CN104396551A - Method for ecological restoration of soil in rare earth mining area by using herbaceous plants - Google Patents
Method for ecological restoration of soil in rare earth mining area by using herbaceous plants Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for restoring soil ecology in a rare earth mining area by utilizing herbaceous plants, which comprises the following steps: a. soil improvement: applying animal wastes in the soil of the mined rare earth mining area, wherein the application amount of the animal wastes is 130-170kg/100m2(ii) a b. Vegetation restoration: b, applying seeds of herbaceous plants to the soil treated in the step a, wherein the herbaceous plants are stylosanthes guianensis, phaseolus vulgaris, lucerne, vetiver grass, crabgrass and agaricus bisporus; then, the herbaceous plant seeds grow naturally to form vegetation. The method for restoring the soil ecology of the rare earth mining area by using the herbaceous plants can improve the number and the types of bacteria in the mined soil of the rare earth mining area and improve the soil ecology.
Description
Technical field
The present invention relates to rare-earth mining area soil ecology and repair field, be specifically related to a kind of method utilizing herbaceous plant to repair in rare-earth mining area soil ecology.
Background technology
China's rare earth mining site aboundresources, rare earth resources reserves, output, sales volume and usage amount all account for the first in the world, are the large rare earth resources states of the first in the world.For a long time, China is exploited a lot of rare-earth mining area without limit, and cause mining soil structural deterioration, fertility reduces, organic famine, vegetation is difficult to growth.In order to solve Rare-earth Industry sustainable development, Information Office of the State Council of China issues " China rare earth situation and policy " white paper, to strengthen the dynamics of China's rare earth resources and environmental protection in June, 2012.Particularly in March, 2014 World Trade Organization's ruling China rare earth restriction case is lost a lawsuit, China's Rare Earth Mine exploitation is described, utilize and the task of repairing very arduous, rare-earth mining area how exploitation limit, limit improvement becomes the vital task of the Rare Earth Mine sustainable development of China.
Because external rare earth resources relatively lacks, can be less for the recovery scenario of China's reference.Therefore, the vegetation ecological reparation of south China rare-earth mining area mostly in recent years is recovery scenario and the research of autonomous innovation, and attempts in mechanism level, from soil structure, heavy metal and physicochemical property angularly analyzing influence mining area vegetation growth factor.As the research of rare earth mining site vegetation repair has been carried out in Heping County, Guangdong Province, Fujian Province's Changting County, Xinfeng County, Jiangxi Province, Mianning County, Sichuan Province, all filter out the vegetable material that applicable mining area is repaired, and obtained the recovery scenario of certain maturity.Also the impact having part research to carry out soil stress factors to grow Paspalum conjugatum, tomato is to the simulated experiment of the tolerance of rare earth element, and the impact utilizing arbuscular mycorrhiza (MyeorrhizalFungi) to reduce rare earth heavy metal on plants to poison.Although these research and utilization mining area field trials and constructed experiment establish the technical scheme of a set of vegetation repair, certain shortcoming is also existed for the diversity of bacterial flora in soil and the raising of kind.
Summary of the invention
The object of the invention is to the defect overcoming above-mentioned prior art, provide the bacteria's numbers and species in the rare-earth mining area soil after can promoting exploitation, improve a kind of method utilizing herbaceous plant to repair in rare-earth mining area soil ecology of soil ecology.
For solving the problem, the technical solution adopted in the present invention is as follows:
Utilize the method that herbaceous plant is repaired in rare-earth mining area soil ecology, it comprises the steps:
A. soil melioration: apply animal wastes in the soil of the rare-earth mining area after exploitation, the applied amount of described animal wastes is 130-170kg/100m
2;
B. vegetation repair: execute in the soil through a step process and broadcast herbal seed, described herbaceous plant is khuskhus, mountain green soy bean, pigeonpea, alfalfa, vetiver, lady's-grass grass and Paspalum conjugatum; Then, allow herbaceous plant seeds self-sow, form vegetation.
In the present invention, preferred scheme is the animal wastes in described a step is chicken manure.Preferred scheme is the applied amount of described chicken manure is further 150kg/100m
2.
In the present invention, preferred scheme is the time of described herbaceous plant seeds self-sow be at least 1 year.Further preferred scheme is the time of described herbaceous plant seeds self-sow be at least 3 years.
