CN104257583B - Pearl enzymolysis solution and preparation method thereof - Google Patents
Pearl enzymolysis solution and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a pearl enzymolysis solution. The pearl enzymolysis solution comprises the raw materials, namely pearl powder, lactic acid, papaya peptidase, L-cysteine, ethylenediaminetetraacetic acid disodium salt and distilled water. The invention also discloses a method for preparing the pearl enzymolysis solution. The method comprises the following steps: (1) preparing a lactic acid solution; (2) carrying out enzymolysis reaction; and (3) centrifuging enzymolysis products and carrying out sterilization treatment on obtained supernate to obtain the pearl enzymolysis solution. By utilizing the method, the papaya peptidase is extracted from the papaya powder simply and conveniently with low cost and high purity, the pearl powder is subjected to enzymolysis by adopting the papaya peptidase, twenty amino acids and micro-molecule polypeptide substance with the molecular weight about 300-1000 can be obtained mainly, types of the pearl powder subjected to enzymolysis are enriched, enzymolysis of pearl can be carried out not only by using one single enzyme, and actual and efficient utilization of the pearl powder is further promoted.
Description
Technical field
The invention belongs to the technical field of biological enzymolysis, specifically a kind of Margarita enzymolysis solution and preparation method thereof.
Background technology
Margarita is a rarity in motherland's medicine treasure-house, early in " Compendium of Materia Medica " of " Mingyi Bielu " to the Ming Dynasty of Han dynasty
In have definite record to the curative effect of Margarita.For a long time people are always maintained at skin using the method for long-term taking Margarita powder
Lubrication is fine and smooth, but because composition main in Margarita powder is that Calcium Carbonate and some other composition are difficult to be absorbed by the body.It is particularly precious
Chitosan-alginate microcapsules matter in pearl is not easily decomposed in vivo absorption.Therefore the positive role and its specific function of Margarita powder are hindered significantly.
With the continuous development of science and technology, the continuous improvement of people's living standard also has to the exploitation of Margarita powder
New imagination, Margarita hydrolyzed solution, efficient Margarita powder etc. of country's report be only the Calcium Carbonate of indissoluble is made it is solvable organic
Calcium, substantially it is Calcium Carbonate and part trace element.And Chitosan-alginate microcapsules matter is not also utilized.And entitled " water solublity is complete
In the patent application edibility carboxylic acid of component pearl powder product series new preparation process " CN1051394A or amino acid replacement Margarita
The carbonate of mineral, separates conchiolin, then whole point of several water solublity is obtained with acid, the double Hydrolyze method of enzyme
Pearl products, but there is certain weak point.
Because Pearl fails by the easy composition for absorbing of complete hydrolysis adult's physical ability, therefore also exist at present certain
The Chitosan-alginate microcapsules matter of one of shortcoming, especially its main component also by human body is not fully absorbed and made full use of.In order to solve this
One technological deficiency, the patent of Application No. 94112053.8 is disclosed and is digested Margarita powder using papain, can obtain people
Aminoacid and polypeptide that body can absorb, but this method enzymolysis is not thoroughly, digests the polypeptide fragment molecular weight for obtaining greatly, in order to
Enzymolysis efficiency is improved, more small-molecular peptides products is obtained, through repetition test invention the method.
The content of the invention
In order to solve the technological deficiency described in background technology, the invention discloses a kind of enzymolysis Margarita liquid and preparation side
Method, present invention aim at finding that a kind of natural active products mass-energy makes Margarita powder digest aminoacid and the people that adult body easily absorbs
The beneficial little peptide molecule of body, makes Margarita powder be utilized completely, gives full play to its specific function, and breaking prior art can only adopt
The technical bottleneck of protease.
Realize above-mentioned purpose, the technical scheme for being adopted for, Margarita enzymolysis solution disclosed by the invention, the raw material of the enzymolysis solution
Including Margarita powder, volumetric concentration be 5～10% lactic acid solutions, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA,
Distilled water, the mass ratio of mentioned component is 15～30:50～100:10～15:1～3:1～3:1000.The present invention adopts Fructus Chaenomeliss
Peptidase is digested to the Chitosan-alginate microcapsules matter in Margarita, and the raw material for being adopted has no toxic side effect, and makes the product that enzymolysis is obtained
Without any toxicity, so that the Margarita enzymolysis solution broad range of applicability.
