CN104382147B - A kind of Herba Portulacae Jerusalem artichoke fermented beverage and preparation method thereof altogether - Google Patents

A kind of Herba Portulacae Jerusalem artichoke fermented beverage and preparation method thereof altogether Download PDF

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CN104382147B
CN104382147B CN201410713542.3A CN201410713542A CN104382147B CN 104382147 B CN104382147 B CN 104382147B CN 201410713542 A CN201410713542 A CN 201410713542A CN 104382147 B CN104382147 B CN 104382147B
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jerusalem artichoke
herba portulacae
juice
fermented beverage
altogether
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CN104382147A (en
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张宏志
马艳弘
唐伯平
黄开红
李亚辉
周春霖
李超峰
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Jiangsu Academy of Agricultural Sciences
Yancheng Teachers University
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Jiangsu Academy of Agricultural Sciences
Yancheng Teachers University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
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  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The invention discloses a kind of Herba Portulacae Jerusalem artichoke fermented beverage and preparation method thereof altogether.The method is with Herba Portulacae and Jerusalem artichoke as raw material, it is carried out respectively, after blanching or the pre-treatment such as steaming and decocting, making beating and sterilizing, mixing, access lactobacillus leaven and carry out band slag fermentation, after fermentation ends, slagging-off is centrifugal, add complex stabilizer, then prepare finished product through pasteurize, hot filling, cooling.This lactic acid bacteria fermentation beverage is dark green, clarify bright, sour and sweet palatability, there is dense lactate fermentation ferment perfume and Herba Portulacae, Jerusalem artichoke delicate fragrance, effective ingredient in Herba Portulacae and Jerusalem artichoke can be fully dissolved out by common fermented beverage prepared by the inventive method, method simple possible, is efficiently applied to the preparation field of pure natural composite fermented beverage.

Description

A kind of Herba Portulacae Jerusalem artichoke fermented beverage and preparation method thereof altogether
Technical field
The invention belongs to food processing field, especially relate to a kind of Herba Portulacae Jerusalem artichoke fermented beverage and preparation method thereof altogether.
Background technology
Herba Portulacae, has another name called horse Amaranthus mangostanus L., Wu Hangcao, longevity greens/mustard green etc., for Portulacaceae annual meat herbaceous plant, has Drought-enduring, impoverishment tolerant, the feature such as fast growth, it is widely distributed in field, vegetable garden and roadside etc., some area starts in recent years It is carried out field plantation.Herba Portulacae contains the multiple nutritional labeling useful to human body and medicinal ingredient, be common in Medical herbs, is also the wild vegetables liked of people, is set to medicine food shared resource by China's Ministry of Public Health.Herba Portulacae contains abundant Omega-3 unsaturated fatty acid, it is possible to atherosclerosis, delay thrombosis, significantly improve cardiovascular disease; It is possibly together with substantial amounts of norepinephrine, it is possible to promote insulin secretion, regulates human body carbohydrate metabolism, has reduction blood glucose Treatment diabetes are had good effect by concentration, the effect of holding glucostasis.Additionally, Herba Portulacae can be used for treating enteritis, dysentery The inflammation diseases such as disease, appendicitis, mastitis, parotitis, pertussis, pulmonary abscess, urinary tract infection, sore swollen toxin, External can control erysipelas and venom, have the title of " natural antibiotics ".Therefore, Herba Portulacae is described as 21 generation by nutritionist Record one of the most promising pollution-free food, as novel food source, increasingly cause the concern of people.
