CN104293963A - Method for identifying inbred line of Gottingen strain by use of 145 SNPs - Google Patents

Method for identifying inbred line of Gottingen strain by use of 145 SNPs Download PDF

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CN104293963A
CN104293963A CN201410562272.0A CN201410562272A CN104293963A CN 104293963 A CN104293963 A CN 104293963A CN 201410562272 A CN201410562272 A CN 201410562272A CN 104293963 A CN104293963 A CN 104293963A
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snp site
snp
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pig
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CN104293963B (en
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冯书堂
牟玉莲
韩建林
李奎
高倩
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Beijing Grand Life Science and Technology Co., Ltd.
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Abstract

The invention discloses a method for identifying an inbred line of Gottingen strain. A method for assisting to identify whether a pig to be tested is of an inbred line of Gottingen strain comprises the following steps: detecting the genotype of the pig to be tested based on 145 SNP sites; if the genotype satisfies all the standards of (1) to (145), identifying that the pig to be tested is a candidate inbred line of Gottingen strain; if the genotype does not satisfy all the standards of (1) to (145), identifying that the pig to be tested is a candidate non-inbred line of Gottingen strain. The method for identifying the inbred line of Gottingen strain has an enormous value for the germplasm resource identification of the inbred line of Gottingen strain.

Description

The method of a kind of application 145 SNP judgment of Wuzhishan minipig inbred line
Technical field
The present invention relates to the method for a kind of application 145 SNP judgment of Wuzhishan minipig inbred line.
Background technology
During 1989-2003 years, " Wuzhi Mountain inbred cultivation, Specific character and exploitation " in the Ministry of Agriculture's " the Seventh Five-Year Plan, eight or five, 95 " industry brainstorm project, and the Department of Science and Technology " 863 ", National Nature fund committee emphasis, under the subsidy of major project about 7 problems such as grade, research work makes a breakthrough, that is: with 1 male 1 female Wuzhi Mountain pig (also known as WZSP) for being ancestral, continuous employing " son joins mother ", the aggregate measures such as " full sibs " mating, progressively overcome inbreeding to breed and cause reproductive survival rate extremely low, less than 20% detrimentally affect, set up F 17inbred lines colony and pedigree, and carried out utilizing the technique means of the multiple advanced persons such as molecular genetic means DNA fingerprint figure similarity factor, micro-satellite to monitor this inbred lines kind F 7-F 16inbred cultivation research process, preliminary genetic development and the feature disclosing Inbreding Wuzhishan pig, demonstrates the verity of this inbred cultivation process, science.And carried out a large amount of exploitation research, demonstrate the type that is unsurpassed in excellence as ideals of human being Research of Animal Model for Study, obtain certain economic benefit and larger social benefit.Its research contents is identified by Ministry of Agriculture's technological achievement respectively at nineteen ninety-five and 2005 " WZSP Specific character and exploitation research " (No. [95] the 075th, agriculture section fruit qualification), " WZSP experiment inbred cultivation and Molecular and genetic basis are studied " two achievements, wherein within 1999, obtains Ministry of Agriculture's scientific-technical progress third prize.
Miniature pig inbreeding is cultivated and is is tentatively researched and developed achievement, creates larger impact at home and abroad, and e.g., the U.S., Italy, Japan, West Germany, Korea S etc. require to introduce a fine variety or joint study one after another, and especially the U.S. proposes 5,000,000 dollars of 50 boars bought at that time for 1996; Korea S does not agree to introduce a fine variety latter 2000 and proposes investment 5,000,000 yuan China is joint and does factory etc.Its major cause is that inbred lines animal is special Animal Genetic Resource, it can be the same with precision instrument physically to chemical analysis pure reagent, can draw rapidly for life science, sensitive, science data accurately, there is very high use value and Research Significance, so cultivated more than 450 kind of inbred lines system mouse, rat etc. in the world at present.Small-sized mammalian inbred lines, as animal model, has been widely used in solving mankind's difficult and complicated illness, capturing life science and the research field such as medical science, a pharmacy difficult problem.Due to the difference in many ways such as physiology between planting, inbred pig is cultivated very difficult, has no reported success in the world so far through looking into new confirmation (2013.11).But pig is compared with muroid has larger similarity with the mankind, solving mankind's difficult and complicated illness, capturing in life science and the research such as medical science, a pharmacy difficult problem and will play replaceable, not unique effect.Inbred pig is macrofauna gene genetic resource rare, valuable in the world.Before this, far away from the sixties in last century USA and Europe many units of some developed countries, just go out huge fund and carried out inbred pig nurturing research, but fail, inbreeding coefficient is the highest only can reach 0.75.And China was by 15 years nurturing researchs, the miniature pig resource utilizing China special obtains F 17, inbreeding coefficient reaches 0.965, more the good news is overcome, cross over close breeding cause stage occurred frequently of piglet death.Therefore, inbred pig is cultivated and successfully probably to be realized first in China.Through further work in recent years, obtain Inbreding Wuzhishan pig F now 17to F 22.
