CN104404011B - Amidase and its encoding gene and application - Google Patents

Amidase and its encoding gene and application Download PDF

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CN104404011B
CN104404011B CN201410618993.9A CN201410618993A CN104404011B CN 104404011 B CN104404011 B CN 104404011B CN 201410618993 A CN201410618993 A CN 201410618993A CN 104404011 B CN104404011 B CN 104404011B
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杨立荣
郭法谋
吴坚平
徐刚
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Zhejiang University ZJU
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    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
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    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01004—Amidase (3.5.1.4)

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Abstract

The invention discloses a kind of amidase and its encoding gene and application, the amino acid sequence of the amidase is as shown in SEQ ID NO.2.The present invention is cloned into amidase gene from Klebsiella oxytoca KCTC 1686 (Klebsiella oxytoca KCTC 1686) genome, it have successfully been obtained after amidase gene expression with greater catalytic vigor and stereoselectivity and high expression quantity, the amidase of wide substrate spectrum, it can be used for preparing optically pure chipal compounds, particularly important medicine intermediate 2 (4 chlorphenyl) 3 methylbutanoic acids.Racemic substrate 2 (4 chlorphenyl) 3 methylbutyryl amine is catalyzed with the recombinant amidase, when substrate conversion efficiency is close to 50%, 2 (4 chlorphenyl) 3 methylbutanoic acid optical purity of gained S configurations is 98.5%.

Description

Amidase and its encoding gene and application
Technical field
The present invention relates to genetic engineering field, more particularly to a kind of amidase and its encoding gene and application.
Background technology
Amidase is that a class can be catalyzed the hydrolase that amide hydrolysis generates corresponding carboxylic acid and ammonia.The substrate spectrum of amidase It is very wide, various natural and artificial synthesized aliphatic and aromatic amides can be hydrolyzed.Some amidases generally have stricter Chemistry, regio- and stereo-selectivity, especially there is good stereoselectivity to the α substrates containing chiral centre.It is in system There are huge potentiality in terms of standby optical voidness acid amides, carboxylic acid and its corresponding derivative compound, the attention of industrial quarters is just being increasingly subject to.
Optical voidness acid amides, carboxylic acid and its derivative are important chemical products and chirality pharmaceutical intermediate compound.Due to chiral The growth of medicine and intermediate demand, the attention and industry of people is increasingly obtained using bioanalysis or enzymatic clarification chipal compounds Change application.The key issue of bioanalysis synthesizing chiral compound is to find the biology with High level of stereoselectivity selective catalysis function Catalyst.Amidase is very wide in microbial world distribution, obtains the research of numerous research works, also achieves numerous sections and be ground into Really.Wherein Publication No. US6617139B1 patent discloses a kind of amidase, and it can be with stereo selective hydrolysis alpha-amido acyl Amine and Alpha-hydroxy amides compound；Publication No. US7070963B2 patent discloses a kind of amidase, and it can be for life Produce optically pure D- amino acid；Publication No. CN100547067C patent discloses one plant of new bacterial strain-brevibacterium epidermidis ZJB-07021 (Brevibacterium epidermidis ZJB-07021) and its prepare (S) -2,2- dimethyl in microorganism Application in cyclopropane carboxamide.The bacterial strain contains the amidase of R- isomers stereoselectivities, by by (R) -2,2- diformazans Basic ring cyclopropane carboxamide is hydrolyzed to (R) -2,2- dinethyl cyclopropane carboxylic acids, when substrate conversion efficiency is 52%, resultant product (S) the ee values of -2,2- dimethyl-cyclopropane carboxamides are 99.2%.
But the amidase studied at present also has the problem of substrate spectrum is not wide enough, especially many amidases can not have The fragrant same clan's amide compound of the hydrolysis macromolecular of effect.Furthermore the catalysis with microorganism wild strain directly to carry out substrate is anti- Should, it also there are problems that the generation yielded poorly with a large amount of accessory substances of amidase so that product purity.Therefore, how to sieve The amidase that stereoselectivity is good, vigor is high and substrate spectrum is wide is chosen, and realizes the efficient heterogenous expression of the amidase, is reduced The generation of accessory substance, there is major issue to be solved as us.
