CN104387273A - Method for extracting chlorogenic acid from honeysuckle - Google Patents
Method for extracting chlorogenic acid from honeysuckle Download PDFInfo
- Publication number
- CN104387273A CN104387273A CN201410555430.XA CN201410555430A CN104387273A CN 104387273 A CN104387273 A CN 104387273A CN 201410555430 A CN201410555430 A CN 201410555430A CN 104387273 A CN104387273 A CN 104387273A
- Authority
- CN
- China
- Prior art keywords
- honeysuckle
- chlorogenic acid
- extracting
- concentrated solution
- stems
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting chlorogenic acid from honeysuckle. The method comprises the following steps: getting stems and leaves of the honeysuckle and adopting an extracting liquid which is five times of the stems and leaves of the honeysuckle in total volume and is 60% in content; heating a mixture of the stems and leaves of the honeysuckle and the extracting liquid to the temperature of about 90 DEG C till boiling; using a back flowing device to carry out back flow for 3 h; carrying out extraction twice, merging the liquids obtained through twice extraction, and carrying out filtration to obtain paste; using water to dilute the paste to obtain a concentrated solution; filtering the concentrated solution, carrying out vacuum concentration till the filtered concentrated solution becomes dry to obtain the chlorogenic acid. Through a plurality of steps provided by the method, the chlorogenic acid can be effectively extracted from the honeysuckle. The method is high in extraction rate and less in raw material waste.
Description
Technical field
The present invention relates to the raw material extracting method in a kind of plant, particularly relate to a kind of method of chlorogenic acid extracting from Japanese Honeysuckle.
Background technology
The amount of Japanese Honeysuckle each organ Content of Chlorogenic Acid is very abundant, and the chlorogenic acid material in Japanese Honeysuckle is mainly accumulated in its cauline leaf and reproductive organ, and the amount in root is little.In the growth course of cauline leaf, the chlorogenic acid amount of full-bloom stage is the highest, and amount during setting declines to some extent, and amount during fruit maturation starts again to rise, and when withered early stage, the chlorogenic acid amount in both returns to again a higher level.How to carry out extraction to the chlorogenic acid in Japanese Honeysuckle is a research emphasis.
Summary of the invention
Object of the present invention is just a kind of method providing chlorogenic acid extracting from Japanese Honeysuckle in order to solve the problem.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A method for chlorogenic acid extracting from Japanese Honeysuckle, comprises the following steps:
Extracting honeysuckle cauline leaf, employing cumulative volume is the content of Japanese Honeysuckle cauline leaf 5 times is the extraction liquid of 60%, at the heating temperatures of about 90 degree to boiling, adopt reflux, reflux 3 hours, extract 2 times altogether, the extracting solution extracted 2 times merges, and obtains lotion, by lotion dilute with water after filtering, concentrated solution is filtered, is evaporated to do and both must.The present invention is achieved by multiple step and effectively extracts the chlorogenic acid in Japanese Honeysuckle, and extraction yield is high, and wastage of material is less.
Beneficial effect of the present invention is:
The invention provides a kind of method of chlorogenic acid extracting from Japanese Honeysuckle, achieved by multiple step and effectively extract the chlorogenic acid in Japanese Honeysuckle, extraction yield is high, and wastage of material is less, has the value promoted the use of.
Embodiment
A method for chlorogenic acid extracting from Japanese Honeysuckle, comprises the following steps:
Extracting honeysuckle cauline leaf, employing cumulative volume is the content of Japanese Honeysuckle cauline leaf 5 times is the extraction liquid of 60%, at the heating temperatures of about 90 degree to boiling, adopt reflux, reflux 3 hours, extract 2 times altogether, the extracting solution extracted 2 times merges, and obtains lotion, by lotion dilute with water after filtering, concentrated solution is filtered, is evaporated to do and both must.The present invention is achieved by multiple step and effectively extracts the chlorogenic acid in Japanese Honeysuckle, and extraction yield is high, and wastage of material is less.
Technical scheme of the present invention is not limited to the restriction of above-mentioned specific embodiment, the technology distortion that every technical scheme according to the present invention is made, and all falls within protection scope of the present invention.
Claims (1)
1. the method for chlorogenic acid extracting from Japanese Honeysuckle, is characterized in that, comprise the following steps:
Extracting honeysuckle cauline leaf, employing cumulative volume is the content of Japanese Honeysuckle cauline leaf 5 times is the extraction liquid of 60%, at the heating temperatures of about 90 degree to boiling, adopt reflux, reflux 3 hours, extract 2 times altogether, the extracting solution extracted 2 times merges, and obtains lotion, by lotion dilute with water after filtering, concentrated solution is filtered, is evaporated to do and both must.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410555430.XA CN104387273A (en) | 2014-10-20 | 2014-10-20 | Method for extracting chlorogenic acid from honeysuckle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410555430.XA CN104387273A (en) | 2014-10-20 | 2014-10-20 | Method for extracting chlorogenic acid from honeysuckle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104387273A true CN104387273A (en) | 2015-03-04 |
Family
ID=52605252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410555430.XA Pending CN104387273A (en) | 2014-10-20 | 2014-10-20 | Method for extracting chlorogenic acid from honeysuckle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104387273A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101838200A (en) * | 2009-03-20 | 2010-09-22 | 上海中医药大学 | Method for extracting and separating chlorogenic acid from honeysuckle |
| CN102443619A (en) * | 2010-10-09 | 2012-05-09 | 苏州宝泽堂医药科技有限公司 | Method for extracting chlorogenic acid and hederagenin from honeysuckle flower |
| CN104069150A (en) * | 2014-07-18 | 2014-10-01 | 鲁南制药集团股份有限公司 | Preparation method for honeysuckle extract |
-
2014
- 2014-10-20 CN CN201410555430.XA patent/CN104387273A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101838200A (en) * | 2009-03-20 | 2010-09-22 | 上海中医药大学 | Method for extracting and separating chlorogenic acid from honeysuckle |
| CN102443619A (en) * | 2010-10-09 | 2012-05-09 | 苏州宝泽堂医药科技有限公司 | Method for extracting chlorogenic acid and hederagenin from honeysuckle flower |
| CN104069150A (en) * | 2014-07-18 | 2014-10-01 | 鲁南制药集团股份有限公司 | Preparation method for honeysuckle extract |
Non-Patent Citations (1)
| Title |
|---|
| 蒋玉林等: "HPLC分析比较金银花花茎叶中绿原酸含量", 《广东化工》 * |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150304 |
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| RJ01 | Rejection of invention patent application after publication |