Summary of the invention
The object of the present invention is to provide a kind of functional milks vinegar health drink being raw material with the Chinese herbal medicines such as matrimony vine and milk, it is the health-care vinegar beverage with milk taste, also be the dairy produce with acetic acid taste, the demand that people are multi-level to beverage flavor, health-care efficacy, nutrition etc. can be met.
For achieving the above object, technical scheme of the present invention is as follows:
A kind of Chinese wolfberry fruit milk vinegar health drink, it is characterized in that, the raw material selected and mass ratio are: matrimony vine 45 ~ 55 parts, 25 ~ 35 parts, milk, STEVIA REBAUDIANA 10 ~ 20 parts, wilsonii 8 ~ 12 parts, Radix Codonopsis 4 ~ 6 parts.
The invention provides a kind of production technology of Chinese wolfberry fruit milk vinegar health drink, it is characterized in that: adopt alcoholic fermentation, acetic fermentation, lactic fermentation syllogic zymotechnique.
The invention provides a kind of production technology of Chinese wolfberry fruit milk vinegar health drink, it is characterized in that: first lixiviate taked by Chinese herbal medicine, rear and syllogic ferments the two-part extraction process extracted with step microbial fermentation.
Vinegar method processed yields poorly, the production cycle is long, labour intensity is large, raw material availability is low and the technology drawback of liquid fermentation method product special flavour difference for solid-state, the invention provides a kind of Chinese wolfberry fruit milk vinegar health drink production technology, it is characterized in that alcoholic fermentation employing is solid-state, liquid state fermentation coexists zymotechnique.
A production technology for Chinese wolfberry fruit milk vinegar health drink, specifically comprises the following steps:
(1) get the raw materials ready: get the raw materials ready according to aforementioned proportion, remove infested raw material;
(2) Chinese herbal medicine lixiviate: after soaking 12h with 8 ~ 10 times of pure water, under 60 ~ 70 DEG C of conditions, with ultrasonic extractor process 20min, the active ingredient in the Chinese herbal medicines such as matrimony vine is fully leached in pure water;
(3) enzymolysis: add pectase, carbohydrase, cellulase respectively in 0.1 ~ 0.2g/100mL, 0.4 ~ 0.8g/100mL, 0.2 ~ 0.4g/100mL ratio, then at 40 ~ 60 DEG C, coordinated enzymatic hydrolysis 2 ~ 3h under stirring condition;
(4) prepare burden: add 0.15 ~ 0.20% ammonium sulfate, 0.05 ~ 0.08% potassium dihydrogen phosphate, 0.02 ~ 0.03% magnesium sulfate;
(5) alcoholic fermentation: regulate initial total sugar degree 13 ~ 15BX after enzymolysis, inoculation saccharomyces cerevisiae, inoculum concentration 6 ~ 8%, then (supernatant liquid stirs fermentation to carry out alcoholic fermentation, lower floor's solid standing for fermentation, 12h stirs once), at 20 ~ 28 DEG C of condition bottom fermentation 36 ~ 48h;
(6) acetic fermentation: inoculate acetic acid bacteria after alcoholic fermentation, inoculum concentration 8 ~ 10%, standing for fermentation 72 ~ 96h under 28 ~ 32 DEG C of conditions;
(7) be separated: be separated after fermentation ends, obtain acetic acid fermentation liquid;
(8) spray: to the solid spray be separated, acetic acid and other functional activity materials are leached as far as possible, and merging spray liquid and zymotic fluid obtain acetate solution;
(9) merge: merge milk and acetic acid fermentation liquid;
(10) lactic fermentation: inoculating lactic acid bacterium, inoculum concentration 6 ~ 10%, standing for fermentation 24 ~ 36h under 28 ~ 32 DEG C of conditions;
(11) filter: adopt diatomite filtration;
(12) sterilization: carry out high-temperature short-time sterilization after filtration, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(13) ageing: ageing 20 ~ 30 days under normal temperature condition;
(14) allocate: the total acid content 1.0 ~ 2.0% adjusting above-mentioned beverage, and add 1.0 ~ 1.5% sweeteners, 0.1% citric acid, 0.15 ~ 0.02% calcium lactate, 1 ~ 2% xanthans, 0.01 ~ 0.02% sodium Diacetate;
(15) homogeneous: deployed beverage is placed in homogenizer, at homogenization pressure 25MPa, homogeneous at temperature 60 ~ 70 DEG C, makes various nutritional labeling homogenising, prevents product from occurring layering and precipitating, product stability is further ensured;
(16) essence filter: the functional health vinegar beverage after homogeneous is first filtered through 200 order duplex strainers, then through the filter of diatom filter essence with removing impurity wherein and not tolerant;
(17) re-pasteurization: deployed functional health vinegar beverage carries out high-temperature short-time sterilization, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(18) filling: adopt vacuum filling, vacuum is 0.04-0.05Mpa, and filling temperature controls at 60-75 DEG C, then is cooled to normal temperature and gets product.