Jerusalem artichoke, popular name Jerusalem artichoke, Rhizoma Zingiberis Recens, for Compositae Helianthus herbaceos perennial, its wide adaptability, impoverishment tolerant, Yield is high, is suitable for planting in beach ground, salt-soda soil.The general per mu yield of Jerusalem artichoke tuber is 2000~4000kg, wherein rich in Inulin (also known as inulin), inulin concentration is the 10%~20% of the wet quality of Jerusalem artichoke, about the 80% of dry mass, is that processing is raw Produce inulin and the preferred raw materials of goods thereof.Inulin is the fruit formed through β (2 → l) glycosidic bond dehydration polymerization by D-Fructose Polysaccharide, in linear chain structure, its end connects 1 glucose residue, and each inulin molecule is containing about 30~50 residue of fructose. Inulin eats polysaccharide as a kind of natural function, is also a kind of water soluble dietary fiber, and inulin can increase bifid in intestinal Bacillus, prevents intestinal infection, promotes the absorption of mineral, controls blood fat, prevents treating constipation and fat, reduces blood glucose;This Outer inulin is largely used to as sugar, fat substitute in low in calories, low sugar, low-fat food, can significantly improve without fat, The mouthfeel of low fat food and texture.Therefore, the exploitation of Girasole synanthrin enjoy the height weight of domestic and international food circle always Depending on.
Herba Portulacae and Jerusalem artichoke are as excellent functional food raw material, and the developmental research to them at present is also nowhere near.Especially Being Herba Portulacae, studies in China is concentrated mainly on the aspects such as medicinal ingredient, pharmacology, traditional Chinese medical science application and potherb resource exploitation utilization, The research of associated drinks is considerably less, and existing document report is also only simple with other raw materials with juice of Herba Portulacae Mixing, adds the fruit and vegetable drink of the adjuvant such as citric acid, sucrose modulation.Therefore, functional higher Herba Portulacae, Jerusalem artichoke serial health product has to be developed, and as combined the fermentation advantage of lactic acid bacteria, prepared fermented beverage has raw material and breast concurrently The two-fold advantage of acid bacteria fermentation food, will have more vast potential for future development, both can meet people to nutrition and health care Demand, abundant soft drink market, considerable economic benefit can be obtained simultaneously.
Summary of the invention
It is an object of the invention to provide and a kind of ferment altogether the beverage of preparation and side through lactic acid bacteria for raw material with Herba Portulacae and Jerusalem artichoke Method, uses the beverage sour and sweet palatability that the method prepares, unique flavor, heat-clearing and toxic substances removing, diuresis of quenching the thirst, is obtaining lactic acid bacteria While can reach again effect of health care, contribute to improving function of intestinal canal, regulate blood glucose, prevent and treat heart disease, diabetes and Cancer etc..
It is an object of the invention to be accomplished by:
A kind of Herba Portulacae Jerusalem artichoke fermented beverage altogether, this beverage is mainly prepared by following steps:
(1) Herba Portulacae blanching in boiling water, mixes making beating, then adds pectinase enzymatic hydrolysis with water after draining, enzyme denaturing, then will Herba Portulacae keeps 10~20min at 80~90 DEG C, and regulation juice of Herba Portulacae pH is to neutrality, sterilizing;
(2) by fresh Jerusalem artichoke tuber steaming and decocting to ripening, clear water rinses, and mixes making beating after draining with water, and Jerusalem artichoke slurry is heated to 80~90 DEG C of holdings 40~60min, sterilizing, obtain jerusalem artichoke juice;
(3) jerusalem artichoke juice mixed by the part by weight of 1:3~1:5 with juice of Herba Portulacae, add mixed juice weight 3-5% sucrose stirring all Even, the inoculum concentration with 4%~8% accesses leaven Lactobacillus plantarum and carries out anaerobic fermentation, filters, slagging-off after fermentation ends, Filtrate is centrifuged, and takes supernatant and obtains fermented juice;
(4) in fermented juice, add complex stabilizer, after mixing, carry out pasteurize;
(5) fermented juice after sterilization carries out rapidly hot filling, cooling.
In step (1), the blanching time is 1~2min.Herba Portulacae blanching drains beats rear mixing according to mass ratio 1:1~1:3 with water Slurry.The addition of pectase is that juice of Herba Portulacae weighs 0.05%.Enzymolysis time is 2~3h.
By steaming and decocting under 90 DEG C of water bath condition of fresh Jerusalem artichoke tuber to ripening in step (2), clear water rinse, drain after and water by Pull an oar after mass ratio 1:3~1:5 mixing.
Step (3) is seasoned adding the Mel that can also add 2% while sucrose.Described anaerobic fermentation bar Part is preferably anaerobic fermentation 14~18h at 37~39 DEG C.
