CN104270966A - Antifungal agent - Google Patents
Antifungal agent Download PDFInfo
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- CN104270966A CN104270966A CN201380021987.9A CN201380021987A CN104270966A CN 104270966 A CN104270966 A CN 104270966A CN 201380021987 A CN201380021987 A CN 201380021987A CN 104270966 A CN104270966 A CN 104270966A
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- emblic
- fatty acid
- plant
- diet product
- acid ester
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/349—Organic compounds containing oxygen with singly-bound oxygen
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
- A23L3/3517—Carboxylic acid esters
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- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
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Abstract
The present invention pertains to an antifungal agent that can effectively inhibit proliferation of microorganisms that contribute to contamination of food and beverages or food and beverage products, particularly mold, yeast, and other fungi. In further detail, the present invention pertains to an antifungal agent containing amura plant or processed material thereof, fatty acid esters, and, if desired, hops extract.
Description
The reference of related application
Present patent application advocates the priority of No. 2012-247651, the Japanese patent application of No. 2012-104147, Japanese patent application and the submission on November 9th, 2012 submitted to based on April 27th, 2012, and whole disclosures of being somebody's turn to do in first patent application are considered as the part of this description by reference.
Technical field
The present invention relates to the antiseptic used in the sterilizing of the objects such as diet product.
Background technology
All the time, in order to suppress and the corruption during manufacture of processing food or in preserving, the propagation of deteriorated relevant bacterium, generation, the various antiseptics of the composition comprising various composite or natural goods source are proposed.
In addition, because relieved, the awareness of safety of food in recent years improve, the material in more preference natural goods source compared with composite, based on such background, commercially available have the antiseptic comprising natural goods origin components in a large number.
For example, Patent Document 1 discloses and be used as the ε-poly-D-lysine of natural goods, nucleoprotamine, food preservative agent mould etc. effectively to antibiotic property.
The antiseptic comprising these natural goods origin components shows effect to a certain degree for the fungi such as mould, yeast.But the composition in natural goods source has unique fragrance, taste more.Therefore, it is possible to the amount of adding in food is limited, when considering the affecting of flavour of food products, also existing and being difficult to give food sufficient conservatory problem.
Therefore, also studied natural goods source composition and various composite and use.
Such as, Patent Document 2 discloses a kind of food preservative agent with antibiotic property, it is characterized in that, and used polyglyceryl fatty acid ester and ε-poly-D-lysine.In addition, Patent Document 3 discloses a kind of food preservative agent with antibiotic property, it is characterized in that, and used polyglyceryl fatty acid ester and nucleoprotamine.In addition, Patent Document 4 discloses a kind of and used the antifungal agent of lecithin and food hydrophilic emulsifier.
But, generally speaking, almost not for the effective antifungal agent of both Molds and yeasts.For example, Patent Document 1 discloses: the nucleoprotamine recorded in the ε-poly-D-lysine recorded in patent document 2 and patent document 3 is when being used alone, low to the effect of fungi.In addition, in patent document 4, general fungi carries out sterilizing by heating.
Therefore, still seeking to play excellent effect to the fungi such as mould, yeast and the antifungal agent that utilize natural goods composition little on the impact of the local flavor of diet product etc.
Prior art document
Patent document
Patent document 1: Japanese Unexamined Patent Publication 2-107175 publication
Patent document 2: Japanese Unexamined Patent Publication 6-253797 publication
Patent document 3: Japanese Unexamined Patent Publication 2-23855 publication
Patent document 4: Japanese Unexamined Patent Publication 2000-197471 publication
Summary of the invention
What the present inventor carried out furtheing investigate found that, if by emblic plant or its handled thing and fatty acid ester Combination application in objects such as diet product, then can play excellent antibacterial effect to the fungi such as mould, yeast, by further by lupulus extract Combination application in emblic plant or its handled thing and fatty acid ester, then can play significant antibacterial effect.The present invention is based on this discovery.
Therefore, the object of the present invention is to provide the antifungal agent of bacterium, the particularly propagation of the fungi such as mould, yeast effectively suppressing relevant with the pollution of the object such as diet product.
Specifically, following invention is provided.
(1) antifungal agent, it comprises emblic plant or its handled thing and fatty acid ester.
(2) antifungal agent as described in (1), wherein, also comprises ethanol.
(3) antifungal agent as described in (1) or (2), wherein, also comprises lupulus extract.
(4) reduce a manufacture method for the diet product of the propagation risk of fungi, it is included in diet product and adds emblic plant or its handled thing and fatty acid ester.
(5) manufacture method of the diet product as described in (4), wherein, also comprises interpolation ethanol.
(6) manufacture method of the diet product as described in (4) or (5), wherein, also comprises interpolation lupulus extract.
(7) suppress a method for the propagation of the fungi in diet product, it is included in the plant or its handled thing and fatty acid ester that add emblic in diet product.
