CN104293517B - A kind of coating type enzyme granulate and preparation method thereof - Google Patents
A kind of coating type enzyme granulate and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of coating type enzyme granulate and preparation method thereof, specifically, the present invention provides a kind of coating type enzyme granulate, including enzyme ball and coating, the enzyme ball contains enzyme component and stabilizer.The present invention also provides the preparation methods of above-mentioned coating type enzyme granulate.Technical solution provided by the invention, enzyme granulate have that preferable mixture homogeneity, enzyme granulate disintegration time is short, the holding time is long with washing powder, and the coating type enzyme granulate homogeneity of preparation is good, stable quality.
Description
Technical field
The invention belongs to enzyme engineering field, specifically, the present invention relates to a kind of washing enzyme granulation preparations.
Background technology
Washing is the widely used enzyme preparation of existing market with enzyme, special since it can increase substantially washing dirt-removing power
It is other to protides dirts such as bloodstain, sweat stain, milk stain, oil stains, there is unique washing effect.Conventional washing is industrially with solid
Particle shape formula is added in washing powder, and dirt-removing power is washed, but dust, vehicle are easily generated when processing prepares enzyme granulate to enhance it
Between residual dust can cause the allergic reaction of contact to part population, such as long-term excessive contact can cause can be to human body skin
Skin, eyes and schneiderian membrane membrane tissue damage.The washing of existing coating type is with enzyme generally existing is expensive, particle homogeneity
Difference, the problems such as disintegration rate is low.
Novozymes Company of Denmark and company of American Energy Saving section do a lot of work in terms of washing enzyme, domestic and international mainstream
Prilling is mainly the equipment such as centrifugal granulator combination fluid bed, although the technique can obtain that disintegrating property makes moderate progress
Grain, but centrifugal granulator and fluid unit are expensive, it is high that craftsmanship is required, product yield is relatively low and product particle is equal
Evenness is poor.In addition centrifugal granulating and fluid bed generate a large amount of enzyme powders in preparation process is processed, and workshop residual dust is to part
Crowd can cause the allergic reaction of contact, and such as long-term excessive contact can cause can be to human skin, eyes and schneiderian membrane film
Tissue damages.
Those skilled in the art are dedicated to developing a kind of preparation process of new coating type enzyme granulate.So as to safety,
It is easy, efficient to prepare coating type washing enzyme, it realizes self raising flour dirt or is granulated without dust, improve the stability of production technology,
Reduce influence of the human factor to product quality and yield and realize granzyme and washing powder have preferable mixture homogeneity,
Enzyme granulate fater disintegration, longer holding time, while can also reduce granulation production manufacturing cost.
Invention content
The purpose of the present invention is to provide a kind of more energy-saving and environment-friendly coating type enzyme granulates and preparation method thereof.
The first aspect of the present invention, provides a kind of coating type enzyme granulate, and the coating type enzyme granulate includes enzyme ball and packet
Clothing, the enzyme ball contain enzyme component and stabilizer, the enzyme component be selected from the group in it is one or more：
Alkali protease, alkaline lipase, cellulase, amylase and mannase；
The stabilizer be selected from the group in it is one or more：
Glucose, carboxymethyl cellulose, polyethylene glycol, sodium dihydrogen phosphate, magnesium chloride, sodium chloride and starch.
In another preferred example, the component of the coating is selected from the group below one or more：
Sodium dihydrogen phosphate, polyvinyl alcohol, PVP K30, titanium dioxide, magnesium chloride, starch and bright orchid.
In another preferred example, enzyme ball and the ratio of weight and number of coating material are 40-90 in the coating type enzyme granulate：5-
20。
In another preferred example, the ratio of weight and number of the enzyme component and the stabilizer is 30-55：35-70；It is preferred that
The ratio of weight and number of ground, the enzyme component and the stabilizer is 45：55.
