CN104328047A - Clamp structure for deep-pore tissue culture plate - Google Patents
Clamp structure for deep-pore tissue culture plate Download PDFInfo
- Publication number
- CN104328047A CN104328047A CN201410590095.7A CN201410590095A CN104328047A CN 104328047 A CN104328047 A CN 104328047A CN 201410590095 A CN201410590095 A CN 201410590095A CN 104328047 A CN104328047 A CN 104328047A
- Authority
- CN
- China
- Prior art keywords
- deep
- culture plate
- rotating shaft
- cell culture
- base plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011148 porous material Substances 0.000 title abstract 5
- 238000004113 cell culture Methods 0.000 claims description 26
- 238000012856 packing Methods 0.000 claims description 5
- 238000011109 contamination Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a clamp structure for a deep-pore tissue culture plate. The clamp structure is capable of compressing and fixing the deep-pore tissue culture plate on a shaker to ensure the air tightness of connection between a plate cover of the deep-pore tissue culture plate and the main body of the deep-pore tissue culture plate. The clamp structure comprises rotating shafts, shifting sheets, eccentric gears and brackets, wherein the two ends of each rotating shaft are supported by virtue of a group of brackets, each rotating shaft is fixedly connected with two eccentric shafts, the shifting sheets are fixedly connected to the rotating shafts, and each shifting sheet and the corresponding rotating shaft are arranged into a T shape.
Description
Technical field
The present invention relates to cell culture apparatus technical field, particularly relate to the deep hole culture plate of cell cultures, be specially the structure of Deep-hole cell culture plate fixture.
Background technology
Along with molecular biological development, the heterogenous expression technology of albumen is ripe gradually and be widely used.The expression of foreign protein needs to select suitable expressive host and Expression element, and this just needs to build huge expression library, therefrom selects preferably representation aids box.Deep-hole cell culture plate can utilize less nutrient solution to carry out the screening of high-flux cell cultivation and bacterial classification, cell strain and expression library, has fast, high-throughput, the feature such as cost-saving.
Existing deep hole Deep-hole cell culture plate is in use in order to avoid crossed contamination between microbiological contamination or plate hole, need to seal plate mouth, now mainly contain two kinds of sealing plate mouth modes, one is that latex mattress is sealed, one is the sealing of " sandwich " formula plate lid, and latex mattress cannot carry out gaseous interchange in use, and stopping property is poor, and " sandwich " formula plate lid well can carry out gaseous interchange, avoid crossed contamination.But stopping property cannot ensure when plate lid and deep-well plates are combined, and need specifically to cultivate shaking table.
Summary of the invention
For the problems referred to above, patent of the present invention provides the structure of Deep-hole cell culture plate fixture, and Deep-hole cell culture plate can be fixed on shaking table by effectively, guarantees the stopping property that deep-well cell culture plate cover is connected with culture plate main body.
Its technical scheme is such, and it comprises rotating shaft, plectrum, eccentric wheel and support, and described rotating shaft two ends are supported by support described in a group, and described rotating shaft is connected with eccentric wheel, and described plectrum is fixed in described rotating shaft and setting in T shape with described rotating shaft.
Further, described eccentric radially outer edge face is provided with a circle groove, is provided with packing ring in described groove;
Further, be positioned at inside support, described rotating shaft two ends are respectively connected with an eccentric wheel;
Further, also comprise base plate, described support is fixed on described base plate, and described base plate is provided with mounting block, and described mounting block is for being fixed on shaking table by base plate;
Further, be positioned at and often organize immediately below described support, described base plate is provided with a groove, and described groove mates with Deep-hole cell culture plate;
Further, described base plate both sides are connected with handle;
Further, described plectrum is substituted with driving lever.
After adopting structure of the present invention, its beneficial effect is: before shaking table is cultivated, first Deep-hole cell culture plate is positioned in groove, then plectrum is rotated, eccentric wheel rotates, utilize eccentric eccentric action Deep-hole cell culture plate to be fixed, guarantee the stopping property that deep-well cell culture plate cover is connected with culture plate main body, avoid cell in culturing process to cause microbiological contamination and crossed contamination because nutrient solution splashes; Eccentric wheel radially outer edge face is provided with packing ring, and this packing ring can not only strengthen the frictional force between eccentric wheel and cover plate, prevents eccentric wheel from loosening, and can effectively avoid plate cap surface to wear and tear; Base plate arranges groove, realizes the spacing placement of Tissue Culture Plate, and the pressure face of touching of eccentric wheel and cover plate is between adjacent two row cell cultures deep holes, guarantee that deep-well cell culture plate cover has good gaseous interchange effect; Base plate is provided with anchor, and can mate with common shaking table, suitability is wide.
Accompanying drawing explanation
Fig. 1 is the structural representation of one embodiment of the invention;
Fig. 2 is the left TV structure schematic diagram of Fig. 1;
Fig. 3 is eccentric structural representation of the present invention.