(8) method of the propagation of the fungi in the suppression diet product as described in (7), wherein, is also included in diet product and adds lupulus extract.
(9) combination of the plant containing emblic or its handled thing and fatty acid ester is as the application of antiseptic.
(10) application as described in (9), wherein, combinations thereof is also containing lupulus extract.
According to the present invention, the propagation of various mushrooms, the particularly fungi such as mould, yeast remained in the objects such as diet product effectively can be suppressed.In addition, antiseptic of the present invention is little from the viewpoint of the impact of the local flavor on diet product etc. is also favourable.
Detailed description of the invention
The feature of antifungal agent of the present invention is, it comprises emblic plant or its handled thing and fatty acid ester.Antifungal agent of the present invention can be advantageously used in the propagation suppressing to cause the spoilage organisms, particularly mould or the yeast that produce the deteriorations such as rotten or foreign odor by acting on object.
Antifungal agent of the present invention can be used in the fungi such as mould, yeast, as preferred fungi example, can enumerate: Cladosporium (
cladosporium?
cladosporioidesdeng), aspergillus (
aspergillus?
nigerdeng), Penicillium (
penicillium?
glabrumdeng) etc. mould, pichia (
pichia?
anomaladeng), saccharomyces (
saccharomyces?
cerevisiaedeng) etc. yeast.
About the emblic used in the present invention (Amla), being formal name used at school is
phyllanthus?
embilica, another name be
emblica?
officinalis?
gaertn, English by name
emblicmyrobalan, Japanese is called the deciduous tree (in low wood) of the Euphorbiaceae Leafflower of コ ミ カ Application ソ ウ, ユ カ Application, イ Application De ス グ リ or ア Application マ ロ Network etc.
Antifungal agent of the present invention can directly apply emblic plant, also can apply the various handled things of emblic plant.
As emblic plant, can enumerate such as: flower, fruit, leaf, stem, root, xylem, be preferably emblic fruit.At this, emblic fruit refers to the fruit with pulp that the tree of emblic grows.The emblic fruit used in the present invention is not particularly limited, and can be any one in immature fruit, full maturity fruit.
In addition, as the handled thing of the plant of emblic, can enumerate the various handled things obtained by carrying out physical treatment, chemical treatment or biological treatment to emblic plant, more specifically, be squeezing handled thing, dry handled thing, pulverization process thing or extraction process thing.
The process of the plant of emblic can by using known device squeezing to be processed, dryly to process (dry, air-dry, freeze drying etc.), pulverization process or extraction process etc. and to be used alone or multiple combination makes for carrying out.
In addition, extraction process can use normally used various extraction process in drink food processing field etc.As the concrete example of extraction process, can enumerate: solvent extraction, air-flow are extracted, squeezing is extracted, and solvent extraction or squeezing are extracted easy, thus preferably use.In addition, extraction process thing can implement Separation of Solid and Liquid process (absorption, filtration, centrifugation etc.), concentration, purification process etc. as required further.In addition, extraction process thing can be used in again extracting operation after once extracting operation.The concrete conditioned disjunction method extracting operation suitably can be selected according to the kind of emblic plant or amount.
As the Extraction solvent used in extraction process, from the view point of in middle utilizations such as diet product, preferably use the mixed solvent of the arbitrary ratio of water, ethanol or water and ethanol.
About the concentration of alcohol of mixed solvent, preferred alcohol concentration is 1 ~ 99.9 capacity %, is more preferably 2 ~ 60 capacity %, more preferably 5 ~ 30 capacity %.
As the concrete example of extraction operation, can enumerate and emblic fruit flooded in Extraction solvent at 0 ~ 50 DEG C and carries out after stirring 1 minute ~ 24 hours filtering or the method for centrifugation.At this, the condition such as temperature, time of extraction is not particularly limited, and those skilled in the art can suitably select according to the position of emblic plant, amount and set.
The handled thing of emblic plant of the present invention can obtain by using aforesaid operations, also can use commercially available product.As the handled thing of emblic plant, such as, can use dry thing, also can use pastel etc.It should be noted that, emblic fruit is carried out squeezing process and the fruit juice that obtains and the extract being got for water extraction by emblic fruit and obtain is commercially available dried powder, can easily obtain.
About the content of the emblic plant in antifungal agent of the present invention or its handled thing, with antifungal agent gross weight for benchmark, preferably count 0.1 ~ 2 % by weight with dry weight, be more preferably 0.2 ~ 2 % by weight, more preferably 0.2 ~ 1 % by weight, be particularly preferably 0.25 ~ 1 % by weight.This content is particularly advantageous when emblic plant or its handled thing are the water extract of phyllanthus emblica L.juice or emblic fruit.
