CN104398552B - A kind of Rhizoma Atractylodis Macrocephalae extract and its application with regulating blood lipid action - Google Patents

Abstract

The invention discloses a kind of Rhizoma Atractylodis Macrocephalae extract and purposes with effect for reducing blood fat, the Rhizoma Atractylodis Macrocephalae extract is prepared by the following method to obtain：Method one, take bighead atractylodes rhizome root medicinal material to pulverize and sieve, be put into extractor and first extract, then separate, Rhizoma Atractylodis Macrocephalae extract is obtained after separation；Separating and keeping CO in extraction process₂Flow：55~70L/h；Method two, bighead atractylodes rhizome root medicinal material is taken, crushed, add 3~8 times of amount petroleum ethers, 0.5~3 h of ultrasound, refluxing extraction 1~4 time, every time 0.5~8.0 h；Merging filtrate is concentrated under reduced pressure into organic solvent-free residual.The Rhizoma Atractylodis Macrocephalae extract has regulating blood lipid action.It can be widely applied to the medicine or health food with effect for reducing blood fat.

Description

Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizome and Its Application

technical field

The invention belongs to the technical field of medicine, and in particular relates to an extract of Atractylodes Rhizoma Atractylodes Rhizoma Rhizoma Rhizoma Rhizome with the function of regulating blood lipid and its application.

Background technique

With the improvement of living standards and changes in diet structure and lifestyle, the prevalence of dyslipidemia in my country has shown an obvious upward trend in recent years. Dyslipidemia is one of the manifestations of metabolic syndrome, which is closely related to many diseases, such as Obesity, hypertension, coronary heart disease, stroke, etc. Therefore, the prevention and treatment of dyslipidemia is of great significance for prolonging life and improving quality of life. Clinically, hyperlipidemia can be divided into four categories according to blood lipid levels: (1) hypercholesterolemia: elevated serum cholesterol (TC) levels; (2) hypertriglyceridemia: serum triglyceride (TG) levels (3) Mixed hyperlipidemia: both serum TC and TG levels increased; (4) Low high-density lipoproteinemia: serum high-density lipoprotein (HDL-c) level decreased. Traditional Chinese medicine has the characteristics of multi-path, multi-target and multi-channel. It is based on syndrome differentiation and treatment, and is good at improving the symptoms of different symptoms when treating hyperlipidemia, and has a certain integrity and system. In addition, it also takes into account other pathological links, can significantly improve the clinical symptoms of hyperlipidemia, and improve the quality of life of patients.

Atractylodes macrocephala is the dry rhizome of Atractylodis macrocephala Koidz. Its main active ingredients are volatile oil, polysaccharide and so on. Atractylodes Rhizoma Atractylodes Rhizome is warm in nature and sweet in taste, enters the two meridians of spleen and stomach, and has the effects of invigorating the spleen and replenishing qi, drying dampness and diuresis, strengthening the surface, antiperspirant, and antifertility. Among them, atractylodes ketone and atractylodes lactone in the volatile oil have pharmacological effects such as protecting the liver, lowering blood pressure, anti-inflammation, and anti-cancer.

At present, there are few studies on the blood lipid-lowering efficacy of Atractylodes macrocephala, and the only reports are the water-extracted compound of Atractylodes macrocephala (such as Linggui Yiyi Baizhu powder, Banxia Baizhu Tianma decoction) or alcoholic extract of Atractylodes macrocephala.

The preliminary research of the present invention shows that the lipid-lowering active ingredients in the alcoholic extract of Atractylodes Rhizoma Atractylodes Rhizome are lipophilic and low-polar components, and the lipid-lowering effect of the extract obtained by the inventors using a low-polarity solvent or supercritical extraction is significantly stronger than that of the alcoholic extract. The present invention adopts _CO Supercritical extraction or low-polar solvent (petroleum ether or normal hexane, ethyl acetate, acetone and other organic solvents with low polarity) to extract the extract that is more concentrated than alcohol extracts for lipid-lowering active ingredients, In practical application, it has the advantages of small dosage and clear effect, and is more suitable for further development and application.

The present invention adopts supercritical _CO2 extraction or petroleum ether to extract the dried rhizome of Atractylodes Rhizome, and obtains the extract of Atractylodes Rhizoma Atractylodis with a content of 10% to 95% of atractylodes. The study finds that a certain dose of the extract can significantly reduce the serum concentration of hyperlipidemia rats. TC, LDL-C levels, increase HDL-C levels, down-regulate atherosclerosis index (AI), improve related blood lipid indicators, and the extraction process is short and efficient. Development prospects.

