CN104304173A - Rapid detection method and system for chicken embryo stress reaction on illumination - Google Patents
Rapid detection method and system for chicken embryo stress reaction on illumination Download PDFInfo
- Publication number
- CN104304173A CN104304173A CN201410622878.9A CN201410622878A CN104304173A CN 104304173 A CN104304173 A CN 104304173A CN 201410622878 A CN201410622878 A CN 201410622878A CN 104304173 A CN104304173 A CN 104304173A
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- chicken embryo
- illumination
- light source
- cylindricality
- stress reaction
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- 241000287828 Gallus gallus Species 0.000 title claims abstract description 110
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 101
- 238000005286 illumination Methods 0.000 title claims abstract description 36
- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims description 14
- 240000005528 Arctium lappa Species 0.000 claims description 7
- 229940012466 egg shell membrane Drugs 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 6
- 238000011160 research Methods 0.000 abstract description 9
- 238000013461 design Methods 0.000 abstract description 2
- 230000012447 hatching Effects 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract 4
- 210000002257 embryonic structure Anatomy 0.000 abstract 3
- 102000002322 Egg Proteins Human genes 0.000 abstract 1
- 108010000912 Egg Proteins Proteins 0.000 abstract 1
- 210000003278 egg shell Anatomy 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000012496 stress study Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
- A01K45/007—Injecting or otherwise treating hatching eggs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a rapid detection method and a system for chicken embryo stress reaction on illumination. The rapid detection method for the chicken embryo stress reaction on illumination comprises constructing a cylinder experiment area, keeping the environment of the cylinder experiment area and the environment of an incubator to be consistent, maintaining constant temperature and constant moisture, installing a light source in the middle inside the lightproof cylinder experiment area, putting chicken embryos into the cylinder experiment area, installing a voice sensor in a chicken embryo air chamber small hole, pasting the voice sensor on an eggshell film inside the small hole, converting signals received by the voice sensor and obtaining heartbeat signals of chicken embryos, changing an illumination condition of the chicken embryos by adjusting the light source intensity and the wave length and the chicken embryo height position and comparing after detection for a plurality of times and obtaining the chicken embryo stress reaction on the illumination. The rapid detection method for the chicken embryo stress reaction on illumination is mainly used for monitoring the chicken embryo stress reaction on the illumination, is simple in design and reasonable in structure, guarantees the temperature and the moisture to be consistent during a hatching process, changing the wave length and the light intensity of the LED (Light Emitting Diode) light source, obtaining the change of chicken embryo heartbeat signals under different illumination conditions and deepening a rasorial stress influence research.
Description
Technical field
The present invention relates to a kind of detection method and system of chicken embryo, especially relate to a kind of chicken embryo to the method for quick of illumination stress reaction and system.
Background technology
In current poultry cultivation, the intensive process inherently increasing stress reaction of bird industry.Due to the effect of stressors, make the physiological environment in bird body and adaptability, stability changes, and creates new adaptation, to sustain life and restore funcitons.Bird stress in, stress study particularly to chicken, has significant impact for the production of chicken, physiology, behavior.And just the stress reaction of chicken is studied when chicken embryo, the growth of later chicken is had to the effect of novelty more.LED light source relies on volume little, and the advantages such as the life-span is long, changeable have been widely used in the every field such as various instruction, display, decoration, backlight, general lighting and urban landscape.
In current existing research mode, mostly the research of scholars is the independent research to chicken embryo heartbeat detection, defining not to environment, or object is the research of ripe chicken physiological activity under different illumination conditions, lacks under the defining of environment the research of chicken embryo heartbeat detection and the research of chicken embryo physiological activity under different illumination conditions.
Summary of the invention
In order to studying a question of chicken embryo heartbeat detection can be solved, the object of the present invention is to provide a kind of chicken embryo to the method for quick of illumination stress reaction and system, detect environmental lighting conditions stress reaction to external world.