In addition, fatty acid ester of the present invention is not particularly limited, preferably be selected from least one in the group be made up of sucrose fatty ester, mono-fatty acid glyceride, sorbitan fatty acid ester and polyglyceryl fatty acid ester, be more preferably sucrose fatty ester or mono-fatty acid glyceride.In addition, if consider the antibacterial effect improved fungi, then the aliphatic acid in sucrose fatty ester is preferably the aliphatic acid of carbon number 12 ~ 16, and the aliphatic acid in mono-fatty acid glyceride is preferably the aliphatic acid of carbon number 8 ~ 12.In addition, suppress when adding in diet product for low-level the impact of local flavor if consider, then fatty acid ester of the present invention is particularly preferably Sucrose myristate.
About the content of the fatty acid ester in antifungal agent of the present invention, with antifungal agent gross weight for benchmark, preferably count 0.05 ~ 1 % by weight with dry weight, be more preferably 0.1 ~ 1 % by weight.
Emblic plant in antifungal agent of the present invention or the weight ratio of its handled thing and fatty acid ester are not particularly limited, take dry weight as benchmark, such as relative to emblic plant 1 weight portion, preferably fatty acid ester is adjusted to 0.025 weight portion ~ 10 weight portion, is more preferably 0.025 weight portion ~ 5 weight portion, more preferably 0.1 weight portion ~ 5 weight portion, be particularly preferably 0.5 weight portion ~ 4 weight portion.
If consider the raising of antibiotic property, then antifungal agent of the present invention is preferably also containing aqueous organic solvent such as ethanol.The content of the ethanol in antifungal agent of the present invention is not particularly limited, and is preferably 30 ~ 90 % by weight, is more preferably 40 ~ 80 % by weight.
Antifungal agent of the present invention preferably comprises lupulus extract.In the present invention, " lupulus extract " refers to the extract of hops globe daisy (Balls flower), lupulus extract is carried out isomerization process and the isomerized lupulus extract obtained, the implication of reduced form isomerized lupulus extract that uses reducing agent different α acid reduction to be obtained use to comprise.
Containing the acidic resins composition such as α acid (humulone class), β acid (golden hop ketone) in lupulus extract.In addition, containing acidic resins compositions such as different α acid (Iso Humulon) in the lupulus extract after isomerization.In the present invention, " α acid, humulone class " is to comprise the implication use of humulone, companion humulone, class humulone, Posthumulone and front humulone.In addition, in the present invention, " β acid, golden hop ketone " is to comprise the implication use of lupulones, companion lupulones, class lupulones, rear lupulones and front lupulones.In addition, in the present invention, " different α acid, Iso Humulon " is to comprise the implication use of isohumulone, isoadohumulone, isocohumulone, different Posthumulone, isoprehumulone, Rho-isohumulone, Rho-isoadohumulone, Rho-isocohumulone, the different Posthumulone of Rho-, Rho-isoprehumulone, tetrahydro-iso-humulone, tetrahydrochysene isoadohumulone, tetrahydrochysene isocohumulone, tetrahydrochysene isoprehumulone, the different Posthumulone of tetrahydrochysene, Hexahydroisohumulone, six hydrogen isoadohumulones, six hydrogen isocohumulones, the different Posthumulone of six hydrogen, six hydrogen isoprehumulones.It should be noted that in Iso Humulon, to there is cis and transstereosiomer, but unless otherwise specified, then use with the implication comprised both this.According to a mode of the present invention, lupulus extract is preferably containing the α acid of 10 ~ 60 % by weight, the β acid of 10 ~ 60 % by weight.
The kind of hops is not particularly limited, any one kind can be used, can enumerate such as: than logical sequence (Bullion), gold makes (Brewers Gold), Ka Sikaide (Cascade), strange Nuck (Chinook), Green special this (Cluster), east Kent Ge Erding (East Kent Golding), Fu Geer (Fuggles), Harrar Dao Er (hallertau), Mount Hood (Mount Hood), (Northan Brewer) is made in north, Pei Le (Perle), Sa Zi (Saaz), Si Dilaien (Styrian), safe special nanmu victory (Tettnanger) and William Mai Te (Willamette) etc.
Lupulus extract of the present invention can be prepared by extracting natural hops, also can use commercially available lupulus extract.Hops extracting method of the present invention is not particularly limited, such as can enumerate: hot water extracting, water extraction are got, the extraction of steam extraction, use ethanol or acetone and other organic solvent or their mixture, use the extraction of the aqueous solution, use the means of supercritical extraction etc. of carbon dioxide etc., be preferably the means of supercritical extraction using carbon dioxide.
In addition, the extract obtained by said method can be directly used in the present invention, also can use the dilution of extract, also can use the concentrate of extract, can also use the dilution of concentrate.According to preferred embodiment of the present invention, lupulus extract is the concentrate of extract or the dilution of concentrate that are obtained by said method.
The method for concentration of the extract obtained by said method is not particularly limited, such as, can enumerate: reduced pressure concentration, distillation, ultrafiltration etc., is preferably reduced pressure concentration.The content of the lupulus extract in antifungal agent of the present invention is not particularly limited, and is preferably 0.025 ~ 0.5 % by weight, is more preferably 0.025 ~ 0.05 % by weight.