The content of the invention
The invention provides a kind of stereoselectivity is good, vigor is high, substrate spectrum is wide and the amidase of efficient heterogenous expression.
The technical solution adopted by the present invention is：
A kind of amidase, amino acid sequence is as shown in SEQ ID NO.2.Due to the particularity of amino acid sequence, Ren Hehan There are the fragment or its variant of the peptide albumen of amino acid sequence shown in SEQ ID NO.2, as long as the fragment or peptide albumen of the peptide albumen Variant and aforementioned amino acid sequences homology belong to the scope of the present invention more than 90%, and with identical enzyme activity Row.Specifically, the change may include the missing of amino acid in amino acid sequence, insertion or replace.
Present invention also offers a kind of gene of the amidase described in coding.
It is preferred that, the base sequence of described gene is as shown in SEQ ID NO.1.The base sequence is from the sour Cray of production Primary bacterium KCTC 1686 (Klebsiella oxytoca KCTC 1686), by 1515 base compositions.Due to nucleotide sequence Particularity, the variant of polynucleotides shown in any SEQ ID NO.1, as long as it is homologous with more than 90% with the polynucleotides Property, belong to the row of the scope of the present invention.The variant of the polynucleotides refers to that there are one kind one or more nucleotides to change The polynucleotide sequence of change.The variant of this polynucleotides includes substitution variants, Deletion variants and insert variation.Such as equipotential Variant, it is probably substitution, missing or the insertion of a polynucleotides, but this change will not inherently change its institute The function of the protein of coding.
The invention also discloses a kind of expression cassette, recombinant vector and the transformant of the gene.The recombinant vector is to use The nucleotide sequence of the amidase gene of the present invention is connected to built-up on various carriers by conventional method, and carrier can be city Plasmid, bacteriophage, clay or viral vector for selling etc., such as pUC, pET-30a (+), pET-21a (+), pET-22b (+), pET- 28a (+), pETDuet-1, pACYCDuet-1, pCDFDuet-1 and RSFDuet-1, but it is not limited only to these described loads Body.It is preferred that, the amidase gene product that PCR is expanded carries out double digestion by restriction enzyme, then uses T4 ligases It is connected to and is carried out with identical restriction enzyme on the expression vector pET-30a (+) of double digestion, forms amidase gene Expression vector pET-30a (+)-AmiH.
Derivant IPTG concentration, inducing temperature and induction time be also influence amidase expression quantity and activity it is important because Element.Preferably, the condition of the induction amidase gene expression is：The OD of nutrient solution600When reaching 0.8~1.2 or so, plus Enter at derivant IPTG is 0.4~0.8mM, 16~22 DEG C to its concentration and induce 20 hours.
The invention also discloses application of the described amidase in catalysis amide hydrolysis.
The reaction equation of acid amides enzyme hydrolysis acid amides is as follows：
In formula (I)~(III)：
R1For methyl, ethyl, isopropyl, hydroxyl or halogen atom；
R2For phenyl, benzene hydroxyl or halogen substituted phenyl；
R3For hydrogen atom.
The present invention discloses described amidase and prepares 2- in catalyzing hydrolysis 2- (4- chlorphenyls) -3- methylbutyryls amine again Application in (4- chlorphenyls) -3 Methylbutanoic acid.
Compared with prior art, the invention has the advantages that：
(1) genome of the present invention from Klebsiella oxytoca KCTC1686 (Klebsiella oxytoca KCTC 1686) In be cloned into amidase gene, the amidase gene expression after have successfully been obtained with greater catalytic vigor and stereoselectivity acyl Amine enzyme, can be used for preparing optically pure chipal compounds, particularly important medicine intermediate 2- (4- chlorphenyls) -3- methyl Butyric acid.Racemic substrate 2- (4- chlorphenyls) -3- methylbutyryl amine is catalyzed with the recombinant amidase, when substrate conversion efficiency is close When 50%, 2- (4- chlorphenyls) -3 Methylbutanoic acid optical purity of gained S configurations is 98.5%；
(2) amidase of the present invention not only has high catalysis activity and stereoselectivity, also with very wide substrate spectrum, both Optically pure chiral amides and carboxylic acid compound can be prepared, macromolecular aromatic amides class compound can also be hydrolyzed.