In another preferred example, it is one or more during the stabilizer is selected from the group：
Glucose 2-5 parts by weight, carboxymethyl cellulose 2.5-12 parts by weight, polyethylene glycol PEG-1500 3-7 parts by weight,
Sodium dihydrogen phosphate 6-15 parts by weight, magnesium chloride 10-20 parts by weight, sodium chloride 0-5 parts by weight and cornstarch 15-28 parts by weight.
In another preferred example, it is one or more during the stabilizer is selected from the group：
3.5 parts by weight of glucose, 3.2 parts by weight of carboxymethyl cellulose, 5 parts by weight of polyethylene glycol PEG-1500, di(2-ethylhexyl)phosphate
18 parts by weight of 11 parts by weight of hydrogen sodium, 14 parts by weight of magnesium chloride and cornstarch.
In another preferred example, the component of the coating is selected from the group below one or more：
Sodium dihydrogen phosphate 6-15 parts by weight, polyvinyl alcohol PEG-1000 4-8 parts by weight, PVP K30 15-21 parts by weight,
Magnesium chloride 12-20 parts by weight, starch 10-25 parts by weight, titanium dioxide 4-9 parts by weight and bright orchid 0.05-0.1 parts by weight；Preferably,
The following group it is one or more：13 parts by weight of sodium dihydrogen phosphate, 5 parts by weight of polyvinyl alcohol PEG-1000,18 weight of PVP K30
Part, 18 parts by weight of magnesium chloride, 24. parts by weight of starch, 6 parts by weight of titanium dioxide, bright blue 0.06 parts by weight.
The second aspect of the present invention provides a kind of side of coating type enzyme granulate prepared as described in the first aspect of the invention
Method includes the following steps：
(1) enzyme ball is prepared
After enzyme component and stabilizer are mixed in proportion, wet enzyme ball is prepared using wet-method extrusion prilling process；
(2) enzyme ball dry coationg
Coating components are prepared in proportion, are dissolved in the water, and the suspension of 4-10% is made；The wet enzyme ball of preparation is placed in stream
Change in bed, drying is coated when reaching 45 DEG C -50 DEG C to temperature of charge.
In another preferred example, in the step (1), including step：
(1.1) wet feed is prepared
Enzyme with stabilizer is weighed in proportion and mixes to obtain enzyme ball material, adds in water, the weight ratio of enzyme ball material and water is 3-
5：1, it is uniformly mixed to obtain wet feed；
(1.2) round as a ball pelletization is squeezed
Wet feed made of (1.1) is put in extruder, through being squeezed into cylindrical strip extrudate；Extrudate is placed in round as a ball
It is round as a ball into wet enzyme ball on machine.
In another preferred example, in the step (1.2), extruder extrudate temperature≤80 DEG C are controlled；Preferably ,≤
70℃；It is highly preferred that≤60 DEG C；DEG C most preferably ,≤50.
In another preferred example, a diameter of 0.3-0.6mm of the extrudate is controlled in the step (1.2).
In another preferred example, in step (2) coating process, 45 DEG C -75 DEG C of control fluid bed inlet air temperature, air quantity
60m3/h-170m3/ h, atomizing pressure 1.25bar-2.5bar.
It should be understood that within the scope of the present invention, above-mentioned each technical characteristic of the invention and have in below (eg embodiment)
It can be combined with each other between each technical characteristic of body description, so as to form new or preferred technical solution.As space is limited, exist
This no longer tires out one by one states.
Specific embodiment
The present inventor and in-depth study, obtains a kind of coating type enzyme granulate prescription, which is particularly suitable for by extensive
Coating type enzyme granulate is prepared in using wet method extruder grain technique.The present invention is completed on this basis.
The object of the present invention is to provide a kind of washing enzyme granulation preparations, and dust harzard is granulated so as to solve tradition,
It is proposed a kind of safe and simple, efficient preparation method.The coating type washing that a kind of homogeneity is excellent, product quality is stablized is provided
Use enzyme preparation.One aspect of the present invention is related to cleaning solution enzyme wet granulation, includes mass percent for 40%-90% enzymes
Grain, mass percent are the coating material of 5%-20%.