In addition, the invention also discloses the preparation method of the Margarita enzymolysis solution, comprises the steps, (1) prepares breast
Acid solution, the volumetric concentration of lactic acid solution is 5～10%；
(2) in the lactic acid solution for preparing to step (1) first add Margarita powder carry out acidolysis reaction, after acidolysis reaction from
The heart, the precipitate for obtaining is Margarita Chitosan-alginate microcapsules, then by Margarita Chitosan-alginate microcapsules, Fructus Chaenomeliss peptidase, L-Cysteine, ethylenediamine tetraacetic
Acetic acid diamine salts, distilled water mixing, carry out enzyme digestion reaction, Margarita powder, the lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, second
Edetate disodium salt, the mass ratio of distilled water are 15～30:50～100:10～15:1～3:1～3:1000, Fructus Chaenomeliss peptide
The aminoacid sequence of enzyme is such as SEQ ID NO:Sequence shown in 1；
(3) after enzyme digestion reaction, enzymatic hydrolysate is centrifuged, the supernatant for obtaining is carried out into sterilization treatment, that is, obtain treasure
Pearl enzymolysis solution.
The present invention is first by Margarita powder lactic acid treatment so as to become the calcium lactate liquid of solubility.Its main component is treasure
Pearl activated calcium and trace element, and insoluble part is pearl shell keratin protein, by pearl shell keratin protein natural active products
Matter Fructus Chaenomeliss titanium enzyme is hydrolyzed, and when enzymolysis enzyme is selected, papaya powder is usually the flour that papaya milk drying is obtained, wood
There are various enzymes in melon milk, mainly contain four kinds of cysteine enzymes:Papain, Fructus Chaenomeliss peptidase, chymopapain A
And B.In addition, lysozyme is also contained, II type chitinase and lipase etc., the aminoacid sequence of various enzymes is different, its life
Changing property also has very big difference.The present invention is finally digested Margarita powder using Fructus Chaenomeliss peptidase through test, makes to be difficult originally by people
Chitosan-alginate microcapsules become 20 kinds of aminoacid that human body easily absorbs in the Margarita powder of absorption, wherein there is eight kinds of amino needed by human
Acid, and peptide material, are 10.19mg/ml- by total amino acid content in the enzymolysis solution that the preparation method of the present invention is obtained
Absorption of the mixture of 22.82mg/ml, the micromolecule polypeptide also containing molecular weight about 300-1000 and aminoacid in body
Rate and speed are all good than simple aminoacid.And their biological value is higher than pure aminoacid, Margarita powder is significantly improved
Effective rate of utilization.
Preferably, the method for step (3) extraction Fructus Chaenomeliss peptidase is：1. papaya powder is dissolved in into 0.1M Acetic acid-sodium acetates to delay
In rushing solution；
2. ammonium sulfate is added in 1. step mixed liquor, the saturation for making mixed liquor reaches 0.45, places sucking filtration after 12-16h,
Supernatant fluid is dialysed with mass concentration 0.1MNaCl solution；
3. the dialysis solution upper prop CM-Sepharose for obtaining will be 2. walked, will be then 0.05-1.0M NaCl with mass concentration
Solution carries out gradient elution, collects the last liquid for flowing out；
4. ammonium sulfate is added until solution saturation reaches 0.85 to 3. walking the liquid that obtains, place sucking filtration after 12-16h,
The precipitation for obtaining is dialysed with mass concentration 0.05M NaCl solution；Dialysis solution is freeze-dried to obtain into Fructus Chaenomeliss peptide enzyme powder
End.Isolating and purifying for Fructus Chaenomeliss peptidase be not easy to operate, and the present invention selects the method to extract Fructus Chaenomeliss peptidase, and the purity of extraction is most
Height, operational approach is the easiest.
Preferably, Margarita powder, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA, distilled water in step (3)
Mass ratio be, selected quality proportioning under conditions of cost is minimum, enzymolysis it is the most abundant.
Preferably, hydrolysis temperature is 55～65 DEG C in step (2), and enzymolysis pH is 6～7, and enzymolysis time is 12～24h.Should
Parameter Conditions can just be such that Margarita powder fully digests as micromolecule polypeptide and aminoacid.
