CN104277905A - Sea dog liver oil - Google Patents
Sea dog liver oil Download PDFInfo
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- CN104277905A CN104277905A CN201410538898.8A CN201410538898A CN104277905A CN 104277905 A CN104277905 A CN 104277905A CN 201410538898 A CN201410538898 A CN 201410538898A CN 104277905 A CN104277905 A CN 104277905A
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- sea dog
- liver oil
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- dog liver
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Fats And Perfumes (AREA)
- Edible Oils And Fats (AREA)
Abstract
The invention relates to the technical field of medical food processing and particularly relates to a method of producing liver oil by using livers of sea dogs. The sea dog liver oil comprises 30-40% (mass) of omega-3 unsaturated fatty acid, 0.75-0.9% (mass) of squalene, 1000-1500UI/g of VE, 1200-1600IU/g of VA, 160-250IU/g of VD and the balance of lipid. A preparation method of the sea dog liver oil comprises the following steps: taking the livers of the sea dog as raw materials, adopting a pretreatment method of freezing and unfreezing gain and a method of freezing, unfreezing and emulsion breaking, using compound protease to extract crude cod-liver oil; then, adopting a refining process of degumming by citric acid and deacidifying by a mixture of caustic soda and soda ash. The production method of the sea dog liver oil is reasonable in operation, low in cost and mild in extracting condition; an extraction rate of the crude sea dog liver oil can be over 85% and the recovery rate of the refined cod-liver oil can be over 65%.
Description
Technical field
The present invention relates to dietetic food processing technique field, a kind of sea dog liver that utilizes is to produce the method for liver oil specifically.
Background technology
Sea dog is the general designation to Pinnipedia kind Phocidae animal, this is the Mammals that a class health becomes spindle build, four limbs specialization becomes fin-shaped, the head circle neck of sea dog is short, there is no outer auricle, because their face head as cat thus sea dog of gaining the name, to distinguish the animal of other Liang Ge sections of Pinnipedia (Otariidae, Odobenidae).They highly adapt to the life in ocean, and most time is movable in ocean, spreads all over whole marine site, maximum with the littoral quantity in the South Pole.Common sea dog has: harbor seal, harp seal, hat sea dog etc.The economic worth of sea dog is high, but it is in marine food chain top, is all enriched a large amount of mercury in body, has eaten and can cause chronic poisoning.Cortex is tough and tensile, can be used for making clothes, footwear, cap etc. and resist severe cold.
Seal oil is a kind of precious nutritional supplements being rich in omega-3 unsaturated fatty acid from seal fat tissue extraction.Containing ω-3 unsaturated fatty acids of about 20% ~ 25% in seal oil, its content be occurring in nature animal.Meanwhile, in seal oil, a certain amount of squalene and vitamin-E is contained.
Summary of the invention
Primary technical problem to be solved by this invention is to provide a kind of omega-3 unsaturated fatty acid and squalene, V
e, also have V
a, V
dsea dog liver oil.
For achieving the above object, the technical solution used in the present invention is: a kind of sea dog liver oil, and described liver oil comprises omega-3 unsaturated fatty acid 30 ~ 40%(quality), squalene 0.75 ~ 0.9%(quality), V
econtent is 1000-1500UI/g, V
acontent is 1200 ~ 1600IU/g, V
dcontent is 160 ~ 250IU/g, and other material is lipid.
Further, described liver oil comprises omega-3 unsaturated fatty acid 33 ~ 38%(quality), squalene 0.8 ~ 0.9%(quality), V
econtent is 1200-1500UI/g, V
acontent is 1400 ~ 1600IU/g, V
dcontent is 180 ~ 230IU/g, and other material is lipid.
Further, described liver oil comprises omega-3 unsaturated fatty acid 36%(quality), squalene 0.85%(quality), V
econtent is 1300UI/g, V
acontent is 1450IU/g, V
dcontent is 210IU/g, and other material is lipid.
Further, in described omega-3 unsaturated fatty acid, EPA and DHA content are 30 ~ 35%(weight).