Complex stabilizer described in step (4) is xanthan gum 0.005-0.015%, guar gum 0.006-0.012%, Food additive anti-acid CMC (carboxylic first class sodium cellulosate) 0.004-0.008%, preferably xanthan gum 0.010%, Guar Locust beam gum 0.006%, anti-acid CMC 0.008%.Pasteurizing temperature is 85 DEG C, and the time is 30min.
The preparation method of above-mentioned Herba Portulacae Jerusalem artichoke fermented beverage altogether, the method comprises the following steps:
(1) Herba Portulacae blanching in boiling water, mixes making beating, then adds pectinase enzymatic hydrolysis with water after draining, enzyme denaturing, then will Herba Portulacae keeps 10~20min at 80~90 DEG C, and regulation juice of Herba Portulacae pH is to neutrality, sterilizing;
(2) by fresh Jerusalem artichoke tuber steaming and decocting to ripening, clear water rinses, and mixes making beating after draining with water, and Jerusalem artichoke slurry is heated to 80~90 DEG C of holdings 40~60min, sterilizing, obtain jerusalem artichoke juice;
(3) jerusalem artichoke juice mixed by the part by weight of 1:3~1:5 with juice of Herba Portulacae, add mixed juice weight 3-5% sucrose stirring all Even, the inoculum concentration with 4%~8% accesses leaven Lactobacillus plantarum and carries out anaerobic fermentation, filters, slagging-off after fermentation ends, Filtrate is centrifuged, and takes supernatant and obtains fermented juice;
(4) in fermented juice, add complex stabilizer, after mixing, carry out pasteurize;
(5) fermented juice after sterilization carries out rapidly hot filling, cooling.
The preparation method of above-mentioned Herba Portulacae Jerusalem artichoke altogether fermented beverage, mainly with juice of Herba Portulacae and jerusalem artichoke juice as primary raw material, adds With sucrose and Mel, accessing lactic acid bacteria and carry out common fermentation, concrete technical step includes:
(1) select the Herba Portulacae that root is cleaned, blanching 1~2min in the boiling water of 3 times of quality, drain afterwards and water is by quality Making beating after mixing than 1:1~1:3, then add pectase enzymolysis 2~3h under 45 DEG C of water-baths of 0.05%, then 90 DEG C of water-bath 30s Enzyme denaturing.Herba Portulacae slurry is heated to 80~90 DEG C and keeps 10~20min, regulates juice of Herba Portulacae pH extremely by the NaOH solution of 0.1M Neutrality, in 121 DEG C of sterilizing 15min, stand-by.
(2) being cleaned up by fresh Jerusalem artichoke tuber, under 90 DEG C of water bath condition, steaming and decocting 40min rinses to ripening, clear water, drip With water making beating after 1:3~1:5 mixes in mass ratio after dry.Jerusalem artichoke slurry is heated to 80~90 DEG C and keeps 40~60min, goes out in 121 DEG C Bacterium 15min, obtains jerusalem artichoke juice, stand-by.
(3) jerusalem artichoke juice mixed in the ratio of 1:3~1:5 with juice of Herba Portulacae, add 5% sucrose and 2% Mel stirs, Inoculum concentration with 4%~8% accesses leaven Lactobacillus plantarum, and anaerobic fermentation 14~18h at 37~39 DEG C, after fermentation ends With 8 layers of filtered through gauze slagging-off.Filtrate centrifugal treating after slagging-off, 4500r/min is centrifuged 25min, takes supernatant and obtains clarification thoroughly Bright fermented juice.
(4) clear fermented juice adds xanthan gum 0.010%, guar gum 0.006% and anti-acid CMC 0.008%, Carrying out pasteurize after mixing, sterilization temperature is 85 DEG C, and the time is 30min.
(5) fermented juice after sterilization carries out rapidly hot filling, gets product after cooling.
Jerusalem artichoke pre-treatment the most all uses the chemical substances such as interpolation sodium sulfite, ascorbic acid and citric acid to prevent enzymatic Brown stain, the present invention uses Jerusalem artichoke steaming and decocting to the pretreatment mode of ripening to prevent and treat brown stain, had both reached good prevent-browning effect Really, add again the dissolution of inulin in Jerusalem artichoke, obtained unforeseeable effect.
