CN104374850B - A kind of diaphragm type sampling device - Google Patents
A kind of diaphragm type sampling device Download PDFInfo
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- CN104374850B CN104374850B CN201410573917.0A CN201410573917A CN104374850B CN 104374850 B CN104374850 B CN 104374850B CN 201410573917 A CN201410573917 A CN 201410573917A CN 104374850 B CN104374850 B CN 104374850B
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- adapter
- diaphragm
- shunt
- sampling device
- upper adapter
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- 238000005070 sampling Methods 0.000 title claims abstract description 19
- 230000013011 mating Effects 0.000 claims abstract description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000007791 liquid phase Substances 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 22
- 238000009792 diffusion process Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000004811 liquid chromatography Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- 241000168254 Siro Species 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Abstract
The invention belongs to chromatographic analyzer of liquid phase device field, be specifically related to a kind of diaphragm type sampling device, it comprises the upper adapter (1) that inside has cavity, and the lower adapter (2) that matches of upper adapter and the diaphragm (5) that is placed between adapter (1) and lower adapter (2); Also comprise in the internal cavities being arranged at adapter (1) and be provided with shunt (3), the shape of described shunt (3) and the mating shapes of upper adapter inner cavity, and between described shunt (3) and upper adapter inwall, have the gap of distance≤2mm, in gap, be provided with at least one protruding connection shunt and upper adapter inwall.This device is changed with radial by the axis of elute soln, reduces the divergence factor of wash-out, increases the load sample area of diaphragm.
Description
Technical field
The invention belongs to chromatographic analyzer of liquid phase device field, be specifically related to a kind of diaphragm type sampling device.
Background technology
In liquid-phase chromatographic analysis, sample solution is normally injected quantitative loop by input mode, and by transfer valve access liquid chromatographic system, therefore sample must be in solution state ability sample introduction.But a lot of material easily decomposes in the solution, adsorb and conversion etc., be unfavorable for long-term placement and preservation, therefore need a kind ofly to be conducive to the liquid chromatography sample injection method that placement stablized by sample.
There is a kind of dry blood cake paper disk method (driedbloodspot with liquid chromatography coupling of bibliographical information, DBS) technology, its process is that sample (such as blood, biological fluid etc.) is dropped in a kind of circular paper, sample is volatilized naturally or dries up, owing to not having liquid, what therefore sample can be more stable sticks on the described scraps of paper.During use, the scraps of paper are placed in contiguity system, contiguity system is connected in the liquid chromatography stream before chromatographic column by transfer valve or mechanical system, elute soln enters in described contiguity system by the pipeline that is connected, target components from vertical direction dissolving also the wash-out scraps of paper, thus target components enters the chromatographic column in downstream from wash-out the scraps of paper, last separated detection.Described contiguity system can be understood as: its inside has the upper adapter of cavity, and the lower adapter that upper adapter matches and the scraps of paper be placed between adapter and lower adapter, the top of upper adapter is provided with mobile phase flow ipe, and the lower end of lower adapter is provided with mobile phase outflow conduit.
There is such shortcoming in above-mentioned contiguity system: because elute soln is in described contiguity system, the scraps of paper are flowed therethrough from the direction with scraps of paper plane orthogonal, and the bore of elute soln introducing port is much smaller than the diameter of the scraps of paper, therefore from scraps of paper centre distance more away from position, elute soln flowing velocity is less, thus the sample that the decentering that the scraps of paper distributes is far away, target components needs the longer time to be eluted, the sample that decentering is nearer, target components needs the shorter time to be eluted, and causes the serious diffusion of object eluting peak.Current solution reduces diaphragm area, reduces elute soln to the path of edge-diffusion, allow comparatively near with compared with far-end sample eluting in the time range that can tolerate, but scraps of paper area is too little, can limit the volume containing the sample of sample.Therefore improve existing paper disk method sample introduction, provide a kind of load sample area large, and diffusion is little, the diaphragm type sampling device that elution time is few is significant.
Summary of the invention
For deficiency of the prior art, the present invention aims to provide a kind of diaphragm type sampling device, this device is changed with radial by the axis of elute soln, elute soln is forced to flow to central authorities from diaphragm periphery, the object be loaded on diaphragm can be concentrated flow out from central authorities, reduce the diffusion of wash-out, thus the area of load sample diaphragm can be increased.
For achieving the above object, technical scheme of the present invention is:
A kind of diaphragm type sampling device, comprises the upper adapter that inside has cavity, and the lower adapter that matches of upper adapter and the diaphragm that is placed between adapter and lower adapter; Also comprise the shunt be arranged in the internal cavities of adapter, the shape of described shunt and the mating shapes of upper adapter inner cavity, and between described shunt and upper adapter inwall, have the gap of distance≤2mm, in gap, be provided with at least one protruding connection shunt and upper adapter inwall.
Preferred version: the lower end of described shunt is protruded from the inner chamber of upper adapter, projection and diaphragm are fitted.
Described shunt is preferably right cylinder, and right cylinder periphery is preferably provided with at least two projections, and shunt is fastened in the inner chamber of adapter by protruding.