The technical solution adopted for the present invention to solve the technical problems is:
One, a kind of chicken embryo is to the method for quick of illumination stress reaction:
Build a cylindricality Experimental Area, the environment of its environment and incubator is consistent, keep constant temperature and constant humidity, cylindricality Experimental Area is light tight, in cylindricality Experimental Area, light source is equipped with at middle part, chicken embryo is put into cylindricality Experimental Area, the varying height positions joint of chicken embryo, an aperture is had in the air chamber position of chicken embryo, the sound transducer for detecting chicken embryo voice signal aperture is equipped with, sound transducer is pasted onto in the egg shell membrane in aperture, the signal received by sound transducer is converted to the heartbeat signal of chicken embryo, the intensity of light source and the height and position of wavelength and chicken embryo is regulated to change the illumination condition of chicken embryo, under the illumination condition of different chicken embryo, then repeated detection is compared and is obtained the stress reaction of chicken embryo to illumination.
The height of described chicken embryo is regulated by the chicken embryo fixed mount be installed in cylindricality Experimental Area.
The outside of described cylindricality Experimental Area adopts light-proof material to hide to isolate ambient light photograph.
Described light-proof material is fluorescent cloth or gobo.
Two, a kind of chicken embryo is to the rapid detection system of illumination stress reaction:
Comprise cylindricality Experimental Area, light source fixing frame, light source controller, light source sound transducer, A/D converter circuit, calculator, thermometer, thermostatically-controlled equipment and chicken embryo fixed mount; Cylindricality Experimental Area is placed in thermostatically-controlled equipment, and lateral surface is all coated with light-proof material to isolate ambient light photograph, and cylindricality is provided with thermometer in Experimental Area; Light source is arranged in the middle part of the madial wall of cylindricality Experimental Area by light source fixing frame, and is connected with the light source controller outside cylindricality Experimental Area, by light source controller adjusting wavelength and light intensity; Chicken embryo fixed mount is arranged on the center of cylindricality Experimental Area, chicken embryo is positioned on chicken embryo fixed mount, the air chamber position of chicken embryo has an aperture, the sound transducer for detecting chicken embryo aperture is equipped with, sound transducer is pasted onto in the egg shell membrane in aperture, and sound transducer is connected with calculator through A/D converter circuit.
Described sound transducer is electret microphone.
Described thermostatically-controlled equipment is the tank keeping constant temperature.
Described chicken embryo fixed mount is the fixed mount that can be elevated.
Described light-proof material is fluorescent cloth or gobo.
The beneficial effect that the present invention has is:
1, the present invention is mainly used in monitoring chicken embryo to the stress reaction of illumination, simplicity of design, rational in infrastructure.
2, present system is passed through, both ensure that temperature in hatching process, humidity were consistent, achieved the principle controlling unitary variant, by changing wavelength and the light intensity of LED light source, can obtain the change of chicken embryo heartbeat signal under different illumination conditions, be namely stress to the in-depth of the influence research of poultry.
3, the adjustable egg rack of the present invention regulates chicken embryo position, can record the heartbeat of different chicken embryo position thus, for the further research of later different chicken embryo position provides more accurate data.
Accompanying drawing explanation
Fig. 1 is the johning knot composition of present system.
Fig. 2 is the process schematic of measuring method of the present invention.
In figure: 1, cylindricality Experimental Area, 2, light source fixing frame, 3, light source controller, 4, light source, 5, light-proof material, 6, chicken embryo, 7, aperture, 8, sound transducer, 9, A/D converter circuit, 10, calculator, 11, thermometer, 12, water, 13, thermostatically-controlled equipment, 14, chicken embryo fixed mount.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.
As shown in Figure 2, the inventive method comprises: first build a cylindricality Experimental Area, the environment of its environment and incubator is consistent, keep constant temperature and constant humidity, cylindricality Experimental Area is light tight, in cylindricality Experimental Area, middle part is provided with light source, chicken embryo is put into cylindricality Experimental Area, the varying height positions joint of chicken embryo, an aperture is had in the air chamber position of chicken embryo, the sound transducer for detecting chicken embryo voice signal aperture is equipped with, sound transducer is pasted onto in the egg shell membrane in aperture, the signal received by sound transducer is connected the heartbeat signal being converted to chicken embryo with calculator by A/D converter circuit, light source can connect light source controller and carry out adjusting wavelength and luminous intensity (the i.e. color of adjustment light source 4, brightness), empirically require to regulate the intensity of light source and the height and position of wavelength and chicken embryo to change the illumination condition of chicken embryo, under the illumination condition of different chicken embryo, then repeated detection is compared and is obtained the stress reaction of chicken embryo to illumination.
