CN104399080A - Pig bocavirus virus egg yolk antibody oral liquid and preparation method thereof - Google Patents
Pig bocavirus virus egg yolk antibody oral liquid and preparation method thereof Download PDFInfo
- Publication number
- CN104399080A CN104399080A CN201410762710.8A CN201410762710A CN104399080A CN 104399080 A CN104399080 A CN 104399080A CN 201410762710 A CN201410762710 A CN 201410762710A CN 104399080 A CN104399080 A CN 104399080A
- Authority
- CN
- China
- Prior art keywords
- yolk antibody
- oral liquid
- egg
- sad
- pig
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention discloses a pig bocavirus virus egg yolk antibody oral liquid and a preparation method thereof. The pig bocavirus virus egg yolk antibody oral liquid is prepared from the following raw materials by volume: 95-105L of a whole egg solution containing an egg yolk antibody, 0.2-0.3L of octanoic acid, 0.2-0.3L of formaldehyde, 0.01-0.02L of thiomersalate as well as 800-1000 unit/milliliter of mycillin. By adopting the pig bocavirus virus egg yolk antibody oral liquid disclosed by the invention, the loss in the production process is reduced, the immunocompetence substances are increased, the bioactivity is high, and no clinical side effect is generated, and meanwhile the adverse influence on the antibody is reduced when the oral liquid is orally taken as the egg white contains a great amount of various proteins.
Description
Technical field
The invention belongs to pig biological product technical field, relate to sick yolk antibody oral liquid of the rich card of a boar and preparation method thereof.
Background technology
The bocavirus that pig bocavirus (Bocavirus) is under the jurisdiction of Parvoviridae parvovirus subfamily belongs to, this DNA viruses is that China finds for nearest 2 years, there is frequently-occurring, the high dead clinical epidemiological features such as rate, highly infective in local, mainly be directed to piglet, after weaned piglet, easily cause the sick mixed infections such as multisystemic exhaustion syndrome, transmissible gastroenteritis, epidemic diarrhea, cause high mortality.
A kind of Mitochondrion IgG that yolk antibody IgY is, IgY is functionally equivalent to mammal IgG, but different in structure, has the space conformation of typical immunoglobulin.Yolk antibody has lot of superiority.(1) due to the gap of birds and mammal phylogenetics, birds are more suitable for the specific antibody of producing resisting mammal antigen.Yolk antibody can not excite complement system, does not react with the antibody of resisting mammal antigen, and not with the Fc receptors bind of mammal and antibacterial, this is significant in immunologic diagnosis.(2) yolk antibody has bioactive immunoglobulin as one, is storing, produces, processes, ingests and ensureing in digestion process that its stability is very crucial.Multitest shows, IgY has good stability, acidproof, alkaline-resisting, heat-resisting.Yolk antibody is more easily preserved, and deposits 6 months antagonist activity and have no significant effect under 4 DEG C of conditions under depositing 5 years or room temperature condition.
Summary of the invention
The invention provides boar bocavirus yolk antibody oral liquid and preparation method thereof, solve the pig bocavirus yolk antibody inconvenient problem with use of injection.
The object of the invention is to be realized by following form:
The sick yolk antibody oral liquid of the rich card of one boar, comprises the following raw material calculated according to volume ratio: containing the shell egg aqueous solution 95-105 of yolk antibody, sad 0.2-0.3, formaldehyde 0.2-0.3, thimerosal 0.01-0.02; Also comprise the mycillin of 800-1000 units per ml oral liquid.
