CN104382825A - Extraction method of micromolecular sheep placenta extract and application of micromolecular sheep placenta extract in field of skin-care products - Google Patents
Extraction method of micromolecular sheep placenta extract and application of micromolecular sheep placenta extract in field of skin-care products Download PDFInfo
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Abstract
The invention discloses an extraction method of a micromolecular sheep placenta extract and application of the micromolecular sheep placenta extract in the field of skin-care products, belonging to the technical field of biology. The extraction method disclosed by the invention comprises the following steps of obtaining a lot of sheep pregnant in the same period by adopting synchronization of estrus, taking out sheep fetus after 85-100 days through an operation method to obtain body homogenate, carrying out repetitive freeze-thawing and splitting cells, degenerating at high temperature, sterilizing, precipitating hybrid protein, carrying out 30 KD ultrafiltration after centrifuging at high speed so as to remove toxic and harmful components and impurities, such as macromolecular hybrid protein, bacteria and endogenous virus, so as to obtain a pure micromolecular sheep placenta extract product, and carrying out ultrafiltration and concentration of the micromolecular sheep placenta extract product by using a 1 KD ultrafiltration component. The micromolecular sheep placenta extract disclosed by the invention can be widely used in the fields of skin-care products, beauty products, wound healing products, health-care products, scientific searches and the like; the extraction method disclosed by the invention provides a novel important way for producing the sheep placenta extract; furthermore, the compositions of the micromolecular sheep placenta extract are authenticated for the first time, and thus, theoretical foundation is provided for scientifically using the sheep placenta extract.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of extracting method of micromolecule sheep placental extract and the utilization in skin care field.
Background technology
Sheep placental extract, contains sheep placenta (the Extract of Sheep`s Placental) and sheep embryo extract (theExtract of Goat`s Embryo) two different products.
Sheep placenta is that the one in Placenta caprae seu ovis has bioactive material, is widely used in various cosmetics, medicine and health food after extracting.
Sheep embryo extract is then be present in fetal lamb body, is also be widely used in various cosmetics, medicine and health food after extracting.
From sheep embryo, extract active substance more much more difficult than extracting active substance from Placenta Hominis, also precious more expensive, the composition of active substance also differs widely.Sheep embryo extract can human activin aged cells, promote cell regeneration and division.
Switzerland scientist Ka Er in 1912 teaches in Late Cambrian sheep embryonic cell a kind of material that can make cellular-restoring vigor, this active substance can impel the increase of frequency dividing cell, can force and fail or aging cell reactivation or be eliminated, thus make the organ of hypofunction recover vigorous vigor.Therefore professor Ka Er obtains Nobel Prize.This material is described sheep embryo extract.
1931, Switzerland Borrow Ni Han doctor first a kind of active substance extracted from lamb embryo is expelled to parathyroid gland impaired be dying in patient body, and successfully saved its life, the initial origin of sheep embryo extract cell activation therapy that Here it is, its discovery has landmark meaning to the mankind at the medical development in defying age field.That is the first utilization of sheep embryo extract.
Sheep placental extract product is mainly derived from above-mentioned Placenta caprae seu ovis, sheep embryo two aspects in the market, and is used for the fields such as health product, skin care item, medicine with the form that Placenta caprae seu ovis powder, sheep embryonic powder, Placenta caprae seu ovis extract lyophilized powder, sheep embryo extract lyophilized powder etc. are different.The production method of various product is multifarious, and production method patent is also a lot, but these methods scarcely gear to actual circumstances, inadequate scientific and precise, is difficult to formal production and forms the productivity.
Applicant studies the extracting method of sheep placental extract for a long time, develop one practical, science, rigorous, the extracting method of efficient sheep embryo extract, and obtain a kind of micromolecule sheep placental extract.