A preparation method for sea dog liver oil, comprises the steps:
1) storage of sea dog liver: by sea dog processing byproduct sea dog liver, after cleaning, less than-18 ~-22 DEG C chilled storages;
2) pre-treatment: sea dog liver is thawed, chopping;
3) enzymolysis: add water in hydrolyzer, liquid-solid ratio (mL/g) is made to be 1:1 ~ 2:1, salt adding acid for adjusting pH is 5.5 ~ 6.5, add sea dog liver 1.5 ~ 2.5%(quality) compound protease, sealing, being placed in constant temperature oscillator, is 100 ~ 150r/min at rotating speed, enzymolysis 3 ~ 4h at temperature 40 ~ 50 DEG C;
4) to go out centrifugation after ferment treatment: enzymolysis terminates, salt adding acid for adjusting pH to 3 ~ 3.5, make compound protease deactivation, are placed in low speed large capacity centrifuge, at rotating speed 4000 ~ 5000r/min centrifugal 5 ~ 15min while hot, be separated to obtain the thick sea dog liver oil in upper strata; The middle level lipoprotein milk sap of first time centrifugal generation adopts freeze and unfreeze breaking method, and namely enzymolysis solution freezes 10 ~ 14h at-18 ~-22 DEG C, and 38 ~ 42 DEG C of 1 ~ 2h that thaw carry out breakdown of emulsion, and then centrifugation;
5) refining: come unstuck with the citric acid of 700 ~ 900g/L, addition is 0.5 ~ 1.5%(volume of oil mass); With caustic soda-soda ash mixture (mass ratio 1.8 ~ 2.2:1) depickling of 250 ~ 350g/L, addition is oil mass 1.5 ~ 2.5%(volume); With activated decoloration, addition is 8 ~ 11%(quality of oil mass); At 80 ~ 90 DEG C, underpressure distillation 5 ~ 15min carries out deodorization;
6) subzero treatment: refining sea dog liver oil is slowly dropped to 3 ~ 8 DEG C within the time of 3 ~ 5d, then drops to-2 DEG C ~-3 DEG C, needs 10 ~ 20h, and the solid fat of elimination obtains sea dog liver edible vegetable oil, then adopts to vacuumize and rushes nitrogen packaging.
Advantageous Effects of the present invention is: the present invention with sea dog liver for raw material, adopt again the pre-treating process of freeze and unfreeze and the method for freeze and unfreeze breakdown of emulsion, use the thick Oils,glyceridic,cod-liver of conjugated protein enzyme extraction, then citric acid is adopted to come unstuck and the refinery practice of caustic soda-soda ash mixture depickling, rationally, cost is low in operation, and extraction conditions is gentle, the extraction yield of thick sea dog liver oil can reach more than 85%, and the rate of recovery of prepared fiss liver oil can reach more than 65%.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
A kind of sea dog liver oil, comprises omega-3 unsaturated fatty acid 40%(quality), squalene 0.75%(quality), V
econtent is 1500UI/g, V
acontent is 1200IU/g, V
dcontent is 250IU/g, and other material is lipid.
Wherein, in omega-3 unsaturated fatty acid, EPA and DHA content are 30%(weight).
A preparation method for sea dog liver oil, comprises the steps:
1) storage of sea dog liver: by sea dog processing byproduct sea dog liver, after cleaning, less than-22 DEG C chilled storages;
2) pre-treatment: sea dog liver is thawed, chopping;
3) enzymolysis: add water in hydrolyzer, make liquid-solid ratio (mL/g) be 1:1, salt adding acid for adjusting pH is 5.5, add sea dog liver 2.5%(quality) papoid, sealing, be placed in constant temperature oscillator, be 100r/min at rotating speed, enzymolysis 4h under temperature 50 C;
4) to go out centrifugation after ferment treatment: enzymolysis terminates, salt adding acid for adjusting pH to 3.5, makes compound protease deactivation, is placed in low speed large capacity centrifuge, at rotating speed 40000r/min centrifugal 15min while hot, is separated to obtain the thick sea dog liver oil in upper strata; The middle level lipoprotein milk sap of first time centrifugal generation adopts freeze and unfreeze breaking method, and namely enzymolysis solution freezes 10h at-18 DEG C, and 42 DEG C of 1h that thaw carry out breakdown of emulsion, and then centrifugation;
5) refining: come unstuck with the citric acid of 700g/L, addition is the 1.5%(volume of oil mass); With caustic soda-soda ash mixture (mass ratio 1.8:1) depickling of 350g/L, addition is oil mass 1.5%(volume); With activated decoloration, addition is the 8%(quality of oil mass); At 90 DEG C, underpressure distillation 5min carries out deodorization;
6) subzero treatment: refining sea dog liver oil is slowly dropped to 8 DEG C within the time of 3d, then drops to-2 DEG C DEG C, need 10h, the solid fat of elimination obtains sea dog liver edible vegetable oil, then adopts to vacuumize to rush nitrogen packaging.