Because not yet there is cultivation reported success the inbred pig world, more building without inbred lines is standard and authentication method.Therefore, must use for reference, strictly follow inbred mouse, the method for cultivation of rat carries out, namely same ancestral 2 pig close relatives all one's life breed 20 generations more than and set up complete pedigree, detect its genetic stability, set up authentication method, set up strain standard especially and between existing Hainan Mt. Wu-zhi Shan pig kind to distinguish, science, credible evidence could be provided to common people, just can complete the unprecedented rear temporary genetic resources Innovation project without the person that has come, promote the well-being of mankind.
Once the success of miniature pig inbred cultivation, China will have independent intellectual property right, have the pig genetic germplasm resource of the innovation of the leading level in the world, will fill up world research in this research field blank, and enrich macrofauna inbred cultivation theory and practice; As " for the difficult person " of ideals of human being, the important foundation stone that makes progress of biotechnology research and basis for innovation, greatly will promote cooperative development and the innovation of the aspects such as China's life science, physianthropy, pharmacy, for solving mankind difficult and complicated illness, extending life and promote the well-being of mankind and make irreplaceable importance and functions.Therefore, to have important practical significance and theory significance.
Summary of the invention
The object of this invention is to provide the method for a kind of application 145 SNP judgment of Wuzhishan minipig inbred line.
The invention provides the method whether a kind of assistant identification pig to be measured is Inbreding Wuzhishan pig, comprise the steps:
Detect the genotype of pig to be measured based on 145 SNP site;
If meet all standards of following (1) to (145), pig to be measured is the Inbreding Wuzhishan pig of candidate:
If do not meet all standards of above-mentioned (1) to (145), pig to be measured is the non-Inbreding Wuzhishan pig of candidate.
Described pig to be measured can be Inbreding Wuzhishan pig or Hainan Mt. Wu-zhi Shan pig.
Described Inbreding Wuzhishan pig can belong to F 20to F 22in arbitrary generation.
Described Inbreding Wuzhishan pig can belong to F 20generation or F 20generation later from generation to generation.
In described method, certain chip can be adopted to detect the genotype of pig to be measured based on 145 SNP site; Described certain chip for be fixed with sequence table respectively at difference sequence 1 to sequence 145 shown in the nucleic acid chip of single strand dna.
The present invention also protects a kind of nucleic acid chip, is fixed with the single strand dna shown in sequence 1 to sequence 145 of sequence table respectively at difference.
The present invention also protects the application of described nucleic acid chip, is following (a) or (b): whether (a) assistant identification pig to be measured is Inbreding Wuzhishan pig; Whether (b) assistant identification swinery body to be measured is Inbreding Wuzhishan pig colony.
Described pig to be measured can be Inbreding Wuzhishan pig or Hainan Mt. Wu-zhi Shan pig.
Described Inbreding Wuzhishan pig can belong to F 20to F 22in arbitrary generation.
Described Inbreding Wuzhishan pig can belong to F 20generation or F 20generation later from generation to generation.
Present invention also offers the method whether a kind of assistant identification swinery body to be measured is Inbreding Wuzhishan pig colony, comprise the steps:
From swinery body to be measured, stochastic sampling obtains sample to be tested, then the genotype of each sample to be tested based on 145 SNP site is detected, if all samples to be tested all meet all standards of following (1) to (145), swinery body to be measured is the Inbreding Wuzhishan pig colony of candidate:
If there are all standards that more than does not meet above-mentioned (1) to (145) in sample to be tested, swinery body to be measured is the non-Inbreding Wuzhishan pig colony of candidate.