The solvent used in dilution etc. are not particularly limited, and can use known solvent.In addition, when diluting, considering deliquescent raising, can surfactant etc. be used.
Antifungal agent of the present invention can also contain pharmaceutically or the additive etc. that food hygiene can allow except above-mentioned points.As this additive, be not particularly limited, can enumerate such as: preserve material, antioxidant, thickening stabilizing agent, emulsifying agent, food fiber, pH adjusting agent etc.
In addition, antifungal agent of the present invention can contain chelating agent.Chelating agent is not particularly limited, and can enumerate such as: the organic acids such as acetic acid, citric acid, tartaric acid, malic acid, phosphoric acid, lactic acid, gluconic acid, phytic acid, is preferably citric acid.
As mentioned above, antifungal agent of the present invention can regulate using the plant containing emblic or its handled thing and fatty acid ester as the form of the composition of active ingredient.But, if the result that the plant of emblic or its handled thing and fatty acid ester use separately coexists, then also can use respectively.Therefore, according to another way of the present invention, provide a kind of antimycotic compound artifact, these goods comprise emblic plant or its handled thing and fatty acid ester, and by they while, successively or respectively add in object.In addition, according to another preferred embodiment of the present invention, provide a kind of antimycotic compound artifact, these goods comprise emblic plant or its handled thing, fatty acid ester and lupulus extract, and by they while, successively or respectively add in object.In addition, according to another way, provide the combination of the plant of emblic or its handled thing and fatty acid ester as the application of antibacterial agent.In addition, according to another preferred embodiment, provide the combination of the plant of emblic or its handled thing, fatty acid ester and lupulus extract as the application of antibacterial agent.The use amount of compound artifact of the present invention and using method can set according to the use amount in antifungal agent of the present invention and using method.
Antifungal agent of the present invention can manufacture by the mentioned component being representative with emblic plant or its handled thing, fatty acid ester and lupulus extract and other compositions are carried out suitably mixing with known method according to its prescription form.Therefore, according to a mode of the present invention, can provide a kind of manufacture method of antifungal agent, it comprises and emblic plant or its handled thing being mixed with fatty acid ester.In addition, according to above-mentioned preferred embodiment, the manufacture method of antifungal agent also comprises dog's-nose flower extract.In addition, according to another way, provide emblic plant or its handled thing and the application of fatty acid ester in the manufacture method of antifungal agent.In addition, according to another preferred embodiment, provide emblic plant or the application in the manufacture method of antifungal agent of its handled thing, fatty acid ester and lupulus extract.
In addition, antifungal agent of the present invention, by adding in the diet product as object, can suppress the propagation of the spoilage organisms of diet product, the particularly fungi such as mould, yeast.Therefore, according to the present invention, provide a kind of manufacture method reducing the diet product of the propagation risk of fungi, it comprises and emblic plant or its handled thing and fatty acid ester being added in diet product.In addition, according to preferred embodiment of the present invention, there is provided a kind of manufacture method reducing the diet product of the propagation risk of fungi, it comprises and regulates the concentration of the emblic plant in diet product or its handled thing and the concentration regulating emblic plant in diet product or its handled thing.In this manufacture method, emblic plant or its handled thing and fatty acid ester can be adjusted to effective amount for the propagation of Antifungi.Specifically, a kind of manufacture method reducing the diet product of the propagation risk of fungi is provided, it is characterized in that, preferably the concentration of the emblic plant in diet product or its handled thing is adjusted to 0.001 ~ 0.1 % by weight in dry weight, be more preferably 0.002 ~ 0.1 % by weight, more preferably 0.0025 ~ 0.05 % by weight, and preferably the relative concentration of the fatty acid ester in diet product is adjusted to 0.025 weight portion ~ 10 weight portion in emblic plant 1 weight portion, be more preferably 0.025 weight portion ~ 5 weight portion, more preferably 0.1 weight portion ~ 5 weight portion, be particularly preferably 0.5 weight portion ~ 4 weight portion.
In addition, according to more preferably mode of the present invention, the above-mentioned manufacture method reducing the diet product of the propagation risk of fungi also comprises adds to lupulus extract in diet product.In this manufacture method, the relative concentration of the lupulus extract in diet product is such as adjusted to 0.025 weight portion ~ 10 weight portion in emblic plant 1 weight portion, is preferably 0.025 weight portion ~ 5 weight portion, is more preferably 0.025 weight portion ~ 1 weight portion, more preferably 0.025 weight portion ~ 0.5 weight portion.
About " adjustment " of the emblic plant in diet product or the concentration of its handled thing or the concentration of fatty acid ester, emblic plant contained by can considering in former diet product originally or the concentration of its handled thing and fatty acid ester and in former diet product, add emblic plant or its handled thing and fatty acid ester regulate, also emblic plant or its handled thing can be added, or any one in fatty acid ester regulates, emblic plant or its handled thing can not also be added, any one material ground in fatty acid ester regulates.In addition, also can remove emblic plant or its handled thing and/or fatty acid ester from former diet product to regulate.Typically, emblic plant or its handled thing and fatty acid ester can be added in former diet product to regulate.