The leaven Lactobacillus plantarum (Lactobacillus plantarum 70810) used in the present invention is isolatable from Pickles, On July 10th, 2011 at the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number CGMCC No.2843。
Polyoses content=total sugar content-content of reducing sugar in beverage of the present invention
Being respectively adopted phend-sulphuric acid and 3 in the present invention, it is total that 5-dinitrosalicylic acid system (DNS method) measures polysaccharide in beverage Sugar and the content of reducing sugar.
In the present invention, Herba Portulacae Jerusalem artichoke omega-3 unsaturated fatty acid assay method is as follows:
(1) esterification processes: accurately weighs Herba Portulacae jerusalem artichoke beverage 0.3ml g and is placed in 10mL scale sample bottle, successively Add 5mL normal hexane, 3mL0.05% potassium hydroxide-methanol solution, be placed in 60 DEG C of water-baths reaction 30min, with steaming Distilled water is settled to 10mL, shaken well, and 10000r/min is centrifuged 5min, takes supernatant and carries out gas chromatographic analysis.
(2) chromatography of gases analysis: Agilen 6890 chromatograph, HP-5 quartz capillary column (30m × 0.32mm × 0.25 μm), carrier gas is N2, flow is 1mL/min, and detector is FID, and sample size is 1 μ L, Split ratio is that 40:1, injection port and detector temperature are respectively 260 DEG C and 280 DEG C, temperature programming: initial temperature 150 DEG C guarantor Hold 5min, rise to 250 DEG C with 10 DEG C/min, keep 10min.
Below by way of concrete test, character of the present invention and major parameter are illustrated:
1, in pre-treatment, inulin dissolution processes time and the screening of temperature
Weigh fresh Jerusalem artichoke that 10g pretreatment (preprocess method is with embodiment 1) crosses in 250mL conical flask, selected material Liquor ratio is 1:5, research leaching temperature and the time impact on inulin dissolution rate.
Table 1 inulin dissolution experiment screening result
The condition that Girasole synanthrin dissolution processes is: solid-liquid ratio 1:5, leaching temperature is 80~90 DEG C, and dissolution time is 40~60min.In this condition and range, inulin dissolution rate is higher.
2, the screening of lactobacillus beverage fermentation condition, remaining step is with embodiment 1.
Table 2 fermentation condition the selection result
The preferred fermentation condition of Herba Portulacae Jerusalem artichoke lactobacillus beverage is: inoculum concentration 4~8%, fermentation temperature 37~39 DEG C, sends out The ferment time 14~18h.In this condition and range, lactic acid production is notable.
3, the screening of lactobacillus beverage stabilizer formula:
Characteristic according to different stabilizers and pre-stage test result, select respectively xanthan gum, guar gum, sodium alginate, 5 kinds of monomer stabilization agent such as anti-acid CMC and agar, carry out single factor experiment with centrifugation rate for index, remaining step Rapid with embodiment 1.Experiment shows that single stable agent is extremely difficult to preferable effect, need to consider to use complex stabilizer, therefore The preferable xanthan gum of stability, guar gum and anti-acid CMC is selected to carry out orthogonal test, to determine that optimum proportioning is used Amount.
Table 3 stabilizer formula screening Orthogonal experiment results
From table 3 range analysis, xanthan gum affects maximum to the stability of beverage, next to that anti-acid CMC, What action effect was minimum is guar gum.Shown that the best stabilizer is combined as A2B1C3 by orthogonal test, it may be assumed that xanthan gum 0.010%, guar gum 0.006%, anti-acid CMC0.008%.Empirical tests, this parameter combination product centrifugation rate It is 0.31%.
