1.5%; Peptone 0.65%; Yeast extract paste 0.5%; Surplus is water, pH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 20-24 hour.
Three, secondary seed is cultivated
Secondary seed medium adopts the component of following mass percentage to form:
Glucose 1.5%; Peptone 0.3%; Yeast extract paste 0.5%; Extractum carnis 0.15%; Surplus is water; PH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour.
Embodiment 5
The preparation method of lotion welan gum, comprises and Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is inoculated in fermenting without in bacteria fermentation culture medium containing carbon source, nitrogenous source, inorganic salt and water; Further comprising the steps of:
A, extraction
Extract in the fermented liquid after fermentation ends, the one in ethanol or ethanolic soln selected by extraction agent, makes the alcohol concn in mixed solution be 70%-75%;
B, solid-liquid separation
Solid-liquid separation is carried out to mixed material and obtains solid materials;
C, produce lotion welan gum
Solid materials adds water and is mixed with lotion welan gum through colloidal mill.
Extraction time in described steps A is 40 minutes.
Maintain 10 minutes after fermented liquid after fermentation ends is warmed up to 80 DEG C, extract after being cooled to 60 DEG C.
Solid materials water content in described step B, C is 30%.
Solid content in the lotion welan gum of described step C is 3.12%.
The nitrogenous source of described fermention medium selects primary ammonium phosphate.
Described fermention medium is made up of the component of mass percentage:
Glucose 6%; Secondary ammonium phosphate 0.5%; Dipotassium hydrogen phosphate 0.28%; Surplus is water; PH=6.5-7.5.
Described fermentation condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour; Putting tank standard is fermentation broth viscosity not regrowth.
The process of spreading cultivation in the present embodiment can be carried out in the following way:
One, shake-flask seed is cultivated: bacterial classification adopts (Sphingomonas sp) CGMCCNo.1561, and slant strains is inoculated into the seed culture medium of sterilizing through aseptic technique, and (substratum consists of: glucose 1%; Peptone 0.9; Yeast extract paste 0.6; Surplus is water; PH value=6.5-7.5) in shaking flask, shaking table shaking culture 20-24 hour, shaking speed 200 revs/min, culture temperature is 34-36 DEG C;
Two, first order seed is cultivated
Primary-seed medium adopts the component of following mass percentage to form:
Glucose 1.5%; Peptone 0.7%; Yeast extract paste 0.6%; Surplus is water, pH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 20-24 hour.
Three, secondary seed is cultivated
Secondary seed medium adopts the component of following mass percentage to form:
Glucose 1.5%; Peptone 0.4; Yeast extract paste 0.4; Extractum carnis 0.1%; Surplus is water; PH value=6.5-7.5.
Culture condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour.
Applicant adopts test to measure the stability of the lotion welan gum prepared in above-described embodiment 1-5 and solid content, and result is as follows:
Stability | Solid content | |
Embodiment 1 | Solution is without layering | 3.46% |
Embodiment 2 | Solution is without layering | 3.08% |
Embodiment 3 | Solution is without layering | 3.35% |
Embodiment 4 | Solution is without layering | 3.43% |
Embodiment 5 | Solution is without layering | 3.12% |
Claims (9)
1. the preparation method of lotion welan gum, comprises and Sphingol single-cell (Sphingomonas sp) CGMCCNo.1561 is inoculated in fermenting without in bacteria fermentation culture medium containing carbon source, nitrogenous source, inorganic salt and water; Characterized by further comprising following steps:
A, extraction
Extract in the fermented liquid after fermentation ends, the one in ethanol or ethanolic soln selected by extraction agent, makes the alcohol concn in mixed solution be 70%-75%;
B, solid-liquid separation
Solid-liquid separation is carried out to mixed material and obtains solid materials;
C, produce lotion welan gum
Solid materials adds water and is mixed with lotion welan gum through colloidal mill.
2. the preparation method of lotion welan gum according to claim 1, is characterized in that extraction time in described steps A is for being not less than 30 minutes.
3. the preparation method of lotion welan gum according to claim 1, is characterized in that the extraction time in described steps A is 30-40 minute.
4. the preparation method of the lotion welan gum according to any one of claim 1-3, maintains 5-10 minute after it is characterized in that, in described steps A, the fermented liquid after fermentation ends is warmed up to 80-90 DEG C, extracts after being cooled to 50-60 DEG C.
5. the preparation method of lotion welan gum according to claim 1, is characterized in that the solid materials water content in described step B, C is 30%-35%.
6. the preparation method of lotion welan gum according to claim 1, is characterized in that the solid content in the lotion welan gum of described step C is 3%-3.5%.
7. the preparation method of lotion welan gum according to claim 1, is characterized in that the nitrogenous source of described fermention medium selects Secondary ammonium phosphate.
8. the preparation method of the lotion welan gum according to any one of claim 1 or 7, is characterized in that described fermention medium is made up of the component of mass percentage:
Glucose 6%-6.5%; Secondary ammonium phosphate 0.3%-0.5%; Dipotassium hydrogen phosphate 0.2%-0.3%; Surplus is water; PH=6.5-7.5.
9. the preparation method of lotion welan gum according to claim 1, is characterized in that described fermentation condition is:
Inoculum size is 10%; Culture temperature is 34 DEG C-36 DEG C; Ventilation is 0.3vvm-0.5vvm; Tank pressure is 0.08MPa-0.10MPa; Planting age is 10-15 hour; Putting tank standard is fermentation broth viscosity not regrowth.
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CN110079569A (en) * | 2019-04-29 | 2019-08-02 | 河北鑫合生物化工有限公司 | The method that microbial polysaccharide-three praises glue is produced by strain of Sphingol single-cell |
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CN110079569A (en) * | 2019-04-29 | 2019-08-02 | 河北鑫合生物化工有限公司 | The method that microbial polysaccharide-three praises glue is produced by strain of Sphingol single-cell |
CN110079569B (en) * | 2019-04-29 | 2023-02-28 | 河北鑫合生物化工有限公司 | Method for producing microbial polysaccharide-sanzan gum by taking sphingosine monad as strain |
CN110042135A (en) * | 2019-05-28 | 2019-07-23 | 河北鑫合生物化工有限公司 | The improved method that welan gum is extracted from welan gum fermentation liquid |
CN110042135B (en) * | 2019-05-28 | 2022-11-18 | 河北鑫合生物化工有限公司 | Improved method for extracting welan gum from welan gum fermentation liquor |
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