Sheep placental extract of the present invention is sheep embryo extract, pass through biological technique method, the class bioactive substance extracted fetal lamb from 85-100 days, the multiple defying age factor containing maximum in the fetal lamb in this stage, epidermal growth factor, the tissue repair factor etc., can promote that epidermal differentiation upgrades, make body slow down aging, increase skin elasticity, make skin-whitening smooth, can also improving water flood, enhancing immunity, resisting fatigue, the functions such as radioprotective, overcoming current Foetus Caprae seu Ovis extract, to there is immunogenicity high, biological activity is poor, specificity is weak, the shortcomings such as DeGrain, make it have stronger adaptability, not easily irritated, better defying age, the function of Promote cell's growth and tissue repair.
The process for extracting of current sheep embryo extract is many, more complicated or too extensive, can not be used for practice.Complicated as: application number 98112918.8, a kind of manufacture method of sheep placental extract injection, adds the chemical substances such as acetone, ethanol, trichloroacetic acid, ammonium sulfate, can face a lot of problem in actual production and product practicality in manufacturing process.Simple method is a lot, have be purely the name actual production being turned on sheep placental extract be Foetus Caprae seu Ovis powder, as application number: 98101188.8, a kind of method making frozen dry extracts of sheep embryo; Application number: 98115761.0, freeze-dried sheep placenta extract powder and production method thereof.The protein peptide section that even with the addition of a large amount of pancreatin or pepsin enzyme action in the purer sheep placental extract extracted had, and these peptide sections are very complicated mixture, limit the scope of application of sheep placental extract and affect result of use, as application number: 200610085611.6, the method and uses thereof of extraction of activated micromolecular sheep placental extract from sheep embryo.
Summary of the invention
The object of the present invention is to provide a kind of extracting method of micromolecule sheep placental extract, it is exactly a kind of micromolecule sheep embryo extract, that the one extracted from the sheep embryonic cell of 85-100 days gestational ages has high bioactive nutrient substance, the split speed of human body cell can be improved, activating human body cells, the decomposition of accelerated ageing cell is got rid of.
The present invention also aims to the application providing above-mentioned micromolecule sheep placental extract in skin care field.
An extracting method for micromolecule sheep placental extract, carries out in accordance with the following steps:
(1) select healthy, age 2-3 year, the estrus cycle normal multiparity ewe as treating pregnant sheep, all sheep are all quarantined, prevent epidemic and the process such as dipping, and in feedstuff, supplement greenfeed, vitamin and trace element;
(2) at intramuscular injection Cloprostenol when pregnant sheep is at 7-14d luteal phase, start observation after 30h and oestrus, when observe oestrus after within 12 hours, carry out artificial semenpicking and semen deposition, 24 can be once composite after oestrusing, carry out ultrasound diagnosis after 35 days, determine conceived situation;
(3) when fetal lamb grows to 85-100 days, modus operandi takes out fetal lamb, is placed in the container of high temperature sterilize, cleans with physiological saline solution, removes eyeball, brain, liver, lung, the heart, intestinal stomach function regulating;
(4) with physiological saline solution cleaning, drain away the water, weigh, with the shears of sterilizing, fetal lamb is shredded, put into the refiner homogenate of sterilizing;
(5) add normal saline and fetal lamb fragment carries out homogenate, homogenate adds normal saline dilution, the total amount of normal saline be the 2-4 of fetal lamb quality doubly, homogenate diluent filters through stainless steel filtering net, upper strata 50 order, middle level 100 order, lower floor 200 order;
(6) homogenate filtered solution is placed in the safety glass pallet of sterilizing and places-30 DEG C of refrigerators, freezingly spends the night, or stores;
(7) take out frozen homogenate filtered solution thaw at RT, to complete freeze thawing, proceed in sterile centrifugation container, 3000-5000rpm, centrifugal 8-12 minute, abandons precipitation;
(8) supernatant proceeds to aseptic heatproof container, is placed in 60-80 DEG C of water-bath, and 60-80 DEG C of insulation 20-40 minute, fully kills virus and degeneration foreign protein that fetal lamb may bring into from parent;
(9) put into rapidly-30 DEG C of refrigerators and be cooled to-1-1 DEG C, or frozen;
(10) transfer liquid is to the centrifuge container of sterilizing, 3000-5000rpm, and centrifugal 20-40 minute, abandons precipitation;
(11) supernatant proceeds to sterilization container, uses cross-flow ultrafiltration system, uses different systems, ultrafiltration according to the volume of supernatant;
(12) be divided into two parts after ultrafiltration through membranes, concentrated solution is greater than the foreign protein of 30KD, abandons; Filtered solution is the small molecular protein being less than 30KD; Use cross-flow ultrafiltration system to carry out ultrafiltration and concentration to filtered solution, obtain concentrated solution and filtered solution, carry out 0.22ug filtration sterilization to concentrated solution ,-80 DEG C frozen.
