CN104226389A - Wax screen printing processing method for cloth-based microfluidic analytical chip - Google Patents
Wax screen printing processing method for cloth-based microfluidic analytical chip Download PDFInfo
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- CN104226389A CN104226389A CN201410487022.5A CN201410487022A CN104226389A CN 104226389 A CN104226389 A CN 104226389A CN 201410487022 A CN201410487022 A CN 201410487022A CN 104226389 A CN104226389 A CN 104226389A
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Abstract
The invention discloses a wax screen printing processing method for a cloth-based microfluidic analytical chip. The method comprises the following steps: placing cotton cloth in a container, adding water to the container and boiling; taking out the cotton cloth, drying the moisture, placing the dried cotton cloth in another container, and adding a sodium carbonate solution to soak the cotton cloth; taking out the cotton cloth, washing the cloth with water and drying; flattening the processed cotton cloth on a platform, covering a screen printing board with a designed pattern on the cotton cloth, waxing and milling; roasting the screen board and the cotton cloth together; after roasting, separating the cotton cloth from the screen board to obtain the cloth-based microfluidic analytical chip. The method disclosed by the invention is to directly coat the wax on the cotton cloth through the screen board, and then heat and fuse the wax to penetrate in the cotton cloth without other media to transfer, so that the utilization efficiency of the wax is high and the utilization effect is good. In addition, the chip prepared by the method has the characteristics of high flux and high resolution.
Description
Technical field
The invention belongs to micro-fluidic chip processing technique field, be specifically related to a kind of wax wire mark processing method of fabricbase microfluidic analysis chip.
Background technology
In recent years, the development of microfluidic analysis chip technology is very fast, be applied to chemical association area at first, be introduced into afterwards and be applied to biology, medical domain, just cross over the multi-disciplinary frontier such as chemistry, biology, medical science, physics as one now, have good development prospect, the development for the medical detection technique of entire society has huge meaning.What is more important, in order to improve the medical technique level of resource poor, economic Backward area, the microfluidic analysis chip technology cheap in the urgent need to people's research cost, light weight is easy, simple to operate, stability is strong, sense cycle is short, highly sensitive.At present, the researcher in numerous micro-fluidic field is just for this target and unremitting effort.
Now, usually the macromolecule polymeric material such as the inorganic material such as glass, silicon chip, quartz and PDMS (dimethyl silicone polymer), PMMA (polymethyl methacrylate), PC (Merlon) for making the backing material of microfluidic analysis chip, its chip technology of preparing is quite ripe, such as ultraviolet photolithographic, dry/wet etching erosion, Soft lithograph etc.But, glass, silicon chip and quartz substrate material and related processing equipment price relatively high; High molecular polymer often needs to use extra curing agent, makes chip fabrication process more loaded down with trivial details, consuming time.
The main component of cotton is organic fiber, is the common used material of apparel product; It is generally easy to get, cheap, convenient operation, and is applicable to forming multifarious micro-fluidic fluid passage, is prepare the one of backing material needed for microfluidic analysis chip newly to select.
2009, Wei Shen group proposed to utilize cotton thread to prepare microfluidic analysis chip technology (ACS Appl.Mater.Interfaces, 2010,2,1 – 6) first.In this work, the sewing of hydrophilic cotton thread forms microfluidic channel in super-hydrophobic fibrous substrate, or hydrophilic cotton thread is combined with paper and forms line-paper substrate compound micro-fluidic chip.
In the same year, a series of research (ACS Appl.Mater.Interfaces, 2010,2,1722 – 1728) has been done to line base microfluidic analysis chip by Whitesides group.This research work is also the chip preparation method adopting sewing, but has made a series of improvement compared to the research work of Wei Shen group, and has done correlative study to line imbibition characteristic, multiple sewing pattern.Since then, line base microfluidic analysis chip causes people as a kind of new diagnostic platform and greatly pays close attention to.
2011, Dhananjaya Dendukuri group utilized loom to be intertwined by line, made micro-fluidic immuno-chip (Lab on a Chip, 2011,11,2493-249).
