CN104352850A - Extraction method of bamboo shavings extract - Google Patents
Extraction method of bamboo shavings extract Download PDFInfo
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- CN104352850A CN104352850A CN201410585553.8A CN201410585553A CN104352850A CN 104352850 A CN104352850 A CN 104352850A CN 201410585553 A CN201410585553 A CN 201410585553A CN 104352850 A CN104352850 A CN 104352850A
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- caulis bambusae
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- taenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- General Health & Medical Sciences (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to the technical field of medicines, in particular to an extraction method of bamboo shavings extract. The bamboo shavings extract obtained by the extraction method can be used for preparing antianxiety drugs. The bamboo shavings extract provided by the invention is capable of effectively treating anxiety.
Description
Technical field
The invention belongs to medical art, be specifically related to a kind of extracting method of Caulis Bambusae In Taenia extract.
Background technology
Anxiety neurosis (Anxiety) is a kind of neurosis based on anxiety, main manifestations is ictal or persistence anxiety, anxiety, terrified uneasiness etc., with a series of somatization and pathological change, pathogenesis is complicated, relates generally to the meticulous adjustment of neurotransmitter-receptor, neuromodulator-receptor, Neuroendocrine net.
Caulis Bambusae In Taenia is grass bambusa breviflora Munro
bambusa tuldoidesmunro, major part allusion quotation bamboo
sinocalamus beecheyanus(Munro) McClure var.
pubescensp.F.Li or phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle
phyllostachys nigra(Lodd.) Munro var.
henonis(Mitf.) the dry intermediate layer of the stem stalk of Stapf ex Rendle.Caulis Bambusae In Taenia clinical application is with a long history, begin to be loaded in " Medical Treasures of the Golden Chamber ", " bamboo skin " by name, its sweet in the mouth, cold nature, attach to the lung and stomach meridians, there is removing heat-phlegm, the function relieved restlessness and prevented vomiting, mainly containing the pentacyclic triterpenoid etc. that suberone, lupenone and homologue thereof are main, there is physiology and the pharmacologically actives such as free radical resisting, antioxidation, resisting fatigue, antitumor, blood pressure lowering, be mainly used in treatment dysphoria with smothery sensation vomiting, singultus, expectorant heat syndrome cough are breathed heavily, malignant histocytosis, fetal movement, infantile convulsion etc.But there is not been reported to prepare anxiolytic drugs with Caulis Bambusae In Taenia or Caulis Bambusae In Taenia extract.
Summary of the invention
The object of this invention is to provide a kind of extracting method of Caulis Bambusae In Taenia extract.The Caulis Bambusae In Taenia extract that this extracting method obtains may be used for preparing anxiolytic drugs.
In order to realize foregoing invention object, the invention provides following technical scheme:
The invention provides a kind of extracting method of Caulis Bambusae In Taenia extract, comprise the steps:
Step 1: get Caulis Bambusae In Taenia extracting in water, filtering and collecting filter liquid, being concentrated into relative density through first is 1.12 ~ 1.15, obtains the first extractum;
Step 2: get the mixed solution of described first extractum and chloroform and n-butyl alcohol through Sevage method deproteinization, collect water layer, being concentrated into relative density through second is 1.20 ~ 1.23, obtains the second extractum;
Step 3: get described second extractum and add ethanol precipitate with ethanol 20 ~ 24h that concentration is 80 ~ 95%, collected after centrifugation sediment, adds water stirring and dissolving, and being concentrated into relative density through the 3rd is 1.12 ~ 1.18, obtain the 3rd extractum, filtering and collecting filter liquid, to obtain final product; In g/mL, the mass volume ratio of described second extractum and ethanol is 1:1 ~ 4, and the mass volume ratio of described sediment and water is 1: 3 ~ 7.
Sevage method: the feature utilizing protein degeneration in the organic solvents such as trichloroethane, be to mix extracting solution and Sevage reagent [chloroform: n-butyl alcohol=5: 1 (V/V)] at 1: 1 according to volume ratio, vibration, centrifugal, the protein after degeneration is between extracting solution and Sevage reagent intersection.The advantage of this method is mild condition, can not cause the degeneration of polysaccharide.
In the present invention, described Caulis Bambusae In Taenia is grass bambusa breviflora Munro
bambusa tuldoidesmunro, major part allusion quotation bamboo
sinocalamus beecheyanus(Munro) McClure var.
pubescensp.F.Li or phyllostachys nigra (lodd.ex lindl.) munro var.henonis (miff.) spapf et rendle
phyllostachys nigra(Lodd.) Munro var.
henonis(Mitf.) the dry intermediate layer of the stem stalk of Stapf ex Rendle.