In described method, from swinery body to be measured, stochastic sampling obtains the sample to be tested of statistical significance.
In described method, from swinery body to be measured, stochastic sampling obtains more than 16 samples to be tested.
Described pig to be measured can be Inbreding Wuzhishan pig or Hainan Mt. Wu-zhi Shan pig.
Described Inbreding Wuzhishan pig can belong to F 20to F 22in arbitrary generation.
Described Inbreding Wuzhishan pig can belong to F 20generation or F 20generation later from generation to generation.
In described method, certain chip can be adopted to detect the genotype of pig to be measured based on 145 SNP site; Described certain chip for be fixed with sequence table respectively at difference sequence 1 to sequence 145 shown in the nucleic acid chip of single strand dna.
The present invention has substantial worth for the germplasm identification of Inbreding Wuzhishan pig.
Embodiment
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.Test materials used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment for three times, results averaged.
Inbreding Wuzhishan pig:
Li Kai, Feng Shutang, Mu Yulian, Yang Shulin, Han Jianlin, Liu Lan, the new rising sun of member, Guo Yong, WZSP 3 inbreeding family microsatellite allele heredity changes, Scientia Agricultura Sinica, 2012,42 (5): 1751-1760.
Hainan Mt. Wu-zhi Shan pig:
Hou Guan Yu, Wang Dongjing, Guan Song, glorious, Huang Xianzhou, Wuzhi Mountain pig microcommunity genetics detects, ecology of domestic animals, the 2007,28th volume the 6th phase: 44-47.
The laboratory animal of Inbreding Wuzhishan pig is 48, wherein F 20for 16, F 21for 16, F 22generation 16.The laboratory animal of Hainan Mt. Wu-zhi Shan pig is 16.
The foundation of embodiment 1, method
, there are 145 SNP site, according to its genotype, Inbreding Wuzhishan pig and non-Inbreding Wuzhishan pig can be differentiated in the present inventor's discovery when carrying out genome research to Inbreding Wuzhishan pig and non-Inbreding Wuzhishan pig.The genotype of 145 SNP site of Inbreding Wuzhishan pig is homozygous (genotype described in table 1).145 SNP site of non-Inbreding Wuzhishan pig are heterozygous.
In 145 SNP site, 12 are positioned at No. 1 chromosomal 1st section, 16 are positioned at No. 1 chromosomal 15th section, 14 and are positioned at No. 1 chromosomal 50th section, 16 are positioned at No. 1 chromosomal 78th section, and 15 are positioned at No. 2 chromosomal 2nd section, and 14 are positioned at No. 2 chromosomal 12nd section, 13 are positioned at No. 2 chromosomal 19th section, 20 are positioned at No. 18 chromosomal 5th section, and 13 are positioned at No. 18 chromosomal 14th section, and 12 are positioned at No. 18 chromosomal 20th section.
(calmodulin binding domain CaM and the SNP site of probe and genomic dna are adjacent to devise 145 probes based on 145 SNP site the present inventor, be positioned at upstream or the downstream of SNP site, therefore extended by end, the genotype of SNP site can be known).
The source sequence residing on genomic dna of the genotype of the title of 145 SNP, residing karyomit(e), position on chromosome, Inbreding Wuzhishan pig, the Source Type of SNP, the nucleotide sequence of 145 probes and SNP is in table 1.
Table 1
In table 1, N represents A, T, C or G.In table 1, the region (containing two kinds of forms, probe is identical with the nucleotide sequence of underlined region, the nucleotide sequence reverse complemental of probe and underlined region) that the underscore mark probe in source sequence is corresponding.In table 1, when the Source Type of SNP is [T/G], heterozygous is TG, and when the Source Type of SNP is [A/G], heterozygous is AG, and when the Source Type of SNP is [T/C], heterozygous is TC, and when the Source Type of SNP is [A/C], heterozygous is AC.