Brief description of the drawings
Fig. 1 expands electrophoretogram for the PCR of amidase gene of the present invention；
M：Nucleic acid Marker, 1 and 2：The pcr amplification product of amidase gene.
Fig. 2 is recombinant plasmid pET-30a (+)-AmiH of the present invention collection of illustrative plates.
Fig. 3 is engineering strain E.coli BL21 (DE3) of the present invention/pET-30a (+)-AmiH induced expression products SDS-PAGE；
M：Low molecule amount standard protein；1：The broken cytosol of pET-30a (+) empty plasmid control；
2：Thalline breaks cytosol after genetically engineered E.coli BL21 (DE3)/pET-30a (+)-AmiH inductions；
3：Genetically engineered E.coli BL21 (DE3)/pET-30a (+)-AmiH induction thalline break born of the same parents' supernatant；
4：Genetically engineered E.coli BL21 (DE3)/pET-30a (+)-AmiH induction thalline break born of the same parents' precipitation.
Fig. 4 detects collection of illustrative plates for the efficient liquid phase of racemic substrate 2- (4- chlorphenyls) -3- methylbutyryl amine.
Fig. 5 is the efficient liquid of recombinant amidase hydrolysis of racemic substrate 2- (4- chlorphenyls) -3- methylbutyryl amine of the present invention Mutually detect collection of illustrative plates.
Embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited only to This.
Materials and methods in embodiment are as follows：
Experimental method in the present invention is conventional method unless otherwise instructed, for details, reference can be made to the volume such as J. Pehanorm Brookers Write《Molecular Cloning:A Laboratory guide》.
Restriction enzyme EcoRI, HindIII and T4 DNA ligase used in the embodiment of the present invention is purchased from TaKaRa, precious bioengineering (Dalian) Co., Ltd；Genome extracts kit, plasmid extraction kit, the examination of DNA recovery purifyings Agent box is purchased from Axygen Hangzhou Co., Ltd；E.coli DH5 α, E.coli BL21 (DE3), plasmid pET-30a (+) are purchased from Novagen companies；DNA marker, FastPfu archaeal dna polymerase, low molecule amount standard protein, the purchase of agarose electrophoresis reagent From Beijing Quanshijin Biotechnology Co., Ltd；Primer synthesizes limited by Shanghai life work biotechnology with sequence work Company completes.Above reagent application method refers to catalogue.The Klebsiella oxytoca KCTC 1686 that the present invention is used (Klebsiella oxytoca KCTC 1686) is purchased from South Korea's Type Culture Collection (Korean Collection for Type Cultures (KCTC))
Embodiment 1
First, cloned from Klebsiella oxytoca KCTC 1686 (Klebsiella oxytoca KCTC 1686) genome AmiH genes.
According to genomic dna sequence (the GenBank accession number of Klebsiella oxytoca KCTC 1686： CP003218.1) design primer AmiH-F and AmiH-R.
AmiH-F sequences：5’-CCGGAATTCATGGCTATTCAACGTCCCACTG-3’
AmiH-R sequences：5’-TTCCCAAGCTTGTTAAAACGTCCGCCAGTCAC-3’
Restriction enzyme site EcoRI, HindIII are separately added into the primer of upstream and downstream (shown in underscore).With The genomic DNAs of Klebsiella oxytoca KCTC 1686 are template, and AmiH-F and AmiH-R are that primer enters performing PCR amplification, PCR reaction systems and reaction condition are as follows：
PCR amplification system：
PCR amplification conditions：
1) pre-degeneration：95℃5min；
2) it is denatured：98℃10s；Annealing：55℃15s；Extension：72℃60s；Circulate 30 times altogether；
3) extend：72℃10min；
4) 4 DEG C of preservation 2.0h.
Pcr amplification product is detected with 0.8% agarose gel electrophoresis, product is single band, and size is 1500bp or so (as shown in Figure 1).Purifying recovery is carried out to pcr amplification product with DNA recovery purifyings kit, specific steps are with reference to the kit Specification.