In step 1 of the present invention, in g/mL, the mass volume ratio of described Caulis Bambusae In Taenia and water is 1: 10 ~ 20.
Described first concentrated, the second concentrated or the 3rd simmer down to is at 70 ~ 80 DEG C, and vacuum is concentrated under the condition of 0.04 ~ 0.06MPa.
In described mixed solution, the volume ratio of chloroform and n-butyl alcohol is 5: 1.
The volume ratio of the first extractum and described mixed solution described in step 2 of the present invention is 1: 1.
After collecting water layer described in step 2, described second concentrated before also comprise step through 300 order filter-cloth filterings.
Centrifugal described in step 3 of the present invention is centrifugal 30min under the condition of 3000rpm.
Present invention also offers the Caulis Bambusae In Taenia extract that said extracted method obtains.
The Caulis Bambusae In Taenia extract that present invention also offers the acquisition of said extracted method is preparing the application in anxiolytic drugs.
The invention provides a kind of extracting method of Caulis Bambusae In Taenia extract.The Caulis Bambusae In Taenia extract that this extracting method obtains may be used for preparing anxiolytic drugs.Caulis Bambusae In Taenia extract provided by the invention effectively can treat anxiety neurosis.
Detailed description of the invention
In order to make those skilled in the art understand technical scheme of the present invention better, below in conjunction with specific embodiment, the present invention is described in further detail.
In the extracting method of Caulis Bambusae In Taenia extract provided by the invention, raw materials used and reagent all can be buied by market.
Embodiment 1:
Get Caulis Bambusae In Taenia medical material 100g, 10 times of water gagings are added after pulverizing, decoct 60 minutes, extracting liquid filtering, it is 80 DEG C in temperature, vacuum is be concentrated into relative density 1.15 (80 DEG C) under the condition of 0.06MPa, add the chloroform of same volume and the mixed solution (volume ratio of chloroform and n-butyl alcohol is 5: 1) of n-butyl alcohol, the flocculent deposit of removing middle white, water intaking layer is 80 DEG C in temperature, vacuum is that to be concentrated into relative density under the condition of 0.06MPa be 1.23 (80 DEG C), obtain extractum 18g, add 4 times amount 80% ethanol precipitate with ethanol, leave standstill 24 hours, collection sediment is centrifugal, reclaim ethanol, add 7 times of water gaging stirring and dissolving again, it is 80 DEG C in temperature, vacuum is be concentrated into relative density 1.18 (80 DEG C) under the condition of 0.06MPa, filter, spraying dry, obtain Caulis Bambusae In Taenia extract 4.5g.
Detect the Caulis Bambusae In Taenia extract obtained, result is as follows:
The discriminating of Caulis Bambusae In Taenia polysaccharide: discrimination method is phenolsulfuric acid reagent reacting (positive), containing Caulis Bambusae In Taenia polysaccharide in Caulis Bambusae In Taenia extract provided by the invention.
Caulis Bambusae In Taenia polyoses content: 47.1% (measuring with benzene phenol-sulfuric acid method) (with reference to " Chinese Pharmacopoeia " version in 2010 two)
Embodiment 2:
Get Caulis Bambusae In Taenia medical material 100g, 20 times of water gagings are added after pulverizing, decoct 40 minutes, extracting liquid filtering, it is 70 DEG C in temperature, vacuum is be concentrated into relative density 1.12 (80 DEG C) under the condition of 0.04MPa, add the chloroform of same volume and the mixed solution (volume ratio of chloroform and n-butyl alcohol is 5: 1) of n-butyl alcohol, the flocculent deposit of removing middle white, water intaking layer is 80 DEG C in temperature, vacuum is that to be concentrated into relative density under the condition of 0.05MPa be 1.20 (80 DEG C), obtain extractum 19g, add 3 times amount 95% ethanol precipitate with ethanol, leave standstill 24 hours, collection sediment is centrifugal, reclaim ethanol, add 5 times of water gaging stirring and dissolving again, it is 80 DEG C in temperature, vacuum is be concentrated into relative density 1.12 (80 DEG C) under the condition of 0.04MPa, filter, spraying dry, obtain Caulis Bambusae In Taenia extract 5.1g.