To sum up, the invention has the beneficial effects as follows, easy from papaya powder, low cost, extracted to high-purity Fructus Chaenomeliss peptide
Enzyme, and Fructus Chaenomeliss peptidase is a kind of natural bioactivity substance, and Margarita powder is digested using the peptidase, mainly can be obtained
20 kinds of aminoacid and molecular weight are about the micromolecule polypeptide material of 300-1000, and enzymatic hydrolyzation is high, and the aminoacid for obtaining
And peptide material can be good at being absorbed by the body, the effective rate of utilization of Margarita powder is significantly improved, and enrich enzymolysis
The species of Margarita powder, is digested a kind of enzyme of use that no longer can only be single when Margarita is digested, further promotes Margarita
Powder is really efficiently utilized.
Specific embodiment
Present invention is further illustrated below in conjunction with specific embodiment.Under without specified otherwise, operation
Method is adopted well known to a person skilled in the art method.
Embodiment one：A kind of Margarita enzymolysis solution that the present invention is developed, the raw material of the enzymolysis solution includes that Margarita powder, volume are dense
Spend for 5% lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA, distilled water, the quality of mentioned component
Than for 15:50:15:1:1:1000.
The raw material taken of the present invention has no toxic side effect, the enzymolysis solution for obtaining also without any toxicity, as health promoting beverage
Drink, cosmetics are applied to skin and are free from side effects, by enzymolysis Margarita liquid by Shanghai institute for drug control to medicine toxicity test method
Carry out mice lavage acute toxicity LD50Test.As a result it is as follows：
Using Kunming hybrid white mice, body weight is 20 grams, is divided into two groups of male and female.10 per group, same to dosed administration, experiment is seen
Examine seven days, with maximum tolerance every 1ml of gastric infusion, daily gavage is secondary, and after filling within continuous three days little Bai is observed for enzymolysis Margarita liquid
The hair color of Mus is smooth, and active state is good, and other belong to normal, draw 1ml/20 gram of acute toxicity mice lavage.White mice
Body weight pours into animal gastric equivalent to 50ml/kg liquid measures, death does not occur.This product belongs to nontoxic level.
Embodiment two：The raw material of the Margarita enzymolysis solution of the present invention includes that Margarita powder, volumetric concentration are 10% lactic acid solution, wood
Melon peptidase, L-Cysteine, disodium EDTA, distilled water, the mass ratio of mentioned component is 30:100:10:3:3:1000.
The method for preparing above-mentioned Margarita enzymolysis solution comprises the steps that (1) prepares lactic acid solution, and the volume of lactic acid solution is dense
Spend for 10%；
(2) in the lactic acid solution for preparing to step (1) first add Margarita powder carry out acidolysis reaction, after acidolysis reaction from
The heart, the precipitate for obtaining is Margarita Chitosan-alginate microcapsules, then by Margarita Chitosan-alginate microcapsules, Fructus Chaenomeliss peptidase, L-Cysteine, ethylenediamine tetraacetic
Acetic acid diamine salts, distilled water mixing, carry out enzyme digestion reaction, Margarita powder, the lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, second
Edetate disodium salt, the mass ratio of distilled water are 30:100:10:3:3:1000, the aminoacid sequence of Fructus Chaenomeliss peptidase be as
SEQ ID NO:Sequence shown in 1；
(3) after enzyme digestion reaction, enzymatic hydrolysate is centrifuged, the supernatant for obtaining is carried out into sterilization treatment, that is, obtain treasure
Pearl enzymolysis solution.
General to measure the total nitrogen content of Margarita enzymolysis solution using sulfosalicylic acid before sterilizing, concrete operation method is：
Taking enzymolysis Margarita liquid 0.2ml adds 6% sulfosalicylic acid, clear liquid to dilute 10 times, carry out amino acid analysises result such as
Under, the following is the content of the various aminoacid of every milliliter of Margarita enzymolysis solution：
| Amino acid name | Content | Amino acid name | Content |
| Aspartic Acid | 0.57mg/mL | Leucine | 1.40mg/mL |
| Threonine | 0.23mg/mL | L-Tyrosine | 1.40mg/mL |
| Serine | 1.16mg/mL | Phenylalanine | 2.87mg/mL |
| Glutamic acid | 0.31mg/mL | Ornithine | 0.47mg/mL |
| Proline | 0.08mg/mL | Histidine | 0.11mg/mL |
| Glycine | 3.63mg/mL | Lysine | 0.89mg/mL |
| Alanine | 2.51mg/mL | Tryptophan | 0.91mg/mL |
| L-Valine | 0.55mg/mL | Arginine | 2.03mg/mL |
| Methionine | 2.89mg/mL | Taurine | 0.68mg/mL |
| Isoleucine | 0.08mg/mL | Cystine | 0.05mg/mL |
Various total amino acid contents are per milliliter containing 22.82 milligrams.In addition to above-mentioned aminoacid, molecular weight is also mainly contained
About 300-1000 micromolecule polypeptides material.Beneficial to the human body aminoacid obtained Jing after enzymolysis processing and small peptide.