Wherein enzyme granulate can be alkali protease, alkaline lipase, cellulase, amylase, mannase decile
Solve enzyme.Glucose, carboxymethyl cellulose, polyethylene glycol, sodium dihydrogen phosphate, magnesium chloride, sodium chloride, starch composition, coating components
By sodium dihydrogen phosphate, polyvinyl alcohol, PVP K30, titanium dioxide, magnesium chloride, starch, bright orchid.
The optimum quality of above-mentioned enzyme ball component is:Washing enzyme alkali protease, alkaline lipase, cellulase, starch
The 30%-55% such as enzyme, mannase, glucose 2%-5%, carboxymethyl cellulose 2.5%-12%, polyethylene glycol PEG-
1500 3%-7%, sodium dihydrogen phosphate 6%-15%, magnesium chloride 10%-20%, cornstarch 15%-28%.Wherein enzyme ball is most
High quality component is：Washing enzyme alkali protease, alkaline lipase, cellulase, amylase, mannase etc. 45%,
Glucose 3.5%, carboxymethyl cellulose 3.2%, polyethylene glycol PEG-1500 5%, sodium dihydrogen phosphate 11%, magnesium chloride 14%,
Cornstarch 18.3%.
Above-mentioned coating components sodium dihydrogen phosphate 6%-15%, polyvinyl alcohol PEG-1000 4%-8%, povidone
K3015%-21%, magnesium chloride 12%-20%, starch 10%-25%, titanium dioxide 4%-9%, bright orchid 0.05%-0.1%, on
Coating components optimal proportion is stated as sodium dihydrogen phosphate 13%, polyvinyl alcohol PEG-1000 5%, PVP K30 18%, magnesium chloride
18%, starch 24.04%, titanium dioxide 6%, bright blue 0.06%
1st, the making step of enzyme ball is as follows：
1.1st, the preparation of wet feed enzyme and auxiliary material such as microcrystalline cellulose, glucose are smashed, are sieved, after weigh in proportion
Addition is uniformly mixed, and adds in aqueous solution as coagulating agent or adhesive, the mass ratio control of material and water is 3.5：1, by powder
Material is made the uniform material of moistening with certain plasticity or wet feed is made wet granular through comminutor.Wet method requires to have
Certain moisture-retaining capacity is so as to increase the plasticity of the mobility of extrusion and round as a ball property.
1.2nd, extrusion process puts plasticity wet feed made of the first step or wet grain in extruder, through screw propulsion or rolles over rolling etc.
Wet feed by having the hole of certain diameter or sieve, wherein hole sizer diameter selection 0.45mm, is squeezed into cylindrical strip by fashion of extrusion
Extrudate.Squeezing out bar answers surface smooth, and extrudate often answers extrusion pressure to generate heat, and long-term continuous production, fever phenomenon is more
Significantly.The method of the present invention selects the extruder of sub-cooled, and the continuous big production for thermal sensitivity enzyme provides reliable technology and protects
Card.
1.3rd, above-mentioned extrudate heap is discharged on the rotation friction plate of spheronizator by round as a ball pelletization process, and extrudate is then disperseed
The smaller cylinder of its diameter is equivalent into length, due to the effect of frictional force, these plastic cylindrical materials are onboard not
It rolls with stopping, is gradually rolled into spheroidal.Its round as a ball process, round as a ball process can be distinguished several different according to shape of particle
Stage.Round as a ball pelletization process is the short cylindrical shape particle by originating, and gradually becomes no corner angle under rotary rolling on friction plate
Edge is circular cylinder, and then is rolled into dumb-bell shape, ellipse, until as fully spherical shape.Edge is being formed when round as a ball
After the cylinder of rounding, cylinder bends, reverses, subsequent cylinder ruptures the part to form two separation, both has
One round and flat face, due to the effect of rotary force and frictional force, above-mentioned planar recess, such as same flower-shape into cave, Ran Houbian
Edge packet, which stacks, is rolled into spherical shape.