Preparation process is:
1) pig bocavirus inactivated vaccine is adopted to carry out reinforced immunological to chicken: adopt three immune programme for children, every minor tick 10 days, every each vaccine consumption of chicken is 2ml, when antibody titer reaches 1:(128-256) time, carry out high-immunity egg collection;
2) 0.1-0.2% bromo geramine is used to carry out disinfection to high-immunity egg;
3) by 2) in the egg pulp of high-immunity egg carry out emulsifying, emulsification times 7-10min, then add pH be 4.2-4.3, temperature is the hcl acidifying water of 4-6 DEG C, the addition of hcl acidifying water is 5.5-6.5 times of egg pulp volume, after mixing, be cooled to 3-5 DEG C, leave standstill 18-24 hour;
4) get 3) in the supernatant, be warmed up to 25-32 DEG C, it is sad to add, stir 1-2h, leave standstill 10-12h, filter; Wherein sad volume: supernatant volume is (0.2-0.3): (95-105);
5) formaldehyde, thimerosal, mycillin is added, make the shell egg aqueous solution of yolk antibody: sad: formaldehyde: the volume ratio of thimerosal is (95-105): (0.2-0.3): (0.2-0.3): (0.01-0.02), and the content of mycillin is 800-1000 units per ml oral liquid;
6) subpackage, preserves.
Filtered by the filter membrane of 1.0mm, 0.45mm, 0.22mm successively when step 4) is filtered.
The present invention is by clarifying yolk antibody oral liquid to obtaining after the extraction of high-immunity egg shell egg, low temperature acidification, sad process.Shell egg uses in the extraction of yolk antibody by the present invention, can directly orally use clinically, containing a large amount of different kinds of proteins in Ovum Gallus domesticus album, the value of high-immunity egg can be made full use of, improve immunity and the stress ability of animal body, bioavailability is high, has the impregnable advantage of biological activity.
Detailed description of the invention
Embodiment 1:
The formula of one boar bocavirus yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 105 L of yolk antibody, sad 0.2 L, formaldehyde 0.3 L, thimerosal 0.02 L, and the mycillin of 1000 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available pig bocavirus inactivated vaccine to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:256, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.2% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 7min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.2, temperature is 4 DEG C, hcl acidifying water addition is 6 times of egg pulp volume, mixing, place in 4 DEG C of environment and staticly settle for 18 hours, get supernatant; Hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.2.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 25 DEG C, it is sad to add, and fully mixes 1h, then leaves standstill 10h; Wherein, by volume, the supernatant: sad=105:0.2;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=105:0.2:0.3:0.02; The content of mycillin in this oral liquid is 1000 units per ml;
7) subpackage, preserves.
For determining yolk antibody oral liquid placement effect duration at normal temperatures, do following test: this product be kept in 37 DEG C of incubators, preserve 24 months, 1 week, 2 weeks respectively, January, February, April, August, December, after 16 months, 20 months, 24 months, by agar diffusion (AGP) method, the yolk antibody measuring each point in time sampling product is tired change, the results are shown in Table 1.
Table 1. room temperature places the change that oral liquid different time sections is tired
result shows:yolk antibody can deposit 16 months under the preservation of room temperature.
Quality standard:
[character] this product is weak yellow liquid.
[steriling test] is undertaken by " Chinese veterinary pharmacopoeia ", asepsis growth.
[mycoplasma inspection] is undertaken by " Chinese veterinary pharmacopoeia ", grows without mycoplasma.
[exogenous virus inspection] is undertaken by " Chinese veterinary pharmacopoeia ", conforms with the regulations.
[safety verification] with the young pigs 5 of 7 ages in days, each oral 5 parts, observe 2 weeks, all should be good for work.
[efficacy test]
1) protection detects: with the young pigs 30 of 7 ages in days, wherein 10, often only oral 5 part yolk antibodies, and another 20 contrast isolated rearings.24h, get 10 rear virus liquids of young pig intramuscular injection inoculation bocavirus dilution in test group and matched group, record 96h death condition, the protective rate of test group is more than 85%, and counteracting toxic substances matched group mortality rate is more than 80%, and young pig is not any change blank group.
2) healing power measures: with the young pig 120 of 7 ages in days, make negative control for 40, and the virus liquid of 80 eye dripping inoculation bocavirus dilution, after infecting 12h, wherein 40, often only oral this product 3 parts, every day 1 time, continuous 3 days, another 40 were done positive control, isolated rearing.72h after treatment, record each group of death condition, counteracting toxic substances positive controls mortality rate is more than 85%, and treatment group survival rate is more than 90%, and healthy negative control group 40 is not only any change.