In the present invention, preferred scheme is that the amount of broadcasting of executing of herbaceous plant seeds in described b step is respectively: Stylo seed 0.1-0.3kg/100m
2, mountain green soy bean seed 0.3-0.5kg/100m
2, Cajanus cajan seeds 0.3-0.4kg/100m
2, alfalfa seed 0.2-0.4kg/100m
2, vetiver seed 0.1-0.3kg/100m
2, lady's-grass grass seed 0.1-0.3kg/100m
2with Paspalum conjugatum seed 0.1-0.3kg/100m
2.Further preferably, Stylo seed 0.2kg/100m
2, mountain green soy bean seed 0.5kg/100m
2, Cajanus cajan seeds 0.3kg/100m
2, alfalfa seed 0.3kg/100m
2, vetiver seed 0.2kg/100m
2, lady's-grass grass seed 0.1kg/100m
2with Paspalum conjugatum seed 0.3kg/100m
2.
In the present invention, preferred scheme is that described Stylo seed is 15-20cm according to line-spacing, arranges and carry out executing broadcasting apart from for 20-30cm; Described alfalfa seed is 20-30cm according to line-spacing, arranges and carry out executing broadcasting apart from for 30-40cm; Described vetiver seed is 8-12cm according to line-spacing, arranges and carry out executing broadcasting apart from for 8-12cm; Described lady's-grass grass seed is 8-12cm according to line-spacing, arranges and carry out executing broadcasting apart from for 10-15cm; Described Paspalum conjugatum seed is 10-15cm according to line-spacing, arranges and carry out executing broadcasting apart from for 10-15cm.Further preferably, described Stylo seed is 18cm according to line-spacing, arranges and carry out executing broadcasting apart from for 25cm; Described alfalfa seed is 25cm according to line-spacing, arranges and carry out executing broadcasting apart from for 35cm; Described vetiver seed is 10cm according to line-spacing, arranges and carry out executing broadcasting apart from for 12cm; Described lady's-grass grass seed is 10cm according to line-spacing, arranges and carry out executing broadcasting apart from for 15cm; Described Paspalum conjugatum seed is 13cm according to line-spacing, arranges and carry out executing broadcasting apart from for 14cm.
Compared with prior art, advantage of the present invention is: adopt the solution of the present invention, entered the improvement of soil, and the herbaceous plant seeds self-sow of sowing, forms preparation, completes the restoration of the ecosystem of soil; Through repairing, the bacterial clump in the middle of the rare-earth mining area soil of exploitation and quantity all obtain larger lifting, and soil ecology obtains and significantly improves and recover.
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
Accompanying drawing explanation
Fig. 1 is the rare-earth mining area soil remediation procedure chart in embodiment 1;
Rare-earth mining area soil bacteria SRAP diversity variation diagram in Fig. 2 embodiment 1;
The rare-earth mining area soil bacteria cluster analysis figure based on SRAP in Fig. 3 embodiment 1;
The monoid variation diagram of the different genetic distance threshold value of the rare-earth mining area soil bacteria in Fig. 4 embodiment 1;
Rare-earth mining area soil bacteria principal component relation Butut in Fig. 5 embodiment 1;
Rare-earth mining area nocardia classification Wien graph of a relation in Fig. 6 embodiment 1;
The dynamic change figure of the rare-earth mining area nocardia ratio in Fig. 7 embodiment 1;
Wherein, in Fig. 1, a is soil surface complexion before treatment, and b-d is respectively the soil surface complexion of process First Year to the 3rd year;
In Fig. 2, wherein a is before exploitation, b is after exploitation, c-e is respectively the different times of repair process First Year, Second Year and the 3rd year vegetation repair; Na is fragment observed number, ne is effective segments, h is nei's gene diversity index, and I is shannon index;
In Fig. 3, abscissa is the genetic distance in soil between bacterium, before a-e represents rare-earth mining area exploitation respectively, exploits different times that is rear and continuous 3 years (repair First Year, repair Second Year and repair the 3rd year) vegetation repair; Numerical value in figure after any one letter of alphabetical a-e is genetic distance threshold values;
In Fig. 4, before a-e represents rare-earth mining area exploitation respectively, exploit different times that is rear and continuous 3 years (repair First Year, repair Second Year and repair the 3rd year) vegetation repair;
In Fig. 5, before a-e represents rare-earth mining area exploitation respectively, exploit soil bacteria that is rear and continuous 3 years (repair First Year, repair Second Year and repair the 3rd year) vegetation repair; I and II is different soil bacteria monoids respectively; PC-1 and PC-2 is first principal component and Second principal component, respectively;
In Fig. 6, before a-e represents rare-earth mining area exploitation respectively, exploit different times that is rear and continuous 3 years (repair First Year, repair Second Year and repair the 3rd year) vegetation repair;
In Fig. 7, before a-e represents rare-earth mining area exploitation respectively, exploit different times that is rear and continuous 3 years (repair First Year, repair Second Year and repair the 3rd year) vegetation repair.