2nd, the structure of expression vector and engineering bacteria
To first purify purpose fragment and pET-30a (+) plasmid after reclaiming respectively with restriction enzyme EcoRI and HindIII carries out double digestion.With DNA recovery purifyings kit digestion products are carried out with purifying afterwards to reclaim to remove in restricted The nucleotides small fragment that enzyme cutting and digestion are got off.Finally purpose fragment is carried out with pET-30a (+) plasmid with T4 DNA ligases Connection, linked system is as shown in table 1 below：
PET-30a (+)-AmiH recombinant expression plasmid linked systems of table 1
Above-mentioned each reagent is gently mixed, and is put in metal bath and connects 12h at 16 DEG C.Turned afterwards with connection product Change E.coli DH5a competent cells, apply flat board, choose single bacterium colony progress LB Liquid Cultures, the positive that the identification of PCR methods is successfully constructed Transformant.Recombinant plasmid pET-30a is extracted from E.coli DH5a positive transformants bacterial strains with Axygen plasmid extraction kits (+)-AmiH, and convert expressive host E.coli BL21 (DE3) competent cell with it.Screen and do through Kan resistance culture bases Bacterium colony PCR, verifies the recon of conversion.Genetic engineering bacterium after checking is errorless is E.coli BL21 (DE3)/pET-30a (+)-AmiH。
3rd, the expression of amidase
The genetically engineered E.coli BL21 (DE3) of structure/pET-30a (+)-AmiH is connected to 20mL containing 50 μ g/ml In Kan LB fluid nutrient mediums, 37 DEG C of concussion and cultivates are stayed overnight.Take 1mL nutrient solutions to be forwarded to 50mL and equally contain 50 μ g/ml Kan's In fresh LB fluid nutrient mediums, 37 DEG C of concussion and cultivates to OD600When reaching 0.8 or so, addition IPTG to its concentration is 0.5mM, 20 18h is induced at DEG C.The SDS-PAGE of its induced expression product, as shown in Figure 3.
The acid amides enzyme hydrolysis butyramide of embodiment 2
Take engineering bacteria E.coli BL21 (DE3) in 50mL embodiments 1/pET-30a (+)-AmiH zymotic fluids, 10000 Thalline is collected by centrifugation in rpm, and somatic cells then are resuspended with 50mM Tris-HCl (pH 7.5) buffer solution, engineering bacteria is produced E.coliBL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount.Xiang Chong Butyramide is put into suspension to its final concentration of 20mM, catalytic reaction is carried out at 35 DEG C, is reacted 2 hours.Gas-chromatography is used afterwards The content of butyramide and butyric acid in method detection reaction system.As a result find in reaction system without butyramide, be completely converted into Butyric acid.
The acid amides enzyme hydrolysis caproamide of embodiment 3
Engineering bacteria E.coli BL21 (DE3)/pET-30a (+)-AmiH zymotic fluids in 50mL embodiments 1 are taken, Thalline is collected by centrifugation in 10000rpm, and somatic cells then are resuspended with 50mM Tris-HCl (pH 7.5) buffer solution, engineering is produced Bacterium E.coli BL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount. Caproamide is put into re-suspension liquid to its final concentration of 30mM, catalytic reaction is carried out at 30 DEG C, is reacted 2 hours.Gas phase is used afterwards The content of caproamide and caproic acid in chromatography detection reaction system.As a result find to remain without caproamide in reaction system, entirely Portion is converted into caproic acid.