Embodiment 2:
The formula of one boar bocavirus yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 95 L of yolk antibody, sad 0.2 L, formaldehyde 0.2 L, thimerosal 0.01 L, and the mycillin of 800 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available pig bocavirus inactivated vaccine to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:128, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.1% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 10min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.3, temperature is 6 DEG C, hcl acidifying water addition is 5.5 times of egg pulp volume, mixing, place in 3 DEG C of environment and staticly settle for 24 hours, get supernatant; Hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.3.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 32 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 12h; Wherein, by volume, the supernatant: sad=95:0.2;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=95:0.2:0.2:0.01; The content of mycillin in this oral liquid is 800 units per ml;
7) subpackage, preserves.
For determining yolk antibody oral liquid placement effect duration at normal temperatures, do following test: this product be kept in 37 DEG C of incubators, preserve 24 months, 1 week, 2 weeks respectively, January, February, April, August, December, after 16 months, 20 months, 24 months, by agar diffusion (AGP) method, the yolk antibody measuring each point in time sampling product is tired change, the results are shown in Table 1.The results are shown in Table 2.
Table 2. room temperature places the change that injection different time sections is tired
result shows:yolk antibody can deposit 16 months under the preservation of room temperature.
Quality standard:
[character] this product is weak yellow liquid.
[steriling test] is undertaken by " Chinese veterinary pharmacopoeia ", asepsis growth.
[mycoplasma inspection] is undertaken by " Chinese veterinary pharmacopoeia ", grows without mycoplasma.
[exogenous virus inspection] is undertaken by " Chinese veterinary pharmacopoeia ", conforms with the regulations.
[safety verification] with 7 Japanese instar chickling 5, each oral 5 plumage parts, observe 2 weeks, all should be good for and live.
[efficacy test]
1) protection detects: with the young pigs 30 of 7 ages in days, wherein 10, often only oral 5 part yolk antibodies, and another 20 contrast isolated rearings.24h, get 10 rear virus liquids of young pig intramuscular injection inoculation bocavirus dilution in test group and matched group, record 96h death condition, the protective rate of test group is more than 90%, and counteracting toxic substances matched group mortality rate is more than 85%, and young pig is not any change blank group.
2) healing power measures: with the young pig 120 of 7 ages in days, make negative control for 40, and the virus liquid of 80 eye dripping inoculation bocavirus dilution, after infecting 12h, wherein 40, often only oral this product 4 parts, every day 1 time, continuous 3 days, another 40 were done positive control, isolated rearing.72h after treatment, record each group of death condition, counteracting toxic substances positive controls mortality rate is more than 90%, and treatment group survival rate is more than 85%, and healthy negative control group 40 is not only any change.
Embodiment 3:
The formula of one boar bocavirus yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 100 L of yolk antibody, sad 0.3 L, formaldehyde 0.3 L, thimerosal 0.02 L, and the mycillin of 1000 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available pig bocavirus inactivated vaccine to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:256, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.2% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 8min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.3, temperature is 5 DEG C, hcl acidifying water addition is 6.5 times of egg pulp volume, mixing, place in 5 DEG C of environment and staticly settle for 20 hours, get supernatant; Hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.3.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 30 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 11h; Wherein, by volume, the supernatant: sad=100:0.3;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=100:0.3:0.3:0.02; The content of mycillin in this oral liquid is 1000 units per ml;
7) subpackage, preserves.
Embodiment 4:
The formula of one boar bocavirus yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 102 L of yolk antibody, sad 0.3 L, formaldehyde 0.2 L, thimerosal 0.01 L, and the mycillin of 1000 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available pig bocavirus inactivated vaccine to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:128, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.1% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 9min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.3, temperature is 6 DEG C, hcl acidifying water addition is 5.5 times of egg pulp volume, mixing, place in 5 DEG C of environment and staticly settle for 22 hours, get supernatant; Hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.3.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 28 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 12h; Wherein, by volume, the supernatant: sad=102:0.3;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=102:0.3:0.2:0.01; The content of mycillin in this oral liquid is 1000 units per ml;
7) subpackage, preserves.
