CN104342367A

CN104342367A - Portable bacteriological detection system based on smart phone

Info

Publication number: CN104342367A
Application number: CN201310358917.4A
Authority: CN
Inventors: 刘星海
Original assignee: DRLIU XINGHAI; LIU XINGHAI DR
Current assignee: DRLIU XINGHAI; LIU XINGHAI DR
Priority date: 2013-08-07
Filing date: 2013-08-07
Publication date: 2015-02-11

Abstract

The invention discloses a portable bacteriological detection system based on a smart phone. A general bacteriological experiment includes lots of complex equipment and essential skills, which greatly hinder the application of microbiology. Based on a portable microbiological medium, the invention describes an instrument which can satisfy the overall process of growth, reservation and sterilization of bacteria, and extremely rigorous bio-safety measures are designed. According to the portable bacteriological detection system disclosed by the invention, the smart phone is used for simultaneously photographing and analyzing a multiple reference sample and a detection sample; if the analysis on a control group of which the results are known is wrong, the analysis of an analysis sample is not started, and thus wrong analysis is prevented, and the scientific preciseness of the analysis is greatly improved. Meanwhile, the system is a scientific researching and analyzing tool which can enable a commonly-educated person to finish scientific experiments under the training of smart phone software and to learn new skills.

Description

Based on the portable bacteriological detection system of smart mobile phone

Technical field

The test set more complicated that a basic bacteriology laboratory comprises.The present invention describes a household portable microorganism detection system that can be widely spread, and has science popularization and family expenses microbial standard detection double meaning.

Background technology

In order to the microbiological indicator in life can be detected, not only need the study of complicated science background knowledge and skills, also need very complicated Microbiological Lab's equipment and consumptive material (high-pressure steam sterilizing pan, aseptic operating platform, constant incubator, nutrient agar etc.).This hinders the universal of microorganism detection technology to a great extent.Since the eighties in last century, some portable microbiological culture medias have been had to appear on the market successively, the Petrifilm of such as Minnesota Mining and Manufacturing Company ^tMsystem (U.S. Patent number: 4,565,783), the scraps of paper completes microorganism culturing.However, with Petrifilm ^tMsupporting incubator and the equipment such as automatic intopretoscope (U.S. Patent number: US2010/0330610A1) and high pressure steam sterilization are not only expensive but also take up room large.Application still there is very large limitation.

Electrical heating element can rise to more than 170 degrees Celsius aluminum ingot temperature and enough kill microorganism, also the suitableeest culture temperature (such as 37 degrees Celsius) can be stabilized in, semiconductor refrigerating element can drop to 4 degrees Celsius aluminum ingot temperature, suppresses physiologically active, preserves fresh and alive detection sample.

Finally, present information science and technology is advanced by leaps and bounds, originally the computing power of a desktop computer, and present one can only just can complete by mobile phone.Add that modern smart mobile phone all inlays digital camera functionality.The present invention utilizes the digital imagery function of smart mobile phone and arithmetic capability to carry out treatment and analysis to experimental result.Although it should be noted that the digital camera that mobile phone is inlayed enters daily life, and go shooting science picture to need to coordinate a lot of parameter request with mobile phone, such as time shutter, aperture, focal length etc.Be difficult to a digital imaging technology being subject to the personnel of few training to grasp multinomial requirement very soon, be therefore badly in need of developing a kind of method and allow user interface and the more simple product of use procedure.

Summary of the invention

The present invention includes a temperature control aluminium ingot device, be used for the growth of bacterium, preserve and the temperature control all processes of sterilizing.Also comprise and utilize the electronic imaging in smart mobile phone and computing function to carry out fundamental analysis to bacterial growth media.Specifically comprise following five aspects:

The first, in the cabin body that take aluminium ingot as heat-conducting medium, utilize the characteristic of mica electrically heated, semiconductor refrigerating and electronic temperature control, strict temperature and the control of interval time are carried out to aluminium ingot heat conduction cabin body, comes with this:

A. reach optimum growth temperature (such as 37 degrees Celsius), encourage microbial growth;

B. (such as grow 24 hours) after reaching the growth time of necessity, system reduces temperature automatically to resting stage (such as 4 degrees Celsius), so that next step Micro biological Tests;

C. where necessary, temperature can rise to more than 170 degrees Celsius, carrys out all microorganisms of deactivation, reach the object of Biosafety with this.

The second, use the photographic camera in smart mobile phone to carry out imaging to portable microbiological culture media, and use the computing function of smart mobile phone to carry out computing, analysis and deciphering to image.

3rd, use the quality of multiple contradistinction system to shot detection sample photo to assess, these are the print of known experimental result to photograph and picture usually.

4th, use the internet of smart mobile phone, popularity science popularization explanation and interchange are carried out to detected result.

5th, this invention has science popularization adult toy function, allows the grownup being subject to elementary education, utilizes smart mobile phone at hand and simple equipment, has the hardware facility that some base microorganisms are studied, the Microbiological Lab's equipment replacing numerous and diverse.