Embodiment 2, application 145 SNP judgment of Wuzhishan minipig inbred line and non-Inbreding Wuzhishan pig
1, get laboratory animal, ear vein gets blood, from blood sample, extract genomic dna.
2, get the genomic dna that step 1 obtains, hybridize with the nucleic acid chip being fixed with 145 probes (145 probes are respectively the single strand dna shown in the sequence 145 of sequence 1 to the sequence table of sequence table).
3, after completing steps 2, each point in nucleic acid chip is carried out end extension, thus knows the genotype of 145 SNP site in genomic dna.
4, get the genomic dna that step 1 obtains, carry out genome sequencing, know the genotype of 145 SNP site in genomic dna.
Result shows, the result of step 3 and the result of step 4 completely the same.
The genotype of 145 SNP site of 48 laboratory animal of Inbreding Wuzhishan pig is homozygous (genotype described in the table 1 of embodiment 1).145 SNP site of 16 laboratory animal of Hainan Mt. Wu-zhi Shan pig are heterozygous.

Claims (10)

1. whether assistant identification pig to be measured is a method for Inbreding Wuzhishan pig, comprises the steps:
Detect the genotype of pig to be measured based on 145 SNP site;
If meet all standards of following (1) to (145), pig to be measured is the Inbreding Wuzhishan pig of candidate:
(1) genotype of M1GA0025062 SNP site is GG;
(2) genotype of DRGA0017627 SNP site is GG;
(3) genotype of H3GA0056137 SNP site is CC;
(4) genotype of MARC0039661 SNP site is AA;
(5) genotype of ASGA0085025 SNP site is TT;
(6) genotype of ASGA0098570 SNP site is GG;
(7) genotype of MARC0063358 SNP site is AA;
(8) genotype of ASGA0085437 SNP site is GG;
(9) genotype of MARC0096709 SNP site is CC;
(10) genotype of H3GA0052387 SNP site is GG;
(11) genotype of ASGA0000264 SNP site is TT;
(12) genotype of ALGA0000195 SNP site is TT;
(13) genotype of H3GA0001444 SNP site is AA;
(14) genotype of ASGA0002293 SNP site is CC;
(15) genotype of INRA0001752 SNP site is GG;
(16) genotype of H3GA0001445 SNP site is GG;
(17) genotype of ALGA0002595 SNP site is TT;
(18) genotype of ALGA0002600 SNP site is GG;
(19) genotype of DRGA0000563 SNP site is CC;
(20) genotype of DRGA0000565 SNP site is CC;
(21) genotype of INRA0001761 SNP site is AA;
(22) genotype of MARC0104045 SNP site is CC;
(23) genotype of ALGA0002601 SNP site is CC;
(24) genotype of DRGA0000569 SNP site is AA;
(25) genotype of ALGA0002608 SNP site is TT;
(26) genotype of DRGA0000568 SNP site is CC;
(27) genotype of ALGA0002604 SNP site is GG;
(28) genotype of MARC0048118 SNP site is TT;
(29) genotype of ASGA0004229 SNP site is CC;
(30) genotype of MARC0007088 SNP site is CC;
(31) genotype of MARC0000061 SNP site is GG;
(32) genotype of MARC0094747 SNP site is AA;
(33) genotype of MARC0007969 SNP site is AA;
(34) genotype of ASGA0106092 SNP site is CC;
(35) genotype of ALGA0119806 SNP site is GG;
(36) genotype of INRA0003745 SNP site is AA;
(37) genotype of INRA0003746 SNP site is TT;
(38) genotype of ALGA0005490 SNP site is CC;
(39) genotype of H3GA0002538 SNP site is CC;
(40) genotype of INRA0003749 SNP site is CC;
(41) genotype of H3GA0002539 SNP site is GG;
(42) genotype of M1GA0001099 SNP site is CC;
(43) genotype of MARC0111831 SNP site is AA;
(44) genotype of ASGA0005566 SNP site is CC;
(45) genotype of ALGA0007578 SNP site is CC;
(46) genotype of ASGA0005568 SNP site is CC;
