CN104237540B - A kind of tube-type chemical electrochemiluminescent immunoassay checkout gear - Google Patents
A kind of tube-type chemical electrochemiluminescent immunoassay checkout gear Download PDFInfo
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- CN104237540B CN104237540B CN201410432647.1A CN201410432647A CN104237540B CN 104237540 B CN104237540 B CN 104237540B CN 201410432647 A CN201410432647 A CN 201410432647A CN 104237540 B CN104237540 B CN 104237540B
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- liquid feeding
- fixedly connected
- guide rail
- tube
- checkout gear
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- 238000003018 immunoassay Methods 0.000 title claims abstract description 30
- 239000000126 substance Substances 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 51
- 238000001514 detection method Methods 0.000 claims abstract description 15
- 238000000429 assembly Methods 0.000 claims description 16
- 230000000712 assembly Effects 0.000 claims description 16
- 238000007664 blowing Methods 0.000 claims description 11
- 239000000523 sample Substances 0.000 claims description 9
- 238000010276 construction Methods 0.000 claims description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000005622 photoelectricity Effects 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 claims 1
- 238000005516 engineering process Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004035 construction material Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Abstract
A kind of tube-type chemical electrochemiluminescent immunoassay checkout gear, comprising: lifter, liquid feeding structure, detection architecture, movable block, moving guide rail, motor, pump combination; Described lifter is fixedly connected with liquid feeding structure, and described detection architecture is positioned at the side of movable block, and described moving guide rail is positioned at the below of movable block, and described pump combination is connected with liquid feeding structure by flexible pipe. The invention provides a kind of volume little, simple in structure, convenient operation, with low cost, control precisely and energy and the matching used chemiluminescence immunoassay checkout gear of other chemiluminescence immunoassay detection parts.
Description
Technical field
The present invention relates to a kind of new type medical equipment, relate in particular to a kind of tube-type chemical electrochemiluminescent immunoassay checkout equipment.
Background technology
Chemiluminescence immunoassay technology, because its degree of accuracy is high, easy to operate, is widely applied in field of biological detection.Microwell plate Reusability in traditional board-like chemiluminescence immunoassay technology, exists and pollutes; Board-like is generally wide variety of glow-type, lightSub-collection efficiency is low. Existing Full-automatic chemiluminescence immune detection instrument is generally bulky, is not suitable for hospital or laboratory etc.Small sample amount place is used. After prior art magnetic separation, sample distribution can be inhomogeneous, and the magnetic-particle after separation flocks togetherBe not easy to disperse, use chemiluminescence immunoassay technology, cause follow-up detected value deviation.
Summary of the invention
Problem to be solved, the invention provides a kind of volume little, simple in structure, and convenient operation is with low cost, controls essenceStandard and energy and the matching used chemiluminescence immunoassay checkout gear of other chemiluminescence immunoassay detection parts.
Technical scheme: a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear, comprising: lifter, liquid feeding structure, detection architecture,Movable block, moving guide rail, motor, pump combination; Described lifter is fixedly connected with liquid feeding structure, and described detection architecture is positioned atThe side of movable block, described moving guide rail is positioned at the below of movable block, and described pump combines by flexible pipe and liquid feeding structure phaseConnect.
Further technical scheme, described lifter comprises lifter support, guide rail, guide rail slide block, take-up housing, transmissionStructure, driving block, described drive mechanism comprises wheel shaft, motor shaft, the first synchronizing wheel, the second synchronizing wheel, belt;
Wheel shaft and motor shaft are installed respectively in described lifter cantilever tip and bottom, and it is first synchronous that described wheel shaft connectsWheel, described motor shaft connects the second synchronizing wheel, and described belt sleeve is on the first synchronizing wheel and the second synchronizing wheel, and described leadsRail is fixedly connected on lifter support, and described guide rail slide block is placed on guide rail, and described take-up housing is fixed on belt alsoBe fixedly connected with guide rail slide block;
Described motor comprises the first motor, the second motor;
Described liquid feeding structure comprises: liquid feeding vertical rod, liquid feeding needle tubing seat, needle tubing lid, three needle assemblies, described liquid feeding vertical rodBe fixedly connected with driving block by iron stator, vertical rod is fixedly connected with liquid feeding in described liquid feeding needle tubing seat one end, anotherEnd is provided with three needle assemblies, and described needle tubing lid is placed in the top of liquid feeding needle tubing seat;
Described detection architecture is photomultiplier, and described movable block inside is provided with reaction cup groove, and described photoelectricity doublyIncreasing pipe is fixedly connected with by probe sealing gasket with movable block;
Described moving guide rail comprises movable base, base contiguous block, screw mandrel, slide block, track, described movable base positionIn the below of movable block and be fixedly connected with, described movable base is stuck on screw mandrel, and described base contiguous block is fixedly connected onMovable base both sides, described slide block is positioned at base contiguous block below and is fixedly connected with, and described slide block is placed on track.
