CN104255260A - Cultivation method of high-yield heat-resisting fritillaria thunbergii germplasms - Google Patents
Cultivation method of high-yield heat-resisting fritillaria thunbergii germplasms Download PDFInfo
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- CN104255260A CN104255260A CN201410507540.9A CN201410507540A CN104255260A CN 104255260 A CN104255260 A CN 104255260A CN 201410507540 A CN201410507540 A CN 201410507540A CN 104255260 A CN104255260 A CN 104255260A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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Abstract
The invention relates to a cultivation method of high-yield heat-resisting fritillaria thunbergii germplasms. The cultivation method is characterized by comprising the steps of a) selecting the fritillaria thunbergii bulbs just beginning dormant and controlling each bulb in the range of 22-33g, b) performing irradiation treatment by use of 137Cs by the dosage of 10-20Gy per 180-220 bulbs and at the dosage rate of 2Gy/Min, and c) planting the fritillaria thunbergii bulbs previously treated by irradiation by use of a conventional method and screening out the high-yield heat-resisting fritillaria thunbergii by the sprout tumble stage and the yield of the bulbs as assessment indexes. Compared with a traditional hybridization technique, the radiation induced mutation technique adopted in the cultivation method of the high-yield heat-resisting fritillaria thunbergii germplasm is capable of shortening the breeding period of new species of the fritillaria thunbergii, and the obtained new germplasms of the fritillaria thunbergii are capable of effectively delaying the sprout tumble stage, and are far higher than conventional species in propagation coefficient and absolute yield, and the difference is remarkable.
Description
Technical field
The present invention relates to new germ plasm and cultivate field, specifically refer to a kind of breeding method of medicinal plant fritillaria thunbergii high yield heat resistant type new germ plasm.
Background technology
Fritillaria thunbergii ((Fritillaria thunbergii Mi), another name resembles shellfish, pearl shellfish, belongs to Liliaceae (Liliaceae)).Fritillaria herbaceos perennial.Main component has zhebeiresinol, verticinone, Peimisine, the bulb of fritillary is fragrant, the bulb of fritillary is fixed, fritillaria thunbergii two etc., is used as medicine with dry bulb.Fritillaria thunbergii, as rare traditional Chinese medicine, has very high medical value.Record according to Compendium of Material Medica, bulb of fritillary cold nature, sweet-bitter flavor, cure mainly typhoid fever dysphoria with smothery sensation, cough only gas, five viscera settling, sharp marrow, dissolving phlegm, profit cardiopulmonary, have anti-inflammatory detumescence concurrently, the effects such as treatment carbuncle furuncle poison.Record according to " Chinese medicinal herbal " again: the bulb of fritillary has clearing heat and moistening lung, preventing phlegm from forming and stopping coughing, dissipating bind function., cold disease, sore swollen toxin sticky for phlegm-heat cough, phlegm uncomfortable in chest.
Fritillaria thunbergii proceeds to family's kind in clear Kangxu's age by wild, at that time by Xiangshan peasant planting out, therefore has again the title of " resembling shellfish ".By Xiangshan, import Yinxian County plantation before about more than 300 years into, Yinxian County becomes the main product ground of fritillaria thunbergii gradually.Fritillaria thunbergii reasonable time in planting range narrow and small, almost define " one ground produce, supply the whole nation " situation, resource is extremely in short supply.After six the seventies, under help from government, the positive introducing and planting in area that natural conditions are suitable, to the eighties in earlier stage, fritillaria thunbergii planting area expands rapidly.Especially after entering the nineties, along with going deep into of China's structural readjustment of rural industry, fritillaria thunbergii also to all round area expansion, progressively defines Nantong, Hangzhou, Yuyao, Zhejiang, Zhoushan, Jinhua, Deng Xin producing region, Lishui.
But because fritillaria thunbergii kind compares less, main based on narrow leaf kind, wide leaf kind (another name is for beautiful shellfish) and seediness kind in current production, find in production process in recent years, deterioration of variety phenomenon is more serious, main manifestations is that growth potential and marketing quality are unstable, degradation feature under resistance against diseases, has had a strong impact on the sound development of fritillaria thunbergii industry.Carrying out the research of fritillaria thunbergii breeding of new variety, is that current fritillaria thunbergii industry development needs one of bottleneck solved.