(47) genotype of INRA0005765 SNP site is AA;
(48) genotype of MARC0095347 SNP site is CC;
(49) genotype of MARC0039015 SNP site is CC;
(50) genotype of DRGA0001892 SNP site is GG;
(51) genotype of ALGA0007580 SNP site is GG;
(52) genotype of ALGA0007583 SNP site is TT;
(53) genotype of MARC0095915 SNP site is CC;
(54) genotype of ASGA0005571 SNP site is AA;
(55) genotype of MARC0014134 SNP site is AA;
(56) genotype of MARC0024233 SNP site is AA;
(57) genotype of H3GA0003548 SNP site is GG;
(58) genotype of ALGA0007591 SNP site is CC;
(59) genotype of MARC0000195 SNP site is CC;
(60) genotype of ALGA0038697 SNP site is CC;
(61) genotype of H3GA0020000 SNP site is CC;
(62) genotype of ALGA0038703 SNP site is CC;
(63) genotype of H3GA0020002 SNP site is GG;
(64) genotype of DRGA0007138 SNP site is CC;
(65) genotype of ASGA0031284 SNP site is CC;
(66) genotype of ASGA0031285 SNP site is AA;
(67) genotype of MARC0025042 SNP site is GG;
(68) genotype of H3GA0020006 SNP site is TT;
(69) genotype of ALGA0038729 SNP site is CC;
(70) genotype of ALGA0038731 SNP site is GG;
(71) genotype of ASGA0031321 SNP site is GG;
(72) genotype of ASGA0031322 SNP site is GG;
(73) genotype of ALGA0038747 SNP site is AA;
(74) genotype of ASGA0033095 SNP site is TT;
(75) genotype of ALGA0040854 SNP site is GG;
(76) genotype of ALGA0040856 SNP site is AA;
(77) genotype of ALGA0040857 SNP site is GG;
(78) genotype of ASGA0033096 SNP site is GG;
(79) genotype of INRA0025180 SNP site is AA;
(80) genotype of ALGA0040859 SNP site is CC;
(81) genotype of H3GA0021216 SNP site is CC;
(82) genotype of ASGA0033098 SNP site is CC;
(83) genotype of H3GA0021221 SNP site is CC;
(84) genotype of MARC0014540 SNP site is CC;
(85) genotype of ASGA0033103 SNP site is CC;
(86) genotype of DIAS0000557 SNP site is AA;
(87) genotype of ASGA0033116 SNP site is AA;
(88) genotype of ASGA0036835 SNP site is CC;
(89) genotype of MARC0031932 SNP site is CC;
(90) genotype of ASGA0036838 SNP site is CC;
(91) genotype of H3GA0023523 SNP site is GG;
(92) genotype of ALGA0045460 SNP site is TT;
(93) genotype of MARC0005927 SNP site is GG;
(94) genotype of MARC0005928 SNP site is AA;
(95) genotype of ASGA0036842 SNP site is CC;
(96) genotype of ASGA0036846 SNP site is CC;
(97) genotype of MARC0098637 SNP site is TT;
(98) genotype of M1GA0011035 SNP site is TT;
(99) genotype of DRGA0008230 SNP site is GG;
(100) genotype of ASGA0036855 SNP site is CC;
(101) genotype of ALGA0097277 SNP site is GG;
(102) genotype of MARC0080197 SNP site is CC;
(103) genotype of H3GA0050489 SNP site is TT;
(104) genotype of ALGA0097281 SNP site is TT;
(105) genotype of H3GA0050490 SNP site is CC;
(106) genotype of ALGA0097282 SNP site is TT;
(107) genotype of ASGA0079089 SNP site is CC;
(108) genotype of INRA0055354 SNP site is GG;
(109) genotype of ASGA0079091 SNP site is TT;
(110) genotype of ASGA0079090 SNP site is CC;
(111) genotype of H3GA0050491 SNP site is AA;
(112) genotype of MARC0056017 SNP site is TT;
(113) genotype of MARC0055759 SNP site is AA;
(114) genotype of H3GA0050495 SNP site is GG;
(115) genotype of ALGA0097291 SNP site is GG;
(116) genotype of ALGA0097290 SNP site is GG;
(117) genotype of CASI0006683 SNP site is GG;
(118) genotype of ASGA0079098 SNP site is CC;
(119) genotype of ALGA0097297 SNP site is GG;
(120) genotype of H3GA0054426 SNP site is TT;
(121) genotype of ALGA0098112 SNP site is GG;