In addition, about the interpolation of emblic plant or its handled thing and/or fatty acid ester, emblic plant or its handled thing and/or fatty acid ester can add in the manufacture of former diet product or after the manufacture of former diet product.Emblic plant or its handled thing and fatty acid ester can together with add, also can adding respectively, when adding respectively, can first add any one material.When add multiple emblic plant or its handled thing, add multiple fatty acid ester when, all each composition can being added together, also can add respectively, when adding respectively, can first add any one material.In addition, self-evidently, when adding emblic plant or its handled thing and fatty acid ester, can consider that the concentration of emblic plant contained by former diet product originally or its handled thing and fatty acid ester is to determine whether that needs carry out adding or determining addition.
In addition, when emblic plant or its handled thing and/or fatty acid ester are added in the objects such as diet product, as adding method, be not particularly limited, suitably can select the mixing in object, the method such as coating, spraying to subject surface.Such as when spraying antifungal agent of the present invention, its spray amount suitably can be selected according to the kind of the amount of active ingredient, object, and in the plane raw-material situations such as plate sea sedge, spray amount can be such as every 1cm
2for more than 0.005ml, be preferably more than 0.01ml, more preferably more than 0.025ml.
In addition, the interpolation in diet product etc. of the adjustment of the lupulus extract concentration in diet product and lupulus extract can be carried out according to emblic plant or its handled thing above-mentioned adding method relevant with fatty acid ester.
The object adding antifungal agent of the present invention is not particularly limited, and except diet product as above, also can be applied to the surface etc. of the food article such as pharmaceuticals or cleaning implement, tableware, desk.But application is preferably diet product or pharmaceutical products, be more preferably diet product.Therefore, according to a mode of the present invention, provide the method for the propagation of a kind of fungi suppressed in diet product, it comprises and the plant of emblic or its handled thing and fatty acid ester being added in diet product.In addition, according to preferred embodiment of the present invention, the method for the propagation of above-mentioned Antifungi is also included in diet product adds lupulus extract.
Diet product of the present invention are not particularly limited, and can enumerate such as: the beverages etc. such as rice goods, soft canned food, fruit juice, soya-bean milk such as the Japanese dessert such as steamed bun, Western-style pastry such as bubble cottonrose hibiscus or steamed sponge cake etc., sushi or glutinous rice red bean meal.
Embodiment
Below, the present invention is further illustrated by embodiment.But the present invention is not limited to embodiment shown below.In addition, in following record, " % " expression " % by weight ".
Each substance migration following substances shown in following embodiment.
Emblic extract (powder): Inabata Koryo Co., Ltd..Carry out water extraction to the fruit of emblic to get and powdered and the material that obtains.
Phyllanthus emblica L.juice (powder): Inabata Koryo Co., Ltd..The fruit of emblic is squeezed the juice and powdered and the material that obtains.
Sucrose myristate: リ ョ ー ト ー シ ュ ガ ー エ ス テ Le M-1695 (Mitsubishi-kagaku Foods Corp.)
Surfhope SE Cosme C 1216: リ ョ ー ト ー シ ュ ガ ー エ ス テ Le L-1695 (Mitsubishi-kagaku Foods Corp.)
Sucrose palmitate: リ ョ ー ト ー シ ュ ガ ー エ ス テ Le P-1670 (Mitsubishi-kagaku Foods Corp.)
Green-tea extract: GUARDIAN green-tea extract: Danisco Co., Ltd.
Fatty acid glyceride (C10): シ ン コ ー ス ー パ ー 10 (new light science and technology (シ ン コ ー サ イ エ Application ス) Co., Ltd.)
Yucca extract: サ ラ キ ー プ ALS (Maruzen Pharmaceuticals Co., Ltd.).The mode reaching 0.5% with yucca extract content uses with after 57.2% ethanol dilution.
embodiment 1: the Proliferation Ability of yeast is tested (with comparing of fatty acid ester)
In ordinary broth fluid nutrient medium inoculation yeast (
pichia?
anomala) as thalline, make it reach about 10
3individual/ml.This suspension 5ml is placed in L shape pipe, adds the preparation 100 μ l of following composition, implement proliferation test.It should be noted that, preparation carries out metre filter process after by whole composition mixing.
In test, use バ イ オ Off ォ ト レ コ ー ダ ー TVS062CA (ADVANTEC) shaken cultivation at 30 DEG C, investigate propagation by the rising of absorbance (OD value 660nm).
The composition of preparation and result are shown in table 1, table 2 and table 3.Result represents with the absorbance (OD value: 660nm) of passing in time of nutrient solution, and numerical value is higher, then represent bacterium more propagation, fertility inhibition is lower.