According to above-mentioned zymotechnique, before alcoholic fermentation process, do not carry out the Separation of Solid and Liquid of zymotic fluid.
In above-mentioned (14) adaptation step, sweetener is one or more in oligoisomaltose, sucrose, honey, xylitol.
The invention provides a kind of Chinese wolfberry fruit milk vinegar health drink, product color is reddish brown, sweet and sour taste, soft refrigerant smooth, mellow in taste, and juice is limpid bright, free from admixture; Not only remain the compound fragrant of matrimony vine, milk, also possessed sour and the flavor substance such as ester class that fermentable produces, have promote the production of body fluid to quench thirst, the effect such as develop immunitypty, antifatigue.Chinese wolfberry fruit milk vinegar health drink of the present invention has the oxidation resistance being significantly better than different Chinese herbal medicine formula, different process engineering, and the clearance rate of Chan Pin Dui ﹒ OH of the present invention reaches 95.88%, to O after measured
2 -the clearance rate of ﹒ reaches 70.35%, close to conventional antioxidant BHT; Results of animal shows that Chinese wolfberry health-care milk vinegar beverage of the present invention has and suppresses by H preferably
2o
2the blood haemolysis effect of induction, inhibiting rate reaches more than 85%, higher than conventional antioxidant Vc; Lipid peroxidation for LH You ﹒ OH free yl induction has very strong inhibitory action, and inhibiting rate reaches 100%.Above-mentioned action effect all has conspicuousness (p<0.01) compared with different Chinese herbal medicine formula ratio, different fermentations technique.
The invention provides a kind of Chinese wolfberry fruit milk vinegar health drink, it is advantageous that: one is that employing is solid-state, liquid state fermentation coexists zymotechnique, mix solid state fermentation raciness and liquid state fermentation output is high, with short production cycle, labour intensity is low, product health is good advantage, shorten fermentation time more than 10 days, local flavor reaches solid fermentation level; Two is that first lixiviate taked by Chinese herbal medicine, rear and syllogic ferments the two-part extraction process extracted with step microbial fermentation, extend the extraction time of Chinese herbal medicine active component, and the leaching of active component is accelerated by the fermentation of microorganism, Chinese herbal medicine functional active components leaching rate more than 98.5%, improve 27.93% than one-step fermentation technique direct after Chinese herbal medicine lixiviate, this shows that more Chinese herbal medicine functional activity material enters into health-care vinegar beverage; Three is contain STEVIA REBAUDIANA in raw material, is a kind of good natural sweetener, and has effect of lowering blood sugar, make product be more suitable for patients with diabetes mellitus; Four is mixed the advantage such as milk and vinegar beverage local flavor, effect.