(122) genotype of MARC0089391 SNP site is GG;
(123) genotype of ASGA0089892 SNP site is AA;
(124) genotype of ASGA0097792 SNP site is CC;
(125) genotype of MARC0046857 SNP site is GG;
(126) genotype of MARC0077194 SNP site is AA;
(127) genotype of ALGA0108769 SNP site is CC;
(128) genotype of H3GA0050799 SNP site is CC;
(129) genotype of ALGA0098120 SNP site is CC;
(130) genotype of ALGA0098123 SNP site is GG;
(131) genotype of ALGA0098128 SNP site is GG;
(132) genotype of ASGA0079719 SNP site is CC;
(133) genotype of ASGA0079728 SNP site is GG;
(134) genotype of ASGA0080429 SNP site is CC;
(135) genotype of MARC0052755 SNP site is GG;
(136) genotype of ALGA0098918 SNP site is GG;
(137) genotype of ASGA0080432 SNP site is GG;
(138) genotype of ALGA0098922 SNP site is CC;
(139) genotype of INRA0056206 SNP site is GG;
(140) genotype of INRA0056207 SNP site is CC;
(141) genotype of ASGA0080435 SNP site is CC;
(142) genotype of MARC0068495 SNP site is GG;
(143) genotype of ASGA0080436 SNP site is CC;
(144) genotype of MARC0003370 SNP site is CC;
(145) genotype of ASGA0085659 SNP site is CC;
If do not meet all standards of above-mentioned (1) to (145), pig to be measured is the non-Inbreding Wuzhishan pig of candidate.
2. the method for claim 1, is characterized in that: described pig to be measured is Inbreding Wuzhishan pig or Hainan Mt. Wu-zhi Shan pig.
3. method as claimed in claim 1 or 2, is characterized in that: described Inbreding Wuzhishan pig belongs to F 20to F 22in arbitrary generation.
4. method as claimed in claim 1 or 2, is characterized in that: described Inbreding Wuzhishan pig belongs to F 20generation or F 20generation later from generation to generation.
5. as the method as described in arbitrary in Claims 1-4, it is characterized in that: in described method, adopt certain chip to detect the genotype of pig to be measured based on 145 SNP site; Described certain chip for be fixed with sequence table respectively at difference sequence 1 to sequence 145 shown in the nucleic acid chip of single strand dna.
6. a nucleic acid chip, is fixed with the single strand dna shown in sequence 1 to sequence 145 of sequence table respectively at difference.
7. the application of nucleic acid chip described in claim 6, is following (a) or (b):
Whether (a) assistant identification pig to be measured is Inbreding Wuzhishan pig;
Whether (b) assistant identification swinery body to be measured is Inbreding Wuzhishan pig colony.
8. whether assistant identification swinery body to be measured is a method for Inbreding Wuzhishan pig colony, comprises the steps:
From swinery body to be measured, stochastic sampling obtains sample to be tested, then the genotype of each sample to be tested based on 145 SNP site is detected, if all samples to be tested all meet all standards of following (1) to (145), swinery body to be measured is the Inbreding Wuzhishan pig colony of candidate:
(1) genotype of M1GA0025062 SNP site is GG;
(2) genotype of DRGA0017627 SNP site is GG;
(3) genotype of H3GA0056137 SNP site is CC;
(4) genotype of MARC0039661 SNP site is AA;
(5) genotype of ASGA0085025 SNP site is TT;
(6) genotype of ASGA0098570 SNP site is GG;
(7) genotype of MARC0063358 SNP site is AA;
(8) genotype of ASGA0085437 SNP site is GG;
(9) genotype of MARC0096709 SNP site is CC;
(10) genotype of H3GA0052387 SNP site is GG;
(11) genotype of ASGA0000264 SNP site is TT;