The acid amides enzyme hydrolysis benzamide of embodiment 4
Take the engineering bacteria E.coli BL21 (DE3) of 50mL embodiments 1/pET-30a (+)-AmiH zymotic fluids, 10000rpm Thalline is collected by centrifugation, somatic cells then are resuspended with 50mM Tris-HCl (pH 7.0) buffer solution, engineering bacteria E.coli is produced BL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount.Into re-suspension liquid Benzamide is put into its final concentration of 20mM, catalytic reaction is carried out at 37 DEG C, is reacted 2 hours.High performance liquid chromatography is used afterwards The content of benzamide and benzoic acid in method detection reaction system.As a result find to remain without benzamide in reaction system, It is completely converted into benzoic acid.High performance liquid chromatograph used is Agilent 1100, and chromatographic column is C18Post (5 μm, 4.6 × 150mm)。
The acid amides enzyme hydrolysis niacinamide of embodiment 5
Take the engineering bacteria E.coli BL21 (DE3) of 50mL embodiments 1/pET-30a (+)-AmiH zymotic fluids, 10000rpm Thalline is collected by centrifugation, somatic cells then are resuspended with 50mM Tris-HCl (pH 8.5) buffer solution, engineering bacteria E.coli is produced BL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount.Into re-suspension liquid Niacinamide is put into its final concentration of 20mM, catalytic reaction is carried out at 25 DEG C, is reacted 2 hours.High performance liquid chromatography is used afterwards Detect the content of the niacinamide and nicotinic acid in reaction system.As a result find to remain without niacinamide in reaction system, be totally converted For nicotinic acid.High performance liquid chromatograph used is Agilent 1100, and chromatographic column is C18Post (5 μm, 4.6 × 150mm).
The acid amides enzyme hydrolysis phenyl acetamide of embodiment 6
Take the engineering bacteria E.coli BL21 (DE3) of 50mL embodiments 1/pET-30a (+)-AmiH zymotic fluids, 10000rpm Thalline is collected by centrifugation, somatic cells then are resuspended with 50mM Tris-HCl (pH 8.0) buffer solution, engineering bacteria E.coli is produced BL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount.Into re-suspension liquid Phenyl acetamide is put into its final concentration of 10mM, catalytic reaction is carried out at 37 DEG C, is reacted 2 hours.High performance liquid chromatography is used afterwards The content of phenyl acetamide and phenylacetic acid in method detection reaction system.As a result find to remain without phenyl acetamide in reaction system, It is completely converted into phenylacetic acid.High performance liquid chromatograph used is Agilent 1100, and chromatographic column is C18Post (5 μm, 4.6 × 150mm)。
The phenyl-acetamides of 7 acid amides enzyme hydrolysis of embodiment 2
Take the engineering bacteria E.coli BL21 (DE3) of 50mL embodiments 1/pET-30a (+)-AmiH zymotic fluids, 10000rpm Thalline is collected by centrifugation, somatic cells then are resuspended with 50mM Tris-HCl (pH 7.5) buffer solution, engineering bacteria E.coli is produced BL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount.Into re-suspension liquid 2- phenyl-acetamides are put into its final concentration of 10mM, catalytic reaction is carried out at 35 DEG C, are reacted 2 hours.Efficient liquid phase is used afterwards Chromatography analyzes the optical purity of conversion ratio and product.Learnt by analysis, substrate conversion efficiency is close to 50%, gained S configurations 2- phenylacetic acids optical purity be 99%.High performance liquid chromatograph used is Agilent 1100, and chromatographic column is AY-RH chiral Post (5 μm, 4.6 × 150mm, CHIRALPAK).
The diformazan basic ring propionamide of 8 acid amides enzyme hydrolysis of embodiment 2,2
Take the engineering bacteria E.coli BL21 (DE3) of 50mL embodiments 1/pET-30a (+)-AmiH zymotic fluids, 10000rpm Thalline is collected by centrifugation, somatic cells then are resuspended with 50mM Tris-HCl (pH 7.5) buffer solution, engineering bacteria E.coli is produced BL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount.Into re-suspension liquid 2,2- diformazan basic ring propionamides are put into its final concentration of 10mM, catalytic reaction is carried out at 35 DEG C, are reacted 3 hours.Afterwards with height Effect liquid phase chromatogram method analyzes the optical purity of conversion ratio and product.Learnt by analysis, substrate conversion efficiency is close to 50%, gained 2, the 2- dimethyl cyclopropionates optical purity of S configurations is 99%.High performance liquid chromatograph used is Agilent 1100, chromatographic column For AY-RH chiral columns (5 μm, 4.6 × 150mm, CHIRALPAK).