Detect the Caulis Bambusae In Taenia extract obtained, result is as follows:
The discriminating of Caulis Bambusae In Taenia polysaccharide: discrimination method is phenol-sulphate reagent reaction (positive), containing Caulis Bambusae In Taenia polysaccharide in Caulis Bambusae In Taenia extract provided by the invention.
Caulis Bambusae In Taenia polyoses content: 49.3% (measuring with benzene phenol-sulfuric acid method) (with reference to " Chinese Pharmacopoeia " version in 2010 two)
Embodiment 3:
Get Caulis Bambusae In Taenia medical material 100g, 15 times of water gagings are added after pulverizing, decoct 30 minutes, extracting liquid filtering, it is 75 DEG C in temperature, vacuum is be concentrated into relative density 1.13 (80 DEG C) under the condition of 0.05MPa, add the chloroform of same volume and the mixed solution (volume ratio of chloroform and n-butyl alcohol is 5: 1) of n-butyl alcohol, the flocculent deposit of removing middle white, collect water layer, through 300 order filter-cloth filterings, filtrate is 80 DEG C in temperature, vacuum is that to be concentrated into relative density under the condition of 0.04MPa be 1.22 (80 DEG C), obtain extractum 17g, add 1 times amount 80% ethanol precipitate with ethanol, leave standstill 24 hours, collection sediment is centrifugal, reclaim ethanol, add 3 times of water gaging stirring and dissolving again, it is 80 DEG C in temperature, vacuum is be concentrated into relative density 1.15 (80 DEG C) under the condition of 0.06MPa, filter, spraying dry, obtain Caulis Bambusae In Taenia extract 4.8g.
Detect the Caulis Bambusae In Taenia extract obtained, result is as follows:
The discriminating of Caulis Bambusae In Taenia polysaccharide: discrimination method is phenol-sulphate reagent reaction (positive), containing Caulis Bambusae In Taenia polysaccharide in Caulis Bambusae In Taenia extract provided by the invention.
Caulis Bambusae In Taenia polyoses content: 50.9% (measuring with benzene phenol-sulfuric acid method) (with reference to " Chinese Pharmacopoeia " version in 2010 two)
clinical trial:
One, physical data
60 routine anxiety patients, meet the diagnostic criteria of " Chinese Spirit Obstacles classification and diagnostic criteria " the 3rd edition anxiety neurosis, HAMA >=14 point.Without systemic disease, without drug dependence history and allergies, without brain organic disease.Get rid of gestation and women breast-feeding their children, get rid of and be associated with serious organ disease, get rid of the patients with depression with serious physical disease.Wherein male 27 example, women 33 example, 18 ~ 60 years old age, the course of disease 2 ~ 11 months, average course of disease 5.4 ± 1.5 months.
Two, Therapeutic Method
The Caulis Bambusae In Taenia extract that this group case all adopts oral embodiment 1 to prepare, every day three times, each 3 ~ 5g, takes 8 weeks continuously.Allow patient to use the improving water flood such as zolpidem, zopiclone, midazolam, alprazolam, clonazepam of routine dose simultaneously, take before all sleeping, use continuously and be no more than 2 weeks.But forbid other psychotropic drugs, systematic psychological therapy and electric convulsive treatment etc.
Three, therapeutic evaluation
Anxiety therapeutic evaluation adopts the Manifest Anxiety Scale (HAMA) of " Chinese Spirit Obstacles classification and diagnostic criteria " the 3rd edition, monitors inspection items such as routine blood test, hepatic and renal function, electrocardiograms simultaneously.Within 4th week and the 8th week, carry out therapeutic evaluation respectively before the treatment with after treatment.
Four, clinical effectiveness
This group case before the treatment after, HAMA total score score value significant difference (P< 0.05), after treatment, HAMA total score score value is on a declining curve, 4th week significant difference (P< 0.05), the 8th week difference highly significant (P < 0.01).Wherein the 4th week effective 34 examples, effective percentage 56.7%; 8th week accumulative effective 55 examples, accumulated effective rate 91.7%.
Caulis Bambusae In Taenia extract in prompting the present invention has significant therapeutic effect to anxiety neurosis.