Embodiment 2
A kind of sea dog liver oil, comprises omega-3 unsaturated fatty acid 40%(quality), squalene 0.9%(quality), V
econtent is 1500UI/g, V
acontent is 1400IU/g, V
dcontent is 230IU/g, and other material is lipid.
Wherein, in omega-3 unsaturated fatty acid, EPA and DHA content are 32%(weight).
A preparation method for sea dog liver oil, comprises the steps:
1) storage of sea dog liver: by sea dog processing byproduct sea dog liver, after cleaning, less than-20 DEG C chilled storages;
2) pre-treatment: sea dog liver is thawed, chopping;
3) enzymolysis: add water in hydrolyzer, make liquid-solid ratio (mL/g) be 2:1, salt adding acid for adjusting pH is 6, adds sea dog liver 1.5%(quality) papoid, sealing, being placed in constant temperature oscillator, is 100r/min at rotating speed, enzymolysis 4h under temperature 45 C;
4) to go out centrifugation after ferment treatment: enzymolysis terminates, salt adding acid for adjusting pH to 3.3, makes compound protease deactivation, is placed in low speed large capacity centrifuge, at rotating speed 4500r/min centrifugal 10min while hot, is separated to obtain the thick sea dog liver oil in upper strata; The middle level lipoprotein milk sap of first time centrifugal generation adopts freeze and unfreeze breaking method, and namely enzymolysis solution freezes 12h at-20 DEG C, and 40 DEG C of 1h that thaw carry out breakdown of emulsion, and then centrifugation;
5) refining: come unstuck with the citric acid of 800g/L, addition is the 1.0%(volume of oil mass); With caustic soda-soda ash mixture (mass ratio 2.0:1) depickling of 300g/L, addition is oil mass 2.0%(volume); With activated decoloration, addition is the 9%(quality of oil mass); At 85 DEG C, underpressure distillation 12min carries out deodorization;
6) subzero treatment: refining sea dog liver oil is slowly dropped to 5 DEG C within the time of 4d, then drops to-3 DEG C, need 11h, the solid fat of elimination obtains sea dog liver edible vegetable oil, then adopts to vacuumize to rush nitrogen packaging.
Embodiment 3
A kind of sea dog liver oil, comprises omega-3 unsaturated fatty acid 36%(quality), squalene 0.85%(quality), V
econtent is 1300UI/g, V
acontent is 1450IU/g, V
dcontent is 210IU/g, and other material is lipid.
Wherein, in omega-3 unsaturated fatty acid, EPA and DHA content are 35%(weight).
