CN104398701A - Extraction method and application of component group of traditional Chinese medicine volatile oil extracts for treating psoriasis - Google Patents
Extraction method and application of component group of traditional Chinese medicine volatile oil extracts for treating psoriasis Download PDFInfo
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- CN104398701A CN104398701A CN201410733406.0A CN201410733406A CN104398701A CN 104398701 A CN104398701 A CN 104398701A CN 201410733406 A CN201410733406 A CN 201410733406A CN 104398701 A CN104398701 A CN 104398701A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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Abstract
The invention discloses an extraction method and application of the component group of traditional Chinese medicine volatile oil extracts for treating psoriasis. The extraction method comprises the following steps: rheum officinale, cortex moutan, radix paeoniae rubra and rhizoma acori graminei are prepared according to a certain weight ratio, smashed and then soaked with water; a volatile oil is extracted through the vapor distillation method. Through GC-MS (gas chromatography-mass spectrometer) analysis, the traditional Chinese medicine volatile oil extracts contain 70 volatile oil components including 30.8 to 36.2 percent of beta-asarone, 14.8 to 18.2 percent of paeonol, 3.8 to 7.4 percent of alpha-asarone, and 3.8 to 5.2 percent of acorone. The traditional Chinese medicine volatile oil extracts can remarkably reduce the body temperature, whole blood viscosity and plasma viscosity of blood heat and blood stasis model rats, achieves the effects of cooling blood and invigorating blood circulation, and can be used for preparing medicines for psoriasis.
Description
One, technical field
The present invention relates to technical field of Chinese medicine, specifically a kind of extracting method and application thereof for the treatment of psoriatic traditional medicine volatile oil extract component group.
Two, background technology
Psoriasis is equivalent to the category such as Chinese medicine " in vain ", " psoriasis ", " chronic eczema ", " psoriasis ", modern times, doctor thought this disease appearing in blood system mostly, pathogeny substantially can be summarized as the dry and blood deficiency of heat in blood, blood stasis, blood, and blood is dry, blood deficiency is pathological characteristic, it is heat in blood, the pathological examination that further develops of blood stasis.Therefore, psoriatic treatment is emphasized only have heat in blood and blood stasis to be run through psoriasis pathological characteristic all the time.
Chinese traditional treatment psoriasis, often based on cool the blood dissipate blood stasis medicine, reaches removing heat from blood and promoting blood circulation effect, has good curative effect.Chinese herb rhubarb clearing away heat-fire, Cortex Moutan cool the blood dissipate blood stasis, Radix Paeoniae Rubra removing heat from blood and promoting blood circulation, Rhizoma Acori Graminei are had one's ideas straightened out refreshment.Therefore, the present invention adopts steam distillation to extract this Chinese medicine composition extractive of volatile oil of preparation, with its removing heat from blood and promoting blood circulation effect of heat in blood rat's blood stasis model model evaluation, is used for the treatment of the purposes of psoriasis.
Three, summary of the invention
For the demand of clinical psoriatic, the object of this invention is to provide a kind of extracting method and application thereof for the treatment of psoriasis extractive of volatile oil components group.
The object of the invention is to be realized by following technical scheme:
1. treat a psoriasis traditional medicine volatile oil extract component group's extracting method, extraction step is as follows:
(1) raw material: Radix Et Rhizoma Rhei 1-5 part, Cortex Moutan 1-5 part, Radix Paeoniae Rubra 1-5 part, Rhizoma Acori Graminei 1-6 part;
(2) above-mentioned raw materials medicated powder is broken to 20-60 order, adds 5-20 times amount water soaking 5-12h, steam distillation extracts 4-8 hour, collects extractive of volatile oil;
(3) Analysis of Essential Oil Components: extractive of volatile oil is analyzed through GC-MS 70 kinds of volatile oil components, and wherein beta-Asarone accounts for 30.8%-36.2%, paeonol 14.8%-18.2%, α-asaricin 3.8%-7.4%, acorone 3.8%-5.2%.
2. the application of traditional medicine volatile oil extract component group in preparation treatment psoriasis.
Beneficial effect:
Traditional medicine volatile oil extract component group of the present invention, primarily of beta-Asarone, paeonol, more than 70 volatile oil component composition such as α-asaricin, acorone etc., significantly can reduce heat in blood blood stasis model rat temperature and whole blood, Plasma Viscosity, there is removing heat from blood and promoting blood circulation effect, can be used for treatment psoriasis purposes.