The described cross-flow ultrafiltration system of step (11) is Centramate ultrafiltration system and T series membranes bag, 30KD aperture.
The described cross-flow ultrafiltration system of step (12) is Centramate ultrafiltration system and T series membranes bag, 30KD aperture, uses 1KD film bag.
Described micromolecule sheep placental extract color is faint yellow, and the denseer color of concentration is darker, and maximal ultraviolet absorption value is 252nm; PH is 6.7-7.4; SDS-PAGE electrophoretic band is 15KD; Purity 95%; Productive rate is 1g/100g fetal lamb body weight.
Above-mentioned micromolecule sheep placental extract is in the application of skin care field, the fineness of skin and the lyophilized powder of sophistication is increased for the preparation of wrinkle, described lyophilized powder is made up of the material of following parts by weight: micromolecule sheep placental extract 3-8 part, mannitol 3-7 part, trehalose 3-7 part, hyaluronic acid 0.01-1 part, water 80-90 part.
Above-mentioned micromolecule sheep placental extract is in the application of skin care field, increase the fineness of skin and the solution of sophistication for the preparation of wrinkle, described solution is made up of the material of following parts by weight: micromolecule sheep placental extract 4-8 part, mannitol 3-7 part, trehalose 3-7 part, water 80-90 part.
Beneficial effect of the present invention:
The present invention adopts physical method to divide multistagely to carry out purification to raw material; whole process does not have the intervention of organic reagent or other chemical substances; and complete extraction at low temperatures; not only maintain the pure of extract; and the active substance remained to greatest extent in Foetus Caprae seu Ovis; safety, environmental protection, low energy consumption, be easy to standardization, large-scale production.
The present invention adopts synchronization of Estrus and surgical method to operate on acquisition fetal lamb, both obtained same batch of a large amount of fetal lamb and in turn ensure that the aseptic, fresh of fetal lamb raw material, postoperative ewe can also continue pregnancy, produces new fetal lamb, improve the utilization rate of ewe, decrease cost.
The present invention adopts the technical scheme of grading extraction, Optimum utilization.After being first extracted a kind of micromolecule natural activity micromolecule sheep placental extract, again papain enzyme action being carried out to macromole sheep placental extract, be there is bioactive micromolecule polypeptide and free amino acid in a large number.Both improve the production efficiency of product, ensure that the maximum result of use of utilization and the product to greatest extent of raw material; Both maintain the natural activity of micromolecule sheep placental extract, turn avoid the problems such as the immunogenicity that macromole sheep placental extract has.
Present invention employs advanced cross-flow ultrafiltration technology, physical method has intercepted the micromolecule polypeptide after the natural activity micromolecule sheep placental extract and enzyme action being less than 30K; Use cross-flow ultrafiltration technology to concentrate simultaneously, tackle and concentrate have natural activity, main molecules amount is micromolecule sheep placental extract and the enzyme action polypeptide of 1-30K.