2nd, enzyme ball dry coationg：The wet enzyme ball of preparation is placed in fluid bed, 45 DEG C -75 DEG C of inlet air temperature, air quantity are set
100m3/h-160m3/ h, drying are coated when reaching 45 DEG C -50 DEG C to temperature of charge, and wherein the preparation of coating solution is with reference to once
Principle：It weighs and is dissolved in water after coating powder sieving, be configured to the sieving of 4%-10% suspension, then condition fluid bed is into wind-warm syndrome
45 DEG C -75 DEG C of degree, air quantity 60m3/h-170m3/ h, atomizing pressure 1.25bar-2.5bar.
Main advantages of the present invention are：
(1) coating type enzyme granulate is prepared using wet-method extrusion prilling for the first time；
(2) a kind of coating type enzyme granulate prescription for being suitable for wet-method extrusion prilling is provided；
(3) prescription using the present invention and the wet-method extrusion prilling process of the present invention is coordinated to prepare coating type enzyme granulate, it is real
Show that detersive enzyme is safe and simple, efficient preparation, realized self raising flour dirt or be granulated without dust, improve the steady of production technology
It is qualitative, the problems such as reducing influence of the human factor to product quality and yield.
(4) technical solution provided by the invention, when enzyme granulate has preferable mixture homogeneity, enzyme granulate disintegration with washing powder
Between it is short, the holding time is long, the coating type enzyme granulate homogeneity of preparation is good, stable quality.
(5) technical scheme of the present invention more energy conservation and environmental protection, entire technological operation is easy, it is extremely low that technical experience is required.
Detection method
1st, enzyme activity：The detection of enzyme activity is carried out according to industrial enzyme preparation GENERAL EXPERIMENTATION (QB/T 1803-1993).
Enzyme activity storage rate：
X=E1/ E × 100%
X:The enzyme activity storage rate % of sample
E1：Sample actual measurement enzyme activity (u/g or u/ml)
E：Sample marker (before preservation) enzyme activity (u/g or u/ml)
2nd, dissolubility：By enzyme granulate and water with 1: 100 ratio into the water, magnetic agitation mixes slowly at room temperature, record
Enzyme granulate disintegration time.
3rd, loss on drying：Weightless inspection is dried according to industrial enzyme preparation universal test method (QB/T 1803-1993)
It surveys.
4th, stability：It is preserved 9 months under 37 DEG C, 70% damp condition, detects enzyme activity storage rate.
5th, homogeneity：Grab sample 1kg, is sieved with standard screen, the detection mesh particle accounting of 30 mesh~60.
With reference to specific embodiment, the present invention is further explained.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.Unless otherwise stated, otherwise percentage and number are calculated by weight.
Embodiment 1
(1) material prepares
Prepare material according to parts by weight listed below.
The parts by weight of each component are in enzyme ball:
Enzyme component：45 parts by weight of alkali protease；
Stabilizer component：3.5 parts by weight of glucose, 3.2 parts by weight of carboxymethyl cellulose, 5 weights of polyethylene glycol PEG-1500
Measure part, 11 parts by weight of sodium dihydrogen phosphate, 14 parts by weight of magnesium chloride, 18.3 parts by weight of cornstarch.
The parts by weight of each component are in coating：
13 parts by weight of sodium dihydrogen phosphate, 5 parts by weight of polyvinyl alcohol PEG-1000,18 parts by weight of PVP K30, magnesium chloride
18 parts by weight, 24.04 parts by weight of starch, 6 parts by weight of titanium dioxide, bright blue 0.06 parts by weight.
(2) coating type enzyme granulate is prepared
1st, the making step of enzyme ball is as follows：
1.1st, the preparation of wet feed
Enzyme is smashed respectively with stabilizer, be sieved after weigh in proportion, be uniformly mixed, add in aqueous solution as coagulating agent or
The mass ratio of adhesive, material and water is 3.5：1, wet feed is obtained after mixing.Using above-mentioned enzyme ball formula, in the material water
Stronger than lower wet feed moisture-retaining capacity obtained, mobility is moderate, and plasticity is good.