With the young pig 90 of 7 ages in days, only feed embodiment 3 product for first group 30, only feed embodiment 4 product for second group 30,3rd group 30 only as positive controls, isolated rearing, intramuscular inoculation bocavirus dilution virus liquid, when showing clinical symptoms, start treatment, one group, two groups every draught animals take embodiment product 5ml, every day 1 time, continuous 3 days, 3rd group of commercially available normal saline 2 ml, every day 1 time, continuous 3 days.72h after treatment, records each group of morbidity and treatment situation.Table 3.
Table 3 experimental conditions
Embodiment 5:
The formula of one boar bocavirus yolk antibody oral liquid comprises following raw material: containing shell egg aqueous solution 98 L of yolk antibody, sad 0.2 L, formaldehyde 0.3 L, thimerosal 0.01 L, and the mycillin of 800 units per ml oral liquids.
Its preparation method comprises the following steps:
1) adopt commercially available pig bocavirus inactivated vaccine to carry out reinforced immunological, every each vaccine consumption of chicken is 2ml, and every minor tick 10 days, carries out three immune programme for children, when antibody titer reaches 1:256, starts to collect high-immunity egg.
2) high-immunity egg will be collected arrange, 0.2% bromo geramine sterilization, for subsequent use.
3) water dilution method acidification: by 2) step high-immunity egg shell egg squeeze into carry out in Pasteur's tank body fully emulsified, emulsification times 10min, then the egg pulp after emulsifying is added the hcl acidifying water that pH is 4.2, temperature is 4 DEG C, hcl acidifying water addition is 6 times of egg pulp volume, mixing, place in 5 DEG C of environment and staticly settle for 24 hours, get supernatant; Hcl acidifying water in water, adds hydrochloric acid make its pH value reach 4.2.
4) sad process; By the supernatant of step 3), the shell egg aqueous solution namely containing yolk antibody weighs, and be warmed up to 30 DEG C, it is sad to add, and fully mixes 2h, then leaves standstill 12h; Wherein, by volume, the supernatant: sad=98:0.2;
5) step 4) gained clear liquor is used successively to the membrane filtration of 1.0mm, 0.45mm, 0.22mm, leave and take filtrate;
6) to step 5) gained filtrate adds formaldehyde, thimerosal, mycillin; By volume, containing the shell egg shell egg aqueous solution of yolk antibody: sad: formaldehyde: thimerosal=98:0.2:0.3:0.01; The content of mycillin in this oral liquid is 800 units per ml;
7) subpackage, preserves.
Claims (3)
1. the sick yolk antibody oral liquid of the rich card of a boar, is characterized in that: comprise the following raw material calculated according to volume ratio: containing the shell egg aqueous solution 95-105 of yolk antibody, sad 0.2-0.3, formaldehyde 0.2-0.3, thimerosal 0.01-0.02; Also comprise the mycillin of 800-1000 units per ml oral liquid.
2. pig wins a preparation method for the sick yolk antibody oral liquid of card as claimed in claim 1, it is characterized in that concrete steps are:
1) pig bocavirus inactivated vaccine is adopted to carry out reinforced immunological to chicken: adopt three immune programme for children, every minor tick 10 days, every each vaccine consumption of chicken is 2ml, when antibody titer reaches 1:(128-256) time, carry out high-immunity egg collection;
2) 0.1-0.2% bromo geramine is used to carry out disinfection to high-immunity egg;
3) by 2) in the egg pulp of high-immunity egg carry out emulsifying, emulsification times 7-10min, then add pH be 4.2-4.3, temperature is the hcl acidifying water of 4-6 DEG C, the addition of hcl acidifying water is 5.5-6.5 times of egg pulp volume, after mixing, be cooled to 3-5 DEG C, leave standstill 18-24 hour;
4) get 3) in the supernatant, be warmed up to 25-32 DEG C, it is sad to add, stir 1-2h, leave standstill 10-12h, filter; Wherein sad volume: supernatant volume is (0.2-0.3): (95-105);
5) formaldehyde, thimerosal, mycillin is added, make the shell egg aqueous solution of yolk antibody: sad: formaldehyde: the volume ratio of thimerosal is (95-105): (0.2-0.3): (0.2-0.3): (0.01-0.02), and the content of mycillin is 800-1000 units per ml oral liquid;
6) subpackage, preserves.