In the same year, hydrophilic cotton thread and hydrophobic cotton thread are woven together according to different designs by the people such as Victor Breedveld, form fluid passage (ACS Appl.Mater.Intrefaces, 2011,3,3796-3803).
Above-mentioned research work is all utilize the natural capillary wick characteristic of cotton thread flow in hydrophilic channel to guide liquid and distribute, and which avoid extra External infusion sample adding system.But the preparation of these microfluidic analysis chips needs artificial braiding, winding usually, very low, the very difficult batch machining of its efficiency, and also this process technology is difficult to accurate detection and localization region, makes this class chip application there is the restriction of certain degree.Loom establishment technique can overcome the inefficient shortcoming of artificial establishment, but it is still difficult to accurate detection and localization region, and resolution ratio is low, often need professional to operate.
2012, the people such as Azadeh Nilghaz proposed a kind of wax transfer technique to process fabricbase microfluidic analysis chip, and its process is as follows: on paper, print required hydrophilic pathway pattern; By the dipped in liquid wax number minute of paper high-temperature fusion; Dry pattern designed by rear basis carefully to carve or cutting; Paraffin paper after cutting or engraving is covered on cotton, and put together and toast on hot plate, wax shifts from paper and penetrates on cotton, thus forms microfluidic analysis chip.The processing method of this fabricbase microfluidic analysis chip can detection and localization region more exactly, but still there are some defects: the pattern model on (1) paraffin paper needs artificial engraving or cutting, therefore be difficult to obtain accurate in size hydrophilic channel, and be difficult to the microfluidic analysis chip making stream complexity; (2) wax is transferred on cloth from paper, there is the problem of a transfer efficiency.This transfer efficiency is limited by many factors (as sheet type, paper and cloth exposure level, temperature etc.), therefore this processing method to paper, cloth even operating condition all have higher requirements; (3) last, this processing method is difficult to carry out high flux, high-resolution chip manufacture.
Summary of the invention
In order to overcome above-described defect, primary and foremost purpose of the present invention is to provide a kind of wax wire mark processing method of simple for production, with low cost, high flux fabricbase microfluidic analysis chip that applicability is wide, and the method can be used as a kind of new method that common laboratory prepares fabricbase microfluidic analysis chip.
Another object of the present invention is to provide the fabricbase prepared by said method microfluidic analysis chip.
Another object of the present invention is to provide the application by above-mentioned fabricbase microfluidic analysis chip.
Object of the present invention is achieved through the following technical solutions:
A wax wire mark processing method for fabricbase microfluidic analysis chip, comprises the following steps:
(1) cotton is placed in container, adds water, boil; Cotton is taken out, dries moisture, put into another container, add soaking in sodium carbonate solution; Then cotton is taken out, dry after rinsing with water;
(2) be laid on platform by the cotton after step (1) process, the serigraphy web plate with layout is covered on cotton, waxing, mills; Then web plate is toasted together with cotton, make wax melting infiltrate in cotton fully, toast and complete cotton to be separated with web plate, namely obtain fabricbase microfluidic analysis chip;
Described adds soaking in sodium carbonate solution, preferably soaks 5-10min;
Described serigraphy web plate is terylene grenadine material, and pattern software Adobe Illustrate CS5 designs;
Described wax can with commercially available wax crayon;
Described milling must be milled by the hard object with smooth surface, makes that wax is more is attached to cotton surface through web plate;
Described baking preferably toasts 5s on the heating plate of 75 DEG C.
The fabricbase microfluidic analysis chip obtained by said method can be used for biochemistry detection, especially can be used for the colorimetric detection of bovine serum albumin or glucose in urine.
The present invention has following advantage and effect relative to prior art:
1) method of the present invention does not need complicated main equipment, and only need water-bath and heating plate, these two kinds of instrumentations are fairly simple.
2) cotton that uses of the inventive method and wax all very cheap, wax is the wax crayon that general stationer's can buy.
3) method flow described in the invention is simple, does not need professional to operate.