Embodiment three：The Margarita enzymolysis solution of present invention exploitation, the raw material of the enzymolysis solution includes that Margarita powder, volumetric concentration are
7% lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA, distilled water, the mass ratio of mentioned component is
20:70:13:2:2:1000。
The method for preparing above-mentioned Margarita enzymolysis solution comprises the steps that (1) prepares lactic acid solution, and the volume of lactic acid solution is dense
Spend for 9%；
(2) in the lactic acid solution for preparing to step (1) first add Margarita powder carry out acidolysis reaction, after acidolysis reaction from
The heart, the precipitate for obtaining is Margarita Chitosan-alginate microcapsules, then by Margarita Chitosan-alginate microcapsules, Fructus Chaenomeliss peptidase, L-Cysteine, ethylenediamine tetraacetic
Acetic acid diamine salts, distilled water mixing, carry out enzyme digestion reaction, Margarita powder, the lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, second
Edetate disodium salt, the mass ratio of distilled water are 20:70:13:2:21000, the aminoacid sequence of Fructus Chaenomeliss peptidase is such as SEQ
ID NO:Sequence shown in 1；
(3) after enzyme digestion reaction, enzymatic hydrolysate is centrifuged, the supernatant for obtaining is carried out into sterilization treatment, that is, obtain treasure
Pearl enzymolysis solution.
Step (2) extracts the method for Fructus Chaenomeliss peptidase：1. papaya powder is dissolved in 0.1M NaAc_HAc buffer solutions；
2. ammonium sulfate is added in 1. step mixed liquor, the saturation for making mixed liquor reaches 0.45, places sucking filtration after 12-16h,
Supernatant fluid is dialysed with mass concentration 0.1MNaCl solution；
3. the dialysis solution upper prop CM-Sepharose for obtaining will be 2. walked, will be then 0.05-1.0M NaCl with mass concentration
Solution carries out gradient elution, collects the last liquid for flowing out；
4. ammonium sulfate is added until solution saturation reaches 0.85 to 3. walking the liquid that obtains, place sucking filtration after 12-16h,
The precipitation for obtaining is dialysed with mass concentration 0.05M NaCl solution；Dialysis solution is freeze-dried to obtain into Fructus Chaenomeliss peptide enzyme powder.
The method of the extraction Fructus Chaenomeliss peptidase preferably adopted in the present embodiment, extraction ratio highest only includes below example, comes
Illustrate the test that selective extraction Fructus Chaenomeliss peptide enzyme method is carried out：
Select Margarita powder in step (2), lactic acid emulsion, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA,
During the quality ratio of distilled water, using mass ratio of the present invention under conditions of cost is minimum, percent hydrolysiss are higher, and residue is less,
Contrast test checking is as follows：
Hydrolysis temperature is 55～65 DEG C in step (2), and enzymolysis pH is 6～7, and enzymolysis time is 3～5h.The enzymolysis bar of selection
Part is all to ensure that hydrolysis can be completed under this condition, and coordinates above-mentioned quality proportioning, makes pearl shell keratin hydrolysis fully, from
And the polypeptide of more small molecule is obtained, promote absorption of human body.
General to measure the total nitrogen content of Margarita enzymolysis solution using sulfosalicylic acid before sterilizing, concrete operation method is：
Taking enzymolysis Margarita liquid 0.2ml adds 6% sulfosalicylic acid, clear liquid to dilute 10 times, carry out amino acid analysises, the following is
The content of the various aminoacid of every milliliter of Margarita enzymolysis solution：
| Amino acid name | Content | Amino acid name | Content |
| Aspartic Acid | 0.37mg | Leucine | 0.20mg |
| Threonine | 0.52mg | L-Tyrosine | 0.54mg |
| Serine | 0.22mg | Phenylalanine | 0.41mg |
| Glutamic acid | 0.42mg | Ornithine | 0.52mg |
| Proline | 0.32mg | Histidine | 0.33mg |
| Glycine | 0.30mg | Lysine | 0.22mg |
| Alanine | 0.37mg | Tryptophan | 0.57mg |
| L-Valine | 0.45mg | Arginine | 0.62mg |
| Methionine | 0.42mg | Taurine | 0.37mg |
| Isoleucine | 0.29mg | Cystine | 0.12mg |
Various total amino acid contents are per milliliter containing 7.58 milligrams.