Summary of the invention
Technical problem to be solved by this invention is the breeding method providing a kind of high yield heat resistant type fritillaria thunbergii kind matter for the situation of prior art.
The present invention solves the problems of the technologies described above adopted technical scheme: the breeding method of this high yield heat resistant type fritillaria thunbergii kind matter, is characterized in that comprising the steps:
A) choose the Bulbs of Fritillaria thunbergii Miq just having entered resting stage, squama footpath size controls at 22-33g/;
B) carry out radiation treatment with 137Cs, the dosage in every 180-220 squama footpath is 10-20Gy, dose rate is 2Gy/Min;
C) female for the Zhejiang after radiation treatment shellfish bulb is planted according to a conventional method, to screen for performance assessment criteria obtain high yield heat resistant type fritillaria thunbergii to fall seedling stage and bulb yield.
Compared with conventional hybridization technology, radioinduction technology provided by the present invention can not only accelerate the seed selection cycle of fritillaria thunbergii new varieties, and the fritillaria thunbergii new germ plasm obtained, and it effectively can postpone down seedling stage, reproduction coefficient and absolute yield are far above conventional variety, and difference reaches significance level.
Accompanying drawing explanation
Fig. 1 is the embodiment of the present invention fritillaria thunbergii plant of falling seedling stage photo;
Fig. 2 is the fritillaria thunbergii plant of falling seedling stage photo in prior art;
Fig. 3 is the DNA bar code Molecular Identification result of ITS1 in the embodiment of the present invention;
Fig. 4 is the DNA bar code Molecular Identification result of ITS2 in the embodiment of the present invention.
Embodiment
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
Within 2010, choosing the weight that is in hibernation is that the Bulbs of Fritillaria thunbergii Miq of 22-33g/ is as sample.The resting stage of fritillaria thunbergii is the 5-9 month, selects the Bulbs of Fritillaria thunbergii Miq of resting stage in June as kind of a shellfish in the present embodiment.
In test, we find, the bulb size of planting shellfish is not the bigger the better, and the reproduction coefficient of planting shellfish bulb little is on the contrary high.But consider the using value of commodity shellfish, we select weight to be the medium kind of shellfish of 22-33g/.
Embodiment 1 to embodiment 6
2010 is one group with 200, and according to dosage rate is that 2Gy/Min irradiates, and radiation dose is respectively 0,10,20,30,40 and 60Gy, the bulb (M after radiation
0generation) by fritillaria thunbergii general planting method often row 12, spacing 5cm between, line space 15-20cm kind is planted in proving ground, Zhang Shui town, Yinzhou District, fritillaria thunbergii main product ground, and statistics emergence rate, Sheng phase plant plant height and absolute yield, result is as shown in table 1.
Table 1 M
0for the M obtained after Bulbs of Fritillaria thunbergii Miq plantation
1the field the Characters in generation
As can be seen from Table 1, different radiation dose can cause adverse influence to the emergence rate of Bulbs of Fritillaria thunbergii Miq, Growth and yield (bulb size and reproduction coefficient etc.).According to the general performance of Irradiated Progenies, 20Gy is selected to be treated to suitable.
The selection systems of new germ plasm: M
1plant 4 years continuously again in field for after bulb plantation results, obtain M
4generation.Observe the upgrowth situation of irradiation treatment plant, screen heat resistant type and high yield type new germ plasm, simultaneously at M to fall seedling stage and bulb yield for performance assessment criteria
4in generation, adopts DNA bar code ITS1 and ITS2 technology to carry out Molecular Identification.
Observe, at the M of 20Gy dose through the fritillaria thunbergii phenophase
4filter out the fritillaria thunbergii new germ plasm of the prolongation of falling seedling stage in offspring, result as shown in Figure 1.Fig. 2 is the existing commercially available conventional variety (i.e. embodiment 1) without radiation treatment.As seen from Figure 1, the withered and yellow degree of the fritillaria thunbergii plant of 20Gy process is starkly lower than the withered and yellow degree of conventional variety in Fig. 2.