The methylbutyryl amine of 9 acid amides enzyme hydrolysis 2 (4 chlorphenyl) of embodiment 3
Take the engineering bacteria E.coli BL21 (DE3) of 50mL embodiments 1/pET-30a (+)-AmiH zymotic fluids, 10000rpm Thalline is collected by centrifugation, somatic cells then are resuspended with 50mM Tris-HCl (pH 7.5) buffer solution, engineering bacteria E.coli is produced BL21 (DE3)/pET-30a (+)-AmiH resting cell suspension.It is 3g stem cells/L to control cell loading amount.Into re-suspension liquid 2- (4- chlorphenyls) -3- methylbutyryls amine is put into its final concentration of 10mM, catalytic reaction is carried out at 35 DEG C, is reacted 6 hours.It The optical purity of conversion ratio and product is analyzed with high performance liquid chromatography afterwards.Learnt by analysis, substrate conversion efficiency is approached 50%, 2- (4- chlorphenyls) -3 Methylbutanoic acid optical purity of gained S configurations is 98.5%.High performance liquid chromatograph used is Agilent 1100, chromatographic column is AY-RH chiral columns (5 μm, 4.6 × 150mm, CHIRALPAK).
Comparative example l
Shun-Ichi Masutomo (Bioscience Biotechnology and Biochemistry, 59 (4), 720-722,1995) etc. 2- (4- chlorphenyls) -3- methyl is catalyzed with pseudomonad B21C9 (pseudomonas sp.B21C9) The hydrolysis of butyramide.It was found that 2- (4- chlorphenyls) -3- methylbutyryl amine can be hydrolyzed to by the amidase that the wild mushroom contains 2- (4- chlorphenyls) -3 Methylbutanoic acid, and strict (S) type stereoselectivity is shown, but its activity is relatively low, only 0.5 ×10-4μm ol/min/mg stem cells.The engineering bacteria E.coli BL21 (DE3) of the present invention/pET-30a (+)-AmiH catalysis 2- During (4- chlorphenyls) -3- methylbutyryl amine, its enzyme activity reaches 3.5 × 10-2μm ol/min/mg stem cells.
Comparative example 2
Sang-Hyun Lee (Joumal of Microbiology and Biotechnology, 24 (7), 936-942, Etc. 2014) will be from the acyls for biting bulkholderia cepasea LG31-3 (Burkholderia multivorans LG31-3) more Amine enzyme is purified, and have studied the water of amidase-catalysed racemic 2- (4- chlorphenyls) -3- methylbutyryl amine of the purifying Solution reaction.As a result find that the amidase displays go out (S) type stereoselectivity, when substrate conversion efficiency is 39%, gained S configurations 2- (4- chlorphenyls) -3 Methylbutanoic acid optical purity is 98%.Engineering bacteria E.coli BL21 (DE3)/pET-30a of the present invention (+)-AmiH is catalyzed 2- (4- chlorphenyls) -3- methylbutyryl amine, when substrate conversion efficiency is 39%, 2- (the 4- chlorine of gained S configurations Phenyl) -3 Methylbutanoic acid optical purity be 100%；When substrate conversion efficiency is 50%, the 2- (4- chlorphenyls) of gained S configurations- 3 Methylbutanoic acid optical purity is 98.5%.

Claims (1)

1. amidase prepares (S) -2- (4- chlorphenyls) -3- methyl fourths in catalyzing hydrolysis 2- (4- chlorphenyls) -3- methylbutyryl amine Application in acid, it is characterised in that the amino acid sequence of the amidase is as shown in SEQ ID NO.2.
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CN106754882A (en) * 2016-12-29 2017-05-31 佛山科学技术学院 A kind of method of amidase PCR
CN107142227B (en) * 2017-03-31 2020-08-21 浙江工业大学 Acid-producing klebsiella and application thereof
CN111944794B (en) * 2020-08-20 2022-05-31 中国石油大学(华东) Amidase XAM and coding gene and application thereof
CN117286192B (en) * 2023-11-27 2024-02-23 欣雅利华生物技术(上海)有限公司 Use of amide synthetases in the preparation of litaxetil intermediates and/or litaxetil

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