(12) genotype of ALGA0000195 SNP site is TT;
(13) genotype of H3GA0001444 SNP site is AA;
(14) genotype of ASGA0002293 SNP site is CC;
(15) genotype of INRA0001752 SNP site is GG;
(16) genotype of H3GA0001445 SNP site is GG;
(17) genotype of ALGA0002595 SNP site is TT;
(18) genotype of ALGA0002600 SNP site is GG;
(19) genotype of DRGA0000563 SNP site is CC;
(20) genotype of DRGA0000565 SNP site is CC;
(21) genotype of INRA0001761 SNP site is AA;
(22) genotype of MARC0104045 SNP site is CC;
(23) genotype of ALGA0002601 SNP site is CC;
(24) genotype of DRGA0000569 SNP site is AA;
(25) genotype of ALGA0002608 SNP site is TT;
(26) genotype of DRGA0000568 SNP site is CC;
(27) genotype of ALGA0002604 SNP site is GG;
(28) genotype of MARC0048118 SNP site is TT;
(29) genotype of ASGA0004229 SNP site is CC;
(30) genotype of MARC0007088 SNP site is CC;
(31) genotype of MARC0000061 SNP site is GG;
(32) genotype of MARC0094747 SNP site is AA;
(33) genotype of MARC0007969 SNP site is AA;
(34) genotype of ASGA0106092 SNP site is CC;
(35) genotype of ALGA0119806 SNP site is GG;
(36) genotype of INRA0003745 SNP site is AA;
(37) genotype of INRA0003746 SNP site is TT;
(38) genotype of ALGA0005490 SNP site is CC;
(39) genotype of H3GA0002538 SNP site is CC;
(40) genotype of INRA0003749 SNP site is CC;
(41) genotype of H3GA0002539 SNP site is GG;
(42) genotype of M1GA0001099 SNP site is CC;
(43) genotype of MARC0111831 SNP site is AA;
(44) genotype of ASGA0005566 SNP site is CC;
(45) genotype of ALGA0007578 SNP site is CC;
(46) genotype of ASGA0005568 SNP site is CC;
(47) genotype of INRA0005765 SNP site is AA;
(48) genotype of MARC0095347 SNP site is CC;
(49) genotype of MARC0039015 SNP site is CC;
(50) genotype of DRGA0001892 SNP site is GG;
(51) genotype of ALGA0007580 SNP site is GG;
(52) genotype of ALGA0007583 SNP site is TT;
(53) genotype of MARC0095915 SNP site is CC;
(54) genotype of ASGA0005571 SNP site is AA;
(55) genotype of MARC0014134 SNP site is AA;
(56) genotype of MARC0024233 SNP site is AA;
(57) genotype of H3GA0003548 SNP site is GG;
(58) genotype of ALGA0007591 SNP site is CC;
(59) genotype of MARC0000195 SNP site is CC;
(60) genotype of ALGA0038697 SNP site is CC;
(61) genotype of H3GA0020000 SNP site is CC;
(62) genotype of ALGA0038703 SNP site is CC;
(63) genotype of H3GA0020002 SNP site is GG;
(64) genotype of DRGA0007138 SNP site is CC;
(65) genotype of ASGA0031284 SNP site is CC;
(66) genotype of ASGA0031285 SNP site is AA;
(67) genotype of MARC0025042 SNP site is GG;
(68) genotype of H3GA0020006 SNP site is TT;
(69) genotype of ALGA0038729 SNP site is CC;
(70) genotype of ALGA0038731 SNP site is GG;
(71) genotype of ASGA0031321 SNP site is GG;
(72) genotype of ASGA0031322 SNP site is GG;
(73) genotype of ALGA0038747 SNP site is AA;
(74) genotype of ASGA0033095 SNP site is TT;
(75) genotype of ALGA0040854 SNP site is GG;
(76) genotype of ALGA0040856 SNP site is AA;
(77) genotype of ALGA0040857 SNP site is GG;
(78) genotype of ASGA0033096 SNP site is GG;
(79) genotype of INRA0025180 SNP site is AA;
(80) genotype of ALGA0040859 SNP site is CC;
(81) genotype of H3GA0021216 SNP site is CC;
(82) genotype of ASGA0033098 SNP site is CC;
(83) genotype of H3GA0021221 SNP site is CC;
(84) genotype of MARC0014540 SNP site is CC;
(85) genotype of ASGA0033103 SNP site is CC;
(86) genotype of DIAS0000557 SNP site is AA;
(87) genotype of ASGA0033116 SNP site is AA;