The height of chicken embryo is regulated by the chicken embryo fixed mount be installed in cylindricality Experimental Area.
The outside of cylindricality Experimental Area adopts light-proof material to hide to isolate ambient light photograph, and light-proof material can adopt fluorescent cloth or gobo.
As shown in Figure 1, present system comprises cylindricality Experimental Area 1, light source fixing frame 2, light source controller 3, light source 4 sound transducer 8, A/D converter circuit 9, calculator 10, thermometer 11, thermostatically-controlled equipment 13 and chicken embryo fixed mount 14; Cylindricality Experimental Area 1 is placed in thermostatically-controlled equipment 13, and lateral surface is all coated with light-proof material 5 to isolate ambient light photograph, is provided with thermometer 11 in cylindricality Experimental Area 1; Light source 4 is fixedly mounted in the middle part of the madial wall of cylindricality Experimental Area 1 by light source fixing frame 2, and is connected with the light source controller 3 outside cylindricality Experimental Area 1, by light source controller 3 adjusting wavelength and light intensity; Chicken embryo fixed mount 14 is arranged on the center of cylindricality Experimental Area 1, chicken embryo 6 is positioned on chicken embryo fixed mount 14, the air chamber position of chicken embryo 6 has an aperture 7, the sound transducer 8 for detecting chicken embryo aperture is equipped with, sound transducer 8 is pasted onto in the egg shell membrane in aperture, and sound transducer 8 is connected with calculator 10 through A/D converter circuit 9.
Preferred sound transducer 8 is electret microphone.
Preferably, cylindricality Experimental Area can adopt beaker, the heat that cylindricality Experimental Area can be conducted from thermostatically-controlled equipment 13 maintains constant temperature, thermostatically-controlled equipment 13 adopts the water-bath keeping constant temperature, water-bath is built with water 12, the temperature in cylindricality Experimental Area 1 is made to keep constant temperature, by thermometer 11 displays temperature in cylindricality Experimental Area 1.
Chicken embryo fixed mount 14 is the fixed mount that can be elevated, and because light source position is fixed, by lifting chicken embryo, light source irradiation can be adjusted to the position of chicken embryo and angle, realize the testing requirement of various different illumination conditions.
Preferred light source is LED light source, and light-proof material can adopt fluorescent cloth or gobo.
Embodiments of the invention and implementation process as follows:
As shown in Figure 1, cylindricality Experimental Area 1 adopts the beaker of energy heat conduction, beaker is 1L, outside is all coated with light-proof material 5 for gobo is to isolate ambient light photograph, and be placed in water-bath, beaker inside is provided with mercurial thermometer 11, make the Temperature displaying of mercurial thermometer at about 37.5 DEG C by regulating water-bath, the LED that the wavelength brightness that light source 4 adopts Lang Tuo bio tech ltd to produce is adjustable, be fixed on beaker sidewall, LED is fixed on distance beaker bottom 10cm on the sidewall of beaker 1 by light source fixing frame 2, beaker height 15.5cm, light source controller 3 is regulated to make LED4 to wavelength 560 nm, light intensity 30 Lux, the embryo of the 17th day is knocked on air chamber direction open the aperture 7 of an about 1cm*1cm, make electret microphone 8 can just be arranged on aperture 7, electret microphone 8 is connected by double faced adhesive tape with egg shell membrane, regulate chicken embryo fixed mount height 14 to distance beaker bottom 5cm, light source 4 is made to impinge upon chicken embryo 6 top, observed and recorded calculator 10 signal, regulate chicken embryo fixed mount 14 height to distance beaker bottom 7.5cm after 5 minutes again, detection light source 4 impinges upon the output signal of computer-chronograph in the middle part of chicken embryo 6, same mistake 5 minutes, regulate chicken embryo fixed mount 14 height to distance beaker bottom 10cm, when detection light source 4 impinges upon bottom chicken embryo 6, signal also will collate the minutes with group three signals and obtain first group of data.Change the wavelength of light source 4 to 450nm, regulate light source controller to above three positions, obtain second group of data.Light intensity to 15 Lux is changed when wavelength is 560nm, regulate light source controller to above three positions, obtain the 3rd group of data, by comparing first group and second group of data show that the long lower chicken blastophore of Different lightwave is jumped signal and distinguished thus can obtain chicken embryo to the stress reaction of optical wavelength and rule thereof.The chicken blastophore drawing under different light intensity by comparing first group and the 3rd group of data jump signal difference thus can release chicken embryo to light intensity stress rule.