Further technical scheme, three described needle assemblies comprise blowing needle, add excimer 1 pin and add excimer 2 pins, instituteThe gas hole of the blowing needle of stating is oblique.
Further technical scheme, one end of described liquid feeding needle tubing seat is provided with hickey and connects with needle tubing hermetically-sealed constructionConnect.
Further technical scheme, the material of described needle tubing hermetically-sealed construction is silica gel.
Further technical scheme, described photomultiplier is also provided with photomultiplier transit pipe holder, and and photomultiplierBe fixedly connected with.
Further technical scheme, described screw mandrel one end is also provided with electric machine support.
Further technical scheme, the first described motor is positioned at lifter cradle back and connects with lifter support is fixingConnect, the second motor is positioned at electric machine support lateral surface and is connected with screw mandrel.
Further technical scheme, described lifter is also provided with liquid feeding catch, and it is sliding that described liquid feeding catch is fixed on transmissionOn piece.
Further technical scheme, described pump combination comprises pump and pump bracket, and pump is fixedly connected with pump bracket, and described pump is logicalCrossing flexible pipe is connected with three needle assemblies.
Beneficial effect:
Technical scheme provided by the invention, can problem to be solved, the invention provides a kind of volume little, structure letterSingle, convenient operation, with low cost, control precisely and energy and the matching used chemiluminescence of other chemiluminescence immunoassay detection partsImmunoassay device. The reaction tube of tubular type is all disposable, be difficult for contaminated, and tubular type adopt technology be flash type,Photon collection efficiency is high.
1, three needle assemblies comprise blowing needle, add excimer 1 pin and add excimer 2 pins, and wherein blowing needle gas hole is oblique,In reaction cup liquid surface, constantly blow, and then drive liquid motion, make reactant dispersed, improve experiment preciselyDegree.
2, one end of three described needle assemblies is provided with hickey and is connected needle tubing hermetically-sealed construction pin with needle tubing hermetically-sealed constructionMaterial be silica gel, good airproof performance, retractility is good, corrosion-resistant, has stronger anti-microbial property, especially pin group parts is played to guarantorProtect effect, needle tubing hermetically-sealed construction can stop that light enters, and keeps darkroom environment, improves experimental precision, and to three needle assembliesPlay a protective role, prevent that the content of reaction cup is contaminated, affect experimental precision.
3, photomultiplier transit pipe holder fixed light electricity multiplier tube, makes to detect light and directly arrives detection window, reduces the wastage.
4, electric machine support plays the fixing effect of supporting, and motor and other mechanical parts are separated into relatively independent districtTerritory, in the time that certain parts of driven by motor machinery rotate, drops to very low level by vibrations. The position of the motor distance control part separately of arrangingPart is nearer, and convenient wiring and maintenance, save space, facilitates radiating management, reduces the wastage.
5, lifter is also provided with liquid feeding catch, prevents that lifter from exceeding predetermined movement scope, affects machine and uses.
Brief description of the drawings
The three-dimensional structure diagram of Fig. 1, a kind of tube-type chemical electrochemiluminescent immunoassay of the present invention checkout gear;
Fig. 2, a kind of tube-type chemical electrochemiluminescent immunoassay of the present invention checkout gear mid-riser structure chart;
The side view of Fig. 3, a kind of tube-type chemical electrochemiluminescent immunoassay of the present invention checkout gear;
Moving guide rail structure chart in Fig. 4, a kind of tube-type chemical electrochemiluminescent immunoassay of the present invention checkout gear;
The photomultiplier structure chart of Fig. 5, a kind of tube-type chemical electrochemiluminescent immunoassay of the present invention checkout gear;
The pump combination assumption diagram of Fig. 6, a kind of tube-type chemical electrochemiluminescent immunoassay of the present invention checkout gear.