Embodiment
Embodiment 1
Utilize the method that herbaceous plant is repaired in rare-earth mining area soil ecology, it comprises the steps:
A. soil melioration: apply chicken manure in the soil of the rare-earth mining area after exploitation, the applied amount of described animal wastes is 150kg/100m
2; Rare-earth mining area is wherein get off rare-earth mining area, town 815 in Heping County, Guangdong Province;
B. vegetation repair: execute in the soil through a step process and broadcast herbal seed, described herbaceous plant is khuskhus, mountain green soy bean, pigeonpea, alfalfa, vetiver, lady's-grass grass and Paspalum conjugatum; Then, allow herbaceous plant seeds self-sow 3 years, form vegetation;
The amount of broadcasting of executing of the herbaceous plant seeds in described b step is respectively: Stylo seed 0.2kg/100m
2, mountain green soy bean seed 0.5kg/100m
2, Cajanus cajan seeds 0.3kg/100m
2, alfalfa seed 0.3kg/100m
2, vetiver seed 0.2kg/100m
2, lady's-grass grass seed 0.1kg/100m
2with Paspalum conjugatum seed 0.3kg/100m
2; Described Stylo seed is 18cm according to line-spacing, arranges and carry out executing broadcasting apart from for 25cm; Described alfalfa seed is 25cm according to line-spacing, arranges and carry out executing broadcasting apart from for 35cm; Described vetiver seed is 10cm according to line-spacing, arranges and carry out executing broadcasting apart from for 12cm; Described lady's-grass grass seed is 10cm according to line-spacing, arranges and carry out executing broadcasting apart from for 15cm; Described Paspalum conjugatum seed is 13cm according to line-spacing, arranges and carry out executing broadcasting apart from for 14cm.
In 3 years that start from sowing, gather annual mid-June this through the inventive method carry out repair process soil specimen, with the soil be not damaged near mining area for contrast (representative exploitation before soil) and repair process before soil-like totally 5 samples of (representative exploit after soil).Soil specimen sampling area area 1000m
2, every 30m
2if 1 sample point, gathers soil specimen with aseptic shovel respectively at the soil layer of 0-20cm.Each sample point fetches earth 500g, fully uses aseptic plastic bag packing after mixing, then carries out bacterial culture separation in the lab.
Get the above-mentioned sample collected, then the Phylogenetic diversity of bacteria in sample tested, specific experiment method and result as follows:
1. method
1.1 bacteria distribution and DNA extract
Before taking rare-earth mining area exploitation, after exploitation, repair First Year, repair Second Year and repair the 3rd year totally five parts of soil samples, every part of 0.5g, is respectively charged into 10ml sterile centrifugation tube, adds 5ml redistilled water and fully dilutes.Often kind a dilution suspension is drawn 200 μ l and is coated with on solid culture medium evenly.Flat board is put 35 DEG C of constant temperature culture carton upside downs and is cultivated 12-24h.Distinguish the soil list bacterium colony 24 in picking each period again, in LB liquid nutrient medium, 12-24h are cultivated in 35 DEG C of constant incubators concussions.DNA of bacteria extracting method deliver with reference to Liu Wen paper (Liu Wen, Ke Huipeng, Liang Hong. animals and plants total nucleic acid extracts test. Zhongkai University of Agriculture And Technology, 2008,21 (3): 17-21.) in total nucleic acid extracting method.