Embodiment
As shown in Figure 1, Figure 2 and Figure 3, comprise rotating shaft 6, plectrum 9, eccentric wheel 7 and support 5, rotating shaft 6 two ends are supported by a pack support 5, be positioned at inside support 5, rotating shaft 6 two ends are respectively connected with an eccentric wheel 7, eccentric wheel 7 bias is connected in rotating shaft 6, and plectrum 9 is fixed in rotating shaft 6 and setting in T shape with rotating shaft 6; The radially outer edge face of eccentric wheel 7 is provided with a circle groove 10, is provided with O type packing ring 8 in groove 10; Also comprise base plate 3, support 5 is fixed on base plate 3, and base plate 3 is provided with mounting block, mounting block is bolt, is positioned at immediately below every pack support, and base plate 3 is provided with a groove 11, groove 11 mates with Deep-hole cell culture plate 4, and in figure, 2 is the bolt hole of erection bolt, for base plate 3 is fixed on shaking table; Base plate 3 both sides are connected with handle (not shown).
Specific operation process is as follows:
First by adding substratum, inoculated culture plate is positioned in the groove 11 of base plate, place appropriate after, rotate plectrum 9, drive the eccentric wheel 7 in rotating shaft 6 to rotate, until eccentric wheel 7 pressure strip lid; Then base plate 3 is put into shaking table, and be secured by bolts in shaking table plate face 1, as shown in Figure 1; Run shaking table, carry out cell cultures.
Claims (7)
1. the structure of Deep-hole cell culture plate fixture, comprise rotating shaft, plectrum, eccentric wheel and support, described rotating shaft two ends are supported by support described in a group, and described rotating shaft is connected with eccentric wheel, and described plectrum is fixed in described rotating shaft and setting in T shape with described rotating shaft.
2. the structure of Deep-hole cell culture plate fixture according to claim 1, is characterized in that: be positioned at inside support, described rotating shaft two ends are respectively connected with an eccentric wheel.
3. the structure of Deep-hole cell culture plate fixture according to claim 1 and 2, is characterized in that: described eccentric radially outer edge face is provided with a circle groove, is provided with packing ring in described groove.
4. the structure of Deep-hole cell culture plate fixture according to claim 3, is characterized in that: also comprise base plate, and described support is fixed on described base plate, and described base plate is provided with mounting block, and described mounting block is for being fixed on shaking table by base plate.
5. the structure of Deep-hole cell culture plate fixture according to claim 4, is characterized in that: be positioned at and often organize immediately below described support, described base plate is provided with a groove, and described groove mates with Deep-hole cell culture plate.
6. the structure of Deep-hole cell culture plate fixture according to claim 5, is characterized in that: described base plate both sides are connected with handle.
7. the structure of the Deep-hole cell culture plate fixture according to claim 1,2,4,5 or 6, is characterized in that: substitute described plectrum with driving lever.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410590095.7A CN104328047B (en) | 2014-10-29 | Deep-hole cell culture plate fixture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410590095.7A CN104328047B (en) | 2014-10-29 | Deep-hole cell culture plate fixture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104328047A true CN104328047A (en) | 2015-02-04 |
| CN104328047B CN104328047B (en) | 2017-01-04 |
Family
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619787A (en) * | 2017-11-10 | 2018-01-23 | 安徽金思源生物科技有限公司 | Constant temperature shaking table for biological laboratory |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003093044A (en) * | 2001-09-21 | 2003-04-02 | Sumitomo Bakelite Co Ltd | Method for packaging culture vessel and method for using the same |
| CN201459104U (en) * | 2009-03-05 | 2010-05-12 | 华东理工大学 | Micro-porous plate fixing device |
| CN102406496A (en) * | 2011-11-08 | 2012-04-11 | 龙刚 | Steering and locating mechanism for endoscope |
| US20120164725A1 (en) * | 2009-09-05 | 2012-06-28 | Matt Stettler | Deepwell plate system with lid |
| CN102941554A (en) * | 2012-12-10 | 2013-02-27 | 重庆天之道科技发展有限公司 | Clamping device of eccentric cam |
| CN204325373U (en) * | 2014-10-29 | 2015-05-13 | 江南大学 | A kind of structure of Deep-hole cell culture plate fixture |
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003093044A (en) * | 2001-09-21 | 2003-04-02 | Sumitomo Bakelite Co Ltd | Method for packaging culture vessel and method for using the same |
| CN201459104U (en) * | 2009-03-05 | 2010-05-12 | 华东理工大学 | Micro-porous plate fixing device |
| US20120164725A1 (en) * | 2009-09-05 | 2012-06-28 | Matt Stettler | Deepwell plate system with lid |
| CN102406496A (en) * | 2011-11-08 | 2012-04-11 | 龙刚 | Steering and locating mechanism for endoscope |
| CN102941554A (en) * | 2012-12-10 | 2013-02-27 | 重庆天之道科技发展有限公司 | Clamping device of eccentric cam |
| CN204325373U (en) * | 2014-10-29 | 2015-05-13 | 江南大学 | A kind of structure of Deep-hole cell culture plate fixture |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619787A (en) * | 2017-11-10 | 2018-01-23 | 安徽金思源生物科技有限公司 | Constant temperature shaking table for biological laboratory |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170104 |