[table 1]
[table 2]
[table 3]
As described in table 1 ~ 3, by will both the extract (emblic extract: carry out water extraction to the fruit of emblic and get and powdered and the material that obtains) of emblic fruit and fatty acid ester be derived from and use, compared with when they being used alone, demonstrate higher proliferation inhibiting effect.Particularly pass through and use emblic extract and Sucrose myristate or emblic extract and mono-fatty acid glyceride (C10), demonstrating high effect.
embodiment 2: the Proliferation Ability of yeast is tested (with comparing of polyphenoils)
In ordinary broth fluid nutrient medium inoculation yeast (
pichia?
anomala) as thalline, make it reach about 10
3individual/ml.This suspension 5ml is placed in L shape pipe, adds the preparation 100 μ l of following composition, implement proliferation test.It should be noted that, preparation carries out metre filter process after by whole composition mixing.In test, use バ イ オ Off ォ ト レ コ ー ダ ー TVS062CA (ADVANTEC) shaken cultivation at 30 DEG C, investigate propagation by the rising of absorbance (OD value 660nm).
Composition and the result of preparation are as shown in table 4.Result represents with the absorbance (OD value: 660nm) of passing in time of nutrient solution, and numerical value is higher, then represent bacterium more propagation, fertility inhibition is lower.
[table 4]
As described in Table 4, by by deriving from both the extract of emblic fruit and fatty acid ester and use, compared with other anti-bacterial materials such as green-tea extract, yucca extract, demonstrate higher proliferation inhibiting effect.
embodiment 3: the Proliferation Ability of yeast is tested (based on the extract deriving from emblic
the comparison of composition amounts)
In ordinary broth fluid nutrient medium inoculation yeast (
pichia?
anomala) as thalline, make it reach about 10
3individual/ml.This suspension 5ml is placed in L shape pipe, adds the preparation 100 μ l of following composition, implement proliferation test.It should be noted that, preparation carries out metre filter process after by whole composition mixing.Test is carried out similarly to Example 2.
The composition of preparation and result are as shown in Table 5 and 6.Result represents with the absorbance (OD value: 660nm) of passing in time of nutrient solution, and numerical value is higher, then represent bacterium more propagation, fertility inhibition is lower.
[table 5]
[table 6]
As shown in Table 5 and 6, by will both the extract of emblic fruit and fatty acid ester be derived from and use, compared with using the situation of the material of the source emblic of equivalent, using the situation of fatty acid ester, demonstrate higher proliferation inhibiting effect.
embodiment 4: the Proliferation Ability of yeast is tested (based on handled thing different of emblic
relatively)
In ordinary broth fluid nutrient medium inoculation yeast (
pichia?
anomala) as thalline, make it reach about 10
3individual/ml.This suspension 5ml is placed in L shape pipe, adds the preparation 100 μ l of following composition, implement proliferation test.It should be noted that, preparation carries out metre filter process after by whole composition mixing.Test is carried out similarly to Example 2.The composition of preparation and result are as shown in table 7 and table 8.Result represents with the absorbance (OD value: 660nm) of passing in time of nutrient solution, and numerical value is higher, then represent bacterium more propagation, fertility inhibition is lower.
[table 7]
[table 8]
As described in table 7 and 8, even if when the handled thing using the fruit juice of emblic as emblic, by with fatty acid ester and use, also demonstrate proliferation inhibiting effect higher compared with situation about they being used alone.
embodiment 5: the Proliferation Ability of mould is tested
Add in potato dextrose agar mould (
aspergillus?
niger) make it reach about 10
3cFU/ml, and be coated with equably.Cultivate after adding each preparation 500 μ l wherein, the growth after observing 30 DEG C, 3 days.Composition and the result of the preparation used are as shown in table 9.It should be noted that, result is evaluated with clump count (CFU/ml).
[table 9]
※ is without clump count when adding: 26CFU/ml
As described in Table 9, and used and derive from the extract of emblic fruit with the preparation of fatty acid ester compared with the preparation of comparative example 21 ~ 24, demonstrate higher fertility inhibition.
embodiment 6: food additive Accelerated Life (sea sedge volume)
Method: use water 200g, use common rice cooking machine to boil the rice of 150g.Mix sushi vinegar 26.5g (vinegar 20g, granulated sugar 5g, salt 1.5g) in rice after above-mentioned boiling, prepare vinegar meal.Divide in the culture dish after the sterilizing being added to φ 90mm by the vinegar meal being cooled to room temperature with every part of 30g, after surfacing is smooth, what mounting was cut into 60mm × 60mm thereon cures sea sedge.In this sea sedge surface coating as about 10 of thalline
4individual/ml ~ about 10
5the bacterium liquid 100 μ l of the yeast Pichia anomala of individual/ml.After the drying of bacterium liquid, spray the preparation 1ml of each trial zone shown in table 10 thereon, closes the lid and by sealed paper by after airtight for the surrounding of culture dish, preserve at 30 DEG C.Pass in time and sample, measurement viable count.It should be noted that, viable count is mean value when each trial zone is sampled to 2 times and measures.About the measuring method of viable count, relative to the total amount of sea sedge and rice, add the phosphate buffer (pH7.2) of 3 times amount, carry out pulverization process with homogenizer (ス ト マ ッ カ ー), prepare suspension.This suspension is coated in PDA agar medium, cultivate at 30 DEG C, measurement clump count.