4) the inventive method is directly coated on cotton by wax through web plate, then infiltrate in cotton through heating and melting, and do not need to be shifted by other media, therefore wax utilization ratio is high, effective.In addition, the chip that the inventive method is obtained has high flux, high-resolution feature.
Accompanying drawing explanation
Fig. 1 is the distribution schematic diagram of the obtained fabricbase microfluidic analysis chip of embodiment 1 and hydrophobic region and hydrophilic area.
Fig. 2 is the image of fabricbase microfluidic analysis chip its hydrophilic area after dripping cochineal solution of embodiment 1.
Fig. 3 is the web plate pattern utilizing software Adobe Illustrate CS5 to design in embodiment 2.
Fig. 4 is the schematic diagram of serigraphy web plate in embodiment 2.
Fig. 5 is the schematic diagram of the high flux fabricbase microfluidic analysis chip of embodiment 2.
Fig. 6 is the result of the test schematic diagram of fabricbase microfluidic analysis chip hydrophilic pathway resolution ratio.
Fig. 7 is the result of the test schematic diagram of fabricbase microfluidic analysis chip hydrophobic dam resolution ratio.
Fig. 8 is the colorimetric detection image in kind detecting bovine serum albumin in urine with fabricbase microfluidic analysis chip.
Fig. 9 is the graph of relation between the concentration of bovine serum albumin in urine and corresponding colorimetric intensity value.
Figure 10 is the colorimetric detection image in kind detecting glucose in urine with fabricbase microfluidic analysis chip.
Figure 11 is the graph of relation between the concentration of glucose in urine and corresponding colorimetric intensity value.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
A kind of wax wire mark processing method of fabricbase microfluidic analysis chip (hydrophilic region has 3 kinds of shapes), comprises the following steps:
1) getting about 0.8 square metre of cotton is put in beaker, adds 500mL ultra-pure water, and then putting into temperature is that the water-bath of 95 DEG C boils and boils 5min;
2) boil boil after cotton is taken out, put cotton in another beaker after water droplet is dry, add the sodium carbonate liquor that 500mL concentration is 10mg/mL, soak 5min;
3) after soaking in sodium carbonate solution, rinse with clear water, until cotton pH value is 6, under room temperature, dry 2h; The normal ph of human urine is about 6, aobvious faintly acid.Flushing is 6 to the pH value of cotton, being to ensure in the biochemistry detection in later stage, can not therefore occurring very large interference.
4) cotton after process is laid on platform, and is covered with the web plate with layout in the above, firmly compresses, prevents web plate or cotton from moving.Then, with wax crayon waxing, then mill with the spoon with smooth surface, make wax be attached to cotton surface through web plate;
5) after waxing is milled, cotton and web plate stick together, and keep cotton not to be separated with web plate, being placed on temperature is together that the heating plate of 75 DEG C toasts 5s, wax is fully infiltrated in cotton, is then separated with web plate by cotton, obtain fabricbase microfluidic analysis chip, as shown in Figure 1;
6) cochineal solution is dripped in the hydrophilic region of the fabricbase microfluidic analysis chip be processed at wax wire mark, find solution can be full of well whole hydrophilic region and and do not expand to hydrophobic region, as shown in Figure 2, thus demonstrate fabricbase microfluidic analysis chip wax wire mark success.
Embodiment 2
The wax wire mark processing method of high flux fabricbase microfluidic analysis chip
1. utilize web plate pattern that software Adobe Illustrate CS5 designs as shown in Figure 3, in pattern, black represents that wax can through web plate, and white represents that wax cannot through web plate.Be respectively on the rectangle painting canvas of 160mm, 120mm with height wide, devise the circular array of two kinds of different sizes.A kind of array is circular diameter is 8mm, and 4 row 12 arrange to be arranged; Another kind of array is circular diameter is 10mm, and 4 row 9 arrange to be arranged.
2. the web plate be made into according to web plate layout as shown in Figure 4, darkly in this web plate represents the impervious part of wax, correspond to and form hydrophilic region on cotton substrate; Light color represents the permeable part of wax, correspond to and form hydrophobic region on cotton substrate.The material of web plate is terylene grenadine, and housing is aluminium alloy.