Four, the pharmacological testing of volatile oil component group
1. modeling and administration
Male SD rat is divided into 4 groups at random, often organize 8, be respectively blank group (normal saline group), model group (dry yeast group), positive controls (dry yeast+aspirin group), medicine group (dry yeast+extractive of volatile oil group).10mLkg is pressed by each group
-1every day gastric infusion 1 time, continuous 7d.Modeling after the 6th day administration 1h (blank group in subcutaneous injection normal saline, all the other each group in subcutaneous injection dry yeast suspension, injected dose is 10mLkg
-1), the change of anus temperature in record rat 6h, fasting 10h, within the 7th day, gastric infusion 1h rear neck artery gets blood 5ml in being added with 0.038gml
-1in the centrifuge tube of sodium citrate, with 3000rmin
-1the centrifugal 10min of rotating speed, gets upper plasma and measures plasma viscosity, separately get whole blood and measure that whole blood is high, medium and low cuts viscosity in right amount.
Statistics adopts SPSS13.0 statistical software to carry out two sample t-test and variance analysis to data, experimental data with
represent.
2. on the impact of heat in blood rat's blood stasis model body temperature
Experimental result (table 1) shows: rat obviously raises in 5h, 6h body temperature heat in blood blood stasis model group, and model group is organized comparing difference with blank and had significance (P < 0.01).Compare with model group, positive controls can reduce the body temperature P < 0.05 of heating rat at 6h); Extractive of volatile oil significantly reduces the body temperature (P < 0.05) of heating rat between 5h.
Rectal temperature test result in table 1 rat 6h (
n=8)
Note: * P ﹤ 0.05, * * P ﹤ 0.01
3. on the impact of heat in blood rat's blood stasis model whole blood viscosity
Experimental result (table 2) shows: high, medium and low more blank group of the viscosity of cutting of the whole blood of model group significantly raises (P < 0.01).Compare with model group, positive controls can significantly reduce whole blood highly to cut, in cut viscosity (P < 0.01), low shear viscosity (P < 0.05); Extractive of volatile oil significantly can reduce that whole blood is high, medium and low cuts viscosity (P < 0.01).
Table 2 rat whole blood viscosity measurements (
n=8)
Note: * P ﹤ 0.05, * * P ﹤ 0.01
3. on the impact of heat in blood rat's blood stasis model Plasma Viscosity
Experimental result (table 3): compare with blank group, model group plasma viscosity has significant difference (P < 0.01).Compare with model group, positive controls, extractive of volatile oil can reduce Plasma Viscosity (P < 0.05).
Table 3 rat plasma viscosity measurement result (
n=8)
Note: * P ﹤ 0.05, * * P ﹤ 0.01
Five, volatile oil is drawn chemical constituent GC-MS analyzes
Chromatographic column HP-5MS Phenyl Methyl Siloxane quartz capillary chromatographic column (0.25mm × 30m, 0.25 μm); Carrier gas is helium, sample size 1 μ L, flow velocity 1 μ LmL
-1, split ratio 20: 1, injector temperature 250 DEG C, detector temperature 250 DEG C; Temperature programming: initial temperature 70 DEG C, keeps 2min, with 2 DEG C/min ramp to 150 DEG C, keeps 10min, with the ramp to 180 DEG C of 2 DEG C/min, keeps 20min, with the ramp to 230 DEG C of 2 DEG C/min, keeps 5min.Mass resolution 500, ionization mode EI, electron energy 70eV, ion source temperature 200 DEG C, accelerating potential 4kV, scanning speed 1sdec
-1, sweep limits m/z20 ~ 500.
GC-MS is adopted to analyze volatile oil is drawn chemical constituent (Fig. 1), through data handling system, its internal memory spectrum storehouse is retrieved automatically, artificial spectrum elucidation, in conjunction with documents and materials, identify 70 compounds (table 3), account for the 90.1%-95.4% of total volatile oil amount, wherein beta-Asarone accounts for 32.1%-38.8%, paeonol 10.2%-18.2%, α-asaricin 3.5%-7.4%, acorone 2.2%-6.2%.
Table 3 extractive of volatile oil composition
Result shows after deliberation, the extractive of volatile oil components group prepared by the present invention, primarily of beta-Asarone, paeonol, more than 70 volatile oil component composition such as α-asaricin, acorone etc., obviously can reduce heat in blood blood stasis model rat temperature and whole blood, Plasma Viscosity, there is removing heat from blood and promoting blood circulation effect, can be used for preparing psoriasis purposes.