Above-mentioned diaphragm type sampling device, preferably also comprises the diaphragm clamper that two is through; Described diaphragm is placed in diaphragm clamper inner chamber, and described diaphragm clamper is placed between adapter and lower adapter.Under the extruding of external force, diaphragm clamper can make adapter and lower adapter better be tightly connected.
Described upper adapter is preferably coaxial with shunt, and gap between described shunt and upper adapter inwall is even.
Described diaphragm is the porous membrane that can pass through solution, and material is preferably selected from fiber, glass fibre, teflon, polyethersulfone or nylon.
The present invention will be further explained below:
Principle of the present invention is: as depicted in figs. 1 and 2, a kind of large area diaphragm type sampling device of the present invention, and during use, adapter and lower end adapter in compression, diaphragm is adapted the compacting of device outshot.After access chromatographic system, when elute soln enters upper adapter from upper end edge pipeline axial, after running into shunt, elute soln flows out along the gap between upper adapter and shunt, realizes flowing to the change from axially to radial direction; Due to the lower end protruding portion of shunt, be adjacent between diaphragm and shunt, lower end adapter, therefore elute soln is along the outer rim of diaphragm radially to center flow, thus solable matter in the sample on diaphragm is rinsed, and flows to downstream chromatographic system by lower end adapter pipeline.Therefore no matter sample is placed on diaphragm which place, all distally can be promoted to center from diaphragm footpath by elute soln; And the outer rim of diaphragm first contacts elute soln, therefore naturally permeating direction is also move from outer rim to center, thus reduces the diffusion of elute soln wash-out object.
As shown in Figure 3, if do not use shunt, then elute soln major part therefrom runs through in the past, partially liq can on film, from centre, outside edge permeates gradually, dissolved by object on sample point gradually, and rely on the exchange of solution to be eluted, therefore sample point decentering is far away, exchange velocity is slower, and elution time is also longer.
After using shunt, sample spot is on diaphragm, elute soln from upper cover pipeline out, shunt forces and flows out to edge from elute soln center, diaphragm is then from edge to center flow, as shown in Figure 4, no matter sample point is in which position, and soluble substance all will be brought into center by elute soln.And more early contact elute soln the closer to the sample of diaphragm outer.
Shunt lower end should protrude from end adapter, forms outshot, in order to be close to diaphragm.
Shunt is coaxial with upper end adapter, and gap is even.
Diaphragm should with the projection of shunt and lower end adapter compact siro spinning technology, avoid elute soln from film contact Clearance Flow, improve membrane surface and inner flow uniformity.
Described diaphragm clamper is placed in the middle of upper and lower side adaptation end, can possess clamping and sealing function.
Accompanying drawing explanation
Fig. 1 is the decomposition texture schematic diagram of diaphragm type sampling device in the embodiment of the present invention;
Fig. 2 is the structural representation after the sections fit of diaphragm type sampling device in the embodiment of the present invention;
Fig. 3 is when not using shunt, and elute soln flows to schematic diagram on diaphragm;
Fig. 4 uses after shunt, and elute soln flows to schematic diagram on diaphragm;
Wherein, 1 is upper adapter, and 2 is lower adapters, and 3 is shunts, and 4 is protruding, and 5 is diaphragms, and 6 is diaphragm clampers, and 7 is liquid inflow pipes, and 8 is liquid effusers.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described further.
As shown in Figure 1-2, a kind of diaphragm type sampling device, comprise the upper adapter 1 that inside has cavity, and the lower adapter 2 that upper adapter matches and the diaphragm 5 be placed between adapter 1 and lower adapter 2, shunt 3 is provided with in the internal cavities of upper adapter 1, have the gap of distance≤2mm between shunt 3 and upper adapter inwall, and the lower end of shunt 3 is protruded from the inner chamber of upper adapter 1, projection and diaphragm 5 are fitted.
Described shunt 3 is preferably right cylinder, and right cylinder periphery is preferably provided with at least two projections 4, and shunt 3 is fastened in the inner chamber of adapter 1 by protruding 4.
A kind of diaphragm type sampling device also comprises the through diaphragm clamper 6 in two; Diaphragm 5 is placed in diaphragm clamper 6 inner chamber, and diaphragm clamper 6 is placed between adapter 1 and lower adapter 2, and adapter 1 and lower adapter 2 are tightly connected.
Claims (6)
1. a diaphragm type sampling device, comprise the upper adapter (1) that inside has cavity, and the lower adapter (2) that upper adapter matches and the diaphragm (5) be placed between adapter (1) and lower adapter (2), it is characterized in that, also comprise the shunt (3) in the internal cavities being arranged at adapter (1), the shape of described shunt (3) and the mating shapes of upper adapter inner cavity, and between described shunt (3) and upper adapter inwall, have the gap of distance≤2mm, at least one protruding connection shunt and upper adapter inwall is provided with in gap.
2. diaphragm type sampling device according to claim 1, it is characterized in that, described shunt (3) is right cylinder, and right cylinder periphery is provided with at least two projections (4), and described shunt (3) is fastened in the inner chamber of adapter (1) by protruding (4).