Plant is early withered and yellow, shows that the breeding time of fritillaria thunbergii is shorter, is more unfavorable for metabolism and the accumulation of Bulbs of Fritillaria thunbergii Miq effective component.Can obviously be found out by Fig. 1 and Fig. 2, through the M of 20Gy dose
4the growth time of offspring fritillaria thunbergii overground part extends and greatly extends, for fritillaria thunbergii high yield has established Metabolic Basis;
Adopt traditional Chinese medicine material to survey product and investigate method to M with Other Main Agronomic Characters
4offspring fritillaria thunbergii offspring carries out output test, and adopt supercritical ultrasonics technology and HPLC method abstraction and quantification thunberg fritillary bulb omni-alkaloids content, result is as shown in table 2 simultaneously.
As shown in Table 2, this heat resistant type kind matter is compared with conventional variety (embodiment 1), and plant height and alkaloid are more or less the same, but reproduction coefficient and absolute yield are higher than conventional variety, improve 16.67% and 7.95% respectively, difference reaches significance level.Show thus, heat resistant type new germ plasm of the present invention is because being delayed down seedling stage and then improve reproduction coefficient and the absolute yield of fritillaria thunbergii.
The output of table 2 heat resistant type fritillaria thunbergii new germ plasm and effective component situation
Finally, we to the heat resistant type fritillaria thunbergii kind matter of genetic stability respectively with ITS1 and ITS2 for primer has carried out the Molecular Identification of DNA bar code technology, result is as shown in Figure 3 and Figure 4; Check order to electrophoresis product, result is as shown in table 3 simultaneously.Being found by sequencing result, there is number of base sudden change in the DNA of heat resistant type fritillaria thunbergii new germ plasm.Show thus, the heat resistant type fritillaria thunbergii that technical scheme of the present invention obtains is new germ plasm.
Table 3 ITS1, ITS2 sequence result fractional mutations base information
Claims (1)
1. a breeding method for high yield heat resistant type fritillaria thunbergii kind matter, is characterized in that comprising the steps:
A) choose the Bulbs of Fritillaria thunbergii Miq just having entered resting stage, squama footpath size controls at 22-33g/;
B) carry out radiation treatment with 137Cs, the dosage in every 180-220 squama footpath is 10-20Gy, dose rate is 2Gy/Min;
C) female for the Zhejiang after radiation treatment shellfish bulb is planted according to a conventional method, to screen for performance assessment criteria obtain high yield heat resistant type fritillaria thunbergii to fall seedling stage and bulb yield.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0597650A (en) * | 1991-09-03 | 1993-04-20 | Tsuneo Nanba | Whitening cosmetic |
| JPH0616530A (en) * | 1992-07-03 | 1994-01-25 | Mikimoto Pharmaceut Co Ltd | Cosmetic |
| CN1103542A (en) * | 1993-12-09 | 1995-06-14 | 王余军 | Breeding method of high-yield variety of cotton with several bolls in one node |
| CN101185419A (en) * | 2007-12-12 | 2008-05-28 | 新疆农业科学院园艺作物研究所 | Local flavor sour-sweet melon series hybrid breeding method |
| CN101213937A (en) * | 2007-12-28 | 2008-07-09 | 浙江省农业科学院 | Method for cultivating detoxification tissue culture bulb of fritillaria thunbergii |
-
2014
- 2014-09-28 CN CN201410507540.9A patent/CN104255260A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0597650A (en) * | 1991-09-03 | 1993-04-20 | Tsuneo Nanba | Whitening cosmetic |
| JPH0616530A (en) * | 1992-07-03 | 1994-01-25 | Mikimoto Pharmaceut Co Ltd | Cosmetic |
| CN1103542A (en) * | 1993-12-09 | 1995-06-14 | 王余军 | Breeding method of high-yield variety of cotton with several bolls in one node |
| CN101185419A (en) * | 2007-12-12 | 2008-05-28 | 新疆农业科学院园艺作物研究所 | Local flavor sour-sweet melon series hybrid breeding method |
| CN101213937A (en) * | 2007-12-28 | 2008-07-09 | 浙江省农业科学院 | Method for cultivating detoxification tissue culture bulb of fritillaria thunbergii |
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