1.2. bacterium SRAP molecular marker analysis
Paper (the Li Chunnan that SRAP amplimer is delivered with reference to Li Chunnan, Cui Hairui, Wang Wei wins. with the genetic diversity of SRAP marker research rhizosphere soil microorganism. and bio-diversity, 2011,19 (4): 485-493.) method in, selects F1R3, F1R8, F2R3, F2R4, F5R3, F5R7 six groups of primer pairs and respectively organizes sample and carry out pcr amplification (referring to following table 1).Amplification condition is as follows: in the reaction system of 25ul, 10 × Buffer 2.5 μ l, MgCl
21.5 μ l, dNTPs 1 μ l, each 0.5 μ l of primer, Taq enzyme 0.5 μ l, ddH
2o mends to 25 μ l; Amplification program is: 94 DEG C of 5min; 94 DEG C of 45s; 35 DEG C of 1min, 72 DEG C of 1min30s, 5 circulations; 94 DEG C of 45s, 50 DEG C of 1min, 72 DEG C of 1min30s, totally 35 circulations; 72 DEG C extend 10min, 4 DEG C of preservations.
Amplification sample electrophoresis in 6% polyacrylamide gel, 0.1%AgNO
3middle dyeing, the film after development (sodium tetraborate 0.076g+NaOH 6g+1.6ml formaldehyde, adds water to 400ml) carries out Taking Pictures recording with white light in gel imaging system.
1.3 bacterial 16 S rDNA gene magnification and order-checkings
The primer reference table 1 of bacterial 16 S rDNA sequence.PCR reaction system is: 10 × Buffer 5 μ l, MgCl
22.4 μ l, dNTPs 2 μ l, each 1 μ l of primer, Taq enzyme 1 μ l, ddH
2o mends to 50 μ l.Response procedures is 95 DEG C of denaturation 4min, 94 DEG C of sex change 45s, 57 DEG C of annealing 45s, and 72 DEG C extend 2min, circulate 30 times, and last 72 DEG C extend 10min, 4 DEG C of preservations.Get 3 μ l after sample amplification to detect in 1.5% agarose gel electrophoresis, after electrophoresis result display object band is clear and legible, send the order-checking of prompt base (invitrogen, the Shanghai) trade Co., Ltd in the English Weihe River.
Table 1 primer noun and sequence
1.4 data statistics and analysis
Polymorphism analyzing method: calculate pleomorphism site percentage and polymorphism information amount, band clearly on statistics gel, person's assignment is 1 band, is 0 without band person assignment, thus formation raw data matrix; Pleomorphism site percentage (PPB)=polymorphism bar algebraically/total band number × 100%.POPGENE 32 software is utilized to calculate fragment observed number (na), effectively segments (ne), Nei's gene diversity (h), shannon index (I); Using Piccal software to calculate polymorphism information content (Polymorphism information content, PIC) computing formula is PIC=1-∑ (Pi)
2; Cluster analysis is carried out according to Neis' genetic identity in population with NTSYS2.10 software; SPSS17.0 software calculates first principal component and the Second principal component, of bacterial clump; The 16SrDNA sequence application DNA Star software measured carries out sequence assembly, spliced order-checking is carried out Blast comparison in ncbi database, obtains homology alignment's result, thus identify the bacterium kind in the soil of rare-earth mining area; The Vean diagram (Venn diagrams) of bacterium genus classification calculates and picture utilizes online website tools analysis (http://bioinformatics.psb.ugent.be/webtools/Venn/).
2. results and analysis
The dynamic change of 2.1 rare-earth mining area vegetation repair processes
What 815 ore deposit points adopted is ammonium sulfate extraction extracting rare-earth element, and mining area is after man-made recovery, and soil structure and composition are subject to heavy damage, and mining area does not almost have plant species to retain, long-term exposed of soil cause serious water and soil loss (Fig. 1, a).This research adopts and use dried poultrymanure in mining area, and execute the seed broadcasting khuskhus, mountain green soy bean and pigeonpea, alfalfa, vetiver, lady's-grass grass and Paspalum conjugatum, rare-earth mining area vegetal cover degree after exploitation is increased year by year, dense vegetation is covered when reparation the 3rd year, significantly improve ecological condition (Fig. 1, b-d).