In addition to above-mentioned aminoacid, also mainly contain molecular weight and be about 300-1000 micromolecule polypeptide materials.
Example IV：The Margarita enzymolysis solution of present invention exploitation, the raw material of the enzymolysis solution includes that Margarita powder, volumetric concentration are
9% lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA, distilled water, the mass ratio of mentioned component is
25:90:14:1.5:2.5:1000。
The method for preparing above-mentioned Margarita enzymolysis solution comprises the steps that (1) prepares lactic acid solution, and the volume of lactic acid solution is dense
Spend for 9%；
(2) in the lactic acid solution for preparing to step (1) first add Margarita powder carry out acidolysis reaction, after acidolysis reaction from
The heart, the precipitate for obtaining is Margarita Chitosan-alginate microcapsules, then by Margarita Chitosan-alginate microcapsules, Fructus Chaenomeliss peptidase, L-Cysteine, ethylenediamine tetraacetic
Acetic acid diamine salts, distilled water mixing, carry out enzyme digestion reaction, Margarita powder, the lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, second
Edetate disodium salt, the mass ratio of distilled water are 25:90:14:1.5:2.5:1000, the aminoacid sequence of Fructus Chaenomeliss peptidase is
Such as SEQ ID NO:Sequence shown in 1；
Step (3) extracts the method for Fructus Chaenomeliss peptidase：1. papaya powder is dissolved in into 0.1M NaAc_HAc buffer solutions
In；
2. ammonium sulfate is added in 1. step mixed liquor, the saturation for making mixed liquor reaches 0.45, places sucking filtration after 12-16h,
Supernatant fluid is dialysed with mass concentration 0.1MNaCl solution；
3. the dialysis solution upper prop CM-Sepharose for obtaining will be 2. walked, will be then 0.05-1.0M NaCl with mass concentration
Solution carries out gradient elution, collects the last liquid for flowing out；
4. ammonium sulfate is added until solution saturation reaches 0.85 to 3. walking the liquid that obtains, place sucking filtration after 12-16h,
The precipitation for obtaining is dialysed with mass concentration 0.05M NaCl solution；Dialysis solution is freeze-dried to obtain into Fructus Chaenomeliss peptide enzyme powder
End.
The method of the extraction Fructus Chaenomeliss peptidase preferably adopted in the present embodiment, extraction ratio highest only includes below example, comes
Illustrate the test that selective extraction Fructus Chaenomeliss peptide enzyme method is carried out：
Select Margarita powder in step (2), lactic acid emulsion, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA,
During the quality ratio of distilled water, using mass ratio of the present invention under conditions of cost is minimum, percent hydrolysiss are higher, and residue is less,
Contrast test checking is as follows：
Hydrolysis temperature is 55～65 DEG C in step (2), and enzymolysis pH is 6～7, and enzymolysis time is 3～5h.The enzymolysis bar of selection
Part is all to ensure that hydrolysis can be completed under this condition, and coordinates above-mentioned quality proportioning, makes pearl shell keratin hydrolysis fully, from
And the polypeptide of more small molecule is obtained, promote absorption of human body.
General to measure the total nitrogen content of Margarita enzymolysis solution using sulfosalicylic acid before sterilizing, concrete operation method is：
Taking enzymolysis Margarita liquid 0.2ml adds 6% sulfosalicylic acid, clear liquid to dilute 10 times, carry out amino acid analysises, the following is
The content of the various aminoacid of every milliliter of Margarita enzymolysis solution：
Various total amino acid contents are per milliliter containing 10.19 milligrams.In addition to above-mentioned aminoacid, molecular weight is also mainly contained
About 300-1000 micromolecule polypeptides material.Beneficial to the human body aminoacid obtained Jing after enzymolysis processing and small peptide.
The above restriction that not present invention is made, all any modifications made in flesh and blood of the present invention, etc.
With replacement and simple modifications etc., should be included within the scope of the present invention.