Three needle assemblies structure charts of Fig. 7, a kind of tube-type chemical electrochemiluminescent immunoassay of the present invention checkout gear.
Specific embodiment
A kind of tube-type chemical electrochemiluminescent immunoassay checkout gear as shown in Figures 1 to 6, comprising: lifter 1, liquid feeding structure 2, inspectionGeodesic structure 3, movable block 4, moving guide rail 5, motor 6, pump combination 7; Described lifter 1 is fixedly connected with liquid feeding structure 2, described inDetection architecture 3 be positioned at the side of movable block 4, described moving guide rail 5 is positioned at the below of movable block 4, described pump combination 7 is logicalCrossing flexible pipe is connected with liquid feeding structure 2.
Described lifter 1 comprises lifter support 1-1, guide rail 1-2, guide rail slide block 1-3, take-up housing 1-4, drive mechanism1-5, driving block 1-6, described drive mechanism 1-5 comprises wheel shaft 1-5-1, motor shaft 1-5-2, the first synchronizing wheel 1-5-3, theTwo synchronizing wheel 1-5-4, belt 1-5-5;
Wheel shaft 1-5-1 and motor shaft 1-5-2, described wheel are installed respectively in described lifter support 1-1 top and bottomAxle 1-5-1 connects the first synchronizing wheel 1-5-3, and described motor shaft 1-5-2 connects the second synchronizing wheel 1-5-4, described belt 1-5-5 is enclosed within the first synchronizing wheel 1-5-3 and the second synchronizing wheel 1-5-4 is upper, and described guide rail 1-2 is fixedly connected on lifter support 1-On 1, it is upper that described guide rail slide block 1-3 is placed in guide rail 1-2, and it is upper and sliding with guide rail that described take-up housing 1-4 is fixed on belt 1-5-5Piece 1-3 is fixedly connected with;
Described motor 6 comprises the first motor 6-1, the second motor 6-2;
Described liquid feeding structure 2 comprises: liquid feeding vertical rod 2-1, and liquid feeding needle tubing seat 2-2, needle tubing lid 2-3, three needle assemblies 2-4,Described liquid feeding vertical rod 2-1 is fixedly connected with driving block 1-6 by iron stator, described liquid feeding needle tubing seat 2-2 one end2-1 is fixedly connected with liquid feeding vertical rod, and the other end is provided with three needle assemblies 2-4, and described needle tubing lid 2-3 is placed in liquid feeding needle tubing seat 2-2Top;
Described detection architecture 3 is photomultipliers, and described movable block 4 inside are provided with reaction cup groove 4-1, described lightElectricity multiplier tube is fixedly connected with by probe sealing gasket 3-1 with movable block 4;
Described moving guide rail 5 comprises movable base 5-1, base contiguous block 5-2, and screw mandrel 5-3, slide block 5-4, track 5-5,Described movable base 5-1 is positioned at the below of movable block 4 and is fixedly connected with, and it is upper that described movable base 5-1 is stuck in screw mandrel 5-3,Described base contiguous block 5-2 is fixedly connected on movable base 5-1 both sides, and described slide block 5-4 is positioned under base contiguous block 5-2Side and being fixedly connected with, described slide block 5-4 is placed on track 5-5.
Three described needle assemblies 2-4 comprise blowing needle 2-4-1, add excimer 1 pin 2-4-2 and add excimer 2 pin 2-4-3,The gas hole of described blowing needle 2-4-1 is oblique.
One end of described liquid feeding needle tubing seat 2-2 is provided with hickey and is connected with needle tubing hermetically-sealed construction 2-5.
The material of described needle tubing hermetically-sealed construction 2-5 is silica gel.
Described photomultiplier is also provided with photomultiplier transit pipe holder 8, and is fixedly connected with photomultiplier.
Described screw mandrel 5-3 one end is also provided with electric machine support 9.
The first described motor 6-1 is positioned at the lifter support 1-1 back side and is fixedly connected with second with lifter support 1-1Motor 6-2 is positioned at electric machine support 9 lateral surfaces and is connected with screw mandrel 5-3.
Described lifter 1 is also provided with liquid feeding catch 1-7, and described liquid feeding catch 1-7 is fixed on driving block 1-6.