Present invention employs ultrasonic wave added enzyme incision technology, the macromole sheep placental extract albumen being greater than 30K carried out fast, low temperature hydrolysis, the drawback that the temperature avoiding traditional protein enzymolysis is high, the time is long.
The present invention obtains micromolecule sheep placental extract is better than similar product sheep placental extract in physicochemical property parameter and uv absorption spectra spectrum etc., and through scavenging hydroxyl, superoxide radical experiment, cell culture experiments, volunteer test, all prove its activity having very high scavenging free radicals, Promote cell's growth and remove the effect of wrinkle, beautifying skin.
The micromolecule sheep placental extract that the present invention obtains is of many uses, is applicable to the large production of industry.The advantage that raw material sources, Feedstock treating, production efficiency, production technology, product effect have traditional method incomparable, operation of easily founding the factory, and low energy consumption, low emission, meets low-carbon environment-friendly requirement.
Accompanying drawing explanation
Fig. 1 is the micromolecule sheep placental extract electrophoretogram that the present invention extracts.
Fig. 2 is for using micromolecule sheep placental extract cross-reference figure of the present invention.
Fig. 3 is for using micromolecule sheep placental extract cross-reference figure of the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
Embodiment 1
An extracting method for micromolecule sheep placental extract, carries out in accordance with the following steps:
(1) select healthy, age 2-3 year, the estrus cycle normal multiparity ewe as treating pregnant sheep, all sheep are all quarantined, prevent epidemic and the process such as dipping, and in feedstuff, supplement greenfeed, vitamin and trace element;
(2) at intramuscular injection Cloprostenol when pregnant sheep is at 7-14d luteal phase, start observation after 30h and oestrus, when observe oestrus after within 12 hours, carry out artificial semenpicking and semen deposition, 24 can be once composite after oestrusing, carry out ultrasound diagnosis after 35 days, determine conceived situation;
(3) when fetal lamb grows to 85-100 days, modus operandi takes out fetal lamb, is placed in the container of high temperature sterilize, cleans with physiological saline solution, removes eyeball, brain, liver, lung, the heart, intestinal stomach function regulating;
(4) with physiological saline solution cleaning, drain away the water, weigh, with the shears of sterilizing, fetal lamb is shredded, put into the refiner homogenate of sterilizing;
(5) add normal saline and fetal lamb fragment carries out homogenate, homogenate adds normal saline dilution, and the total amount of normal saline is 3 times of fetal lamb quality, and homogenate diluent filters through three layers of stainless steel filtering net, upper strata 50 order, middle level 100 order, lower floor 200 order;
(6) homogenate filtered solution is placed in the safety glass pallet of sterilizing and places-30 DEG C of refrigerators, freezingly spends the night, or stores;
(7) take out frozen homogenate filtered solution thaw at RT, to complete freeze thawing, proceed in sterile centrifugation container, 4000rpm, centrifugal 10 minutes, abandon precipitation;
(8) supernatant proceeds to aseptic heatproof container, is placed in 70 DEG C of water-baths, and 70 DEG C of insulations 30 minutes, fully kill virus and degeneration foreign protein that fetal lamb may bring into from parent;
(9) put into rapidly-30 DEG C of refrigerators and be cooled to-1-1 DEG C, or frozen;
(10) transfer liquid is to the centrifuge container of sterilizing, 4000rpm, centrifugal 30 minutes, abandons precipitation;
(11) supernatant proceeds to sterilization container, uses cross-flow ultrafiltration system, uses different systems, ultrafiltration according to the volume of supernatant;
(12) be divided into two parts after ultrafiltration through membranes, concentrated solution is greater than the foreign protein of 30KD, abandons; Filtered solution is the small molecular protein being less than 30KD; Use cross-flow ultrafiltration system to carry out ultrafiltration and concentration to filtered solution, obtain concentrated solution and filtered solution, carry out 0.22ug filtration sterilization to concentrated solution ,-80 DEG C frozen.