Above-mentioned embodiment is used for explaining and the present invention is described, instead of limits the invention, and in the protection domain of spirit of the present invention and claim, any amendment make the present invention and change, all fall into protection scope of the present invention.
Claims (9)
1. a chicken embryo is to the method for quick of illumination stress reaction, it is characterized in that comprising: build a cylindricality Experimental Area, the environment of its environment and incubator is consistent, keep constant temperature and constant humidity, cylindricality Experimental Area is light tight, in cylindricality Experimental Area, light source is equipped with at middle part, chicken embryo is put into cylindricality Experimental Area, the varying height positions joint of chicken embryo, an aperture is had in the air chamber position of chicken embryo, the sound transducer for detecting chicken embryo voice signal aperture is equipped with, sound transducer is pasted onto in the egg shell membrane in aperture, the signal received by sound transducer is converted to the heartbeat signal of chicken embryo, the intensity of light source and the height and position of wavelength and chicken embryo is regulated to change the illumination condition of chicken embryo, under the illumination condition of different chicken embryo, then repeated detection is compared and is obtained the stress reaction of chicken embryo to illumination.
2. a kind of chicken embryo according to claim 1 is to the method for quick of illumination stress reaction, it is characterized in that: the height of described chicken embryo is regulated by the chicken embryo fixed mount be installed in cylindricality Experimental Area.
3. a kind of chicken embryo according to claim 1 is to the method for quick of illumination stress reaction, it is characterized in that: the outside of described cylindricality Experimental Area adopts light-proof material to hide to isolate ambient light photograph.
4. a kind of chicken embryo according to claim 3 is to the method for quick of illumination stress reaction, it is characterized in that: described light-proof material is fluorescent cloth or gobo.
5., for implementing the claims a kind of chicken embryo of method described in 1 ~ 4 to the rapid detection system of illumination stress reaction, it is characterized in that: comprise cylindricality Experimental Area (1), light source fixing frame (2), light source controller (3), light source (4) sound transducer (8), A/D converter circuit (9), calculator (10), thermometer (11), thermostatically-controlled equipment (13) and chicken embryo fixed mount (14); Cylindricality Experimental Area (1) is placed in thermostatically-controlled equipment (13), and lateral surface is all coated with light-proof material (5) to isolate ambient light photograph, is provided with thermometer (11) in cylindricality Experimental Area (1); Light source (4) is arranged in the middle part of cylindricality Experimental Area (1) madial wall by light source fixing frame (2), and is connected with cylindricality Experimental Area (1) light source controller outward (3), by light source controller (3) adjusting wavelength and light intensity; Chicken embryo fixed mount (14) is arranged on the center of cylindricality Experimental Area (1), chicken embryo (6) is positioned on chicken embryo fixed mount (14), the air chamber position of chicken embryo (6) has an aperture (7), the sound transducer (8) for detecting chicken embryo aperture is equipped with, sound transducer (8) is pasted onto in the egg shell membrane in aperture, and sound transducer (8) is connected with calculator (10) through A/D converter circuit (9).
6. a kind of chicken embryo according to claim 5 is to the rapid detection system of illumination stress reaction, it is characterized in that: described sound transducer (8) is electret microphone.
7. a kind of chicken embryo according to claim 5 is to the rapid detection system of illumination stress reaction, it is characterized in that: described thermostatically-controlled equipment (13) is the tank keeping constant temperature.