3. utilize above-mentioned web plate, the array choosing diameter 8mm circle makes high flux fabricbase microfluidic analysis chip, and as shown in Figure 5, on chip, border circular areas is the micro-pond of hydrophily, and remainder is hydrophobic region.
Other concrete steps of processing method as described in Example 1.
Embodiment 3
The resolution ratio of fabricbase microfluidic analysis chip hydrophilic pathway
As shown in Figure 6, the hydrophilic pathway design width of fabricbase microfluidic analysis chip changes to 2.5mm (increasing progressively 0.2mm successively) from 0.9mm.When melt temperature and heat time are respectively 75 DEG C and 5s, cochineal dye solution all can be full of the hydrophilic pathway that design width is greater than 1.9mm.But, when melt temperature remains unchanged, only the heat time will be promoted to 10s and 15s, even if cochineal dye solution can not successfully be full of in the hydrophilic pathway of design width maximum (2.5mm).Therefore, the fabricbase microfluidic analysis chip hydrophilic pathway minimum widith of wax wire mark processing can reach 1.9mm.After the wax heating and melting of cotton surface, not only Vertical dimension cotton internal penetration, also spreads to surrounding.Further, in melt temperature one timing, the heat time is longer, and wax more trends towards toward surrounding diffusion.Along with the increase of heat time, the wax of hydrophobic region is more towards the diffusion of hydrophilic channel side, actual hydrophilic channel is narrowed, even can not form hydrophilic channel.
Other concrete steps of processing method as described in Example 1.
Embodiment 4
The resolution ratio on the hydrophobic dam of fabricbase microfluidic analysis chip
As shown in Figure 7, choose a series of hydrophobic dam design width, its scope changes to 450 μm from 50 μm, increases progressively 50 μm successively.When melt temperature and heat time are respectively 75 DEG C and 5s, design width is that the hydrophobic dam of 300 μm can prevent that stopping cochineal dye solution diffuses to another hydrophilic region from a hydrophilic region completely effectively.Although the hydrophobic dam that design width is less than 300 μm also can prevent dye solution from spreading once in a while, it shows instability.When wax wire mark processing conditions is set as 75 DEG C and 10s, the design width on minimum effective hydrophobic dam is reduced to 150 μm.When extending to 15s when heated further, the design width on minimum effective hydrophobic dam can reach 100 μm.As described in example 3, in melt temperature one timing, the heat time is longer, and wax more trends towards toward surrounding diffusion.Along with the increase of heat time, wax is more in the two side direction hydrophilic region diffusions on hydrophobic dam, and the width on actual hydrophobic dam is increased, and hinders the ability of liquid diffusion stronger.
Other concrete steps of processing method as described in Example 1.
Embodiment 5
For the colorimetric detection of bovine serum albumin, glucose in artificial urine, investigate the biochemistry detection application of fabricbase microfluidic analysis chip
The present embodiment adopts high flux fabricbase microfluidic analysis chip made in embodiment 2, and wherein the diameter of circular reaction tank is 8mm, and 4 row 12 arrange to be arranged, as shown in Figure 5.
1, in artificial urine, bovine serum albumin testing process is as follows:
1) in reaction tank (i.e. hydrophilic area), add 250mM (mM/l) citrate buffer (pH value 1.8) of 15 μ L, under room temperature, dry 1h;
2) add the 9mM Tetrabromophenol Blue solution of 5 μ L, under room temperature, dry 10min;
3) add the artificial urine solution of 3 μ L containing variable concentrations bovine serum albumin, obtain analysis chip scanned picture with flat bed scanner after drying 10min under room temperature, as shown in Figure 8;
4) picture is opened with softwares of Photoshop CS4, be converted into CMYK pattern, select cyan passage, select corresponding color development area, the average color intensity value that logging software provides automatically, recycling Origin 7.0 software data processing, obtains the relation curve between contained bovine serum albumin concentration in relative average color intensity value and artificial urine solution, as shown in Figure 9.