4, the Herba Portulacae Jerusalem artichoke lactobacillus beverage impact on alcoholic liver injury protective effect
1. the foundation of acetaldehyde induction hepatic stellate cell (HSC) multiplicative model
Use the method adding different acetaldehyde concentration HSC stimulated effect 24h, set up the isolated cells of cultured rat hepatic stellate cells Model.Repeatedly selecting 1.25 times after trial test to be incremented by, with 25umol/L as initial concentration, concentration of taking the logarithm is to propagation effect The growth curve of rule, its irritaiting concentration (EC should be presented50) it is 84umol/L.Therefore selecting acetaldehyde concentration is that 84umol/L makees Concentration for the cell model of the in vitro alcoholic fibrosis of experimental rat.
2. the lactobacillus beverage impact on acetaldehyde induction HSC proliferation
Cell number is adjusted to 5 × 104Individual/mL, is inoculated in 96 well culture plates, cultivates 24h, and often the multiple hole of group 6, adds Enter acetaldehyde that concentration is 84umol/L and the Herba Portulacae Jerusalem artichoke lactobacillus beverage of different extension rates, separately set model group and right According to group.Tetramethyl azo azoles salt (MTT) method is used to measure the rate of increase of each group of cell after continuing to cultivate 24h.
As it is shown in figure 1, Cell culture invitro research shows that Herba Portulacae Jerusalem artichoke lactobacillus beverage can effectively suppress acetaldehyde to induce HSC proliferation.This beverage rejection ability under the concentration of extension rate 10 times is more notable, and its maximal percentage inhibition is 63%.
Advantages of the present invention and having the active effect that
1) the pure juice of Herba Portulacae is micro-sour, more puckery, and medium-height grass flavour of a drug are dense, directly prepares beverage taste not good enough, joins with jerusalem artichoke juice 5, carry out lactate fermentation, give abundant flavour and fragrance, mouthfeel can be effectively improved.
2) present invention is during preparing jerusalem artichoke juice, and by Jerusalem artichoke steaming and decocting to curing state, not only prevent-browning effect is notable, protects Demonstrate,prove jerusalem artichoke juice Color Quality;Also contribute to dissolving and the dissolution of inulin in Jerusalem artichoke cell simultaneously, significantly increase jerusalem artichoke juice In inulin concentration, will be a step the most necessary in pre-treatment.
3) general beverage made of fruits or vegetables fermentation is all first removed filtering residue and is fermented, i.e. fermentation of clear juice.The present invention uses the fermentation of band slag Technique, by lactic acid bacteria metabolism and conversion, farthest utilizes the nutritional and officinal compositions in fermentation substrate, compares clear Juice ferments, and lactate fermentation ferment is fragrant and Herba Portulacae, Jerusalem artichoke delicate fragrance are the denseest.
4) fermentation beverage made of fruits or vegetables mainly uses Yoghourt Production the bacterial strain such as streptococcus thermophilus and Bulgaria got through domestication at present Lactobacillus, strain growth is slow, and ferment effect is the best, and taste is inharmonious.The present invention selects lactobacillus leaven especially For being isolatable from the Lactobacillus plantarum of Pickles, making the beverage taste harmony and natural prepared, viable count of lactobacillus is high and the later stage survives Rate is high.Because from being all the Pickles of plant source, Lactobacillus plantarum will have incomparable on fermentation Herba Portulacae and Jerusalem artichoke Superiority.
5) in the present invention preparation Herba Portulacae Jerusalem artichoke lactobacillus beverage merged Polysaccharide from Portulaca oleracea, the prebiotic function of inulin and The probiotic action of lactic acid bacteria, and, alcoholic hepatic injury is had significant protective effect, is a kind of novel functional Beverage.
6) selecting the Herba Portulacae of integration of edible and medicinal herbs and Jerusalem artichoke as raw material in the present invention, the lactobacillus beverage of preparation is rich in nor-kidney The various active compositions such as upper parathyrine, omega-3 unsaturated fatty acid, vitamin, Polysaccharide from Portulaca oleracea and inulin, have heat clearing away solution Poison, dehumidifying detumescence, effects out of the ordinary such as diuresis of quenching the thirst, it is also possible to promote human body carbohydrate metabolism, keep glucostasis, improve intestinal Road flora, strengthen function of intestinal canal, significantly reduce cholesterol, triglyceride levels, to diabetes, coronary heart disease, hypertension, Cardiopathic preventing and treating has preferable effect.