1.2nd, extrusion process
Plasticity wet feed made of the first step or wet grain are put in extruder, it will be wet through the fashion of extrusion such as screw propulsion or rolling rolling
Material is squeezed into cylindrical strip extrudate by having the hole of certain diameter or sieve, wherein hole sizer diameter selection 0.45mm.Squeeze out item
Shape object surface is smooth, and control extrudate temperature is 55 DEG C.
1.3rd, round as a ball pelletization process
Above-mentioned extrudate heap is discharged on the rotation friction plate of spheronizator, extrudate is then dispersed into length and is equivalent to it directly
The smaller cylinder of diameter, due to the effect of frictional force, these plastic cylindrical materials are onboard ceaselessly rolled, are gradually rolled into
Spheroidal.
2nd, enzyme ball dry coationg
The wet enzyme ball of preparation is placed in fluid bed, 45 DEG C -75 DEG C of inlet air temperature, air quantity 100m are set3/h-160m3/ h,
Drying is coated when reaching 45 DEG C -50 DEG C to temperature of charge, and the preparation method of wherein coating solution is：It is molten after coating components sieving
Solution in water, is configured to the sieving of 4%-10% suspension, then 45 DEG C -75 DEG C of fluid bed inlet air temperature, air quantity 60m3/h-
170m3/ h, atomizing pressure 1.25bar-2.5bar.Coating type enzyme granulate is obtained after enzyme ball is coated.
After testing, enzyme activity loss about 6.1% in preparation process.
Embodiment 2
(1) material prepares
Prepare material according to parts by weight listed below.
The parts by weight of each component are in enzyme ball:
Enzyme component：55 parts by weight of alkaline lipase；
Stabilizer component：4.5 parts by weight of glucose, 4.0 parts by weight of carboxymethyl cellulose, polyethylene glycol PEG-15004.5
Parts by weight, 8 parts by weight of sodium dihydrogen phosphate, 20 parts by weight of magnesium chloride, 25 parts by weight of cornstarch.
The parts by weight of each component are in coating：
15 parts by weight of sodium dihydrogen phosphate, 8 parts by weight of polyvinyl alcohol PEG-1000,20 parts by weight of PVP K30, magnesium chloride
12 parts by weight, 14 parts by weight of starch, 5 parts by weight of titanium dioxide, bright blue 0.05 parts by weight.
(2) coating type enzyme granulate is prepared
1st, the making step of enzyme ball is as follows：
1.1st, the preparation of wet feed
Enzyme is smashed respectively with stabilizer, be sieved after weigh in proportion, be uniformly mixed, add in aqueous solution as coagulating agent or
The mass ratio of adhesive, material and water is 4.5：1, wet feed is obtained after mixing.Using above-mentioned enzyme ball formula, in the material water
Stronger than lower wet feed moisture-retaining capacity obtained, mobility is moderate, and plasticity is good.
1.2nd, extrusion process
Plasticity wet feed made of the first step or wet grain are put in extruder, it will be wet through the fashion of extrusion such as screw propulsion or rolling rolling
Material is squeezed into cylindrical strip extrudate by having the hole of certain diameter or sieve, wherein hole sizer diameter selection 0.45mm.Squeeze out item
Shape object surface is smooth, and control extrudate temperature is 65 DEG C.
1.3rd, round as a ball pelletization process
Above-mentioned extrudate heap is discharged on the rotation friction plate of spheronizator, extrudate is then dispersed into length and is equivalent to it directly
The smaller cylinder of diameter, due to the effect of frictional force, these plastic cylindrical materials are onboard ceaselessly rolled, are gradually rolled into
Spheroidal.
2nd, enzyme ball dry coationg
The wet enzyme ball of preparation is placed in fluid bed, 45 DEG C -75 DEG C of inlet air temperature, air quantity 100m are set3/h-160m3/ h,
Drying is coated when reaching 45 DEG C -50 DEG C to temperature of charge, and the preparation method of wherein coating solution is：It is molten after coating components sieving
Solution in water, is configured to the sieving of 4%-10% suspension, then 45 DEG C -75 DEG C of fluid bed inlet air temperature, air quantity 60m3/h-
170m3/ h, atomizing pressure 1.25bar-2.5bar.Coating type enzyme granulate is obtained after enzyme ball is coated.