Contents of the invention

The object of the present invention is to provide an extract of Atractylodes Rhizoma Atractylodes Rhizoma Rhizoma Rhizoma Rhizoma Rhizome with the function of regulating blood lipid and its application.

The technical solution adopted by the present invention to solve the above-mentioned technical problems: a kind of Atractylodes Rhizoma Atractylodes Rhizoma Rhizoma Rhizome Extract with the function of regulating blood lipid, and the extract is obtained by _CO2 supercritical extraction, and the specific steps are as follows:

(1) Take the Atractylodes Rhizome medicinal material and grind and sieve it, and put it into an extraction tank;

(2) extraction at 20-30MPa, 40-55°C to obtain the extracted product;

(3) Separating the extracted product obtained in step 2 at 4MPa to 9MPa and 40 to 70°C to obtain the first extract and the isolated product;

(4) Re-separating the isolated product obtained in step 3 at 3 to 6 MPa and 30 to 50° C. to obtain a second extract;

(5) Collect the first extract obtained in step 3 and the second extract obtained in step 4, which is the Atractylodes Rhizoma Atractylodes extract with the effect of regulating blood lipid;

The steps 2-4 are carried out in a _CO2 medium of 55-70 L/h.

An extract of Atractylodes Rhizoma Atractylodes Rhizoma Rhizoma Rhizome with the function of regulating blood lipids, the extract is obtained through the following steps:

(1) After pulverizing the rhizome of Atractylodes Rhizome, add it into an organic solvent and ultrasonicate for 0.5-3 h, and the concentration of the rhizome of Atractylodes Rhizome is 0.125-0.33 g/L; reflux extraction 1-4 times, 0.5-8.0 h each time, Filtrate after the second reflux extraction to obtain the filtrate;

(2) Combine the filtrate in step 1, concentrate under reduced pressure, the concentration temperature is 20～55°C, the vacuum degree is-0.07～-0.09Mpa, the concentrated solution is until no organic solvent remains; the organic solvent is sherwood oil, n-hexane, Ethyl acetate or acetone.

The application of Atractylodes macrocephala extract in the preparation of medicine or food for preventing and treating hyperlipidemia.

The beneficial effect of the present invention is that the present invention selects supercritical _CO2 extraction method or low polarity solvent (such as petroleum ether) to extract the rhizome of Atractylodes Rhizoma Atractylodes Rhizome, develops its lipid-lowering effect, and expands the medicinal scope of Atractylodes Rhizome. The inventor's experiment shows: this Atractylodes Rhizome extract can reduce rat total cholesterol (TC), low-density lipoprotein (LDL-C), atherosclerotic index (AI) of model rat, raise high-density lipoprotein ( HDL-C), has the function of regulating blood lipids, and can be made into extracts mainly composed of Atractylodes macrocephala supercritical _CO2 extracts, tablets, granules, pills, capsules, powders, oral liquids, etc., mainly for the prevention and treatment of hyperlipidemia Functional medicines and health food.

detailed description

Further describe the present invention according to embodiment below, the effect of the present invention will embody more obviously.

Example 1

An extract of Atractylodes Rhizoma Atractylodes Rhizoma Rhizome with the function of regulating blood lipid, obtained through the following steps:

(1) Take the rhizome of Atractylodes Rhizome, wash it, dry it at 40-60°C, crush it, pass it through a 20-mesh sieve, and put it into a 5 L extraction tank;

(2) Extract at 20MPa and 40°C to obtain the extracted product;

(3) separating the extracted product obtained in step 2 at 4MPa, 40°C to obtain the first extract and the separated product;

(4) Re-separating the isolated product obtained in step 3 at 3 MPa and 30° C. to obtain the second extract;

(5) Collecting the first extract and the second extract, namely the Atractylodes Rhizome extract.

The steps 2-4 are carried out in 55L/h _CO2 medium.

Example 2, an extract of Rhizoma Atractylodes Rhizoma Rhizoma Rhizoma Rhizoma Rhizoma Atractylodes Rhizome that regulates blood lipid, is obtained through the following steps:

(1) Take the rhizome of Atractylodes macrocephala, wash it, dry it at 40-60°C, crush it, pass it through a 40-mesh sieve, and put it into a 5 L extraction tank

(2) extraction at 30MPa, 55°C to obtain the extracted product;

(3) separating the extracted product obtained in step 2 at 9 MPa and 70°C to obtain the first extract and the separated product;

(4) Re-separating the isolated product obtained in step 3 at 6 MPa and 50° C. to obtain the second extract;

(5) Collecting the first extract and the second extract, namely the Atractylodes Rhizome extract.