Accompanying drawing explanation
Fig. 1 lactobacillus beverage of the present invention affects figure to acetaldehyde induction HSC proliferation.
Detailed description of the invention
For the apparent understanding present invention, below in conjunction with example, the present invention is described further.But embodiment is concrete Details is only used for explaining the present invention, limits the present invention never in any form.
Embodiment 1:
Select root clean Herba Portulacae 300g, blanching 1min in the boiling water of 3 times of quality, drain after and water in mass ratio Pull an oar after 1:2 mixing, add the pectase HC (LALLZYME HC, LALLEMAND company of France) 45 DEG C of 0.05% Enzymolysis 2h under water bath condition, enzymolysis terminates to be placed on 90 DEG C of water-bath 30s inactivations.Herba Portulacae slurry is heated to 85 DEG C and keeps 15min, Juice of Herba Portulacae pH is regulated the most neutral by the NaOH solution of 0.1M, in 121 DEG C of sterilizing 15min, stand-by.Take and clean up Fresh Jerusalem artichoke tuber 100g, under 90 DEG C of water bath condition, steaming and decocting 45min is to ripening.Clear water rinses, and drains afterwards and water is by quality Pull an oar after mixing than 1:3.Jerusalem artichoke slurry is heated to 80 DEG C and keeps 60min, in 121 DEG C of sterilizing 15min, obtains jerusalem artichoke juice, treats With.Take jerusalem artichoke juice to mix in the ratio of 1:3 with juice of Herba Portulacae, add mixed juice and weigh 5% sucrose and 2% Mel stirs, Inoculum concentration with 4% accesses leaven Lactobacillus plantarum, and anaerobic fermentation 18h at 37 DEG C, by 8 layers of gauze mistake after fermentation ends Filter slag, and do centrifugal treating (4500r/min is centrifuged 25min).Take supernatant and obtain clear fermented juice, add clear Clear bright fermented juice weigh 0.010% xanthan gum, the guar gum of 0.006%, the anti-acid CMC of 0.008%, mixing, enter Row pasteurize (temperature is 85 DEG C, and the time is 30min) carries out the most rapidly hot filling, and cooling gets product.
Jerusalem artichoke Herba Portulacae prepared by this enforcement special case fermentative lactobacillus beverage altogether is dark green, clarifies bright, sour and sweet palatability, Lactate fermentation ferment is fragrant and Herba Portulacae, Jerusalem artichoke delicate fragrance are dense.Preserve respectively through 4 DEG C and 37 DEG C and within 3 months, be showed no layering, almost Not precipitating generation, taste flavor all keeps preferable, and main physics and chemistry and nutrient content vary less, the results detailed in Table 4.
The main physics and chemistry of beverage and nutritive index in table 4 embodiment 1
Embodiment 2:
Select root clean Herba Portulacae 300g, blanching 1.5min in the boiling water of 3 times of quality, drain after and water in mass ratio Pull an oar after 1:3 mixing, add the pectase HC (LALLZYME HC, LALLEMAND company of France) 45 DEG C of 0.05% Enzymolysis 2.5h under water bath condition, enzymolysis terminates to be placed on 90 DEG C of water-bath 30s inactivations.Herba Portulacae slurry is heated to 90 DEG C of holdings 10min, regulates juice of Herba Portulacae pH by the NaOH solution of 0.1M the most neutral, in 121 DEG C of sterilizing 15min, stand-by.Take clear The fresh Jerusalem artichoke tuber 100g of wash clean, under 90 DEG C of water bath condition, steaming and decocting 45min is to ripening.Clear water rinse, drain after with Pull an oar after water 1:4 in mass ratio mixing.Jerusalem artichoke slurry is heated to 85 DEG C and keeps 50min, in 121 DEG C of sterilizing 15min, obtains chrysanthemum Taro juice, stand-by.Jerusalem artichoke juice is mixed in the ratio of 1:4 with juice of Herba Portulacae, adds mixed juice and weigh 3% sucrose and 2% Mel stirs Mixing uniformly, the inoculum concentration with 6% accesses leaven Lactobacillus plantarum, and anaerobic fermentation 16h at 38 DEG C, with 8 after fermentation ends Layer filtered through gauze slagging-off, and do centrifugal treating (4500r/min is centrifuged 25min).Take supernatant and obtain clear fermented juice, Add clear fermented juice weigh 0.010% xanthan gum, the guar gum of 0.006%, the anti-acid CMC of 0.008%, mixed Even, carry out pasteurize (temperature is 85 DEG C, and the time is 30min) and carry out the most rapidly hot filling, cooling gets product.