8. a kind of chicken embryo according to claim 5 is to the rapid detection system of illumination stress reaction, it is characterized in that: described chicken embryo fixed mount (14) is the fixed mount that can be elevated.
9. a kind of chicken embryo according to claim 5 is to the rapid detection system of illumination stress reaction, it is characterized in that: described light-proof material (5) is fluorescent cloth or gobo.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410622878.9A CN104304173B (en) | 2014-11-08 | 2014-11-08 | Method for quick and the system of chicken embryo to illumination stress reaction |
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| CN201410622878.9A CN104304173B (en) | 2014-11-08 | 2014-11-08 | Method for quick and the system of chicken embryo to illumination stress reaction |
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| CN104304173A true CN104304173A (en) | 2015-01-28 |
| CN104304173B CN104304173B (en) | 2016-05-18 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105165660A (en) * | 2015-10-20 | 2015-12-23 | 山东大学齐鲁医院 | Incubated living body egg embryo fetal monitoring device |
| CN105191836A (en) * | 2015-08-21 | 2015-12-30 | 中国海洋大学 | Scallop heartbeat index based rapid seed selection method |
| RU2665117C2 (en) * | 2016-04-18 | 2018-08-28 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "РОССИЙСКИЙ ГОСУДАРСТВЕННЫЙ АГРАРНЫЙ ЗАОЧНЫЙ УНИВЕРСИТЕТ" | Method for recording the heart rate of a bird embryo without the shell destruction and the device for its implementation |
| CN108986066A (en) * | 2018-04-23 | 2018-12-11 | 中国农业科学院特产研究所 | A kind of detection method and system of chicken embryo |
| CN108990902A (en) * | 2018-09-03 | 2018-12-14 | 薄文海 | A kind of method for breeding improving pigeon eggs hatching rate |
| CN109819941A (en) * | 2019-04-04 | 2019-05-31 | 中国农业大学 | It is a kind of conducive to hatching and its later period chicken group healthy growth hatching illumination method |
| CN113575467A (en) * | 2021-07-29 | 2021-11-02 | 福州木鸡郎智能科技有限公司 | Block chain algorithm-based egg positioning method suitable for free-range chickens |
| CN113993242A (en) * | 2021-11-05 | 2022-01-28 | 南京农业大学 | Intelligent in-egg illumination regulation and control equipment and method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191836A (en) * | 2015-08-21 | 2015-12-30 | 中国海洋大学 | Scallop heartbeat index based rapid seed selection method |
| CN105165660A (en) * | 2015-10-20 | 2015-12-23 | 山东大学齐鲁医院 | Incubated living body egg embryo fetal monitoring device |
| CN105165660B (en) * | 2015-10-20 | 2017-08-25 | 山东大学齐鲁医院 | The monitoring device of live body poultry egg embryo movement of the foetus in hatching |
| RU2665117C2 (en) * | 2016-04-18 | 2018-08-28 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "РОССИЙСКИЙ ГОСУДАРСТВЕННЫЙ АГРАРНЫЙ ЗАОЧНЫЙ УНИВЕРСИТЕТ" | Method for recording the heart rate of a bird embryo without the shell destruction and the device for its implementation |
| CN108986066A (en) * | 2018-04-23 | 2018-12-11 | 中国农业科学院特产研究所 | A kind of detection method and system of chicken embryo |
| CN108990902A (en) * | 2018-09-03 | 2018-12-14 | 薄文海 | A kind of method for breeding improving pigeon eggs hatching rate |
| CN109819941A (en) * | 2019-04-04 | 2019-05-31 | 中国农业大学 | It is a kind of conducive to hatching and its later period chicken group healthy growth hatching illumination method |
| CN113575467A (en) * | 2021-07-29 | 2021-11-02 | 福州木鸡郎智能科技有限公司 | Block chain algorithm-based egg positioning method suitable for free-range chickens |
| CN113575467B (en) * | 2021-07-29 | 2022-09-09 | 福州木鸡郎智能科技有限公司 | Block chain algorithm-based egg positioning method suitable for free-range chickens |
| CN113993242A (en) * | 2021-11-05 | 2022-01-28 | 南京农业大学 | Intelligent in-egg illumination regulation and control equipment and method |
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