2.2 soil bacteria SRAP polymorphism analysis
The DNA sample of the bacterium of the rare-earth mining area soil before and after the reparation of 6 pairs of SRAP primer pairs is selected to carry out pcr amplification product polyacrylamide gel electrophoresis, DNA band on statistics gel, utilize POPGENE32 software to calculate amplification number of sites, polymorphic position are counted, polymorphism percentage, PIC value, na, ne, h and I.Result shows, and along with exploitation and the repair process of rare-earth mining area, 8 diversity index all there occurs dynamic change.Amplification number of sites, polymorphic position is counted and na tri-diversity index obviously decline after mining, after the reparation of First Year, before desired value has exceeded exploitation, and increases year by year; Polymorphism percentage and PIC value then show as the trend increased year by year; Ne, h and I tri-diversity index obviously decline after mining, and along with vegetation repair, First Year and Second Year present increase trend, but until the reparation that have passed through 3 years, before diversity index just exceedes exploitation.Therefore, from 8 diversity index, the exploitation of Rare Earth Mine significantly reduces soil bacteria diversity, after being repaired by artificial vegetation, diversity index rises year by year, and through the vegetation repair of 3 years, soil bacteria diversity was even higher than (the referring to Fig. 2) before exploitation.
By the genetic cluster analysis of the soil bacteria in each time, the genetic distance between discovery soil bacteria, between 0.60-0.93, can be divided into different monoids (referring to Fig. 3) in different genetic distance threshold values.The threshold value that this research have chosen 0.70,0.75 and 0.80 three genetic distance compares, and find that the exploitation of Rare Earth Mine reduces mining soil bacterial groups, after vegetation repair, soil bacteria monoid number has rising to a certain degree (referring to Fig. 4).
By analyzing mining soil bacterium first principal component and Second principal component, distribution map, find that the bacterium of different year is divided into two large monoids.First monoid is the main body monoid of soil bacteria, includes different year soil bacteria, account for most soil bacteria (Fig. 5, I); Second monoid comprises 2 and repairs the soil bacteria of First Years, and the soil bacteria (Fig. 5, II) of 1 and 5 reparation Second Years and the 3rd year.Illustrate after artificial vegetation repairs, occurred new bacteria types in soil, these new bacteria types may be artificial vegetation's repair processes or come from surrounding environment transfer shift-in.
2.3 bacterium 16s rDNA diversity analysis
16S rDNA sequence can be used to the Phylogenetic Relationships inferring bacterium, can identify unknown bacterium with database after comparing.In the present invention, by the soil separation of bacterial bacterium colony of different phase, carry out 16S rDNA and check order, sequence is carried out Blast compare of analysis in ncbi database.Wien graph of a relation is built after sequence B last comparison, result shows, rare-earth mining area isolates 8 bacteriums belonged to altogether, wherein 2 bacteriums are had to belong to (Arthrobacter and Bacillus) before the exploitation of rare-earth mining area, after exploitation, after repairing, first average annual belongs to (Brevibacillus) containing 1 bacterium, after Second Year and the 3rd year vegetation repair, 4 kinds of (Bacillus have been separated respectively in the present invention, Bacterium, Lysinibacillus and Sinorhizobium) and 5 kinds of (Paenibacillus, Bacillus, Sinorhizobium, Solibacillus and Bacterium) bacterium genus (referring to Fig. 6).Change environment and the bacteria live condition of soil after the exploitation of visible rare-earth mining area, the bacterium in primary border is disappeared.Through the reparation of 3 years, the kind that the bacterium in soil belongs to obviously increased, and illustrated that vegetation repair has a significant effect for maintenance soil bacterial community diversity.
Compare the ratio (referring to Fig. 7) of each time mining soil bacterium, find that the sociales before exploitation are respectively Arthrobacter and Bacillus; Brevibacillus is with the bacterium of repairing rear First Year after exploitation; After repairing Second Year and the 3rd year appearance exploitation before predominant bacteria belong to Bacillus, account for that bacterium belongs to total amount respectively 80% and 40%.Before exploitation, the bacterium of repairing Second Year and the 3rd year belongs to ratio, and the bacterium that Bacillus belongs to accounts for as advantage under better ecological environmental condition, and the bacterium that Brevibacillus belongs to accounts for as advantage when ecological condition is poor.