After testing, enzyme activity loss about 8.4% in preparation process.
Embodiment 3
(1) material prepares
Prepare material according to parts by weight listed below.
The parts by weight of each component are in enzyme ball:
Enzyme component：35 parts by weight of amylase；
Stabilizer component：2.5 parts by weight of glucose, 3.0 parts by weight of carboxymethyl cellulose, polyethylene glycol PEG-1500 6.5
Parts by weight, 14 parts by weight of sodium dihydrogen phosphate, 15 parts by weight of magnesium chloride, 20 parts by weight of cornstarch.
The parts by weight of each component are in coating：
10 parts by weight of sodium dihydrogen phosphate, 5 parts by weight of polyvinyl alcohol PEG-1000,15 parts by weight of PVP K30, magnesium chloride
15 parts by weight, 25 parts by weight of starch, 6 parts by weight of titanium dioxide, bright blue 0.1 parts by weight.
(2) coating type enzyme granulate is prepared
1st, the making step of enzyme ball is as follows：
1.1st, the preparation of wet feed
Enzyme is smashed respectively with stabilizer, be sieved after weigh in proportion, be uniformly mixed, add in aqueous solution as coagulating agent or
The mass ratio of adhesive, material and water is 5：1, wet feed is obtained after mixing.Using above-mentioned enzyme ball formula, in the material-water ratio
Lower wet feed moisture-retaining capacity obtained is strong, and mobility is moderate, and plasticity is good.
1.2nd, extrusion process
Plasticity wet feed made of the first step or wet grain are put in extruder, it will be wet through the fashion of extrusion such as screw propulsion or rolling rolling
Material is squeezed into cylindrical strip extrudate by having the hole of certain diameter or sieve, wherein hole sizer diameter selection 0.45mm.Squeeze out item
Shape object surface is smooth, and control extrudate temperature is 60 DEG C.
1.3rd, round as a ball pelletization process
Above-mentioned extrudate heap is discharged on the rotation friction plate of spheronizator, extrudate is then dispersed into length and is equivalent to it directly
The smaller cylinder of diameter, due to the effect of frictional force, these plastic cylindrical materials are onboard ceaselessly rolled, are gradually rolled into
Spheroidal.
2nd, enzyme ball dry coationg
The wet enzyme ball of preparation is placed in fluid bed, 45 DEG C -75 DEG C of inlet air temperature, air quantity 100m are set3/h-160m3/ h,
Drying is coated when reaching 45 DEG C -50 DEG C to temperature of charge, and the preparation method of wherein coating solution is：It is molten after coating components sieving
Solution in water, is configured to the sieving of 4%-10% suspension, then 45 DEG C -75 DEG C of fluid bed inlet air temperature, air quantity 60m3/h-
170m3/ h, atomizing pressure 1.25bar-2.5bar.Coating type enzyme granulate is obtained after enzyme ball is coated.
After testing, enzyme activity loss about 10.1% in preparation process.
Embodiment 4
Enzyme component is replaced with：10 parts by weight of alkali protease, 10 parts by weight of cellulase, 10 parts by weight of amylase and sweet
Reveal 10 parts by weight of dextranase；Other components and technique are same as Example 1.
After the completion of preparation, detection preparation process neutral and alkali prolease activity loss about 3.4%, amylase activity loses about
It is 5.3%.
Embodiment 5
Enzyme component is replaced with：10 parts by weight of alkaline lipase, 10 parts by weight of cellulase, 10 parts by weight of amylase and sweet
Reveal 10 parts by weight of dextranase；Other components and technique are same as Example 2.
After the completion of preparation, detection preparation process neutral and alkali lipase activity loss about 4.5%, amylase activity loses about
It is 4.6%.
Comparative example 1
Prepare material according to parts by weight listed below.