The described cross-flow ultrafiltration system of step (11) is Centramate ultrafiltration system and T series membranes bag, 30KD aperture.
The described cross-flow ultrafiltration system of step (12) is Centramate ultrafiltration system and T series membranes bag, 30KD aperture, uses 1KD film bag.
Described micromolecule sheep placental extract color is faint yellow, and the denseer color of concentration is darker, and maximal ultraviolet absorption value is 252nm; PH is 6.7-7.4; SDS-PAGE electrophoretic band is 15KD; Purity 95%; Productive rate is 1g/100g fetal lamb body weight.Its electrophoretogram as shown in Figure 1.
Embodiment 2
(1) dissect ewe by operation method, obtain aseptic fetal lamb, be placed in sterile chamber, clean with normal saline, remove eyeball, brain, liver, lung, the heart, intestinal stomach function regulating.
(2) with physiological saline solution cleaning, drain away the water, weigh.
(3) add sterile pure water and fetal lamb fragment carries out homogenate, homogenate adds pure water dilution, and the quality of pure water is 3 times of fetal lamb quality, and homogenate diluent filters through three layers of stainless steel filtering net, upper strata 20 order, middle level 50 order, lower floor 100 order.
(4) homogenate filtered solution is placed in sterile chamber and places-30 DEG C of refrigerators, freezingly spends the night, or stores.
(5) take out frozen homogenate filtered solution thaw at RT, proceed to aseptic heatproof container, be placed in 70 DEG C of water-baths, 70 DEG C are incubated 30 minutes.
(6) cool rapidly ,-30 degrees Celsius frozen.
(7) thaw, transfer liquid is to centrifuge container, and 4000rpm, 10 minutes, abandons precipitation.
(8) supernatant proceeds to sterile chamber, uses pall company cross-flow ultrafiltration system, 30k aperture, ultrafiltration.
(9) be divided into two parts after 30K ultrafiltration through membranes, a part is the large protein being greater than 30K, and a part is less than the micromolecule polypeptide of 30k, centrifugal to the component being greater than 30KD, and 4000 leave the heart 20 minutes, goes precipitation; Carry out ultrasonic wave added enzyme action with papain, 95-98 degree Celsius of insulation solution 3 ~ 5 minutes to stop enzyme digestion reaction, static 10 minutes also fast cooling lower than 10 degrees Celsius.
(10) centrifugal to said hydrolyzed liquid, 4000rpm, 20 minutes, the precipitation produced after removing enzyme action.Retain supernatant, supernatant, through the ultrafiltration of 30KD ultrafilter membrane, collects the component being less than 30KD; Re-use pall company cross-flow ultrafiltration system, 1kD aperture, the component being less than 30KD is concentrated, nucleic acid-protein analyzer (Eppendorf BioPhotometer Plus) is adopted to measure its concentration value, protein concentration pattern, 280nm detects, unified concentration.Be numbered: A2, SDS-PAGE electrophoresis detection, main band is the micromolecule polypeptide of 12K size.A2 is and optimizes micromolecule sheep placental extract stock solution, for subsequent use.
(11) the 9th step ultrafiltration is less than to the further extraction purification of component of 30K, use pall company cross-flow ultrafiltration system, 1kD aperture, ultrafiltration, two components are obtained after ultrafiltration, concentrated solution is the highly enriched product being less than 30K ultrafiltration component, nucleic acid-protein analyzer (Eppendorf BioPhotometer Plus) is adopted to measure its concentration value, protein concentration pattern, 280nm detects, unified concentration, be numbered A1, be the micromolecule polypeptide stock solution of natural activity, SDS-PAGE electrophoresis main molecules amount is at about 12KD, its molecular weight of permeate is less than 1KD, SDS-PAGE electrophoresis can not detect band.