3. pig according to claim 2 wins the preparation method of the sick yolk antibody oral liquid of card, it is characterized in that: filtered by the filter membrane of 1.0mm, 0.45mm, 0.22mm successively when step 4) is filtered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410762710.8A CN104399080A (en) | 2014-12-14 | 2014-12-14 | Pig bocavirus virus egg yolk antibody oral liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410762710.8A CN104399080A (en) | 2014-12-14 | 2014-12-14 | Pig bocavirus virus egg yolk antibody oral liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104399080A true CN104399080A (en) | 2015-03-11 |
Family
ID=52636787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410762710.8A Pending CN104399080A (en) | 2014-12-14 | 2014-12-14 | Pig bocavirus virus egg yolk antibody oral liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104399080A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716476A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Cell inactivated vaccine, egg yolk antibody injection, and preparation method of cell inactivated vaccine |
-
2014
- 2014-12-14 CN CN201410762710.8A patent/CN104399080A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716476A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Cell inactivated vaccine, egg yolk antibody injection, and preparation method of cell inactivated vaccine |
Non-Patent Citations (1)
| Title |
|---|
| 张小莺 等: "《卵黄抗体技术》", 31 May 2011, 科学出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104530232B (en) | The preparation method of duck virus hepatitis refined yolk antibody | |
| CN110251671B (en) | Preparation method of goose astrovirus egg yolk antigen-antibody complex | |
| CN104922663B (en) | A kind of newcastle disease and H9 subtype avian influenza bigeminy vaccines | |
| CN105950564B (en) | The scorching virus of foreign duck liver and the method for preparing the scorching viral refined vitelline antibody of foreign duck liver using the virus | |
| CN101475639B (en) | Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof | |
| CN103820397A (en) | Muscovy duck parvovirus and application thereof | |
| CN102716485A (en) | Dual hyper-immune egg yolk antibody injection for duck virus hepatitis and duck plague and preparation method thereof | |
| CN104922665A (en) | Triple inactivated vaccine for newcastle disease, infectious bronchitis and H9 subtype avian influenza | |
| CN105031638A (en) | Trivalent inactivated vaccine against Newcastle disease, avian influenza and infectious bursal disease | |
| CN102302775B (en) | Combined inactivated vaccine of Newcastle disease and H9 subtype avian influenza and preparation method thereof | |
| CN109097340B (en) | Avian adenovirus, quadruple vaccine and preparation method thereof | |
| CN103495167A (en) | Method for preparing chicken infection bursal disease composite live vaccine | |
| CN106563125B (en) | Duck hepatitis A virus III type compound live vaccine and preparation method thereof | |
| CN103830724B (en) | Muscovy duck parvovirus inactivation vaccine and application thereof | |
| CN102861327A (en) | Cell inactivated vaccine, egg yoke antibody and injection and freeze-dried powder containing same | |
| CN102965344B (en) | Production of infectious bronchitis virus and vaccine from cell line | |
| CN103143010B (en) | Triad inactivated vaccine for newcastle disease and bird flu (H9 subtype) and escherichia coli disease and preparation method thereof | |
| CN104399077A (en) | Newcastle disease egg yolk antibody oral liquid and preparation method thereof | |
| CN104383536A (en) | Egg yolk antibody oral liquid for duck virus hepatitis and preparation method for egg yolk antibody oral liquid | |
| CN104399080A (en) | Pig bocavirus virus egg yolk antibody oral liquid and preparation method thereof | |
| CN104383538A (en) | Tetragenous egg yolk antibody oral liquid and preparation method for same | |
| CN104491861A (en) | Classical swine fever viral egg yolk antibody oral solution and preparation method thereof | |
| CN104491860A (en) | Pet viral egg yolk antibody oral solution and preparation method thereof | |
| CN104491862A (en) | Duck viral hepatitis and duck plague bigeminal egg yolk antibody oral solution and preparation method thereof | |
| CN103834620B (en) | NDV, ewcastle disease inactivated vaccine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150311 |