Jerusalem artichoke Herba Portulacae prepared by this enforcement special case fermentative lactobacillus beverage altogether is dark green, clarifies bright, sour and sweet palatability, Lactate fermentation ferment is fragrant and Herba Portulacae, Jerusalem artichoke delicate fragrance are dense.Within 3 months, it is showed no layering through 4 DEG C and 37 DEG C storages, almost without Precipitation produces, and taste flavor all keeps preferable, and main physics and chemistry and nutrient content vary less, the results detailed in Table 5.
The main physics and chemistry of beverage and nutritive index in table 5 embodiment 2
Embodiment 3:
Select root clean Herba Portulacae 300g, blanching 2min in the boiling water of 3 times of quality, drain after and water in mass ratio Pull an oar after 1:1 mixing, add the pectase HC (LALLZYME HC, LALLEMAND company of France) 45 DEG C of 0.05% Enzymolysis 3h under water bath condition, enzymolysis terminates to be placed on 90 DEG C of water-bath 30s inactivations.Herba Portulacae slurry is heated to 80 DEG C and keeps 20min, Juice of Herba Portulacae pH is regulated the most neutral by the NaOH solution of 0.1M, in 121 DEG C of sterilizing 15min, stand-by.Take and clean up Fresh Jerusalem artichoke tuber 100g, under 90 DEG C of water bath condition, steaming and decocting 45min is to ripening.Clear water rinses, and drains afterwards and water is by quality Pull an oar after mixing than 1:5.Jerusalem artichoke slurry is heated to 90 DEG C and keeps 40min, in 121 DEG C of sterilizing 15min, obtains jerusalem artichoke juice, treats With.Jerusalem artichoke juice is mixed in the ratio of 1:5 with juice of Herba Portulacae, adds mixed juice and weigh 5% sucrose and 2% Mel stirs, Inoculum concentration with 8% accesses leaven Lactobacillus plantarum, and anaerobic fermentation 14h at 39 DEG C, by 8 layers of gauze mistake after fermentation ends Filter slag, and do centrifugal treating (4500r/min is centrifuged 25min).Take supernatant and obtain clear fermented juice, add clear Clear bright fermented juice weigh 0.010% xanthan gum, the guar gum of 0.006%, the anti-acid CMC of 0.008%, mixing, enter Row pasteurize (temperature is 85 DEG C, and the time is 30min) carries out the most rapidly hot filling, and cooling gets product.
Jerusalem artichoke Herba Portulacae prepared by this enforcement special case fermentative lactobacillus beverage altogether is dark green, clarifies bright, sour and sweet palatability, Lactate fermentation ferment is fragrant and Herba Portulacae, Jerusalem artichoke delicate fragrance are dense.Within 3 months, it is showed no layering through 4 DEG C and 37 DEG C storages, almost without Precipitation produces, and taste flavor all keeps preferable, and main physics and chemistry and nutrient content vary less, the results detailed in Table 6.
The main physics and chemistry of beverage and nutritive index in table 6 embodiment 3

Claims (9)

1. a Herba Portulacae Jerusalem artichoke fermented beverage altogether, it is characterised in that this beverage is mainly prepared by following steps:
(1) Herba Portulacae blanching in boiling water, mixes making beating, then adds pectinase enzymatic hydrolysis with water after draining, enzyme denaturing, then will Herba Portulacae keeps 10~20min at 80~90 DEG C, and regulation juice of Herba Portulacae pH is to neutrality, sterilizing;
(2) by 90 DEG C of water bath condition steaming and decoctings of fresh Jerusalem artichoke tuber to ripening, clear water rinse, drain after and water in mass ratio 1:3~1:5 mixes making beating, and Jerusalem artichoke slurry is heated to 80~90 DEG C of holdings 40~60min, sterilizing, obtains jerusalem artichoke juice;
(3) jerusalem artichoke juice mixed by the part by weight of 1:3~1:5 with juice of Herba Portulacae, add 3-5% sucrose and stir, with The inoculum concentration of 4%~8% accesses leaven Lactobacillus plantarum and carries out anaerobic fermentation, filters after fermentation ends, slagging-off, filtrate from The heart, takes supernatant and obtains fermented juice;
(4) in fermented juice, add complex stabilizer, after mixing, carry out pasteurize;
(5) fermented juice after sterilization carries out rapidly hot filling, cooling.
