CN104304018A - Intermediate propagation method of lantern plant calyx tissue culture - Google Patents
Intermediate propagation method of lantern plant calyx tissue culture Download PDFInfo
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- CN104304018A CN104304018A CN201410550679.1A CN201410550679A CN104304018A CN 104304018 A CN104304018 A CN 104304018A CN 201410550679 A CN201410550679 A CN 201410550679A CN 104304018 A CN104304018 A CN 104304018A
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- fruit
- persistent calyx
- franchet groundcherry
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- groundcherry persistent
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Abstract
The invention discloses an intermediate propagation method of lantern plant calyx tissue culture. The method comprises the steps of sterilizing seeds, acquiring aseptic seedlings, proliferating the aseptic seedlings, exercising the seedlings, transplanting the seedlings and the like. A great amount of lantern plant calyx plants can be acquired within a short term, the genetic stability is good, simplicity in operation is realized, the sustainable growth of the lantern plant calyx seed resource can be facilitated, and the lantern plant calyx seed resource can be adequately utilized.
Description
Technical field
The present invention relates to the quick-breeding method of Plant Tissue Breeding, belong to plant technology field.
Background technology
Franchet groundcherry persistent calyx or fruit, Solanaceae, herbaceos perennial, high 1-2.5 chi, the root-like stock that ground bottom rail is walked, wildness is extremely strong, cold-resistant, disease-resistant, leaf alternate, blade is avette, edge in there being sparse to incise or wavy, spend singly be born in axil, white flowers, sagging, corolla is bell, fruit be berry, spherical, ripe time be orange red, suitable black loam, temperature all can grow between 5-35 DEG C, spring and summer at franchet groundcherry persistent calyx or fruit florescence, fruit autumn ripe, place calyx take on a red color or orange red time gather, dry, sweet, the micro-acid of fruit taste.Cold in nature, bitter, franchet groundcherry persistent calyx or fruit is China's distinctive medicine-food two-purpose health perennial herb wild fruit in history, and originate in China, north and south all has wild resource to distribute.Franchet groundcherry persistent calyx or fruit is a kind of medicinal plant, containing this sterol of standing grain, obtusifoliol, cycloartanol, ring jackfruit alkene etc. in seed, containing Charantin, has good result for the treatment of to diabetes; Root, stem, leaf, flower, fruit are all medicinal materials, and cauline leaf mashes rear external application can treat blister sore; Franchet groundcherry persistent calyx or fruit fruit also can be made into beverage, fruit wine, can, and find a good market with the effect of attacking of its bitter refreshing vermachen people health care, red sepal can extract pigment, also can be processed into tea, is desirable green drink.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for quickly breeding of franchet groundcherry persistent calyx or fruit tissue cultures, the present invention can obtain a large amount of franchet groundcherry persistent calyx or fruit plant in a short time, and genetic stability is good, simple to operate, contribute to the sustainable growth solving franchet groundcherry persistent calyx or fruit germ plasm resource, and make it be fully used.
Technical problem to be solved by this invention is realized by following scheme:
Get the seed of franchet groundcherry persistent calyx or fruit maturation, 35min is soaked in the liquid detergent of bacteriostatic agent, running water 1h, the concentrated sulfuric acid soaks 10min, running water is clean, 75% alcohol disinfecting 5min on superclean bench, 5% hydrogen peroxide treatment 20min, aseptic water washing 5 times, the franchet groundcherry persistent calyx or fruit seed access medium of disinfecting is the induction carrying out aseptic seedling in the medium of MS+NAA0.01mg/L, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the propagation of aseptic seedling is carried out in the aseptic seedling access T+TDZ4.0-5.0mg/L+NAA0.03-0.05mg/l+ inositol 30-50mg/l medium derived, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the blake bottle that test-tube plantlet after cultivating 50d is closed is opened, make tender seedling and extraneous contact, then greenhouse about 2d is placed in, progressively improve adaptive capacity, during transplanting, first lightly test-tube plantlet is taken out from blake bottle with tweezers, with flowing water, the solid culture medium that plant root is residual is rinsed well, then be transplanted to and fill in the flowerpot of garden mould, flowerpot is placed in the indoor without direct light, and humidity will remain on 40%.