(12) A1, A2 is frozen respectively as raw material.Thaw when need manufacture a finished product, measure the raw material of certain volume, take mannitol by need of production, 5% final concentration takes by mass percentage; Take trehalose, 5% final concentration takes by mass percentage, the two adds A1 or A2 and dissolves (final mass percentage is 6%), standardize solution, use 0.22um frit, adopt nucleic acid-protein analyzer (Eppendorf BioPhotometerPlus) to measure its concentration value, protein concentration pattern, 280nm detects, for subsequent use.
(13) sterile hyaluronic acid sodium is taken in addition, add appropriate aseptic PBS to dissolve, its concentration is made to be 1%, the hyaluronic acid solution getting appropriate 1% adds the above-mentioned sheep placental extract liquid having added adjuvant (mannitol, trehalose), the final concentration of hyaluronic acid in solution is made to be 0.05%, subpackage on superclean bench, subpackage volume 1ml/ manages.Freeze dryer lyophilizing, adds the aseptic dissolving water dissolution of 3ml, is directly applied in the position that face etc. needs beauty and skin care during use.
(14) to A1, A2 raw material according to (12), the step of (13) step is processed into finished product, obtains product 1 respectively and product 2, two kinds of products all have the activity efficiently removing hydroxy radical, superoxide radical.
A1, molecular weight is less than natural activity micromolecule polypeptide and the small protein of 30KD, main molecules amount detects main at about 12KD through SDS-PAGE, have scavenging hydroxyl, superoxide radical, Promote cell's growth activity and remove wrinkle, beautifying skin effect, Mass Spectrometric Identification goes out to have polypeptide fragment or the small protein of albumen in 54.
A2, molecular weight is less than the active small molecular polypeptide of 30KD, detects its molecular weight mainly at about 12KD through SDS-PAGE, has scavenging hydroxyl, superoxide radical is active.
This product uses through 100 volunteers, and discovery significantly can remove wrinkle, increases fineness and the sophistication of skin.Result of use is shown in Fig. 2-3 (this volunteer is women, 65 years old, artist, uses A1 product).
Claims (6)
1. an extracting method for micromolecule sheep placental extract, is characterized in that, carries out in accordance with the following steps:
(1) select healthy, age 2-3 year, the estrus cycle normal multiparity ewe as treating pregnant sheep, all sheep are all quarantined, prevent epidemic and the process such as dipping, and in feedstuff, supplement greenfeed, vitamin and trace element;
(2) at intramuscular injection Cloprostenol when pregnant sheep is at 7-14d luteal phase, start observation after 30h and oestrus, when observe oestrus after within 12 hours, carry out artificial semenpicking and semen deposition, 24 can be once composite after oestrusing, carry out ultrasound diagnosis after 35 days, determine conceived situation;
(3) when fetal lamb grows to 85-100 days, modus operandi takes out fetal lamb, is placed in the container of high temperature sterilize, cleans with physiological saline solution, removes eyeball, brain, liver, lung, the heart, intestinal stomach function regulating;
(4) with physiological saline solution cleaning, drain away the water, weigh, with the shears of sterilizing, fetal lamb is shredded, put into the refiner homogenate of sterilizing;
(5) add normal saline and fetal lamb fragment carries out homogenate, homogenate adds normal saline dilution, the total amount of normal saline be the 2-4 of fetal lamb quality doubly, homogenate diluent filters through stainless steel filtering net, upper strata 50 order, middle level 100 order, lower floor 200 order;
(6) homogenate filtered solution is placed in the safety glass pallet of sterilizing and places-30 DEG C of refrigerators, freezingly spends the night, or stores;
(7) take out frozen homogenate filtered solution thaw at RT, to complete freeze thawing, proceed in sterile centrifugation container, 3000-5000rpm, centrifugal 8-12 minute, abandons precipitation;
(8) supernatant proceeds to aseptic heatproof container, is placed in 60-80 DEG C of water-bath, and 60-80 DEG C of insulation 20-40 minute, fully kills virus and degeneration foreign protein that fetal lamb may bring into from parent;
(9) put into rapidly-30 DEG C of refrigerators and be cooled to-1-1 DEG C, or frozen;
(10) transfer liquid is to the centrifuge container of sterilizing, 3000-5000rpm, and centrifugal 20-40 minute, abandons precipitation;
(11) supernatant proceeds to sterilization container, uses cross-flow ultrafiltration system, uses different systems, ultrafiltration according to the volume of supernatant;
(12) be divided into two parts after ultrafiltration through membranes, concentrated solution is greater than the foreign protein of 30KD, abandons; Filtered solution is the small molecular protein being less than 30KD; Use cross-flow ultrafiltration system to carry out ultrafiltration and concentration to filtered solution, obtain concentrated solution and filtered solution, carry out 0.22ug filtration sterilization to concentrated solution ,-80 DEG C frozen.