Detailed description of the invention
embodiment 1:
A kind of Chinese wolfberry fruit milk vinegar health drink, its concrete production technology is as follows:
(1) get the raw materials ready: get the raw materials ready according to matrimony vine 45 parts, 25 parts, milk, STEVIA REBAUDIANA 20 parts, wilsonii 12 parts, Radix Codonopsis 6 parts of ratios, remove infested raw material;
(2) Chinese herbal medicine lixiviate: after soaking 12h with 10 times of pure water, under 60 ~ 70 DEG C of conditions, with ultrasonic extractor process 20min, the active ingredient in the Chinese herbal medicines such as matrimony vine is fully leached in pure water;
(3) enzymolysis: add pectase, carbohydrase, cellulase respectively in 0.1g/100mL, 0.4g/100mL, 0.4g/100mL ratio, then at 40 ~ 60 DEG C, coordinated enzymatic hydrolysis 2 ~ 3h under stirring condition;
(4) prepare burden: add 0.20% ammonium sulfate, 0.08% potassium dihydrogen phosphate, 0.03% magnesium sulfate;
(5) alcoholic fermentation: regulate initial total sugar degree 13 ~ 15BX after enzymolysis, inoculation saccharomyces cerevisiae, inoculum concentration 8%, then carries out alcoholic fermentation (supernatant liquid stirs fermentation, lower floor's solid standing for fermentation, and 12h stirs once), at 20 ~ 28 DEG C of condition bottom fermentation 36h;
(6) acetic fermentation: inoculate acetic acid bacteria after alcoholic fermentation, inoculum concentration 10%, standing for fermentation 72h under 28 ~ 32 DEG C of conditions;
(7) be separated: be separated after fermentation ends, obtain acetic acid fermentation liquid;
(8) spray: to the solid spray be separated, acetic acid and other functional activity materials are leached as far as possible, and merging spray liquid and zymotic fluid obtain acetate solution;
(9) merge: merge milk and acetic acid fermentation liquid;
(10) lactic fermentation: inoculating lactic acid bacterium, inoculum concentration 10%, standing for fermentation 24h under 28 ~ 32 DEG C of conditions;
(11) filter: adopt diatomite filtration;
(12) sterilization: carry out high-temperature short-time sterilization after filtration, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(13) ageing: ageing 20 days under normal temperature condition;
(14) allocate: the total acid content 2.0% adjusting above-mentioned beverage, and add 1.0% sucrose, 0.1% citric acid, 0.15% calcium lactate, 1% xanthans, 0.01% sodium Diacetate;
(15) homogeneous: deployed beverage is placed in homogenizer, at homogenization pressure 25MPa, homogeneous at temperature 60 ~ 70 DEG C, makes various nutritional labeling homogenising, prevents product from occurring layering and precipitating, product stability is further ensured;
(16) essence filter: the functional health vinegar beverage after homogeneous is first filtered through 200 order duplex strainers, then through the filter of diatom filter essence with removing impurity wherein and not tolerant;
(17) re-pasteurization: deployed functional health vinegar beverage carries out high-temperature short-time sterilization, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(18) filling: adopt vacuum filling, vacuum is 0.04-0.05Mpa, and filling temperature controls at 60-75 DEG C, then is cooled to normal temperature and gets product.
embodiment 2:
A kind of Chinese wolfberry fruit milk vinegar health drink, its concrete production technology is as follows:
(1) get the raw materials ready: get the raw materials ready according to matrimony vine 55 parts, 35 parts, milk, STEVIA REBAUDIANA 20 parts, wilsonii 12 parts, Radix Codonopsis 4 parts of ratios, remove infested raw material;
(2) Chinese herbal medicine lixiviate: after soaking 12h with 8 times of pure water, under 60 ~ 70 DEG C of conditions, with ultrasonic extractor process 20min, the active ingredient in the Chinese herbal medicines such as matrimony vine is fully leached in pure water;
(3) enzymolysis: add pectase, carbohydrase, cellulase respectively in 0.2g/100mL, 0.4g/100mL, 0.4g/100mL ratio, then at 40 ~ 60 DEG C, coordinated enzymatic hydrolysis 2 ~ 3h under stirring condition;
(4) prepare burden: add 0.15% ammonium sulfate, 0.08% potassium dihydrogen phosphate, 0.03% magnesium sulfate;
(5) alcoholic fermentation: regulate initial total sugar degree 13 ~ 15BX after enzymolysis, inoculation saccharomyces cerevisiae, inoculum concentration 6%, then carries out alcoholic fermentation (supernatant liquid stirs fermentation, lower floor's solid standing for fermentation, and 12h stirs once), at 20 ~ 28 DEG C of condition bottom fermentation 48h;
(6) acetic fermentation: inoculate acetic acid bacteria after alcoholic fermentation, inoculum concentration 10%, standing for fermentation 72h under 28 ~ 32 DEG C of conditions;
(7) be separated: be separated after fermentation ends, obtain acetic acid fermentation liquid;
(8) spray: to the solid spray be separated, acetic acid and other functional activity materials are leached as far as possible, and merging spray liquid and zymotic fluid obtain acetate solution;
(9) merge: merge milk and acetic acid fermentation liquid;
(10) lactic fermentation: inoculating lactic acid bacterium, inoculum concentration 6%, standing for fermentation 36h under 28 ~ 32 DEG C of conditions;
(11) filter: adopt diatomite filtration;
(12) sterilization: carry out high-temperature short-time sterilization after filtration, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(13) ageing: ageing 30 days under normal temperature condition;
(14) allocate: the total acid content 1.0% adjusting above-mentioned beverage, and add 1.5% xylitol, 0.1% citric acid, 0.02% calcium lactate, 2% xanthans, 0.02% sodium Diacetate;
(15) homogeneous: deployed beverage is placed in homogenizer, at homogenization pressure 25MPa, homogeneous at temperature 60 ~ 70 DEG C, makes various nutritional labeling homogenising, prevents product from occurring layering and precipitating, product stability is further ensured;
(16) essence filter: the functional health vinegar beverage after homogeneous is first filtered through 200 order duplex strainers, then through the filter of diatom filter essence with removing impurity wherein and not tolerant;
(17) re-pasteurization: deployed functional health vinegar beverage carries out high-temperature short-time sterilization, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(18) filling: adopt vacuum filling, vacuum is 0.04-0.05Mpa, and filling temperature controls at 60-75 DEG C, then is cooled to normal temperature and gets product.