The steps 2-4 are carried out in 60L/h _CO2 medium.

Example 3, an extract of Atractylodes Rhizoma Atractylodes Rhizoma Rhizoma Atractylodes Rhizome with the function of regulating blood lipids, obtained through the following steps:

(1) Take the rhizome of Atractylodes Rhizome, wash it, dry it at 40-60°C, crush it, pass it through a 30-mesh sieve, and put it into a 5 L extraction tank;

(2) extraction at 25MPa and 50°C to obtain the extracted product;

(3) Separating the extracted product obtained in step 2 at 6 MPa and 60° C. to obtain the first extract and the separated product;

(4) Re-separating the isolated product obtained in step 3 at 5 MPa and 40° C. to obtain the second extract;

(5) Collecting the first extract and the second extract, namely the Atractylodes Rhizome extract.

The steps 2-4 are carried out in 70L/h _CO2 medium.

Example 4

Take 1Kg Atractylodes rhizome medicinal material, wash and dry, crush and sieve, add 7L petroleum ether, ultrasonic 1.5h, reflux extraction 2 times, 1.5h each time, filter after each reflux extraction; (2) Combine the filtrates and depressurize Concentrate, the concentration temperature is 20°C, the vacuum degree is -0.09Mpa, and the concentrated solution has no organic solvent residue.

Example 5

Take 1Kg Atractylodes rhizome medicinal material, wash and dry, crush and sieve, add 7L of n-hexane, ultrasonication for 1 hour, reflux extraction twice, 2 hours each time, filter after each reflux extraction; (2) combine the filtrates and concentrate under reduced pressure, The concentration temperature is 20°C, the vacuum degree is -0.09Mpa, and the concentrated solution has no organic solvent residue.

Example 6

Take 1Kg Atractylodes rhizome medicinal material, wash and dry, pulverize and sieve, add 3L ethyl acetate, ultrasonic 0.5h, reflux extraction 4 times, 0.5h each time, filter after each reflux extraction; (2) combine the filtrate to reduce Concentrate under high pressure, the concentration temperature is 55°C, the vacuum degree is -0.07Mpa, and the concentrated solution has no organic solvent residue.

Example 7

Take 1Kg Atractylodes rhizome, wash and dry, crush and sieve, add 8L acetone, ultrasonic 3h, reflux extraction once, 8h each time, filter after each reflux extraction; The temperature is 40°C, the vacuum degree is -0.08Mpa, and the concentrated solution has no organic solvent residue.

Example 8

The Atractylodes Rhizoma Atractylodes Extract obtained in Example 1-7 is applied to the high-fat rat model prepared by high-sugar and high-fat plus alcohol, and detects the levels of TC, LDL-C, AI, and HDL-C in the rat model after long-term administration. Variety. Specific steps are as follows:

1 Materials and Instruments

1.1 Animals: SD rats, half male and half male, weighing 180-220 g, 40 rats. Certificate number: SCXK (Zhejiang) 2008-0033

1.2 Drug preparation: Mix the extracts obtained in Examples 1-7 with emulsifiers at a mass ratio of 1:20 to obtain 7 groups of drugs, which are A1, A2, A3, A4, A5, A6, and A7.

1.3 Instruments Electronic balance CP114 (Shanghai Ohaus Instrument Co., Ltd.); YP15KN-electronic weighing balance (Shanghai Hengping Scientific Instrument Co., Ltd.); HA220-40-12 supercritical extraction device (Jiangsu Nantong Huaan Supercritical Extraction Co., Ltd. ); YF-40 traditional Chinese medicine decoction machine (Beijing Huadongyuan Medical); SHB-B95A circulating water multi-purpose vacuum pump (Shanghai Yukang Science and Education Instrument Equipment Co., Ltd.); GG-17 rotary evaporator (Shanghai Yukang Science and Education Instrument Equipment Co., Ltd. Company); lifting constant temperature water bath (Hangzhou David Science and Education Instrument Co., Ltd.) DLSB-30/30 ℃ low temperature cooling circulation pump (Hangzhou Huichuang Instrument Equipment Co., Ltd.); CM-230 pure water instrument (Hangzhou Yongjieda Purification Technology Co., Ltd. ); KQ-500DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); 501-constant temperature water oil bath, SHB-Ⅲ circulating water vacuum pump, DLSB-﹣5/10℃ low temperature cooling circulating pump (Hangzhou Huichuang Instrument Equipment Co., Ltd.); TBA-A0FR (ACCUTE) automatic biochemical analyzer (Toshiba Sanguang Medical Co., Ltd., Japan); Powerwave340 microplate reader (Bio-TEK, USA), etc.