[table 10]
Its result is as shown in table 11.
[table 11]
| Trial zone | 0th day | 2nd day | 6th day |
| Experimental example 13 | 3.4×10 2 | 1.9×10 3 | 5.1×10 4 |
| Comparative example 24 | 2.8×10 2 | 2.4×10 4 | 7.3×10 5 |
| Comparative example 25 | 1.0×10 2 | 2.4×10 3 | 1.3×10 6 |
| Comparative example 26 | 1.0×10 2 | 7.2×10 2 | 1.5×10 6 |
(unit: CFU/ml)
As described in Table 11, confirm the increase of bacterium number in preservation after the 2nd day, but pass through and inhibit propagation with the preparation that the extract and Sucrose myristate that derive from emblic fruit coordinate and obtain, compared with comparative example, effect is more excellent.In addition, spray on drink flavour of food products do not affect.
embodiment 7: the test containing hops
In ordinary broth fluid nutrient medium inoculation yeast (
pichia?
anomala) as thalline, make it reach about 10
3individual/ml.This suspension 5ml is placed in L shape pipe, adds the preparation 100 μ l of following composition, implement proliferation test.It should be noted that, preparation carries out metre filter process after by whole composition mixing.Test is carried out similarly to Example 2.Composition and the result of preparation are as shown in table 12.Result represents with the absorbance (OD value: 660nm) of passing in time of nutrient solution, and numerical value is higher, then represent bacterium more propagation, fertility inhibition is lower.
Lupulus extract uses the HPE CO utilizing the means of supercritical extraction of carbon dioxide
2extract (Hallertauer Hopfenveredelungs company).
[table 12]
[table 13]
As described in table 12 and table 13, by also containing lupulus extract, demonstrate inhibition and extend.
embodiment 8 food additive Accelerated Life
Use water 200g, use common rice cooking machine to boil the rice of 150g.Mix sushi vinegar 26.5g (vinegar 20g, granulated sugar 5g, salt 1.5g) in rice after above-mentioned boiling, prepare vinegar meal.Divide in the culture dish after the sterilizing being added to φ 90mm by the vinegar meal being cooled to room temperature with every part of 30g, after surfacing is smooth, what mounting was cut into 60mm × 60mm thereon cures sea sedge.In this sea sedge surface coating as about 10 of thalline
4individual/ml ~ about 10
5the yeast of individual/ml
pichia?
anomalabacterium liquid 100 μ l.After the drying of bacterium liquid, spray the preparation 1ml of each trial zone shown in table 10 and 12 thereon, closes the lid and by sealed paper by after airtight for the surrounding of culture dish, preserve at 30 DEG C.Pass in time and sample, measurement viable count.It should be noted that, viable count is mean value when each trial zone is sampled to 2 times and measures.About the measuring method of viable count, relative to the total amount of sea sedge and rice, add the phosphate buffer (pH7.2) of 3 times amount, carry out pulverization process with homogenizer (ス ト マ ッ カ ー), prepare suspension.This suspension is coated in PDA agar medium, cultivate at 30 DEG C, measurement clump count.
Its result is as shown in table 14.
[table 14]
| Trial zone | 0th day | 2nd day | 5th day |
| Experimental example 14 | 2.0×10 3 | 2.9×10 3 | 1.8×10 5 |
| Experimental example 15 | 8.2×10 2 | 1.8×10 3 | 2.5×10 4 |
| Comparative example 24 | 1.5×10 3 | 2.9×10 4 | 1.0×10 6 |
(unit: CFU/ml)
As described in Table 14, by and with deriving from the extract of emblic fruit and fatty acid glyceride and Sucrose myristate and also containing lupulus extract in the preparation coordinating and obtain, further suppress propagation.In addition, spray on drink flavour of food products do not affect.
Claims (8)
1. an antifungal agent, it comprises emblic plant or its handled thing and fatty acid ester.
2. antifungal agent as claimed in claim 1, wherein, also comprises ethanol.
3. antifungal agent as claimed in claim 1 or 2, wherein, also comprises lupulus extract.
4. reduce a manufacture method for the diet product of the propagation risk of fungi, it is included in diet product and adds emblic plant or its handled thing and fatty acid ester.
5. the manufacture method of diet product as claimed in claim 4, wherein, also comprises interpolation ethanol.
6. the manufacture method of the diet product as described in claim 4 or 5, wherein, also comprises interpolation lupulus extract.
7. suppress a method for the propagation of the fungi in diet product, it is included in the plant or its handled thing and fatty acid ester that add emblic in diet product.