Fig. 8-9 to show in fabricbase microfluidic analysis chip energy colorimetric detection artificial urine the bovine serum albumin of 5 μMs of even more small concentrations.
2, in artificial urine, glucose detection process is as follows:
1) in reaction tank (i.e. hydrophilic area), add 0.6M (mol/L) liquor kalii iodide of 3 μ L, under room temperature, dry 10min;
2) add 3 μ L enzyme mixed solutions, this solution by 120U/mL (units per ml) glucose oxidase solution and 30U/mL horseradish peroxidase solution by volume 5:1 mix, dry 10min under room temperature; 3) add the artificial urine solution of 3 microlitres containing different glucose, after drying 10min under room temperature, obtain analysis chip scanned picture with flat bed scanner, as shown in Figure 10;
4) picture is opened with softwares of Photoshop CS4, be converted into 8 grayscale modes, select corresponding color development area, the average gray value that logging software provides automatically, recycling Origin 7.0 software data processing, obtain the relation curve between contained concentration of glucose in relative average gray value and artificial urine solution, as shown in figure 11.
Figure 10-11 shows 0.5mM glucose in fabricbase microfluidic analysis chip energy colorimetric detection artificial urine.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. a wax wire mark processing method for fabricbase microfluidic analysis chip, is characterized in that comprising the following steps:
(1) cotton is placed in container, adds water, boil; Cotton is taken out, dries moisture, put into another container, add soaking in sodium carbonate solution; Then cotton is taken out, dry after rinsing with water;
(2) be laid on platform by the cotton after step (1) process, the serigraphy web plate with layout is covered on cotton, waxing, mills; Then web plate is toasted together with cotton, toast and complete cotton to be separated with web plate, namely obtain fabricbase microfluidic analysis chip.
2. the wax wire mark processing method of fabricbase microfluidic analysis chip according to claim 1, is characterized in that: described adds soaking in sodium carbonate solution, is to soak 5-10min.
3. the wax wire mark processing method of fabricbase microfluidic analysis chip according to claim 1, is characterized in that: described serigraphy web plate is terylene grenadine material, and pattern software Adobe Illustrate CS5 designs.
4. the wax wire mark processing method of fabricbase microfluidic analysis chip according to claim 1, is characterized in that: described baking toasts 5s on the heating plate of 75 DEG C.
5. a fabricbase microfluidic analysis chip, is characterized in that: be prepared by the method described in any one of claim 1-4.
6. the application of fabricbase microfluidic analysis chip according to claim 5 in biochemistry detection.
7. the application in the colorimetric detection of fabricbase microfluidic analysis chip according to claim 5 bovine serum albumin or glucose in urine.
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| CN104569371A (en) * | 2015-01-29 | 2015-04-29 | 华南师范大学 | PDA modified paper-based micro-fluidic chip and application of PDA modified paper-based micro-fluidic chip to DNA colorimetric detection |
| CN104941705A (en) * | 2015-06-30 | 2015-09-30 | 华南师范大学 | Three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and preparation method and application thereof |
| CN106485250A (en) * | 2016-09-09 | 2017-03-08 | 华东理工大学 | A kind of recognition methods of paper chip gray value and its application |
| CN106546578A (en) * | 2016-10-28 | 2017-03-29 | 华南师范大学 | The gravity on content of wax dam/capillary flow cloth chip and its application in glucose sensing |
| CN106378214B (en) * | 2016-08-29 | 2018-10-30 | 华南师范大学 | Open U-shaped bipolar electrode electrogenerated chemiluminescence cloth chip and its preparation method and purposes |
| CN112192941A (en) * | 2020-09-30 | 2021-01-08 | 华南师范大学 | Wax screen printing machine |
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| CN112192941A (en) * | 2020-09-30 | 2021-01-08 | 华南师范大学 | Wax screen printing machine |
| CN112192941B (en) * | 2020-09-30 | 2021-10-08 | 华南师范大学 | Wax screen printing machine |
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