embodiment 3:
A kind of Chinese wolfberry fruit milk vinegar health drink, its concrete production technology is as follows:
(1) get the raw materials ready: get the raw materials ready according to matrimony vine 50 parts, 30 parts, milk, STEVIA REBAUDIANA 15 parts, wilsonii 10 parts, Radix Codonopsis 5 parts of ratios, remove infested raw material;
(2) Chinese herbal medicine lixiviate: after soaking 12h with 9 times of pure water, under 60 ~ 70 DEG C of conditions, with ultrasonic extractor process 20min, the active ingredient in the Chinese herbal medicines such as matrimony vine is fully leached in pure water;
(3) enzymolysis: add pectase, carbohydrase, cellulase respectively in 0.15g/100mL, 0.6g/100mL, 0.3g/100mL ratio, then at 40 ~ 60 DEG C, coordinated enzymatic hydrolysis 2 ~ 3h under stirring condition;
(4) prepare burden: add 0.18% ammonium sulfate, 0.07% potassium dihydrogen phosphate, 0.025% magnesium sulfate;
(5) alcoholic fermentation: regulate initial total sugar degree 13 ~ 15BX after enzymolysis, inoculation saccharomyces cerevisiae, inoculum concentration 7%, then carries out alcoholic fermentation (supernatant liquid stirs fermentation, lower floor's solid standing for fermentation, and 12h stirs once), at 20 ~ 28 DEG C of condition bottom fermentation 42h;
(6) acetic fermentation: inoculate acetic acid bacteria after alcoholic fermentation, inoculum concentration 9%, standing for fermentation 84h under 28 ~ 32 DEG C of conditions;
(7) be separated: be separated after fermentation ends, obtain acetic acid fermentation liquid;
(8) spray: to the solid spray be separated, acetic acid and other functional activity materials are leached as far as possible, and merging spray liquid and zymotic fluid obtain acetate solution;
(9) merge: merge milk and acetic acid fermentation liquid;
(10) lactic fermentation: inoculating lactic acid bacterium, inoculum concentration 8%, standing for fermentation 30h under 28 ~ 32 DEG C of conditions;
(11) filter: adopt diatomite filtration;
(12) sterilization: carry out high-temperature short-time sterilization after filtration, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(13) ageing: ageing 25 days under normal temperature condition;
(14) allocate: the total acid content 1.5% adjusting above-mentioned beverage, and add 1.3% honey, 0.1% citric acid, 0.18% calcium lactate, 1.5% xanthans, 0.015% sodium Diacetate;
(15) homogeneous: deployed beverage is placed in homogenizer, at homogenization pressure 25MPa, homogeneous at temperature 60 ~ 70 DEG C, makes various nutritional labeling homogenising, prevents product from occurring layering and precipitating, product stability is further ensured;
(16) essence filter: the functional health vinegar beverage after homogeneous is first filtered through 200 order duplex strainers, then through the filter of diatom filter essence with removing impurity wherein and not tolerant;
(17) re-pasteurization: deployed functional health vinegar beverage carries out high-temperature short-time sterilization, sterilising temp is 120 ~ 140 DEG C, and the time is 4 ~ 6s;
(18) filling: adopt vacuum filling, vacuum is 0.04-0.05Mpa, and filling temperature controls at 60-75 DEG C, then is cooled to normal temperature and gets product.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.