2. the extracting method of micromolecule sheep placental extract according to claim 1, it is characterized in that, the described cross-flow ultrafiltration system of step (11) is Centramate ultrafiltration system and T series membranes bag, 30KD aperture.
3. the extracting method of micromolecule sheep placental extract according to claim 1, it is characterized in that, the described cross-flow ultrafiltration system of step (12) is Centramate ultrafiltration system and T series membranes bag, 30KD aperture, uses 1KD film bag.
4. the extracting method of micromolecule sheep placental extract according to claim 1, it is characterized in that, described micromolecule sheep placental extract color is faint yellow, and the denseer color of concentration is darker, and maximal ultraviolet absorption value is 252nm; PH is 6.7-7.4; SDS-PAGE electrophoretic band is 15KD; Purity 95%; Productive rate is 1g/100g fetal lamb body weight.
5. micromolecule sheep placental extract described in claim 1 is in the application of skin care field, it is characterized in that, the fineness of skin and the lyophilized powder of sophistication is increased for the preparation of wrinkle, described lyophilized powder is made up of the material of following parts by weight: micromolecule sheep placental extract 3-8 part, mannitol 3-7 part, trehalose 3-7 part, hyaluronic acid 0.01-1 part, water 80-90 part.
6. micromolecule sheep placental extract described in claim 1 is in the application of skin care field, it is characterized in that, the fineness of skin and the solution of sophistication is increased for the preparation of wrinkle, described solution is made up of the material of following parts by weight: micromolecule sheep placental extract 4-8 part, mannitol 3-7 part, trehalose 3-7 part, water 80-90 part.
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| CN106148273A (en) * | 2016-07-20 | 2016-11-23 | 陈军 | A kind of preparation method and applications of tire Skins culture cytoactive element |
| CN106821939A (en) * | 2017-03-19 | 2017-06-13 | 湖南海济药业有限公司 | The extracting method of sheep embryo extract and the preparation containing its extract |
| CN109350627A (en) * | 2018-11-28 | 2019-02-19 | 广东冠龙生物科技有限公司 | A kind of freeze-dried sheep placenta extract powder and preparation method thereof |
| CN109939062A (en) * | 2019-03-28 | 2019-06-28 | 成都峻宇生物科技有限公司 | A kind of sheep embryo or sheep placenta extracts, preparation method and the application in cosmetics |
| CN110179736A (en) * | 2019-05-14 | 2019-08-30 | 湖南省锘恩科德生物科技有限公司 | A kind of imitative vernix compositions and the preparation method and application thereof |
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| CN111544367A (en) * | 2020-06-12 | 2020-08-18 | 戴东升 | Sheep embryo extract product and preparation method thereof |
| CN112057409A (en) * | 2020-09-18 | 2020-12-11 | 成都清科生物科技有限公司 | Sheep placenta extracting solution with anti-aging function and preparation method and application thereof |
| CN112315892A (en) * | 2020-12-10 | 2021-02-05 | 西藏天慈生物科技有限公司 | Sheep placenta anti-wrinkle whitening freeze-dried powder and method |
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