The result of variations display of soil bacteria diversity and kind in the rare-earth mining area soil remediation process of process of the present invention, after mining area exploitation, soil environment is damaged, Phylogenetic diversity of bacteria and bacterial species decline, through soil melioration and vegetation repair, soil bacteria genetic diversity and kind increase year by year, the majority parameters of repairing rear second and third year soil bacteria has been greater than the front soil rear with exploitation of exploitation, reflect vegetation repair not only obvious to the effectiveness comparison of rare-earth mining area soil environment recovery, and maintain the genetic diversity of higher bacterial community.
Above-mentioned embodiment is only the preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, change and the replacement of any unsubstantiality that those skilled in the art does on basis of the present invention all belong to the present invention's scope required for protection.
Claims (9)
1. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology, is characterized in that comprising the steps:
A. soil melioration: apply animal wastes in the soil of the rare-earth mining area after exploitation, the applied amount of described animal wastes is 130-170kg/100m
2;
B. vegetation repair: execute in the soil through a step process and broadcast herbal seed, described herbaceous plant is khuskhus, mountain green soy bean, pigeonpea, alfalfa, vetiver, lady's-grass grass and Paspalum conjugatum; Then, allow herbaceous plant seeds self-sow, form vegetation.
2. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 1, is characterized in that: the animal wastes in described a step are chicken manure.
3. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 2, is characterized in that: the applied amount of described chicken manure is 150kg/100m
2.
4. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 1, is characterized in that: the time of described herbaceous plant seeds self-sow is at least 1 year.
5. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 4, is characterized in that: the time of described herbaceous plant seeds self-sow is at least 3 years.
6. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 1, is characterized in that the amount of broadcasting of executing of the herbaceous plant seeds in described b step is respectively: Stylo seed 0.1-0.3kg/100m
2, mountain green soy bean seed 0.3-0.5kg/100m
2, Cajanus cajan seeds 0.3-0.4kg/100m
2, alfalfa seed 0.2-0.4kg/100m
2, vetiver seed 0.1-0.3kg/100m
2, lady's-grass grass seed 0.1-0.3kg/100m
2with Paspalum conjugatum seed 0.1-0.3kg/100m
2.
7. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 6, is characterized in that: Stylo seed 0.2kg/100m
2, mountain green soy bean seed 0.5kg/100m
2, Cajanus cajan seeds 0.3kg/100m
2, alfalfa seed 0.3kg/100m
2, vetiver seed 0.2kg/100m
2, lady's-grass grass seed 0.1kg/100m
2with Paspalum conjugatum seed 0.3kg/100m
2.
8. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 1, is characterized in that the amount of broadcasting of executing of the herbaceous plant seeds in described b step is respectively: described Stylo seed is 15-20cm according to line-spacing, arranges and carry out executing broadcasting apart from for 20-30cm; Described alfalfa seed is 20-30cm according to line-spacing, arranges and carry out executing broadcasting apart from for 30-40cm; Described vetiver seed is 8-12cm according to line-spacing, arranges and carry out executing broadcasting apart from for 8-12cm; Described lady's-grass grass seed is 8-12cm according to line-spacing, arranges and carry out executing broadcasting apart from for 10-15cm; Described Paspalum conjugatum seed is 10-15cm according to line-spacing, arranges and carry out executing broadcasting apart from for 10-15cm.
9. the method utilizing herbaceous plant to repair in rare-earth mining area soil ecology according to claim 8, is characterized in that the amount of broadcasting of executing of the herbaceous plant seeds in described b step is respectively: described Stylo seed is 18cm according to line-spacing, arranges and carry out executing broadcasting apart from for 25cm; Described alfalfa seed is 25cm according to line-spacing, arranges and carry out executing broadcasting apart from for 35cm; Described vetiver seed is 10cm according to line-spacing, arranges and carry out executing broadcasting apart from for 12cm; Described lady's-grass grass seed is 10cm according to line-spacing, arranges and carry out executing broadcasting apart from for 15cm; Described Paspalum conjugatum seed is 13cm according to line-spacing, arranges and carry out executing broadcasting apart from for 14cm.
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| CN110583374A (en) * | 2019-10-09 | 2019-12-20 | 江西省核工业地质局二六四大队 | Method for repairing waste rare earth mine based on paspalum cultivation |
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Application publication date: 20150311 |