3. diaphragm type sampling device according to claim 1, it is characterized in that, the lower end of described shunt (3) is protruded from the inner chamber of upper adapter (1), and projection and diaphragm (5) are fitted.
4. according to the described diaphragm type sampling device of one of claim 1-3, it is characterized in that, this device also comprises the through diaphragm clamper (6) in two; Described diaphragm (5) is placed in diaphragm clamper (6) inner chamber, and described diaphragm clamper (6) is placed between adapter (1) and lower adapter (2).
5. according to the described diaphragm type sampling device of one of claim 1-3, it is characterized in that, described upper adapter (1) is coaxial with shunt (3), and between described shunt (3) and upper adapter inwall, gap is even.
6. according to the described diaphragm type sampling device of one of claim 1-3, it is characterized in that, described diaphragm (5) is for can pass through the porous membrane of solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573917.0A CN104374850B (en) | 2014-10-24 | 2014-10-24 | A kind of diaphragm type sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573917.0A CN104374850B (en) | 2014-10-24 | 2014-10-24 | A kind of diaphragm type sampling device |
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| Publication Number | Publication Date |
|---|---|
| CN104374850A CN104374850A (en) | 2015-02-25 |
| CN104374850B true CN104374850B (en) | 2016-01-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410573917.0A Active CN104374850B (en) | 2014-10-24 | 2014-10-24 | A kind of diaphragm type sampling device |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389726A (en) * | 2001-06-06 | 2003-01-08 | 中国科学院大连化学物理研究所 | Column head and column tail structure for liquid phase chromatographic column with zero dead volume and fine inner diameter |
| CN102741697A (en) * | 2009-12-02 | 2012-10-17 | 沃特曼国际有限公司 | Methods and systems for processing samples on porous substrates |
| CN202811312U (en) * | 2011-05-09 | 2013-03-20 | 安捷伦科技有限公司 | Fluid pump, diverter and fluid separator |
| CN204142690U (en) * | 2014-10-24 | 2015-02-04 | 王峰 | A kind of diaphragm type sampling device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005060303A1 (en) * | 2005-12-16 | 2007-06-28 | Gerstel Systemtechnik Gmbh & Co.Kg | Method of preparing samples for an analyzer and sample preparation station therefor |
| CH703256A1 (en) * | 2010-06-04 | 2011-12-15 | Werner Doebelin | Method and apparatus for automatic and direct analysis of dried blood spots samples by LC-MS system. |
| JP6182547B2 (en) * | 2012-02-14 | 2017-08-16 | ウオーターズ・テクノロジーズ・コーポレイシヨン | Dry sample carrier with dissolvable sample collection area |
| WO2013148071A1 (en) * | 2012-03-30 | 2013-10-03 | Waters Technologies Corporation | Sample carrier for dried biological samples |
-
2014
- 2014-10-24 CN CN201410573917.0A patent/CN104374850B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389726A (en) * | 2001-06-06 | 2003-01-08 | 中国科学院大连化学物理研究所 | Column head and column tail structure for liquid phase chromatographic column with zero dead volume and fine inner diameter |
| CN102741697A (en) * | 2009-12-02 | 2012-10-17 | 沃特曼国际有限公司 | Methods and systems for processing samples on porous substrates |
| CN202811312U (en) * | 2011-05-09 | 2013-03-20 | 安捷伦科技有限公司 | Fluid pump, diverter and fluid separator |
| CN204142690U (en) * | 2014-10-24 | 2015-02-04 | 王峰 | A kind of diaphragm type sampling device |
Non-Patent Citations (3)
| Title |
|---|
| Direct analysis of dried blood spots by in-line desorption combined with high-resolution chromatography and mass spectrometry for quantification of maple syrup urine disease biomarkers leucine and isoleucine;John H. Miller IV 等;《Anal Bioanal Chem》;20110208;第400卷;第237–244页 * |
| Direct analysis of dried blood spots coupled with mass spectrometry: concepts and biomedical applications;Julien Déglon 等;《Anal Bioanal Chem》;20110605;第402卷;第2485–2498页 * |
| 筛板对台锥形制备液相色谱柱流型和柱效的影响;马继平 等;《色谱》;20030731;第21卷(第4期);第430-433页 * |
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| CN104374850A (en) | 2015-02-25 |
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Effective date of registration: 20170104 Address after: 410205 Hunan province Changsha high tech Development Zone, Lu Tin Road No. 8 Oak Valley base Park 8 504-B, room 505-A Patentee after: HUNAN DEMETER INSTRUMENT CO.,LTD. Address before: 410011 Changsha City, Hunan province people's road, No. 139 Patentee before: Wang Feng |
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Denomination of invention: A diaphragm type sampling device Effective date of registration: 20230720 Granted publication date: 20160106 Pledgee: China Merchants Bank Co.,Ltd. Changsha Branch Pledgor: HUNAN DEMETER INSTRUMENT CO.,LTD. Registration number: Y2023980049129 |
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Granted publication date: 20160106 Pledgee: China Merchants Bank Co.,Ltd. Changsha Branch Pledgor: HUNAN DEMETER INSTRUMENT CO.,LTD. Registration number: Y2023980049129 |