The franchet groundcherry persistent calyx or fruit survival rate adopting the present invention to prepare is high, and cultivation cycle is short, and output is large, and less energy consumption, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Get the seed of franchet groundcherry persistent calyx or fruit maturation, 35min is soaked in the liquid detergent of bacteriostatic agent, running water 1h, the concentrated sulfuric acid soaks 10min, running water is clean, 75% alcohol disinfecting 5min on superclean bench, 5% hydrogen peroxide treatment 20min, aseptic water washing 5 times, the franchet groundcherry persistent calyx or fruit seed access medium of disinfecting is the induction carrying out aseptic seedling in the medium of MS+NAA0.01mg/L, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the propagation of aseptic seedling is carried out in the aseptic seedling access T+TDZ4.0mg/L+NAA0.03mg/l+ inositol 30mg/l medium derived, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the blake bottle that test-tube plantlet after cultivating 50d is closed is opened, make tender seedling and extraneous contact, then greenhouse about 2d is placed in, progressively improve adaptive capacity, during transplanting, first lightly test-tube plantlet is taken out from blake bottle with tweezers, with flowing water, the solid culture medium that plant root is residual is rinsed well, then be transplanted to and fill in the flowerpot of garden mould, flowerpot is placed in the indoor without direct light, and humidity will remain on 40%, survival rate 90%.
Embodiment 2
Get the seed of franchet groundcherry persistent calyx or fruit maturation, 35min is soaked in the liquid detergent of bacteriostatic agent, running water 1h, the concentrated sulfuric acid soaks 10min, running water is clean, 75% alcohol disinfecting 5min on superclean bench, 5% hydrogen peroxide treatment 20min, aseptic water washing 5 times, the franchet groundcherry persistent calyx or fruit seed access medium of disinfecting is the induction carrying out aseptic seedling in the medium of MS+NAA0.01mg/L, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the propagation of aseptic seedling is carried out in the aseptic seedling access T+TDZ5.0mg/L+NAA0.05mg/l+ inositol 50mg/l medium derived, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the blake bottle that test-tube plantlet after cultivating 50d is closed is opened, make tender seedling and extraneous contact, then greenhouse about 2d is placed in, progressively improve adaptive capacity, during transplanting, first lightly test-tube plantlet is taken out from blake bottle with tweezers, with flowing water, the solid culture medium that plant root is residual is rinsed well, then be transplanted to and fill in the flowerpot of garden mould, flowerpot is placed in the indoor without direct light, and humidity will remain on 40%, survival rate 91%.
Embodiment 3
Get the seed of franchet groundcherry persistent calyx or fruit maturation, 35min is soaked in the liquid detergent of bacteriostatic agent, running water 1h, the concentrated sulfuric acid soaks 10min, running water is clean, 75% alcohol disinfecting 5min on superclean bench, 5% hydrogen peroxide treatment 20min, aseptic water washing 5 times, the franchet groundcherry persistent calyx or fruit seed access medium of disinfecting is the induction carrying out aseptic seedling in the medium of MS+NAA0.01mg/L, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the propagation of aseptic seedling is carried out in the aseptic seedling access T+TDZ4.0mg/L+NAA0.04mg/l+ inositol 40mg/l medium derived, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C, the blake bottle that test-tube plantlet after cultivating 50d is closed is opened, make tender seedling and extraneous contact, then greenhouse about 2d is placed in, progressively improve adaptive capacity, during transplanting, first lightly test-tube plantlet is taken out from blake bottle with tweezers, with flowing water, the solid culture medium that plant root is residual is rinsed well, then be transplanted to and fill in the flowerpot of garden mould, flowerpot is placed in the indoor without direct light, and humidity will remain on 40%, survival rate 92%.