The parts by weight of each component are in enzyme ball:
Enzyme component：45 parts by weight of alkali protease；
Stabilizer component：15 parts by weight of dextrin, 25 parts by weight of bean powder, 1 parts by weight of lactose, 3 weight of dodecyl sodium sulfate
Part, 6 parts by weight of carboxymethyl cellulose, 20 parts by weight of cornstarch.
The parts by weight of each component are in coating：
30 parts by weight of dextrin, 10 parts by weight of starch, 15 parts by weight of polyvinyl alcohol, 1 parts by weight of dodecyl sodium sulfate, sulfuric acid
20 parts by weight of sodium, 20 parts by weight of titanium dioxide, bright blue 0.01 parts by weight.
Preparation process and method are with embodiment 1, and after the completion of preparation, the loss of detection preparation process neutral and alkali prolease activity is about
It is 18.6%.
Quality testing is carried out to the product prepared in embodiment 1-5 and comparative example 1, has detected homogeneity, dissolving respectively
Property, loss on drying and stability, testing result are as shown in table 1.
1 coating type enzyme granulate quality testing of table
| Group | Homogeneity | Disintegration time | Loss on drying | Stability |
| Embodiment 1 | 99% | 40s | 4% | 92% |
| Embodiment 2 | 98% | 44s | 5% | 94% |
| Embodiment 3 | 98% | 45s | 5% | 91% |
| Embodiment 4 | 98% | 48s | 6% | 95%* |
| Embodiment 5 | 98% | 40s | 6% | 96%** |
| Comparative example 1 | 98% | 138s | 6% | 82% |
Note：* in terms of protease；* is in terms of lipase.
Prescription using the present invention simultaneously coordinates the wet-method extrusion prilling process of the present invention to prepare coating type enzyme granulate, realizes
Detersive enzyme is safe and simple, efficient preparation, realizes self raising flour dirt or is granulated without dust, improves the stability of production technology,
Influence of the human factor to product quality and yield is reduced, enzyme activity is lost in 4-8% or so in preparation process, in 37 DEG C of guarantors
The enzyme activity deposited 9 months is able to maintain that more than 90%.By enzyme granulate and water with 1: 100 ratio into the water, magnetic force stirs at room temperature
It mixes and mixes slowly, record enzyme granulate disintegration time.The disintegration time of this product granulated pellet is same better than both at home and abroad within 50s
Class product, grab sample 1kg, is sieved with standard screen, and detection 30 mesh~60 mesh particle accounting example reaches 98%.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To be made various changes or modifications to the present invention, such equivalent forms equally fall within the model that the application the appended claims are limited
It encloses.
Claims (12)
1. a kind of coating type enzyme granulate, which is characterized in that the coating type enzyme granulate includes enzyme ball and coating, and the enzyme ball contains
Enzyme component and stabilizer, the enzyme component be selected from the group in it is one or more：
Alkali protease, alkaline lipase, cellulase, amylase and mannase；
The stabilizer is by glucose 2-5 parts by weight, carboxymethyl cellulose 2.5-12 parts by weight, polyethylene glycol PEG-1500 3-7
Parts by weight, sodium dihydrogen phosphate 6-15 parts by weight, magnesium chloride 10-20 parts by weight, sodium chloride 0-5 parts by weight and cornstarch 15-28
Parts by weight form；
The component of the coating is by sodium dihydrogen phosphate, polyvinyl alcohol, PVP K30, titanium dioxide, magnesium chloride, starch and bright blue group
Into；
Also, enzyme ball and the ratio of weight and number of coating material are 40-90 in the coating type enzyme granulate：5-20.
2. coating type enzyme granulate as described in claim 1, which is characterized in that the enzyme component and the parts by weight of the stabilizer
Number is than being 30-55：35-70.
3. coating type enzyme granulate as claimed in claim 2, which is characterized in that the enzyme component and the parts by weight of the stabilizer
Number is than being 45：55.
4. coating type enzyme granulate as described in claim 1, which is characterized in that the stabilizer is by 3.5 parts by weight of glucose, carboxylic
3.2 parts by weight of methylcellulose, 5 parts by weight of polyethylene glycol PEG-1500,11 parts by weight of sodium dihydrogen phosphate, 14 weight of magnesium chloride
Part and 18 parts by weight of cornstarch composition.