Accompanying drawing explanation

Fig. 1: adopt multiple print matter control group to analyze micro-biological samples.Wherein contrast the print that 1,2,3,4 are all known experimental result respectively.

embodiment

This experiment mainly employs following material:

1. Bright brand pasteurized milk in Shanghai is buied by agriculture, industry and commerce supermarket, Shanghai City;

2.Petrifilm ^tMcoliform testing plate (ordering code 6416) portable microbiological culture media is buied by Minnesota Mining and Manufacturing Company;

3. smart mobile phone Huawei mate Ascend MT1-U06 is buied by Shenzhen Huawei Company;

4. Rong Cheng board mica electric heater is buied by Shanghai Rong Cheng Thermal Power Co., Ltd;

5. Te Luonai board arrangement for semiconductor electronic refrigeration chips TEC1-12702 is buied by Shenzhen Mi Shang company;

6. electronic thermometer (article No. TP3001, range is-50 to 300 degrees Celsius) is buied by Zhengzhou Bo Yang instrument company limited;

7. aluminium sheet is buied (thickness: 2.5 millimeters, length and width: 8 centimetres, operative norm: GB/T11962008) by South Mountain, Shandong Aluminum;

8. temperature control device REX-C100 intelligent temperature controller (0-400 degree Celsius) is bought by Heng Yuan instrucment and meter plant of From Yueqing County, Zhejiang city.

Operation steps:

1. on aluminium sheet, make a call to the hole that a diameter is 1 millimeter, keep the micro-aerobic environment of bacterium;

2. refrigeration, heating and electronic temperature control are fixed on aluminium sheet according to product description, debug 3 degrees Celsius, 25 degrees Celsius and 180 degrees Celsius of three temperature spots, use temperature meter calibrating, and confirm stability;

3. take out multiple known Petrifilm ^tMportable microbiological culture media experimental result picture, these picture photocopy and to be printed onto on same a piece of paper and reserving space to detecting sample, pre-aligned good control group and the physical location of detection sample on photo, and record these positional informations, the position of each control group of training smart handset identity and the position of detection sample, if have selected 4 groups of contrasts (lower same), as shown in Figure 1.

4. according to the Petrifilm of 3M company ^tMspecification sheets detects the coliform of pasteurized milk;

5. portable for survey microbiological culture media is put into aluminium ingot, temperature control 37 degrees Celsius 24 hours;

6. the portion that connects after 24 hours aluminium ingot temperature control be at once adjusted to 4 degrees Celsius to be detected;

7. connect in 48 hours of step, the portable microbiological culture media of sample, puts as shown in Figure 1, comprises control group, takes a picture, comprise whole five substratum in same photo with smart mobile phone;

8. the morphological feature in the arithmetic system scanned picture of smart mobile phone is (as used Petrifilm ^tMit is the bacterium colony color darker than substratum that coliform testing plate orders the optical signature of code 6416, is counted as 1 point);

9. smart mobile phone is through picture scanning, record, storage, analysis, contrast, if multiple control group picture analyzing result is with known set result is inconsistent in advance, judges to detect samples pictures shooting defective, again operates from the 8th step.

10. smart mobile phone is through picture scanning, record, storage, analysis, contrast, if multiple control group picture analyzing result is with to preset result consistent, judges to detect samples pictures shooting qualified, continues the 11st step.

11. computers, to the scanning of detection samples pictures, record, storage, analysis, contrast, draw the microbiological parameters and index that detect sample, according to parameter generation examining report.

Portable microbiological culture media complete is by analysis put into a plastics photo sealer bag by 12., then puts it into aluminium ingot temperature control 170 degree of 2 hours dry sterilizations.

Sample after dry sterilization is discarded into rubbish container by 13..

Claims

1. a Temperature-controlled appliance, utilizes the good conductor (as: copper, aluminium or alloy etc.) of heat to coordinate temperature-control device to complete whole temperature requirements of general bacteriology experiment.

2. claim 1 equipment can: be adjusted to specified temp and promote bacterial growth (usual 20-40 degree Celsius), with the physiologically active (usual 0-15 degree Celsius) of a specified temp anti-bacteria, and kill bacteria (usual more than 100 degrees Celsius).

3. the equipment of claim 1 can also produce high temperature, enough makes thermoset plastic bag solidify, and is locked in plastics bag by the portable substratum after sterilizing, not easily removes or Kaifeng, guarantees the possibility of the substratum after using without any biological pollution.

4. the equipment of claim 1 according to specific experiment requirement, can also carry out each physiological stage described by (or part completes) claim 2 with given time and program.

5. one or multiple control group microbiological culture media pictures must be comprised in the photo of shot detection biological medium.

6. use the photo of intelligent mobile phone system to claim 5 to analyze, sample and controlled analysis must be comprised.

7. the detected object analysis of pair claim 5 must be based upon on the exact basis of controlled analysis result.

8. smart mobile phone can set up instruction relation with equipment described in claim 1 by radio magnetic wave or wired mode.