Claims (3)
1. a method for quickly breeding for franchet groundcherry persistent calyx or fruit tissue cultures, comprise the sterilization of seed, the acquisition of aseptic seedling, the propagation of aseptic seedling, its key step of acclimatization and transplants is as follows:
(1) seed of franchet groundcherry persistent calyx or fruit maturation is got, to its disinfection;
(2) the franchet groundcherry persistent calyx or fruit seed access medium of step (1) being disinfected is the induction carrying out aseptic seedling in the medium of MS+NAA0.01mg/L, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C;
(3) propagation of carrying out aseptic seedling in the aseptic seedling access T+TDZ 4.0-5.0mg/L+NAA 0.03-0.05mg/l+ inositol 30-50mg/l medium that step (2) derives is got, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 1500lx, photophase 12h/d, temperature 23 ± 1 DEG C;
(4) test-tube plantlet got after step (3) propagation carries out field transplanting.
2. according to the method for quickly breeding of a kind of franchet groundcherry persistent calyx or fruit tissue cultures according to claim 1, it is characterized in that: the acquisition of franchet groundcherry persistent calyx or fruit aseptic seed described in step (1) is, get the seed of franchet groundcherry persistent calyx or fruit maturation, in the liquid detergent of bacteriostatic agent, soak 35min, running water 1h, the concentrated sulfuric acid soaks 10min, running water is clean, 75% alcohol disinfecting 5min, 5% hydrogen peroxide treatment 20min on superclean bench, aseptic water washing 5 times.
3. according to the method for quickly breeding of a kind of franchet groundcherry persistent calyx or fruit tissue cultures according to claim 1, it is characterized in that: the method for transplanting in step (4) is opened by the blake bottle that the test-tube plantlet after cultivating 50d is closed, make tender seedling and extraneous contact, then greenhouse about 2d is placed in, progressively improve adaptive capacity, during transplanting, first lightly test-tube plantlet is taken out from blake bottle with tweezers, with flowing water, the solid culture medium that plant root is residual is rinsed well, then be transplanted to and fill in the flowerpot of garden mould, flowerpot is placed in the indoor without direct light, and humidity to remain on 40%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410550679.1A CN104304018A (en) | 2014-10-17 | 2014-10-17 | Intermediate propagation method of lantern plant calyx tissue culture |
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| CN201410550679.1A CN104304018A (en) | 2014-10-17 | 2014-10-17 | Intermediate propagation method of lantern plant calyx tissue culture |
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| CN104304018A true CN104304018A (en) | 2015-01-28 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766646A (en) * | 2016-03-31 | 2016-07-20 | 内蒙古自治区农牧业科学院 | Tissue culture method for physochlaina |
| CN106538387A (en) * | 2016-10-31 | 2017-03-29 | 杭州师范大学 | A kind of method for tissue culture of Herba Physalis pubescentis |
| CN106718897A (en) * | 2016-12-08 | 2017-05-31 | 黑龙江省林业科学研究所 | A kind of culture medium and cultural method of induction wintercherry plant regeneration |
| CN110710451A (en) * | 2019-08-03 | 2020-01-21 | 周口师范学院 | Physalis alkekengi root tuber induction generation method and root tuber-like body-mediated genetic transformation method |
-
2014
- 2014-10-17 CN CN201410550679.1A patent/CN104304018A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766646A (en) * | 2016-03-31 | 2016-07-20 | 内蒙古自治区农牧业科学院 | Tissue culture method for physochlaina |
| CN106538387A (en) * | 2016-10-31 | 2017-03-29 | 杭州师范大学 | A kind of method for tissue culture of Herba Physalis pubescentis |
| CN106538387B (en) * | 2016-10-31 | 2018-09-07 | 杭州师范大学 | A kind of method for tissue culture of Ku Zhi |
| CN106718897A (en) * | 2016-12-08 | 2017-05-31 | 黑龙江省林业科学研究所 | A kind of culture medium and cultural method of induction wintercherry plant regeneration |
| CN106718897B (en) * | 2016-12-08 | 2019-04-16 | 黑龙江省林业科学研究所 | A kind of culture medium and cultural method inducing wintercherry plant regeneration |
| CN110710451A (en) * | 2019-08-03 | 2020-01-21 | 周口师范学院 | Physalis alkekengi root tuber induction generation method and root tuber-like body-mediated genetic transformation method |
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Application publication date: 20150128 |