CN104230672A - Method for extracting piceatannol from fresh passion fruit seeds - Google Patents
Method for extracting piceatannol from fresh passion fruit seeds Download PDFInfo
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- CN104230672A CN104230672A CN201410450742.4A CN201410450742A CN104230672A CN 104230672 A CN104230672 A CN 104230672A CN 201410450742 A CN201410450742 A CN 201410450742A CN 104230672 A CN104230672 A CN 104230672A
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- China
- Prior art keywords
- piceatannol
- seed
- herba passiflorae
- fresh herba
- passiflorae caeruleae
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/004—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring by obtaining phenols from plant material or from animal material
Abstract
The invention discloses a method for preparing piceatannol from fresh passion fruit seeds. The method comprises the following steps: 1) taking the fresh passion fruit seeds, crushing, adding a saturated lime solution for soaking and extraction, regulating the pH of an extract solution to 8-10, performing interception and separation with an ultra-filtration membrane, collecting permeate, concentrating the permeate with a nano-filtration membrane with the interception molecular weight of 100-200, regulating the pH of concentrate to 3-5.5, adding a biological enzyme, performing heat preservation and enzymolysis, and performing decompression concentration and precipitation to obtain a crude extract; and 2) dissolving the crude extract with ethanol, adding an aluminum oxide column to adsorb impurities, thus obtaining a solution below the column, adding water, standing, crystallizing and drying to obtain the product. By adopting the method to produce piceatannol, the process is simple and easy to operate, the energy consumption is low and industrial production is easily realized by the method.
Description
Technical field
The invention belongs to biological technical field, particularly relate to one and prepare piceatannol method from fresh Herba Passiflorae Caeruleae seed.
Background technology
Piceatannol, CAS accession number 10083-24-6, molecular formula C14H12O4, molecular weight 244.24, molecular structure:
Piceatannol has superpower antioxygenation, suppresses non-receptor activation enzyme, spleen butyric acid kinases and Lymphocyte-specific protein matter Tyrosylprotein kinase.Herba Passiflorae Caeruleae is Passifloraceae, and fruit is rich in 17 seed amino acids of needed by human body, multivitamin and carotenoid and various trace element, and soluble solid 15-16%, total acid content 3.8-4%, sweet acid is moderate.Normal edible Purple Granadilla and processed goods thereof, to human body have aid digestion, can reduce phlegm, control deficiency of the kidney, produce refreshing effect to the mind, invigorate blood circulation keep fit, sedation-analgesia, the effect such as decompression lipopenicillinase.Seed Herba Passiflorae Caeruleae seed weight account for whole fruit heavy 8% ~ 15%, and containing rich in protein and fat in seed, also containing a kind of antioxidant piceatannol.
Pass through searching document, the document preparing piceatannol at present from plant discloses less, and the method disclosed as patent " containing piceatannol composition and the preparation method containing piceatannol composition " is that organic reagent extraction is drying to obtain, and piceatannol purity is lower, extraction efficiency is low, seriously polluted.
Summary of the invention
The present invention will address the deficiencies of the prior art and deficiency, provides a kind of and prepare piceatannol method from fresh Herba Passiflorae Caeruleae seed.
The present invention realizes by the following technical solutions:
One prepares piceatannol method from fresh Herba Passiflorae Caeruleae seed, it is characterized in that comprising following steps:
1) get fresh Herba Passiflorae Caeruleae seed feed to pulverize, add saturated limewater solution soaking to extract, extracting solution regulates pH to be 8-10, retains separation by ultra-filtration membrane, collects permeate, permeate again by molecular weight cut-off be 100-200 nanofiltration membrane concentrate, concentrated solution regulates PH to 3-5.5, adds biological enzyme, insulation enzymolysis, concentrating under reduced pressure precipitates, and obtains crude extract;
2) by above-mentioned crude extract dissolve with ethanol, add alumina column adsorbing contaminant, obtain lower column liquid, add water placement crystallization, dry, to obtain final product.
Soak extraction condition described in step 1) is: to add saturated limewater amount be consumption be raw material weight 4-6 doubly, soaking temperature 40-60 DEG C, soak time 1-2 hour, soak extraction number of times is 2-3 time.
Described in step 1), membrane sepn condition is: ultra-filtration membrane is the hollow cellulose film of molecular weight cut-off 1000-3000.
The optional cellulase of enzyme described in step 1), polygalacturonase and amylase.
Adopt the present invention to produce piceatannol, energy consumption is low, and technological operation is simple, and products obtained therefrom purity is high, is easy to suitability for industrialized production.
Further illustrate the present invention below in conjunction with embodiment, but the scope of protection of present invention is not limited to the following example.
Embodiment
Embodiment 1:
Get fresh Herba Passiflorae Caeruleae seed to pulverize, get 10kg, add in 40L saturated limewater solution, heat to 40 DEG C, be incubated 1 hour, filter extracting solution, repeat extraction 3 times, united extraction liquid hydrochloric acid is adjusted to pH8, it is ultrafiltration in the hollow cellulose ultra-filtration membrane of 3000 that filtration adds molecular weight cut-off, collect permeate, permeate again by molecular weight cut-off be 200 nanofiltration membrane concentrate, collect concentrated solution, PH to 4 is regulated with hydrochloric acid, heat to 50 DEG C, add 0.1% cellulase of amount of liquid, insulation enzymolysis 3 hours, concentrated enzymolysis solution, filter after precipitation, crude extract with 95% the saturated dissolving of alcohol reflux, place room temperature and add neutral alumina column adsorbing contaminant, collect lower column liquid, add isopyknic water, place crystallization, filtering crystals, cryodrying obtains 8.9g, content 98.3% after testing.