A preparation method for sea dog liver oil, comprises the steps:
1) storage of sea dog liver: by sea dog processing byproduct sea dog liver, after cleaning, less than-18 DEG C chilled storages;
2) pre-treatment: sea dog liver is thawed, chopping;
3) enzymolysis: add water in hydrolyzer, make liquid-solid ratio (mL/g) be 2:1, salt adding acid for adjusting pH is 6.5, add sea dog liver 2.0%(quality) papoid, sealing, be placed in constant temperature oscillator, be 125r/min at rotating speed, enzymolysis 3.5h at temperature 40 DEG C;
4) to go out centrifugation after ferment treatment: enzymolysis terminates, salt adding acid for adjusting pH to 3, makes compound protease deactivation, is placed in low speed large capacity centrifuge, at rotating speed 4000r/min centrifugal 15min while hot, is separated to obtain the thick sea dog liver oil in upper strata; The middle level lipoprotein milk sap of first time centrifugal generation adopts freeze and unfreeze breaking method, and namely enzymolysis solution freezes 14h at-22 DEG C, and 42 DEG C of 2h that thaw carry out breakdown of emulsion, and then centrifugation;
5) refining: come unstuck with the citric acid of 900g/L, addition is the 1.5%(volume of oil mass); With caustic soda-soda ash mixture (mass ratio 2.2:1) depickling of 350g/L, addition is oil mass 2.5%(volume); With activated decoloration, addition is the 11%(quality of oil mass); At 90 DEG C, underpressure distillation 15min carries out deodorization;
6) subzero treatment: refining sea dog liver oil is slowly dropped to 8 DEG C within the time of 5d, then drops to-3 DEG C, need 20h, the solid fat of elimination obtains sea dog liver edible vegetable oil, then adopts to vacuumize to rush nitrogen packaging.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. sea dog liver oil, is characterized in that, described liver oil comprises omega-3 unsaturated fatty acid 30 ~ 40%(quality), squalene 0.75 ~ 0.9%(quality), V
econtent is 1000-1500UI/g, V
acontent is 1200 ~ 1600IU/g, V
dcontent is 160 ~ 250IU/g, and other material is lipid.
2. sea dog liver oil according to claim 1, it is characterized in that, described liver oil comprises omega-3 unsaturated fatty acid 33 ~ 38%(quality), squalene 0.8 ~ 0.9%(quality), V
econtent is 1200-1500UI/g, V
acontent is 1400 ~ 1600IU/g, V
dcontent is 180 ~ 230IU/g, and other material is lipid.
3. sea dog liver oil according to claim 2, it is characterized in that, described liver oil comprises omega-3 unsaturated fatty acid 36%(quality), squalene 0.85%(quality), V
econtent is 1300UI/g, V
acontent is 1450IU/g, V
dcontent is 210IU/g, and other material is lipid.
4. sea dog liver oil according to claim 1, it is characterized in that, in described omega-3 unsaturated fatty acid, EPA and DHA content are 30 ~ 35%(weight).
5. the preparation method of sea dog liver oil according to any one of Claims 1 to 4, is characterized in that, comprise the steps:
1) storage of sea dog liver: by sea dog processing byproduct sea dog liver, after cleaning, less than-18 ~-22 DEG C chilled storages;
2) pre-treatment: sea dog liver is thawed, chopping;
3) enzymolysis: add water in hydrolyzer, liquid-solid ratio (mL/g) is made to be 1:1 ~ 2:1, salt adding acid for adjusting pH is 5.5 ~ 6.5, add sea dog liver 1.5 ~ 2.5%(quality) papoid, sealing, being placed in constant temperature oscillator, is 100 ~ 150r/min at rotating speed, enzymolysis 3 ~ 4h at temperature 40 ~ 50 DEG C;
4) to go out centrifugation after ferment treatment: enzymolysis terminates, salt adding acid for adjusting pH to 3 ~ 3.5, make compound protease deactivation, are placed in low speed large capacity centrifuge, at rotating speed 4000 ~ 5000r/min centrifugal 5 ~ 15min while hot, be separated to obtain the thick sea dog liver oil in upper strata; The middle level lipoprotein milk sap of first time centrifugal generation adopts freeze and unfreeze breaking method, and namely enzymolysis solution freezes 10 ~ 14h at-18 ~-22 DEG C, and 38 ~ 42 DEG C of 1 ~ 2h that thaw carry out breakdown of emulsion, and then centrifugation;
5) refining: come unstuck with the citric acid of 700 ~ 900g/L, addition is 0.5 ~ 1.5%(volume of oil mass); With caustic soda-soda ash mixture (mass ratio 1.8 ~ 2.2:1) depickling of 250 ~ 350g/L, addition is oil mass 1.5 ~ 2.5%(volume); With activated decoloration, addition is 8 ~ 11%(quality of oil mass); At 80 ~ 90 DEG C, underpressure distillation 5 ~ 15min carries out deodorization;
6) subzero treatment: refining sea dog liver oil is slowly dropped to 3 ~ 8 DEG C within the time of 3 ~ 5d, then drops to-2 DEG C ~-3 DEG C, needs 10 ~ 20h, and the solid fat of elimination obtains sea dog liver edible vegetable oil, then adopts to vacuumize and rushes nitrogen packaging.