5. coating type enzyme granulate as described in claim 1, which is characterized in that the component of the coating is by sodium dihydrogen phosphate 6-15
Parts by weight, polyvinyl alcohol PEG-1000 4-8 parts by weight, PVP K30 15-21 parts by weight, magnesium chloride 12-20 parts by weight are formed sediment
Powder 10-25 parts by weight, titanium dioxide 4-9 parts by weight and bright orchid 0.05-0.1 parts by weight composition.
6. coating type enzyme granulate as described in claim 1, which is characterized in that the component of the coating is weighed by sodium dihydrogen phosphate 13
Measure part, 5 parts by weight of polyvinyl alcohol PEG-1000,18 parts by weight of PVP K30,18 parts by weight of magnesium chloride, 24.04 weight of starch
Part, 6 parts by weight of titanium dioxide and bright blue 0.06 parts by weight composition.
A kind of 7. method for preparing the coating type enzyme granulate as described in claim 1-6 is any, which is characterized in that including walking as follows
Suddenly：
(1) enzyme ball is prepared
After enzyme component and stabilizer are mixed in proportion, wet enzyme ball is prepared using wet-method extrusion prilling process；
(2) enzyme ball dry coationg
Coating components are prepared in proportion, are dissolved in the water, and the suspension of 4-10% is made；The wet enzyme ball of preparation is placed in fluid bed
In, drying is coated when reaching 45 DEG C -50 DEG C to temperature of charge,
Wherein, in the step (1), including step：
(1.1) wet feed is prepared
Enzyme with stabilizer is weighed in proportion and mixes to obtain enzyme ball material, adds in water, the weight ratio of enzyme ball material and water is 3-5：1,
It is uniformly mixed to obtain wet feed；
(1.2) round as a ball pelletization is squeezed
Wet feed made of (1.1) is put in extruder, through being squeezed into cylindrical strip extrudate；Extrudate is placed in spheronizator
On, it is round as a ball into wet enzyme ball.
8. the method for claim 7, which is characterized in that in the step (1.2), control extruder extrudate temperature
≤80℃。
9. the method for claim 7, which is characterized in that the diameter of the control extrudate in the step (1.2)
For 0.3-0.6mm.
10. method as claimed in claim 8, which is characterized in that in the step (1.2), control extruder extrudate temperature
≤ 70 DEG C of degree.
11. method as claimed in claim 8, which is characterized in that in the step (1.2), control extruder extrudate temperature
≤ 60 DEG C of degree.
12. method as claimed in claim 8, which is characterized in that in the step (1.2), control extruder extrudate temperature
≤ 50 DEG C of degree.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440361A (en) * | 2007-11-22 | 2009-05-27 | 珠海百康生物技术有限公司 | Method for preparing stable coated granzyme |
| CN102511665A (en) * | 2011-12-29 | 2012-06-27 | 青岛蔚蓝生物集团有限公司 | Phytase-coated pellets and preparation method thereof |
| CN103555706A (en) * | 2013-10-17 | 2014-02-05 | 青岛蔚蓝生物集团有限公司 | Production method of coated enzyme granules |
| CN103571172A (en) * | 2013-10-17 | 2014-02-12 | 青岛蔚蓝生物集团有限公司 | Coating composition of enzyme grains |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440361A (en) * | 2007-11-22 | 2009-05-27 | 珠海百康生物技术有限公司 | Method for preparing stable coated granzyme |
| CN102511665A (en) * | 2011-12-29 | 2012-06-27 | 青岛蔚蓝生物集团有限公司 | Phytase-coated pellets and preparation method thereof |
| CN103555706A (en) * | 2013-10-17 | 2014-02-05 | 青岛蔚蓝生物集团有限公司 | Production method of coated enzyme granules |
| CN103571172A (en) * | 2013-10-17 | 2014-02-12 | 青岛蔚蓝生物集团有限公司 | Coating composition of enzyme grains |
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