(88) genotype of ASGA0036835 SNP site is CC;
(89) genotype of MARC0031932 SNP site is CC;
(90) genotype of ASGA0036838 SNP site is CC;
(91) genotype of H3GA0023523 SNP site is GG;
(92) genotype of ALGA0045460 SNP site is TT;
(93) genotype of MARC0005927 SNP site is GG;
(94) genotype of MARC0005928 SNP site is AA;
(95) genotype of ASGA0036842 SNP site is CC;
(96) genotype of ASGA0036846 SNP site is CC;
(97) genotype of MARC0098637 SNP site is TT;
(98) genotype of M1GA0011035 SNP site is TT;
(99) genotype of DRGA0008230 SNP site is GG;
(100) genotype of ASGA0036855 SNP site is CC;
(101) genotype of ALGA0097277 SNP site is GG;
(102) genotype of MARC0080197 SNP site is CC;
(103) genotype of H3GA0050489 SNP site is TT;
(104) genotype of ALGA0097281 SNP site is TT;
(105) genotype of H3GA0050490 SNP site is CC;
(106) genotype of ALGA0097282 SNP site is TT;
(107) genotype of ASGA0079089 SNP site is CC;
(108) genotype of INRA0055354 SNP site is GG;
(109) genotype of ASGA0079091 SNP site is TT;
(110) genotype of ASGA0079090 SNP site is CC;
(111) genotype of H3GA0050491 SNP site is AA;
(112) genotype of MARC0056017 SNP site is TT;
(113) genotype of MARC0055759 SNP site is AA;
(114) genotype of H3GA0050495 SNP site is GG;
(115) genotype of ALGA0097291 SNP site is GG;
(116) genotype of ALGA0097290 SNP site is GG;
(117) genotype of CASI0006683 SNP site is GG;
(118) genotype of ASGA0079098 SNP site is CC;
(119) genotype of ALGA0097297 SNP site is GG;
(120) genotype of H3GA0054426 SNP site is TT;
(121) genotype of ALGA0098112 SNP site is GG;
(122) genotype of MARC0089391 SNP site is GG;
(123) genotype of ASGA0089892 SNP site is AA;
(124) genotype of ASGA0097792 SNP site is CC;
(125) genotype of MARC0046857 SNP site is GG;
(126) genotype of MARC0077194 SNP site is AA;
(127) genotype of ALGA0108769 SNP site is CC;
(128) genotype of H3GA0050799 SNP site is CC;
(129) genotype of ALGA0098120 SNP site is CC;
(130) genotype of ALGA0098123 SNP site is GG;
(131) genotype of ALGA0098128 SNP site is GG;
(132) genotype of ASGA0079719 SNP site is CC;
(133) genotype of ASGA0079728 SNP site is GG;
(134) genotype of ASGA0080429 SNP site is CC;
(135) genotype of MARC0052755 SNP site is GG;
(136) genotype of ALGA0098918 SNP site is GG;
(137) genotype of ASGA0080432 SNP site is GG;
(138) genotype of ALGA0098922 SNP site is CC;
(139) genotype of INRA0056206 SNP site is GG;
(140) genotype of INRA0056207 SNP site is CC;
(141) genotype of ASGA0080435 SNP site is CC;
(142) genotype of MARC0068495 SNP site is GG;
(143) genotype of ASGA0080436 SNP site is CC;
(144) genotype of MARC0003370 SNP site is CC;
(145) genotype of ASGA0085659 SNP site is CC;
If there are all standards that more than does not meet above-mentioned (1) to (145) in sample to be tested, swinery body to be measured is the non-Inbreding Wuzhishan pig colony of candidate.
9. method as claimed in claim 8, is characterized in that: described pig to be measured is Inbreding Wuzhishan pig or Hainan Mt. Wu-zhi Shan pig.
10. method as claimed in claim 8 or 9, is characterized in that: in described method, adopts certain chip to detect the genotype of each sample to be tested based on 145 SNP site; Described certain chip for be fixed with sequence table respectively at difference sequence 1 to sequence 145 shown in the nucleic acid chip of single strand dna.
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