Claims (9)
1. an extracting method for Caulis Bambusae In Taenia extract, is characterized in that, comprises the steps:
Step 1: get Caulis Bambusae In Taenia extracting in water, filtering and collecting filter liquid, being concentrated into relative density through first is 1.12 ~ 1.15, obtains the first extractum;
Step 2: get the mixed solution of described first extractum and chloroform and n-butyl alcohol through Sevage method deproteinization, collect water layer, being concentrated into relative density through second is 1.20 ~ 1.23, obtains the second extractum;
Step 3: get described second extractum and add ethanol precipitate with ethanol 20 ~ 24h that concentration is 80 ~ 95%, collected after centrifugation sediment, adds water stirring and dissolving, and being concentrated into relative density through the 3rd is 1.12 ~ 1.18, obtain the 3rd extractum, filtering and collecting filter liquid, to obtain final product; In g/mL, the mass volume ratio of described second extractum and ethanol is 1:1 ~ 4, and the mass volume ratio of described sediment and water is 1: 3 ~ 7.
2. according to the extracting method described in claim 1, it is characterized in that: in step 1, in g/mL, the mass volume ratio of described Caulis Bambusae In Taenia and water is 1: 10 ~ 20.
3. according to the extracting method described in claim 1, it is characterized in that: described first concentrated, the second concentrated or the 3rd simmer down to is at 70 ~ 80 DEG C, and vacuum is concentrated under the condition of 0.04 ~ 0.06MPa.
4. according to the extracting method described in claim 1, it is characterized in that: in described mixed solution, the volume ratio of chloroform and n-butyl alcohol is 5: 1.
5. according to the extracting method described in claim 1, it is characterized in that: the volume ratio of the first extractum and described mixed solution described in step 2 is 1: 1.
6., according to the extracting method described in claim 1, it is characterized in that, after collecting water layer described in step 2, described second concentrated before also comprise step through 300 order filter-cloth filterings.
7. according to the extracting method described in claim 1, it is characterized in that: centrifugal described in step 3 is centrifugal 30min under the condition of 3000rpm.
8. the Caulis Bambusae In Taenia extract that the extracting method provided according to any one of claim 1 to 7 obtains.
9. Caulis Bambusae In Taenia extract according to claim 1 is preparing the application in anxiolytic drugs.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410585553.8A CN104352850B (en) | 2014-10-28 | 2014-10-28 | The extracting method of Caulis Bambusae In Taenia extract |
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| CN201410585553.8A CN104352850B (en) | 2014-10-28 | 2014-10-28 | The extracting method of Caulis Bambusae In Taenia extract |
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| CN104352850A true CN104352850A (en) | 2015-02-18 |
| CN104352850B CN104352850B (en) | 2018-04-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998767A (en) * | 2016-05-20 | 2016-10-12 | 叶茂光 | Method for preparing bamboo shaving extract and bamboo pulp from bamboos |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228968A (en) * | 1998-03-17 | 1999-09-22 | 浙江农业大学 | Production method for extracting flavonoid compound extract or powder from bamboo leaf |
| CN101828750A (en) * | 2010-05-17 | 2010-09-15 | 浙江圣氏生物科技有限公司 | Method for extracting natural preservative from bamboo leaves |
-
2014
- 2014-10-28 CN CN201410585553.8A patent/CN104352850B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228968A (en) * | 1998-03-17 | 1999-09-22 | 浙江农业大学 | Production method for extracting flavonoid compound extract or powder from bamboo leaf |
| CN101828750A (en) * | 2010-05-17 | 2010-09-15 | 浙江圣氏生物科技有限公司 | Method for extracting natural preservative from bamboo leaves |
Non-Patent Citations (2)
| Title |
|---|
| 任美玲等: "竹叶多糖的研究现状与进展", 《食品工业科技》 * |
| 唐莉莉等: "竹叶多糖的分离提取及其生物活性研究", 《食品研究与开发》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998767A (en) * | 2016-05-20 | 2016-10-12 | 叶茂光 | Method for preparing bamboo shaving extract and bamboo pulp from bamboos |
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Inventor after: Yin Sheng Gao Inventor after: Xia Tian Inventor after: Xin Ning Inventor after: Guo Min Inventor after: Xin Hua Inventor after: Zhou Jiangyu Inventor after: Gong Mingshi Inventor after: Wang Liuping Inventor after: Li Hongju Inventor before: Yin Sheng Gao Inventor before: Xin Ning Inventor before: Guo Min Inventor before: Xin Hua Inventor before: Zhou Jiangyu Inventor before: Gong Mingshi Inventor before: Wang Liuping |
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Granted publication date: 20180427 Termination date: 20211028 |