Described pump combination 7 comprises pump 7-1 and pump bracket 7-2, and pump 7-1 is fixedly connected with pump bracket 7-2, and described pump 7-1 is logicalCrossing flexible pipe is connected with three needle assemblies 2-4.
The course of work:
Reaction cup is put into movable block 4 reaction cup groove 4-1, and the second motor 6-2 drives movable block 4
Move and reach detection position along moving guide rail 5, the first motor 6-1 drives drive mechanism 1-5 in lifter 1, and thenDrive liquid feeding vertical rod 2-1 to move downward, in the time that liquid feeding catch 1-7 reaches liquid feeding desired location, sensed drive mechanism by optocoupler1-5 stop motion. The liquid feeding needle tubing seat 2-2 that liquid feeding vertical rod 2-1 is fixedly connected with is connected with three needle assemblies 2-4, three needle assemblies 2-4Enter in reaction cup, needle tubing hermetically-sealed construction 2-5 covers movable block 4 reaction cup groove 4-1 notch places, obtains sealing effectiveness.
Add excimer 1 pin 2-4-2 and add excimer 1, blowing needle 2-4-1 starts to blow and mixes, and adds excimer 2 pin 2-4-3 add excimer 2 again, and photomultiplier starts to absorb after photon calculates number of photons and detects, and liquid feeding structure 2, lifter 1 are multiplePosition, movable block 4 resets.
Photomultiplier is (CH297-01) (Beijing Bin Song photoelectric technology limited company)
For same sample (the PCT sample of 1.0ng/ml), carry out 10 groups of measurements, measure 10 times for every group. Draw data asUnder:
| The technical program application of sample (not blowing) coefficient of variation CV | The technical program application of sample (air blowing) coefficient of variation CV | |
| 1、 | 6.8% | 3.1% |
| 2、 | 11.2% | 2.0% |
| 3、 | 7.2% | 2.7% |
| 4 | 8.9% | 4.1% |
| 5 | 8.0% | 0.9% |
| 6 | 14.6% | 2.9% |
| 7 | 18.6% | 3.7% |
| 8 | 5.7% | 3.2% |
| 9 | 12.1% | 1.8% |
| 10 | 8.2% | 3.2% |
| Conclusion: | Be less than 19% | Be less than 5% |
Claims (9)
1. a tube-type chemical electrochemiluminescent immunoassay checkout gear, is characterized in that, comprising: lifter (1), liquid feeding structure (2), detectsStructure (3), movable block (4), moving guide rail (5), motor (6), pump combination (7); Described lifter (1) and liquid feeding structure (2)Be fixedly connected with, described detection architecture (3) is positioned at the side of movable block (4), and described moving guide rail (5) is positioned at movable block (4)Below, described pump combination (7) is connected with liquid feeding structure (2) by flexible pipe;
Described lifter (1) comprises lifter support (1-1), guide rail (1-2), and guide rail slide block (1-3), take-up housing (1-4), passesMoving structure (1-5), transmission is slided (1-6), and described drive mechanism (1-5) comprises wheel shaft (1-5-1), motor shaft (1-5-2), theOne synchronizing wheel (1-5-3), the second synchronizing wheel (1-5-4), belt (1-5-5);
Wheel shaft (1-5-1) and motor shaft (1-5-2) are installed respectively in described lifter support (1-1) top and bottom, describedWheel shaft (1-5-1) connects the first synchronizing wheel (1-5-3), and described motor shaft (1-5-2) connects the second synchronizing wheel (1-5-4), instituteThe belt (1-5-5) of stating is enclosed within the first synchronizing wheel (1-5-3) and the second synchronizing wheel (1-5-4) is upper, and described guide rail (1-2) is fixingBe connected to lifter support (1-1) upper, it is upper that described guide rail slide block (1-3) is placed in guide rail (1-2), described take-up housing (1-4)Be fixed on belt (1-5-5) and be fixedly connected with guide rail slide block (1-3);
Described motor (6) comprises the first motor (6-1), the second motor (6-2);
Described liquid feeding structure (2) comprising: liquid feeding vertical rod (2-1), liquid feeding needle tubing seat (2-2), needle tubing lid (2-3), three needle assemblies(2-4), described liquid feeding vertical rod (2-1) is fixedly connected with driving block (1-6) by iron stator, described liquid feeding needle tubingSeat (2-2) one end is fixedly connected with liquid feeding vertical rod (2-1), and the other end is provided with three needle assemblies (2-4), described needle tubing lid (2-3)Be placed in the top of liquid feeding needle tubing seat (2-2);
Described detection architecture (3) is photomultiplier, and described movable block (4) inside is provided with reaction cup groove (4-1), describedPhotomultiplier is fixedly connected with by probe sealing gasket (3-1) with movable block (4);
Described moving guide rail (5) comprises movable base (5-1), base contiguous block (5-2), screw mandrel (5-3), slide block (5-4), railRoad (5-5), described movable base (5-1) is positioned at the below of movable block (4) and is fixedly connected with, described movable base (5-1)Be stuck in screw mandrel (5-3) upper, described base contiguous block (5-2) is fixedly connected on movable base (5-1) both sides, described slide block(5-4) be positioned at base contiguous block (5-2) below and be fixedly connected with, described slide block (5-4) is placed on track (5-5).
2. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 1, is characterized in that, three described pin groupsPart (2-4) comprises blowing needle (2-4-1), adds excimer 1 pin (2-4-2) and adds excimer 2 pins (2-4-3), described blowing needle(2-4-1) gas hole is oblique.
3. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 1, is characterized in that described liquid feeding pinOne end of base (2-2) is provided with hickey and is connected with needle tubing hermetically-sealed construction (2-5).
4. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 3, is characterized in that, described needle tubing is closeThe material of seal structure (2-5) is silica gel.
5. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 1, is characterized in that, described photoelectricity doublyIncrease pipe and be also provided with photomultiplier transit pipe holder (8), and be fixedly connected with photomultiplier.
6. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 1, is characterized in that described screw mandrel(5-3) one end is also provided with electric machine support (9).
7. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 1, is characterized in that the first described electricityMachine (6-1) is positioned at lifter support (1-1) back side and is fixedly connected with lifter support (1-1), and the second motor (6-2) is positioned at electricityMachine support (9) lateral surface and being connected with screw mandrel (5-3).
8. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 1, is characterized in that described lifter(1) be also provided with liquid feeding catch (1-7), described liquid feeding catch (1-7) is fixed on driving block (1-6).
9. a kind of tube-type chemical electrochemiluminescent immunoassay checkout gear according to claim 1, is characterized in that, described pump combination(7) comprise pump (7-1) and pump bracket (7-2), pump (7-1) is fixedly connected with pump bracket (7-2), described pump (7-1) by flexible pipe withThree needle assemblies (2-4) connect.
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| CN104237540B true CN104237540B (en) | 2016-05-04 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105628687B (en) * | 2016-02-24 | 2018-08-07 | 南京诺尔曼生物技术有限公司 | A kind of Full-automatic chemiluminescence analyzer |
| CN108362642B (en) * | 2018-02-09 | 2023-11-17 | 深圳市活水床旁诊断仪器有限公司 | Chemiluminescence immunoassay photometry device and method |
| CN109374910A (en) * | 2018-10-16 | 2019-02-22 | 基蛋生物科技股份有限公司 | Cleaning arm and analysis and test device |
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| CN101769858A (en) * | 2010-02-05 | 2010-07-07 | 深圳市新产业生物医学工程有限公司 | Automatic detection device and detection method thereof |
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| CN204065108U (en) * | 2014-08-29 | 2014-12-31 | 南京诺尔曼生物技术有限公司 | A kind of tube-type chemical electrochemiluminescent immunoassay pick-up unit |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3307113B2 (en) * | 1994-09-22 | 2002-07-24 | スズキ株式会社 | Enzyme immunoassay analyzer |
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| EP0905514A1 (en) * | 1997-09-27 | 1999-03-31 | Horiba, Ltd. | Blood cell count/immunoassay apparatus using whole blood |
| CN101769858A (en) * | 2010-02-05 | 2010-07-07 | 深圳市新产业生物医学工程有限公司 | Automatic detection device and detection method thereof |
| CN103399161A (en) * | 2013-07-17 | 2013-11-20 | 深圳市新产业生物医学工程股份有限公司 | Portable full-automatic chemiluminescence immune assay system and assay method thereof |
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