Herba Portulacae Jerusalem artichoke the most according to claim 1 fermented beverage altogether, it is characterised in that blanching time in step (1) It is 1~2min.
Herba Portulacae Jerusalem artichoke the most according to claim 1 fermented beverage altogether, it is characterised in that Herba Portulacae drift in step (1) Scald and mix making beating with water according to mass ratio 1:1~1:3 after draining.
Herba Portulacae Jerusalem artichoke the most according to claim 1 fermented beverage altogether, it is characterised in that pectase in step (1) Addition is 0.05%.
Herba Portulacae Jerusalem artichoke the most according to claim 1 fermented beverage altogether, it is characterised in that enzymolysis time in step (1) It is 2~3h.
Herba Portulacae Jerusalem artichoke the most according to claim 1 fermented beverage altogether, it is characterised in that answering described in step (4) Conjunction stabilizer is xanthan gum 0.005-0.015%, guar gum 0.006-0.012%, CMC 0.004-0.008%.
Herba Portulacae Jerusalem artichoke the most according to claim 6 fermented beverage altogether, it is characterised in that answering described in step (4) Closing stabilizer is xanthan gum 0.010%, guar gum 0.006%, anti-acid CMC 0.008%.
Herba Portulacae Jerusalem artichoke the most according to claim 1 fermented beverage altogether, it is characterised in that detesting described in step (3) Aerobe fermentation condition is anaerobic fermentation 14~18h at 37~39 DEG C.
Herba Portulacae Jerusalem artichoke the most according to claim 1 fermented beverage altogether, it is characterised in that pasteurize in step (4) Temperature is 85 DEG C, and the time is 30min.
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CN105147564B (en) * 2015-09-30 2018-08-24 北京工商大学 A kind of purslane proferment pulp cosmetic and the preparation method and application thereof
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102757875A (en) * 2012-07-15 2012-10-31 马宁 Fermented type purslane red wine and application
CN102860548A (en) * 2012-09-17 2013-01-09 江苏大学 Jerusalem artichoke juice and active lactobacillus beverage and preparation method thereof
CN102986893A (en) * 2012-12-03 2013-03-27 山东建筑大学 Jerusalem artichoke-soybean fermentation beverage and preparation method thereof
CN103039927A (en) * 2012-12-28 2013-04-17 徐州绿之野生物食品有限公司 Biologically fermented black purslane food and preparation method thereof
CN103704837A (en) * 2013-12-09 2014-04-09 安徽圣芝堂生物科技有限公司 Beverage with function of invigorating stomach

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102757875A (en) * 2012-07-15 2012-10-31 马宁 Fermented type purslane red wine and application
CN102860548A (en) * 2012-09-17 2013-01-09 江苏大学 Jerusalem artichoke juice and active lactobacillus beverage and preparation method thereof
CN102986893A (en) * 2012-12-03 2013-03-27 山东建筑大学 Jerusalem artichoke-soybean fermentation beverage and preparation method thereof
CN103039927A (en) * 2012-12-28 2013-04-17 徐州绿之野生物食品有限公司 Biologically fermented black purslane food and preparation method thereof
CN103704837A (en) * 2013-12-09 2014-04-09 安徽圣芝堂生物科技有限公司 Beverage with function of invigorating stomach

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
马齿苋酸奶的工艺研究;霍乃蕊等;《食品工程》;20101230(第4期);第21页右栏第2段,第22页第1.3~1.4、2.1节 *

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