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|---|---|---|---|
| CN201410538898.8A CN104277905A (en) | 2014-10-14 | 2014-10-14 | Sea dog liver oil |
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| CN201410538898.8A CN104277905A (en) | 2014-10-14 | 2014-10-14 | Sea dog liver oil |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105419932A (en) * | 2015-12-10 | 2016-03-23 | 黄丽婷 | Extraction method of fish oil |
| CN105754716A (en) * | 2016-02-25 | 2016-07-13 | 湖南科技学院 | Low-temperature extracted snake oil and method for deodorizing and de-coloring snake oil |
| CN107267276A (en) * | 2017-08-06 | 2017-10-20 | 青岛农业大学 | It is a kind of to digest the method that centrifugal process extracts Goose fat liver oil |
| CN107267277A (en) * | 2017-08-06 | 2017-10-20 | 青岛农业大学 | A kind of duck fatty liver oil and preparation method thereof |
| CN107802648A (en) * | 2017-12-14 | 2018-03-16 | 浙江海洋大学 | A kind of concocting method of the natural cod-liver oil of anti-curing osteoporosis |
| CN107981357A (en) * | 2017-12-14 | 2018-05-04 | 荣成市飞创科技有限公司 | A kind of method that sea cucumber oil capsule is prepared using sea cucumber internal organ |
| CN108148676A (en) * | 2018-02-02 | 2018-06-12 | 中国水产科学研究院黄海水产研究所 | A kind of method that cod liver oil is leached with dimethyl carbonate |
| CN109181844A (en) * | 2018-09-14 | 2019-01-11 | 许昌鑫瑞德化工科技有限公司 | A kind of modified vegetable and animals oils extracting method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103564067A (en) * | 2012-07-27 | 2014-02-12 | 浙江海洋学院 | Skate liver oil and preparation method thereof |
-
2014
- 2014-10-14 CN CN201410538898.8A patent/CN104277905A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103564067A (en) * | 2012-07-27 | 2014-02-12 | 浙江海洋学院 | Skate liver oil and preparation method thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105419932A (en) * | 2015-12-10 | 2016-03-23 | 黄丽婷 | Extraction method of fish oil |
| CN105754716A (en) * | 2016-02-25 | 2016-07-13 | 湖南科技学院 | Low-temperature extracted snake oil and method for deodorizing and de-coloring snake oil |
| CN105754716B (en) * | 2016-02-25 | 2020-02-07 | 湖南科技学院 | Method for extracting snake oil at low temperature and removing fishy smell and decoloring of snake oil |
| CN107267276A (en) * | 2017-08-06 | 2017-10-20 | 青岛农业大学 | It is a kind of to digest the method that centrifugal process extracts Goose fat liver oil |
| CN107267277A (en) * | 2017-08-06 | 2017-10-20 | 青岛农业大学 | A kind of duck fatty liver oil and preparation method thereof |
| CN107802648A (en) * | 2017-12-14 | 2018-03-16 | 浙江海洋大学 | A kind of concocting method of the natural cod-liver oil of anti-curing osteoporosis |
| CN107981357A (en) * | 2017-12-14 | 2018-05-04 | 荣成市飞创科技有限公司 | A kind of method that sea cucumber oil capsule is prepared using sea cucumber internal organ |
| CN108148676A (en) * | 2018-02-02 | 2018-06-12 | 中国水产科学研究院黄海水产研究所 | A kind of method that cod liver oil is leached with dimethyl carbonate |
| CN109181844A (en) * | 2018-09-14 | 2019-01-11 | 许昌鑫瑞德化工科技有限公司 | A kind of modified vegetable and animals oils extracting method |
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Application publication date: 20150114 |