Six, accompanying drawing explanation
Fig. 1 extractive of volatile oil GC-MS total ion current figure.
Seven. detailed description of the invention
Embodiment 1: the extracting method for the treatment of psoriasis traditional medicine volatile oil extract component group, extraction step is as follows:
(1) crude drug: Radix Et Rhizoma Rhei 1 part, Cortex Moutan 1 part, Radix Paeoniae Rubra 1 part, Rhizoma Acori Graminei 1 part;
(2) above-mentioned raw materials medicated powder is broken to 20 orders, add 5 times amount water soaking 5h, steam distillation extracts 4 hours, collects extractive of volatile oil;
(3) Analysis of Essential Oil Components: analyze the chemical composition of extractive of volatile oil with GC-MS, have volatile oil component more than 70, wherein beta-Asarone account for 34.8%, paeonol 14.8%, α-asaricin 5.2%, acorone 3.8%.
Embodiment 2: the extracting method for the treatment of psoriasis traditional medicine volatile oil extract component group, extraction step is as follows:
(1) crude drug: Radix Et Rhizoma Rhei 3 parts, Cortex Moutan 2 parts, Radix Paeoniae Rubra 3 parts, Rhizoma Acori Graminei 4 parts;
(2) above-mentioned raw materials medicated powder is broken to 40 orders, add 10 times amount water soaking 10h, steam distillation extracts 6 hours, collects extractive of volatile oil;
(3) Analysis of Essential Oil Components: analyze the chemical composition of extractive of volatile oil with GC-MS, have volatile oil component more than 70, wherein beta-Asarone account for 30.8%, paeonol 17.8%, α-asaricin 3.8%, acorone 4.8%.
Embodiment 3: the extracting method for the treatment of psoriasis traditional medicine volatile oil extract component group, extraction step is as follows:
(1) crude drug: Radix Et Rhizoma Rhei 5 parts, Cortex Moutan 5 parts, Radix Paeoniae Rubra 5 parts, Rhizoma Acori Graminei 6 parts;
(2) above-mentioned raw materials medicated powder is broken to 60 orders, add 20 times amount water soaking 12h, steam distillation extracts 8 hours, collects extractive of volatile oil;
(3) Analysis of Essential Oil Components: analyze the chemical composition of extractive of volatile oil with GC-MS, have volatile oil component more than 70, wherein beta-Asarone account for 36.2%, paeonol 18.2%, α-asaricin 7.4%, acorone 5.2%.
Claims (2)
1. treat a psoriatic traditional medicine volatile oil extract component group's extracting method, extraction step is as follows:
(1) crude drug: Radix Et Rhizoma Rhei 1-5 part, Cortex Moutan 1-5 part, Radix Paeoniae Rubra 1-5 part, Rhizoma Acori Graminei 1-6 part;
(2) above-mentioned raw materials medicated powder is broken to 20-60 order, adds 5-20 times amount water soaking 5-12h, steam distillation extracts 4-8 hour, collects extractive of volatile oil;
(3) Analysis of Essential Oil Components: extractive of volatile oil GC-MS analyzes 70 kinds of volatile oil components, and wherein beta-Asarone accounts for 30.8%-36.2%, paeonol 14.8%-18.2%, α-asaricin 3.8%-7.4%, acorone 3.8%-5.2%.
2. the application of traditional medicine volatile oil extract component group as claimed in claim 1 in preparation treatment psoriasis.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410733406.0A CN104398701B (en) | 2014-12-04 | 2014-12-04 | The extracting method of traditional medicine volatile oil extract component group for treating psoriasis a kind of and its application |
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|---|---|---|---|
| CN201410733406.0A CN104398701B (en) | 2014-12-04 | 2014-12-04 | The extracting method of traditional medicine volatile oil extract component group for treating psoriasis a kind of and its application |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199663A (en) * | 2006-12-14 | 2008-06-18 | 黑龙江福和华星制药集团股份有限公司 | Chinese medicine for treating psoriasis and preparing method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199663A (en) * | 2006-12-14 | 2008-06-18 | 黑龙江福和华星制药集团股份有限公司 | Chinese medicine for treating psoriasis and preparing method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 吴勉华等: "研究周仲瑛"瘀热"学说的思路与方法", 《中华中医药杂志》 * |
| 周仲瑛: "论瘀热", 《南京中医药大学学报》 * |
| 李东海等: "从瘀热互结探讨银屑病的证治", 《辽宁中医药大学学报》 * |
| 许立等: "实验性淤热证大鼠模型的初步研究", 《时珍国医国药》 * |
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