2 Experimental methods

2.1 Administration: Before administration, blood was taken to measure the blood lipid level of the rats, which was used as the pre-administration data. According to blood lipid (mainly by TC) base value, 90 model rats were randomly divided into 9 groups, 10 in every group, wherein 7 groups were administered with A1, A2, A3, A4, A5, A6, A7 respectively, and another 1 Group 1 was set as the control group, and group 1 was set as the model group. Purified water was given normally, the emulsifier solution was given to the model group, and corresponding drugs were given to the treatment group.

2.2 Observation indicators: After the first administration on the 4th week, fast for 12 hours, take blood from the orbit, and measure biochemical indicators such as serum blood lipids.

2.3 Statistics and analysis: measurement data results and Indicates the difference between groups using t-test.

3 Experimental results

3.1 After 4 weeks of administration, the blood lipid levels of A1-A7 in the normal group, model group and each administration group

Compared with the normal group, the TC, LDL-c, and atherosclerosis index AI of the model caused by drinking high sugar and high fat were significantly increased (P<0.01), and HDL-c was significantly decreased (P<0.01); continuous administration for 4 weeks Compared with the model group, the Atractylodes macrocephala supercritical CO ₂ extract group and petroleum ether extract group can significantly reduce model rat TC, LDL-c, atherosclerosis index AI (P<0.01), and significantly increase model rat HDL _-c (P<0.01). The results are shown in Table-1.

Table 1 Effects of continuous administration for 4 weeks on blood lipids in rats

Note: Compared with the normal control group, ^△ P<0.05, ^△△ P<0.01; compared with the model control group, *P<0.05, **P<0.01

In summary, a certain dose of Atractylodes macrocephala extract of the present invention can significantly reduce total cholesterol (TC), low-density lipoprotein (LDL-C), atherosclerosis index (AI) of model rats, increase high-density lipoprotein (HDL-C), has the function of regulating blood lipids.

The above-mentioned embodiments are used to illustrate the present invention, rather than to limit the present invention. Within the spirit of the present invention and the protection scope of the claims, any modification and change made to the present invention will fall into the protection scope of the present invention.

Claims (3)

1. a kind of Rhizoma Atractylodis Macrocephalae extract with regulating blood lipid action, it is characterised in that the extract passes through CO₂Supercritical extract Obtain, comprise the following steps that：

（1）Take bighead atractylodes rhizome root medicinal material to pulverize and sieve, be put into extractor；

（2）Extracted at 20~30MPa, 40~55 DEG C, obtain extracting product；

（3）By the extraction product that step 2 obtains in 4MPa~9MPa, 40~70 DEG C are separated, and are obtained the first extract and are divided From product；

（4）The separation product that step 3 is obtained, in 3~6MPa, 30~50 DEG C are separated again, obtain the second extract；

（5）The second extract that the first extract and step 4 that collection step 3 obtains obtain, as with regulating blood lipid action Rhizoma Atractylodis Macrocephalae extract；

The step 2-4 is in 55~70L/h CO₂Carried out in medium.

2. a kind of Rhizoma Atractylodis Macrocephalae extract with regulating blood lipid action, it is characterised in that the extract is obtained by following steps：

（1）After bighead atractylodes rhizome root medicinal material is crushed, the h of organic solvent for ultrasonic 0.5~3 is added to, wherein bighead atractylodes rhizome root medicinal material is dense Spend for 0.125 ~ 0.33g/L；Refluxing extraction 1~4 time, 0.5~8.0 h every time, filtered after each refluxing extraction, filtered Liquid；

（2）Filtrate in combining step 1, is concentrated under reduced pressure, 20~55 DEG C of thickening temperature, and vacuum is -0.07~-0.09Mpa, dense Contracting liquid to organic solvent-free remains；The organic solvent is petroleum ether, n-hexane, ethyl acetate or acetone.

A kind of 3. application of Rhizoma Atractylodis Macrocephalae extract of claim 1 or 2 in the medicine for preparing prevention and treatment hyperlipidemia.