Embodiment 2:
Get fresh Herba Passiflorae Caeruleae seed to pulverize, get 10kg, add in 60L saturated limewater solution, heat to 50 DEG C, be incubated 2 hours, filter extracting solution, repeat extraction 3 times, united extraction liquid hydrochloric acid is adjusted to pH9, it is ultrafiltration in the hollow cellulose ultra-filtration membrane of 1000 that filtration adds molecular weight cut-off, collect permeate, permeate again by molecular weight cut-off be 100 nanofiltration membrane concentrate, collect concentrated solution, PH to 3 is regulated with hydrochloric acid, heat to 55 DEG C, add 0.3% amylase of amount of liquid, insulation enzymolysis 2 hours, concentrated enzymolysis solution, filter after precipitation, crude extract with 90% the saturated dissolving of alcohol reflux, place room temperature and add neutral alumina column adsorbing contaminant, collect lower column liquid, add isopyknic water, place crystallization, filtering crystals, cryodrying obtains 10.2g, content 96.1% after testing.
Embodiment 3:
Get fresh Herba Passiflorae Caeruleae seed to pulverize, get 10kg, add in 50L saturated limewater solution, heat to 60 DEG C, be incubated 1 hour, filter extracting solution, repeat extraction 3 times, united extraction liquid hydrochloric acid is adjusted to pH10, it is ultrafiltration in the hollow cellulose ultra-filtration membrane of 2000 that filtration adds molecular weight cut-off, collect permeate, permeate again by molecular weight cut-off be 200 nanofiltration membrane concentrate, collect concentrated solution, PH to 5.5 is regulated with hydrochloric acid, heat to 60 DEG C, add 0.2% amylase of amount of liquid, insulation enzymolysis 4 hours, concentrated enzymolysis solution, filter after precipitation, crude extract with 99% the saturated dissolving of alcohol reflux, place room temperature and add neutral alumina column adsorbing contaminant, collect lower column liquid, add isopyknic water, place crystallization, filtering crystals, cryodrying obtains 9.5g, content 95.2% after testing.
Claims (4)
1. from fresh Herba Passiflorae Caeruleae seed, prepare a piceatannol method, it is characterized in that comprising following steps:
1) get fresh Herba Passiflorae Caeruleae seed to pulverize, add saturated limewater solution soaking to extract, extracting solution regulates pH to be 8-10, retains separation by ultra-filtration membrane, collects permeate, permeate again by molecular weight cut-off be 100-200 nanofiltration membrane concentrate, concentrated solution regulates PH to 3-5.5, adds biological enzyme, insulation enzymolysis, concentrating under reduced pressure precipitates, and obtains crude extract;
2) by above-mentioned crude extract dissolve with ethanol, add alumina column adsorbing contaminant, obtain lower column liquid, add water placement crystallization, dry, to obtain final product.
2. from fresh Herba Passiflorae Caeruleae seed, prepare piceatannol method as claimed in claim 1, the soak extraction condition described in step 1) that it is characterized in that is: to add saturated limewater amount be consumption be raw material weight 4-6 doubly, soaking temperature 40-60 DEG C, soak time 1-2 hour, soak extraction number of times is 2-3 time.
3. from fresh Herba Passiflorae Caeruleae seed, prepare piceatannol method as claimed in claim 1, it is characterized in that described in step 1), membrane sepn condition is: ultra-filtration membrane is the hollow cellulose film of molecular weight cut-off 1000-3000.
4. from fresh Herba Passiflorae Caeruleae seed, prepare piceatannol method as claimed in claim 1, it is characterized in that the optional cellulase of enzyme described in step 1), polygalacturonase and amylase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410450742.4A CN104230672A (en) | 2014-09-05 | 2014-09-05 | Method for extracting piceatannol from fresh passion fruit seeds |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410450742.4A CN104230672A (en) | 2014-09-05 | 2014-09-05 | Method for extracting piceatannol from fresh passion fruit seeds |
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| CN104230672A true CN104230672A (en) | 2014-12-24 |
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| CN201410450742.4A Pending CN104230672A (en) | 2014-09-05 | 2014-09-05 | Method for extracting piceatannol from fresh passion fruit seeds |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108697610A (en) * | 2015-12-21 | 2018-10-23 | 科学发展实验室 | Passionflower seed extract and cosmetics, drug or dermatological compositions comprising it |
| CN110655452A (en) * | 2019-09-29 | 2020-01-07 | 钟华 | Method for extracting piceatannol from mixture of passion fruit, grape and blueberry and application of piceatannol |
| CN111704530A (en) * | 2020-07-07 | 2020-09-25 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rheum officinale, microcapsule molecule embedding method and application of piceatannol in cosmetics |
| CN111763141A (en) * | 2020-07-07 | 2020-10-13 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rhubarb medicinal materials, microcapsule molecule embedding method and application of microcapsule molecule embedding method in food and health care products |
-
2014
- 2014-09-05 CN CN201410450742.4A patent/CN104230672A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108697610A (en) * | 2015-12-21 | 2018-10-23 | 科学发展实验室 | Passionflower seed extract and cosmetics, drug or dermatological compositions comprising it |
| CN110655452A (en) * | 2019-09-29 | 2020-01-07 | 钟华 | Method for extracting piceatannol from mixture of passion fruit, grape and blueberry and application of piceatannol |
| CN111704530A (en) * | 2020-07-07 | 2020-09-25 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rheum officinale, microcapsule molecule embedding method and application of piceatannol in cosmetics |
| CN111763141A (en) * | 2020-07-07 | 2020-10-13 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rhubarb medicinal materials, microcapsule molecule embedding method and application of microcapsule molecule embedding method in food and health care products |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141224 |
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| WD01 | Invention patent application deemed withdrawn after publication |