8. the method for the propagation of the fungi suppressed in diet product as claimed in claim 7, wherein, is also included in diet product and adds lupulus extract.
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| Application Number | Priority Date | Filing Date | Title |
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| JP2012-104147 | 2012-04-27 | ||
| JP2012104147 | 2012-04-27 | ||
| JP2012247651 | 2012-11-09 | ||
| JP2012-247651 | 2012-11-09 | ||
| PCT/JP2013/062328 WO2013161977A1 (en) | 2012-04-27 | 2013-04-26 | Antifungal agent |
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|---|---|
| JP (1) | JP6181046B2 (en) |
| KR (1) | KR102236086B1 (en) |
| CN (1) | CN104270966B (en) |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1062640A (en) * | 1991-09-27 | 1992-07-15 | 宋心仿 | food preservative and preparation method thereof |
| JPH07147948A (en) * | 1993-12-02 | 1995-06-13 | Mitsubishi Chem Corp | 'shiruko' in sealed container |
| CN1870912A (en) * | 2003-09-09 | 2006-11-29 | 3M创新有限公司 | Concentrated antimicrobial compositions and methods |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0223855A (en) | 1988-07-11 | 1990-01-26 | Taiyo Kagaku Co Ltd | Preservation of food |
| JPH02107175A (en) | 1988-10-17 | 1990-04-19 | Okuno Seiyaku Kogyo Kk | Food preservative |
| JPH06253797A (en) | 1993-02-26 | 1994-09-13 | Taiyo Kagaku Co Ltd | Preservative for food |
| JP2000197471A (en) | 1998-08-10 | 2000-07-18 | Mitsubishi Kagaku Foods Kk | Antifungal agent and suppression of propagation of fugus |
| JP2005168430A (en) * | 2003-12-12 | 2005-06-30 | Yonekyu Corp | Keeping quality-improving agent for food and strictly anaerobic bacterium proliferation inhibitor |
| JP4455955B2 (en) * | 2004-08-20 | 2010-04-21 | 太陽化学株式会社 | Antioxidant composition |
| JP2006335709A (en) | 2005-06-03 | 2006-12-14 | Taiyo Kagaku Co Ltd | Tissue factor inhibition composition and food and drink, feed, quasi drug and drug containing the same, |
| WO2009060304A2 (en) | 2007-11-09 | 2009-05-14 | Avesthagen Limited | Emblica officinalis plant extracts and uses thereof |
-
2013
- 2013-04-26 KR KR1020147033218A patent/KR102236086B1/en active IP Right Grant
- 2013-04-26 CN CN201380021987.9A patent/CN104270966B/en not_active Expired - Fee Related
- 2013-04-26 WO PCT/JP2013/062328 patent/WO2013161977A1/en active Application Filing
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1062640A (en) * | 1991-09-27 | 1992-07-15 | 宋心仿 | food preservative and preparation method thereof |
| JPH07147948A (en) * | 1993-12-02 | 1995-06-13 | Mitsubishi Chem Corp | 'shiruko' in sealed container |
| CN1870912A (en) * | 2003-09-09 | 2006-11-29 | 3M创新有限公司 | Concentrated antimicrobial compositions and methods |
Non-Patent Citations (8)
| Title |
|---|
| 《全国中草药汇编》编写组: "《全国中草药汇编》", 31 December 1996, article ""啤酒花"" * |
| LIU, X., ET AL.: ""Effectiveness of Phyllanthus emblica L, essential oil to inhibit the growth of food-spoiling yeasts", 《JOURNAL OF FOOD SAFETY》, 31 December 2008 (2008-12-31), pages 261 - 275 * |
| 唐春红等: "余甘子果实粗提物的抑菌活性研究", 《食品科学》, no. 07, 1 April 2009 (2009-04-01) * |
| 张志健 等: "《食品防腐保鲜技术》", 31 January 2006 * |
| 王亦芸: "蔗糖酯的抗菌作用和抑制油脂败坏的效果", 《食品工业》, no. 03, 31 December 1995 (1995-12-31) * |
| 赵谋明等: "超临界CO_2萃取余甘子精油成分及精油抑菌活性", 《华南理工大学学报(自然科学版)》, no. 12, 15 December 2007 (2007-12-15) * |
| 陈顺天等: "从安摩乐科研成果看《本草纲目》的学术价值", 《海峡药学》, no. 03, 31 December 1996 (1996-12-31) * |
| 高彦祥: "《食品添加剂》", 31 May 2011 * |
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| JP6181046B2 (en) | 2017-08-16 |
| WO2013161977A1 (en) | 2013-10-31 |
| KR20150013618A (en) | 2015-02-05 |
| HK1201692A1 (en) | 2015-09-11 |
| JPWO2013161977A1 (en) | 2015-12-24 |
| CN104270966B (en) | 2017-10-03 |
| KR102236086B1 (en) | 2021-04-05 |
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