Claims (2)
1. a kind of Margarita enzymolysis solution, it is characterised in that the raw material of the enzymolysis solution includes that Margarita powder, volumetric concentration are 5～10% newborn
Acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA, distilled water, the mass ratio of mentioned component is 15～
30:50～100:10～15:1～3:1～3:1000.
2. a kind of method for preparing Margarita enzymolysis solution described in claim 1, it is characterised in that the method comprises the steps, (1)
Lactic acid solution is prepared, the volumetric concentration of lactic acid solution is 5～10%；(2) add in the lactic acid solution for preparing to step (1) first
Plus Margarita powder carries out acidolysis reaction, it is centrifuged after acidolysis reaction, the precipitate for obtaining is Margarita Chitosan-alginate microcapsules, then by pearl shell angle
Albumen, Fructus Chaenomeliss peptidase, L-Cysteine, ethylenediaminetetraacetic acid diamine salts, distilled water mixing, carry out enzyme digestion reaction, Margarita powder, institute
It is 15～30 to state lactic acid solution, Fructus Chaenomeliss peptidase, L-Cysteine, disodium EDTA, the mass ratio of distilled water:50～
100:10～15:1～3:1～3:1000, the aminoacid sequence of Fructus Chaenomeliss peptidase is such as SEQ ID NO:Sequence shown in 1；(3) enzyme
After solution reaction, enzymatic hydrolysate is centrifuged, the supernatant for obtaining is carried out into sterilization treatment, that is, obtain Margarita enzymolysis solution；
Wherein, the method for step (2) extraction Fructus Chaenomeliss peptidase is：1. papaya powder is dissolved in into 0.1M NaAc_HAc buffer solutions
In；2. ammonium sulfate is added in 1. step mixed liquor, the saturation for making mixed liquor reaches 0.45, places sucking filtration after 12-16h, will be upper
Clear liquid body is dialysed with mass concentration 0.1MNaCl solution；3. the dialysis solution upper prop CM-Sepharose for obtaining, Ran Houyong will 2. be walked
Mass concentration carries out gradient elution for 0.05-1.0MNaCl solution, collects the last liquid for flowing out；4. to 3. walking the liquid that obtains
Body adds ammonium sulfate to reach 0.85 up to solution saturation, sucking filtration after 12-16h is placed, by the precipitation mass concentration for obtaining
0.05M NaCl solutions are dialysed；Dialysis solution is freeze-dried to obtain into Fructus Chaenomeliss peptide enzyme powder；And hydrolysis temperature is 55～65 DEG C,
Enzymolysis pH is 6～7, and enzymolysis time is 12～24h.
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| CN105854002A (en) * | 2016-01-15 | 2016-08-17 | 上海荣神生物化学有限公司 | Polypeptide powder composition and tablet and preparation method thereof |
| CN105664140A (en) * | 2016-01-15 | 2016-06-15 | 上海荣神生物化学有限公司 | Glycopeptide composition as well as preparation method and application thereof |
| CN108822094A (en) * | 2018-09-17 | 2018-11-16 | 西安利君精华药业有限责任公司 | A kind of silymarin extraction process |
| CN110240627B (en) * | 2019-07-19 | 2023-06-27 | 浙江长生鸟健康科技股份有限公司 | Method for extracting small molecule active peptide from pearl powder by utilizing complex enzyme |
| CN110477381A (en) * | 2019-09-03 | 2019-11-22 | 广东紫薇星健康产业有限公司 | A kind of albumin amino acid Calcium oral liquid prescription and preparation method with immunological regulation healthcare function |
| CN111471731A (en) * | 2020-04-15 | 2020-07-31 | 南京立康生物科技有限公司 | Method for preparing enzymolysis pearl peptide |
| CN111514093A (en) * | 2020-05-18 | 2020-08-11 | 广西大海阳光药业有限公司 | Silver ion ethanol disinfectant and preparation method thereof |
| CN112294744A (en) * | 2020-10-28 | 2021-02-02 | 湖州欧利生物科技有限公司 | Preparation process of enzymolysis pearl freeze-dried powder |
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| Derermination of the structure of papaya protease omega;R. W. Pickersgill等;《Acta crystallographica section B》;19911031;第47卷(第5期);第766-771部分，尤其第766页第2.1节 * |
| 木瓜蛋白酶简介;沈家柏;《亚热带植物通讯》;19841231(第2期);第50-56页，尤其第55页流程图部分 * |
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